CN102178101B - Prawn feed additive, preparation method thereof and composite feed containing additive - Google Patents

Prawn feed additive, preparation method thereof and composite feed containing additive Download PDF

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CN102178101B
CN102178101B CN201110110216XA CN201110110216A CN102178101B CN 102178101 B CN102178101 B CN 102178101B CN 201110110216X A CN201110110216X A CN 201110110216XA CN 201110110216 A CN201110110216 A CN 201110110216A CN 102178101 B CN102178101 B CN 102178101B
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additive
raw materials
prawn
feed
preparation
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CN102178101A (en
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李会涛
黄河
吕明斌
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Haiyang New Hope Liuhe Feed Co., Ltd.
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Shandong New Hope Liuhe Group Co Ltd
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    • YGENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02ATECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
    • Y02A40/00Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
    • Y02A40/80Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in fisheries management
    • Y02A40/81Aquaculture, e.g. of fish
    • Y02A40/818Alternative feeds for fish, e.g. in aquacultures

Abstract

The invention discloses a prawn feed additive, a preparation method thereof, and a composite feed containing the additive. The additive comprises the following raw materials: astragalus, rheum officinale, Codonopsis pilosula, isatis root, humifuse euphorbia herb and gallnut. The additive may also comprise a synergist. The preparation method comprises: weighing various raw materials according to weight percentage; crushing the raw materials; and feeding the various raw materials into a mixer in turn, and uniformly mixing. The composite feed containing the feed, wherein the weight percentage of the additive is 1 to 3 percent. According to the technical scheme provided by the embodiment of the invention: the additive is made according to a formula of Chinese herb medicine, avoids arousing medicine resistance and producing toxic and side effect, prevents medicine residue in prawns, can obviously increase the growth speed of the prawns and improve the immune activity and disease resistance of the prawns, has high resistance for vibrio anguillarum which is pathogenic bacterium, protects the prawns against diseases, greatly improves survival rate and obviously improves production benefit.

