KR101827856B1 - Manufacturing method of multifunctional liquid type nutrition cleaner composition for improving soil, nutritionizing plant and removing soil contaminant - Google Patents

Abstract

The present invention provides a method of manufacturing a convenient, eco-friendly, and excellent liquefied nourishing cleaner composition for removing soil pollutants. According to the present invention, the liquefied nourishing cleaner composition is able to be used immediately in a field since the composition does not require a liquefaction procedure in which the composition is diluted in water. Moreover, according to the present invention, the manufacturing method is capable of simplifying manufacturing processes and reducing costs by omitting an organic matter solidifying procedure. In addition, according to the present invention, since the liquefied nourishing cleaner composition contains a lactobacillus plantarum culture medium at a high concentration, the present invention is capable of activating soil within a short period, having excellent performance of removing soil pollutants such as salt or a deicing agent, and improving soil for the growth and development of plants through a sufficient supply of necessary nutrients.

Description

Technical Field [0001] The present invention relates to a method for preparing a liquid nutrient cleaner composition for multifunctional soil improvement, plant nutrition supply and soil pollutant removal,

The present invention relates to a method for preparing a liquid nutrient cleaner composition for removing soil pollutants, and more particularly, to a method for preparing a liquid nutritious cleaner composition for soil pollutant removal, which comprises neutralizing excessively accumulated snow remnant in the soil, washing polluted plants, The present invention also provides a method for producing a liquid nutritional cleaner composition for removing soil pollutants, which is capable of improving the soil physically and chemically properties of the soil by improving the acidity of the soil.

Since the Industrial Revolution, ecosystems are faced with serious environmental pollution problems due to population increase and adverse effects of industrial activities. In recent years, environmental pollution such as soil, air and water pollution is increasing due to the rapid increase of fossil fuel use such as automobiles . Soil pollution in environmental pollution is characterized by the fact that once the pollution is compared with air pollution or water pollution, purification treatment is relatively much more difficult and the influence of pollution lasts for a long time. One example of such environmental pollution is the urban roads. In general, the avenue offers beautiful scenery, helps the mental health of the citizens, and cleans the polluted air. However, due to the environmental pollution of urban ecosystems, the growth of tree species is impeded or severe deaths occur. The above-mentioned environmental pollution is caused by various causes.

For example, in recent years, calcium chloride (CaCl ₂ ) and sodium chloride (NaCl) are separately sprayed on roads to prevent winter snow removal and road icing, or sodium chloride (NaCl) is added to a calcium chloride (CaCl ₂ ) Dissolve and spray. These compounds, such as calcium chloride (CaCl ₂ ) and sodium chloride (NaCl), which are used as snow remover, are one of the major causes of environmental pollution. When snow removers are sprayed on the road, the ingredients contained in the snow remover may scatter directly on the trunks or leaves of the trees and cause direct damage. In addition, the chlorine component flows into the soil in a liquid state and accumulates, resulting in ion imbalance in the soil. In addition, the snow remover can reverse the osmotic action of the roots, inhibiting the growth of the trees or even destroying them. The reason for using compounds such as calcium chloride (CaCl ₂ ) or sodium chloride (NaCl) as a snow removing agent is that these compounds have deliquescence that absorbs moisture in the air or absorbs the surrounding water. When these compounds are disassociated, heat is generated, and snow is melted by the heat of fusion (or heat of dissolution), and the snow is removed. Water that melts snow again dissolves calcium chloride or sodium chloride to generate heat. Water containing calcium chloride has a freezing point as low as about -50 ° C due to freezing point depression, so it does not freeze well. However, when a snow remover such as calcium chloride (CaCl ₂ ) or sodium chloride (NaCl) is sprayed, corrosion of chlorine components causes corrosion of automobiles, reinforcing bars of buildings, roadside facilities (street lamps, traffic lights, fire hydrants). Particularly, the lower end portions of automobiles and facilities in direct contact with the snow remover are easily corroded. The aqueous calcium chloride solution remains on the road for a considerable time after the water is evaporated due to the delayed evaporation of water, and has a slippery property, which may cause traffic accidents. In addition, plants such as roadside trees and lawns are susceptible to failure by direct contact with snow removers or by snow removers scattered by automobiles.

In order to solve such a problem, Korean Patent Registration No. 10-0329625 and Korean Patent Registration No. 10-1043581 disclose a cleaning agent for removing contaminants from plants or soil. However, the plant and soil cleansing agents disclosed in Korean Patent Publication No. 10-0329625 and Korean Patent Registration No. 10-1043581 are limited to the removal of the snow remover or the removal of contaminants, Or inorganic fertilizer, it is insufficient to supply sufficient nutrients to the plant, and the soil improvement effect such as changing the physico-chemical properties of the soil to be suitable for plant growth is insufficient. Korean Patent Registration No. 10-1373323 discloses a method for producing an organic material mixture comprising a vegetable organic material including bark and sawdust and an animal organic material including stem and slaughter byproducts, Bacillus subtilis, Bacillus licheniformis and Bacillus megaterium, and at least one microorganism selected from the group consisting of Rhodobacter capsulata or Rhodobacter spp. sphaeroides, and then fermented for 15 to 40 days in a fermentation tank under aerobic conditions and secondary fermentation for 60 to 120 days in a non-fermented state to produce fermented organic matter ; Bacillus subtilis, Bacillus licheniformis, and Bacillus megaterium are added to a fermentation organic material mixture obtained by mixing the fermentation organic material and water. Adding a secondary mixed microorganism, holding it for 20 to 30 days under aerobic conditions, concentrating and filtering to obtain a fermented organic concentrate; Adding a coagulant to the fermented organic substance concentrate to aggregate the fermented organic material into a cake state and then drying to obtain a solidified fermentation organic material; Pulverizing the solidified fermentation organic material to obtain a fermentation organic material powder; And a step of adding a nitrogen-containing fertilizer powder, a phosphorus-containing fertilizer powder, a potassium-containing fertilizer powder, a cation neutralizer, a trace element-containing substance necessary for plant growth, a surfactant, an anion neutralizer, a plant growth regulator and an acidity- To obtain a final composition in the solid phase form. ≪ RTI ID = 0.0 > A < / RTI > However, since the nutritional cleaner composition disclosed in Korean Patent Publication No. 10-1373323 is in a solid form, it takes additional time and additional time for spraying in consideration of the method of using the nutritional cleaner composition which is uniformly dissolved in water and sprayed with liquid, There is a problem in that the nutrient supply effect necessary for the growth of the plant is not high.

