CN104666360A - Compound preparation for treating secretly-dying disease of penaeus vannamei boone and preparation method of compound preparation - Google Patents
Compound preparation for treating secretly-dying disease of penaeus vannamei boone and preparation method of compound preparation Download PDFInfo
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- CN104666360A CN104666360A CN201310628853.5A CN201310628853A CN104666360A CN 104666360 A CN104666360 A CN 104666360A CN 201310628853 A CN201310628853 A CN 201310628853A CN 104666360 A CN104666360 A CN 104666360A
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Abstract
The invention discloses a compound preparation for treating secretly-dying disease of penaeus vannamei boone and a preparation method of the compound preparation. The compound preparation comprises the following substances in parts by weight: 5-7 parts of bdellovibrio bacteriovorus raw powder, 2-4 parts of compound bacillus raw powder and 1 part of DL-methionine raw powder, wherein the concentration of the bdellovibrio bacteriovorus raw powder is more than 300 million per gram, and the concentration of the compound bacillus raw powder is more than 300 million per gram. The compound preparation has the effect of treating the secretly-dying disease of penaeus vannamei boone, is safe and high in cost performance, has no toxic or side effects and is different from chemicals that cause the drug resistance of pathogenic bacteria and the drug residue; the problems that by virtue of existing drugs, the diseases can only be temporarily relieved, but the problem cannot be basically solved, and the use cost is high can be overcome.
Description
Technical field
The present invention relates to prawn disease medicine, particularly relate to a kind of Penaeus vannamei for the treatment of and steal dead sick compound formulation and preparation method thereof.
Background technology
As everyone knows, China is the first in the world great Chan shrimp state and maximum prawn export state.But Penaeus vannamei steals dead disease very seriously in recent years, has become the number one killer affecting Penaeus vannamei survival rate, make shrimp culture industry meet with development crisis, shrimp culture industry suffers huge strike and loss.Penaeus vannamei steals dead sick cardinal symptom: ill white leg Shrimp must in aubergine, and shrimp body is micro-red, and gastrointestinal is empty, liver enlargement.At present, Penaeus vannamei is stolen and dead sick main is adopted nitrobacteria preparation of splashing, sodium humate adds oxygen supplement powder and treats, but these medicines can only the respite state of an illness once, and can not tackle the problem at its root, use cost is also large.In addition, adopt this kind of chemicals that pathogen can be caused to produce drug resistance and drug residue, bring new problem.
Summary of the invention
For this reason, technical problem to be solved by this invention is to provide a kind of Penaeus vannamei for the treatment of and steals dead sick compound formulation, overcomes the deficiency that prior art exists.
For solving the problems of the technologies described above, the present invention adopts following technical scheme:
Treat Penaeus vannamei and steal a dead sick compound formulation, it is characterized in that forming by the material of following mass ratio: bacteriophagic Bdellovibrio former powder 5-7 part, composite bacillus former powder 2-4 part, 1 part, the former powder of DL-methionine; The concentration (i.e. bacterial content) of the former powder of described bacteriophagic Bdellovibrio is for being greater than 300,000,000/gram, and the concentration of the former powder of described composite bacillus is greater than 300,000,000/gram.
In a preferred embodiment of the invention, 6 parts, the former powder of described bacteriophagic Bdellovibrio, 3 parts, the former powder of described composite bacillus.
The former powder of bacteriophagic Bdellovibrio used in of the present invention and method of counting thereof, be on the books in the patent documentation of ZL201110347334.2 in the patent No., the former powder of composite bacillus is on the books in the patent documentation of ZL201110347608.8 in the patent No..
In addition, the present invention also provides the preparation method of above-mentioned compound formulation, and this preparation method is:
The former powder of bacteriophagic Bdellovibrio, the former powder of composite bacillus and the former powder of DL-methionine is taken by above-mentioned mass ratio; First by former for DL-methionine powder and the former powder uniform stirring mixing of composite bacillus; And then mix with the former powder uniform stirring of bacteriophagic Bdellovibrio; Be uniformly mixed process twice and be all in air-tight state, temperature 25-35 DEG C, mixing speed 200-250r/min, mixing time 1-1.5h.
