CN110257363A - A kind of preparation method and applications of Immobilized hyphae microballoon - Google Patents
A kind of preparation method and applications of Immobilized hyphae microballoon Download PDFInfo
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- 238000002360 preparation method Methods 0.000 title claims abstract description 15
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims abstract description 47
- 241000195493 Cryptophyta Species 0.000 claims abstract description 38
- 241000894006 Bacteria Species 0.000 claims abstract description 29
- 238000000034 method Methods 0.000 claims abstract description 13
- IXPNQXFRVYWDDI-UHFFFAOYSA-N 1-methyl-2,4-dioxo-1,3-diazinane-5-carboximidamide Chemical compound CN1CC(C(N)=N)C(=O)NC1=O IXPNQXFRVYWDDI-UHFFFAOYSA-N 0.000 claims abstract description 9
- 235000010413 sodium alginate Nutrition 0.000 claims abstract description 9
- 239000000661 sodium alginate Substances 0.000 claims abstract description 9
- 229940005550 sodium alginate Drugs 0.000 claims abstract description 9
- 239000000463 material Substances 0.000 claims abstract description 7
- 241000195649 Chlorella <Chlorellales> Species 0.000 claims abstract description 6
- 239000012153 distilled water Substances 0.000 claims description 13
- 239000010865 sewage Substances 0.000 claims description 13
- OKTJSMMVPCPJKN-UHFFFAOYSA-N Carbon Chemical compound [C] OKTJSMMVPCPJKN-UHFFFAOYSA-N 0.000 claims description 10
- 229910052799 carbon Inorganic materials 0.000 claims description 10
- 239000010802 sludge Substances 0.000 claims description 8
- 239000002028 Biomass Substances 0.000 claims description 6
- 238000012545 processing Methods 0.000 claims description 6
- 238000000227 grinding Methods 0.000 claims description 5
- 238000011010 flushing procedure Methods 0.000 claims description 4
- 239000007788 liquid Substances 0.000 claims description 4
- 238000010521 absorption reaction Methods 0.000 claims description 3
- 238000005119 centrifugation Methods 0.000 claims description 3
- 239000008367 deionised water Substances 0.000 claims description 3
- 229910021641 deionized water Inorganic materials 0.000 claims description 3
- 238000010612 desalination reaction Methods 0.000 claims description 3
- 229910017053 inorganic salt Inorganic materials 0.000 claims description 3
- 238000002156 mixing Methods 0.000 claims description 3
- 239000006228 supernatant Substances 0.000 claims description 3
- 239000001110 calcium chloride Substances 0.000 claims description 2
- 229910001628 calcium chloride Inorganic materials 0.000 claims description 2
- 238000004132 cross linking Methods 0.000 claims description 2
- 238000004090 dissolution Methods 0.000 claims 1
- 238000003756 stirring Methods 0.000 claims 1
- IJGRMHOSHXDMSA-UHFFFAOYSA-N Atomic nitrogen Chemical compound N#N IJGRMHOSHXDMSA-UHFFFAOYSA-N 0.000 abstract description 24
- OAICVXFJPJFONN-UHFFFAOYSA-N Phosphorus Chemical compound [P] OAICVXFJPJFONN-UHFFFAOYSA-N 0.000 abstract description 19
- 229910052698 phosphorus Inorganic materials 0.000 abstract description 18
- 239000011574 phosphorus Substances 0.000 abstract description 18
- 229910052757 nitrogen Inorganic materials 0.000 abstract description 12
- YUWBVKYVJWNVLE-UHFFFAOYSA-N [N].[P] Chemical compound [N].[P] YUWBVKYVJWNVLE-UHFFFAOYSA-N 0.000 abstract description 8
- 235000001674 Agaricus brunnescens Nutrition 0.000 abstract description 4
- 230000015556 catabolic process Effects 0.000 abstract description 2
- 238000006731 degradation reaction Methods 0.000 abstract description 2
- 230000001473 noxious effect Effects 0.000 abstract description 2
- 239000005416 organic matter Substances 0.000 abstract description 2
- 231100000331 toxic Toxicity 0.000 abstract description 2
- 230000002588 toxic effect Effects 0.000 abstract description 2
