KR101647135B1

KR101647135B1 - Soil conditioner manufactured by using complex microbe spawn and method for manufacturing the same

Info

Publication number: KR101647135B1
Application number: KR1020150067632A
Authority: KR
Inventors: 김광원; 최영재
Original assignee: 주식회사 기술과창조; 주식회사 씨드바이오
Priority date: 2015-05-14
Filing date: 2015-05-14
Publication date: 2016-08-09
Also published as: WO2016182364A1

Abstract

(Bacillus subtilis) , Lactobacillus plantarum (Lactobacillus plantarum ) , Lactobacillus plantarum (Lactobacillus plantarum ) , Lactobacillus plantarum (Lactobacillus plantarum ) , and Lactobacillus plantarum using a culture medium prepared by mixing lysine, propolis, molasses, Plantarum , Saccharomyces cerevisiae, and the like are cultured, and the liquid material having the pH value of the cultured liquid material measured within a predetermined range is filtered to complete the soil remediation agent do.

Description

[0001] The present invention relates to a soil improvement agent prepared by using a complex microbial strain and a method for producing the same,

The present invention relates to a method for producing a soil improving agent using a seed microorganism having a multiplicity of microorganisms and a soil improving agent prepared by the method.

Soil is a substance deposited on the surface of the earth. Most of the soil is weathered rocky weathered rocks exposed to the surface or near the surface of the earth are exposed to oxygen, water and heat, , And organic matter. In agriculture and forestry, soil is defined as nutrients, moisture storage and regulation, release, and support of plants in plants.

Soil pollution occurs when various harmful substances produced by human activities are absorbed into soil and soil is lost as an environmental component. Soil contamination is generally caused by the use of underground resources, It is generated by accumulation of synthetic organic chlorine compound or alkyl mercury compound by pesticide, and it is also caused by acid rain by industrial complex and city gas, waste of food packaging, waste of facility livestock. In addition, inorganic elements such as heavy metals released by industrialization pollute farmland soil and cause crop damage, causing harm to people and livestock during the food chain. One of the alternatives used for solving such soil contamination is a soil improvement agent. The soil improvement agent is used to improve the physical, chemical and biological properties of the soil and to improve physical properties by aggregating or joining the soil. It is also called disinfectant.

On the other hand, in the prior art disclosed in Korean Patent No. 10-1259416, 0.001 to 0.02 parts by weight of a mixed microorganism (BM-S-1) of deposit number KCTC 11789BP, 2 to 5 parts by weight of fermented rice bran, 3 to 7 parts by weight Water is mixed with 5 to 10 parts by weight of a shiitake mushroom powder, 3 to 5 parts by weight of peatmoss powder, 1 to 3 parts by weight of molasses, 2 to 5 parts by weight of trehalose, and 0.5 to 2 parts by weight of bamboo activated charcoal powder, And the mixed microorganism solution is mixed with a culture raw material to prepare a mixed raw material, and the microorganism is inoculated into the mixed raw material and cultured to prepare a soil improving agent. This prior art has a disadvantage in that the process for producing mixed raw materials is complicated and includes a high-temperature inoculation step, a drying step, and a molding step, which complicates the process and lengthens the manufacturing time.

As described above, although the soil improvement agent produced using microorganisms such as the prior art disclosed in the above-mentioned patent is complicated and the production time is prolonged, the number of beneficial bacteria included in the soil improvement agent is not sufficient, .

Friendly soil improving agent which can increase the number of beneficial bacteria in a short time through a simple manufacturing process. The present invention also provides a soil conditioner produced by the method.

According to one aspect of the present invention, there is provided a method for producing a soil conditioner using a complex microorganism strain, which comprises cultivating Bacillus subtilis , Lactobacillus plantarum , and Saccharomyces cerevisiae Propolis, molasses, and water in a culture vessel together with a seed microorganism containing a plurality of microorganisms including the microorganism, and a culture liquid formed by mixing lysine, propolis, molasses, and water introduced into the culture vessel A culture step of culturing the complex microorganism seed introduced into the culture vessel, a measurement step of measuring the pH value of the liquid material cultured in the culture step, and a step of measuring the pH value of the soil by filtering the liquid material measured in a predetermined range of the pH value And a filtration step to complete the modifying agent.

The complex microorganism strain is selected from the group consisting of Lactobacillus casei , Lactobacillus acidophilus , Lactobacillus leuconostoc , Lactobacillus brevis , Streptococcus faecalis, the present invention may further include at least one of faecalis , Bacillus putrificus , Bacillus cereus , Pseudomonas fluorescens , and Aspergillus oryzae . The complex microorganism strain may further include at least one of green sulfur bacteria, red sulfur bacteria, and red non-sulfur bacteria.

The step of introducing may include 0.7 to 0.9 parts by weight of a complex microbial strain, 0.1 to 0.3 parts by weight of lysine, 0.1 to 0.3 parts by weight of propolis and 4.5 to 6.5 parts by weight of molasses in relation to 100 parts by weight of water, Wherein the culture medium is prepared by mixing 0.1 to 0.3 parts by weight of lysine, 0.1 to 0.3 parts by weight of propolis and 4.5 to 6.5 parts by weight of molasses per 100 parts by weight of the combined microorganism strain Can be cultured.

