CN108773912B - Microbial inoculum treatment process for livestock and poultry breeding sewage - Google Patents
Microbial inoculum treatment process for livestock and poultry breeding sewage Download PDFInfo
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- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F3/00—Biological treatment of water, waste water, or sewage
- C02F3/34—Biological treatment of water, waste water, or sewage characterised by the microorganisms used
- C02F3/341—Consortia of bacteria
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- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F3/00—Biological treatment of water, waste water, or sewage
- C02F3/34—Biological treatment of water, waste water, or sewage characterised by the microorganisms used
- C02F3/345—Biological treatment of water, waste water, or sewage characterised by the microorganisms used for biological oxidation or reduction of sulfur compounds
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- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F2305/00—Use of specific compounds during water treatment
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- Y02E—REDUCTION OF GREENHOUSE GAS [GHG] EMISSIONS, RELATED TO ENERGY GENERATION, TRANSMISSION OR DISTRIBUTION
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Abstract
The invention discloses a microbial inoculum treatment process of livestock and poultry breeding sewage, and a device thereof comprises the following steps: an aerobic strain tank, an anaerobic strain tank, a grid, a regulating tank, a flocculation tank, an anaerobic reaction tank, an aerobic aeration biological filter and a methane recovery device. The operation steps comprise: preparing a culture medium; culturing anaerobic bacteria; culturing aerobic bacteria; culturing flocculating bacteria; expanding, strengthening and domesticating the strains; the sewage passes through a grid and the pH is adjusted in an adjusting tank; entering a flocculation tank, and adding a flocculation microbial inoculum; allowing the sewage to enter an anaerobic reaction tank and stay for 8-10 hours, stirring, and recovering methane; sewage flows into an aerobic biological aerated filter and passes through three layers of profiled fiber nylon filter cloth; and (4) performing secondary filtration under the action of gravity, sterilizing by using an ultraviolet lamp, detecting, discharging, and returning to the anaerobic reaction tank if the standard is not met. The sewage treatment effect is good, no chemical reagent is added, and secondary pollution is avoided; the ventilation volume, the stirring volume, the sewage flow volume and the like are low, the odor and the residual sludge are less discharged, and the energy conservation and emission reduction are realized.
Description
Technical Field
The invention relates to a sewage treatment process, in particular to a microbial inoculum treatment process for livestock and poultry breeding sewage.
Background
The main pollutants of industrial sewage or municipal sewage are oxygen-consuming pollutants (organic substances such as carbohydrates, proteins, grease and lignin), toxic pollutants (inorganic anions, organic pesticides and the like), inorganic pollutants (acids, alkalis, salts and the like) and pathogen pollutants. The traditional sewage treatment method comprises the steps of stirring, aeration oxidation treatment, airless anaerobic treatment, precipitation and the like, wherein the oxidation treatment and the anaerobic treatment are essentially processes for degrading pollutants in water by microorganisms, usually natural strains or a small amount of artificially added strains are adopted, a part of chemical reagents are required to be added according to the types of the pollutants, and natural standing or flocculant addition precipitation is adopted in the precipitation process. The strain which is not screened and optimized has low sewage treatment efficiency, needs to be matched with a chemical reagent or a physical method, increases the possibility of secondary pollution, and also improves the time and the economic cost for sewage treatment. In addition, sewage treatment equipment is also important for sewage treatment effect, and most of sewage treatment equipment has small capacity, low operation efficiency, incomplete pollutant treatment and easy generation of odor and sludge with secondary pollution. Therefore, the superior strains for sewage treatment are screened, and the matched advanced sewage treatment equipment is developed, so that the capability and efficiency of the existing sewage treatment are improved, and the problem of sewage treatment is solved.
Chinese patent (CN201810012965.0) discloses a wastewater treatment device, which uses the mixture of anaerobic bacteria and facultative bacteria or aerobic bacteria as a microbial water purifying agent to treat wastewater to achieve good wastewater treatment effect, but uses inorganic flocculant and organic polymer flocculant as flocculant to cause secondary pollution to the environment. The industrial wastewater treatment process of Chinese patent (CN201611182630.0) uses mixed bacteria to treat wastewater, but because the bacteria are not domesticated, the capability of the bacteria to treat the wastewater is low, and the discharged wastewater can not meet the treatment standard easily.
Disclosure of Invention
Aiming at the defects of the prior art, the microbial inoculum treatment process for the livestock and poultry breeding sewage is provided, the sewage treatment effect is good, no chemical reagent is added, and the introduction of secondary pollution is avoided; recycling gases such as methane and the like for resource utilization; the ventilation volume, the stirring volume, the sewage flow volume and the like are low, the odor and the residual sludge are less discharged, and the energy conservation and emission reduction are realized.
