CN103039722B - Active biological composite bait for marine animals and preparation method thereof - Google Patents
Active biological composite bait for marine animals and preparation method thereof Download PDFInfo
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- CN103039722B CN103039722B CN201210515149.4A CN201210515149A CN103039722B CN 103039722 B CN103039722 B CN 103039722B CN 201210515149 A CN201210515149 A CN 201210515149A CN 103039722 B CN103039722 B CN 103039722B
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Abstract
The invention discloses active biological composite bait for marine animals. The bait is prepared from the following components: 1,200 grams of nannochloris oculata paste with the density of 40 billion colony forming units (CFU) per gram, 750 billion CFU of rhodotorula benthica, 200 billion CFU of rhodopuesdomonas pulstris, 400 billion CFU of bacillus subtilis, 120 billion CFU of acetic acid bacteria, 800 billion CFU of bifidobacteria, 40 billion CFU of actinomycetes, 105 billion CFU of lactic acid bacteria, 150 billion CFU of nitrobacteria, 100 grams of zeolite powder, 750 milligrams of vitamin B1, 7.5 milligrams of vitamin B12, 12,500 milligrams of vitamin C, 50 milligrams of vitamin H, and 200 milliliters of boiled seawater. A preparation method sequentially comprises the following steps of: separating nannochloris oculata from a culture solution, concentrating the nannochloris oculata to obtain the nannochloris oculata paste with the density of 40 billion cells per gram, and irradiating the paste for 6 hours under an ultraviolet lamp; and adding the other raw materials of the calculated quantity into a stirrer, and stirring and mixing uniformly.
Description
Technical field
The present invention relates to a kind of marine animal bait, especially can meet completely marine animal to the demand of nutriment, improve micro-ecological environment in cultivated animals body, improve individual immunity power and a survival rate, active bio composite bait and preparation method thereof for marine animal of the harmful chemical in degradable water, purifying aquaculture water environment simultaneously.
Background technology
In the past, in the marine animal processes such as industrial aquaculture shellfish, echinoderm, fish, shrimp and crab class, need a large amount of marine microalgaes (diatom, green alga, chrysophyceae etc.), as the direct bait of the shellfish as filter-feeding, echinoderm, as the bait of the edible zooplankter of fish, shrimp and crab class.Yet, the cultivation cycle of marine microalgae is longer, from one-level, cultivate level Four cultivation and approximately need about one month, and require high to condition of culture, careless slightly pollution in wide area and the very difficult recovery at short notice that will cause cultivating water body, meanwhile, the nutrient solution of marine microalgae contains this raw metabolite of a large amount of algae, without any processing, just together with micro-algae, throw something and feed, for seed, there is very high intoxicating.The existing the problems referred to above of marine microalgae cause degradation under seedling body subalimentation, poor growth and premunition, so that seedling body production declining, cause great economic loss to the unit of growing seedlings.
For the problems referred to above, the unit of growing seedlings having adopts bacterium, yeast and microparticle artifical compound feed to substitute marine microalgae, but these alternative food values are lower, and in water body, conformality is poor, affect marine animal edible, but also can cause serious pollution to water environment.Especially when high cultivation density, high daily ration, feeding quantity, often cause that cultivation water environment ammonia nitrogen exceeds standard, water quality deterioration, and then cause the problems such as the pathogenic microorganisms such as bacterium and virus grow in a large number, had a strong impact on seed output and quality.Although can suppress to a great extent the outburst of pathogenic microorganisms by throwing in the antibiotic medicines such as erythromycin, streptomysin, cephalo, high-caliber medicament residue exists great food security hidden danger.
Summary of the invention
The present invention is in order to solve existing the problems referred to above in prior art, and active bio composite bait and preparation method thereof for a kind of marine animal is provided.
Technical solution of the present invention is: a kind of marine animal active bio composite bait, is characterized in that by following component formulated in proportion: 40,000,000,000 cells of density/gram 1200 grams of Nannochloropsis oculata algae cream, ocean rhodotorula 7,500 hundred million CFU, Rhodopseudomonas palustris 2,000 hundred million CFU, bacillus subtilis 4,000 hundred million CFU, acetic acid bacteria 1,200 hundred million CFU, Bifidobacterium 8,000 hundred million CFU, actinomyces 40,000,000,000 CFU, lactic acid bacteria 1,050 hundred million CFU, nitrobacteria 1,500 hundred million CFU, 100 grams of zeolite powders, Cobastab
1750 milligrams, Cobastab
127.5 milligrams, 12500 milligrams of vitamin Cs, 50 milligrams of biotins, boil 200 milliliters, seawater.