- 230000002195 synergetic effect Effects 0.000 abstract 1
- XKMRRTOUMJRJIA-UHFFFAOYSA-N ammonia nh3 Chemical compound N.N XKMRRTOUMJRJIA-UHFFFAOYSA-N 0.000 description 8
- 230000000694 effects Effects 0.000 description 7
- QGZKDVFQNNGYKY-UHFFFAOYSA-N Ammonia Chemical compound N QGZKDVFQNNGYKY-UHFFFAOYSA-N 0.000 description 4
- QVGXLLKOCUKJST-UHFFFAOYSA-N atomic oxygen Chemical compound [O] QVGXLLKOCUKJST-UHFFFAOYSA-N 0.000 description 3
- 230000006872 improvement Effects 0.000 description 3
- 229910052760 oxygen Inorganic materials 0.000 description 3
- 239000001301 oxygen Substances 0.000 description 3
- 230000029553 photosynthesis Effects 0.000 description 3
- 238000010672 photosynthesis Methods 0.000 description 3
- 230000008569 process Effects 0.000 description 3
- 238000005070 sampling Methods 0.000 description 3
- 239000000126 substance Substances 0.000 description 3
- CURLTUGMZLYLDI-UHFFFAOYSA-N Carbon dioxide Chemical compound O=C=O CURLTUGMZLYLDI-UHFFFAOYSA-N 0.000 description 2
- VVQNEPGJFQJSBK-UHFFFAOYSA-N Methyl methacrylate Chemical compound COC(=O)C(C)=C VVQNEPGJFQJSBK-UHFFFAOYSA-N 0.000 description 2
- 229920005372 Plexiglas® Polymers 0.000 description 2
- 229910021529 ammonia Inorganic materials 0.000 description 2
- 239000003795 chemical substances by application Substances 0.000 description 2
- 208000028659 discharge Diseases 0.000 description 2
- 238000011049 filling Methods 0.000 description 2
- 238000005259 measurement Methods 0.000 description 2
- 230000004060 metabolic process Effects 0.000 description 2
- 244000005700 microbiome Species 0.000 description 2
- 239000004005 microsphere Substances 0.000 description 2
- 238000012986 modification Methods 0.000 description 2
- 230000004048 modification Effects 0.000 description 2
- 238000000746 purification Methods 0.000 description 2
- 238000011160 research Methods 0.000 description 2
- UXVMQQNJUSDDNG-UHFFFAOYSA-L Calcium chloride Chemical compound [Cl-].[Cl-].[Ca+2] UXVMQQNJUSDDNG-UHFFFAOYSA-L 0.000 description 1
- 240000009108 Chlorella vulgaris Species 0.000 description 1
- 235000007089 Chlorella vulgaris Nutrition 0.000 description 1
- 241000196324 Embryophyta Species 0.000 description 1
- 206010021143 Hypoxia Diseases 0.000 description 1
- 240000002853 Nelumbo nucifera Species 0.000 description 1
- 235000006508 Nelumbo nucifera Nutrition 0.000 description 1
- 235000006510 Nelumbo pentapetala Nutrition 0.000 description 1
- 238000005273 aeration Methods 0.000 description 1
- 239000011324 bead Substances 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 229910002092 carbon dioxide Inorganic materials 0.000 description 1
- 239000001569 carbon dioxide Substances 0.000 description 1
- 230000008859 change Effects 0.000 description 1
- 239000000356 contaminant Substances 0.000 description 1
- 230000007812 deficiency Effects 0.000 description 1
- 238000005516 engineering process Methods 0.000 description 1
- 230000007613 environmental effect Effects 0.000 description 1
- 239000003344 environmental pollutant Substances 0.000 description 1
- 238000002474 experimental method Methods 0.000 description 1
- 239000000945 filler Substances 0.000 description 1
- 239000008187 granular material Substances 0.000 description 1
- 239000001963 growth medium Substances 0.000 description 1
- 238000003306 harvesting Methods 0.000 description 1
- 230000007954 hypoxia Effects 0.000 description 1
- 238000005286 illumination Methods 0.000 description 1
- 239000010842 industrial wastewater Substances 0.000 description 1
- 239000003446 ligand Substances 0.000 description 1
- 239000011159 matrix material Substances 0.000 description 1
- 239000000203 mixture Substances 0.000 description 1
- 231100000252 nontoxic Toxicity 0.000 description 1
- 230000003000 nontoxic effect Effects 0.000 description 1
