CN103535301B - Method for cultivating sea cucumber larvae - Google Patents
Method for cultivating sea cucumber larvae Download PDFInfo
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- CN103535301B CN103535301B CN201310504671.7A CN201310504671A CN103535301B CN 103535301 B CN103535301 B CN 103535301B CN 201310504671 A CN201310504671 A CN 201310504671A CN 103535301 B CN103535301 B CN 103535301B
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
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- Y02A40/00—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
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Abstract
The invention discloses a method for cultivating sea cucumber larvae. The method includes steps of distributing sea cucumber fries and cultivating the larvae; feeding mixed liquid fodder containing simulated fine nannochloris oculata dry powder, yeast and EM (effective microorganisms) to the larvae after larva embryos are developed into the small auricularia larvae and the digestive tracts of the small auricularia larvae are completely developed; enabling the auricularia larvae to start to be gradually developed into doliolarias at the end of the fifth feeding day; completing sea cucumber larva cultivation after the eighth feeding day; starting a juvenile sea cucumber cultivating stage. Compared with the traditional method for cultivating sea cucumber larvae, the method for cultivating the sea cucumber larvae has the advantages that the method is simple and convenient, fodder cultivating workshops can be omitted, labor and materials can be saved, the production cost can be reduced, and the fodder can be stably supplied, is safe and is comprehensively nutritional.
Description
Technical field
The present invention relates to the cultural method of a kind of sea cucumber, particularly relate to a kind of cultural method for sea cucumber auricularia.
Background technology
Traditional bait of breading sea cucumber seedlings is Chaetoceros, salt algae, micro-algae such as Nitzschia closterlum, but the production of bait micro-algae takies production equipment, material resources, manpower, cost is higher, also has certain risk, especially in cloudy weather for several days running sky, Mirco algal food is not easy growth, once bait supply is not gone up, causes heavy losses to nursery.The most bait using dried yeast as sea cucumber auricularia of existing seedling growing process.And dried yeast more micro-algae in nutrition has a certain distance, and some in the past report utilize Nannochloropsis oculata to cultivate the experiment of sea cucumber larva, all do not obtain desirable experiment effect.
Summary of the invention
The technical problem to be solved in the present invention is to provide a kind of cultural method utilizing plan Nannochloropsis oculata dry powder to combine the sea cucumber young of throwing something and feeding with yeast.
A cultural method for the sea cucumber young, comprises the steps:
First sea cucumber cloth seedling and larvae cultivation is carried out, when described larva embryonic development is to little auricularia, after gut development is complete, start to throw something and feed and intend Nannochloropsis oculata dry powder, yeast, the mixed liquor feed of EM bacterium, the 5th day end of throwing something and feeding starts to gradually become doliolaria, the 8th day sea cucumber young of throwing something and feeding is cultivated and terminates, and enters the cultivation stage of young ginseng.
The cultural method of the sea cucumber young of the present invention, wherein:
First day feeding volume is: the yeast concentration cultivated in pond is 0.3ppm, and plan Nannochloropsis oculata dry powder concentration is 0.2ppm, EM bacteria concentration is 5ml/m
3;
From the 2nd day, day feeding volume be adjusted to: to cultivate in pond yeast and intend Nannochloropsis oculata dry powder 0.4 ~ 0.8ppm, EM bacterium 2 ~ 5ml/m
3, wherein, described yeast is 1:1 with the mass ratio of plan Nannochloropsis oculata dry powder.
The cultural method of the sea cucumber young of the present invention, wherein, the stomach situation of the young described in microscopy before throwing something and feeding, when stomach be less than 1/4 body of stomach amass time, feeding volume the previous day basis increases 0.1-0.2g/m
3mixed liquor feed, stomach is greater than 1/3 body of stomach when amassing, and 0.1-0.2g/m is reduced on feeding volume the previous day basis
3mixed liquor feed; From to throw something and feed beginning, middle ear juvenile stage is entered to the 3rd day described young, within 4th day, enter the large auricularia stage, 5 pairs of orbicules go out entirely gradually, the former base of five tentacles former base radial canal starts differentiation, and within the 5th day, end starts to gradually become doliolaria, when the 6th day microscopy larva has a 10%-30% doliolarias, change water 50% with 200 mesh sieve tulle casees, throw in adherance; Within 8th day, sea cucumber larva terminates life of swimming, and attachment, metamorphosis is young ginseng, changes young ginseng open-mouthed bait of throwing something and feeding, enters the cultivation stage of young ginseng, and sea cucumber young cultivation stage terminates.
