CN102389038A - Production process of microorganism additive for Chinese softshell turtle - Google Patents
Production process of microorganism additive for Chinese softshell turtle Download PDFInfo
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- CN102389038A CN102389038A CN2011103337475A CN201110333747A CN102389038A CN 102389038 A CN102389038 A CN 102389038A CN 2011103337475 A CN2011103337475 A CN 2011103337475A CN 201110333747 A CN201110333747 A CN 201110333747A CN 102389038 A CN102389038 A CN 102389038A
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
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Abstract
The invention discloses a production process of microorganism additive for Chinese softshell turtle, comprising the following process steps: firstly selecting bacillus subtilis, nitrobacteria, denitrifying bacteria and actinomycetes as strains; secondly, adding 400kg of cleaning tap water in a reactor, sterializing for 60min, then stirring and raising the temperature; thirdly, taking 10kg of brown sugar and 15kg of peptone, dissolving the brown sugar and peptone in warm water, then adding in the reactor, sterilizing for 60min, then stirring for raising the temperature to be more than 30 DEG C; fourthly dissolving the strains in warm water with the temperature being 40 DEG C, then adding the strains in the reactor, and under the stirring state, culturing the strains for 48h at the temperature being 35 DEG C; fifthly, when the micropropagation condition is checked to be good, adding strain partners, stirring for 15min, and then checking the growth condition; and sixthly, packaging and weighing. The production process is not only simple in operation, bus also easy to promote, and has good using effect.
Description
Technical field
The present invention relates to a kind of production technology of Chinese soft-shelled turtle microorganism fodder additive.
Background technology
Microbe additive (Microecological agent) is claimed microecologic regulator (Microecological modulator), probiotic (Probiotic) etc. again; Be meant under little ecological theory instructs; Use little ecological principle, utilize biologic product or active bacteria formulation that the normal microorganism or the normal microorganism growth promoting substance of the useful and harmless work of host are processed through special process such as cultivation, fermentation, dryings.It has disease preventing and treating, enhancing body immunity, promote growth, optimize multiple function such as water body, and pollution-free, noresidue, does not develop immunity to drugs.In recent years, domestic had much about the application of microbe additive and the report of research aspect, but little ecological technique of China also is in starting, and many The Application of Technology await further to study and explore.
Along with the development of aquaculture, the application of mixed feed and feed addictive is more and more wider, and various types of growth accelerators arise at the historic moment with other antibiotics additives.Some drugs has certain effect at aspects such as promoting growth, raising efficiency of feed utilization and disease control really, but also brings the drawback of product quality safety.At present, little ecological technique is day by day ripe, has brought hope for solving the above-mentioned drawback that exists in the aquaculture.
At present, the agricultural product ubiquity of China subject matters such as drug residue height, poor quality.The abuse of antibiotic medicine in breeding process such as livestock and poultry, aquatic products adds phenomenon ubiquities such as growth accelerator, hormone in the feed.Though accelerated the speed of growth of animal, quality produced negative effect.At first be to have destroyed the intestinal microecology balance, cause body that the neurological susceptibility of pathogenic microorganism is raise, accumulating of antibiotic generation and antibiotic content causes the harm to human health, and this has become great public health problem.Utilize not contain hazardous substance, have no side effect, free from environmental pollution, and can promote the cultivated animals growth, and improving the microbe additive of immunity of organisms, the pollution-free food of producing safety and Health becomes important research project of the present invention.
Summary of the invention
The object of the present invention is to provide a kind of production technology of Chinese soft-shelled turtle microorganism fodder additive.Not only simple to operate being easy to of the present invention promoted, and result of use is good.
In order to reach above-mentioned purpose, solution of the present invention is:
A kind of production technology of Chinese soft-shelled turtle microorganism fodder additive comprises following processing step: 1) select bacillus subtilis, nitrobacteria, denitrifying bacteria, actinomyces (purchasing in Puren Ecology Tech, Co., Ltd., Beijing) as bacterial classification; 2) in reactor, add 400 ㎏ cleaning running water, stir behind the sterilization 60min and heat up; 3) get brown sugar 10 ㎏, peptone 15 ㎏, warm water melts the back and adds reactor, stirs behind 60 min that sterilize again to be warming up to more than 30 ℃; 4) with 40 ℃ of warm water bacterial classification is melted after, add reactor, under stirring, temperature is 35 ℃ and cultivates 48h; 5) numerous all right back adding bacterial classification companion is expanded in inspection, and checks growing state after stirring 15min; 6) after the week, numerous situation is expanded in inspection, expands numerously well can use.
