CN104030459A - Microbial preparation for brackish water aquaculture, and preparation method and application thereof - Google Patents

Microbial preparation for brackish water aquaculture, and preparation method and application thereof Download PDF

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Publication number
CN104030459A
CN104030459A CN201410286081.6A CN201410286081A CN104030459A CN 104030459 A CN104030459 A CN 104030459A CN 201410286081 A CN201410286081 A CN 201410286081A CN 104030459 A CN104030459 A CN 104030459A
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China
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microbial preparation
preparation
aquaculture
brackish water
sodium
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CN201410286081.6A
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Chinese (zh)
Inventor
袁春营
王婉莹
杨硕
崔青曼
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Tianjin University of Science and Technology
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Tianjin University of Science and Technology
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Abstract

The invention relates to a microbial preparation for brackish water aquaculture, which comprises the following components in parts by weight: 20-25 parts of Bacillus subtilis, 10-20 parts of Bacillus megaterium, 20-25 parts of microzyme, 30-35 parts of Acinetobacter and 100-200 parts of carrier. The preparation method comprises the following steps: sequentially inoculating Bacillus subtilis, Bacillus megaterium, microzyme and Acinetobacter seed culture solutions into a culture medium, fermenting, and mixing the obtained bacterium solution with the carrier substance to obtain the microbial preparation. The microbial preparation is applicable to a culture water body with the salinity range of 0.2-1%. The microbial preparation can obviously improve the water quality of the brackish water aquaculture water body, especially obviously lowers the nitrite content in the water body, and can enhance the flesh texture of the cultured animals.