- 235000015097 nutrients Nutrition 0.000 description 1
- 239000002245 particle Substances 0.000 description 1
- 230000035699 permeability Effects 0.000 description 1
- 239000002957 persistent organic pollutant Substances 0.000 description 1
- 230000001766 physiological effect Effects 0.000 description 1
- 231100000719 pollutant Toxicity 0.000 description 1
- 239000007787 solid Substances 0.000 description 1
- 239000013589 supplement Substances 0.000 description 1
- 230000031068 symbiosis, encompassing mutualism through parasitism Effects 0.000 description 1
- 238000012549 training Methods 0.000 description 1
- 239000002351 wastewater Substances 0.000 description 1
Classifications
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- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F3/00—Biological treatment of water, waste water, or sewage
- C02F3/32—Biological treatment of water, waste water, or sewage characterised by the animals or plants used, e.g. algae
- C02F3/322—Biological treatment of water, waste water, or sewage characterised by the animals or plants used, e.g. algae use of algae
- C02F3/325—Biological treatment of water, waste water, or sewage characterised by the animals or plants used, e.g. algae use of algae as symbiotic combination of algae and bacteria
-
- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F3/00—Biological treatment of water, waste water, or sewage
- C02F3/34—Biological treatment of water, waste water, or sewage characterised by the microorganisms used
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N11/00—Carrier-bound or immobilised enzymes; Carrier-bound or immobilised microbial cells; Preparation thereof
- C12N11/02—Enzymes or microbial cells immobilised on or in an organic carrier
- C12N11/04—Enzymes or microbial cells immobilised on or in an organic carrier entrapped within the carrier, e.g. gel or hollow fibres
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N11/00—Carrier-bound or immobilised enzymes; Carrier-bound or immobilised microbial cells; Preparation thereof
- C12N11/02—Enzymes or microbial cells immobilised on or in an organic carrier
- C12N11/10—Enzymes or microbial cells immobilised on or in an organic carrier the carrier being a carbohydrate
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- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F2101/00—Nature of the contaminant
- C02F2101/10—Inorganic compounds
- C02F2101/16—Nitrogen compounds, e.g. ammonia
-
- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F2101/00—Nature of the contaminant
- C02F2101/30—Organic compounds
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- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F2209/00—Controlling or monitoring parameters in water treatment
- C02F2209/14—NH3-N
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Abstract
The invention discloses a kind of preparation method and applications of Immobilized hyphae microballoon, using the method that PVA and sodium alginate complex carrier embed come Immobilized hyphae, with the synergistic effect of the algae solution and bacterium solution embedded in Immobilized hyphae microballoon, so that nitrogen phosphorus and organic matter degradation in water body, avoid toxic action of the extraneous water body noxious material to mushroom and algae in handling black smelly water.Immobilized hyphae microballoon has preferable Nitrogen/Phosphorus Removal to black smelly water compared to relatively free chlorella and bacterium.
Description
Technical field
The present invention relates to a kind of preparation method and applications of Immobilized hyphae microballoon, belong to sewage purification field.
Background technique
Urban river not only supplies water, while also having become the primary discharge place of urban industry waste water, resident living sewage.