SUMMARY OF THE INVENTION The present invention has been made under these circumstances, and it is an object of the present invention to provide a method of neutralizing a snow removing agent which is more environmentally friendly than a conventional plant and soil cleansing agent, neutralization of a snow accumulating agent in soil, washing of polluted plants, A method of manufacturing a liquid nutrient cleaner composition for soil pollutant removal, which can improve the soil so as to be advantageous for the growth of plants by changing the physical and chemical properties of the soil as well as the nutrient supply and acidity correction effect necessary for soil pollutants, have.

The inventor of the present invention has developed a nutrient cleaner composition for removing soil pollutants and applied for and registered them (Korean Patent Registration No. 10-1373323). However, the nutrient cleaner composition for removing soil contaminants disclosed in the existing patented invention has a high- It has become necessary to add a process of uniformly diluting the liquid into a liquid state in order to use it as a form, and thus it is difficult to use and immediately use it. The inventors of the present invention have found that the nutrient cleaner composition for removing soil contaminants disclosed in the existing patent invention has a limited amount of nutrients to be supplied for plant growth and thus has limitations in improving plant growth. The inventor of the present invention has made some improvements to the method of manufacturing the nutrient cleaner composition for removing soil contaminants disclosed in Korean Patent Registration No. 10-1373323, and it is easy to use, and the amount of nutrients required for plant growth is increased, A liquid nutritional cleaner composition was completed.

In order to attain the above object, the present invention provides a method for producing Lactobacillus plantarum , comprising: inoculating Lactobacillus plantarum seeds to a liquid medium and culturing under anaerobic conditions to obtain a Lactobacillus plantarum culture; A nitrogen-containing substance, a potassium-containing substance, a phosphorus-containing substance, a trace element-containing substance necessary for plant growth, a surfactant, a plant growth regulator, a cationic neutralizer and an anionic neutralizer are added to and mixed with the lactobacillus plantarum There is provided a method for preparing a liquid nutritious cleaner composition for soil pollutant removal comprising the steps of: At this time, the step of obtaining the final composition in liquid form includes, for example, (a) using a culture medium of Lactobacillus plantarum as a primary basic substance, adding a nitrogen-containing substance, a potassium-containing substance and a phosphorus- Producing a secondary basis material; (b) adding a trace element-containing material necessary for plant growth to the secondary base material and mixing the same to prepare a tertiary base material; And (c) adding a surfactant, a plant growth regulator, a cationic neutralizer, and an anionic neutralizer to the tertiary basic substance and mixing them to prepare a final composition in liquid form.

The liquid nutritional cleaner composition for removing soil contaminants according to the present invention does not require a process of uniformly diluting the water into a liquid state, so that it can be used immediately in the field. In addition, the method of manufacturing a liquid nutrient cleaner composition for removing soil pollutants according to the present invention can simplify a manufacturing process and reduce costs because a process of solidifying an organic material is omitted. Also, since the liquid nutritional cleaner composition for removing soil pollutants according to the present invention contains a high concentration of Lactobacillus plantarum culture liquid, the soil can be activated in a short period of time, and the removal of soil pollutants such as salt, The soil can be improved to provide the plant with excellent performance and sufficient nutrients necessary for the plant.

1 is a photograph showing turbidity of a filtrate obtained by treating a contaminated plant with tap water or a nutrient cleaner composition in an embodiment of the present invention.
FIG. 2 is a photograph showing the rapeseed growth rate after 20 days of sowing treated with commercially available soil remediation product or nutritional cleaner composition after rapeseed seeding in the embodiment of the present invention.

Hereinafter, the present invention will be described in detail. One aspect of the present invention is to neutralize excessively accumulated snow remover in the soil and to wash the plant contaminated with pollution as a main function, and has a side effect such as foliar fertilization, supply of nutrients required for plants, acidity correction, The present invention relates to a nutrient cleaner composition for removing multifunctional soil pollutants, which can improve the soil properties of plants to improve their physicochemical properties.

The nutrient cleaner composition for removing soil pollutants according to the present invention is characterized by comprising a culture medium containing lactobacillus plantarum, a nitrogen-containing substance, a potassium-containing substance, a phosphorus-containing substance, a trace element-containing substance required for plant growth, a surfactant, Based neutralizing agent and an anionic neutralizing agent. At this time, the Lactobacillus plantarum was obtained by inoculating Lactobacillus plantarum seedlings into a liquid medium and culturing under anaerobic conditions. The details will be described in a method of manufacturing a nutrient cleaner composition for removing soil pollutants to be described later.

The content of the components constituting the nutrient cleaner composition for removing soil contaminants according to the present invention may vary depending on the kind of the pollutants accumulated in the soil, the degree of pollution of the soil, the kind of the pollutant attached to the plant, The nutrient cleaner composition for removing soil contaminants according to the present invention may comprise 40 to 60% by weight of a culture medium of lactobacillus plantarum, 15 to 35% by weight of a nitrogen-containing material, The content of the phosphorus-containing substance is from 0.5 to 5% by weight, the content of the trace element-containing substance is from 1 to 6% by weight, the surfactant is from 2 to 8% by weight, the plant growth regulator is from 0.2 to 3% 0.1 to 2% by weight of a cationic neutralizer and 0.1 to 2% by weight of an anionic neutralizer. The nutritional cleaner composition for removing soil contaminants according to the present invention may further comprise, for example, 45 to 55% by weight of a lactobacillus plantarum culture, 20 to 30% by weight of a nitrogen-containing material, 9 to 17% by weight of a phosphorus-containing substance, 1 to 4% by weight of a phosphorus-containing substance, 2 to 5% by weight of a trace element-containing substance necessary for plant growth, 3 to 7% by weight of a surfactant, 0.5 to 2% by weight of a plant growth regulator, 0.2 to 1% by weight of an anionic neutralizing agent and 0.2 to 1% by weight of an anionic neutralizing agent.

The above-mentioned Lactobacillus plantarum liquid contains a predetermined concentration of Lactobacillus plantarum and activates the soil through actions such as promotion of inclusion, improvement of physical properties, desalination, nitrogen fixation, hyperion neutralization and the like.

The nitrogen-containing material is a substance that supplies nitrogen to plants in the form of ions, and is preferably one or more selected from the group consisting of urea, ammonium sulfate, ammonium nitrate, ammonium chloride, ammonium phosphate, sodium nitrate, . A more preferred example of the nitrogen-containing substance is an element.