Description

Prawn feed additive and preparation method and the mixed feed that comprises this additive
Technical field
The present invention relates to the prawn culturing technical field, particularly a kind of prawn feed additive and preparation method and the mixed feed that comprises this additive.
Background technology
Prawn (Prawn) belongs to crustacean, is a kind of famous and precious marine products shrimps.Institute's product kind is a lot of in the ocean all over the world, and Chinese prawn, japonicus, Penaeus monodon, banana prawn, penaeus penicillatus, Penaeus Vannmei, green tiger prawn etc. are arranged.Prawn is nutritious because of it, and its meat is soft, is prone to digestion and receives people edible deeply.Because marine fishery resources is depleted, people more and more rely on the demand of prawn and propagate artificially, and therefore, worldwide shrimp culture industry development rapidly.The prawn of culturing in China at present has multiple, mainly is Environment of Litopenaeus vannamei Low, Chinese prawn and japonicus, and nearly 1,300,000 tons of the annual production of China prawn consumes about 1,500,000 tons of feed.
The disease problem of prawn is to hinder the 'bottleneck' restrictions of shrimp culture industry sustainable development all the time; Particularly along with the fast development of prawn culturing and the raising of intensive culture degree; And adopt man-made feeds to feed; The phenomenon of extensive fulminant epidemic disease and large quantities of death often appears in breeding process, and adopt traditional with antibiotic and chemicals be main means pest control method oneself can't satisfy the demands.Its reason is; A large amount of on the one hand use antibiotic and chemicals, and a large amount of chemicals and antibiotic can be distributed to each histoorgan with blood circulation behind the prawn body that is ingested; The immunocompetence of prawn will be weakened gradually; And also cause pathogen to develop immunity to drugs easily, prevention effect descends, and the control cost significantly improves; On the other hand,, directly influence the consumer group's healthy and Environmental security, China's prawn product water outlet competitiveness is restricted owing to the existence of medicament residue.
Summary of the invention
In order to improve the premunition of cultured prawn, make the interior drug residue free of prawn body of breed simultaneously, the embodiment of the invention provides a kind of prawn feed additive and preparation method and the mixed feed that comprises this additive.
In order to realize the foregoing invention purpose, the invention provides a kind of prawn feed additive, wherein; The various raw materials and the percentage by weight of said additive are: the various raw materials and the percentage by weight of said additive are: the Radix Astragali 20%-25%; Rheum officinale 20%-25%, Radix Codonopsis 10%-15%, Radix Isatidis 10%-15%; Wolf's milk 10%-15%, Chinese gall 10%-15%; The percentage by weight sum of above-mentioned raw materials is 100%.
In order to realize the foregoing invention purpose, the present invention also provides a kind of prawn feed additive, wherein; The various raw materials and the percentage by weight of said additive are: the Radix Astragali 20%-25%, rheum officinale 20%-25%, Radix Codonopsis 10%-15%; Radix Isatidis 10%-15%; Wolf's milk 10%-15%, Chinese gall 10%-15%, synergist 5%-10%; The percentage by weight sum of above-mentioned raw materials is 100%.
Said synergist is compound sodium nitrophenolate, sodium humate or algal polysaccharides.
In order to realize the foregoing invention purpose, the present invention provides the preparation method of above-mentioned two kinds of prawn feed additives again, and wherein, said preparation method is:
A, take by weighing various raw materials according to percentage by weight;
B, various raw materials are pulverized;
C, the various raw materials after will pulverizing drop in the mixer successively, mix.
Among the said step b, grinding particle size is 100 orders.
In order to realize the foregoing invention purpose, the present invention also provides the mixed feed that comprises one of above-mentioned two kinds of prawn feed additives, and wherein, the percentage by weight of said additive is 1%-3%.
The percentage by weight of said additive is preferably 1%.
The beneficial effect that the technical scheme that the embodiment of the invention provides is brought is: additive adopts Chinese herbal medicine formula, does not produce drug resistance and toxic and side effect, can the contaminant water environment yet, and drug residue free in the prawn body; Safety and sanitation, environmental protection can significantly improve the speed of growth of prawn; Improve the prawn immunocompetence simultaneously, strengthen the premunition of prawn, good resistance is arranged for main pathogenic bacteria Vibrio anguillarum; Prawn is susceptible disease not, and survival rate is greatly enhanced, and significantly promotes productivity effect.
Description of drawings
Fig. 1 attacks the cumulative mortality of poison back 7d internal reference group and each test group for the said injection of embodiment of the invention Vibrio anguillarum.
The specific embodiment
Embodiment 1 (additive):
A kind of prawn feed additive, wherein, the various raw materials and the percentage by weight of additive are: the Radix Astragali 25%, rheum officinale 25%, Radix Codonopsis 15%, Radix Isatidis 15%, wolf's milk 10%, Chinese gall 10%.
The preparation method of above-mentioned additive is:
A, take by weighing various raw materials according to percentage by weight;
B, various raw materials are pulverized; Grinding particle size is 100 orders, can 100% after promptly pulverizing through 100 mesh sieves.
C, the various raw materials after will pulverizing drop in the mixer successively, mix.
Embodiment 2 (additive):
A kind of prawn feed additive, wherein, the various raw materials and the percentage by weight of additive are: the Radix Astragali 22%, rheum officinale 22%, Radix Codonopsis 12%, Radix Isatidis 12%, wolf's milk 12%, Chinese gall 12% and 8% synergist.
Synergist is an algal polysaccharides.
The preparation method of above-mentioned additive is:
A, take by weighing various raw materials according to percentage by weight;