With a large amount of discharges of industrial wastewater and sanitary sewage, organic carbon pollutant, organic based nitrogen contaminant in river and contain phosphatization
Object load is closed to continue to increase.So that degradable organic pollutant consumes a large amount of dissolved oxygen of water body, water hypoxia, water quality is caused to dislike
Change, black it is smelly.
Immobilized hyphae microballoon processing sewage is high with biological density, treatment effeciency is high, frustule is easy to harvest and resistance to negative
The advantages that lotus is an important research direction of the environmental area in recent years in process for removing nitrogen and phosphor from sewage research.Practical water body
Algae is absorbed the nutriments such as nitrogen, the phosphorus in sewage by photosynthesis and used for itself in purification, and mushroom microorganism utilizes
The O that photosynthesis of plant generates2, degradation of organic substances is purified sewage also by the symbiosis between bacterium algae.It is existing
Technology there are the shortcomings that include that immobilized microspheres higher cost, single carrier are fixed be not easy to that forming, immobilization time is long, anti-impact
Ability difference etc. is hit, the requirement for bacterium algae microballoon also needs the matrix of bead good etc. with permeability, physics and chemical stability
Characteristic, and it is nontoxic to microorganism in immobilization process and after immobilization.
Summary of the invention
The present invention provides a kind of preparation of Immobilized hyphae microballoon to overcome above-mentioned the deficiencies in the prior art
Method and its application.The method of the present invention is easy to operate, the single easy acquisition of agents useful for same, practical using wide, to the processing of nitrogen phosphorus
Effect is good.
The preparation method of Immobilized hyphae microballoon of the present invention, includes the following steps:
Step 1: taking culture to the chlorella algae solution (chlorella algae is commercially available acquisition) of logarithmic phase, in 3500r/min item
It is centrifuged 10min under part, wash with distilled water, is centrifuged to remove the inorganic salt and other material being attached on frustule, is saved at 5 DEG C
It is spare；
Step 2: taking the activated sludge of certain volume, be placed in centrifuge tube and stand 1h, after centrifugation (3500r/min, 10min)
Liquid is discarded supernatant, with 1min is centrifuged after distilled water again, is stored for future use at 5 DEG C after concentration；
Step 3: distilled water flushing active carbon is used, baking oven is then placed in, it is spare after grinding in 50 DEG C~60 DEG C dry 12h；
Step 4: a certain amount of PVA and sodium alginate being put into beaker, suitable distilled water is added, under 90 DEG C of water-baths
It is completely dissolved, 30 DEG C is naturally cooled to after being completely dissolved, form PVA-SA gel.
Step 5: the algae solution that step 1 obtains and the activated sludge for the concentration that step 2 obtains being mixed, after being diluted with distilled water
Obtain bacterium algae mixed liquor；The active carbon that step 3 obtains is added into gained bacterium algae mixed liquor, then mixing and absorption 10min will be mixed
Conjunction liquid, which is added in PVA-SA gel, to be stirred and evenly mixed, and is added drop-wise to CaCl with syringe2In solution, 2h, crosslinking 2h are fixed at room temperature
After filter out microballoon, with deionized water desalination 2h, finally obtain Immobilized hyphae microballoon.
Activated sludge in step 2 is taken from laboratory aeration tank, and main parameter is SV (sludge settling ratio): 21%,
SVI (sludge index): 101mg/L, MLSS (mixture suspended solid): 1500mg/L.
In step 3,60 meshes are crossed after active carbon grinding.
In step 4, the mass concentration of PVA is 10%~12% in PVA-SA gel, and the mass concentration of sodium alginate is
0.2%~0.6%.
In step 5, preferred phycomycete biomass ratio is 2:1 in bacterium algae mixed liquor.
In step 5, the dosage of active carbon accounts for the 0.8%~1.2% of bacterium algae mixed liquor gross mass.
In step 5, the volume ratio of bacterium algae mixed liquor and PVA-SA gel is 2:3.
In step 5, CaCl2The mass concentration of solution is 1.5%~2.5%.