The potassium-containing material is a substance that supplies potassium to plants in ionic form, and may be composed of at least one selected from the group consisting of potassium hydroxide, potassium sulfate, potassium nitrate, potassium chloride, and potassium phosphate. The potassium phosphate may be selected from potassium phosphate monobasic (KH ₂ PO ₄ ), potassium phosphate dibasic (K ₂ HPO ₄ ), or potassium tertiary phosphate (K ₃ PO ₄ ). A more preferred example of the potassium containing material is potassium secondary phosphate.

The phosphorus-containing material is a substance which supplies phosphorus in the form of ions to plants, and may be composed of at least one selected from the group consisting of phosphoric acid, potassium phosphate, ammonium phosphate, calcium phosphate, sodium phosphate and superphosphate . The potassium phosphate may be selected from potassium phosphate monobasic (KH ₂ PO ₄ ), potassium phosphate dibasic (K ₂ HPO ₄ ), or potassium tertiary phosphate (K ₃ PO ₄ ). A more preferred example of the phosphorus-containing substance is phosphoric acid.

The trace element-containing substance is a substance that provides a trace element necessary for plant growth, for example, a boron-containing substance, a zinc-containing substance, an iron-containing substance, a manganese-containing substance, a copper-containing substance, a calcium- Magnesium-containing materials, and the like. In particular, in consideration of the influence on plant growth, it is more preferable that the trace element-containing substance includes a boron-containing substance, a zinc-containing substance and a molybdenum-containing substance. To the boron-containing material to may the borax, boric acid, zinc-containing material is _{Zn (OH) 2, ZnSO 4} , the _{Fe (OH) 2, Fe (} OH) with ZnCl ₂ and the like, iron-containing substance _3, and the like FeCl _2, FeCl _3, FeSO _4, is a manganese-containing material, such as _{Mn (OH) 2, MnCl 2} , MnSO 4 a, and is a copper-containing material, such as _{Cu (OH) 2, CuCl 2} , CuSO 4 (NH ₄ ) ₆ Mo ₇ O ₂₄ and the like can be used as the molybdenum-containing material. The trace element-containing material may be composed of borax, zinc sulfate and ammonium molybdate as a preferable example.

The surfactant is not limited in its kind insofar as it is capable of removing contaminants adhered to plants and is harmless to plants and has no environmental pollution, and may be any of known anionic surfactants, cationic surfactants, amphoteric surfactants, Non-ionic surfactants, and the like. The anionic surfactant may be selected from the group consisting of carboxylic acid salts, sulfonic acid salts (e.g., alkylbenzenesulfonic acid salts,? -Olefin sulfonic acid salts, alkane sulfonic acid salts), sulfuric acid ester salts (for example, alkylsulfuric acid ester salts, alkyl ether sulfuric acid ester salts) , A phosphoric acid ester salt, a phosphonic acid salt, and the like. The cationic surfactant may be selected from an amine salt (for example, polyoxyethylene alkylamine), a quaternary alkylammonium salt, a phosphonium salt, a sulfonium salt, The surfactant may be selected from an amino acid type, a betaine type, and the like. The nonionic surfactant may be selected from polyethylene glycol derivatives, polyhydric alcohol derivatives (for example, fatty acid monoglycerin esters, fatty acid polyglycol esters, fatty acid sorbitan esters, fatty acid sucrose esters). Further, in the present invention, the surfactant may include a silicone surfactant. Specific examples of the surfactant include (C ₂ H ₅ ) ₃ Si (CH ₂ ) n COOH wherein n is an integer selected from 1 to 10, (CH ₃ ) ₃ SiC ₆ H ₄ (OC ₂ H ₄ ) nOH [n is an integer], which is selected from 1 to _{10, [(CH 3) 3} SiO] 3 Si (CH 2) 3 NH (CH 2) 3 NH (CH 2) 2 NH 2, C 6 H 6 (CH 3) 2 Si _{(CH 2) COOH, HOOCC 2} C 2 H 4 Si (CH 3) 2 SiC 2 H 4 COOH, [(CH 3) SiO] 2 Si (CH 3) (CH 2) 3 OSO 3 H, lauryl amino propylene An alkyl ether sulfonic acid salt, an alkyl amide sulfonic acid salt, an alkane sulfonic acid salt, an Igepon T (N-acyl-N-myrthyltaurin salt), an alkyl sulfonic acid salt, , Potassium salt of fatty acid, HO (CH ₂ H ₄ O) SiC ₂ H ₄ COO (C ₂ H ₄ O) ₃ H, alkali metal salt of Coconut Oil Fatty Acid / Isethionic acid copolymer, Sodium Cocoyl Isethionate, Ammonium Cocoyl Isethionate, and the like are preferred. It is _{[(CH 3) 3 SiO]} 3 Si (CH 2) 3 NH (CH 2) 3 NH (CH 2) 2 NH 2, C 6 H 6 (CH 3) 2 Si (CH 2) COOH, HOOCC 2 C _{2 H 4 Si (CH 3)} 2 SiC 2 H 4 COOH, [(CH 3) SiO] 2 Si (CH 3) (CH 2) 3 OSO 3 H, lauryl amino propylene acid methyl, lauryl sulfate, alkyl esters of (N-acyl-N-myrthyltaurin salt), fatty acid potassium, HO (CH ₂ H (CH ₃ ) ₂ ), alkylbenzenesulfonic acid salts, alkylbenzenesulfonic acid salts, ₄ O) SiC ₂ H ₄ COO (C ₂ H ₄ O) ₃ H, alkali metal salts of Coconut Oil Fatty Acid / Isethionic acid copolymer, sodium cocoyl isethionate ) And ammonium cocoyl isethionate (Ammonium Cocoyl Isethionate). The surfactant may be composed of sodium salt of coconut oil fatty acid / isethionic acid copolymer and sodium cocoyl isethionate as a more preferable example.

The plant growth regulator may be composed of at least one kind selected from plant growth hormones that affect plant growth. Examples of the plant growth hormone include indole acetic acid (or auxin), gibberellin, cytokinin, florigen, brassinosteroid, and the like, preferably Gibberellin.