Description

Microbial preparation and preparation method and application for a kind of brackish water aquaculture
Technical field
The invention belongs to microbial preparation technical field, microbial preparation and preparation method and application for especially a kind of brackish water aquaculture.
Background technology
Nitrobacteria (Nitrite-oxidizing bacteria, NOB) be the microorganism species that a large class plays a crucial role in nature Nitrogen Cycling, but due to they special ecological physiology attributes, poor growth, mean generation time is in about 10h, and is easily subject to the impact of external environment, and its application is restricted, and the problem of current Chinese brackish water aquaculture polluted by nitrogen is on the rise, in the urgent need to carrying out the improvement of polluted by nitrogen.
Because China's brackish water aquaculture industry development is rapid, cultivation density improves constantly, and causes the ecotope in aquaculture water to be destroyed, and the general anoxic of water bottom, causes Nitrite accumulation; In some water body, the growth balance of ammonia oxidizing bacteria and nitrite-oxidizing bacteria is broken, and also causes nitrite concentration high, and the lighter affects the fishes and shrimps speed of growth, and severe one causes fish and shrimp poisoning dead.Reducing water nitrite state nitrogen effective ways is to utilize beneficial microorganism effect, and in microbiological treatment approach the most thoroughly, utilize exactly nitrite-oxidizing bacteria to make nitrite nitrogen for nitric nitrogen.Yet due to features such as nitrite-oxidizing bacteria has from nourishing one's nature equally, aerobic, dependency, generation time is long, poor growth, natural quantity in water body is restricted, and causes nitrification not good enough, in the process of purifying water body, can not play desirable effect.Therefore, our screening and separating from sewage obtains strain nitrite-oxidizing bacteria------acinetobacter calcoaceticus, and growth velocity is high, utilize nitrite ability strong, and studied its culture condition.With genus bacillus and yeast compatibility, make complex microorganism preparations, application shows simultaneously, said preparation can obviously be improved brackish water aquaculture water body environment, especially in the aquatic animal summit of growth phase, obviously reduce animal disease, obviously improve cultivated animals quality.
By retrieval, find following 2 pieces of patent documents relevant to present patent application: 1, the invention provides a kind of microbial preparation (CN103663728A) for the improvement of freshwater aquiculture water body, described component comprises: subtilis 25-30wt%; Bacillus licheniformis 25-30wt%; Bacillus megaterium 15-20wt%.2, the invention provides a kind of microbial preparation (CN103667138A) for marine culture water improvement, described component comprises:: subtilis 25-30wt%; Bacillus licheniformis 25-30wt%; Bacillus cereus 15-20wt%.By comparison, the technical scheme of above-mentioned patent document and extraction object all have the different of essence from present patent application, and present patent application has novelty.
Summary of the invention
The object of the invention is to overcome the deficiencies in the prior art part, microbial preparation and preparation method and application for a kind of brackish water aquaculture are provided, the component kind of the method is more, multiple functional, especially acinetobacter calcoaceticus has the ability of efficiently utilizing nitrite, and growth is rapidly, overcome existing microbial preparation nitrobacteria and lacked and poky drawback, for meeting the widespread use of brackish water aquaculture industry, provide possibility.
The present invention solves its technical problem and takes following technical scheme to realize:
A brackish water aquaculture microbial preparation, comprises the following component forming by ratio of weight and the number of copies:
And described carrier is zeolite powder.
A preparation method for microbial preparation for brackish water aquaculture, comprises that method steps is as follows:
The first step, the production production of hybrid seeds;
Second step, by the yeast in the first step, acinetobacter calcoaceticus respectively single culture obtain bacterium liquid, subtilis, bacillus megaterium be the compound bacterium liquid of cultivating to obtain together;
The 3rd step, by each bacterium liquid obtaining in second step subtilis 20-25 part by weight; Bacillus megaterium 10-20 part; Yeast 20-25 part; Acinetobacter calcoaceticus 30-35 part is mixed, and then mixes, is dried, pulverizes, sieves with 100-200 zeolite powder, obtains required microbial preparation.
And in described second step, described subtilis and bacillus megaterium nutrient media components are: glucose 20g, peptone 15g, sodium-chlor 2-10g, extractum carnis 0.5g, distilled water 1L, pH7.0; Described microzyme culture medium component is: potato 200g, glucose 20g, sodium-chlor 2-10g, distilled water 1L, pH7.0; Described acinetobacter calcoaceticus nutrient media components is: sodium acetate 0.5g, SODIUMNITRATE 0.36g, Sodium phosphate dibasic 0.5g, potassium primary phosphate 0.5g, magnesium sulfate 0.1g, sodium-chlor 2-10g, micro-5ml, pH7.2.
And, the cultivation in described second step, its temperature is 25-35 ℃, and optimum condition is 28-32 ℃, and its incubation time is 20-36h, and optimum condition is 24-30h.
An application for microbial preparation for brackish water aquaculture, this microbial preparation is applied to salinity range in the aquaculture water of 2-10 ‰.
Advantage of the present invention and positively effect are:
(1) the present invention solves severe brackish water animal cultivation breeding environment problem by microbial technique, specifically utilize efficient microorganism and meta-bolites thereof, beneficial bacteria in the active bacteria formulation of making through special processing process enters after aquaculture water, can breed rapidly and become dominant bacteria.Beneficial bacteria can be decomposed into rapidly carbonic acid gas, nitrate, phosphoric acid salt, vitriol etc. by organism such as the movement of cultivated animals, residual bait, biological residual bodies, promote unicellular algae Growth and Reproduction, for cultivation object provides oxygen, follow this reciprocal, form an optimum ecological circulation, made the bacterium phase of cultivated animals reach mutually balance with algae, built good water quality environment, provide safety, the Green Product of non-stimulated, pollution-free, noresidue.
(2) microbial preparation of the present invention, described product also has the compound population of beneficial microorganism of enormous amount, can reduce BOD, the COD index of aquaculture water lower curtate, thereby can effectively reduce pond bottom biology and chemical oxygen demand, for cultivated animals provides the bottom ecotope of high-quality, improve the survival rate of cultivated animals, reduce the incidence of disease.
Accompanying drawing explanation
Fig. 1 is that the present invention is that in 3 ‰ water bodys, microbial preparation reduces nitrite nitrogen effect in salinity;
Fig. 2 is that the present invention is that in 10 ‰ water bodys, microbial preparation reduces nitrite nitrogen effect in salinity.
Embodiment
Below in conjunction with accompanying drawing, the invention process is further described, following examples are descriptive, are not determinate, can not limit protection scope of the present invention with this.
A brackish water aquaculture microbial preparation, comprises the following component forming by ratio of weight and the number of copies:
Wherein, described carrier is zeolite powder.
Microbial preparation and a preparation method for brackish water aquaculture, method steps is as follows:
The first step, the production production of hybrid seeds;
Second step, by the yeast in step 1, acinetobacter calcoaceticus respectively single culture obtain bacterium liquid, subtilis, bacillus megaterium be the compound bacterium liquid of cultivating to obtain together;
Wherein, described subtilis and bacillus megaterium nutrient media components are: glucose 20g, peptone 15g, sodium-chlor 2-10g, extractum carnis 0.5g, distilled water 1L, pH7.0.
Wherein, described microzyme culture medium component is: potato 200g (preparation potato is soaked juice), glucose 20g, sodium-chlor 2-10g, distilled water 1L, pH7.0.
Wherein, described acinetobacter calcoaceticus nutrient media components is: sodium acetate 0.5g, SODIUMNITRATE 0.36g, Sodium phosphate dibasic 0.5g, potassium primary phosphate 0.5g, magnesium sulfate 0.1g, sodium-chlor 2-10g, micro-5ml, pH7.2.
The 3rd step, by each bacterium liquid obtaining in second step subtilis 20-25 part by weight; Bacillus megaterium 10-20 part; Yeast 20-25 part; Acinetobacter calcoaceticus 30-35 part is mixed, and then mixes, is dried, pulverizes, sieves with 100-200 part carrier, obtains required microbial preparation;
Wherein, described carrier is zeolite powder;
Wherein, the culture temperature in described second step is preferably 25-35 ℃, 28-32 ℃ more preferably, and incubation time is 20-36h, more preferably 24-30h.
Microorganism system for the improvement of brackish water aquaculture water body of the present invention, viable count >=3,000,000,000/g.
An application for microbial preparation for brackish water aquaculture, this microbial preparation is applied to salinity range in the aquaculture water of 2-10 ‰.
Embodiment 1
Microbial preparation and preparation method thereof for a kind of brackish water aquaculture, comprises the following steps:
The first step, the production production of hybrid seeds;
Second step, the yeast that the first step production of hybrid seeds is obtained, acinetobacter calcoaceticus respectively single culture obtain bacterium liquid, and subtilis, bacillus megaterium be the compound bacterium liquid of cultivating to obtain together, cultivates 25 ℃, incubation time 36h;
The 3rd step, gets subtilis 20g, bacillus megaterium powder 20g, yeast 25g and acinetobacter calcoaceticus 30g and zeolite powder 100g by bacterium liquid weight fraction another name, and mixture is dried, pulverizes, is sieved, and makes microbial preparation.
The application of microbial preparation for a kind of brackish water aquaculture, the microbial preparation that aforesaid method is made is for culture of Penaeus vannamei, the salinity of its aquaculture water is 3 ‰, contrast culture experiment to 10 mu of waters surface, as shown in Figure 1, shrimp disease incidence reduces by 13.5%, and relative growth rate improves 12.9%, obviously reduces cultivation water nitrite content.
Embodiment 2
Microbial preparation and preparation method thereof for a kind of brackish water aquaculture, comprises the following steps:
The first step, the production production of hybrid seeds;
Second step, the yeast that the first step production of hybrid seeds is obtained, acinetobacter calcoaceticus respectively single culture obtain bacterium liquid, and subtilis, bacillus megaterium be the compound bacterium liquid of cultivating to obtain together, cultivates 35 ℃, incubation time 20h;
The 3rd step, gets subtilis 25g, bacillus megaterium powder 10g, yeast 20g and acinetobacter calcoaceticus 35g and zeolite powder 200g by bacterium liquid weight fraction another name, and mixture is dried, pulverizes, is sieved, and makes microbial preparation.
The application of microbial preparation for a kind of brackish water aquaculture, the microbial preparation that aforesaid method is made is for culture of Penaeus vannamei, the salinity of its aquaculture water is 10 ‰, contrast culture experiment to 10 mu of waters surface, as shown in Figure 2, shrimp disease incidence reduces by 15.6%, and relative growth rate improves 11.8%, obviously reduces cultivation water nitrite content.