The culture step is carried out by aeration while simultaneously intermittently stirring 0.7 to 0.9 part by weight of the complex microbial species, 0.1 to 0.3 part by weight of lysine, 0.1 to 0.3 part by weight of propolis and 4.5 to 6.5 parts by weight of molasses, The complex microorganism seeds can be cultured. The culturing step may be performed by repeating the stirring and the aeration for a second time longer than the first time after the stirring and the aeration for the first time are repeated to perform the intermittent stirring and aeration. In the culturing step, the intermittent stirring and aerating may be performed while the temperature of the culture liquid is maintained within a range of 35 to 45 ° C.

The filtering step may complete the soil conditioner by filtering the liquid material measured in the pH range of 3 to 4.5 in the measuring step. The liquid phase material corresponding to the soil conditioner may be discharged from the filter by filtering the liquid phase material having a pH value measured within a predetermined range in the measuring step using at least one filter having a pore size of 200 mesh have.

Another aspect of the present invention is to provide a soil amendment agent by the method for preparing a soil amendment agent using the complex microorganism.

(Bacillus subtilis) , Lactobacillus plantarum , and Saccharomyces cerevisiae using a culture medium in which lysine, propolis, molasses and water are mixed. By cultivating a seed microorganism having a microorganism complex, it is possible to produce a soil amendment agent in which the number of microorganisms is greatly increased.

A mixed microorganism having 0.7 to 0.9 part by weight based on 100 parts by weight of water is mixed with 100 parts by weight of water using 0.1 to 0.3 parts by weight of lysine, 0.1 to 0.3 parts by weight of propolis and 4.5 to 6.5 parts by weight of molasses. By cultivating seed bacterium, a soil amendment agent with a greatly increased number of microorganisms can be produced in a short time.

The soil amendment agent according to the present invention can be produced by a simple process consisting of the purification step 10, the input step 20, the cultivation step 30, the measurement step 40, and the filtration step 50 and the like. In addition, since any process in the process of producing the soil conditioner does not require a chemical substance, an environmentally friendly soil conditioner can be provided.

1 is a flow chart of a method for producing a soil conditioner according to an embodiment of the present invention.

Hereinafter, the contents of the present invention will be described in more detail.

The present invention relates to a method for producing Bacillus subtilis, Lactobacillus plantarum, and Sakae using a culture medium containing lysine, propolis, molasses, and water. The present invention relates to a soil remediation agent prepared by culturing a seed microorganism having a multiplicity of microorganisms, including Saccharomyces cerevisiae, and filtering the cultured liquid material.

The soil conditioner according to the present invention is prepared by mixing 0.7 to 0.9 part by weight of a complex microbial strain, 0.1 to 0.3 part by weight of lysine, 0.1 to 0.3 part by weight of propolis and 4.5 to 6.5 parts by weight of molasses, based on 100 parts by weight of water, Is incubated with intermittent agitation and aeration while maintaining the temperature in the range of 35 to 45 ° C.

Bacillus subtilis, Lactobacillus plantarum and Saccharomyces cerevisiae, which are used in the production of soil conditioner, have been found to be useful for the production of soil microorganisms, , Molasses, and the nature and use of water will be described later.

Bacillus Bacillus subtilis is called Bacillus subtilis (a species of aerobic bacteria that is not toxic and forms spores). It is a type of bacterium belonging to the genus Bacillus, which is widely distributed in the natural world such as soil, hay, and dust, and is an aerobic microorganism. Bacillus subtilis produces a large amount of beneficial enzymes and biologically active substances through metabolic activities and supplies them to microorganisms in the soil and plants planted in the soil to support their growth. In addition, various antibiotics are secreted to prevent various insects caused by plants.

Lactobacillus plantarum is a salt tolerant lactic acid bacterium (a bacterium that produces lactic acid by decomposing saccharides such as glucose), and corresponds to flowable anaerobes. Lactobacillus plantarum is a byproduct that produces organic acids, inhibits the growth of other bacteria, improves the soil, and produces lactolin, one of the outstanding antimicrobials, to prevent insect pests.

Saccharomyces cerevisiae (Saccharomyces cerevisiae) is a typical yeast belonging to the carrot fungus (bacteria that form adenocarcinoma by the sexual reproduction in the fungus and produces the ascospores), and most of the yeast used for brewing Can be said to be close to this species. Saccharomyces cerevisiae provides a useful bioactive substance such as amino acids, vitamins, minerals, and digestive enzymes through metabolism to promote the activity of soil microorganisms, and through the fermentation, decomposition of organic matter using microorganisms To improve the soil by promoting the activity of soil microorganisms.

The complex microorganism strain of the present invention can be obtained from soybean, molasses, rice and the like. In addition to the above-mentioned Bacillus subtilis, Lactobacillus plantarum, and Saccharomyces cerevisiae , Lactobacillus casei, Lactobacillus acidophilus, Lactobacillus leuconostoc, Lactobacillus brevis, Streptococcus faecalis , Bacillus subtilis, Lactobacillus spp., Lactobacillus spp., Lactobacillus spp., Lactobacillus spp. Such as Bacillus putrificus, Bacillus cereus, Pseudomonas fluorescens, Aspergillus oryzae, and the like.