Adopt technique scheme, the experiment proved that, compound formulation of the present invention has treatment Penaeus vannamei and steals dead sick curative effect, and this replica preparations safe without toxic side effect, cost performance, can not cause pathogen to produce drug resistance and drug residue as chemicals, and existing medicine can be overcome can only the respite state of an illness once, can not tackle the problem at its root, use cost is the problem such as large also.
Accompanying drawing explanation
Below in conjunction with the drawings and specific embodiments, the present invention is described in detail:
Fig. 1 is the therapeutic effect statistical figure of matched group and each test group.
Detailed description of the invention
1. materials and methods
1.1 experiment material
The former powder of bacteriophagic Bdellovibrio, concentration (i.e. bacterial content) is 3.2 hundred million/gram (the high dense bacteriophagic Bdellovibrio former powder method of counting patent No.s: ZL201110347334.2), is purchased from Shanxi biological veterinary feedstuff Engineering Technical Research Centre.
The former powder of high dense composite bacillus, concentration (i.e. bacterial content) is 3.2 hundred million/gram (the high dense composite bacillus former powder method of counting patent No.s: ZL201110347608.8), is purchased from Shanxi biological veterinary feedstuff Engineering Technical Research Centre.
The former powder of DL-methionine, effective content 99%, is purchased from Zhengzhou Hua Tong veterinary drug company limited.
Litopenaeus vannamei compound feed, is purchased from Shanghai Nonghao Feed Co., Ltd..
Suffer from and steal dead sick Penaeus vannamei, totally 8000 tails, by Hangzhou, Cao Shi aquaculture company limited provides.
1.2 treatment Penaeus vannameis steal the preparation of dead sick compound formulation
By the 6:3:1 uniform stirring mixing in mass ratio of former for bacteriophagic Bdellovibrio powder, the former powder of composite bacillus, the former powder of DL-methionine, be treatment Penaeus vannamei and steal dead sick compound formulation.Concrete operations are: existing by 10 kilograms of former powder of DL-methionine, 30 kilograms of former powder uniform stirring mixing of composite bacillus, and then with the 60 kilograms high former powder of dense bacteriophagic Bdellovibrio again uniform stirring mix, the process of being uniformly mixed is in air-tight state, be uniformly mixed process twice and be all in air-tight state, temperature 25-35 DEG C, mixing speed 200-250r/min, mixing time 1-1.5h.
1.3 trouble steal choosing of dead sick Penaeus vannamei
Select just to suffer from and steal dead sick Penaeus vannamei (shrimp must in aubergine, and shrimp body is micro-red, and gastrointestinal is empty, liver enlargement), cultivate in capacity be in the culturing pool of 0.3 cubic metre, each culturing pool 1000 tail.Wherein, culturing pool takes from natural occurrence pond.
1.3 treatment Penaeus vannameis steal the effect of dead sick compound formulation
Setup Experiments 1 matched group and 3 test group, often organize respectively containing 2 Penaeus vannamei morbidity pools (in pond, prawn steals dead disease also in generation, also has the death of prawn).Matched group does not throw in any medicine, and test group uses treatment Penaeus vannamei to steal dead sick compound formulation, with final concentration (dosage in the final pool water within of compound formulation) for 0.1ppm, 0.2ppm, 0.3ppm dosage full pool spilling head, 5 days 1 time, is used in conjunction 4 times.Record the death toll of each group of pond Prawn every day, weighted mean value.Duration of test does not change water.