The cationic neutralizing agent is a chlorine ion (Cl ^-) as a substance that provides a cation which can be fixed ^{to, Mg 2 +, K +,} NH 4 +, NH 3 +, Fe 3 +, Fe 2 +, and Al ^{3 +} Or the like. These cationic neutralizing agents are preferably MgB ₂ , Mg (CO ₃ ) ₂ , NH ₄ OH, (NH ₄ ) ₂ SO ₄ , (NH ₄ ) ₂ S ₂ O ₈ , (NH ₄ ) ₂ CO ₃ , _{4) 3 PO 4, NH 4} HCO 3, (NH 4) 2 CO 3 H 2 O, KBr, K 2 CO 3, K 3 [Co (NO 2) 6], Fe 3 O 4, Fe (CH 3 CO ₂ ) ₂ , K ₂ SO ₄ , Fe (OH) ₃ , FePO ₄ , Fe (C ₆ H ₁₁ O ₇ ) ₂ , FeC ₂ O ₄ , FeSO ₄ , Al ₂ O ₃ B ₂ O ₃ , AlPO ₄ , Al (OH) ₃ , Al ₂ O ₃ and Al ₂ (SO ₄ ) _{3. In} consideration of the immobilization effect of chlorine ions, KBr, K ₂ CO ₃ , K ₃ [ Co (NO ₂ ) ₆ ] and K ₂ SO ₄ , and most preferably K ₂ CO ₃ .

The anion neutralizer is a substance which provides an anion capable of fixing calcium ion (Ca 2+) or sodium ion (Na +), and at least one of BO ₃ ^3- , SO ₄ ^2- , NO ₃ ^- , OH ^- and COO ^- Or more. These anionic neutralizers are preferably H ₃ BO ₃ , H ₂ SO ₃ , H ₂ S ₂ O ₇ , H ₂ SO ₅ , H ₂ S ₂ O ₈ , C ₄ H ₈ SO ₂ , H ₂ SO ₄ , NH _{4 OH, (NH 4) 2} SO 4, K 2 SO 4, Fe (OH) 3, FeSO 4, Al (OH) 3 and Al ₂ (SO ₄₎ be composed of at least one element selected from the group consisting of ₃ And H ₃ BO ₃ is more preferable when the immobilization effect is considered.

The nutritional cleaner composition for removing soil contaminants according to the present invention preferably comprises 40 to 60% by weight of a culture medium of Lactobacillus plantarum, 15 to 35% by weight of urea, 6 to 20% by weight of potassium phosphate, 0.5 to 5 wt% of phosphorus-containing material, 0.5 to 5 wt% of borax, 0.1 to 2 wt% of zinc sulfate, 0.1 to 2 wt% of ammonium molybdate, 2 to 8 wt% of surfactant, 0.2 to 3 wt% 0.1 to 2% by weight of a cationic neutralizer, and 0.1 to 2% by weight of an anionic neutralizer. The nutritional cleaner composition for removing soil contaminants according to the present invention may further contain 45 to 55% by weight of a lactobacillus plantarum culture, 20 to 30% by weight of urea, 9 to 30% by weight of urea, 1 to 4% by weight of phosphoric acid, 1 to 4% by weight of borax, 0.2 to 1% by weight of zinc sulfate, 0.2 to 1% by weight of ammonium molybdate, 1 to 4% by weight of coconut oil fatty acid / isethionic acid ) Of sodium carbonate and sodium cocoyl isethionate, 0.5 to 2% by weight of gibberellin, 0.2 to 1% by weight of potassium carbonate and 0.2 to 1% by weight of boric acid, % ≪ / RTI > by weight.

The nutritional cleaner composition for removing soil contaminants according to the present invention can be used in the form of a liquid form, in which the soil pollutants are removed and the soil is improved immediately, or the soil is sprayed on the surface of the plant. It may be diluted with water to about 20 to 200 times, preferably 50 to 100 times, and then may be used in a manner of irrigating the soil or spraying on the surface of a plant.

One aspect of the present invention relates to a method for preparing a liquid nutritional cleaner composition for removing multifunctional soil contaminants. A method for preparing a liquid nutritional cleaner composition for removing soil pollutants according to the present invention comprises the steps of: inoculating a liquid medium with Lactobacillus plantarum seeds and culturing under anaerobic conditions to obtain a Lactobacillus plantarum culture; A nitrogen-containing substance, a potassium-containing substance, a phosphorus-containing substance, a trace element-containing substance necessary for plant growth, a surfactant, a plant growth regulator, a cationic neutralizer and an anionic neutralizer are added to and mixed with the lactobacillus plantarum And finally obtaining a final composition in liquid form.

 In the method for producing a liquid nutritious cleaner composition for removing soil pollutants according to the present invention, the step of obtaining the final composition in liquid form is preferably carried out by (a) using a culture medium of Lactobacillus plantarum as a primary starting material, Adding and mixing the substance, the potassium-containing substance and the phosphorus-containing substance to prepare a secondary basic substance; (b) adding a trace element-containing material necessary for plant growth to the secondary base material and mixing the same to prepare a tertiary base material; And (c) adding a surfactant, a plant growth regulator, a cationic neutralizer, and an anionic neutralizer to the tertiary basic substance and mixing them to prepare a final composition in liquid form.

In the method for preparing a liquid nutritious cleaner composition for removing soil pollutants according to the present invention, the liquid medium is prepared by mixing and dissolving 1.5 to 3.5 parts by weight of a microorganism medium per 100 parts by weight of water, preferably 2 to 3 parts by weight The mixture is mixed and dissolved. The microbial medium may be in the form of a composition containing molasses, glucose, sodium glutamate, and yeast extract. Preferably, the microbial medium contains 40 to 60 wt% of molasses, 10 to 30 wt% of glucose, 5 to 15 wt% of sodium glutamate By weight based on the total weight, 45 to 55% by weight of molasses, 15 to 25% by weight of glucose, 8 to 25% by weight of sodium glutamate, 2 to 8% by weight of yeast extract, 12% by weight of yeast extract, 3-7% by weight of yeast extract, and water as the balance.

In the method for producing a liquid nutritious cleaner composition for removing soil pollutants of the present invention, the Lactobacillus plantarum strain is preferably a solid phase strain having 10 ⁷ to 10 ⁹ Lactobacillus plantarum count per unit g, More preferably a solid phase strain having 10 ⁸ to 10 ⁹ Lactobacillus plantarum per unit g. The inoculum amount of the Lactobacillus plantarum seed is preferably 0.004 to 0.06 part by weight per 100 parts by weight of the liquid medium and more preferably 0.008 to 0.04 part by weight per 100 parts by weight of the liquid medium.

In the method for producing a liquid nutritious cleaner composition for removing soil pollutants according to the present invention, the anaerobic incubation temperature of Lactobacillus plantarum is 30 to 35 ° C, preferably 31 to 34 ° C. In addition, the anaerobic incubation time of Lactobacillus plantarum is 72-96 hr, preferably 75-90 hr.

In the method for producing a liquid nutritious cleaner composition for removing soil pollutants of the present invention, the concentration of the lactobacillus plantarum of the lactobacillus plantarum is preferably 10 ⁵ to 10 ⁸ / ml, more preferably 10 ⁶ to 10 ⁷ cells / ml.