The adding weight ratio of bacterial classification is in the described step 4): bacillus subtilis: nitrobacteria: denitrifying bacteria: actinomyces=1:2:2:1.
Expand numerous situation in the described step 6) and be meant bacterial classification in aerobic environment, temperature keeps breeding under 35 ℃ the condition and growing the process that flora quantity increases fast; Adopt microscope, blood counting chamber instrument, observe spawn activity and unit bacterial classification quantity; Energetic, bacterial classification quantity>=1.0 * 10
6Individual/ml, satisfy above two conditions, be regarded as expanding numerous good; Both lack one and are regarded as expanding numerous bad, and it is numerous to carry out the expansion in a week again.
Beneficial effect of the present invention is: the present invention is on the basis of the physiology rerum natura, eucaryotic cell structure, composition and the secretory product thereof that have taken into full account bacterial classification; Chosen and do not had mutually exclusive or antagonism between each bacterial strain, and had mutual synergistic micro-ecological composite bacterium.Not only simple to operate being easy to of the present invention promoted; And result of use is good; Breeding water body had good optimization improvement effect; For improving the water body dissolved oxygen amount, stablize water body pH and change, ammonia nitrogen, content of nitrite in the reduction water reduce ammonia and nitrite the toxic action of cultivated animals etc. are had positive effect.
Description of drawings
Fig. 1 is a process chart of the present invention;
Fig. 2 is an embodiment of the invention test tank ammonia nitrogen change curve;
Fig. 3 is test tank and contrast pond nitrite change curve;
Fig. 4 is test tank and contrast pond pH value change curve;
Fig. 5 is test tank and contrast pond dissolved oxygen change curve.
The specific embodiment
The production technology of a kind of Chinese soft-shelled turtle microorganism fodder additive of present embodiment; As shown in Figure 1, comprise following processing step: 1) select bacillus subtilis, nitrobacteria, denitrifying bacteria, actinomyces (purchasing) as bacterial classification in Puren Ecology Tech, Co., Ltd., Beijing.2) in reactor, add 400 ㎏ cleaning running water, stir behind the sterilization 60min and heat up.3) get brown sugar 10 ㎏, peptone 15 ㎏, warm water melts the back and adds reactor, stirs behind 60 min that sterilize again to be warming up to more than 30 ℃.4) with 40 ℃ of warm water bacterial classification is melted after, add reactor, under stirring, temperature is 35 ℃ and cultivates 48h; The adding weight ratio of bacterial classification is in the described step 4): bacillus subtilis: nitrobacteria: denitrifying bacteria: actinomyces=1:2:2:1.5) numerous all right back adding bacterial classification companion is expanded in inspection, and checks growing state after stirring 15min; 6) after the week, numerous situation is expanded in inspection, expands numerously well can use.
Expand numerous situation in the described step 6) and be meant bacterial classification in aerobic environment, temperature keeps breeding under 35 ℃ the condition and growing the process that flora quantity increases fast; Adopt microscope, blood counting chamber instrument, observe spawn activity and unit bacterial classification quantity; Energetic, bacterial classification quantity>=1.0 * 10
6Individual/ml, satisfy above two conditions, be regarded as expanding numerous good; Both lack one and are regarded as expanding numerous bad, and it is numerous to carry out the expansion in a week again.
The application of microbe additive in breed
1, experimental condition
Carry out in the screening pond of test and Selection in the national Chinese soft-shelled turtle seed farm in Shaoxing, and test is provided with 3 test tanks, 1 contrast pond, and every pond is equipped with independently inlet pipe, drain pipe road.Four ponds at area, the depth of water, aquaculture model, to put aspects such as quantity, auxiliary facility, feeding and management in a suitable place to breed all identical.Test tank is as shown in table 1 with contrast pond concrete condition.