A preparation method for active bio composite bait for above-mentioned marine animal, is characterized in that carrying out as follows successively:
A. Nannochloropsis oculata is separated with nutrient solution, and be concentrated into density be 40,000,000,000 cells/gram Nannochloropsis oculata algae cream, algae cream is placed under uviol lamp and is irradiated 6 hours;
B. by Nannochloropsis oculata algae cream, ocean rhodotorula, Rhodopseudomonas palustris, bacillus subtilis, acetic acid bacteria, Bifidobacterium, actinomyces, lactic acid bacteria, nitrobacteria, zeolite powder, the Cobastab of amount of calculation
1, Cobastab
12, vitamin C, biotin and boil seawater and join in mixer, be uniformly mixed.
The present invention selects and is easy to High Density Cultivation and collects facility, after multigelation, do not decompose conformality better, the seawater Nannochloropsis oculata that protein and high unsaturated fatty acid content are higher (a kind of unicellular micro-algae) is as primary raw material, can improve the micro-ecological environment in cultivated animals body, while degradation water environment ammonia nitrogen, nitrous acid nitrogen, the noxious materials such as hydrogen sulfide, the probio of improving quality of water environment is auxiliary element, also select be simultaneously rich in aluminosilicate and trace element zeolite powder and polytype vitamin as additive, with the cleaning-sterilizing seawater of salinity 15ppt to raw material, auxiliary material and additive mix, the biological composite bait body of paste of moulding one-tenth marine active.Each component interaction, can meet completely marine animal to the demand of nutriment, improve micro-ecological environment in cultivated animals body, improve individual immunity power and survival rate, harmful chemical, the purifying aquaculture water environment in degradable water simultaneously.Can be used as the vegetalitas living body bait of shellfish, echinoderm and zooplankter, for the population breeding of the bait such as the parent accelerating of marine products economic animal scallop, clam, Bao, sea cucumber, sea urchin and larval culture and zooplankton rotifer, cladocera and Copepods provides clean, stable, reliably and the food resource of high-quality, reduce antibiotic dosage, stop medicament residue.
The specific embodiment
A. by channel separator, by Nannochloropsis oculata separated with nutrient solution and be concentrated into density be 40,000,000,000 cells/gram Nannochloropsis oculata algae cream, algae cream is placed under uviol lamp and irradiates 6 hours, to kill bacterium and protozoic live body and the packing of sneaking in Nannochloropsis oculata algae cream;
B. to adding following composition in mixer: 1200 grams of Nannochloropsis oculata algae cream (40,000,000,000 cell/gram), 150 grams, ocean rhodotorula powder (specification 5,000,000,000 CFU/ gram), 40 grams, Rhodopseudomonas palustris powder (specification 5,000,000,000 CFU/ gram), 20 grams, bacillus subtilis powder (specification 20,000,000,000 CFU/ gram), 40 grams, acetic acid bacteria powder (specification 3,000,000,000 CFU/ gram), 40 grams, Bifidobacterium powder (specification 20,000,000,000 CFU/ gram), 40 grams, actinomyces powder (specification 1,000,000,000 CFU/ gram), 35 grams, lactic acid bacteria powder (specification 3,000,000,000 CFU/ gram), 50 grams, nitrobacteria powder (specification 3,000,000,000 CFU/ gram), 100 grams of zeolite powders (specification 200 orders), Cobastab
1(specification 50 mg/ml) 15 milliliters, Cobastab
1225 of (specification 0.5 mg/ml) 15 milliliters, vitamin Cs (specification 500 millis gram/piece), 10 of biotins (specification 5 millis gram/piece), boil 200 milliliters, seawater (salinity is 15ppt), stir and mix for 3 ~ 5 minutes, pack grinding tool into moulding, and be placed in-18 ℃ of freezing preservations.
Prepared marine animal active bio composite bait, formulated in proportion by following component: 40,000,000,000 cells of density/gram 1200 grams of Nannochloropsis oculata algae cream, ocean rhodotorula 7,500 hundred million CFU, Rhodopseudomonas palustris 2,000 hundred million CFU, bacillus subtilis 4,000 hundred million CFU, acetic acid bacteria 1,200 hundred million CFU, Bifidobacterium 8,000 hundred million CFU, actinomyces 40,000,000,000 CFU, lactic acid bacteria 1,050 hundred million CFU, nitrobacteria 1,500 hundred million CFU, 100 grams of zeolite powders, Cobastab
1750 milligrams, Cobastab
127.5 milligrams, 12500 milligrams of vitamin Cs, 50 milligrams of biotins, boil 200 milliliters, seawater.
