CN102511697A - Special composite immune-enhancer for Yellow River carp - Google Patents
Special composite immune-enhancer for Yellow River carp Download PDFInfo
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- CN102511697A CN102511697A CN201210009520XA CN201210009520A CN102511697A CN 102511697 A CN102511697 A CN 102511697A CN 201210009520X A CN201210009520X A CN 201210009520XA CN 201210009520 A CN201210009520 A CN 201210009520A CN 102511697 A CN102511697 A CN 102511697A
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Abstract
The invention relates to a special composite immune-enhancer for Yellow River carp. The special composite immune-enhancer for the Yellow River carp is characterized by being prepared from the following components in percentage by weight: 20-50% of chitosan oligosaccharide, 5-30% of vitamin C polyphosphate, 10-25% of pachymaran, 5-25% of rhubarb polysaccharides, 5-20% of lycium barbarum polysaccharide, 5-15% of astragalus polysaccharide; in addition, the usage addition amount of the special composite immune-enhancer is 0.5-1% of the weight of a feed. The special composite immune-enhancer disclosed by the invention has the advantages that: (1) the special composite immune-enhancer can be directly added to the feed of the Yellow River carp for feeding through oral administration, and can be mixed in the feed to prepare a medical bait material for feeding the Yellow River carp after dissolved in the water; (2) the special composite immune-enhancer does not have toxic and side effects, residue and drug resistance, is environment-friendly and does not have bad influence on palatability of the feed; (3) the special composite immune-enhancer can remarkably improve the immunity and the disease-resisting capability of the Yellow River carp, can be applied to prevention on bacterial diseases and virosis of the Yellow River carp, and can be also applied to the therapy of bacterial disease occurrence period of the Yellow River carp; and (4) a production process is simple.
Description
Technical field
The present invention relates to a kind of the Yellow River carp feed addictive, be specifically related to a kind of the Yellow River carp specific complex immunopotentiator.
Background technology
The Yellow River carp is one of China tradition four your name fishes, one of at present important culturing economic fish.Because intensive culture in recent years, the fish disease incidence increases greatly.The use of antibiotic and medicine causes pathogenic bacteria to produce drug resistance, has also increased the danger of medicament residue simultaneously, has had a strong impact on the quality safety of cultured fishes products.For improving the resistance against diseases of cultured fishes, the exploitation of immunostimulant for fish receives widely and paying close attention to.Yet, do not appear in the newspapers as yet to the special immune reinforcing agent of exploitations such as the behaviouristics characteristic of intensive culture the Yellow River carp, growth characteristic, feeding technology, breeding environment.Therefore; Development the Yellow River carp special immune reinforcing agent is to improve the Yellow River carp autoimmunity; Strengthen resistance against diseases, reduce various kinds of drug consumption in the breeding process, have crucial meaning promoting the Yellow River carp aquaculture to develop to efficient, healthy, sustainability direction.
Summary of the invention
The object of the invention can strengthen the Yellow River carp specific complex immunopotentiator of culturing the Yellow River carp autoimmunity just for a kind of suitable the Yellow River carp intensive culture characteristics are provided.
The object of the invention can be realized through following technique measures:
The Yellow River of the present invention carp specific complex immunopotentiator is mixed by chitosan oligosaccharide 20-50%, vitamin C condensed phosphate 5-30%, pachymaran 10-25%, rhubarb polyoses 5-25%, LBP-X 5-20% and the astragalus polyose 5-15% of percentage by weight.
Above-mentioned constituent after pulverizing mixed by weight percentage get final product.During use, its addition is 0.5 ~ 1% of a feeding quality.
The extraction of the pachymaran described in the present invention adopts following known technology to be prepared from:
Take by weighing Poria cocos 100g, put into stainless-steel pan after the pulverizing, add entry 1L, shake up, boil 1h, take out cooling; Use filtered through gauze, collect filtrating, filter residue is again through as above operation, and merging filtrate is through the centrifugal 10min of 2500 r/min centrifuges; Get supernatant, filtrating concentrates, and adds concentration again and be 98% ethanol, regulates concentration to 80%, stirs; Leave standstill, with vacuum filtration pump suction filtration, deposition is with ethanol or washing with acetone 2 times, is placed in the drying box dryly, promptly gets the Chinese herbal medicine polysaccharide crude; 100 mesh sieves are crossed in gained polysaccharide pulverizing back promptly get used pachymaran.