2, test method
Test period is that 200X June 11 was to July 11,1 month by a definite date.Test uses 3 experiment pool as repetition, and 1 pond is as contrast.Area is 153m
2, 1800 of soft-shelled turtles are put in every pond, about body weight 200 grams.Microbe additive is every, and 3 experiment pool are respectively 5 ppm, 10 ppm, 20 ppm at a distance from 15 days full pool spilling heads 1 time, and contrasting the pond is 0 ppm.Whenever measured and write down following project at a distance from 3 days: weather, water temperature, ammonia nitrogen, nitrite, pH value, dissolved oxygen.The each detection by the special messenger fixes a point to gather
3, result of the test and analysis
(1) to the analysis of ammonia nitrogen effect
The change curve of ammonia nitrogen shows from the data measured and water body: the use of microbe additive is not The more the better, has only certain application dosage just can receive the effect of removing ammonia nitrogen preferably, and dosage too much or all undesirable very little.Test No. 1 pond because using dosage is less, and the microbe additive absolute predominance is shorter relatively, therefore can not be lasting to the transformation time of ammonia; Test No. 3 ponds because application dosage is high, the nitrogen element in the more culture medium is got in the water body, cause ammonia content to increase; Therefore, No. 1, No. 3 variations of test ammonia content alters a great deal.The contrast pond almost has no change.After testing No. 2 pond ammonia and being transformed, long term maintenance is a lower level, and effect is fairly obvious.The concrete result that measures sees table 2 and Fig. 2; Table 2 is test tank and contrast pond ammonia nitrogen determination record sheet; Fig. 2 is test tank and contrast pond ammonia nitrogen change curve.
(2) to the analysis of nitrite effect
Table 3 is that test tank is measured record with contrast pond nitrite; Fig. 3 is test tank and contrast pond nitrite change curve; From the nitrite change curve, can find out: along with microbe additive strengthens to the ability that nitrite transforms ammonia, the content of nitrosonium salts increases gradually in the water body, but along with microbe additive continues nitrosonium salts is transformed, its content progressively descends again.But because No. 1 pond application dosage of test is less, the microbe additive total bacteria count is in the decline phase in the later stage water body, therefore tests No. 1 pond nitrite and alters a great deal.Compare with the contrast pond and test No. 2 ponds, then be in low-level state all the time.Test No. 3 ponds because dosage is too big, then oxygen demand increases, and therefore ammonia and nitrite is transformed, and is not very good.
(3) to the analysis of pH value effect
Table 4 is test tank and contrast pond pH pH-value determination pH record; Fig. 4 is test tank and contrast pond pH value change curve; From the data that record and curve, find out: it mainly is due to the photosynthesis of algae in the water body that pH changes, use microbe additive after, algae and microorganism have formed competition, and microorganism is tending towards absolute predominance.Therefore, the water pH value amplitude of variation is little in 3 test tanks, and contrast pond amplitude of variation is bigger.This shows the effect that microbe additive has stable water body pH to change.
(4) to the function analysis of dissolved oxygen
Table 5 is test tank and contrast pond DO determination record, and Fig. 5 is test tank and contrast pond dissolved oxygen change curve; Can be clear that from Fig. 5 and table 5 test tank and the order that increases successively of contrast pond dissolved oxygen are: test No. 2 ponds, No. 1 pond of test, No. 3 ponds of test, contrast pond, analysis draws in view of the above: microbe additive has stronger degradation capability to COD in water body and BOD.Because No. 3 pond application dosages of test are excessive, cause the oxygen consumption of thalline own to increase, though use in the water body oxygen consumption factor such as organic matter to reduce, in the water body dissolved oxygen amount increase effect does not have preceding two groups desirable.Make that oxygen consumption organic significantly reduces in the water body and test No. 1, No. 2 ponds, thereby improved the dissolved oxygen in the water body.
4, conclusion
Test result analysis through to four aspects of test tank water body can draw: the complex microorganism additive of present embodiment has good optimization improvement effect to breeding water body; For improving the water body dissolved oxygen amount, stablizing water body PH variation; Reduce ammonia nitrogen, content of nitrite in the water, reduce ammonia and nitrite the toxic action of cultivated animals etc. is had positive effect.
Using resultant effect from microbe additive finds out: application dosage can not be excessive can not be too little; Its application process should be breed object and the water body situation that is directed against not, selects suitable application dosage and time, and adheres to chronic administration; Can not be eager for instant success and quick profits, otherwise can run counter to desire.