In step 5, the liquid-drop diameter oozed by syringe is controlled in 5~7mm.
The application of Immobilized hyphae microballoon prepared by the present invention is the place that the Immobilized hyphae microballoon is used for sewage
Reason.Specifically Immobilized hyphae microballoon is put into sewage, nitrogen, phosphorus nutrients in algal grown metabolic process in absorption water
Matter, while oxygen is generated using photosynthesis, supplement water body；Organic matter and part nitrogen phosphorus in the growth metabolism consumption water of mushroom
Substance, while the dissolved oxygen in water body is consumed, generate the carbon dioxide utilized for algae.Reach sewage by above procedure
The effect of denitrogenation dephosphorizing.
The optimal distribution density of the Immobilized hyphae microballoon is 5500~6500/dm3, preferably 6000/dm3。
With the optimum temperature of Immobilized hyphae microballoon processing sewage for 25 DEG C~30 DEG C.
Compared with prior art, the beneficial effects of the present invention are embodied in:
1, the present invention provides a kind of methods that Immobilized hyphae microballoon handles black smelly water.It is single for fixation support
Embedded using sodium alginate, be mainly characterized in that balling-up be easy and be not easy bonding but it is also easily biodegradable, density is big；It is single
One embedded using PVA, is mainly characterized in that balling-up is difficult, easily bonding, sinking time is long.So using of optimizing of the present invention is suitable
The PVA and sodium alginate complex carrier of proportion embed bacterium algae, and obtained bacterium algae microballoon high mechanical strength is not easy to reveal, with watertight
It spends close, can suspend.
2, the present invention is handling black smelly water using the method that PVA and sodium alginate complex carrier embed come Immobilized hyphae
In avoid toxic action of the extraneous water body noxious material to mushroom and algae.Immobilized hyphae microballoon compared to relatively free chlorella and
Bacterium has preferable Nitrogen/Phosphorus Removal to black smelly water.
3, the method that the present invention prepares Immobilized hyphae microballoon has easy to operate, the single easy acquisition of agents useful for same, application
Extensively, the advantages that practical, to nitrogen phosphorus high treating effect, has broad application prospects in black and odorous water improvement.
Detailed description of the invention
Fig. 1 is influence of the different phycomycete ratios to removal efficiency of nitrogen and phosphorus in black smelly water.Wherein (a) Immobilized hyphae is to black smelly water
The accumulative removal rate of middle ammonia nitrogen, (b) accumulative removal rate of the Immobilized hyphae to total phosphorus in black smelly water.From figure 1 it appears that root
It is arranged from big to small according to the removal rate of ammonia nitrogen, phycomycete ratio is followed successively by 1:2,2:1,1:1.And according to the removal rate of total phosphorus from greatly to
Minispread, phycomycete ratio are followed successively by 2:1,1:1,1:2.Effect and biomass and the carrier inside for comprehensively considering nitrogen phosphorus ligands are empty
Between between contradiction, phycomycete ratio be 2:1 when, Nitrogen/Phosphorus Removal is best.
Fig. 2 is influence of the bacterium algae microballoon quantity to removal efficiency of nitrogen and phosphorus.The wherein accumulative removal rate of (a) ammonia nitrogen, (b) total phosphorus
Accumulative removal rate.From figure 2 it can be seen that 1500 microballoons are relative to other quantity from the point of view of the accumulative removal rate of ammonia nitrogen
Microballoon is best to the removal rate effect of ammonia nitrogen.From the point of view of the accumulative removal rate of total phosphorus, the removal rate of the more total phosphorus of microsphere particle amount
It is higher.2000 microballoons are all better than other three experiment samples for the removal effect of black smelly water total phosphorus.The removal of comprehensive nitrogen phosphorus
Rate, the black smelly optimal dispensing granule number of water for handling 250ml is 1500, that is to say, that reach best treatment effect, bacterium
The distribution density of algae microballoon is 6000/dm3。
Fig. 3 is influence of the temperature to bacterium algae microballoon removal efficiency of nitrogen and phosphorus in black smelly water.Wherein ammonia under (a) condition of different temperatures
Nitrogen removal efficiency variation, (b) total tp removal rate variation under condition of different temperatures.From figure 3, it can be seen that in 15 DEG C to 25 DEG C of temperature
Spend in range, the removal rate of nitrogen phosphorus also with temperature raising and increase, and when temperature is to 30 DEG C, the removal rate of nitrogen phosphorus is but
It has dropped, it may be possible to the physiological activity of algae and bacterium in Immobilized hyphae microballoon be caused to receive since temperature is excessively high
Limitation, and temperature is more than 30 DEG C, and the material for embedding bacterium algae understands some loosely.Immobilized hyphae at 25 DEG C in summary~30 DEG C
Microballoon is best to the removal rate of ammonia nitrogen and phosphorus
Specific embodiment
Below in conjunction with specific embodiment, the invention will be further described, but protection not thereby limiting the invention
Range.