In the method for producing a liquid nutrient cleaner composition for removing soil pollutants according to the present invention, the nitrogen-containing material is composed of at least one element selected from the group consisting of urea, ammonium sulfate, ammonium nitrate, ammonium chloride, ammonium phosphate, sodium nitrate, , ≪ / RTI >

 In the method for producing a liquid nutritious cleaner composition for removing soil contaminants according to the present invention, the potassium containing material is composed of at least one selected from the group consisting of potassium hydroxide, potassium sulfate, potassium nitrate, potassium chloride and potassium phosphate, .

In the method for producing a liquid nutritious cleaner composition for removing soil pollutants according to the present invention, the phosphorus-containing substance is composed of at least one member selected from the group consisting of phosphoric acid, potassium phosphate, ammonium phosphate, calcium phosphate, sodium phosphate and superphosphate, .

In the method for producing a liquid nutrient cleaner composition for removing soil pollutants according to the present invention, the trace element-containing material necessary for plant growth is selected from the group consisting of a boron-containing material, a zinc-containing material, an iron-containing material, a manganese- A molybdenum-containing material, and a magnesium-containing material, and is preferably composed of borax, zinc sulfate, and ammonium molybdate.

The surfactant in removing soil contaminants of the present invention a liquid nutritional cleaner composition preparation method is _{[(CH 3) 3 SiO]} 3 Si (CH 2) 3 NH (CH 2) 3 NH (CH 2) 2 NH 2, C _{6 H 6 (CH 3) 2} Si (CH 2) COOH, HOOCC 2 C 2 H 4 Si (CH 3) 2 SiC 2 H 4 COOH, [(CH 3) SiO] 2 Si (CH 3) (CH 2) ₃ OSO ₃ H, lauryl methyl aminopropionate, sodium lauryl sulfate, alkylester sulfonate, alkylbenzene sulfonate,? -Olefin sulfonate, alkyl ether sulfate ester, alkylamide sulfonate, alkane sulfonate, Igepon T N-acyl-N-myrthyltaurin salt), potassium fatty acid, HO (CH ₂ H ₄ O) SiC ₂ H ₄ COO (C ₂ H ₄ O) ₃ H, Coconut Oil Fatty Acid / Isethionic (Coconut Oil), which is composed of at least one member selected from the group consisting of alkali metal salts of sodium alginate, sodium cocoyl isethionate and ammonium cocoyl isethionate, Fatty Ac id / isethionic acid copolymer and sodium cocoyl isethionate are preferable.

In the method for producing a liquid nutritional cleaner composition for removing soil pollutants according to the present invention, the plant growth regulator is selected from plant growth hormones such as indole acetic acid (or oxine), gibberellin, cytokinin, florigen, brassinosteroid and the like It is preferably composed of at least one kind, and is gibberellin.

In the method for preparing a liquid nutrient cleaner composition for removing soil pollutants according to the present invention, the cationic neutralizer is MgB ₂ , Mg (CO ₃ ) ₂ , NH ₄ OH, (NH ₄ ) ₂ SO ₄ , (NH ₄ ) ₂ S ₂ O _{8, (NH 4) 2 CO} 3, (NH 4) 3 PO 4, NH 4 HCO 3, (NH 4) 2 CO 3 H 2 O, KBr, K 2 CO 3, K 3 [Co (NO 2) 6 , Fe ₃ O ₄ , Fe (CH ₃ CO ₂ ) ₂ , K ₂ SO ₄ , Fe (OH) ₃ , FePO ₄ , Fe (C ₆ H ₁₁ O ₇ ) ₂ , FeC ₂ O ₄ , FeSO ₄ , Al ₂ O ₃ B ₂ O ₃ , AlPO ₄ , Al (OH) ₃ , Al ₂ O ₃ and Al ₂ (SO ₄ ) ₃ , and is preferably K ₂ CO ₃ .

In the method for preparing a liquid nutrient cleaner composition for removing soil contaminants according to the present invention, the anionic neutralizer may be selected from the group consisting of H ₃ BO ₃ , H ₂ SO ₃ , H ₂ S ₂ O ₇ , H ₂ SO ₅ , H ₂ S ₂ O ₈ , C _{4 H 8 SO 2, H 2} SO 4, NH 4 OH, (NH 4) 2 SO 4, K 2 SO 4, Fe (OH) 3, FeSO 4, Al (OH) 3 and Al ₂ (SO _{4) 3} , And is preferably H ₃ BO ₃ .

In the method for producing a liquid nutritious cleaner composition for removing soil pollutants according to the present invention, the final composition in liquid form in the method comprises 40 to 60% by weight of a lactobacillus plantarum culture liquid, 15 to 35% by weight of a nitrogen- The content of the phosphorus-containing substance is 0.5 to 5% by weight, the content of the trace element-containing substance required for plant growth is 1 to 6% by weight, the surfactant is 2 to 8% by weight, the plant growth regulator is 0.2 to 3% 0.1 to 2% by weight of a neutralizing agent and 0.1 to 2% by weight of an anionic neutralizing agent, preferably 45 to 55% by weight of a lactobacillus plantarum culture, 20 to 30% by weight of a nitrogen- A plant growth regulator, 0.5 to 2% by weight of a plant growth regulator, 9 to 17% by weight of a potassium containing material, 1 to 4% by weight of a phosphorus containing substance, 2 to 5% 0.2 to 1% by weight of a cationic neutralizer and 0.2% by weight of an anionic neutralizer To 1% by weight. In addition, the final composition in liquid form preferably comprises 40-60 wt.% Of a Lactobacillus plantarum culture, 15-35 wt.% Of urea, 6-20 wt.% Of dibasic potassium phosphate, The present invention relates to a method for producing a plant growth regulator, comprising the steps of: 0.5 to 5 wt% of a material; 0.5 to 5 wt% of borax; 0.1 to 2 wt% of zinc sulfate; 0.1 to 2 wt% of ammonium molybdate; 2 to 8 wt% 0.1 to 2% by weight of a neutralizing agent and 0.1 to 2% by weight of an anionic neutralizing agent, most preferably 45 to 55% by weight of a lactobacillus plantarum culture, 20 to 30% by weight of an element, (Coconut Oil Fatty Acid) / Isechi Co., Ltd.), Potassium phosphate 9-17 wt%, phosphoric acid 1-4 wt%, borax 1-4 wt%, zinc sulfate 0.2-1 wt%, ammonium molybdate 0.2-1 wt% Surfactants composed of the sodium salt of an isethionic acid copolymer and sodium cocoyl isethionate 3 to 7 0.5 to 2% by weight of gibberellin, 0.2 to 1% by weight of potassium carbonate and 0.2 to 1% by weight of boric acid.

