CN102294017B - Probiotics oral liquid for animal and preparation method thereof - Google Patents
Probiotics oral liquid for animal and preparation method thereof Download PDFInfo
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- CN102294017B CN102294017B CN201110216569A CN201110216569A CN102294017B CN 102294017 B CN102294017 B CN 102294017B CN 201110216569 A CN201110216569 A CN 201110216569A CN 201110216569 A CN201110216569 A CN 201110216569A CN 102294017 B CN102294017 B CN 102294017B
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- probiotic bacteria
- oral liquid
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- enterococcus faecalis
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Abstract
The invention discloses a probiotics oral liquid for animal and preparation method thereof. The probiotics oral liquid comprises 45-50 volume portions of microzyme, 25-30 volume portions of Enterococcus faecalis and 15-20 volume portions of Aspergillus oryzae, wherein, the concentration of colistine sulfate in the probiotics oral liquid is 40-80 mg/ml, and the concentration of Levofloxacin is 4-8 mg/ml. The preparation method comprises the following steps: mixing 45-50 volume portions of microzyme, 25-30 volume portions of Enterococcus faecalis and 15-20 volume portions of Aspergillus oryzae uniformly to obtain a probiotics liquid, then adding 40-80 mg of colistine sulfate and 4-8 mg of Levofloxacin in per 1 ml of the probiotics oral liquid, after fully dissolving stirring uniformly. The probiotics oral liquid disclosed in the invention can control animal bacterial infections without influencing the growth of animal, and can promote the rapid growth of animal.
Description
Technical field
The present invention relates to a kind of poultry oral liquid and preparation method thereof, the particularly a kind of poultry probiotic bacteria oral liquid that can prevent and treat bacterial epidemic diseases such as yellow scour of piglet, Hakuri, red diarrhea, Bacillus pasteurii disease, streptococcicosis, poultry salmonellosis, colibacillosis, Frustrate blood and mycoplasma and preparation method thereof.
Background technology
China is a large agricultural country, is again that an animal husbandry flourishing big country, particularly domestic animal pig poultry production export every Western Europe country, for the mankind make certain contribution.But the infectious disease of domestic animal pig poultry is to influence the key issue that aquaculture is produced always.The 7-8 month in 2005, the epidemic situation that pig streptococcicosis takes place Ziyang, China Sichuan has caused enormous economic loss to animal husbandry, people's infection morbidity 204 people, dead 38 examples.Primary disease has the morbidity report in more than 20 countries and regions such as the U.S., Holland, Germany, Ireland, Japan.The salmonellosis of common pilosity, enterohemorrhagic ETEC infect, and some countries are popular in West Europe recently, serious threat people's health and life security.Also have viral infectious such as SARS, popular in 32 countries and regions, the whole world in 2002-2003, the accumulative total people infects SARS case 8422 examples, dead 916 examples, and mortality rate accounts for 20%.According to statistics, 1959-2005, Hong Kong is compelled to destroy millions of of live chickens because of high pathogenic avian influenza takes place, and this disease more than 20 countries and regions in the world is popular.In recent years, pig blue-ear disease epidemic situations such as (claiming pig breeding and breathing syndrome again) happens occasionally in China.These eqpidemic diseases not only influence the development of herding production sparetime university, are again the infectious disease of infecting both domestic animals and human simultaneously, the serious harm people's health and lives safety.In order effectively to improve animal immunizing power, the generation of prevention zoonosis is necessary to study a kind of poultry probiotic bacteria oral liquid, to prevent and treat the generation of these bacterial infectious diseases.
Summary of the invention
Technical problem to be solved by this invention is; To occurring various infectious disease in the existing livestock breeding industry easily; And influence the deficiency of animal husbandry development and human health; Provide a kind of and can prevent and treat the fowl bacterial infectious disease, do not influence its growth promoter again and can promote poultry probiotic bacteria oral liquid of growth and preparation method thereof.
For solving the problems of the technologies described above, the present invention provides a kind of poultry probiotic bacteria oral liquid.By volume contain 45～50 parts in yeast in portion this probiotic bacteria oral liquid of meter; 15～20 parts of 25～30 parts of enterococcus faecalis and aspergillus oryzaes; And the concentration of colistine sulfate is 40～80mg/ml in this probiotic bacteria oral liquid, and the concentration of levofloxacin is 4～8mg/ml.
