CN102511697B - Special composite immune-enhancer for Yellow River carp - Google Patents
Special composite immune-enhancer for Yellow River carp Download PDFInfo
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- CN102511697B CN102511697B CN 201210009520 CN201210009520A CN102511697B CN 102511697 B CN102511697 B CN 102511697B CN 201210009520 CN201210009520 CN 201210009520 CN 201210009520 A CN201210009520 A CN 201210009520A CN 102511697 B CN102511697 B CN 102511697B
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- yellow river
- enhancer
- river carp
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Abstract
The invention relates to a special composite immune-enhancer for Yellow River carp. The special composite immune-enhancer for the Yellow River carp is characterized by being prepared from the following components in percentage by weight: 20-50% of chitosan oligosaccharide, 5-30% of vitamin C polyphosphate, 10-25% of pachymaran, 5-25% of rhubarb polysaccharides, 5-20% of lycium barbarum polysaccharide, 5-15% of astragalus polysaccharide; in addition, the usage addition amount of the special composite immune-enhancer is 0.5-1% of the weight of a feed. The special composite immune-enhancer disclosed by the invention has the advantages that: (1) the special composite immune-enhancer can be directly added to the feed of the Yellow River carp for feeding through oral administration, and can be mixed in the feed to prepare a medical bait material for feeding the Yellow River carp after dissolved in the water; (2) the special composite immune-enhancer does not have toxic and side effects, residue and drug resistance, is environment-friendly and does not have bad influence on palatability of the feed; (3) the special composite immune-enhancer can remarkably improve the immunity and the disease-resisting capability of the Yellow River carp, can be applied to prevention on bacterial diseases and virosis of the Yellow River carp, and can be also applied to the therapy of bacterial disease occurrence period of the Yellow River carp; and (4) a production process is simple.
Description
Technical field
The present invention relates to a kind of the Yellow River carp feed addictive, be specifically related to a kind of special composite immune-enhancer for Yellow River carp.
Background technology
The Yellow River carp is one of China tradition four your name fishes, one of at present important culturing economic fish.Because intensive culture in recent years, the fish disease incidence increases greatly.The use of antibiotic and medicine causes pathogenic bacteria to produce drug resistance, has also increased the danger of medicament residue simultaneously, has had a strong impact on the quality safety of cultured fishes products.For improving the resistance against diseases of cultured fishes, the exploitation of immunostimulant for fish is paid close attention to widely.Yet, there is not yet report for the special immune reinforcing agent of the exploitations such as the behaviouristics characteristic of intensive culture the Yellow River carp, growth characteristic, feeding technology, breeding environment.Therefore, development the Yellow River carp special immune reinforcing agent is to improve the Yellow River carp autoimmunity, strengthen resistance against diseases, reduce various kinds of drug consumption in the breeding process, be of great significance to efficient, healthy, sustainability future development tool promoting the Yellow River carp aquaculture.
Summary of the invention
Purpose of the present invention can strengthen the special composite immune-enhancer for Yellow River carp of cultivation the Yellow River carp autoimmunity just for a kind of suitable the Yellow River carp intensive culture characteristics are provided.
Purpose of the present invention can realize by following technique measures:
Special composite immune-enhancer for Yellow River carp of the present invention is mixed by chitosan oligosaccharide 20-50%, LAPP 5-30%, pachymaran 10-25%, rhubarb polyoses 5-25%, LBP-X 5-20% and the astragalus polyose 5-15% of percentage by weight.
Above-mentioned constituent after pulverizing mixed by weight percentage get final product.During use, its addition is 0.5 ~ 1% of feeding quality.
The extraction of the pachymaran described in the present invention adopts following known technology to be prepared from:
Take by weighing Poria cocos 100g, put into stainless-steel pan after the pulverizing, add entry 1L, shake up, boil 1h, take out cooling, use filtered through gauze, collect filtrate, through as above operation, merging filtrate through the centrifugal 10min of 2500 r/min centrifuges, is got supernatant to filter residue again, filtrate is concentrated, adds concentration again and be 98% ethanol, regulates concentration to 80%, stir, leave standstill, with vacuum filtration pump suction filtration, precipitation is washed 2 times with ethanol or acetone, is placed on drying in the drying box, namely gets the polysaccharides of traditional Chinese medicine crude product; The gained polysaccharide is pulverized rear 100 mesh sieves of crossing namely get used pachymaran.
The extracting method of the rhubarb polyoses described in the present invention, LBP-X and the astragalus polyose all extracting method with pachymaran is identical.
Immunopotentiator described in the present invention has the following advantages:
(1) can directly add in the carp feed of the Yellow River, oral throwing something and feeding also can be admixed the dissolving of additive water and be made bait feeding in the feed.
(2) have no side effect, noresidue, without the resistance to the action of a drug, environmental protection, the feed palatability is had no adverse effects.
(3) can conspicuousness improve the Yellow River carp immunity and resistance against diseases, both can be used for the prevention of the Yellow River carp bacteriosis and viral disease, also can be used for the Yellow River treatment of carp bacteriosis emergence period;
(4) production technology is simple.
The specific embodiment
The present invention is further described below with reference to embodiment:
Embodiment 1
Take by weighing respectively chitosan oligosaccharide 30kg, LAPP 20kg, pachymaran 20kg, rhubarb polyoses 10kg, LBP-X 10kg, astragalus polyose 10kg, mix and get final product.
Embodiment 2
Take by weighing respectively chitosan oligosaccharide 20kg, LAPP 10kg, pachymaran 20kg, rhubarb polyoses 20kg, LBP-X 15kg, astragalus polyose 15kg, mix and get final product.
