CN109289500A - Microbial deodorant bacterium solution and preparation method thereof - Google Patents

Microbial deodorant bacterium solution and preparation method thereof Download PDF

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Publication number
CN109289500A
CN109289500A CN201811329146.5A CN201811329146A CN109289500A CN 109289500 A CN109289500 A CN 109289500A CN 201811329146 A CN201811329146 A CN 201811329146A CN 109289500 A CN109289500 A CN 109289500A
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China
Prior art keywords
parts
lactobacillus
bacterium solution
microbial deodorant
saccharomycete
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CN201811329146.5A
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Chinese (zh)
Inventor
胡福利
徐云翔
王启佩
武韩
陈景岩
胡福超
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Shandong Zhuohua Biotechnology Co Ltd
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Shandong Zhuohua Biotechnology Co Ltd
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Priority to CN201811329146.5A priority Critical patent/CN109289500A/en
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    • BPERFORMING OPERATIONS; TRANSPORTING
    • B01PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01DSEPARATION
    • B01D53/00Separation of gases or vapours; Recovering vapours of volatile solvents from gases; Chemical or biological purification of waste gases, e.g. engine exhaust gases, smoke, fumes, flue gases, aerosols
    • B01D53/34Chemical or biological purification of waste gases
    • B01D53/74General processes for purification of waste gases; Apparatus or devices specially adapted therefor
    • B01D53/84Biological processes
    • BPERFORMING OPERATIONS; TRANSPORTING
    • B01PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01DSEPARATION
    • B01D53/00Separation of gases or vapours; Recovering vapours of volatile solvents from gases; Chemical or biological purification of waste gases, e.g. engine exhaust gases, smoke, fumes, flue gases, aerosols
    • B01D53/34Chemical or biological purification of waste gases
    • B01D53/46Removing components of defined structure
    • B01D53/48Sulfur compounds
    • B01D53/52Hydrogen sulfide
    • BPERFORMING OPERATIONS; TRANSPORTING
    • B01PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01DSEPARATION
    • B01D53/00Separation of gases or vapours; Recovering vapours of volatile solvents from gases; Chemical or biological purification of waste gases, e.g. engine exhaust gases, smoke, fumes, flue gases, aerosols
    • B01D53/34Chemical or biological purification of waste gases
    • B01D53/46Removing components of defined structure
    • B01D53/54Nitrogen compounds
    • B01D53/58Ammonia
    • BPERFORMING OPERATIONS; TRANSPORTING
    • B01PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01DSEPARATION
    • B01D53/00Separation of gases or vapours; Recovering vapours of volatile solvents from gases; Chemical or biological purification of waste gases, e.g. engine exhaust gases, smoke, fumes, flue gases, aerosols
    • B01D53/34Chemical or biological purification of waste gases
    • B01D53/46Removing components of defined structure
    • B01D53/72Organic compounds not provided for in groups B01D53/48 - B01D53/70, e.g. hydrocarbons
    • YGENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02ATECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
    • Y02A50/00TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE in human health protection, e.g. against extreme weather
    • Y02A50/20Air quality improvement or preservation, e.g. vehicle emission control or emission reduction by using catalytic converters

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  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Biomedical Technology (AREA)
  • Environmental & Geological Engineering (AREA)
  • Analytical Chemistry (AREA)
  • General Chemical & Material Sciences (AREA)
  • Oil, Petroleum & Natural Gas (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Molecular Biology (AREA)
  • Disinfection, Sterilisation Or Deodorisation Of Air (AREA)

Abstract

The invention discloses a kind of microbial deodorant bacterium solution and preparation method thereof, prepared microbial deodorant bacterium solution includes the component of following mass fraction: 2~5 parts of lactobacillus plantarum;2~5 parts of lactobacillus acidophilus;2~5 parts of lactobacillus fermenti;2~4 parts of saccharomycete.The present invention using using lactobacillus plantarum, lactobacillus acidophilus, lactobacillus fermenti and saccharomycete absorption, absorb the foul smell molecule such as hydrogen sulfide, ammonia, mercaptan, thioether be transferred in microbial cell, it decomposes and utilizes by metabolism as nutriment again, finally nitrogenous foul smell oxygenolysis, and the foul smell oxygenolysis of sulfur-bearing at S, SO3 2‑、SO4 2‑;The substance decomposition of not nitrogenous sulphur at CO2And H2Odor pollutant is converted harmless odorless substance by O etc., to achieve the purpose that improve air quality, protection human body health.

