CN1500749A - Modifying agent for microorganism breeding water and preparing method thereof - Google Patents

Modifying agent for microorganism breeding water and preparing method thereof Download PDF

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Publication number
CN1500749A
CN1500749A CNA021452490A CN02145249A CN1500749A CN 1500749 A CN1500749 A CN 1500749A CN A021452490 A CNA021452490 A CN A021452490A CN 02145249 A CN02145249 A CN 02145249A CN 1500749 A CN1500749 A CN 1500749A
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China
Prior art keywords
composite
microorganism cultivation
little algae
molasses
algae
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CNA021452490A
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CN1266054C (en
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瀚 江
江瀚
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SHANGHAI CHUANGBO ECOLOGICAL ENGINEERING Co Ltd
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SHANGHAI CHUANGBO ECOLOGICAL ENGINEERING Co Ltd
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    • YGENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02ATECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
    • Y02A40/00Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
    • Y02A40/80Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in fisheries management
    • Y02A40/81Aquaculture, e.g. of fish

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  • Purification Treatments By Anaerobic Or Anaerobic And Aerobic Bacteria Or Animals (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)

Abstract

The microbial aquatic breeding water improver features its composite microbial bacterial preparation comprising composite bacteria preparation and micro algae, and consists of composite bacteria, micro algae, culture medium, molasses, trace element solution, corn powder, and solidifying material. The preparation process features that all the bacteria seeds are screened from water and sludge for aquatic breeding and are cultured in the same culture medium and composite bacteria preparation and micro algae are solidified compositely. The microbial aquatic breeding water improver can decompose residual bait, excrement, animal and plant remains in the water bottom and harmful gas to purify water and raise the survival rate of aquatic animals.