Material employed in following embodiment and instrument are commercially available.
Embodiment 1:
The preparation method of Immobilized hyphae microballoon in the present embodiment, comprising the following steps:
1, under aseptic technique, chlorella vulgaris is inoculated into culture medium, under the conditions of 25 DEG C of temperature, carries out light
Dark time ratio 1:1 cycle alternation culture, intensity of illumination 3500Lux cultivate to increased logarithmic phase, obtain algae solution.Take training
It supports to the chlorella algae solution of logarithmic phase and is centrifuged 10min under the conditions of 3500r/min, be respectively washed, be centrifuged 2 times with distilled water, gone
It is spare at 5 DEG C except the inorganic salt and other material being attached on frustule.
2, the activated sludge for taking certain volume, is placed in centrifuge tube and stands 1h, is then centrifuged for (3500r/min, 10min)
Afterwards, liquid is discarded supernatant, twice with distilled water flushing, is concentrated spare after being centrifuged 1min again, is stored at 5 DEG C.
3, active carbon is passed through distilled water repeated flushing 3 times, baking oven is then placed in, in 50 DEG C~60 DEG C baking 12h, after grinding
60 meshes are crossed, it is spare.
4, it according to the biomass 2:1 of the bacterium of embedding and algae, takes in step (1) in the algae solution of 25ml and step (2)
The bacterium solution of 15ml carries out uniformly mixed, obtains mixed liquor.
5, prepare containing mass fraction be 0.6% sodium alginate and mass fraction be 11% PVA carrier solution, will
(4) mixed liquor obtained in is added in 60ml carrier solution, and instilling mass fraction with Syringe injector after mixing is 2%
Calcium chloride solution in, fix 2 hours, then take out after being crosslinked 2 hours, with deionized water desalination 2 hours, obtain Immobilized hyphae
Microballoon.
Embodiment 2:
Phycomycete biomass is produced respectively than being respectively 2:1,1:1,1:2, and it is micro- to make bacterium algae according to aforementioned process for fixation
Ball.Three diameter 10cm are taken, the black smelly water of 2L is added toward each container, according to 30% filler in the plexiglass box of high 30cm
Volumetric filling ratio adds phycomycete biomass than the bacterium algae microballoon for 2:1,1:1,1:2 into three containers respectively, is then placed in
It is cultivated under the conditions of 25 DEG C, light intensity 2500lx in incubator, daily manual shaking flask 2~3 times, every 12 hour water samplings measure ammonia
The concentration of nitrogen, total phosphorus.
If the best bacteria and algae amount ratio of Fig. 1, the Immobilized hyphae microballoon preparation are 2:1.
Embodiment 3:
The 500ml conical flask for taking the four black smelly water for filling 250ml, is charged with 500,1000,1500,2000 respectively
A bacterium algae microballoon carries out denitrogenation dephosphorizing processing, other conditions with embodiment 2, every 12 hour water samplings measurement ammonia nitrogen and total phosphorus
Concentration.