The technical scheme of the invention is as follows:
(1) culturing anaerobic bacteria and aerobic bacteria: preparation of a culture medium: 0.3-0.5% of lactose, 0.3-0.8% of sodium hydrogen selenite, 0.3-0.8% of disodium hydrogen phosphate, 0.3-0.5% of L-cystine, 1% of peptone, 0.3-0.8% of potassium dihydrogen phosphate and the balance of water, adjusting the pH value to 7.0-7.5, and performing high-temperature sterilization at 110-130 ℃ for 20-25 min to obtain a culture medium A, B, C; inoculating anaerobic strains to the culture medium A in a sterile room according to a certain proportion, inoculating aerobic strains to the culture medium B in the sterile room according to a certain proportion, and culturing at the temperature of 24-32 ℃ for 24-50 hours;
(2) domesticating and strengthening culture of strains: respectively adding 20-30% of sludge obtained from a secondary sedimentation tank of a sewage treatment plant, 0.5% of sodium hydrogen selenite, 0.5% of lactose, 0.5% of potassium dihydrogen phosphate, 0.5-1% of vitamin complex, 1-2% of sodium gluconate, 1-2% of glyceryl monostearate, 1-2% of N, N-dimethylacetamide and the balance of wastewater into an aerobic strain tank and an anaerobic strain tank, and respectively adding cultured aerobic strains and anaerobic strains; firstly putting wastewater with the volume of 40-45% of the pool into a strain pool, and stirring; adding wastewater with the volume of 6-8% of the tank every day until the wastewater reaches a normal water level to obtain acclimatized and strengthened aerobic and anaerobic sludge, and putting the aerobic and anaerobic sludge and the anaerobic sludge into an anaerobic reaction tank together;
(3) culturing flocculating bacteria: inoculating a strain with flocculation capacity in a sterile room: inoculating pseudomonas mendocina and paecilomyces lilacinus at an inoculation ratio of 7:3 to a culture medium C, and culturing at 24-32 ℃ for 24-50 hours; then expanding and strengthening culture, wherein the expanding and strengthening culture medium comprises the following components: 1% of sucrose, 1% of agar, 1% of potassium nitrate, 0.5% of dipotassium hydrogen phosphate, 0.5% of magnesium sulfate, 0.5% of sodium chloride, 1-2% of sodium gluconate, 1-2% of glycerol monostearate, 1-2% of N, N-dimethylacetamide and the balance of water, and the pH value is adjusted to 7.0-7.5; starting a shaking table, wherein the culture temperature is 30 ℃, and the culture time is 48 h; centrifuging the strain culture medium after the amplification and strengthening culture at a high speed to obtain supernatant as a flocculating agent;
(4) the sewage passes through 8 layers of grids with grid gaps from coarse to fine to remove large-particle solid impurities; then the mixture enters an adjusting tank to adjust the pH value to 6-7.5;
(5) continuously feeding the sewage into a flocculation tank, adding a flocculation microbial inoculum with the volume of 2-5% of the sewage, stirring, and hydraulically staying for 7-9 hours to remove suspended matters and partial pollutants; an aeration device is arranged on one side wall body of the flocculation tank, a stirrer is arranged in the middle of the flocculation tank, and the bottom of the flocculation tank is a slope opposite to the water flow direction so as to collect and discharge sediments;
(6) enabling the supernatant of the flocculation tank to enter an anaerobic reaction tank, staying for 8-10 hours in a hydraulic manner, stirring, enabling aerobic bacteria to consume redundant oxygen to decompose pollutants, providing an anaerobic environment for anaerobic bacteria to enable the anaerobic bacteria to decompose the pollutants, and recycling methane generated in the process to a methane recycling tank connected with the anaerobic reaction tank;
(7) continuously flowing the sewage into the aerobic biological aerated filter from the bottom, and aerating on a supporting layer, wherein a filtering material of the supporting layer is cobblestones with the diameter of 3-8 cm; next, placing a first layer of cross-shaped nylon filter cloth, wherein the filter material is special-shaped ceramic with the length of 3-5 cm, the mixed microbial inoculum forms a biological membrane on the filter cloth to continuously decompose pollutants, and meanwhile, the structure of the filter cloth plays a role in filtering; the second layer is a trilobal woven nylon filter cloth, a filter material is special-shaped ceramic with the length of 1-3 cm, the third layer is a pentagonal woven nylon filter cloth, the filter material is activated carbon and is 5-100 meshes, and the filter material is from coarse to fine from bottom to top; the sewage is discharged into a filter tank from the top, is secondarily filtered under the action of gravity, passes through 60-200 meshes of active carbon, is coarse to fine from top to bottom, is double-layer nylon cloth with a five-pointed star weave method at the bottom layer, is disinfected by ultraviolet lamps arranged at the top and the bottom, is discharged after detection, and returns to an anaerobic reaction tank if the sewage does not reach the standard. The sewage treatment apparatus includes: an aerobic strain tank, an anaerobic strain tank, a grid, a regulating tank, a flocculation tank, an anaerobic reaction tank, an aerobic aeration biological filter and a methane recovery tank.
As a further illustration of the invention, the anaerobic bacteria is a combination of methanogenic bacilli, methanogenic cocci, and lactobacilli.
As a further illustration of the present invention, the inoculation ratio of methanogen, methanogen coccus, lactobacillus is 1:1: 2.
As a further illustration of the invention, the aerobic bacteria are a combination of sulfureted bacteria, phosphorus accumulating bacteria, hydrolytic acidification bacteria and yeast.
As a further illustration of the invention, the inoculation ratio of the sulfuration bacteria, the phosphorus accumulating bacteria, the hydrolytic acidification bacteria and the yeast is 2:2:3: 5.
As a further illustration of the invention, the sulfurating bacteria are one or two of thiobacillus thiooxidans, thiobacillus ferrooxidans and thiobacillus denitrificans.
As a further illustration of the invention, the phosphorus-accumulating bacteria are one or two of acinetobacter calcoaceticus, acinetobacter juniperi, denitrifying phosphorus-accumulating bacteria and rhodochrous bacteria.
As a further illustration of the invention, the hydrolytic acidification bacteria is one or two of bacillus cereus, clostridium amyloliquefaciens and bacteroides succinogenes.
As a further illustration of the invention, the stirring speed in the step (2) is 400 rpm-600 rpm; in the step (3), the shaking table speed is 100-150 r/min; in the step (5), the stirring speed is 200 rpm-400 rpm; in the step (6), the stirring speed is 300-500 rpm.