Hereinafter, the present invention will be described in more detail with reference to examples. However, the following examples are intended to clearly illustrate the technical features of the present invention and do not limit the scope of protection of the present invention.

1. Nutrition for removing soil pollutants Cleaner  Preparation of composition

Production Example 1

The culture medium was prepared as follows: medium (1,000 g) of water (composition: 50 g of molasses, 20 g of glucose, 10 g of sodium glutamate, 5 g of yeast extract, 14 g of purified water and 1 g of other ingredients based on 100 g of medium; Korea) was added and mixed. Then, 200 g of Lactobacillus plantarum solid phase strain (microorganism concentration: about 10 ⁸ / g) was inoculated therein, and the mixture was incubated at about 33 ± 1 ° C. for about 80 hours for anaerobic And cultured to obtain a culture of Lactobacillus plantarum. The Lactobacillus plantarum concentration in the Lactobacillus plantarum was about ¹⁰⁶ / ml.

25 parts by weight of urea, 13 parts by weight of potassium phosphate and 2 parts by weight of phosphoric acid were added to and mixed with 50 parts by weight of the primary basic substance as a primary starting material of the lactobacillus plantarum culture to prepare a secondary basic substance Respectively. Then, 2 parts by weight of borax, 0.5 part by weight of zinc sulfate and 0.5 g of ammonium molybdate were added to the secondary basic material and mixed to prepare a tertiary base material. Thereafter, 5 parts by weight of a natural surfactant (a mixture of sodium cocoyl isethionate and a natural detergent for fruit washing), 1 part by weight of gibberellin as a plant growth regulator, 0.5 part by weight of potassium carbonate as a cationic neutralizing agent And 0.5 parts by weight of boric acid as an anionic neutralizing agent were mixed to prepare a nutrient cleaner composition for removing soil contaminants in liquid form. The natural detergent constituting the natural surfactant is a sodium salt of a coconut oil fatty acid / isethionic acid copolymer.

Comparative Production Example 1

40 parts by weight of slaughter by-products, 20 parts by weight of grain, 20 parts by weight of bark, and 20 parts by weight of sawdust were placed in a rotary fermenter, followed by addition of Bacillus subtilis licheniformis ), Bacillus megaterium megaterium and Rhodobacter sphaeroides , and then primary fermentation was carried out for about 15 days to obtain a primary fermentation organism. During the first fermentation, the aerobic condition was maintained by using the oxygen supply device provided at the bottom of the fermenter, and the organic material mixture and the mixed microorganism were evenly stirred with the impeller provided in the fermenter. During the first fermentation, the temperature profile in the fermenter initially remained below 40 ° C, then gradually increased to 75 ° C. The first fermented organic matter had a water content of about 60%, and the acidity was weakly alkaline. The first fermented organic material was taken out of the fermenter, allowed to stand in the oven and subjected to secondary fermentation for about 60 days to obtain a secondary fermentation organism. During the second fermentation, the primary fermentation organic material was turned over at 5-day intervals to supply oxygen as a whole and prevent anaerobic fermentation. The secondary fermentation organic matter had a water content of about 35% and a total organic matter to nitrogen ratio (OM / N) of about 33. Thereafter, a mixture of secondary fermentation in the same amount of water and organic thickener in Bacillus Here Fu milreoseu (Bacillus pumilus), Bacillus subtilis (Bacillus subtilis), Bacillus Lee Kenny Po Ms (Bacillus licheniformis ) and Bacillus megaterium were added and kept under aerobic conditions for about 30 days and then concentrated. At this time, external air was supplied into the concentration tank through the bubble generator installed on the bottom surface of the thickener to maintain the aerobic condition. The concentrated fermentation organic matter was filtered by a sieve filter to remove the suspended matter, and then a coagulant (trade name: ECOMAX; manufactured by SENTI KOREA) was added thereto, and the fermentation organic matter was agglomerated into a cake state. Thereafter, the fermented organic material in a cake state was dried at room temperature to solidify the fermented organic material in a state of water content of 20% or less, and the solidified fermentation organic material was pulverized to obtain a fermented organic material powder.

25 parts by weight of ammonium sulfate, 15 parts by weight of ammonium phosphate and 9.5 parts by weight of potassium nitrate were added to and mixed with 32 parts by weight of the primary basic substance as a primary basic substance to prepare a secondary basic substance. Then, 2 parts by weight of borax, 0.5 part by weight of iron sulfate and 1 part by weight of ammonium molybdate were added to the secondary basic material and mixed to prepare a tertiary base material. Then, 10 parts by weight of a natural surfactant (a mixture of sodium cocoyl isethionate and a natural detergent for fruit washing), 2 parts by weight of potassium carbonate, 1.5 parts by weight of aluminum sulfate, 1 part by weight of gibberellin And 0.5 parts by weight of phosphoric acid were mixed to prepare a nutritional cleaner composition for removing soil contaminants in solid form.

2. Nutrition for removing soil pollutants Cleaner  Test for confirming the effect of calcium chloride removal in soil

A pot containing 250 g of acid soil was prepared for treatment. After 5 g of calcium chloride was placed on the soil surface of the remaining pot except the control (untreated), 25 ml of tap water per pot was sprayed with a sprayer so that the calcium chloride was completely dissolved and soaked in the soil. The pot was treated with calcium chloride and after 48 hours, the tap water, the solid phase cleaner composition prepared in Comparative Production Example 1 and the liquid cleaner composition prepared in Production Example 1 were sprayed according to the treatments. Table 1 below shows contents of processing for discrimination by treatment.

Treatment Processing contents Remarks No treatment Public soil Treated with calcium chloride Processing 1 Calcium chloride treated soil Treatment of calcium chloride 5g Treatment 2 Treated with calcium chloride + treated with 50 ml of tap water Treatment of calcium chloride 5g Treatment 3 Treated with calcium chloride + 50 ml of the solid phase cleaner composition of Comparative Preparation Example 1 50 ml of diluted solution Treatment of calcium chloride 5g Treatment 4 Treated with calcium chloride + 50 ml of liquid phase cleaner composition of Preparation Example 1 50 ml of diluted solution Treatment of calcium chloride 5g

Spraying the cleaner composition, etc., and after 48 hours, collected in the soil, each processing distinguished port and soil acidity (pH), chloride ion content (Cl ^-), a calcium ion content (Ca ^{2 +),} total nitrogen (TN), phosphoric acid (P ₂ O ₅ ) and potassium content (K ⁺ ) were measured. The results are shown in Table 2 below.