Such as Fig. 2, the Immobilized hyphae microballoon optimal distribution density that this is described after being computed is 6000/dm3。
Embodiment 4:
Take four diameter 10cm, the plexiglass box of high 30cm, be respectively placed in 15 DEG C, 20 DEG C, 25 DEG C, 30 DEG C 4 not
With at a temperature of, be added the bacterium algae microballoon of identical black smelly water and identical quantity into container, other conditions with embodiment 2,
The concentration of the every measurement of 12 hour water samplings ammonia nitrogen and total phosphorus.
Such as Fig. 3, it is 25 DEG C~30 DEG C which, which handles the most suitable temperature of black smelly water,.
The above is only a preferred embodiment of the present invention, protection scope of the present invention is not limited merely to above-mentioned implementation
Example.All technical solutions belonged under thinking of the present invention all belong to the scope of protection of the present invention.It is noted that for the art
Those of ordinary skill for, improvements and modifications without departing from the principle of the present invention, these improvements and modifications are also answered
It is considered as protection scope of the present invention.
Claims (10)
1. a kind of preparation method of Immobilized hyphae microballoon, it is characterised in that include the following steps:
Step 1: take culture to be centrifuged 10min under the conditions of 3500r/min to the chlorella algae solution of logarithmic phase, wash with distilled water,
Centrifugation is saved backup at 5 DEG C with removing the inorganic salt and other material being attached on frustule；
Step 2: taking the activated sludge of certain volume, be placed in centrifuge tube and stand 1h, liquid is discarded supernatant after centrifugation, after distilled water
It is centrifuged 1min again, is stored for future use at 5 DEG C after concentration；
Step 3: distilled water flushing active carbon is used, baking oven is then placed in, it is spare after grinding in 50 DEG C~60 DEG C dry 12h；
Step 4: a certain amount of PVA and sodium alginate being put into beaker, suitable distilled water is added, under 90 DEG C of water-baths completely
Dissolution naturally cools to 30 DEG C after being completely dissolved, form PVA-SA gel；
Step 5: the algae solution that step 1 obtains and the activated sludge for the concentration that step 2 obtains being mixed, obtained after being diluted with distilled water
Bacterium algae mixed liquor；The active carbon that step 3 obtains, mixing and absorption 10min, then by mixed liquor are added into gained bacterium algae mixed liquor
It is added in PVA-SA gel and stirs and evenly mixs, be added drop-wise to CaCl with syringe2In solution, filtered after fixing 2h, crosslinking 2h at room temperature
Microballoon out finally obtains Immobilized hyphae microballoon with deionized water desalination 2h.
2. preparation method according to claim 1, it is characterised in that:
In step 3,60 meshes are crossed after active carbon grinding.
3. preparation method according to claim 1, it is characterised in that:
In step 4, the mass concentration of PVA is 10%~12% in PVA-SA gel, the mass concentration of sodium alginate is 0.2%~
0.6%.
4. preparation method according to claim 1, it is characterised in that:
In step 5, phycomycete biomass ratio is 2:1 in bacterium algae mixed liquor.
5. preparation method according to claim 1, it is characterised in that:
In step 5, the dosage of active carbon accounts for the 0.8%~1.2% of bacterium algae mixed liquor gross mass.
6. preparation method according to claim 1, it is characterised in that:
In step 5, the volume ratio of bacterium algae mixed liquor and PVA-SA gel is 2:3.
7. preparation method according to claim 1, it is characterised in that:
In step 5, CaCl2The mass concentration of solution is 1.5%~2.5%.
8. the application for the Immobilized hyphae microballoon that any method is prepared in claim 1-7, it is characterised in that: be by institute
State processing of the Immobilized hyphae microballoon for sewage.
9. application according to claim 8, it is characterised in that:
The optimal distribution density of the Immobilized hyphae microballoon is 5500~6500/dm3。
10. application according to claim 8, it is characterised in that:
With the temperature of Immobilized hyphae microballoon processing sewage for 25 DEG C~30 DEG C.
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