Description

Microorganism cultivation aquifer amendment and making method
Technical field:
The present invention relates to a kind of microorganism cultivation aquifer amendment and making method, can be used for aquaculture, purify water, field such as environmental protection, belong to aquaculture system improving technology field.
Background technology:
In the culture fishery, culture a large amount of remaining bait of bottom, waters accumulation, drain refuse, plant and animal residues and obnoxious flavour, cause easily that water quality is ruined, pathogenic bacteria breeds in a large number and the aquaculture thing is poisoned.
Summary of the invention:
Purpose of the present invention is exactly a kind of microorganism cultivation aquifer amendment and making method that can improve aquaculture system of invention.
For realizing above purpose, technical scheme of the present invention provides a kind of microorganism cultivation aquifer amendment, it is characterized in that, by the compound formulation that composite fungus agent and little algae are formed, it is formulated by following weight percentages:
Composite bacteria stoste 5-30%
Little algae 30-70%
Molasses 5-30%
Trace element solution 0.5-15%
Each raw material optimum weight proportioning of preparation of the present invention is:
Composite bacteria stoste 20%
Little algae 60%
Molasses 18%
Trace element solution 2%
Composite bacteria of the present invention is one of Rhodopseudomonas palustris, nitrobacteria, plant lactobacillus, streptococcus uberis, saccharomyces cerevisiae, actinomyces, streptomyces, bacillus, methane-oxidizing bacteria, ammonia oxidation bacteria or their mixture; Described little algae is one of the lucky egg capsule algae of ripple, Nannochloropsis oceanica or their mixture.
Making method of the present invention is characterized in that, all bacterial classifications of employing and little algae all are to filter out from the water body of aquaculture and bed mud, harmful algae, soil and cultivated animals body surface; Adopt the bacterial classification compound culture method, all bacterial classifications are cultivated in same substratum; Adopt composite fungus agent and little algae composite curing method; It comprises the following steps:
(1) screening and optimizing bacterial classification and little algae from the water body of aquaculture and bed mud, harmful algae, soil and cultivated animals body surface;
(2) be to cultivate in one of molasses, peptone, sodium-chlor, ferric sulfate, sal epsom, growth factor solution or their mixture all bacterial classifications at same medium component, the ph value is adjusted to 6.8-7.2; Culture temperature is that 30-35 ℃, time are 70-75 hour, obtains composite fungus agent;
(3) composite fungus agent is added inserted in the closed tin fermentation behind little algae, molasses, the trace element solution uniform mixing 24-48 hour, temperature is 25-30 ℃, obtains the microorganism cultivation aquifer amendment of composite fungus agent and little algae;
(4) microorganism being supported straight aquifer amendment adds molasses, Semen Maydis powder and uniformly mixes that to put into diameter be that solidify material about 5mm stirs with rotating speed 60 commentaries on classics/min, under illumination condition, cultivated 60 hours, obtain the microorganism cultivation aquifer amendment of composite curing, the ratio of microorganism cultivation aquifer amendment, molasses, ground rice, solidify material is: 30%, 20%, 30%, 20%.
The composite fungus agent that strain screening is cultivated from the water body of aquaculture and bed mud, it is rich in monomeric protein, amino acid, organized enzyme, antioxidant, VITAMIN and other various physiologically active substances, has oxidation, ammonification, nitrated, phosphorus decomposing, sulfuration and nitrogen fixation, objectionable impurities can be decomposed into sulfurous gas, nitrate etc., instrument does not purify water, and can provide nutrition for the breeding of unicellular algae, the photosynthesis of these algae, produce the breathing of cultivated animals again for pool inner water, organic decomposition provides oxygen, thereby form a good ecological circulation, help the enhancing of aquaculture thing immunological competence and the quickening of the speed of growth, and little algae has decomposing organic matter, increase dissolved oxygen, the ability of absorb polluted matter matter, composite fungus agent has harmful algae with the compound microorganism cultivation aquifer amendment that obtains of little algae and directly subtracts effect extremely, and it can make the algae wall of harmful algae break and cause the frond death of directly turning to be yellow.
Advantage of the present invention is:
1. make that polypeptide key in the bacterium protein of harmful bacterium in the water body, virus decomposes, fracture, with loss of function and dead;
2. organic pollutants such as movement of residual bait and hydrocoles in the decomposition aquaculture water, in and nitrite, the toxicity of materials such as manganese, copper, mercury in the degradation water, the useful vegeto-animal growth and breeding that swims in the formation of the harmful water body of control and the promotion feculent water body, thus water conditioning is being fit to the benign state that the aquaculture thing is grown up healthy and sound;
3. increase the food consumption of aquaculture thing, significantly improve surviving rate and efficiency of feed utilization, thereby reduce feeding cost, improve culture benefit;
4. effectively prevent aquatic products material culture disease to take place, strengthen aquaculture thing pipe intestinal digesting absorptive function;
5. can remove peculiar smell and the effect of oxygenation is arranged, fish and shrimp are not had bad stress reaction;
6. effectively control the generation of the bacteriosis of aquaculture things such as the rotted gill disease of light, seawater fish and shrimp, crab, soft-shelled turtle, tortoise, the frog, shellfish and virus disease and spread, crab is trembled disease, gonorrhoea disease, peach draw disease etc. that obvious curative effects is arranged;
7. guarantee aquaculture safety, efficient, have no side effect, noresidue, pollution-free.
Embodiment:
1. the making method of microorganism cultivation aquifer amendment:
(1). screening and optimizing bacterial classification and little algae from the water body of aquaculture and bed mud, harmful algae, soil and cultivated animals body surface take by weighing by following weight proportion:
Rhodopseudomonas palustris 20%, nitrobacteria 5%, plant lactobacillus 10%, streptococcus uberis 10%, saccharomyces cerevisiae 10%, actinomyces 12%, streptomyces 8%, bacillus 15%, methane-oxidizing bacteria 5%, ammonia oxidation bacteria 5%; Take by weighing substratum by following weight proportion:
Molasses 1%, peptone 0.4%, sodium-chlor 0.2%, ferric sulfate 0.05%, sal epsom 0.02%, growth factor solution 1%, ph value 6.8;
(2). all bacterial classifications are cultivated in same substratum, and culture temperature is that 33 ℃, time are 72 hours, obtains composite fungus agent;
Take by weighing little algae by following weight proportion:
The lucky egg capsule algae 60% of ripple, Nannochloropsis oceanica 40% mix;
Take by weighing composite fungus agent and raw material by following weight proportion:
Composite fungus agent 20%, little algae 60%, molasses 18%, trace element solution 2%;
(3). composite fungus agent added inserted in the closed tin fermentation behind little algae, molasses, the trace element solution uniform mixing 36 hours, temperature is 28 ℃, obtains the microorganism cultivation aquifer amendment of composite fungus agent and little algae.
2. the microorganism cultivation aquifer amendment making method of composite curing:
After the microorganism cultivation aquifer amendment added molasses, Semen Maydis powder and uniformly mix, the solidify material of putting into diameter and being about 5mm is that urethane stirs with rotating speed 60 commentaries on classics/min comprising tamanori, zeolite or organic polymer material, under illumination condition, cultivated 60 hours, obtain the microorganism cultivation aquifer amendment of composite curing, the ratio of microorganism cultivation aquifer amendment, molasses, Semen Maydis powder, solidify material is: 30%, 20%, 30%, 20%.
In use, use the microorganism cultivation aquifer amendment of microorganism cultivation aquifer amendment or composite curing according to different situations:
When using the microorganism cultivation aquifer amendment, should mix 100 times of clear water dilutions, culturing or evenly splashing in the pond the last week of pouring water then, every mu of consumption 1kg splashed once in per ten days, can keep water quality optimum stable; As occur raising the nose above water to breathe and during the phenomenon of the general pool, can directly spray the water surface, occur crab tremble disease, mould pinta, and during rotted gill disease, but dosage use, as finding crab, shrimp trouble gastroenteritis, when not taking food,, can improving surviving rate with the diluent water surface of splashing.
When using solidified microorganism cultivation aquifer amendment, the microorganism cultivation aquifer amendment of composite curing can be put into water, can be suspended in the water at initial 10 minutes, sink at the bottom of the pool after being partly dissolved, but progressively enter the organic pollution materials in the microorganism and little algae decomposition and inversion water body in the water body in the time of in water body, suppress the growth of harmful algae, can decompose mud, residual bait after sinking at the bottom of the pool, remove assorted bacterium.