Claims (6)

1. a brackish water aquaculture microbial preparation, is characterized in that comprising the following component forming by ratio of weight and the number of copies:
2. brackish water aquaculture microbial preparation according to claim 1, is characterized in that: described carrier is zeolite powder.
3. the preparation method of microbial preparation for brackish water aquaculture, is characterized in that comprising that method steps is as follows:
The first step, the production production of hybrid seeds;
Second step, by the yeast in the first step, acinetobacter calcoaceticus respectively single culture obtain bacterium liquid, subtilis, bacillus megaterium be the compound bacterium liquid of cultivating to obtain together;
The 3rd step, by each bacterium liquid obtaining in second step subtilis 20-25 part by weight; Bacillus megaterium 10-20 part; Yeast 20-25 part; Acinetobacter calcoaceticus 30-35 part is mixed, and then mixes, is dried, pulverizes, sieves with 100-200 zeolite powder, obtains required microbial preparation.
4. the preparation method of microbial preparation for brackish water aquaculture according to claim 3, it is characterized in that: in described second step, described subtilis and bacillus megaterium nutrient media components are: glucose 20g, peptone 15g, sodium-chlor 2-10g, extractum carnis 0.5g, distilled water 1L, pH7.0; Described microzyme culture medium component is: potato 200g, glucose 20g, sodium-chlor 2-10g, distilled water 1L, pH7.0; Described acinetobacter calcoaceticus nutrient media components is: sodium acetate 0.5g, SODIUMNITRATE 0.36g, Sodium phosphate dibasic 0.5g, potassium primary phosphate 0.5g, magnesium sulfate 0.1g, sodium-chlor 2-10g, micro-5ml, pH7.2.
5. according to the brackish water aquaculture described in claim 3 or 4, use the preparation method of microbial preparation, it is characterized in that: the cultivation in described second step, its temperature is 25-35 ℃, and optimum condition is 28-32 ℃, and its incubation time is 20-36h, and optimum condition is 24-30h.
6. an application for microbial preparation for brackish water aquaculture, is characterized in that: this microbial preparation is applied to salinity range in the aquaculture water of 2-10 ‰.
CN201410286081.6A 2014-06-24 2014-06-24 Microbial preparation for brackish water aquaculture, and preparation method and application thereof Pending CN104030459A (en)