The cultural method of the sea cucumber young of the present invention, wherein throw something and feed in process described, 1-5 days every days threw something and fed once, before 2-5 days throw something and feed the young described in microscopy stomach situation and adjust feeding volume, the feeding volume of the 6th day and the 7th day was with the feeding volume of the 5th day, throw something and feed every day twice, two minor tick 12h, the half of day feeding volume of at every turn throwing something and feeding.
The cultural method of the sea cucumber young of the present invention, wherein said EM bacterium comprises bacillus group and lactobacillus, and viable count is 10,000,000,000/ml.
The cultural method of the sea cucumber young of the present invention, wherein, before throwing something and feeding, by described yeast cultivation clean sea water immersion 10 minutes, then reinstates the above bolting silk net filtration of 200 order with described plan Nannochloropsis oculata dry powder one, then adds EM bacterium and mix.
The cultural method of the sea cucumber young of the present invention, wherein said plan Nannochloropsis oculata dry powder adopts fresh plan Nannochloropsis oculata to obtain after spraying dry and mechanical breaking-wall method process successively.
The cultural method of the sea cucumber young of the present invention, wherein said larvae cultivation water temperature is 20 ~ 27 DEG C, initial stage cultivation density is less than 1/ml and is greater than 3/ml, to the seawater adding 1/3 cultivation pond volume during larva cloth seedling, from first day is thrown something and fed, every day adds the fresh seawater of cultivating pond volume described in 1/6, to filling it up with described cultivation pond.
The cultural method of the sea cucumber young of the present invention, wherein said larvae cultivation water temperature is 22 ~ 25 DEG C.
The cultural method difference from prior art of the sea cucumber young of the present invention is:
The cultural method of the sea cucumber young of the present invention is than classical culture protocols, easy, save bait and cultivate workshop, manpower, material resources, reduce production cost, and bait supply is stablized, safety, comprehensive nutrition; Synthermal, same culture density, and under the condition of management method, use this cultural method, sea cucumber larvae development carries the previous day to the large ear post-larval time, the stomach shape of larva is full, and coat of the stomach is thin, and orbicule color is dark, five tentacles and the former base differentiation of radial canal are obviously, to cup shape, pentactula, and the distortion ratio in young ginseng each stage is high.
In cultural method of the present invention, the yeast of use is marine yeast dry powder, and EM bacterium flora comprises and keeps active bacillus flora and lactic acid bacteria flora; Intend Nannochloropsis oculata dry powder be fresh plan Nannochloropsis oculata after super-dry again through broken wall treatment, not only remain nutrition contained by it but also be easier to digest and assimilate; Add saccharomycete, lactobacillus, after the mixed liquor feed of bacillus flora, the sea cucumber young has the ability saccharomycete to digest completely, add EM bacterium, make various ferment in sea cucumber larva gastric juice reach a higher level, thus plan Nannochloropsis oculata dry powder is digested smoothly, thus comprehensively nutrient component is absorbed; From bait throwing in, along with larval growth, add the new water cultivating pond volume 1/6 amount every day, reach to evacuate larval density and improve and cultivate water environment object.Feeding volume makes to form a certain amount of food density in cultivation pond first, make auricularia can be easy to feed into various bait particle, along with in the unit of water body of ingesting of auricularia in cultivation water body, bait particle reduces, the bait that auricularia feeds within the unit interval just reduces, also the many of stomach or few will be shown when microscopy, the object getting hold of daily ration, feeding quantity within the scope of this agreement is, auricularia should be met within unit interval and space, have enough bait particle filter foods, meet growing of larva, that avoids bait in water body too much to cause water quality again ruins pollution problem.
Embodiment
Embodiment 1
A cultural method for the sea cucumber young, comprises the steps:
First sea cucumber cloth seedling and larvae cultivation is carried out: described larvae cultivation water temperature is 20 ~ 27 DEG C, preferably 22 ~ 25 DEG C, initial stage cultivation density is less than 1/ml and is greater than 3/ml, to the seawater adding 1/3 cultivation pond volume during larva cloth seedling, from first day is thrown something and fed, every day adds the fresh seawater of cultivating pond volume described in 1/6, to filling it up with described cultivation pond.
