CN105036344A - Method for biologically purifying aquaculture water body - Google Patents
Method for biologically purifying aquaculture water body Download PDFInfo
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- CN105036344A CN105036344A CN201510396566.5A CN201510396566A CN105036344A CN 105036344 A CN105036344 A CN 105036344A CN 201510396566 A CN201510396566 A CN 201510396566A CN 105036344 A CN105036344 A CN 105036344A
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- water body
- nitrobacteria
- sulfide
- aquaculture water
- marine algae
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Abstract
The invention relates to a method for purifying an aquaculture water body commonly by utilizing marine algae and microorganisms. According to the method, functional microorganisms of five different physiological groups, including bacillus subtilis, nitrifying bacteria, nitrite bacteria, photosynthetic bacteria and thiobacillus, are put into the aquaculture water body in a volume ratio of (1-5):(1-4):(1-6):(2-8):(1-5), and marine algae which is zostera marina or ulva is planted into the aquaculture water body at the same time. The method can be used for meeting the requirement for simultaneously purifying pollutant components in the aquaculture water body by utilizing marine algae and multiple bacteria.
Description
Technical field
The present invention relates to a kind of biological purification method of mariculture water body, particularly relate to and a kind of in aquaculture water, plant marine algae, and in aquaculture water, add the aquaculture water biological purification method of genus bacillus, nitrobacteria, nitrite bacteria, photosynthetic bacterium and sulfide-oxidizing enzyme.
Background technology
The growing standard of living that improve the people of aquaculture, no matter from cultured output or from cultivation scale, China is all the first in the world aquaculture big country.But China aquaculturist is target mainly with devoting exclusive attention to output with economic benefits in short, and cultivation density is too high, adds that environmental consciousness is thin, the farming disease harms is in the gesture increased the weight of year by year.Meanwhile, China's aquaculture is mostly hydrostatic cultivation, and highdensity cultivation causes the organic depositions such as a large amount of fishes and shrimps movements, residual bait water-bed.In the cultivation busy season, because temperature is high, cause heterotrophic microorganism amount reproduction, organism is decomposed, and discharges the objectionable impuritiess such as a large amount of ammonia, nitrite, thus affects fishes and shrimps growth, growth, causes fishes and shrimps disease.
All there is various drawback for the physics of purifying aquatic water, chemical process, and the microbial population existed in natural water body is not enough to decompose the organic loading in culturing pool, needs to seek eco-friendly cultivation water environment new purification method.
In recent years, someone carries out the research purified in aquaculture water by microbe application, absorbing by microbe, and pollutent is reduced the murder by poisoning of fishes and shrimps.
Aquatic plant system is also a kind of water body in-situ reparation preferably and control techniques, and particularly it has that cost is low, reusing is high, detergent power is strong and provide the plurality of advantages such as sheltered situation for marine organisms and receive much concern.
The present invention by planting marine algae in aquaculture water, and in aquaculture water, add genus bacillus, nitrobacteria, nitrite bacteria, photosynthetic bacterium and sulfide-oxidizing enzyme, build marine algae-microorganism symbiotic system, improve the biopurification ability to mariculture sewage.
Summary of the invention
The technical problem to be solved in the present invention is: the cogeneration system building a kind of marine algae and multiple-microorganism function yeast, to the purifying method that pollutant component multiple in mariculture water body is administered simultaneously, the multiple pollutant component in aquaculture water is made to reach the requirement simultaneously purified.
The technical scheme that the present invention solves this technical problem employing is: add genus bacillus, nitrobacteria, nitrite bacteria, photosynthetic bacterium and sulfide-oxidizing enzyme while of planting marine algae in aquaculture water.
Total injected volume of described genus bacillus, nitrobacteria, nitrite bacteria, photosynthetic bacterium and sulfide-oxidizing enzyme is the 0.001-0.01% of aquaculture water volume.
The planting density of described marine algae be 5 strains/square metre-20 strains/square metre.
The input volume ratio of described genus bacillus, nitrobacteria, nitrite bacteria, photosynthetic bacterium and sulfide-oxidizing enzyme is: genus bacillus: nitrobacteria: nitrite bacteria: photosynthetic bacterium: sulfide-oxidizing enzyme=(1-5): (1-4): (1-6): (2-8): (1-5).
Described genus bacillus, nitrobacteria, nitrite bacteria, photosynthetic bacterium and sulfide-oxidizing enzyme all directly screen and obtain from breeding pollution water body, and are cultivated the bacterium improvement culture method that screening obtains.
Described marine algae is sea tangle.
Described marine algae is sea lettuce.
Beneficial effect of the present invention:
Use the inventive method can reach to the multiple pollutant composition in aquaculture water the requirement simultaneously purified, and add genus bacillus, nitrobacteria, nitrite bacteria, photosynthetic bacterium and sulfide-oxidizing enzyme plant marine algae in aquaculture water while to the removal effect of pollutent apparently higher than being used alone microbial function bacterium purifying aquatic water.