Description

Microbial deodorant bacterium solution and preparation method thereof
Technical field
The invention belongs to deodorization bacterium solution technical field, a kind of microbial deodorant bacterium solution and its preparation side are particularly related to Method.
Background technique
Odorant refers to the substance that can cause the varied smelly sense of olfactory organ, by generating source can be divided into life source and Industrial source.Life source refers to the stench generated in daily life;Industrial source refers to the stench generated in industrial production, such as cultivates Factory, dirty waste treatment plant, pharmaceutical factory, food processing factory etc..Foul gas includes: sulfur-containing compound, containing nitrogen compound, halogen And its derivative, hydro carbons, oxygen containing organic matter, these odorants, vulcanisation hydrogen and ammonia are mostly outside organic matter, through gas phase color Spectrum detection, the main ingredient of most foul gas are hydrogen sulfide and ammonia.Stench can to respiratory system, nervous system, follow Loop system, endocrine system generate strong impulse effect, and the 32nd article of the Air Pollution Prevention Law in China, 34 articles also to effluvium Strict regulations have been made in body discharge.
Mainly have to the minimizing technology of foul smell stink: chemical deodorization method, physical deodorization method, biological odor removal method.It is wherein biological Deodorization process most superiority has many advantages, such as that environmentally protective, odor removal efficient is high, wide adaptability, with strong points, at low cost, therefore Need to develop better deodorization bacterium solution.
Summary of the invention
In order to overcome drawbacks described above, the present invention provides a kind of microbial deodorant bacterium solutions and preparation method thereof, can be efficient Foul smell stink is removed, environmental and human health impacts are protected.
The present invention is to solve technical solution used by its technical problem: a kind of microbial deodorant bacterium solution, including with The component of lower mass fraction: 2~5 parts of lactobacillus plantarum;2~5 parts of lactobacillus acidophilus;2~5 parts of lactobacillus fermenti;Saccharomycete 2~ 4 parts.
The present invention also provides a kind of preparation methods of above-mentioned microbial deodorant bacterium solution, which is characterized in that including following step It is rapid:
1) by weight by 8 parts of table sugar, 7 parts of glucose, 2.5 parts of peptone, 0.3 part of magnesium sulfate, 0.1 part of tween, phosphorus 0.5 part of sour hydrogen dipotassium, 1 part of sodium acetate plus 80.5 parts of pure water dissolutions, heat treating process sterilize, and are used as culture medium;
2) 1 part of lactobacillus plantarum, 1 part of lactobacillus acidophilus, 1 part of lactobacillus fermenti, 1 part of saccharomycete are respectively connected to above-mentioned training It supports in base;
3) mixed liquor after step 2) inoculation is cultivated 48 hours at 37 DEG C, pH value is down to 3.3~3.8;
4) by the cultured products in step 3), first mixing sterile in the following proportions: 2~5 parts of lactobacillus plantarum cultured products, 2~5 parts of lactobacillus acidophilus cultured products, 2~5 parts of lactobacillus fermenti cultured products, 2~4 parts of saccharomycete cultured products;Then again It is aseptic subpackaged, microbial deodorant bacterium solution is made.
The beneficial effects of the present invention are: 1, rapid chelating, passivation foul smell molecule, quickly eliminate stink and peculiar smell;2, it eliminates The stinks such as farm, sewage plant, sludge treatment plant, Leather Factory and peculiar smell;3, atmosphere is protected, atmosphere pollution is reduced;4, it reduces empty Gas pollutes the harm to human health.
Specific embodiment
With reference to embodiments, it elaborates to several preferred embodiments of the invention.But protection scope of the present invention Be not limited to following embodiments, i.e., in every case with simple equivalence changes made by scope of the present invention patent and description and Modification, all still belongs within the invention patent covering scope.
A kind of microbial deodorant bacterium solution, the component including following mass fraction: 2~5 parts of lactobacillus plantarum;Lactobacillus acidophilus 2~5 parts;2~5 parts of lactobacillus fermenti;2~4 parts of saccharomycete;2 parts of lactic acid;2 parts of organic acid;Produce fragrant 1 part of short chain fatty acids.
The preparation method following steps of the microbial deodorant bacterium solution:
1) by weight by 8 parts of table sugar, 7 parts of glucose, 2.5 parts of peptone, 0.3 part of magnesium sulfate, 0.1 part of tween, phosphorus 0.5 part of sour hydrogen dipotassium, 1 part of sodium acetate plus 80.5 parts of pure water dissolutions, heat treating process sterilize, and are used as culture medium;