The present invention also provides a kind of method for preparing of poultry probiotic bacteria oral liquid, and it comprises the following steps: 1. yeast to be inoculated in potato glucose soup culture medium, and is placed in 28～34 ℃ the calorstat and cultivates; Enterococcus faecalis is inoculated in nutrient broth medium, and is placed in 34～37 ℃ the calorstat and cultivates; Aspergillus oryzae is inoculated in potato glucose soup culture medium, and is placed in 28～34 ℃ of calorstats and cultivates; 2. after above-mentioned yeast, enterococcus faecalis and aspergillus oryzae are cultivated 18～24 hours respectively, 45～50 parts in the yeast after by volume part will be cultivated, 15～20 parts of mix homogeneously of 25～30 parts of enterococcus faecalis and aspergillus oryzae form probiotic bacteria liquid; 3. by adding 40～80mg colistine sulfate in one milliliter of probiotic bacteria liquid,, fully after the dissolving, mix to stir well and evenly promptly get by adding 4～8mg levofloxacin in one milliliter of probiotic bacteria liquid.
One preferred embodiment of this poultry probiotic bacteria oral liquid is by volume to contain 50 parts in yeast, 20 parts of 30 parts of enterococcus faecalis and aspergillus oryzaes in portion this probiotic bacteria oral liquid of meter.
In the said method, the yeast concentration after the cultivation is 5 * 10
8～5 * 10
9Cfu/ml, the concentration of this enterococcus faecalis is 5 * 10
7～5 * 10
8Cfu/ml, the concentration of this aspergillus oryzae is 1 * 10
7～1 * 10
8Cfu/ml.
The yeast that adopts among the present invention is generally a kind of or its mixture in Candida utilis, candida tropicalis bacterium and the cereuisiae fermentum.
Bacterial epidemic diseases such as yellow scours, Hakuri, red diarrhea, poultry Bacillus pasteurii disease, streptococcicosis, poultry salmonellosis, colibacillosis, fowl Frustrate blood and mycoplasma are common, the frequently-occurring diseases of poultry.With the antibiotic etc treatment, the growth, growth, the severe patient that influence poultry become the stiff fowl of cad pig, cause enormous economic loss to aquaculture as single.
Form very much by the live probiotics of strong adaptability (comprising yeast, enterococcus faecalis and aspergillus oryzae) and medicine growth regulator (comprising colistine sulfate and levofloxacin) by strong animal alimentary canal is had of the natural bacterial drug resistance that filters out for poultry probiotic bacteria oral liquid of the present invention; Thereby poultry probiotic bacteria oral liquid of the present invention can be used for preventing and treating bacterial epidemic diseases such as yellow scours, Hakuri, red diarrhea, poultry Bacillus pasteurii disease, streptococcicosis, poultry salmonellosis, colibacillosis, fowl Frustrate blood and mycoplasma; And through using proof; Poultry probiotic bacteria oral liquid of the present invention not only can be cured various bacterial epidemic diseases rapidly; And do not influence animal growth and development, and can promote fast growth of animal.
The general using dosage of poultry probiotic bacteria oral liquid of the present invention is every fowl poultry 2-4 milliliter, every day 1-2 time.
The invention has the advantages that:
1, the present invention adopts multiple drug resistance live probiotics and medicine growth regulator to form through the scientific formula processing and refining first; Technological process is simple, and production cost is low, through up to a hundred clinical trials checking repeatedly for many years; Curative effect is present optimal high-tech innovative product very significantly.
2, poultry probiotic bacteria oral liquid of the present invention contains medicine growth regulator (comprising colistine sulfate and levofloxacin) efficiently; It can utterly destroy the pathogen of bacterial infectious disease; The bacterial epidemic disease of poultry is cured very soon, but it has killed the intravital part probiotic bacteria of poultry simultaneously, has destroyed fowl and has raiseeed intravital microecological balance; Reduce Abwehrkraft des Koepers, influenced the growth promoter of animal.Owing to contain a large amount of multiple drug resistance live probiotics crowds (comprising yeast, enterococcus faecalis and aspergillus oryzae) in the poultry probiotic bacteria oral liquid of the present invention; It gets into livestock and poultry alimentary tract simultaneously; Make the interior little ecology of environment of animal body obtain balance again very soon; And can greatly improve animal immunizing power, promote fast growth of animal.Like this, the two exists drug resistance live probiotics in the poultry probiotic bacteria oral liquid of the present invention and medicine growth regulator simultaneously, interknits, complement one another, and Synergistic, indispensable, thus the good action that performance prevents and treats eqpidemic disease and promotes to grow.