When described larva embryonic development is to little auricularia, after gut development is complete, starts to throw something and feed and intend Nannochloropsis oculata dry powder, yeast, the mixed liquor feed of EM bacterium, the 5th day end of throwing something and feeding starts to gradually become doliolaria, the 8th day sea cucumber young of throwing something and feeding is cultivated and terminates, and enters the cultivation stage of young ginseng.
First day feeding volume is: the yeast concentration cultivated in pond is 0.3ppm, and plan Nannochloropsis oculata dry powder concentration is 0.2ppm, EM bacteria concentration is 5ml/m
3; From the 2nd day, day feeding volume be adjusted to: to cultivate in pond yeast and intend Nannochloropsis oculata dry powder 0.4 ~ 0.8ppm, EM bacterium 2 ~ 5ml/m
3, wherein, described yeast is 1:1 with the mass ratio of plan Nannochloropsis oculata dry powder.The stomach situation of the young described in microscopy before throwing something and feeding, when stomach be less than 1/4 body of stomach amass time, feeding volume the previous day basis increases 0.1-0.2g/m
3mixed liquor feed, stomach is greater than 1/3 body of stomach when amassing, and 0.1-0.2g/m is reduced on feeding volume the previous day basis
3mixed liquor feed; From to throw something and feed beginning, middle ear juvenile stage is entered to the 3rd day described young, within 4th day, enter the large auricularia stage, 5 pairs of orbicules go out entirely gradually, the former base of five tentacles former base radial canal starts differentiation, and within the 5th day, end starts to gradually become doliolaria, when the 6th day microscopy larva has a 10%-30% doliolarias, change water 50% with 200 mesh sieve tulle casees, throw in adherance; Within 8th day, sea cucumber larva terminates life of swimming, and attachment, metamorphosis is young ginseng, changes young ginseng open-mouthed bait of throwing something and feeding, enters the cultivation stage of young ginseng, and sea cucumber young cultivation stage terminates.
Throw something and feed in process described, 1-5 days every days threw something and fed once, before 2-5 days throw something and feed the young described in microscopy stomach situation and according to said method adjustment feeding volume, the feeding volume of the 6th day and the 7th day was with the feeding volume of the 5th day, throw something and feed every day twice, two minor tick 12h, the half of day feeding volume of at every turn throwing something and feeding.Described EM bacterium comprises bacillus group and lactobacillus, and viable count is 10,000,000,000/ml; Before throwing something and feeding, by described yeast cultivation clean sea water immersion 10 minutes, then reinstate the above bolting silk net filtration of 200 order with described plan Nannochloropsis oculata dry powder one, then add EM bacterium and mix; Described plan Nannochloropsis oculata dry powder adopts fresh plan Nannochloropsis oculata to obtain after spraying dry and mechanical breaking-wall method process successively.
Above-described embodiment is only be described the preferred embodiment of the present invention; not scope of the present invention is limited; under not departing from the present invention and designing the prerequisite of spirit; the various distortion that those of ordinary skill in the art make technical scheme of the present invention and improvement, all should fall in protection domain that claims of the present invention determines.