B, various raw materials are pulverized; Grinding particle size is 100 orders, can 100% after promptly pulverizing through 100 mesh sieves.
C, the various raw materials after will pulverizing drop in the mixer successively, mix.
Embodiment 3 (additive):
A kind of prawn feed additive, wherein, the various raw materials and the percentage by weight of additive are: the Radix Astragali 25%, rheum officinale 20%, Radix Codonopsis 15%, Radix Isatidis 15%, wolf's milk 10%, Chinese gall 10% and 5% synergist.
Synergist is an algal polysaccharides.
The preparation method of above-mentioned additive is:
A, take by weighing various raw materials according to percentage by weight;
B, various raw materials are pulverized; Grinding particle size is 100 orders, can 100% after promptly pulverizing through 100 mesh sieves.
C, the various raw materials after will pulverizing drop in the mixer successively, mix.
Embodiment 4 (additive):
A kind of prawn feed additive, wherein, the various raw materials and the percentage by weight of additive are: the Radix Astragali 23%, rheum officinale 23%, Radix Codonopsis 12%, Radix Isatidis 12%, wolf's milk 10%, Chinese gall 10% and 10% synergist.
Synergist is an algal polysaccharides.
The preparation method of above-mentioned additive is:
A, take by weighing various raw materials according to percentage by weight;
B, various raw materials are pulverized; Grinding particle size is 100 orders, can 100% after promptly pulverizing through 100 mesh sieves.
C, the various raw materials after will pulverizing drop in the mixer successively, mix.
Embodiment 1 (mixed feed):
Contain any one the mixed feed of prawn feed additive of embodiment (additive) 1-4, wherein, the percentage by weight of above-mentioned additive is 1%, and promptly additive evenly adds in the mixed feed according to 1% ratio.
Embodiment 2 (mixed feed):
Contain any one the mixed feed of prawn feed additive of embodiment (additive) 1-4, wherein, the percentage by weight of above-mentioned additive is 1.4%, and promptly additive evenly adds in the mixed feed according to 1.4% ratio.
Embodiment 3 (mixed feed):
Contain any one the mixed feed of prawn feed additive of embodiment (additive) 1-4, wherein, the percentage by weight of above-mentioned additive is 2.2%, and promptly additive evenly adds in the mixed feed according to 2.2% ratio.
Contrast test:
Choose long (1.15 ± 0.04) cm of being of average body, average weight experimentizes for the prawn of (0.03 ± 0.00) g.
Control group adopts the common shrimp feed formula that does not add Chinese herbal medicine; Test group 1 adopts the Chinese herbal medicine of the prescription that has added embodiment 1 (additive), and the percentage by weight that adds is 1%.Test group 2 adopts the Chinese herbal medicine of the prescription that has added embodiment 2 (additive), and the percentage by weight that adds is 1.4%; Test group 3 adopts the Chinese herbal medicine of the prescription that has added embodiment 3 (additive), and the percentage by weight that adds is 2.2%; Concrete prescription is seen table 1.
The common shrimp feed of table 1. is formed with the prescription of the feed that adds Chinese herb feed additive.
Figure 862212DEST_PATH_IMAGE001
Add in table 2. feed Chinese herb feed additive to the influence of prawn growth performance (mean value ± standard error, n=3)
Figure 477738DEST_PATH_IMAGE002
Visible by table 2, the survival rate and the speed of growth of prawn of adding each test group of Chinese herbal medicine in the feed all is significantly increased, and the survival rate of test group 1, whole opisthosoma is heavy and specific growth rate improves 14.2%, 10.6% and 2.2% respectively; The survival rate of test group 2, whole opisthosoma is heavy and specific growth rate improves 15.1%, 12.3% and 2.5% respectively; The survival rate of test group 3, whole opisthosoma is heavy and specific growth rate improves 15.6%, 13.1% and 3.0% respectively;
Add in table 3. feed Chinese herb feed additive to the influence of prawn immune indexes (mean value ± standard error, n=3)
Figure 690545DEST_PATH_IMAGE003
The height of immune indexes has reflected the height of prawn for the resistance of disease.Can find out from table 3; Each item immune indexes that adds the prawn of Chinese herb feed additive feed has all had and has significantly improved (p < 0.05); Wherein, antalzyme activity, phenol oxidase and the superoxide dismutase activity of prawn are compared with control group and are improved 40%, 50.8% and 45.5% respectively in the test group 1; The antalzyme activity of prawn, phenol oxidase and superoxide dismutase activity are compared with control group and are improved 53.3%, 62.2% and 52.7% respectively in the test group 2; The antalzyme activity of prawn, phenol oxidase and superoxide dismutase activity are compared with control group and are improved 60%, 62.4% and 53.8% respectively in the test group 3.
(Vibrio alginolyticus) attacks poison to test group with prawn with Vibrio anguillarum.Confirm that through trial test the malicious concentration of attacking of Vibrio anguillarum is 106 cfu/ml, ID 50 μ l/ tails, record are attacked the cumulative mortality of each group in the 7d of poison back, see Fig. 1.
The result shows: control group prawn cumulative mortality is 54.0%; And each test group cumulative mortality that adds Chinese herbal medicine in the feed significantly is lower than control group (p < 0.05), and the death rate comparison of test group 1, test group 2 and test group 3 has reduced by 28%, 30% and 31% respectively according to group.
Comprehensive above result of the test, Chinese herbal medicine can significantly improve the speed of growth of prawn, improves the prawn immunocompetence simultaneously; Strengthen the premunition of prawn, for main pathogenic bacteria Vibrio anguillarum good resistance is arranged, prawn is susceptible disease not; Survival rate is greatly enhanced, and significantly promotes productivity effect.
The above is merely preferred embodiment of the present invention, and is in order to restriction the present invention, not all within spirit of the present invention and principle, any modification of being done, is equal to replacement, improvement etc., all should be included within protection scope of the present invention.