In addition, the complex microorganism strain of the present invention may further include photosynthetic bacteria such as green sulfur bacteria, red sulfur bacteria, and red non-sulfur bacteria in addition to the above-mentioned microorganisms. Photosynthetic bacteria are photosynthetic bacteria that use light energy to carry out carbon assimilation. Bacteriocholorophyll uses photosynthetic carbon dioxide and hydrogen compounds instead of chlorophyll. Bacteriocin Chlorophyll is a pigment of photosynthetic bacteria. Several chromophores such as a and b exist, and the definite main chromophoric structure is called bacteriol chlorophyll a. Its structure is chlorophyll a (C ₅₅ H ₇₂ MgN ₄ O ₅ ) (C ₅₅ H ₇₄ MgN ₄ O ₅ ). These bacteriophilic chlorophylls exhibit a major absorption peak at about 357.5 nanometers and about 770 nanometers in an alcohol solution and are associated with proteins in the cell and exhibit absorption maxima at about 380, 800, 850, 870 and 890 nanometers, A light portion of 890 nanometers is used for photosynthesis. In particular, in the case of scarlet non-sulfur bacteria, low-level fatty acids from organic pollutants are used as photosynthetic materials, and they grow well in both anaerobic and aerobic conditions.

These photosynthetic bacteria are added to the soil improving agent prepared according to the present invention to improve the soil by acting on carbon assimilation, nitrogen fixation, odor removal and the like. The above-mentioned carbon assimilation refers to the action of any bacterium to produce an organic carbon compound with carbon dioxide and water. The organic carbon compound generated by the carbon assimilation is supplied to the plant, and the supplied organic carbon compound is used as the energy source of the plant, To help plant growth. Photosynthetic bacteria supply nitrogen to plants planted in soil and soil with excellent nitrogen fixation ability, and produce excellent antimicrobial and antiviral substances through metabolism to inhibit the growth of harmful bacteria, and hydrogen sulfide , Ammonia, acetic acid, and amine-based substances to remove odors. In addition, since harmful substances are removed and the growth of harmful microorganisms is suppressed, the activity of other microorganisms present in the soil amendment agent is enhanced.

However, since the photosynthetic bacteria are highly resistant to bacterial cell wall destruction, they can interfere with the growth of beneficial bacteria as well as harmful bacteria, and they have the problem of increasing sulfides in the soil by performing sulfation which generates sulfates or esters Be careful not to use too much. Generally, each microorganism strain is mixed with 1 to 40% by weight of each microorganism strain, and the number of each microorganism strain is about 10 ² to 10 ³ cfu / g.

If the amount of the microorganism of the present invention is less than 0.7 parts by weight based on 100 parts by weight of water, the microorganisms necessary for cultivation are not sufficiently supplied. Therefore, the growth of the microorganism population through the cultivation does not occur effectively, The proportion of microorganisms is higher than that of lysine, propolis, molasses, and water used for the culture of microorganisms, so that the microorganisms may become difficult to multiply through sufficient feeding activity As the medium contained in the complex microorganism strain is mixed excessively, contamination of the culture liquid by the by-products may occur.

Lysine is a basic amino acid that acts as a growth promoter that promotes growth and development and is also called lysine. In the present invention, lysine is used as a culture food of a complex microorganism strain such as Bacillus subtilis, Lactobacillus plantarum, Saccharomyces cerevisiae, etc., To increase the number of beneficial bacteria. Some of the remaining lysine, which is used as a feed for the culture of complex microorganisms, remains as a soil remediation agent and can help decompose dioxin, a harmful substance present in the soil. In the case of L-lysine, one of the isomers of lysine It increases the resistance to disease and helps to prevent pests of plants.

When the amount of lysine used in the present invention is less than 0.1 part by weight per 100 parts by weight of water, the microbial proliferation efficiency of the microbial organisms may not be sufficiently supplied to the food of the complex microorganism in the cultivation step, It may be difficult to remove harmful substances from the soil, and when the amount of lysine is more than 0.3 part by weight, the culture and microbial culture space in the culture liquid may be insufficient and the efficiency of propagation of microorganisms may be decreased.

Propolis is a substance made by mixing bees with their own needles and enzymes in the same material as resin (resin) extracted from various plants for their survival and propagation. Propolis is composed of organic matter and minerals Many minerals, vitamins, amino acids, fats, organic acids and flavonoids play an important role in cell metabolism and terpenes have anticancer activities. In the present invention, it is used as a feed for the seed of the complex microorganism in the culturing step to help increase the number of beneficial bacteria. Some of the remaining propolis, which is used as a feed for cultivation of complex microorganisms, remains as a soil remediation agent and suppresses the growth of harmful microorganisms in the soil, and acts as an environmentally friendly disinfectant with excellent sterilizing power and antibacterial power.

When the propolis is used in an amount of 0.1 to 0.3 parts by weight based on 100 parts by weight of water, the propagation efficiency of the microorganisms may be lowered due to insufficient feeding of the complex microorganisms in the culture step, If the amount of propolis is more than 0.3 part by weight, the culture and microbial growth of the microorganism in the culture medium may be insufficient and the propagation efficiency of the microorganisms may be decreased. Also, due to the excessive antibacterial activity, Growing of useful bacteria may also be difficult.