Embodiment 3
Take by weighing respectively chitosan oligosaccharide 50kg, LAPP 5kg, pachymaran 15kg, rhubarb polyoses 15kg, LBP-X 5kg, astragalus polyose 10kg, mix and get final product.
Adopt composite immune reinforcing agent of the present invention as follows as the concrete experimental result of the feed addictive of the Yellow River carp:
The addition that uses of composite immune reinforcing agent of the present invention is 1% of feed weight.
Experimental example 1
Experiment the Yellow River carp 240 tails, initial body weight is 68 ± 1.5g.The experimental group feed adds composite immune reinforcing agent 0.5% in normal diet, the control group normal diet of throwing something and feeding, every group of 3 repetitions, totally 6 breeding barrels, every barrel of 20 tails.Cultivating system is the flowing water culture system, and breeding barrel is diameter 50cm, the conical plastic bucket of high 60cm.Water temperature is 24 ± 1 ℃ in the breeding process.Culture-cycle is 30 days.Got the detection NO content in plasma on the 30th day, and every tail experiment fish injection 1 * 10
8The Aeromonas hydrophila suspension 0.2mL of CFU/mL records cumulative mortality in 7 days.
The result shows that this composite immune reinforcing agent of throwing something and feeding did not affect the growth of the Yellow River carp in 30 days and ingests, and can significantly raise blood plasma lysozyme activity and NO content obviously reduce Aeromonas hydrophila and attacks the rear the Yellow River of poison carp cumulative mortality.
Experimental example 2
Experiment the Yellow River carp 240 tails, initial body weight is about 145g.Cultivating system is the flowing water culture system, and breeding barrel is diameter 50cm, the conical plastic bucket of high 60cm.Water temperature is 25 ± 1 ℃ in the breeding process.Every tail experiment fish injection 1 * 10 after the experiment fish cultivates 15 days with normal diet
8CFU/mL Aeromonas hydrophila 0.2mL.Then be divided into two groups, the experimental group feed adds composite immune reinforcing agent 1% in normal diet, the control group normal diet of throwing something and feeding, every group of 3 repetitions, totally 6 breeding barrels, every barrel of 20 tails.Culture-cycle is 14 days, gets blood plasma respectively at 7d and 14d, detects NO content, and records the cumulative mortality of each group experiment fish in 14 days.
The result shows that behind infected by Aeromonas hydrophila, this composite immune reinforcing agent can obviously reduce the Yellow River carp cumulative mortality.
Experimental example 3
Experiment the Yellow River carp 150 tails, initial body weight is 50 ± 1g.The experimental group feed adds composite immune reinforcing agent 1% in normal diet, the control group normal diet of throwing something and feeding, every group of 3 repetitions, every cylinder 25 tails.Cultivating system is water purification glass jar cultivating system, and the glass jar specification is 80cm * 60cm * 60cm.Water temperature is 25 ± 1 ℃ in the breeding process.Rear 3 days every cylinders of experiment beginning add 3 * 10
7The kinds of fish Rhabdovirus nutrient solution 1.5mL of TCID50/mL, the per 12 hours observation experiment fish death rates.
The result shows that behind high dose kinds of fish Rhabdovirus virus infections, this composite immune reinforcing agent energy conspicuousness prolongs the life span of the Yellow River carp.
Claims (1)
1. special composite immune-enhancer for Yellow River carp, it is characterized in that: described reinforcing agent is to be mixed by the chitosan oligosaccharide 20-50% of percentage by weight, LAPP 5-30%, pachymaran 10-25%, rhubarb polyoses 5-25%, LBP-X 5-20% and astragalus polyose 5-15%; Use addition to be 0.5 ~ 1% of feed weight.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 201210009520 CN102511697B (en) | 2012-01-13 | 2012-01-13 | Special composite immune-enhancer for Yellow River carp |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 201210009520 CN102511697B (en) | 2012-01-13 | 2012-01-13 | Special composite immune-enhancer for Yellow River carp |
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| Publication Number | Publication Date |
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| CN102511697A CN102511697A (en) | 2012-06-27 |
| CN102511697B true CN102511697B (en) | 2013-01-02 |
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| Application Number | Title | Priority Date | Filing Date |
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| CN 201210009520 Expired - Fee Related CN102511697B (en) | 2012-01-13 | 2012-01-13 | Special composite immune-enhancer for Yellow River carp |
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Families Citing this family (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102669497A (en) * | 2012-06-28 | 2012-09-19 | 迟舜雨 | Feed for improving immunity of carps |
| CN104758308A (en) * | 2014-12-10 | 2015-07-08 | 北京康华远景科技股份有限公司 | Traditional Chinese medicine oral liquid capable of improving immunity of livestock and poultry and preparation method thereof |
| CN104957418A (en) * | 2015-06-23 | 2015-10-07 | 通威股份有限公司 | Cyprinus carpio overwintering compound pellet feed |
| CN109329223A (en) * | 2018-09-10 | 2019-02-15 | 中国农业科学院蚕业研究所 | A kind of pathogenetic method of reduction silkworm blood type purulence |
| CN110477218A (en) * | 2019-08-13 | 2019-11-22 | 河南科技大学 | A kind of nutrition prescription preventing the Yellow River carp hemorrhage and preparation method |
Family Cites Families (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101480222A (en) * | 2008-11-19 | 2009-07-15 | 广东海洋大学 | Complex immunopotentiator for Rachycentron canadum |
| CN101961482A (en) * | 2010-06-23 | 2011-02-02 | 天津生机集团股份有限公司 | Immunity enhancer for aquatic animals |
| CN101991026B (en) * | 2010-11-24 | 2012-02-29 | 广州海因特生物技术有限公司 | Complex immunity reinforcing agent for improving stress resistance of black carp and preparation method thereof |
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Granted publication date: 20130102 Termination date: 20160113 |