CN103404716A - Compound microorganism bacterium powder and preparation method thereof - Google Patents
Compound microorganism bacterium powder and preparation method thereof Download PDFInfo
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- CN103404716A CN103404716A CN2013102851935A CN201310285193A CN103404716A CN 103404716 A CN103404716 A CN 103404716A CN 2013102851935 A CN2013102851935 A CN 2013102851935A CN 201310285193 A CN201310285193 A CN 201310285193A CN 103404716 A CN103404716 A CN 103404716A
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Abstract
The invention discloses compound microorganism bacterium powder and a preparation method thereof. The compound microorganism bacterium powder is prepared from the following materials in parts by weight: 20-30 parts of lactobacillus plantarum, 100-200 parts of saccharomycetes, 500-800 parts of molasses, 9000-10000 parts of mineral powder and 1500-2500 parts of high-purity water. The preparation method of the compound microorganism bacterium powder comprises the following steps of uniformly blending the molasses with the high-purity water, and adjusting the temperature to 25 DEG C-30 DEG C; inoculating the lactobacillus plantarum and the saccharomycetes into water in sequence in a compound manner, adjusting the temperature to 25 DEG C-35 DEG C, sealing for fermenting for 5-7 days at the constant temperature; taking and adding spare mineral powder into compound fermented fungicide for stirring, controlling the moisture to 30%-40%, and transferring to a greenhouse and keeping in a dark place for carrying out secondary fermentation for 9-12 days. The compound microorganism bacterium powder can be used for obviously improving the quality of sea cucumbers, improving micro-ecology of a water environment and reducing secondary pollution.
Description
Technical field
The invention belongs to a kind of microbial germ powder, be specifically related to a kind of Lactobacillus plantarum that utilizes and improve culturing sea cucumbers immunity composite microbial bacteria powder and preparation method.
Background technology
Sea cucumber is a kind of famous and precious precious marine product, and in recent years because the market demand is excessively prosperous, the development of holothruian cultures industry is swift and violent.But holothruian cultures also exist larger hidden danger: the one, and the inshore band pollutes day by day serious, and the eutrophication aggravation of seawater, threatening the environment of holothruian cultures; The 2nd, the density of cultivation is more concentrated, and the calamitous disaster of climate reasons takes place frequently in addition, for the control of disease hides some dangers for; The 3rd, natural bait is day by day not enough, and the research of mixed feed is also inadequate, and the holothruian cultures family has little understanding to the compensation process of the feeding mode of sea cucumber and nutrients energy, becomes the bottleneck of sea cucumber bait manufacture and use.Sea cucumber is in propagating environment artificially, its main living environment is positioned at the top layer of bed mud structure, with the feeding method filtered, suck the nutriments such as environmental microorganism albumen in bed mud and cellulose and promote the growth of sea cucumber self, as can be seen here, particularly important to the optimization of the water quality improvement of holothruian cultures and substrate bed mud.
Summary of the invention
In order to address the above problem, the object of the invention is to, a kind of composite microbial bacteria powder and preparation method are provided, utilize Lactobacillus plantarum to optimize intestinal microcirculation in the sea cucumber body, the improvement microorganism, improve the beche-de-mer products quality, improve micro-ecology of water environment, reduce secondary pollution.
Composite microbial bacteria powder provided by the invention, by the raw material of following weight parts, made:
Lactobacillus plantarum 20-30 part, saccharomycete 100-200 part, molasses 500-800 part, mineral powder 9000-10000 part, high purity water 1500-2500 part; Described Lactobacillus plantarum number of viable >=600000cfu/ml, saccharomycete number of viable >=250cfu/ml, the molasses pol is 75 °.
The preparation method of described composite microbial bacteria powder comprises the following steps:
One, allotment nutrient solution: by molasses, high purity water allotment evenly;
Two, Mixed inoculation: adjustment is to 25-30 ℃, successively by Lactobacillus plantarum, saccharomycete Mixed inoculation in nutrient solution;
Three, sealed thermostat fermentation: adjustment is to 25-35 ℃, sealed thermostat fermentation 5-7 days;
Four, secondary fermentation: get the microbial inoculum mix after standby mineral powder adds composite fermentation, moisture is controlled at 30-40%, moves to warm canopy lucifuge secondary fermentation 9-12 days.
The preparation method of described composite microbial bacteria powder, 14-19 days altogether ferments under sealed thermostat condition and lucifuge temperature canopy.
Composite microbial bacteria powder provided by the invention and preparation method, its beneficial effect is, make culturing sea cucumbers fully absorb the nutriment in bait, promote intestinal health and the digestion and absorption function of sea cucumber, obviously improved the quality of sea cucumber, reduced the disease occurrence probability, survival rate is high, case fatality rate is low, micro-ecology of water environment be improved significantly, reduced secondary pollution.
The specific embodiment
Below in conjunction with two embodiment, composite microbial bacteria powder provided by the invention and preparation method are described in detail.
Embodiment 1: the holothruian cultures base
The present embodiment composite microbial bacteria powder, by the raw material of following weight parts, made:
Lactobacillus plantarum 25g, saccharomycete 150g, molasses 600g, mineral powder 9500g, high purity water 1800ml, Lactobacillus plantarum number of viable >=600000cfu/ml wherein, saccharomycete number of viable >=250cfu/ml, the molasses pol is 75 °.