Molasses is a black syrup-like liquid that is extracted from sugars during the process of sugar production through sugarcane, sugar beet, etc., through the processes of dissolution, purification and crystallization. It contains 20 ~ 30% moisture The main ingredient is sugar. The molasses is divided into sugar cane molasses, sugar beet molasses, cornstarch molasses, citrus molasses, molasses, and molasses according to the raw materials. Molasses is a substance having various nutrients such as dietary fiber, folic acid, protein, vitamin, and potassium. In the present invention, the molasses is used as a culture food for a complex microorganism strain.

The molasses is a liquid viscous substance having a viscosity of 500 Pa · s and a viscosity higher than 1 mPa · s (20 ° C) of water. Therefore, in the present invention, molasses is melted separately at 40 ° C. and put into a culture vessel. In addition, although glucose and brown sugar can be used as the fermented food of the complex microorganism strain instead of molasses, the cost saving effect is obtained by using molasses which is relatively easy to obtain and low in cost.

When the molasses used in the present invention is 4.5 to 6.5 parts by weight based on 100 parts by weight of water and less than 4.5 parts by weight. In the culture stage, the multiplication efficiency of the microorganisms may be lowered due to insufficient supply of the culture food of the complex microbial strain, and the molasses exceeds 6.5 parts by weight. The culture efficiency of the microorganisms may be lowered due to insufficient microbial culture and space in the culture medium, and when the molasses having high viscosity is used excessively, the viscosity of the culture medium becomes excessively high, so that it becomes difficult to homogeneously mix the microorganisms with the culture medium. The proliferation efficiency of microorganism seedlings may be decreased.

In the present invention, all components other than the complex microbial strain, lysine, propolis, and molasses are all composed of water, and water is used as a solvent for the raw materials. Water has a specific heat that is large enough to control the body temperature of a thermophilic animal, so it is not sensitive to changes in temperature, such as temperature, and does not cause a chemical reaction with other substances, so it is suitable for use as a solvent.

The soil conditioner according to the present invention is prepared by mixing 0.7 to 0.9 parts by weight of a complex microbial strain, 0.1 to 0.3 parts by weight of lysine, 0.1 to 0.3 parts by weight of propolis, and 4.5 to 6.5 parts by weight of molasses, based on 100 parts by weight of water, The mixing ratio of one ingredient is an optimal condition for culturing the multiple microorganism strain, and a preferred embodiment will be described below.

Hereinafter, a method for producing a soil conditioner using a multiple microbial strain according to an embodiment of the present invention will be described in detail with reference to the drawings.

1 is a flow chart of a method for producing a soil conditioner according to an embodiment of the present invention. Referring to FIG. 1, a method for producing a soil conditioner according to the present embodiment comprises an purification step 10, an input step 20, a cultivation step 30, a measurement step 40, and a filtration step 50 .

In the water purification step (10), tap water is purified to remove by-products such as chlorine from tap water. More specifically, in the water purification step 10, tap water can be poured into a heating container provided separately from the culture container and purified by heating for 3 to 24 hours in a temperature range of 60 to 80 ° C. Tap water contains chlorine (Cl), fluorine (F), calcium (Ca), magnesium (Mg), iron (Fe), other byproducts and mixed germs other than certain beneficial bacteria. In the water purification step (10), tap water is purified by water suitable for the growth of microorganisms by removing such chlorine, bacteria, and other by-products from tap water.

In the injecting step 20 , a seed microorganism having a multiplicity of microorganisms including Bacillus subtilis, Lactobacillus plantarum and Saccharomyces cerevisiae is mixed with lysine, propolis, , Molasses, and water are put into a culture container. According to the present embodiment, 0.7 to 0.9 parts by weight of complex microbial species, 0.1 to 0.3 parts by weight of lysine, 0.1 to 0.3 parts by weight of propolis and 4.5 to 6.5 parts by weight of molasses are added to 100 parts by weight of water . This input step 20 follows the following series of steps. First, water purified in the water purification step (10) is cooled and water corresponding to about 80% of the total amount of water to be added to the culture vessel is introduced into the culture vessel when the temperature of the water reaches about 40 ° C which is the optimum culture temperature of the microorganism .

Molasses dissolved at about 40 占 폚 is then introduced into a culture vessel, and then the complex microorganism strain, lysine, and propolis are put into a culture vessel. The reason why the molasses is first added to the mixed microorganism seedlings, lysine, and propolis is that the molasses is highly viscous, and when it is added later than the complex microbial strain, lysine, and propolis, This is because mixing can be disturbed. Lastly, about 20% of the total amount of water to be fed into the culture container is put into the culture container until the space corresponding to about 85% of the culture container is filled, and then the lid of the culture container is closed. To ensure a smooth supply of oxygen to the culture medium containing lysine, propolis, molasses, and water, approximately 15% of the culture space should be empty. Water is injected into the culture vessel in two portions as described above in order to precisely empty space corresponding to about 15% of the culture container.

In the incubation step 30, Bacillus subtilis , Lactobacillus plantarum , and Saccharomyces cerevisiae , which are introduced into a culture container using a culture medium containing lysine, propolis, molasses, and water, Saccharomyces cerevisiae is cultivated in the seed microbes. In the prior art, microorganisms are cultured mainly by feeding molasses. As shown in Test Example 2 to be described later, when the lysine, propolis and molasses are added together, the number of microorganisms is greatly increased compared with the case where only the molasses is injected. According to this example, only by incubating the complex microorganism strain as described above for about 72 hours, the population of the microorganism was sharply increased as shown in Test Example 1 to be described later.