Claims (5)

1. a prawn feed additive is characterized in that, the various raw materials and the percentage by weight of said additive are: the Radix Astragali 20%-25%; Rheum officinale 20%-25%, Radix Codonopsis 10%-15%, Radix Isatidis 10%-15%; Wolf's milk 10%-15%, Chinese gall 10%-15%, synergist 5%-10%; The percentage by weight sum of above-mentioned raw materials is 100%; Said synergist is compound sodium nitrophenolate, sodium humate or algal polysaccharides.
2. the preparation method of the described prawn feed additive of claim 1 is characterized in that, said preparation method is:
A, take by weighing various raw materials according to percentage by weight;
B, various raw materials are pulverized;
C, the various raw materials after will pulverizing drop in the mixer successively, mix.
3. the preparation method of prawn feed additive according to claim 2 is characterized in that, among the said step b, grinding particle size is 100 orders.
4. a mixed feed that comprises the described prawn feed additive of claim 1 is characterized in that, the percentage by weight of said additive is 1%-3%.
5. mixed feed according to claim 4 is characterized in that, the percentage by weight of said additive is 1%.
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CN102845610B (en) * 2012-08-27 2013-10-16 广东海大集团股份有限公司 Chinese herbal medicine feed additive for improving immunity of Macrobrachium rosenbergii
CN103432242B (en) * 2013-08-08 2016-05-11 泰州市春达动物药业饲料有限公司 A kind of pharmaceutical composition for the treatment of Macrobrachium rosenbergii lean type disease and preparation method thereof
CN103518996A (en) * 2013-09-03 2014-01-22 孙文秀 Shrimp feed additive
CN103844138B (en) * 2013-12-23 2015-07-08 浙江省海洋开发研究院 Mantis shrimp mixed feed and preparation method thereof
CN105231093A (en) * 2015-10-20 2016-01-13 南宁创才阿里科技有限公司 Additive for improving prawn survival rate
CN105231092A (en) * 2015-10-20 2016-01-13 广西中医药大学 Instant compound Chinese herbal medicine powder additive of prawn culture
CN105250439A (en) * 2015-10-20 2016-01-20 广西中医药大学 Preparation method of instant compound Chinese herbal medicine powder additive for prawn culture
CN106259116A (en) * 2016-08-23 2017-01-04 北海金不换水产贸易有限公司 A kind of colored clam artificial raise seedling method being effectively improved yield
CN106360142A (en) * 2016-09-22 2017-02-01 集美大学 Chinese herbal medicine feed additive capable of controlling diseases and promoting growth for penaeus vannamei and preparation method of additive
CN106417985A (en) * 2016-11-28 2017-02-22 宁夏农林科学院 Compound polysaccharide immunity synergist for poultry and technology and application thereof
CN106721652A (en) * 2016-12-20 2017-05-31 梁远雄 A kind of prawn feed
CN106822384A (en) * 2016-12-31 2017-06-13 新昌县迪斯曼科技有限公司 Improve immune immunopotentiator of Chinese lute shrimp and preparation method thereof
CN107260948A (en) * 2017-06-27 2017-10-20 宁国市仙之居家庭农场 The Chinese herbal feed additive and preparation method of a kind of rice shrimp aquaculture
CN108850704A (en) * 2018-06-26 2018-11-23 广东海因特生物技术集团有限公司 Chinese herbal feed additive and its preparation method and application for Penaeus Vannmei
CN115590113A (en) * 2022-10-14 2023-01-13 宁波大学(Cn) Chrysanthemum indicum feed additive and application thereof in prawn culture

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Address after: Laoshan District of Shandong city of Qingdao province Hongkong 266061 Road No. 362 Nonghaiyuan business building 3 floor

Applicant after: Shandong New Hope Liuhe Group Co., Ltd.

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