Anaerobic bacteria and aerobic bacteria coexist, and Suspended Solids (SS), Chemical Oxygen Demand (COD), Biochemical Oxygen Demand (BOD), Total Organic Carbon (TOC), Total Nitrogen (TN), Total Phosphorus (TP) and the like in water can be removed simultaneously;
domesticating and strengthening the strain to greatly enhance the digestion capacity of the strain so as to achieve the capacity of treating the sewage; the biological system has enzyme to accelerate the reaction, and the bacterial strain will produce oxido-reductase, hydrolase, deamination, decarboxylation, phosphorylation, dephosphorylation and other enzymes when acting on pollutant. Therefore, sodium gluconate, glyceryl monostearate and N, N-dimethylacetamide are added in the process as an enzyme production promoter, so that the growth of strains can be accelerated, stronger enzyme production capacity and digestion capacity are established in the growth forming process of the strains, the metabolism of microorganisms in a wastewater treatment system is promoted, the microorganisms are promoted to rapidly grow in a large quantity in a poor environment, good zoogloea is formed, the efficiency of degrading organic pollutants by the microorganisms is improved, and the wastewater treatment effect is improved. And various microorganisms can effectively and synergistically play a role by prolonging the food chain and improving the circulation efficiency of the food chain, so that pollutants are degraded more thoroughly, and the load impact resistance of the system is improved. The addition of sodium gluconate, glyceryl monostearate and N, N-dimethylacetamide can improve the activity of extracellular enzyme, improve the permeability of cells, and enable more enzyme to permeate cell membranes from the cells to enter fermentation liquor, thereby being beneficial to breaking the feedback regulation of intracellular enzyme synthesis and improving the yield of enzyme in the fermentation liquor. The components used in the invention are all environment-friendly and nontoxic, do not poison the strains and cause secondary pollution to the environment, and can provide certain nutrition and energy for the strains.
The invention selects the pseudomonas mendocina and the paecilomyces lilacinus which not only have flocculation function but also can decompose pollutants, especially protein, fat, strains which are harmful to animal oil, and do not cause harm to water bodies and organisms, the effect of the joint application according to a certain proportion is better than that of the single application, and because the flaghair of the actinomycetes is long and coiled, the pseudomonas and the ascomycetes can fall on the hyphae to form a hollow hypha mass with pores, and the contact area with the pollutants is larger; the selected strains of the invention can also resist certain viruses and bacteria and play a certain role in disinfection.
Combustible gas such as methane and the like can be generated by anaerobic bacteria fermentation, and resource utilization can be realized through recovery;
the microbial inoculum is adsorbed on a substrate to form a biological membrane, the number, the types and the thickness of the biological membrane of microorganisms are increased along with the growth and the increment of the microorganisms, and an organic pollutant-microorganism-protozoan food chain can be formed to promote the degradation of organic matters; the nylon cloth with cross, trilobal and pentagram weaves is used as the filter membrane and has larger friction coefficient, so the fabric is coarse and thick, the suede surface can keep plump and upright, and has better mechanical bulkiness, and the nylon cloth can not hook and jump threads and break holes after long-term use and has long service life. The groove can effectively resist the water flow scouring in the horizontal direction (water flow in and out) and the vertical direction (generated by aeration), and the strains are easy to parasitize and propagate in the initial stage of film formation; the edges and corners of the fibers have better cutting effect on bubbles, the utilization rate of dissolved oxygen is improved, and the oxygen exposure power consumption is reduced.
The sewage treatment equipment and the sewage treatment process can reduce ventilation volume, stirring volume, sewage flow volume and the like, reduce odor and residual sludge discharge, and realize energy conservation and emission reduction.
Detailed Description
Example 1:
(1) culturing anaerobic bacteria and aerobic bacteria: preparation of a culture medium: 0.5% of lactose, 0.8% of sodium hydrogen selenite, 0.5% of disodium hydrogen phosphate, 0.4% of L-cystine, 1% of peptone, 0.7% of potassium dihydrogen phosphate and the balance of water, adjusting the pH value to 7, and performing high-temperature sterilization at 120 ℃ for 21min to obtain a culture medium A, B, C; the method comprises the steps of inoculating methanogen, methanococcus and lactobacillus to a culture medium A in a sterile room according to the ratio of 1:1:2, inoculating thiobacillus thioparus, thiobacillus ferrooxidans, acinetobacter junii, clostridium amyloliquefaciens and yeast to a culture medium B in the sterile room according to the ratio of 1.5:0.5:2:3:5, culturing at the temperature of 30 ℃ for 45 hours;
(2) domesticating and strengthening culture of strains: respectively adding 30% of sludge obtained from a secondary sedimentation tank of a sewage treatment plant, 0.5% of sodium hydrogen selenite, 0.5% of lactose, 0.5% of potassium dihydrogen phosphate, 0.5% of vitamin complex, 2% of sodium gluconate, 1% of glyceryl monostearate, 2% of N, N-dimethylacetamide and the balance of wastewater into an aerobic strain tank and an anaerobic strain tank, and respectively adding cultured aerobic strains and anaerobic strains; firstly, adding wastewater with the volume of 40 percent of the tank into a strain tank, and stirring at the stirring speed of 450 rpm; adding wastewater with the volume of 6 percent of the tank every day until reaching the normal water level to obtain acclimatized and strengthened aerobic and anaerobic sludge, and putting the acclimatized and strengthened aerobic and anaerobic sludge into an anaerobic reaction tank together;
(3) culturing flocculating bacteria: inoculating a strain with flocculation capacity in a sterile room: inoculating pseudomonas mendocina and paecilomyces lilacinus at the ratio of 7:3 to a culture medium C, and culturing at 26 ℃ for 50 hours; then expanding and strengthening culture, wherein the expanding and strengthening culture medium comprises the following components: 1% of sucrose, 1% of agar, 1% of potassium nitrate, 0.5% of dipotassium hydrogen phosphate, 0.5% of magnesium sulfate, 0.5% of sodium chloride, 2% of sodium gluconate, 1% of glycerol monostearate, 1% of N, N-dimethylacetamide and the balance of water, and adjusting the pH value to 7; starting a shaking table, wherein the speed of the shaking table is 100r/min, the culture temperature is 30 ℃, and the culture time is 42 h; centrifuging the strain culture medium after the amplification and strengthening culture at a high speed to obtain supernatant as a flocculating agent;
(4) the sewage passes through 8 layers of grids with grid gaps from coarse to fine to remove large-particle solid impurities; then the mixture enters an adjusting tank to adjust the pH value to 6;