The specific embodiment
Embodiment 1:
1. Candida utilis is inoculated in potato glucose soup culture medium, and is placed in 28 ℃ the calorstat and cultivates; Enterococcus faecalis is inoculated in nutrient broth medium, and is placed in 34 ℃ the calorstat and cultivates; Aspergillus oryzae is inoculated in potato glucose soup culture medium, and is placed in 28 ℃ of calorstats and cultivates; 2. after above-mentioned Candida utilis, enterococcus faecalis and aspergillus oryzae were cultivated 18 hours respectively, 25 parts of 45 parts in yeast, enterococcus faecalis and 15 parts of mix homogeneously of aspergillus oryzae after by volume part meter will be cultivated formed probiotic bacteria liquid; 3. by adding the 40mg colistine sulfate in one milliliter of probiotic bacteria liquid,, fully after the dissolving, mix and stir well the even poultry probiotic bacteria oral liquid of the present invention that promptly gets by adding the 4mg levofloxacin in one milliliter of probiotic bacteria liquid.
With 30 of the above-mentioned poultry probiotic bacteria oral liquid treatment yellow scour of piglet that is mixed with, 2 milliliters of every this prescriptions of spray feed, every day at the upper and lower noon each once, only take twice, 30 ill piglet and promptly fully recover, cure rate is 100%, and back well-grown more.
Embodiment 2:
1. the candida tropicalis bacterium is inoculated in potato glucose soup culture medium, and is placed in 31 ℃ the calorstat and cultivates; Enterococcus faecalis is inoculated in nutrient broth medium, and is placed in 35 ℃ the calorstat and cultivates; Aspergillus oryzae is inoculated in potato glucose soup culture medium, and is placed in 31 ℃ of calorstats and cultivates; 2. after above-mentioned candida tropicalis bacterium, enterococcus faecalis and aspergillus oryzae were cultivated 21 hours respectively, 28 parts of 47 parts in yeast, enterococcus faecalis and 17 parts of mix homogeneously of aspergillus oryzae after by volume part meter will be cultivated formed probiotic bacteria liquid; 3. by adding the 60mg colistine sulfate in one milliliter of probiotic bacteria liquid,, fully after the dissolving, mix and stir well the even poultry probiotic bacteria oral liquid of the present invention that promptly gets by adding the 6mg levofloxacin in one milliliter of probiotic bacteria liquid.
With 12 of the above-mentioned poultry probiotic bacteria oral liquid treatment piglet diarrhea that is mixed with, take off syringe needle with syringe and irritate the amount of taking 4ml/ head every day, only irritates 12 piglets recovery from illness of clothes, and cure rate is 100%.
Embodiment 3:
1. cereuisiae fermentum is inoculated in potato glucose soup culture medium, and is placed in 34 ℃ the calorstat and cultivates; Enterococcus faecalis is inoculated in nutrient broth medium, and is placed in 37 ℃ the calorstat and cultivates; Aspergillus oryzae is inoculated in potato glucose soup culture medium, and is placed in 34 ℃ of calorstats and cultivates; 2. after above-mentioned cereuisiae fermentum, enterococcus faecalis and aspergillus oryzae were cultivated 24 hours respectively, 30 parts of 50 parts in yeast, enterococcus faecalis and 20 parts of mix homogeneously of aspergillus oryzae after by volume part meter will be cultivated formed probiotic bacteria liquid; 3. by adding the 80mg colistine sulfate in one milliliter of probiotic bacteria liquid,, fully after the dissolving, mix and stir well the even poultry probiotic bacteria oral liquid of the present invention that promptly gets by adding the 8mg levofloxacin in one milliliter of probiotic bacteria liquid.
With 20 of the above-mentioned poultry probiotic bacteria oral liquid treatment piglet red dysenteries that is mixed with, 3 milliliters of every this prescriptions of spray feed, every day at the upper and lower noon each once, only take twice, 20 ill piglet and promptly fully recover, cure rate is 100%, and back well-grown more.
Claims (5)
1. poultry probiotic bacteria oral liquid; It is characterized in that; By volume contain 45～50 parts in yeast in portion this probiotic bacteria oral liquid of meter, 15～20 parts of 25～30 parts of enterococcus faecalis and aspergillus oryzaes, and the concentration of colistine sulfate is 40～80mg/ml in this probiotic bacteria oral liquid; The concentration of levofloxacin is 4～8mg/ml, and this yeast is a kind of or its mixture in Candida utilis, candida tropicalis bacterium and the cereuisiae fermentum; This yeast concentration is 5 * 10
8～5 * 10
9Cfu/ml, the concentration of this enterococcus faecalis is 5 * 10
7～5 * 10
8Cfu/ml, the concentration of this aspergillus oryzae is 1 * 10
7～1 * 10
8Cfu/ml.