Claims (3)
1. the production technology of a Chinese soft-shelled turtle microorganism fodder additive is characterized in that comprising following processing step: 1) select bacillus subtilis, nitrobacteria, denitrifying bacteria, actinomyces as bacterial classification; 2) in reactor, add 400 ㎏ cleaning running water, stir behind the sterilization 60min and heat up; 3) get brown sugar 10 ㎏, peptone 15 ㎏, warm water melts the back and adds reactor, stirs behind 60 min that sterilize again to be warming up to more than 30 ℃; 4) with 40 ℃ of warm water bacterial classification is melted after, add reactor, under stirring, temperature is 35 ℃ and cultivates 48h; 5) numerous all right back adding bacterial classification companion is expanded in inspection, and checks growing state after stirring 15min; 6) be Chinese soft-shelled turtle microorganism fodder additive after packing is weighed, subsequent use.
2. the production technology of a kind of Chinese soft-shelled turtle microorganism fodder additive as claimed in claim 1 is characterized in that: the adding weight ratio of bacterial classification is in the described step 4): bacillus subtilis: nitrobacteria: denitrifying bacteria: actinomyces=1:2:2:1.
3. the production technology of a kind of Chinese soft-shelled turtle microorganism fodder additive as claimed in claim 1; It is characterized in that: expand numerous situation in the described step 6) and be meant that bacterial classification is in aerobic environment; Temperature keeps breeding under 35 ℃ the condition and growing the process that flora quantity increases fast; Adopt microscope, blood counting chamber instrument, observe spawn activity and unit bacterial classification quantity; Energetic, bacterial classification quantity>=1.0 * 10
6Individual/ml, satisfy above two conditions, be regarded as expanding numerous good; Both lack one and are regarded as expanding numerous bad, and it is numerous to carry out the expansion in a week again.
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| Application Number | Priority Date | Filing Date | Title |
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| CN2011103337475A CN102389038A (en) | 2011-10-28 | 2011-10-28 | Production process of microorganism additive for Chinese softshell turtle |
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| CN2011103337475A CN102389038A (en) | 2011-10-28 | 2011-10-28 | Production process of microorganism additive for Chinese softshell turtle |
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103039722A (en) * | 2012-12-05 | 2013-04-17 | 大连海洋大学 | Active biological composite bait for marine animals and preparation method thereof |
| CN104221974A (en) * | 2014-10-14 | 2014-12-24 | 石日福 | Shrimp culturing method |
Citations (3)
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| CN101157892A (en) * | 2007-03-06 | 2008-04-09 | 扬州市绿科生物技术有限公司 | Production method of solid preparation for degradation of ammonia nitrogen and nitrite |
| CN101878749A (en) * | 2010-06-11 | 2010-11-10 | 杭州萧山天福生物科技有限公司 | Ecological controllable healthy cultivation method for pelodiscus sinensis |
| CN102106482A (en) * | 2011-01-27 | 2011-06-29 | 绍兴中亚工贸园有限公司 | Feed for culturing soft-shelled turtle and processing technology thereof |
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2011
- 2011-10-28 CN CN2011103337475A patent/CN102389038A/en active Pending
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN101157892A (en) * | 2007-03-06 | 2008-04-09 | 扬州市绿科生物技术有限公司 | Production method of solid preparation for degradation of ammonia nitrogen and nitrite |
| CN101878749A (en) * | 2010-06-11 | 2010-11-10 | 杭州萧山天福生物科技有限公司 | Ecological controllable healthy cultivation method for pelodiscus sinensis |
| CN102106482A (en) * | 2011-01-27 | 2011-06-29 | 绍兴中亚工贸园有限公司 | Feed for culturing soft-shelled turtle and processing technology thereof |
Non-Patent Citations (2)
| Title |
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| 蒋平梁: "无公害中华鳖生态养殖及养殖污水综合处理_零排放_集成技术", 《杭州农业科技》 * |
| 高培国等: "中华鳖生态混养技术研究", 《科学养鱼》 * |
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103039722A (en) * | 2012-12-05 | 2013-04-17 | 大连海洋大学 | Active biological composite bait for marine animals and preparation method thereof |
| CN103039722B (en) * | 2012-12-05 | 2014-07-16 | 大连海洋大学 | Active biological composite bait for marine animals and preparation method thereof |
| CN104221974A (en) * | 2014-10-14 | 2014-12-24 | 石日福 | Shrimp culturing method |
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Application publication date: 20120328 |