2) by 1 part of lactobacillus plantarum (Lactobacillus plantarum), lactobacillus acidophilus (Lactobacillus Acidophilus) 1 part, 1 part of lactobacillus fermenti (Lactobacillusfermentum), saccharomycete (Saccharomyces Cerevisiae it) is respectively connected in above-mentioned culture medium for 1 part;
3) mixed liquor after step 2) inoculation is cultivated 48 hours at 37 DEG C, pH value is down to 3.3~3.8;
4) by the cultured products in step 3), first mixing sterile in the following proportions: 2~5 parts of lactobacillus plantarum cultured products, 2~5 parts of lactobacillus acidophilus cultured products, 2~5 parts of lactobacillus fermenti cultured products, 2~4 parts of saccharomycete cultured products;Then again It is aseptic subpackaged, microbial deodorant bacterium solution is made.
The action principle of the microbial deodorant bacterium solution are as follows:
1, it (lactic acid, organic acid and is produced fragrant short using the metabolite of lactobacillus plantarum, lactobacillus acidophilus, lactobacillus fermenti Chain fatty acid) to foul smell composition (such as H2S、NH3, mercaptan, thioether etc.) highly effective chelating effect, lose the active group of foul smell Effect, stink disappear.
2, using the absorption of lactobacillus plantarum, lactobacillus acidophilus, lactobacillus fermenti and saccharomycete, absorb hydrogen sulfide, ammonia The foul smell molecule such as gas, mercaptan, thioether is transferred in microbial cell, then passes through metabolism point as nutriment Solution and utilization, finally nitrogenous foul smell, if amine oxygenolysis is at NH4 +、NO2 -、NO3 -;The foul smell oxygenolysis of sulfur-bearing at S、SO3 2-、SO4 2-;The substance decomposition of not nitrogenous sulphur at CO2And H2O etc..Harmless odorless substance is converted by odor pollutant, with Achieve the purpose that improve air quality, protection human body health.
1~5 the present invention will be described by the following examples.
Examples 1 to 7 is respectively according to composition of raw materials and technological parameter shown in table 1.
1. Examples 1 to 5 of table and reference example 1~2
Above-described embodiment is subjected to Deodorization Experiment:
Odor concentration uses triangle odor bag method (GB/T 14675-1993), and ammonia uses sodium hypochlorite-salicylic acid Spectrophotometry (GB/T 14679-1993), hydrogen sulfide use polyvinyl ammonium absorption-methylene-blue colorimetric method, and total hydrocarbon is adopted With gas chromatography (GB/T 15263-1994).
1, the laboratory deodorizing effect experiment of microbial deodorant bacterium solution
Deodorizing effect after ten minutes is sprayed using smelly feces of livestock and poultry of the microbial deodorant bacterium solution to confined space.It is real The efficacy in laboratory of example 1~7 is applied, is shown in Table 2~table 8 respectively.
The laboratory deodorizing effect of 2. embodiment 1 of table is tested
The laboratory deodorizing effect of 3. embodiment 2 of table is tested
The laboratory deodorizing effect of 4. embodiment 3 of table is tested
The laboratory deodorizing effect of 5. embodiment 4 of table is tested
The laboratory deodorizing effect of 6. embodiment 5 of table is tested
The laboratory deodorizing effect of 7. embodiment 6 of table is tested
The laboratory deodorizing effect of 8. embodiment 7 of table is tested
2, the live deodorizing effect experiment of microbial deodorant bacterium solution
It is removed respectively at eight hilllock sludge treatment plant scene of sewage purification Co., Ltd of Zhengzhou City with embodiment 6 and embodiment 7 Smelly effect experiment, 6 deodorizing effect of embodiment are shown in Table 9, and 7 deodorizing effect of embodiment is shown in Table 10.
The live deodorizing effect of 9. embodiment of table 6
The live deodorizing effect of 10. embodiment of table 7
3, conclusion is realized:
In laboratory stage, Examples 1 to 7 is to NH3Highest removal rate be 76.9%, to H2The highest removal rate that S is gone is 83.3%.In the eight hilllock sludge treatment plants live stage, 6~7 couples of NH of embodiment3、H2S, total hydrocarbon, odor concentration have good go Except effect.
The experiment of the experiment of laboratory stage and live stage, absolutely proves the microbial deodorant bacterium solution of this formula to foul smell Removal rate in terms of, significant effect.
It can be seen that the effect of the microbial deodorant bacterium solution are as follows: 1, rapid chelating, passivation foul smell molecule are quickly eliminated smelly Taste and peculiar smell;2, stinks and the peculiar smell such as farm, sewage plant, sludge treatment plant, Leather Factory are eliminated;3, atmosphere is protected, is reduced big Gas pollution;4, the harm of air pollution on human health is reduced.