(5) continuously feeding the sewage into a flocculation tank, adding a flocculation microbial inoculum with the sewage volume of 3%, and stirring at the stirring speed of 300 rpm; the hydraulic retention time is 7 hours to remove suspended matters and partial pollutants; an aeration device is arranged on one side wall body of the flocculation tank, a stirrer is arranged in the middle of the flocculation tank, and the bottom of the flocculation tank is a slope opposite to the water flow direction so as to collect and discharge sediments;
(6) enabling the supernatant of the flocculation tank to enter an anaerobic reaction tank, staying for 8 hours in a hydraulic manner, stirring at the stirring speed of 400rpm, enabling aerobic bacteria to consume redundant oxygen to decompose pollutants, providing an anaerobic environment for anaerobic bacteria to enable the anaerobic bacteria to decompose the pollutants, and recovering methane generated in the process to a methane recovery tank connected with the anaerobic reaction tank;
(7) continuously flowing the sewage into the aerobic biological aerated filter from the bottom, and aerating on a supporting layer, wherein a filtering material of the supporting layer is cobblestones with the diameter of 3-8 cm; next, placing a first layer of cross-shaped nylon filter cloth, wherein the filter material is special-shaped ceramic with the length of 3-5 cm, the mixed microbial inoculum forms a biological membrane on the filter cloth to continuously decompose pollutants, and meanwhile, the structure of the filter cloth plays a role in filtering; the second layer is a trilobal weaving nylon filter cloth, and the filter material is special-shaped ceramic with the length of 1-3 cm; the third layer is pentagonal weaving nylon filter cloth, the filter material is activated carbon, is 5-100 meshes and is from coarse to fine from bottom to top; the sewage is discharged into a filter tank from the top, is secondarily filtered under the action of gravity, passes through 60-200 meshes of active carbon, is coarse to fine from top to bottom, is double-layer nylon cloth with a five-pointed star weave method at the bottom layer, is disinfected by ultraviolet lamps arranged at the top and the bottom, is discharged after detection, and returns to an anaerobic reaction tank if the sewage does not reach the standard.
Example 2:
(1) culturing anaerobic bacteria and aerobic bacteria: preparation of a culture medium: 0.5% of lactose, 0.4% of sodium hydrogen selenite, 0.3% of disodium hydrogen phosphate, 0.5% of L-cystine, 1% of peptone, 0.8% of potassium dihydrogen phosphate and the balance of water, adjusting the pH value to 7, and performing high-temperature sterilization at 125 ℃ for 23min to obtain a culture medium A, B, C; inoculating methanogen, methanococcus and lactobacillus to the culture medium A in a sterile room according to a ratio of 1:1:2, inoculating thiobacillus thiooxidans, thiobacillus denitrificans, denitrifying phosphorus accumulating bacteria, bacteroides succinate and yeast to the culture medium B in the sterile room according to a ratio of 1:1:2:3:5, and culturing at 28 ℃ for 35 hours;
(2) domesticating and strengthening culture of strains: respectively adding 20% of sludge obtained from a secondary sedimentation tank of a sewage treatment plant, 0.5% of sodium hydrogen selenite, 0.5% of lactose, 0.5% of potassium dihydrogen phosphate, 1% of compound vitamin, 1% of sodium gluconate, 2% of glyceryl monostearate, 1% of N, N-dimethylacetamide and the balance of wastewater into an aerobic strain tank and an anaerobic strain tank, and respectively adding cultured aerobic strains and anaerobic strains; firstly, wastewater with the volume of 42 percent of the tank is put into the strain tank and stirred at the stirring speed of 550 rpm; adding wastewater with the volume of 7 percent of the tank every day until reaching the normal water level to obtain acclimatized and strengthened aerobic and anaerobic sludge, and putting the acclimatized and strengthened aerobic and anaerobic sludge into an anaerobic reaction tank together;
(3) culturing flocculating bacteria: inoculating a strain with flocculation capacity in a sterile room: inoculating pseudomonas mendocina and paecilomyces lilacinus at the ratio of 7:3 to a culture medium C, and culturing at 28 ℃ for 47 hours; then expanding and strengthening culture, wherein the expanding and strengthening culture medium comprises the following components: 1% of sucrose, 1% of agar, 1% of potassium nitrate, 0.5% of dipotassium hydrogen phosphate, 0.5% of magnesium sulfate, 0.5% of sodium chloride, 1% of sodium gluconate, 2% of glycerol monostearate, 2% of N, N-dimethylacetamide and the balance of water, and adjusting the pH value to 7.5; starting a shaking table, wherein the speed of the shaking table is 150r/min, the culture temperature is 28 ℃, and the culture time is 48 h; centrifuging the strain culture medium after the amplification and strengthening culture at a high speed to obtain supernatant as a flocculating agent;
(4) the sewage passes through 8 layers of grids with grid gaps from coarse to fine to remove large-particle solid impurities; then the mixture enters an adjusting tank to adjust the pH value to 7;
(5) continuously feeding the sewage into a flocculation tank, adding a flocculation microbial inoculum with the volume of 4% of the sewage, and stirring at the stirring speed of 350 rpm; the hydraulic retention is carried out for 8 hours to remove suspended matters and partial pollutants; an aeration device is arranged on one side wall body of the flocculation tank, a stirrer is arranged in the middle of the flocculation tank, and the bottom of the flocculation tank is a slope opposite to the water flow direction so as to collect and discharge sediments;
(6) enabling the supernatant of the flocculation tank to enter an anaerobic reaction tank, staying for 9 hours in a hydraulic manner, stirring at the stirring speed of 450rpm, enabling aerobic bacteria to consume redundant oxygen to decompose pollutants, providing an anaerobic environment for anaerobic bacteria to enable the anaerobic bacteria to decompose the pollutants, and recovering methane generated in the process to a methane recovery tank connected with the anaerobic reaction tank;
(7) continuously flowing the sewage into the aerobic biological aerated filter from the bottom, and aerating on a supporting layer, wherein a filtering material of the supporting layer is cobblestones with the diameter of 3-8 cm; next, placing a first layer of cross-shaped nylon filter cloth, wherein the filter material is special-shaped ceramic with the length of 3-5 cm, the mixed microbial inoculum forms a biological membrane on the filter cloth to continuously decompose pollutants, and meanwhile, the structure of the filter cloth plays a role in filtering; the second layer is a trilobal weaving nylon filter cloth, and the filter material is special-shaped ceramic with the length of 1-3 cm; the third layer is pentagonal weaving nylon filter cloth, the filter material is activated carbon, is 5-100 meshes and is from coarse to fine from bottom to top; the sewage is discharged into a filter tank from the top, is secondarily filtered under the action of gravity, passes through 60-200 meshes of active carbon, is coarse to fine from top to bottom, is double-layer nylon cloth with a five-pointed star weave method at the bottom layer, is disinfected by ultraviolet lamps arranged at the top and the bottom, is discharged after detection, and returns to an anaerobic reaction tank if the sewage does not reach the standard.