Treatment Acidity
(pH) Cl ^-
(%) Ca ^{2 +}
(cmol < ⁺ & gt ^; / kg) TN
(%) P ₂ O ₅
(Mg / kg) K ⁺
(cmol < ⁺ & gt ^; / kg) No treatment 5.38 0.008 3.42 0.060 43.8 0.22 Processing 1 5.63 0.147 17.24 0.072 40.9 0.25 Treatment 2 5.59 0.150 17.05 0.063 41.7 0.23 Treatment 3 6.10 0.038 5.47 0.121 133.5 0.65 Treatment 4 5.94 0.038 5.40 0.118 140.4 0.61

As shown in Table 2, the liquid cleaner composition prepared in Preparation Example 1 was superior in the calcium chloride removal effect to that of the tap water treatment, and was more excellent in the supply of phosphate ion than the solid cleaner composition prepared in Comparative Preparation Example 1 .

3. Nutrition for removing soil pollutants Cleaner  Test for determining the effect of the cleaning effect of the composition on the composition

A road neck was collected from the polluted plant. 20 g of the contaminated plants and 200 ml of each treatment solution were placed in an Erlenmeyer flask, shaken for 60 minutes and aged for about 24 hours to wash the contaminated plants. Table 3 below shows the treatments for discriminating the treatments.

Treatment Treatment liquid and dilution drainage Processing 1 No tap / dilution Treatment 2 Solid phase cleaner composition of Comparative Production Example 1/50-fold dilution Treatment 3 The liquid cleaner composition of Production Example 1/50-fold dilution

Then, the washed liquid was filtered with filter paper and the turbidity of the filtrate was compared. In addition, the pollutants remaining in the filter paper were dried at room temperature, and then the total weight of the filter paper and the pollutant was measured. 1 is a photograph showing turbidity of a filtrate obtained by treating a contaminated plant with tap water or a nutrient cleaner composition in an embodiment of the present invention. In Fig. 1, each image shows the tap water treatment area, the solid phase cleaner composition treatment of Comparative Production Example 1 and the liquid cleaner composition treatment of Production Example 1 in the order from left to right. Table 4 below shows the total turbidity of the treatments and the total weight of the filter paper and the contaminants. The larger the total weight of turbidity or combined filter paper and pollutant, the greater the tree cleaning ability.

Treatment Gross weight of filter paper and contaminant (g) Processing 1 2.29 Treatment 2 2.80 Treatment 3 2.82

As shown in FIG. 1 and Table 4, the liquid cleaner composition prepared in Preparation Example 1 was more effective than the tap water, and the same effect as that of the solid cleaner composition prepared in Comparative Example 1 was similar to that of the cleaner.

4. Nutrition for removing soil pollutants Cleaner  Improvement of plant growth and soil improvement effect of composition

The soil improvement effect of the nutrient cleaner composition for soil pollutant removal according to the present invention was indirectly evaluated through the influence of the effect on the growth of rapeseed.

Forty 800 pots with an inlet diameter of 6 ㎝ were filled with mountain soils, 20 seeds were sown on each pot, and 12 pots with uniform germination after 5 days were selected. The three sorted ports were treated as one treatment section, and the treatment for each of the treatments was carried out by using the solid phase cleaner composition of comparative preparation example 1 and the liquid phase preparation preparation example 1 of the comparative preparation example 1 for the biogrow-landscaping (soil remediation agent manufactured and sold by KARIM ENVIRONMENTAL DEVELOPMENT CO. The cleaner composition was treated and treated again after 5 days (total 2 treatments). Table 5 shows contents of treatment for discrimination.

Treatment Processing contents No treatment No processing Processing 1 A granular soil improvement agent (product name: Bio Gro - Landscape) was sprayed on the soil surface in an amount of 10 kg / m 2, then plowed and mixed with the upper soil layer Treatment 2 A 50-fold dilution of the solid phase cleaner composition of Comparative Preparation Example 1 was sprayed in an amount of 10 L / m < 2 > Treatment 5 A 50-fold dilution of the liquid cleaner composition of Preparation Example 1 was sprayed in an amount of 10 L / m < 2 >

After 20 days of sowing, the growth rate of rapeseed was measured. The rape growth rate was measured by separating the soil and oilseed rape from each treatment, washing the root part of the oil with water, removing the soil and foreign matter, drying it at room temperature and measuring the weight. FIG. 2 is a photograph showing the rapeseed growth rate after 20 days of sowing treated with commercially available soil remediation product or nutritional cleaner composition after rapeseed seeding in the embodiment of the present invention. In FIG. 2, each image shows an untreated area in the order from left to right, a "Biogro-landscape" treatment product, a commercially available soil remediation product, a solid phase cleaner composition treatment in Comparative Production Example 1, and a liquid cleaner composition treatment in Production Example 1. Table 6 shows the results of measurement of rape seedlings by treatments.

Treatment Rapeseed Growth (g) No treatment 1.04 Processing 1 1.55 Treatment 2 1.60 Treatment 3 1.82

As shown in FIG. 2 and Table 6, the liquid cleaner composition prepared in Preparation Example 1 was superior to the commercially available soil conditioner product or the solid phase cleaner composition prepared in Comparative Production Example 2, in terms of improving the growth of plants.

While the present invention has been particularly shown and described with reference to exemplary embodiments thereof, it is to be understood that the invention is not limited to the disclosed exemplary embodiments, but, on the contrary, is intended to cover various modifications and equivalent arrangements included within the spirit and scope of the appended claims. Therefore, the scope of the present invention should be construed as including all embodiments falling within the scope of the appended claims.