Claims (5)
1. a cultural method for the sea cucumber young, is characterized in that: comprise the steps:
First sea cucumber cloth seedling and larvae cultivation is carried out, when described larva embryonic development is to little auricularia, after gut development is complete, start to throw something and feed and intend Nannochloropsis oculata dry powder, yeast, the mixed liquor feed of EM bacterium, the 5th day end of throwing something and feeding starts to gradually become doliolaria, the 8th day sea cucumber young of throwing something and feeding is cultivated and terminates, and enters the cultivation stage of young ginseng;
First day feeding volume is: the yeast concentration cultivated in pond is 0.3ppm, and plan Nannochloropsis oculata dry powder concentration is 0.2ppm, EM bacteria concentration is 5ml/m
3;
From the 2nd day, day feeding volume be adjusted to: to cultivate in pond yeast and intend Nannochloropsis oculata dry powder 0.4 ~ 0.8ppm, EM bacterium 2 ~ 5ml/m
3, wherein, described yeast is 1:1 with the mass ratio of plan Nannochloropsis oculata dry powder;
The stomach situation of the young described in microscopy before throwing something and feeding, when stomach be less than 1/4 body of stomach amass time, feeding volume the previous day basis increases 0.1-0.2g/m
3mixed liquor feed, stomach is greater than 1/3 body of stomach when amassing, and 0.1-0.2g/m is reduced on feeding volume the previous day basis
3mixed liquor feed; From to throw something and feed beginning, middle ear juvenile stage is entered to the 3rd day described young, within 4th day, enter the large auricularia stage, 5 pairs of orbicules go out entirely gradually, the former base of five tentacles former base radial canal starts differentiation, and within the 5th day, end starts to gradually become doliolaria, when the 6th day microscopy larva has a 10%-30% doliolarias, change water 50% with 200 mesh sieve tulle casees, throw in adherance; Within 8th day, sea cucumber larva terminates life of swimming, and attachment, metamorphosis is young ginseng, changes young ginseng open-mouthed bait of throwing something and feeding, enters the cultivation stage of young ginseng, and sea cucumber young cultivation stage terminates.
2. the cultural method of the sea cucumber young according to claim 1, is characterized in that: throw something and feed in process described, 1-5 days every days threw something and fed once; Before 2-5 days throw something and feed the young described in microscopy stomach situation and adjust feeding volume, the feeding volume of the 6th day and the 7th day, with the feeding volume of the 5th day, is thrown something and fed twice every day, two minor tick 12h, the half of day feeding volume of at every turn throwing something and feeding.
3. the cultural method of the sea cucumber young according to claim 1 and 2, is characterized in that: described EM bacterium comprises bacillus group and lactobacillus, and viable count is 10,000,000,000/ml.
4. the cultural method of the sea cucumber young according to claim 1 and 2, it is characterized in that: before throwing something and feeding, by described yeast cultivation clean sea water immersion 10 minutes, then reinstate the above bolting silk net filtration of 200 order with described plan Nannochloropsis oculata dry powder one, then add EM bacterium and mix.
5. the cultural method of the sea cucumber young according to claim 1 and 2, is characterized in that: described plan Nannochloropsis oculata dry powder adopts fresh plan Nannochloropsis oculata to obtain after spraying dry and mechanical breaking-wall method process successively.
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| CN201310504671.7A CN103535301B (en) | 2013-10-23 | 2013-10-23 | Method for cultivating sea cucumber larvae |
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| CN104719206B (en) * | 2015-03-23 | 2017-07-25 | 海南定利养殖种苗有限公司 | A kind of mating system of Holothuria scabra artificial seed |
| CN110476849A (en) * | 2019-09-27 | 2019-11-22 | 烟台市海洋经济研究院 | A kind of water changing method that stichopus japonicus auricularia larvas is cultivated |
| CN114631596A (en) * | 2020-12-15 | 2022-06-17 | 大连鑫玉龙海洋生物种业科技股份有限公司 | Special paste bait for sea cucumber auricular larvae |
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| CN103039722B (en) * | 2012-12-05 | 2014-07-16 | 大连海洋大学 | Active biological composite bait for marine animals and preparation method thereof |
| CN103156092B (en) * | 2013-03-22 | 2014-11-26 | 山东省海洋水产研究所 | Saltwater fish compound feed taking microalgae flour as fat source and processing method thereof |
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Effective date of registration: 20151009 Address after: 265601 Zhenhai Road North gully, Penglai, Shandong Patentee after: Yantai Hai Rong microalgae breeding Co.,Ltd. Address before: 264006 No. 1, No. 28, Yinhe Road, 2, Yantai Development Zone, Shandong Patentee before: YANTAI HAIRONG ELECTRIC POWER TECHNOLOGY Co.,Ltd. |
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Effective date of registration: 20230626 Address after: No. 288, Nanwujia Village, Liujiagou Town, Penglai District, Yantai City, Shandong Province 265601 Patentee after: Yantai Lishang mariculture Co.,Ltd. Address before: 265601 Hairun North Road, Beigou Town, Penglai City, Shandong Province Patentee before: Yantai Hai Rong microalgae breeding Co.,Ltd. |
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