Example 3:
(1) culturing anaerobic bacteria and aerobic bacteria: preparation of a culture medium: 0.4% of lactose, 0.6% of sodium hydrogen selenite, 0.7% of disodium hydrogen phosphate, 0.3% of L-cystine, 1% of peptone, 0.4% of potassium dihydrogen phosphate and the balance of water, adjusting the pH value to 7.5, and performing high-temperature sterilization at 115 ℃ for 25min to obtain a culture medium A, B, C; inoculating methanogen, methanococcus methanogenes and lactobacillus to a culture medium A in a sterile room according to the ratio of 1:1:2, inoculating thiobacillus denitrificans, rhodochrous bacteria, bacillus cereus and yeast to a culture medium B in the sterile room according to the ratio of 2:2:3:5, and culturing at the temperature of 25 ℃ for 40 hours;
(2) domesticating and strengthening culture of strains: respectively adding 28% of sludge obtained from a secondary sedimentation tank of a sewage treatment plant, 0.5% of sodium hydrogen selenite, 0.5% of lactose, 0.5% of potassium dihydrogen phosphate, 1% of compound vitamin, 2% of sodium gluconate, 1% of glyceryl monostearate, 1% of N, N-dimethylacetamide and the balance of wastewater into an aerobic strain tank and an anaerobic strain tank, and respectively adding cultured aerobic strains and anaerobic strains; firstly putting wastewater with the volume of 45 percent of the tank into a strain tank, and stirring at the stirring speed of 500 rpm; adding wastewater with the volume of 8 percent of the tank every day until reaching the normal water level to obtain acclimatized and strengthened aerobic and anaerobic sludge, and putting the acclimatized and strengthened aerobic and anaerobic sludge into an anaerobic reaction tank together;
(3) culturing flocculating bacteria: inoculating a strain with flocculation capacity in a sterile room: inoculating pseudomonas mendocina and paecilomyces lilacinus at the ratio of 7:3 to a culture medium C, and culturing at the temperature of 30 ℃ for 36 hours; then expanding and strengthening culture, wherein the expanding and strengthening culture medium comprises the following components: 1% of sucrose, 1% of agar, 1% of potassium nitrate, 0.5% of dipotassium hydrogen phosphate, 0.5% of magnesium sulfate, 0.5% of sodium chloride, 2% of sodium gluconate, 1% of glycerol monostearate, 2% of N, N-dimethylacetamide and the balance of water, and adjusting the pH value to 7.5; starting a shaking table, wherein the speed of the shaking table is 120r/min, the culture temperature is 26 ℃, and the culture time is 41 h; centrifuging the strain culture medium after the amplification and strengthening culture at a high speed to obtain supernatant as a flocculating agent;
(4) the sewage passes through 8 layers of grids with grid gaps from coarse to fine to remove large-particle solid impurities; then the mixture enters an adjusting tank to adjust the pH value to 7.5;
(5) continuously feeding the sewage into a flocculation tank, adding a flocculation microbial inoculum with the sewage volume of 2%, and stirring at the stirring speed of 250 rpm; hydraulic retention is carried out for 9 hours to remove suspended matters and partial pollutants; an aeration device is arranged on one side wall body of the flocculation tank, a stirrer is arranged in the middle of the flocculation tank, and the bottom of the flocculation tank is a slope opposite to the water flow direction so as to collect and discharge sediments;
(6) enabling the supernatant of the flocculation tank to enter an anaerobic reaction tank, staying for 9.5 hours in a hydraulic manner, stirring at the stirring speed of 350rpm, enabling aerobic bacteria to consume redundant oxygen to decompose pollutants, providing an anaerobic environment for anaerobic bacteria to enable the anaerobic bacteria to decompose the pollutants, and recovering methane generated in the process to a methane recovery tank connected with the anaerobic reaction tank;
(7) continuously flowing the sewage into the aerobic biological aerated filter from the bottom, and aerating on a supporting layer, wherein a filtering material of the supporting layer is cobblestones with the diameter of 3-8 cm; next, placing a first layer of cross-shaped nylon filter cloth, wherein the filter material is special-shaped ceramic with the length of 3-5 cm, the mixed microbial inoculum forms a biological membrane on the filter cloth to continuously decompose pollutants, and meanwhile, the structure of the filter cloth plays a role in filtering; the second layer is a trilobal weaving nylon filter cloth, and the filter material is special-shaped ceramic with the length of 1-3 cm; the third layer is pentagonal weaving nylon filter cloth, the filter material is activated carbon, is 5-100 meshes and is from coarse to fine from bottom to top; the sewage is discharged into a filter tank from the top, is secondarily filtered under the action of gravity, passes through 60-200 meshes of active carbon, is coarse to fine from top to bottom, is double-layer nylon cloth with a five-pointed star weave method at the bottom layer, is disinfected by ultraviolet lamps arranged at the top and the bottom, is discharged after detection, and returns to an anaerobic reaction tank if the sewage does not reach the standard.