In the incubation step 30, 0.7 to 0.9 part by weight of complex microorganism seeds, 0.1 to 0.3 part by weight of lysine, 0.1 to 0.3 part by weight of propolis, and 4.5 to 4.5 parts by weight of molasses are added to 100 parts by weight of water, And 6.5 parts by weight of water are simultaneously stirred while being aerated to cultivate the complex microorganism strain. As shown in Test Example 1 to be described later, when the complex microorganism strain is cultured in the composition ratio as described above, the microbial population is explosively increased in a short time. In particular, for explosive growth of these microbial populations, agitation and aeration must be carried out at appropriate intervals. Agitation of the culture liquid may be carried out by means of a device for wetting the culture liquid, and the aeration of the culture liquid may be carried out by an air pump or the like provided inside the culture vessel.

If the agitation time of the culture medium is too long, the microorganisms can not take a sufficient rest and the increase in the number of microorganisms is slow. If the agitation time of the culture medium is too short, the food is concentrated in the culture medium and the increase in the number of microorganisms becomes slow. If the aeration time of the culture medium is excessively long, anaerobic microorganisms that do not require oxygen are killed and the growth of the microbial population is slow. If the aeration time of the culture medium is too short, the aerobic microorganisms requiring oxygen are killed and the increase of microbial population is delayed.

In the incubation step 30 according to the present embodiment, in order to explosively increase the number of microorganisms for the reasons described above, the culturing step is a step of stirring and aeration for about 2 hours and then stopping stirring and aeration for about 4 hours Intermittent stirring and aeration are carried out by repeating. The reason why the time to stop stirring and aeration is twice longer than the time to stir and aeration is because the microbes need more time to rest after metabolic activity than the time they eat food, .

In the measuring step 40, the pH of the liquid material cultured in the culturing step 30 is measured using a pH meter. The pH of the liquid material may be measured while the pH meter is immersed in the liquid material cultured in the culturing step 30. Alternatively, a small amount of the liquid material cultured in the culturing step 30 may be collected and measured by a pH meter. When the measured pH value is between 3 and 4.5, the cultured liquid material is filtered and used as a soil conditioner. The cultured liquid material is characterized by having a herbaceous and sour taste. On the other hand, if the measured pH value of the liquid material cultured in the cultivation step 30 is not between 3 and 4.5, the cultured liquid material is discarded.

In the filtration step (50), the soil improvement agent is completed by filtering the liquid matter measured in the predetermined range of the pH value in the measuring step (40). Here, the predetermined range is preferably 3 to 4.5. For example, the filtration step 50 may filter the liquid material measured at a pH value of 3-4.5 in the measuring step 40 with a filter having a porosity of 200 mesh size. A filter with a mesh size of 200 mesh can be made by drilling several holes of 200 mesh size on the bottom of a container made of acrylic material. When a liquid substance whose pH value is measured within 3 to 4.5 is put into such a container type filter, the liquid substance discharged from the filter becomes a soil conditioner.

In the measurement step 40, the liquid substance measured at a pH value within the range of 3 to 4.5 includes Bacillus subtilis , Lactobacillus plantarum , Saccharomyces cerevisiae ), Lysine and propolis, as well as molasses and residual by-products remaining in the seeds. The molasses in the liquid material has an easily rotting property, so that when a large amount of molasses is contained in the liquid material, the liquid material is deteriorated during long-term storage of the liquid material. Likewise, residual by-products in the liquid material alter the liquid material during the long-term storage of the liquid material.

Therefore, molasses and residual by-products in the liquid material measured at a pH value within the range of 3 to 4.5 should be removed as this will deteriorate the soil conditioner. The aforementioned complex microbial species, lysine, and propolis have the property of passing through a hole of 200 mesh size, while molasses and the remaining byproduct do not pass through a hole of 200 mesh size. Thus, molasses and remaining by-products in the liquid material can be removed by filtering the liquid material measured at a pH value of 3 to 4.5 by the above-described method. As such, the soil remediation agent after the filtration step 50 does not contain molasses and residual byproducts, so that the deterioration of the soil remediation agent is prevented and the state of the soil remediation agent can be preserved for a long period of time. For example, when soil remediation was stored for 6 months, not only did not rot but also the number of beneficial bacteria was almost unchanged.

On the other hand, the filtration process as described above can be repeated several times in order to reliably remove unnecessary components and by-products from the liquid substance whose pH value is measured within 3 to 4.5. For example, four filters with pores of 200 mesh size can be prepared and the liquid material measured at pH values between 3 and 4.5 can be passed through the four filters in turn. That is, the liquid material filtered by any one of the filters is passed to the next filter to be filtered. The liquid material passing through the fourth filter corresponds to the soil improvement agent and is put in a storage container and released to the market.

The soil conditioner according to the present embodiment is a simple process comprising a purification step 10, an input step 20, a cultivation step 30, a measurement step 40, and a filtration step 50 according to the above- . &Lt; / RTI > In addition, since any process in the process of producing the soil conditioner does not require a chemical substance, an environmentally friendly soil conditioner can be provided. On the other hand, the soil improvement agent is a diluted solution prepared by mixing a certain amount of water and a soil conditioner, and a diluted solution obtained by mixing a certain amount of water and a soil conditioner can be sprayed on the soil or sprayed on plants.