Claims (10)

Lactobacillus plantarum seedlings are inoculated in a liquid medium and cultured under anaerobic conditions to obtain a Lactobacillus plantarum culture; A nitrogen-containing substance, a potassium-containing substance, a phosphorus-containing substance, a trace element-containing substance necessary for plant growth, a surfactant, a plant growth regulator, a cationic neutralizer and an anionic neutralizer are added to and mixed with the lactobacillus plantarum A method comprising the steps of obtaining a final composition in liquid form,
Wherein the final composition in liquid form comprises 40-60 wt% of a Lactobacillus plantarum culture, 15-35 wt% of a nitrogen containing material, 6-20 wt% of a potassium containing material, 0.5-5 wt% of a phosphorus containing material 1 to 6 wt% of a trace element-containing substance necessary for plant growth, 2 to 8 wt% of a surfactant, 0.2 to 3 wt% of a plant growth regulator, 0.1 to 2 wt% of a cationic neutralizer and 0.1 to 2 wt of an anionic neutralizer % ≪ / RTI >
The liquid medium is prepared by mixing and dissolving a microorganism medium in water,
Wherein the microbial medium comprises molasses, glucose, sodium glutamate and yeast extract. ≪ RTI ID = 0.0 > 11. < / RTI >
The method of claim 1, wherein obtaining the final composition in liquid form
(a) adding a nitrogen-containing substance, a potassium-containing substance, and a phosphorus-containing substance to a lactobacillus plantarum culture liquid as a primary basic substance and mixing to prepare a secondary basic substance;
(b) adding a trace element-containing material necessary for plant growth to the secondary base material and mixing the same to prepare a tertiary base material; And
(c) adding a surfactant, a plant growth regulator, a cationic neutralizer, and an anionic neutralizer to the tertiary basement material and mixing the resulting mixture to prepare a final composition in the form of a liquid phase. A method for preparing a nutritional cleaner composition.
delete The method of claim 1 or 2, wherein the liquid medium is prepared by mixing and dissolving 1.5 to 3.5 parts by weight of a microbial medium per 100 parts by weight of water.
The microorganism culture according to claim 1 or 2, wherein the microorganism culture medium contains 40 to 60% by weight of molasses, 10 to 30% by weight of glucose, 5 to 15% by weight of sodium glutamate, 2 to 8% by weight of yeast extract, The method of claim 1, wherein the soil is contaminated with water.
The method according to claim 1 or 2, wherein the Lactobacillus plantarum seed is a solid phase seedlot having 10 ⁷ to 10 ⁹ Lactobacillus plantarum per unit g, 0.004 to 0.06 parts by weight based on the total weight of the composition.
The method according to claim 1 or 2, wherein the Lactobacillus plantarum has a Lactobacillus planta concentration of 10 ⁵ to 10 ⁸ cells / ml. .
3. The method according to claim 1 or 2,
The nitrogen-containing material is composed of at least one member selected from the group consisting of urea, ammonium sulfate, ammonium nitrate, ammonium chloride, ammonium phosphate, sodium nitrate and calcareous
The potassium-containing material is composed of at least one selected from the group consisting of potassium hydroxide, potassium sulfate, potassium nitrate, potassium chloride and potassium phosphate
The phosphorus-containing substance is composed of at least one member selected from the group consisting of phosphoric acid, potassium phosphate, ammonium phosphate, calcium phosphate, sodium phosphate and superphosphate
The trace element-containing material necessary for plant growth is selected from the group consisting of boron-containing material, zinc-containing material, iron-containing material, manganese-containing material, copper-containing material, calcium-containing material, molybdenum- Or more,
The surface active agent is _{[(CH 3) 3 SiO]} 3 Si (CH 2) 3 NH (CH 2) 3 NH (CH 2) 2 NH 2, C 6 H 6 (CH 3) 2 Si (CH 2) COOH, _{HOOCC 2 C 2 H 4 Si (} CH 3) 2 SiC 2 H 4 COOH, [(CH 3) SiO] 2 Si (CH 3) (CH 2) 3 OSO 3 H, lauryl amino propylene acid methyl, lauryl sulfate , Alkyl ether sulfonates, alkyl ether sulfonates, alkylamide sulfonates, alkane sulfonates, Igepon T (N-acyl-N-myrthyltaurin salts), fatty acid potassium, HO CH ₂ H ₄ O) SiC ₂ H ₄ COO (C ₂ H ₄ O) ₃ H, an alkali metal salt of a Coconut Oil Fatty Acid / Isethionic acid copolymer, sodium cocoyl isethionate Sodium Cocoyl Isethionate) and ammonium cocoyl isethionate, and more preferably at least one selected from the group consisting of ammonium cocoyl isethionate,
The plant growth regulator may be one or more selected from plant growth hormone,
The cationic neutralizing agent _{MgB 2, Mg (CO 3)} 2, NH 4 OH, (NH 4) 2 SO 4, (NH 4) 2 S 2 O 8, (NH 4) 2 CO 3, (NH 4) 3 _{PO 4, NH 4 HCO 3,} (NH 4) 2 CO 3 H 2 O, KBr, K 2 CO 3, K 3 [Co (NO 2) 6], Fe 3 O 4, Fe (CH 3 CO 2) 2 _{, K 2 SO 4, Fe (} OH) 3, FePO 4, Fe (C 6 H 11 O 7) 2, FeC 2 O 4, FeSO 4, Al 2 O 3 B 2 O 3, AlPO 4, Al (OH) ₃ , Al ₂ O ₃ and Al ₂ (SO ₄ ) ₃ ,
The anionic neutralizing agent is selected from the group consisting of H ₃ BO ₃ , H ₂ SO ₃ , H ₂ S ₂ O ₇ , H ₂ SO ₅ , H ₂ S ₂ O ₈ , C ₄ H ₈ SO ₂ , H ₂ SO ₄ , NH ₄ OH, (NH ₄ ) ₂ SO ₄ , K ₂ SO ₄ , Fe (OH) ₃ , FeSO ₄ , Al (OH) ₃ and Al ₂ (SO ₄ ) ₃ . Wherein the method comprises the steps of:
9. The method of claim 8,
Wherein the nitrogen-containing material is an element,
Wherein the potassium containing material is potassium secondary phosphate,
Wherein the phosphorus containing material is phosphoric acid,
The trace element-containing material necessary for plant growth is composed of borax, zinc sulfate and ammonium molybdate,
The surfactant is composed of sodium salt of coconut oil fatty acid / isethionic acid copolymer and sodium cocoyl isethionate,
Wherein the plant growth regulator is gibberellin,
The cationic neutralizing agent is K ₂ CO ₃ ,
Wherein the anionic neutralizing agent is H ₃ BO ₃ .
The composition of claim 9, wherein the final composition in liquid form comprises from 40 to 60% by weight of the Lactobacillus plantarum culture, from 15 to 35% by weight of urea, from 6 to 20% by weight of potassium phosphate dibasic, 0.5 to 5 wt% of borax, 0.1 to 2 wt% of zinc sulfate, 0.1 to 2 wt% of ammonium molybdate, 2 to 8 wt% of a surfactant, 0.2 to 3 wt% of a plant growth regulator, 0.1 to 2% by weight of an anionic neutralizing agent, and 0.1 to 2% by weight of an anionic neutralizing agent.

Images