Example 4:
(1) culturing anaerobic bacteria and aerobic bacteria: preparation of a culture medium: 0.5% of lactose, 0.4% of sodium hydrogen selenite, 0.6% of disodium hydrogen phosphate, 0.3% of L-cystine, 1% of peptone, 0.7% of potassium dihydrogen phosphate and the balance of water, adjusting the pH value to 7, and performing high-temperature sterilization at 110 ℃ for 24min to obtain a culture medium A, B, C; inoculating methanogen, methanococcus and lactobacillus to a culture medium A in a sterile room according to a ratio of 1:1:2, inoculating thiobacillus thiooxidans, denitrifying phosphorus accumulating bacteria, clostridium amyloliquefaciens, bacteroides succinogenes and yeast to a culture medium B in the sterile room according to a ratio of 2:2:1:2:5, and culturing at 32 ℃ for 50 hours;
(2) domesticating and strengthening culture of strains: 25% of sludge obtained from a secondary sedimentation tank of a sewage treatment plant, 0.5% of sodium hydrogen selenite, 0.5% of lactose, 0.5% of potassium dihydrogen phosphate, 0.5% of vitamin complex, 2% of sodium gluconate, 1% of glyceryl monostearate, 2% of N, N-dimethylacetamide and the balance of wastewater are respectively added into an aerobic strain tank and an anaerobic strain tank, and then cultured aerobic strains and anaerobic strains are respectively added; firstly putting wastewater with the volume of 44% of the pool volume into a strain pool, and stirring at the stirring speed of 600 rpm; adding wastewater with the volume of 7 percent of the tank every day until reaching the normal water level to obtain acclimatized and strengthened aerobic and anaerobic sludge, and putting the acclimatized and strengthened aerobic and anaerobic sludge into an anaerobic reaction tank together;
(3) culturing flocculating bacteria: inoculating a strain with flocculation capacity in a sterile room: inoculating pseudomonas mendocina and paecilomyces lilacinus at the ratio of 7:3 to a culture medium C, and culturing at the temperature of 31 ℃ for 29 hours; then expanding and strengthening culture, wherein the expanding and strengthening culture medium comprises the following components: 1% of sucrose, 1% of agar, 1% of potassium nitrate, 0.5% of dipotassium hydrogen phosphate, 0.5% of magnesium sulfate, 0.5% of sodium chloride, 1% of sodium gluconate, 1% of glycerol monostearate, 2% of N, N-dimethylacetamide and the balance of water, and adjusting the pH value to 7; starting a shaking table, wherein the speed of the shaking table is 120r/min, the culture temperature is 32 ℃, and the culture time is 45 h; centrifuging the strain culture medium after the amplification and strengthening culture at a high speed to obtain supernatant as a flocculating agent;
(4) the sewage passes through 8 layers of grids with grid gaps from coarse to fine to remove large-particle solid impurities; then the mixture enters an adjusting tank to adjust the pH value to 7;
(5) continuously feeding the sewage into a flocculation tank, adding a flocculation microbial inoculum with the sewage volume of 5%, and stirring at the stirring speed of 200 rpm; hydraulic retention is carried out for 8.5 hours to remove suspended matters and partial pollutants; an aeration device is arranged on one side wall body of the flocculation tank, a stirrer is arranged in the middle of the flocculation tank, and the bottom of the flocculation tank is a slope opposite to the water flow direction so as to collect and discharge sediments;
(6) enabling the supernatant of the flocculation tank to enter an anaerobic reaction tank, staying for 8.5 hours in a hydraulic manner, stirring at the stirring speed of 300rpm, enabling aerobic bacteria to consume redundant oxygen to decompose pollutants, providing an anaerobic environment for anaerobic bacteria to enable the anaerobic bacteria to decompose the pollutants, and recovering methane generated in the process to a methane recovery tank connected with the anaerobic reaction tank;
(7) continuously flowing the sewage into the aerobic biological aerated filter from the bottom, and aerating on a supporting layer, wherein a filtering material of the supporting layer is cobblestones with the diameter of 3-8 cm; next, placing a first layer of cross-shaped nylon filter cloth, wherein the filter material is special-shaped ceramic with the length of 3-5 cm, the mixed microbial inoculum forms a biological membrane on the filter cloth to continuously decompose pollutants, and meanwhile, the structure of the filter cloth plays a role in filtering; the second layer is a trilobal weaving nylon filter cloth, and the filter material is special-shaped ceramic with the length of 1-3 cm; the third layer is pentagonal weaving nylon filter cloth, the filter material is activated carbon, is 5-100 meshes and is from coarse to fine from bottom to top; the sewage is discharged into a filter tank from the top, is secondarily filtered under the action of gravity, passes through 60-200 meshes of active carbon, is coarse to fine from top to bottom, is double-layer nylon cloth with a five-pointed star weave method at the bottom layer, is disinfected by ultraviolet lamps arranged at the top and the bottom, is discharged after detection, and returns to an anaerobic reaction tank if the sewage does not reach the standard.