In addition, the soil conditioner prepared according to the above-mentioned steps is sprayed on the soil to remove various pollutants from the soil to purify the soil, and provides beneficial components generated by the activities of various microorganisms to plants planted in the soil and the soil, .

Hereinafter, the soil amendment agent using the complex microbial strain of the present invention will be described in detail through examples, comparative examples and test examples. These embodiments are only for illustrating the present invention, and thus the scope of the present invention is not construed as being limited by these embodiments.

&Lt; Example 1 >

According to the embodiment of the present invention described above, a soil conditioner was prepared as follows. The contents of the embodiments described above are followed even if omitted. 1000 Kg of tap water was put in a heating container, and the mixture was heated to 80 캜 and maintained for 12 hours. Subsequently, while the water was slowly cooled, when the water temperature reached 40 DEG C, 800 Kg of water was added to the culture container. Then, 50 kg of molasses, 7.5 kg of complex microbial strain, 1.5 kg of lysine and 1.5 kg of propolis were added to the culture container. Here, the complex microorganism strain was prepared by mixing 40% by weight of Bacillus subtilis, 30% by weight of Lactobacillus plantarum , and 30% by weight of Saccharomyces cerevisiae .

Subsequently, 200 kg of water remaining in the culture vessel was added. Subsequently, the culture medium in the culture vessel was stirred and aerated for 2 hours, followed by stirring and aeration for 4 hours. The culture was repeated for a total of 72 hours, and the temperature of the culture medium was maintained at 40 占 폚. Subsequently, the liquid material having a pH value of 4 was filtered four times using a filter having a pore size of 200 mesh, and the liquid material having passed through the fourth filter was placed in a storage container to complete the preparation of the soil conditioner.

&Lt; Example 2 >

According to the embodiment of the present invention described above, a soil conditioner was prepared as follows. The contents of the embodiments described above are followed even if omitted. 1000 Kg of tap water was put in a heating container, and the mixture was heated to 80 캜 and maintained for 12 hours. Subsequently, while the water was slowly cooled, when the water temperature reached 40 DEG C, 800 Kg of water was added to the culture container. Then, 50 kg of molasses, 7.5 kg of complex microbial strain, 1.5 kg of lysine and 1.5 kg of propolis were added to the culture container. Herein, the complex microorganism strain is composed of 35% by weight of Bacillus subtilis, 30% by weight of Lactobacillus plantarum, 30% by weight of Saccharomyces cerevisiae, 5% by weight.

&Lt; Comparative Example 1 &

According to the embodiment of the present invention described above, a soil conditioner was prepared as follows. The contents of the embodiments described above are followed even if omitted. 1000 Kg of tap water was put in a heating container, and the mixture was heated to 80 캜 and maintained for 12 hours. Subsequently, while the water was slowly cooled, when the water temperature reached 40 DEG C, 800 Kg of water was added to the culture container. Then, 50 kg of molasses and 7.5 kg of mixed microbial strain were added to the culture container. Subsequently, 200 kg of water remaining in the culture vessel was added. Here, the complex microorganism strain was prepared by mixing 40% by weight of Bacillus subtilis, 30% by weight of Lactobacillus plantarum , and 30% by weight of Saccharomyces cerevisiae .

Subsequently, the culture medium in the culture vessel was stirred and aerated for 2 hours, followed by stirring and aeration for 4 hours. The culture was repeated for a total of 72 hours, and the temperature of the culture medium was maintained at 40 占 폚. Subsequently, the liquid material having a pH value of 4 was filtered four times using a filter having a pore size of 200 mesh, and the liquid material having passed through the fourth filter was placed in a storage container to complete the preparation of the soil conditioner.

&Lt; Comparative Example 2 &

According to the embodiment of the present invention described above, a soil conditioner was prepared as follows. The contents of the embodiments described above are followed even if omitted. 1000 Kg of tap water was put in a heating container, and the mixture was heated to 80 캜 and maintained for 12 hours. Subsequently, while the water was slowly cooled, when the water temperature reached 40 DEG C, 800 Kg of water was added to the culture container. Then, 50 kg of molasses, 7.5 kg of mixed microbial species, 3.5 kg of lysine and 4 kg of propolis were added to the culture container. Subsequently, 200 kg of water remaining in the culture vessel was added. Here, the complex microorganism strain was prepared by mixing 40% by weight of Bacillus subtilis, 30% by weight of Lactobacillus plantarum , and 30% by weight of Saccharomyces cerevisiae .

&Lt; Test Example 1 >

The results shown in Table 1 below are the results of tests conducted by Chungnam National University Agricultural Science and Technology Center located in Daejeon City, Chungcheongnam-do, Korea to confirm the superiority as a soil conditioner through the microorganism test according to the embodiment of the present invention.

Inspection items unit test results Bacillus subtilis cfu / g 3.0 × 10 ⁹ Lactobacillus plantarum cfu / g 2.0 × 10 ¹⁰ Saccharomyces cerevisiae cfu / g 7.0 × 10 ⁹

Table 1 shows test results for Bacillus subtilis, Lactobacillus plantarum and Saccharomyces cerevisiae microbial populations of the soil conditioner prepared according to Example 1.