Claims (6)

1. a microorganism cultivation aquifer amendment is characterized in that, the complex micro organism fungicide of being made up of composite fungus agent and little algae; It is formulated by following weight percentages:
Composite bacteria 5-30%
Little algae 30-70%
Molasses 5-30%
Trace element solution 0.5-15%
2. a microorganism cultivation aquifer amendment is characterized in that, wherein the optimum weight proportioning of each raw material is:
Composite bacteria 20%
Little algae 60%
Molasses 18%
Trace element solution 2%
3. microorganism cultivation aquifer amendment according to claim 1, its characteristics are that described composite bacteria is one of Rhodopseudomonas palustris, nitrobacteria, plant lactobacillus, streptococcus uberis, saccharomyces cerevisiae, actinomyces, streptomyces, bacillus, methane-oxidizing bacteria, ammonia oxidation bacteria or their mixture.
4. microorganism cultivation aquifer amendment according to claim 1, its characteristics are that described little algae is one of the lucky egg capsule algae of ripple, Nannochloropsis oceanica or their mixture.
5. the making method of a microorganism cultivation aquifer amendment is characterized in that, all bacterial classifications of employing and little algae all are to filter out from the water body of aquaculture and bed mud, harmful algae, soil and cultivated animals body surface; Adopt the bacterial classification compound culture method, all bacterial classifications are cultivated in same substratum; Adopt composite fungus agent and little algae composite curing method; It comprises the following steps:
(1) screening and optimizing bacterial classification and little algae from the water body of aquaculture and bed mud, harmful algae, soil and cultivated animals body surface;
(2) be to cultivate in one of molasses, peptone, sodium-chlor, ferric sulfate, sal epsom, growth factor solution or their mixture all bacterial classifications at same medium component, the ph value is adjusted to 6.8-7.2; Culture temperature is that 30-35 ℃, time are 70-75 hour, obtains composite fungus agent;
(3) composite fungus agent is added inserted in the closed tin fermentation behind little algae, molasses, the trace element solution uniform mixing 24-48 hour, temperature is 25-30 ℃, obtains the microorganism cultivation aquifer amendment of composite fungus agent and little algae;
(4) the microorganism cultivation aquifer amendment is added to put into diameter after molasses, Semen Maydis powder uniformly mix be that solidify material about 5mm stirs with rotating speed 60 commentaries on classics/min, under illumination condition, cultivated 48-72 hour, obtain the microorganism cultivation aquifer amendment of composite curing, the ratio of microorganism cultivation aquifer amendment, molasses, Semen Maydis powder, solidify material is: 30%, 20%, 30%, 20%.
6. the making method of microorganism cultivation aquifer amendment according to claim 5, its characteristics are that described solidify material is tamanori, zeolite, organic polymer material etc., and the main component of organic polymer material is a urethane.
CNB021452490A 2002-11-12 2002-11-12 Modifying agent for microorganism breeding water and preparing method thereof Expired - Fee Related CN1266054C (en)