Example 5:
(1) culturing anaerobic bacteria and aerobic bacteria: preparation of a culture medium: 0.3% of lactose, 0.8% of sodium hydrogen selenite, 0.5% of disodium hydrogen phosphate, 0.6% of L-cystine, 1% of peptone, 0.4% of potassium dihydrogen phosphate and the balance of water, adjusting the pH value to 7, and performing high-temperature sterilization at 130 ℃ for 20min to obtain a culture medium A, B, C; inoculating methanogen, methanococcus and lactobacillus to a culture medium A in a sterile room according to the ratio of 1:1:2, inoculating sulfur-containing bacteria such as thiobacillus ferrooxidans, thiobacillus denitrificans, acinetobacter calcoaceticus, bacillus cereus and saccharomycetes in the sterile room according to the ratio of 1:1:2:3:5, and culturing for 25 hours at the culture temperature of 24 ℃;
(2) domesticating and strengthening culture of strains: 22% of sludge obtained from a secondary sedimentation tank of a sewage treatment plant, 0.5% of sodium hydrogen selenite, 0.5% of lactose, 0.5% of potassium dihydrogen phosphate, 1% of compound vitamin, 1% of sodium gluconate, 2% of glyceryl monostearate, 2% of N, N-dimethylacetamide and the balance of wastewater are respectively added into an aerobic strain tank and an anaerobic strain tank, and then cultured aerobic strains and anaerobic strains are respectively added; firstly putting wastewater with the volume of 43 percent of the tank into a strain tank, and stirring at the stirring speed of 400 rpm; adding wastewater with the volume of 8 percent of the tank every day until reaching the normal water level to obtain acclimatized and strengthened aerobic and anaerobic sludge, and putting the acclimatized and strengthened aerobic and anaerobic sludge into an anaerobic reaction tank together;
(3) culturing flocculating bacteria: inoculating a strain with flocculation capacity in a sterile room: inoculating pseudomonas mendocina and paecilomyces lilacinus at the ratio of 7:3 to a culture medium C, and culturing at the temperature of 27 ℃ for 40 hours; then expanding and strengthening culture, wherein the expanding and strengthening culture medium comprises the following components: 1% of sucrose, 1% of agar, 1% of potassium nitrate, 0.5% of dipotassium hydrogen phosphate, 0.5% of magnesium sulfate, 0.5% of sodium chloride, 2% of sodium gluconate, 2% of glycerol monostearate, 2% of N, N-dimethylacetamide and the balance of water, and adjusting the pH value to 7; starting a shaking table, wherein the speed of the shaking table is 110r/min, the culture temperature is 27 ℃, and the culture time is 50 h; centrifuging the strain culture medium after the amplification and strengthening culture at a high speed to obtain supernatant as a flocculating agent;
(4) the sewage passes through 8 layers of grids with grid gaps from coarse to fine to remove large-particle solid impurities; then the mixture enters an adjusting tank to adjust the pH value to 6;
(5) continuously feeding the sewage into a flocculation tank, adding a flocculation microbial inoculum with the sewage volume of 3%, and stirring at the stirring speed of 400 rpm; hydraulic retention is carried out for 8.5 hours to remove suspended matters and partial pollutants; an aeration device is arranged on one side wall body of the flocculation tank, a stirrer is arranged in the middle of the flocculation tank, and the bottom of the flocculation tank is a slope opposite to the water flow direction so as to collect and discharge sediments;
(6) enabling the supernatant of the flocculation tank to enter an anaerobic reaction tank, staying for 10 hours in a hydraulic manner, stirring at the stirring speed of 500rpm, enabling aerobic bacteria to consume redundant oxygen to decompose pollutants, providing an anaerobic environment for anaerobic bacteria to enable the anaerobic bacteria to decompose the pollutants, and recovering methane generated in the process to a methane recovery tank connected with the anaerobic reaction tank;
(7) continuously flowing the sewage into the aerobic biological aerated filter from the bottom, and aerating on a supporting layer, wherein a filtering material of the supporting layer is cobblestones with the diameter of 3-8 cm; next, placing a first layer of cross-shaped nylon filter cloth, wherein the filter material is special-shaped ceramic with the length of 3-5 cm, the mixed microbial inoculum forms a biological membrane on the filter cloth to continuously decompose pollutants, and meanwhile, the structure of the filter cloth plays a role in filtering; the second layer is a trilobal weaving nylon filter cloth, and the filter material is special-shaped ceramic with the length of 1-3 cm; the third layer is pentagonal weaving nylon filter cloth, the filter material is activated carbon, is 5-100 meshes and is from coarse to fine from bottom to top; the sewage is discharged into a filter tank from the top, is secondarily filtered under the action of gravity, passes through 60-200 meshes of active carbon, is coarse to fine from top to bottom, is double-layer nylon cloth with a five-pointed star weave method at the bottom layer, is disinfected by ultraviolet lamps arranged at the top and the bottom, is discharged after detection, and returns to an anaerobic reaction tank if the sewage does not reach the standard.