The preparation method of composite microbial bacteria powder, adopt following steps: first by molasses, high purity water allotment evenly, adjustment to 27 ℃, then successively by Lactobacillus plantarum, saccharomycete Mixed inoculation in water, adjustment to 29 ℃, sealed thermostat fermentation 6 days, then get the microbial inoculum mix after standby mineral powder adds composite fermentation, moisture is controlled at 35%, moves to warm canopy lucifuge secondary fermentation 10 days.
Using method: during ,Fan pond, ,Shai pond, clear pond, ore pulvis after the fermentation of Lactobacillus plantarum microorganism is splashed in substrate bed mud top layer.
Embodiment 2: the holothruian cultures aquaculture base
The present embodiment composite microbial bacteria powder, by the raw material of following weight parts, made:
Lactobacillus plantarum 28g, saccharomycete 170g, molasses 700g, mineral powder 9800g, high purity water 2200ml, Lactobacillus plantarum number of viable >=600000cfu/ml wherein, saccharomycete number of viable >=250cfu/ml, the molasses pol is 75 °.
The preparation method of composite microbial bacteria powder, adopt following steps: first by molasses, high purity water allotment evenly, adjustment to 29 ℃, then successively by Lactobacillus plantarum, saccharomycete Mixed inoculation in water, adjustment to 32 ℃, sealed thermostat fermentation 6 days, then get the microbial inoculum mix after standby mineral powder adds composite fermentation, moisture is controlled at 32%, moves to warm canopy lucifuge secondary fermentation 11 days.
Using method: pulvis and bait, feed-mixing is even, throw and be spread in water body.
Claims (3)
1. composite microbial bacteria powder, it is characterized in that: it is made by the raw material of following weight parts:
Lactobacillus plantarum 20-30 part, saccharomycete 100-200 part, molasses 500-800 part, mineral powder 9000-10000 part, high purity water 1500-2500 part; Described Lactobacillus plantarum number of viable >=600000cfu/ml, saccharomycete number of viable >=250cfu/ml, the molasses pol is 75 °.
2. the preparation method of a composite microbial bacteria powder claimed in claim 1, it is characterized in that: it comprises the following steps:
One, allotment nutrient solution: by molasses, high purity water allotment evenly;
Two, Mixed inoculation: adjustment is to 25-30 ℃, successively by Lactobacillus plantarum, saccharomycete Mixed inoculation in nutrient solution;
Three, sealed thermostat fermentation: adjustment is to 25-35 ℃, sealed thermostat fermentation 5-7 days;
Four, secondary fermentation: get the microbial inoculum mix after standby mineral powder adds composite fermentation, moisture is controlled at 30-40%, moves to warm canopy lucifuge secondary fermentation 9-12 days.
3. the preparation method of composite microbial bacteria powder according to claim 2, it is characterized in that: 14-19 days altogether ferments under sealed thermostat condition and lucifuge temperature canopy.
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| CN201310285193.5A CN103404716B (en) | 2013-07-09 | 2013-07-09 | Compound microorganism bacterium powder and preparation method |
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| CN201310285193.5A CN103404716B (en) | 2013-07-09 | 2013-07-09 | Compound microorganism bacterium powder and preparation method |
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| CN103404716A true CN103404716A (en) | 2013-11-27 |
| CN103404716B CN103404716B (en) | 2015-08-19 |
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Cited By (5)
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|---|---|---|---|---|
| CN103820427A (en) * | 2014-03-25 | 2014-05-28 | 钟华 | Preparation method of microbial water quality purifying fungicide |
| CN104431292A (en) * | 2014-11-28 | 2015-03-25 | 靖江哈冠家禽养殖专业合作社 | Process for processing microbial secondary fermentation feed for poultry breeding |
| CN105272769A (en) * | 2015-11-30 | 2016-01-27 | 潍坊泽宇生物科技有限公司 | Composite microbial straw decomposing agent and preparation method thereof |
| CN106754514A (en) * | 2016-12-23 | 2017-05-31 | 钟华 | A kind of sustainable desilting is except the microbial bacterial agent preparation method of black and odorous water |
| TWI655172B (en) * | 2017-08-22 | 2019-04-01 | 禾畚興業有限公司 | Plant waste reuse technology |
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| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103820427A (en) * | 2014-03-25 | 2014-05-28 | 钟华 | Preparation method of microbial water quality purifying fungicide |
| CN104431292A (en) * | 2014-11-28 | 2015-03-25 | 靖江哈冠家禽养殖专业合作社 | Process for processing microbial secondary fermentation feed for poultry breeding |
| CN105272769A (en) * | 2015-11-30 | 2016-01-27 | 潍坊泽宇生物科技有限公司 | Composite microbial straw decomposing agent and preparation method thereof |
| CN106754514A (en) * | 2016-12-23 | 2017-05-31 | 钟华 | A kind of sustainable desilting is except the microbial bacterial agent preparation method of black and odorous water |
| CN106754514B (en) * | 2016-12-23 | 2019-08-13 | 钟华 | A kind of sustainable dredging removes the microbial bacterial agent preparation method of black and odorous water |
| TWI655172B (en) * | 2017-08-22 | 2019-04-01 | 禾畚興業有限公司 | Plant waste reuse technology |
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| CN103404716B (en) | 2015-08-19 |
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