During use, in normal temperature, thaw, be placed in the bolting silk net in 30 ~ 60 microns, aperture, evenly rub in seawater, splash and throw something and feed.
Raw material sources:
| Material name | Specification | Source |
| Nannochloropsis oculata | 40000000000 cells/gram | Upper sea light language bio tech ltd |
| Ocean rhodotorula powder | 5000000000 CFU/ gram | Cangzhou City Fang Yuan bioengineering Co., Ltd |
| Rhodopseudomonas palustris powder | 5000000000 CFU/ gram | Jiangsu Lv Ke Bioisystech Co., Ltd |
| Bacillus subtilis powder | 20000000000 CFU/ gram | Jiangsu Lv Ke Bioisystech Co., Ltd |
| Acetic acid bacteria powder | 3000000000 CFU/ gram | Shaanxi Times Brewing Technology Co., Ltd. |
| Bifidobacterium powder | 20000000000 CFU/ gram | Neck THKnet is won (Beijing) scientific & trading Co., Ltd. |
| Actinomyces powder | 1000000000 CFU/ gram | Jin Lifeng biotech inc, Shandong |
| Lactic acid bacteria powder | 3000000000 CFU/ gram | Neck THKnet is won (Beijing) scientific & trading Co., Ltd. |
| Nitrobacteria powder | 3000000000 CFU/ gram | Cangzhou City Fang Yuan bioengineering Co., Ltd |
Zeolite powder, Cobastab
1, Cobastab
12, vitamin C, biotin be the product of public offering on market.
Embodiment of the present invention effect is compared with the prior art as follows:
(1) the sea cucumber young (body 1.2 ~ 1.6 centimetres in length) cultivation period, the application embodiment of the present invention and commercially available three kinds of sea cucumber compound feeds contrast: water body ammonia-nitrogen content 0.010 ~ 0.017 mg/litre of the embodiment of the present invention, the water body ammonia-nitrogen content of commercially available sea cucumber compound feed reaches 0.97 ~ 1.83 mg/litre, and water body ammonia-nitrogen content declines 90% ~ 95%; The water nitrite acid nitrogen of the embodiment of the present invention of throwing something and feeding is 0.0011 ~ 0.0023 mg/litre, and the water nitrite acid nitrogen content of commercially available sea cucumber compound feed reaches 0.42 ~ 0.71 mg/litre, and water nitrite acid nitrogen content declines and surpasses 95%.Cultivate after 30 days, the sea cucumber larvae survival rate of the embodiment of the present invention of throwing something and feeding is 87.5%, and rate of body weight gain is 237%; The sea cucumber larvae survival rate of commercially available mixed feed is only 43.1%, and rate of body weight gain is 75%, and the embodiment of the present invention has significant facilitation effect to the survival rate of the sea cucumber young and rate of body weight gain.
(2) brachionus plicatilis high-density culture process, the chlorella that the embodiment of the present invention and batch production are cultivated, salt algae and equilateral chrysophyceae application contrast: while throwing something and feeding inventive embodiments, brachionus plicatilis population culture density can reach 3000 ~ 5000/milliliter, and the live body marine microalgae population density of throwing something and feeding only reaches 50 ~ 70/milliliter, wheel animalcule culture efficiency exceeds 60 ~ 100 times than live body marine microalgae.The brachionus plicatilis protein content of the embodiment of the present invention of throwing something and feeding is 48.5%, DHA is 1.2%, EPA is 13.8%, the throw something and feed wheel animalcule protein content 35.7 ~ 49.2% of marine microalgae, DHA is 0.78 ~ 1.77%, EPA is 6.87 ~ 11.8%, and the wheel animalcule nutrition content that the visible embodiment of the present invention is cultivated is equal to the wheel animalcule that live body marine microalgae is cultivated.
(3) Patinopecten yessoensis larval culture period, the mixed algae liquid application contrast of chlorella, salt algae, equilateral chrysophyceae and Chaetoceros that the embodiment of the present invention and batch production are cultivated: the embodiment of the present invention of throwing something and feeding is after 12 days, the settlement and metamorphosis rate of the Patinopecten yessoensis young is 91.3%, after 30 days, juvenile mollusk shell is long 120 ~ 125 millimeters, seedling water total nitrogen content is 0.13 mg/litre, and total phosphorus content is 0.07 mg/litre; The Micro Algae nutrient solution (micro-algae without cross concentration) of throwing something and feeding, after the embodiment of the present invention of throwing something and feeding 12 days, the settlement and metamorphosis rate of the Patinopecten yessoensis young is that after 68.9%, 30 day, juvenile mollusk shell is grown 55 ~ 75 millimeters, seedling water total nitrogen content is 1.44 mg/litre, and total phosphorus content is 0.88 mg/litre.Compare with conventional marine microalgae, while using the embodiment of the present invention, the distortion ratio of the Patinopecten yessoensis young significantly improves, and individual growth speed increases nearly 1 times; In addition, effectively controlled and thrown something and fed marine microalgae and with algae liquid, bring the content of nitrogen and phosphorous of growing seedlings in water environment into, quality of water environment is significantly better than the experimental group of the marine microalgae mixed liquor of throwing something and feeding.