Rhubarb polyoses described in the present invention, LBP-X and extraction of Astragalus Polysaccharides in Astragalus the method all method for distilling with pachymaran are identical.
Immunopotentiator described in the present invention has the following advantages:
(1) can directly add in the carp feed of the Yellow River, oral throwing something and feeding also can be admixed the dissolving of additive water and made bait feeding in the feed.
(2) have no side effect, noresidue, the no resistance to the action of a drug, environmental protection, the feed palatability is had no adverse effects.
(3) can conspicuousness improve the Yellow River carp immunity and resistance against diseases, both can be used for the prevention of the Yellow River carp bacteriosis and viral disease, also can be used for the Yellow River treatment of carp bacteriosis emergence period;
(4) production technology is simple.
The specific embodiment
To combine embodiment to further describe below the present invention:
Embodiment 1
Take by weighing chitosan oligosaccharide 30kg, vitamin C condensed phosphate 20kg, pachymaran 20kg, rhubarb polyoses 10kg, LBP-X 10kg, astragalus polyose 10kg respectively, mix and get final product.
Embodiment 2
Take by weighing chitosan oligosaccharide 20kg, vitamin C condensed phosphate 10kg, pachymaran 20kg, rhubarb polyoses 20kg, LBP-X 15kg, astragalus polyose 15kg respectively, mix and get final product.
Embodiment 3
Take by weighing chitosan oligosaccharide 50kg, vitamin C condensed phosphate 5kg, pachymaran 15kg, rhubarb polyoses 15kg, LBP-X 5kg, astragalus polyose 10kg respectively, mix and get final product.
Adopt composite immune reinforcing agent of the present invention following as the concrete experimental result of the feed addictive of the Yellow River carp:
The addition that uses of composite immune reinforcing agent of the present invention is 1% of feed weight.
Experimental example 1
Experiment the Yellow River carp 240 tails, initial body weight is 68 ± 1.5g.The experimental group feed adds composite immune reinforcing agent 0.5% in normal diet, the control group normal diet of throwing something and feeding, and every group of 3 repetitions are cultured bucket, every barrel 20 tail for totally 6.Cultivating system is the flowing water cultivating system, and culturing bucket is diameter 50cm, the conical plastic bucket of high 60cm.Water temperature is 24 ± 1 ℃ in the breeding process.Culture-cycle is 30 days.Got the detection NO content in plasma on the 30th day, and every tail experiment fish injection 1 * 10
8The Aeromonas hydrophila suspension 0.2mL of CFU/mL writes down cumulative mortality in 7 days.
The result shows that this composite immune reinforcing agent of throwing something and feeding did not influence the growth of the Yellow River carp in 30 days and ingests, and can significantly raise blood plasma lysozyme activity and NO content obviously reduce Aeromonas hydrophila and attacks the Yellow River, poison back carp cumulative mortality.
Experimental example 2
Experiment the Yellow River carp 240 tails, initial body weight is about 145g.Cultivating system is the flowing water cultivating system, and culturing bucket is diameter 50cm, the conical plastic bucket of high 60cm.Water temperature is 25 ± 1 ℃ in the breeding process.The experiment fish is with normal diet breed every tail experiment fish injection 1 * 10 after 15 days
8CFU/mL Aeromonas hydrophila 0.2mL.Be divided into two groups then, the experimental group feed adds composite immune reinforcing agent 1% in normal diet, the control group normal diet of throwing something and feeding, and every group of 3 repetitions are cultured bucket, every barrel 20 tail for totally 6.Culture-cycle is 14 days, gets blood plasma respectively at 7d and 14d, detects NO content, and writes down the cumulative mortality of each group experiment fish in 14 days.
The result shows that after Aeromonas hydrophila infected, this composite immune reinforcing agent can obviously reduce the Yellow River carp cumulative mortality.