Claims (2)

1. a kind of microbial deodorant bacterium solution, which is characterized in that the component including following mass fraction: 2~5 parts of lactobacillus plantarum; 2~5 parts of lactobacillus acidophilus;2~5 parts of lactobacillus fermenti;2~4 parts of saccharomycete.
2. a kind of preparation method of microbial deodorant bacterium solution described in claim 1, which comprises the following steps:
1) by weight by 8 parts of table sugar, 7 parts of glucose, 2.5 parts of peptone, 0.3 part of magnesium sulfate, 0.1 part of tween, phosphoric acid hydrogen 0.5 part of dipotassium, 1 part of sodium acetate plus 80.5 parts of pure water dissolutions, heat treating process sterilize, and are used as culture medium;
2) 1 part of lactobacillus plantarum, 1 part of lactobacillus acidophilus, 1 part of lactobacillus fermenti, 1 part of saccharomycete are respectively connected to above-mentioned culture medium It is interior;
3) mixed liquor after step 2) inoculation is cultivated 48 hours at 37 DEG C, pH value is down to 3.3~3.8;
4) by the cultured products in step 3), first mixing sterile in the following proportions: 2~5 parts of lactobacillus plantarum cultured products, acidophilus 2~5 parts of lactobacillus cultured products, 2~5 parts of lactobacillus fermenti cultured products, 2~4 parts of saccharomycete cultured products;Then sterile again Microbial deodorant bacterium solution is made in packing.
CN201811329146.5A 2018-11-09 2018-11-09 Microbial deodorant bacterium solution and preparation method thereof Pending CN109289500A (en)

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Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
KR20130105187A (en) * 2012-03-16 2013-09-25 일동제약주식회사 Method of preparing functional fermented turmeric gel using a probiotic lactic acid bacteria and functional fermented turmeric gel-il101 prepared thereby
CN103865847A (en) * 2014-03-06 2014-06-18 深圳市三林生物科技工程有限公司 Composite microbial preparation for garbage deodorization and preparation method thereof
CN107653197A (en) * 2017-11-13 2018-02-02 深圳市长隆科技有限公司 A kind of preparation method of microorganism formulation and its application in leather waste water processing
CN108048344A (en) * 2017-11-20 2018-05-18 广东博沃特生物科技有限公司 Two plants of deodorization bacterial strains and its application in composite biological deodorant is prepared
CN108456650A (en) * 2018-03-30 2018-08-28 上海华浩原益生物科技有限公司 A kind of complex microorganism deodorizing microorganism and its preparation method and application

Patent Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
KR20130105187A (en) * 2012-03-16 2013-09-25 일동제약주식회사 Method of preparing functional fermented turmeric gel using a probiotic lactic acid bacteria and functional fermented turmeric gel-il101 prepared thereby
CN103865847A (en) * 2014-03-06 2014-06-18 深圳市三林生物科技工程有限公司 Composite microbial preparation for garbage deodorization and preparation method thereof
CN107653197A (en) * 2017-11-13 2018-02-02 深圳市长隆科技有限公司 A kind of preparation method of microorganism formulation and its application in leather waste water processing
CN108048344A (en) * 2017-11-20 2018-05-18 广东博沃特生物科技有限公司 Two plants of deodorization bacterial strains and its application in composite biological deodorant is prepared
CN108456650A (en) * 2018-03-30 2018-08-28 上海华浩原益生物科技有限公司 A kind of complex microorganism deodorizing microorganism and its preparation method and application

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Application publication date: 20190201