When Bacillus subtilis, Lactobacillus plantarum, and Lactobacillus plantarum were cultured in the presence of lysine, propolis, and molasses at the same time as the feed of the complex microorganism strain according to Example 1 of the present invention, At least 3.0 x 10 ⁹ microorganisms were detected in Saccharomyces cerevisiae .

&Lt; Test Example 2 &

Table 2 shows test results for Bacillus subtilis, Lactobacillus plantarum, and Saccharomyces cerevisiae microbial populations of the soil conditioner prepared according to Comparative Example 1.

Inspection items unit test results Bacillus subtilis cfu / g 5.0 × 10 ⁶ Lactobacillus plantarum cfu / g 4.0 × 10 ⁶ Saccharomyces cerevisiae cfu / g 1.5 x 10 ⁷

In Comparative Example 1, molasses was cultured as a microbial feed, and the complex microbial strain was cultured as in the prior art. Comparison of Table 1 and Table 2 showed that the number of microorganisms was greatly increased when lysine, propolis and molasses were added together, compared to the case where only molasses was added. Therefore, it can be seen that the addition of lysine, propolis, and molasses at the same time to the culture feed of the microorganism is effective for increasing the number of microorganisms.

&Lt; Test Example 3 >

Inspection items unit test results Bacillus subtilis cfu / g 4.0 × 10 ⁷ Lactobacillus plantarum cfu / g 6.0 × 10 ⁸ Saccharomyces cerevisiae cfu / g 4.0 x 10 ⁸

Table 3 shows the test results of Bacillus subtilis, Lactobacillus plantarum and Saccharomyces cerevisiae microbial populations of the soil conditioner prepared according to Comparative Example 2.

In the case of Comparative Example 2, the complex microorganism strain was cultured by feeding lysine, propolis and molasses at the same time as the food of the complex microorganism strain. However, the amount of lysine and propolis was excessively added Lt; / RTI > As a result, it can be seen that the numbers of microorganisms of Bacillus subtilis, Lactobacillus plantarum and Saccharomyces cerevisiae are decreased as compared with those of Table 1. [

The following tests were conducted using lettuce to determine whether the soil conditioner prepared according to Example 1 and the soil conditioner prepared according to Example 2 were suitable for crop growth.

First, for 28 days, seedlings (a young seedling or a nursery used as a breeding crop for growing crops) were formally transferred to a field that was cultivated in a hill or a seedbed. In the experimental group 1, the soil conditioner prepared according to Example 1 was diluted 1000 times with water and sprayed on the soil. In the experimental group 2, the soil conditioner prepared according to Example 2 was diluted 1000 times with water and sprayed on the soil. , No treatment was performed for the control group. Table 4 shows the plant height (the length from the surface to the tip of the herbaceous plant) and number of leaves (number of plant leaves) of each lettuce in the initial lettuce of each group.

Control group Experiment 1 Experiment 2 Length (cm) 8.25 8.42 8.48 Number of leaves (sheets) 9.5 9.8 9.8

Then, in the experimental group 1, the soil conditioner prepared according to Example 1 was diluted 1000 times with water and sprayed on the soil. In the experimental group 2, the soil conditioner prepared according to Example 2 was diluted 1000 times and sprayed. Each experimental group was sprayed with soil remediation agent every 5 days, and other cultivation management was complied with RDA standard farming manual.

The growth condition of lettuce after 10 days from the date of planting was firstly investigated. The soil improvement agent was sprayed twice in the experimental group 1 and the experimental group 2 for 10 days, and the results are shown in Table 5 below. As shown in Table 5, it can be seen that the growth efficiency of lettuce was higher in the experimental group 1 and the experimental group 2 in which the soil amendment agent prepared in Example 1 and Example 2 were sprayed than the control group.

The first survey (10 days after the ceremony) Control group Experiment 1 Experiment 2 Length (cm) 12.11 12.88 13.44 Number of leaves (sheets) 9.5 10.7 11

In the case of leaf number, about 1 leaf was more in the experimental group 1 and 1.5 sheets in the experimental group 2, compared with the control group. In the plant height, the control group was about 3.86 cm longer, whereas the experimental group 1 was about 4.46 cm longer and the experimental group 2 was about 4.96 cm longer. As a result of the first survey, the number of leaves and the length of shoots were longer in the experimental group 1 and the experimental group 2 than in the control group.

Next, Table 6 below shows the growth state of lettuce after 20 days from the date of the formaldehyde. In the experiment group 1 and the experiment group 2, a total of 4 times of the soil conditioner was sprayed for 20 days.

Second survey (20 days after formal, 10 days after first survey) Control group Experiment 1 Experiment 2 Length (cm) 16.25 19.08 21.3 Number of leaves (sheets) 12.2 15.8 17.0

The number of leaves was about 3 in experimental group 1 and about 5 in experimental group 2 as compared with the control group. In the plant height, the control group was longer by 4.14 cm, while the experimental group 1 was longer by 6.2 cm and the experimental group 2 was longer by 7.86 cm. As a result of the second investigation, the number of leaves was at least 3 or more in the experimental group compared with the control group, and the experimental group was significantly longer than that of the control group in the plant height.