Experimental example 3
Experiment the Yellow River carp 150 tails, initial body weight is 50 ± 1g.The experimental group feed adds composite immune reinforcing agent 1% in normal diet, the control group normal diet of throwing something and feeding, every group of 3 repetitions, every cylinder 25 tails.Cultivating system is a water purification glass jar cultivating system, and the glass jar specification is 80cm * 60cm * 60cm.Water temperature is 25 ± 1 ℃ in the breeding process.Back 3 days every cylinders of experiment beginning add 3 * 10
7The kinds of fish Rhabdovirus nutrient solution 1.5mL of TCID50/mL, per 12 h observation experiment fish death rate.
The result shows that behind high dose kinds of fish Rhabdovirus virus infections, this composite immune reinforcing agent ability conspicuousness prolongs the life span of the Yellow River carp.
Claims (1)
1. the Yellow River carp specific complex immunopotentiator, it is characterized in that: said reinforcing agent is to be mixed by the chitosan oligosaccharide 20-50% of percentage by weight, vitamin C condensed phosphate 5-30%, pachymaran 10-25%, rhubarb polyoses 5-25%, LBP-X 5-20% and astragalus polyose 5-15%; Use addition to be 0.5 ~ 1% of feed weight.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 201210009520 CN102511697B (en) | 2012-01-13 | 2012-01-13 | Special composite immune-enhancer for Yellow River carp |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 201210009520 CN102511697B (en) | 2012-01-13 | 2012-01-13 | Special composite immune-enhancer for Yellow River carp |
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| Publication Number | Publication Date |
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| CN102511697A true CN102511697A (en) | 2012-06-27 |
| CN102511697B CN102511697B (en) | 2013-01-02 |
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Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102669497A (en) * | 2012-06-28 | 2012-09-19 | 迟舜雨 | Feed for improving immunity of carps |
| CN104758308A (en) * | 2014-12-10 | 2015-07-08 | 北京康华远景科技股份有限公司 | Traditional Chinese medicine oral liquid capable of improving immunity of livestock and poultry and preparation method thereof |
| CN104957418A (en) * | 2015-06-23 | 2015-10-07 | 通威股份有限公司 | Cyprinus carpio overwintering compound pellet feed |
| CN109329223A (en) * | 2018-09-10 | 2019-02-15 | 中国农业科学院蚕业研究所 | A kind of pathogenetic method of reduction silkworm blood type purulence |
| CN110477218A (en) * | 2019-08-13 | 2019-11-22 | 河南科技大学 | A kind of nutrition prescription preventing the Yellow River carp hemorrhage and preparation method |
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| CN101480222A (en) * | 2008-11-19 | 2009-07-15 | 广东海洋大学 | Complex immunopotentiator for Rachycentron canadum |
| CN101961482A (en) * | 2010-06-23 | 2011-02-02 | 天津生机集团股份有限公司 | Immunity enhancer for aquatic animals |
| CN101991026A (en) * | 2010-11-24 | 2011-03-30 | 广州海因特生物技术有限公司 | Complex immunity reinforcing agent for improving stress resistance of black carp and preparation method thereof |
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- 2012-01-13 CN CN 201210009520 patent/CN102511697B/en not_active Expired - Fee Related
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| CN101480222A (en) * | 2008-11-19 | 2009-07-15 | 广东海洋大学 | Complex immunopotentiator for Rachycentron canadum |
| CN101961482A (en) * | 2010-06-23 | 2011-02-02 | 天津生机集团股份有限公司 | Immunity enhancer for aquatic animals |
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Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102669497A (en) * | 2012-06-28 | 2012-09-19 | 迟舜雨 | Feed for improving immunity of carps |
| CN104758308A (en) * | 2014-12-10 | 2015-07-08 | 北京康华远景科技股份有限公司 | Traditional Chinese medicine oral liquid capable of improving immunity of livestock and poultry and preparation method thereof |
| CN104957418A (en) * | 2015-06-23 | 2015-10-07 | 通威股份有限公司 | Cyprinus carpio overwintering compound pellet feed |
| CN109329223A (en) * | 2018-09-10 | 2019-02-15 | 中国农业科学院蚕业研究所 | A kind of pathogenetic method of reduction silkworm blood type purulence |
| CN110477218A (en) * | 2019-08-13 | 2019-11-22 | 河南科技大学 | A kind of nutrition prescription preventing the Yellow River carp hemorrhage and preparation method |
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| Publication number | Publication date |
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| CN102511697B (en) | 2013-01-02 |
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