(4) the sea cucumber young (body 1.2 ~ 1.6 centimetres in length) cultivation period, the application embodiment of the present invention and commercially available three kinds of probioticses contrast: sea cucumber larval culture water body ammonia-nitrogen content 0.013 ~ 0.022 mg/litre of the embodiment of the present invention, using the water body ammonia-nitrogen content of probiotics is 0.17 ~ 0.23 mg/litre, and the water body ammonia-nitrogen content of the application embodiment of the present invention declines nearly 10 times than the commercially available probiotics of application.Nitrous acid nitrogen content, the water body of the embodiment of the present invention of throwing something and feeding is 0.0019 ~ 0.0033 mg/litre, using the water nitrite acid nitrogen content of probiotics is 0.009 ~ 0.011 mg/litre, and the water nitrite acid nitrogen content suppression ratio of the application embodiment of the present invention is applied commercially available probiotics and declined nearly 5 times.
Claims (2)
1. a marine animal active bio composite bait, is characterized in that by following component formulated in proportion: density 40,000,000,000 cells/gram 1200 grams of Nannochloropsis oculata algae cream, ocean rhodotorula 7,500 hundred million CFU, Rhodopseudomonas palustris 2,000 hundred million CFU, bacillus subtilis 4,000 hundred million CFU, acetic acid bacteria 1,200 hundred million CFU, Bifidobacterium 8,000 hundred million CFU, actinomyces 40,000,000,000 CFU, lactic acid bacteria 1,050 hundred million CFU, nitrobacteria 1,500 hundred million CFU, 100 grams of zeolite powders, Cobastab
1750 milligrams, Cobastab
127.5 milligrams, 12500 milligrams of vitamin Cs, 50 milligrams of biotins, boil 200 milliliters, seawater.
2. a preparation method for active bio composite bait for marine animal as claimed in claim 1, is characterized in that carrying out as follows successively:
A. Nannochloropsis oculata is separated with nutrient solution, and be concentrated into density be 40,000,000,000 cells/gram Nannochloropsis oculata algae cream, algae cream is placed under uviol lamp and is irradiated 6 hours;
B. by Nannochloropsis oculata algae cream, ocean rhodotorula, Rhodopseudomonas palustris, bacillus subtilis, acetic acid bacteria, Bifidobacterium, actinomyces, lactic acid bacteria, nitrobacteria, zeolite powder, the Cobastab of amount of calculation
1, Cobastab
12, vitamin C, biotin and boil seawater and join in mixer, be uniformly mixed.
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| Application Number | Priority Date | Filing Date | Title |
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| CN201210515149.4A CN103039722B (en) | 2012-12-05 | 2012-12-05 | Active biological composite bait for marine animals and preparation method thereof |
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| CN201210515149.4A CN103039722B (en) | 2012-12-05 | 2012-12-05 | Active biological composite bait for marine animals and preparation method thereof |
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| CN103039722A CN103039722A (en) | 2013-04-17 |
| CN103039722B true CN103039722B (en) | 2014-07-16 |
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| Publication number | Priority date | Publication date | Assignee | Title |
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| CN104445630A (en) * | 2013-09-13 | 2015-03-25 | 领绿生物镇江有限公司 | Microbial preparation with water purification and growth promotion dual efficacies, and preparation method thereof |
| CN103535301B (en) * | 2013-10-23 | 2015-04-22 | 烟台海融电力技术有限公司 | Method for cultivating sea cucumber larvae |
| CN105433050A (en) * | 2015-12-16 | 2016-03-30 | 苏州蟹家族生态农业有限公司 | High-nutrition crab feed and preparation method thereof |
| CN107535677A (en) * | 2017-09-13 | 2018-01-05 | 威海海洋职业学院 | A kind of feed addictive for improving young Bao survival rate and preparation method thereof |
| CN110257292B (en) * | 2019-06-27 | 2022-11-15 | 中国科学院烟台海岸带研究所 | Microbial agent for aquaculture and preparation method thereof |
| CN111670868B (en) * | 2020-06-15 | 2022-03-11 | 珠海麦得发生物科技股份有限公司 | PHA-rich sea cucumber bait |
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