2 experimental results
Experimental result is shown in Fig. 1.Experimental result shows, with final concentration be 0.1ppm, 0.2ppm, 0.3ppm dosage full pool spilling head treatment Penaeus vannamei steal dead sick compound formulation to Penaeus vannamei steal dead disease there is good therapeutic effect.The operational version that experimental group steals dead sick compound formulation according to treatment Penaeus vannamei is treated latter 6 days, be that Penaeus vannamei that 0.1ppm, 0.2ppm, 0.3ppm dosage full pool spilling head treatment Penaeus vannamei steals dead sick compound formulation is stolen dead disease and all obtained and control completely with final concentration, sick shrimp is all fully recovered, and the prolongation in time of the dead quantity of matched group Penaeus vannamei significantly increases, 4.06 times are added, aggravation time more initial.
3 conclusions
The present invention has prepared a kind of Penaeus vannamei for the treatment of and has stolen dead sick compound formulation, and this compound formulation safe without toxic side effect, cost performance is high, and this compound formulation full pool spilling head under the dosage of 0.1 ~ 0.3ppm has therapeutic effect significantly.The Penaeus vannamei that treatment Penaeus vannamei prepared by the present invention steals dead sick compound formulation and existing report steal dead sick medicine (control just in vain of Penaeus vannamei jejunum. science is breeded fish, 2013, 2:62-62. Penaeus vannamei draws white just treatment one example. contemporary Aquatic product, 2011, 10:38-38.) compare, pathogen can not be caused as chemicals to produce drug resistance and drug residue, and existing medicine can be overcome can only the respite state of an illness once, can not tackle the problem at its root, use cost also the problem such as large (Li Qian. the control that Penaeus vannamei " is stolen dead sick ". science is breeded fish, 2011, 2:53-53.), can fundamentally control to steal dead disease, effect is more lastingly stable, cost is lower, be more suitable for promoting in China.
Claims (4)
1. treat Penaeus vannamei and steal a dead sick compound formulation, it is characterized in that: form by the material of following mass ratio: bacteriophagic Bdellovibrio former powder 5-7 part, composite bacillus former powder 2-4 part, 1 part, the former powder of DL-methionine; The concentration of the former powder of described bacteriophagic Bdellovibrio is for being greater than 300,000,000/gram, and the concentration of the former powder of described composite bacillus is greater than 300,000,000/gram.
2. treatment Penaeus vannamei according to claim 1 steals dead sick compound formulation, it is characterized in that: 6 parts, the former powder of described bacteriophagic Bdellovibrio, 3 parts, the former powder of described composite bacillus.
3. treat the preparation method that Penaeus vannamei steals dead sick compound formulation, it is characterized in that:
The former powder of bacteriophagic Bdellovibrio, the former powder of composite bacillus and the former powder of DL-methionine is taken by the mass ratio of claim 1; First by former for DL-methionine powder and the former powder uniform stirring mixing of composite bacillus; And then mix with the former powder uniform stirring of bacteriophagic Bdellovibrio; Be uniformly mixed process twice and be all in air-tight state, temperature 25-35 DEG C, mixing speed 200-250r/min, mixing time 1-1.5h.
4. treatment Penaeus vannamei according to claim 3 steals the preparation method of dead sick compound formulation, it is characterized in that: 6 parts, the former powder of described bacteriophagic Bdellovibrio, 3 parts, the former powder of described composite bacillus.
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN105941219A (en) * | 2016-05-20 | 2016-09-21 | 广西壮族自治区水产科学研究院 | Prawn Early Mortality Syndrome (EMS) treatment method |
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| CN102352405A (en) * | 2011-11-07 | 2012-02-15 | 山西卫氏鱼康实业有限公司 | Method for counting effective viable bacterium content of Bdellovibrio bacteriovorus powder |
| CN102392070A (en) * | 2011-11-07 | 2012-03-28 | 山西卫氏鱼康实业有限公司 | Quick method for counting content of composite bacillus powder viable bacteria |
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| Publication number | Priority date | Publication date | Assignee | Title |
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| CN105941219A (en) * | 2016-05-20 | 2016-09-21 | 广西壮族自治区水产科学研究院 | Prawn Early Mortality Syndrome (EMS) treatment method |
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Application publication date: 20150603 |