Embodiment
The cultivation of embodiment 1 genus bacillus
1, Bacillus is separated and obtains from aquaculture wastewater, nutrient agar, nutrient broth medium that preparation is conventional.
2, the cultivation of bacterium
Aseptically be inoculated in by Bacillus on nutrient agar inclined-plane, then 37 DEG C of cultivation 24h in thermostat container, can see one deck white rounding, entire Bacillus colonies.At the cultured genus bacillus aseptic inoculation of nutrient agar in 100ml nutrient broth medium, will shake up, in 37 DEG C of shaking tables, cultivate 2-3 days, for subsequent use.
The cultivation of embodiment 2 nitrobacteria and nitrite bacteria
1, nitrobacteria and nitrite bacteria bacterial classification are separated and obtain from aquaculture wastewater, the nitrobacteria that preparation is conventional and nitrite bacteria liquid nutrient medium.
2, the cultivation of bacterium
The nitrobacteria of initial gross separation from aquaculture wastewater and nitrite bacteria to be inoculated in 100ml test tube 200 turns/min in 20-28 DEG C of shaking table, 3-5 days is cultivated in concussion, for subsequent use.
The cultivation of embodiment 3 photosynthetic bacterium
1, photosynthetic bacterium bacterial classification is separated and obtains from aquaculture wastewater, the photosynthetic bacterium solid that preparation is conventional and liquid nutrient medium.
2, the cultivation of bacterium
Photosynthetic bacterium bacterial classification is aseptically inoculated on photosynthetic bacterium solid medium, then under anaerobic, 24h is cultivated for 30 DEG C in thermostat container, will on photosynthetic bacterium solid medium cultured photosynthetic bacterium aseptic inoculation in 100ml photosynthetic bacterium liquid substratum, lower 30 DEG C of anaerobic condition cultivates 3-5 days, for subsequent use.
The cultivation of embodiment 4 sulfide-oxidizing enzyme
1, the liquid nutrient medium of formulating vulcanization bacterium
Na2S2.0g, Na2S2O35H2O5.0g, K2HPO42.0g, MgSO47H2O0.6g, NaHCO31.0g, FeSO47H2O0.01g, the pH value adjusting solution is 7.0-7.4, is mixed with the liquid nutrient medium of sulfide-oxidizing enzyme.
2, the cultivation of bacterium
Be inoculated into by the sulfide-oxidizing enzyme of initial gross separation in aquaculture wastewater in 100ml aforesaid liquid substratum, at 30 DEG C, 3-5 days is cultivated in shaking table concussion, for subsequent use.
The plantation of embodiment 5 sea tangle
1, the seawater of salinity 25 is prepared
In seawater, slowly add distilled water and constantly stir, period measures sea water salinity with salometer, until salinity reaches 25, stops adding distilled water.The seawater prepared is put into triangular flask, sterilizing 15 minutes, be cooled to 15 DEG C for subsequent use.
2, seed germination
Select the grass wrack seeds that head is full, color is Dark grey, with seawater flushing for several times, put into triangular flask, be advisable at the bottom of seed tiles bottle just, cultivate 14 days 15 DEG C of lucifuges.
3, seedling culture
With the natural materials jute rope that can degrade for folder seedling rope, two ends pendant is with sinker, take sea sand as matrix thing, cultivate in tank with being put in after germinating seed Homogeneous phase mixing, be the seawater of salinity 25 in tank, treat that seedling development has 1-2 sheet, spire length is 1-1.5cm, article 2, fibrous root length is 0.1-0.3cm, for subsequent use.
4, seedling replanting
Above-mentioned seedling is planted on seedling base, 15 DEG C, cultivate 15 days under illumination 5000 condition, mariculture area is excavated the pit of 3-3.5cm, cultivated sea tangle is imbedded wherein.
Embodiment 6 decontamination effect improving detects
After sea tangle is transplanted to mariculture area January, the genus bacillus that embodiment 1-4 is cultivated out, nitrobacteria, nitrite bacteria, photosynthetic bacterium, sulfide-oxidizing enzyme, getting 100ml in the mixing of 1:2:1:2:1 ratio accesses in 100L aquaculture wastewater, timing sampling surveys water body COD, ammonium nitrogen, total nitrogen, reduced sulfur compound and organic acid content, and result is as following table 1.
Embodiment 7 blank
Sea tangle removes sea tangle after being transplanted to mariculture area January.The genus bacillus that embodiment 1-4 is cultivated out, nitrobacteria, nitrite bacteria, photosynthetic bacterium, sulfide-oxidizing enzyme, getting 100ml in the mixing of 1:2:1:2:1 ratio accesses in 100L aquaculture wastewater, timing sampling surveys water body COD, ammonium nitrogen, total nitrogen, reduced sulfur compound and organic acid content, and result is as following table 2.