Claims (9)
1. A microbial inoculum treatment process for livestock and poultry breeding sewage is characterized by comprising the following steps:
(1) culturing anaerobic bacteria and aerobic bacteria: preparation of a culture medium: 0.3-0.5% of lactose, 0.3-0.8% of sodium hydrogen selenite, 0.3-0.8% of disodium hydrogen phosphate, 0.3-0.5% of L-cystine, 1% of peptone, 0.3-0.8% of potassium dihydrogen phosphate and the balance of water, adjusting the pH value to 7.0-7.5, and performing high-temperature sterilization at 110-130 ℃ for 20-25 min to obtain a culture medium A, B, C; inoculating anaerobic strains to the culture medium A in a sterile room according to a certain proportion, inoculating aerobic strains to the culture medium B in the sterile room according to a certain proportion, and culturing at the temperature of 24-32 ℃ for 24-50 hours;
(2) domesticating and strengthening culture of strains: respectively adding 20-30% of sludge obtained from a secondary sedimentation tank of a sewage treatment plant, 0.5% of sodium hydrogen selenite, 0.5% of lactose, 0.5% of potassium dihydrogen phosphate, 0.5-1% of vitamin complex, 1-2% of sodium gluconate, 1-2% of glyceryl monostearate, 1-2% of N, N-dimethylacetamide and the balance of wastewater into an aerobic strain tank and an anaerobic strain tank, and respectively adding cultured aerobic strains and anaerobic strains; firstly putting wastewater with the volume of 40-45% of the pool into a strain pool, and stirring; adding wastewater with the volume of 6-8% of the tank every day until the wastewater reaches a normal water level to obtain acclimatized and strengthened aerobic and anaerobic sludge, and putting the aerobic and anaerobic sludge and the anaerobic sludge into an anaerobic reaction tank together; the wastewater is livestock and poultry breeding wastewater;
(3) culturing flocculating bacteria: inoculating a strain with flocculation capacity in a sterile room: pseudomonas mendocina and Paecilomyces lilacinus with the inoculation ratio of 7:3 to the culture medium C, wherein the culture temperature is 24-32 ℃, and the culture time is 24-50 hours; then expanding and strengthening culture, wherein the expanding and strengthening culture medium comprises the following components: 1% of sucrose, 1% of agar, 1% of potassium nitrate, 0.5% of dipotassium hydrogen phosphate, 0.5% of magnesium sulfate, 0.5% of sodium chloride, 1-2% of sodium gluconate, 1-2% of glycerol monostearate, 1-2% of N, N-dimethylacetamide and the balance of water, and the pH value is adjusted to 7.0-7.5; starting a shaking table, wherein the culture temperature is 30 ℃, and the culture time is 48 h; centrifuging the strain culture medium after the amplification and strengthening culture at a high speed to obtain supernatant as a flocculating agent;
(4) the sewage passes through 8 layers of grids with grid gaps from coarse to fine to remove large-particle solid impurities; then the mixture enters an adjusting tank to adjust the pH value to 6-7.5;
(5) continuously feeding the sewage into a flocculation tank, adding a flocculation microbial inoculum with the volume of 2-5% of the sewage, stirring, and hydraulically staying for 7-9 hours to remove suspended matters and partial pollutants; an aeration device is arranged on one side wall body of the flocculation tank, a stirrer is arranged in the middle of the flocculation tank, and the bottom of the flocculation tank is a slope opposite to the water flow direction so as to collect and discharge sediments;
(6) enabling the supernatant of the flocculation tank to enter an anaerobic reaction tank, staying for 8-10 hours in a hydraulic manner, stirring, enabling aerobic bacteria to consume redundant oxygen to decompose pollutants, providing an anaerobic environment for anaerobic bacteria to enable the anaerobic bacteria to decompose the pollutants, and recycling methane generated in the process to a methane recycling tank connected with the anaerobic reaction tank;
(7) continuously flowing the sewage into the aerobic biological aerated filter from the bottom, and aerating on a supporting layer, wherein a filtering material of the supporting layer is cobblestones with the diameter of 3-8 cm; next, placing a first layer of cross-shaped nylon filter cloth, wherein the filter material is special-shaped ceramic with the length of 3-5 cm, the mixed microbial inoculum forms a biological membrane on the filter cloth to continuously decompose pollutants, and meanwhile, the structure of the filter cloth plays a role in filtering; the second layer is a trilobal weaving nylon filter cloth, and the filter material is special-shaped ceramic with the length of 1-3 cm; the third layer is pentagonal weaving nylon filter cloth, the filter material is activated carbon, is 5-100 meshes and is from coarse to fine from bottom to top; discharging sewage into a filtering tank from the upper part, performing secondary filtering under the action of gravity, passing through 60-200 meshes of active carbon, wherein the depth is from top to bottom, the bottom layer is double-layer nylon cloth adopting a five-pointed star weaving method, ultraviolet lamps are arranged at the top and the bottom for disinfection, and finally, discharging after detection, and returning to an anaerobic reaction tank if the sewage does not reach the standard;
the sewage treatment apparatus includes: an aerobic strain tank, an anaerobic strain tank, a grid, a regulating tank, a flocculation tank, an anaerobic reaction tank, an aerobic aeration biological filter and a methane recovery tank.
2. The microbial inoculum treatment process of livestock and poultry breeding sewage as claimed in claim 1, which is characterized in that: the anaerobic bacteria is the combination of methanogen bacillus, methanogen coccus and lactobacillus.
3. The microbial inoculum treatment process of livestock and poultry breeding sewage as claimed in claim 2, which is characterized in that: the inoculation ratio of methanogen bacillus, methanogen coccus and lactobacillus is 1:1: 2.
4. The microbial inoculum treatment process of livestock and poultry breeding sewage as claimed in claim 1, which is characterized in that: the aerobic bacteria is the combination of sulfureted bacteria, phosphorus-accumulating bacteria, hydrolytic acidification bacteria and saccharomycetes.
5. The microbial inoculum treatment process of livestock and poultry breeding sewage as claimed in claim 4, which is characterized in that: the inoculation ratio of the vulcanized bacteria, the phosphorus-accumulating bacteria, the hydrolytic acidification bacteria and the yeast is 2:2:3: 5.
6. The microbial inoculum treatment process of livestock and poultry breeding sewage as claimed in claim 4, which is characterized in that: the sulfuration bacteria is one or two of thiobacillus thiooxidans, thiobacillus thioparus, thiobacillus ferrooxidans and thiobacillus denitrificans.
7. The microbial inoculum treatment process of livestock and poultry breeding sewage as claimed in claim 4, which is characterized in that: the phosphorus-accumulating bacteria is one or two of acinetobacter calcoaceticus, acinetobacter juniperi, denitrifying phosphorus-accumulating bacteria and rhodochrous bacteria.
8. The microbial inoculum treatment process of livestock and poultry breeding sewage as claimed in claim 4, which is characterized in that: the hydrolytic acidification bacteria is one or two of bacillus cereus, clostridium amyloliquefaciens and bacteroides succinogenes.
9. The microbial inoculum treatment process of livestock and poultry breeding sewage as claimed in claim 1, which is characterized in that: the stirring speed in the step (2) is 400 rpm-600 rpm; in the step (3), the shaking table speed is 100-150 r/min; in the step (5), the stirring speed is 200 rpm-400 rpm; in the step (6), the stirring speed is 300-500 rpm.
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