From Table 5 and Table 6, it can be seen that the growth of lettuce grown in the soil applied with the soil conditioner prepared according to Example 1 and Example 2 was better developed. Thus, it was confirmed that the soil amendment agent prepared according to Example 1 and Example 2 of the present invention promotes plant growth by improving the soil.

In addition, the growth efficiency of the experimental group 2 using the soil amendment agent prepared according to Example 2 including the photosynthetic bacteria was higher than that of the experimental group 1 using the soil amendment agent prepared according to Example 1 which did not contain photosynthetic bacteria It is considered that the addition of photosynthetic bacteria increases the efficacy of the soil amendment agent.

Therefore, the soil amendment agent is prepared at a mixing ratio of 0.7 to 0.9 part by weight of the complex microorganism strain, 0.1 to 0.3 part by weight of lysine, 0.1 to 0.3 part by weight of propolis and 4.5 to 6.5 parts by weight of molasses, relative to 100 parts by weight of water according to the present invention A soil amendment agent having a greatly increased number of microorganisms within the same time period can be produced.

When all the results of the examples, comparative examples and test examples of the present invention are taken into consideration, when lysine, propolis, and molasses are simultaneously used as the culture food of the complex microorganism strain, it is effective to increase the population of the complex microorganism strain. It is estimated that the effect of increasing the population is higher when the proposed mixing ratio is followed.

The present invention has been described with reference to preferred embodiments, comparative examples and test examples. Although the present invention has been described in detail by way of examples, comparative examples and test examples, it is not intended to limit the present invention but merely to illustrate the present invention. Therefore, it will be apparent to those skilled in the art that the scope of the present invention is not limited by these embodiments. The scope of the present invention is defined by the appended claims rather than by the foregoing description, and all differences within the scope of equivalents thereof should be construed as being included in the present invention.

10: Integer steps
20: input step
30: Culture step
40: Measuring step
50: filtration step

Claims

A method for producing a soil conditioner using a combined microorganism strain,
Propolis, molasses, and water (together with seeds of a complex of microorganisms, including Bacillus subtilis , Lactobacillus plantarum , and Saccharomyces cerevisiae) Into a culture vessel;
Culturing the complex microorganism seed introduced into the culture vessel using a culture solution formed by mixing lysine, propolis, molasses and water introduced into the culture vessel;
A measuring step of measuring a pH value of the liquid material cultured in the culturing step; And
And filtering the liquid material whose pH value is measured within a predetermined range in the measuring step to complete the soil remediation agent. The method for producing a soil conditioner according to claim 1,

The method according to claim 1,
The complex microorganism strain is selected from the group consisting of Lactobacillus casei , Lactobacillus acidophilus , Lactobacillus leuconostoc , Lactobacillus brevis , Streptococcus faecalis, characterized in that it further includes at least one of faecalis), Bacillus Pew tree blood carcass (Bacillus putrificus), Bacillus cereus (Bacillus cereus), Pseudomonas fluoro lesson's (Pseudomonas fluorescens), and Oh Spanish way Bruce duck claim (Aspergillus oryzae) Wherein the method comprises the steps of:

The method according to claim 1,
Wherein the complex microbial strain further comprises at least one of green sulfur bacteria, red sulfur bacteria, and red non-sulfur bacteria.

The method according to claim 1,
Wherein the step of introducing is carried out by adding 0.7 to 0.9 parts by weight of a complex microorganism strain, 0.1 to 0.3 parts by weight of lysine, 0.1 to 0.3 parts by weight of propolis and 4.5 to 6.5 parts by weight of molasses to 100 parts by weight of water,
The culturing step is carried out by using a culture medium in which 0.1 to 0.3 parts by weight of lysine, 0.1 to 0.3 parts by weight of propolis and 4.5 to 6.5 parts by weight of molasses are mixed with 100 parts by weight of water, Wherein the method comprises culturing a complex microorganism strain having a part of a microorganism having a specific activity.

5. The method of claim 4,
The culture step is carried out by aeration while simultaneously intermittently stirring 0.7 to 0.9 part by weight of the complex microbial species, 0.1 to 0.3 part by weight of lysine, 0.1 to 0.3 part by weight of propolis and 4.5 to 6.5 parts by weight of molasses, The method comprising culturing a complex microorganism strain of the present invention.

6. The method of claim 5,
Wherein the culturing step is performed by repeating the steps of stirring and aeration for a second time longer than the first time after performing the stirring and the aeration for a first time period so as to perform the intermittent stirring and aeration (EN) METHOD FOR PREPARING SOIL IMPROVER.

6. The method of claim 5,
Wherein the culturing step is carried out with intermittent stirring and aerating while maintaining the temperature of the culture liquid within a range of 35 to 45 ° C.

The method according to claim 1,
Wherein the filtering step comprises filtering the liquid material having a pH value within a range of 3 to 4.5 in the measuring step to complete the soil improving agent.

The method according to claim 1,
The filtering step may include filtering the liquid material whose pH value is measured within a predetermined range in the measuring step at least once using a filter having a pore size of 200 mesh so that the liquid material corresponding to the soil improving agent is discharged from the filter Wherein the method comprises the steps of:

A soil conditioner produced by the method of claim 1.