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CN1313395C (en) * 2005-03-09 2007-05-02 西北农林科技大学 Ultra concentrated algae bacteria microecological equipment suspension type water quality modifier and its preparation method
CN101898835A (en) * 2010-07-26 2010-12-01 宁波大学 Method for purifying aquatic water by using microbes and plants in combination
CN101376877B (en) * 2007-08-31 2011-07-27 天津中敖生物科技有限公司 Compound microecological preparation for purifying cultivation water, preparation formulation thereof and preparation processes of the preparation
CN102153208A (en) * 2010-12-31 2011-08-17 浙江大学 Aquaculture water purifying agent
CN102206585A (en) * 2011-04-29 2011-10-05 上海水大环境工程有限公司 Composite microbial preparation for purifying water and preparation method thereof
CN102219309A (en) * 2011-03-13 2011-10-19 沧州市中信生物科技有限公司 SWater purifying agent with quick result
CN102524117A (en) * 2011-12-12 2012-07-04 中国水产科学研究院黑龙江水产研究所 Method for improving water quality of fresh water culture pond and promoting fish growth
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CN102674563A (en) * 2012-06-04 2012-09-19 广东金海润生物科技有限公司 Novel environment-friendly efficient microorganism water quality purifying mixture and preparation method thereof
CN102765814A (en) * 2012-07-05 2012-11-07 昆明豪原特自控有限公司 Ecological water treatment method
CN102765813A (en) * 2012-07-05 2012-11-07 昆明豪原特自控有限公司 Method for treating industrial and domestic sewage by active organisms
CN102765776A (en) * 2012-07-05 2012-11-07 昆明豪原特自控有限公司 Active biological water purifying agent and preparation method thereof
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CN106630176A (en) * 2016-09-29 2017-05-10 南宁海世界生物科技有限公司 Water quality modifier for fishery culture and preparation method thereof
CN106719269A (en) * 2016-11-29 2017-05-31 广东海洋大学 A kind of method that utilization egg capsule algae regulates and controls water quality in aquaculture
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CN107603911A (en) * 2017-10-21 2018-01-19 石磊 A kind of preparation method of Composite microbe inocula for aquatic product culture
CN111606426A (en) * 2020-06-08 2020-09-01 济南素帜生物技术有限公司 Preparation method of complex microbial inoculant for aquatic products
CN113016525A (en) * 2020-11-17 2021-06-25 广东海洋大学 Seawater rice planting method for crab-rice symbiotic field
CN112410267A (en) * 2020-12-07 2021-02-26 武汉市鄂正农科技发展有限公司 Microbial inoculum for treating cyanobacterial bloom and preparation method thereof
CN112939164A (en) * 2021-03-15 2021-06-11 广西大学 Novel disinfection method for marine aquaculture water body
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