2. poultry probiotic bacteria oral liquid according to claim 1 is characterized in that, by volume contains 50 parts in yeast, 20 parts of 30 parts of enterococcus faecalis and aspergillus oryzaes in portion this probiotic bacteria oral liquid of meter.
3. the method for preparing of the said poultry probiotic bacteria of claim 1 oral liquid is characterized in that comprising the following steps: 1. yeast being inoculated in potato glucose soup culture medium, and is placed in 28～34 ℃ the calorstat and cultivates; Enterococcus faecalis is inoculated in nutrient broth medium, and is placed in 34～37 ℃ the calorstat and cultivates; Aspergillus oryzae is inoculated in potato glucose soup culture medium, and is placed in 28～34 ℃ of calorstats and cultivates; 2. after above-mentioned yeast, enterococcus faecalis and aspergillus oryzae are cultivated 18～24 hours respectively, 45～50 parts in the yeast after by volume part meter will be cultivated, 15～20 parts of mix homogeneously of 25～30 parts of enterococcus faecalis and aspergillus oryzae form probiotic bacteria liquid; 3. by adding 40～80mg colistine sulfate in one milliliter of probiotic bacteria liquid, by adding 4～8mg levofloxacin in one milliliter of probiotic bacteria liquid, fully after the dissolving, mix stir well even.
4. the method for preparing of poultry probiotic bacteria oral liquid according to claim 3 is characterized in that, 50 parts in the yeast after above-mentioned steps will be cultivated in 2., 20 parts of mix homogeneously of 30 parts of enterococcus faecalis and aspergillus oryzae.
5. the method for preparing of poultry probiotic bacteria oral liquid according to claim 3 is characterized in that, the yeast concentration after this cultivation is 5 * 10
8～5 * 10
9Cfu/ml, the concentration of this enterococcus faecalis is 5 * 10
7～5 * 10
8Cfu/ml, the concentration of this aspergillus oryzae is 1 * 10
7～1 * 10
8Cfu/ml.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201110216569A CN102294017B (en) | 2011-08-01 | 2011-08-01 | Probiotics oral liquid for animal and preparation method thereof |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201110216569A CN102294017B (en) | 2011-08-01 | 2011-08-01 | Probiotics oral liquid for animal and preparation method thereof |
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| Publication Number | Publication Date |
|---|---|
| CN102294017A CN102294017A (en) | 2011-12-28 |
| CN102294017B true CN102294017B (en) | 2012-07-11 |
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Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101368165A (en) * | 2008-08-12 | 2009-02-18 | 辽宁医学院 | Probiotic composition for livestock and poultry |
| CN101810247A (en) * | 2009-12-30 | 2010-08-25 | 沈阳科丰牧业科技有限公司 | Complex microbial chicken feed additive |
| CN101874812A (en) * | 2010-05-14 | 2010-11-03 | 宁波市特圣生物饲料有限公司 | Composite bacteria preparation and application thereof in weight increment of immature livestock and control of diarrhea |
-
2011
- 2011-08-01 CN CN201110216569A patent/CN102294017B/en not_active Expired - Fee Related
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101368165A (en) * | 2008-08-12 | 2009-02-18 | 辽宁医学院 | Probiotic composition for livestock and poultry |
| CN101810247A (en) * | 2009-12-30 | 2010-08-25 | 沈阳科丰牧业科技有限公司 | Complex microbial chicken feed additive |
| CN101874812A (en) * | 2010-05-14 | 2010-11-03 | 宁波市特圣生物饲料有限公司 | Composite bacteria preparation and application thereof in weight increment of immature livestock and control of diarrhea |
Non-Patent Citations (2)
| Title |
|---|
| 李巧贤 等.畜牧业生产中细菌素取代抗生素的发展趋势.《畜牧与饲料科学》.2010,第31卷(第6-7期),第233-234页. * |
| 李明 等.益生菌素添加剂应尽快应用于畜牧业生产.《畜牧兽医科技信息》.2005,(第12期),第75-76页. * |
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| CN102294017A (en) | 2011-12-28 |
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Granted publication date: 20120711 Termination date: 20200801 |