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Cited By (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN104230004A (en) * 2014-10-16 2014-12-24 内蒙古阜丰生物科技有限公司 Biological agent for processing glutamic acid fermentation waste water
CN105002117A (en) * 2015-08-10 2015-10-28 天津科技大学 Microecological preparation special for silver pomfret and preparing method and application
CN105886448A (en) * 2016-07-06 2016-08-24 苏州市相城区阳澄湖镇东枪塘水产养殖专业合作社 Acid-resistant salt-resistant microbial preparation for aquaculture

Citations (10)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPS62234005A (en) * 1986-04-02 1987-10-14 Seikaken:Kk Microbial preparation for agriculture, forestry and fishery
CN1162016A (en) * 1996-11-07 1997-10-15 中国科学院水生生物研究所 Bacterial strain for producing fishing microbial preparation and cultivating method thereof
CN1861199A (en) * 2005-05-12 2006-11-15 中国农业大学 Biological deodorization and purification agent, and its use
CN101538538A (en) * 2008-03-18 2009-09-23 上海环伟生物科技有限公司 Composite microorganism viable bacteria preparation and preparation method and application of same
CN101921710A (en) * 2010-08-24 2010-12-22 河北省环境科学研究院 Repairing agent for microbes in water bodies of excessive culture zones
CN102146347A (en) * 2009-12-18 2011-08-10 浙江天科高新技术发展有限公司 Acinetobacter sp. and application of composite bacterial agent of acinetobacter species
CN102212476A (en) * 2011-03-07 2011-10-12 杭州向天歌生物科技有限公司 Method for cleanly and efficiently producing microorganism bactericide
CN102220262A (en) * 2011-04-29 2011-10-19 哈尔滨恩瑞科技发展有限公司 Composite microbiological preparation and preparation method and use thereof
CN102719380A (en) * 2012-06-15 2012-10-10 天津昌农科技有限责任公司 Aquatic compound microecologics and preparation method thereof
CN103013851A (en) * 2012-10-08 2013-04-03 上海交通大学 Complex microbial inoculant and applications thereof

Patent Citations (10)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPS62234005A (en) * 1986-04-02 1987-10-14 Seikaken:Kk Microbial preparation for agriculture, forestry and fishery
CN1162016A (en) * 1996-11-07 1997-10-15 中国科学院水生生物研究所 Bacterial strain for producing fishing microbial preparation and cultivating method thereof
CN1861199A (en) * 2005-05-12 2006-11-15 中国农业大学 Biological deodorization and purification agent, and its use
CN101538538A (en) * 2008-03-18 2009-09-23 上海环伟生物科技有限公司 Composite microorganism viable bacteria preparation and preparation method and application of same
CN102146347A (en) * 2009-12-18 2011-08-10 浙江天科高新技术发展有限公司 Acinetobacter sp. and application of composite bacterial agent of acinetobacter species
CN101921710A (en) * 2010-08-24 2010-12-22 河北省环境科学研究院 Repairing agent for microbes in water bodies of excessive culture zones
CN102212476A (en) * 2011-03-07 2011-10-12 杭州向天歌生物科技有限公司 Method for cleanly and efficiently producing microorganism bactericide
CN102220262A (en) * 2011-04-29 2011-10-19 哈尔滨恩瑞科技发展有限公司 Composite microbiological preparation and preparation method and use thereof
CN102719380A (en) * 2012-06-15 2012-10-10 天津昌农科技有限责任公司 Aquatic compound microecologics and preparation method thereof
CN103013851A (en) * 2012-10-08 2013-04-03 上海交通大学 Complex microbial inoculant and applications thereof

Non-Patent Citations (1)

* Cited by examiner, † Cited by third party
Title
袁春营等: "益菌调水宝在南美白对虾养殖中的应用", 《水生态学杂志》 *

Cited By (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN104230004A (en) * 2014-10-16 2014-12-24 内蒙古阜丰生物科技有限公司 Biological agent for processing glutamic acid fermentation waste water
CN104230004B (en) * 2014-10-16 2015-10-14 内蒙古阜丰生物科技有限公司 A kind of biotechnological formulation processing glutamic acid fermentation waste water
CN105002117A (en) * 2015-08-10 2015-10-28 天津科技大学 Microecological preparation special for silver pomfret and preparing method and application
CN105886448A (en) * 2016-07-06 2016-08-24 苏州市相城区阳澄湖镇东枪塘水产养殖专业合作社 Acid-resistant salt-resistant microbial preparation for aquaculture

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Application publication date: 20140910