The inventive method can reach to the multiple pollutant composition in aquaculture water the requirement simultaneously purified, and adds genus bacillus, nitrobacteria, nitrite bacteria, photosynthetic bacterium and sulfide-oxidizing enzyme plant marine algae in aquaculture water while to the removal effect of pollutent apparently higher than being used alone microbial function bacterium purifying aquatic water.
It should be noted last that, above embodiment is only in order to illustrate technical scheme of the present invention and unrestricted, although with reference to preferred embodiment to invention has been detailed description, those of ordinary skill in the art is to be understood that, can modify to technical scheme of the present invention or equivalent replacement, and not departing from the spirit and scope of technical solution of the present invention, it all should be encompassed in the middle of right of the present invention.
Claims (7)
1. a biological purification method for mariculture water body, is characterized in that, in aquaculture water, plant marine algae, and in aquaculture water, add genus bacillus, nitrobacteria, nitrite bacteria, photosynthetic bacterium and sulfide-oxidizing enzyme.
2. the biological purification method of a kind of mariculture water body according to claim 1, is characterized in that, total injected volume of described genus bacillus, nitrobacteria, nitrite bacteria, photosynthetic bacterium and sulfide-oxidizing enzyme is the 0.001-0.01% of aquaculture water volume.
3. the biological purification method of a kind of mariculture water body according to claim 1, is characterized in that, the planting density of described marine algae be 5 strains/square metre-20 strains/square metre.
4. the biological purification method of a kind of mariculture water body according to claim 1, it is characterized in that, the input volume ratio of described genus bacillus, nitrobacteria, nitrite bacteria, photosynthetic bacterium and sulfide-oxidizing enzyme is: genus bacillus: nitrobacteria: nitrite bacteria: photosynthetic bacterium: sulfide-oxidizing enzyme=(1-5): (1-4): (1-6): (2-8): (1-5).
5. the biological purification method of a kind of mariculture water body according to claim 1, is characterized in that, described genus bacillus, nitrobacteria, nitrite bacteria, photosynthetic bacterium and sulfide-oxidizing enzyme all directly screen and obtain from breeding pollution water body.
6. the biological purification method of a kind of mariculture water body according to claim 1, is characterized in that, described marine algae is sea tangle.
7. the biological purification method of a kind of mariculture water body according to claim 1, is characterized in that, described marine algae is sea lettuce.
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108862605A (en) * | 2018-06-28 | 2018-11-23 | 普定县干河农业开发有限公司 | A kind of application of alga-bacterium symbiosis system pollutant in removal Pig raising wastewater |
| CN110818067A (en) * | 2019-11-28 | 2020-02-21 | 中国水产科学研究院渔业机械仪器研究所 | Nitrification bacteria and photosynthetic bacteria symbiotic prawn culture tail water treatment system |
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| Publication number | Priority date | Publication date | Assignee | Title |
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| CN1544355A (en) * | 2003-11-27 | 2004-11-10 | 天津南开戈德集团有限公司 | Aquacultural water body cleaning method using microorganism |
| CN101264980A (en) * | 2008-04-23 | 2008-09-17 | 中国水产科学研究院南海水产研究所 | Sea water culture environment multiple state position biological restoring method |
| CN101811775A (en) * | 2009-02-19 | 2010-08-25 | 中国环境科学研究院 | Composite bacteria-algae preparation method for purifying aquaculture waste water |
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2015
- 2015-07-08 CN CN201510396566.5A patent/CN105036344A/en active Pending
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1544355A (en) * | 2003-11-27 | 2004-11-10 | 天津南开戈德集团有限公司 | Aquacultural water body cleaning method using microorganism |
| CN101264980A (en) * | 2008-04-23 | 2008-09-17 | 中国水产科学研究院南海水产研究所 | Sea water culture environment multiple state position biological restoring method |
| CN101811775A (en) * | 2009-02-19 | 2010-08-25 | 中国环境科学研究院 | Composite bacteria-algae preparation method for purifying aquaculture waste water |
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108862605A (en) * | 2018-06-28 | 2018-11-23 | 普定县干河农业开发有限公司 | A kind of application of alga-bacterium symbiosis system pollutant in removal Pig raising wastewater |
| CN110818067A (en) * | 2019-11-28 | 2020-02-21 | 中国水产科学研究院渔业机械仪器研究所 | Nitrification bacteria and photosynthetic bacteria symbiotic prawn culture tail water treatment system |
| CN110818067B (en) * | 2019-11-28 | 2022-02-11 | 中国水产科学研究院渔业机械仪器研究所 | Nitrification bacteria and photosynthetic bacteria symbiotic prawn culture tail water treatment system |
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