CN102391966A - Composite bacteria for treating food waste percolate and preparation method thereof - Google Patents
Composite bacteria for treating food waste percolate and preparation method thereof Download PDFInfo
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Abstract
The invention discloses composite bacteria for treating food waste percolate and a preparation method thereof, and the preparation method comprises the following steps: respectively performing high-density culture on strains of trichoderma viride, bacillus subtilis, bread mold, photosynthetic bacteria, saccharomyces cerevisiae, bacillus licheniformis, nitrosomonas europaea and nitrobacterwinogradskyi, performing dehydration and drying on various types of monoxenie obtained by high-density culture to prepare hypopus microbial dry powder, and then mixing according to the weight ratio of 5-15 parts of the trichoderma viride, 10-15 parts of the bacillus subtilis, 20-30 parts of the bread mold, 10-15 parts of the photosynthetic bacteria, 7-15 parts of the saccharomyces cerevisiae, 10-15 parts of the bacillus licheniformis, 10-20 parts of the nitrosomonas europaea and the 10-20 parts of the nitrobacterwinogradskyi to prepare the composite bacteria for treating the food waste percolate. By using the composite bacteria for treating the waste percolate, the use is convenient, the effects of fermentation and degradation are good and the production cost is low.
Description
Technical field
The present invention relates to biotechnology microbial fermentation technology field, relate in particular to a kind of composite bacteria that is used for the processing utensil rubbishes percolate and preparation method thereof.
Background technology
The changing food waste water ratio can reach more than the 90wt%, therefore in stacking process, very easily produces a large amount of percolates, and the changing food waste percolate has following characteristics: 1, water quality is complicated, and hazardness is big.There are some researches show that utilization GC-MS coupling technique is analyzed organic pollutant composition in the percolate, detects altogether and mainly contains 63 kinds of organic pollutants in the percolate, confidence level is at have 34 kinds more than 60%.Wherein, 6 kinds in alkane alkene, 19 kinds of carboxylic-acids, 5 kinds of ester classes, 10 kinds of alcohol, phenols, 10 kinds of aldehyde, ketones, 7 kinds of amidess, a kind of aromatic hydrocarbons, other 5 kinds.Wherein be confirmed to be carcinogenic a kind, 4 kinds of short cancer thing, epicarcinogens, a kind of mutagen is put into have 6 kinds of China's environment priority pollutant " Black List ".2, CODcr and BOD5 concentration are high.CODcr and BOD5 best result Bie Keda 90000 mg/L, 38000mg/L even higher in the percolate.3, ammonia-nitrogen content is high, and raises with the prolongation of landfill time, reaches as high as 1700mg/L.4, the microbial nutrition element ratio in percolate imbalance mainly is the out of proportion of C, N, P.
Rubbish leachate treatment method comprises physico-chemical processes and biological process.Physico-chemical processes mainly contains charcoal absorption, chemical precipitation, density separation, chemical oxidation, chemical reduction, IX, film dialysis, gas and mentions several different methods such as wet oxidation process; When COD is 2000～4000mg/L; The COD clearance of physico-chemical process can reach 50%～87%; But the physico-chemical process processing cost is higher, is inappropriate for the processing of big yield percolate, and therefore percolate mainly is to adopt biological process at present.Biological process is divided into aerobe processing, anaerobic biological treatment and the combination of the two.Aerobic treatment comprises activated sludge process, aeration oxidation pond, aerobic stabilization pond, blodisc and trickling filter etc.Anaerobic treatment comprises upward flow Sludge Bed, anaerobic immobilized bio-reactor, mixing reactor and anaerobism stabilization pond; But, can in biological degradation, not play a role so form the microniological proudcts of industrialization so far because COD, metals ion, ammonia nitrogen equal size are high in the percolate.
Summary of the invention
First technical problem to be solved by this invention is: to the deficiency that prior art exists, a kind of effectively biodegrade, the composite bacteria that is used for the processing utensil rubbishes percolate easy to use are provided.
Second technical problem to be solved by this invention is: to the deficiency that prior art exists, the preparation method of a kind of effectively biodegrade, the composite bacteria that is used for the processing utensil rubbishes percolate easy to use is provided.
For solving above-mentioned first technical problem, technical scheme of the present invention is:
A kind of composite bacteria that is used for the processing utensil rubbishes percolate, mainly the raw materials mix preparation by following weight part forms:
5～15 parts of virides (Trichoderma viride),
10～15 parts of subtilises (Bacillus subtilis),
20～30 parts of bread moulds (Black Rhizopus),
10～15 parts of photosynthetic bacteriums (photosynthetic bacteria),
7～15 parts of yeast saccharomyces cerevisiaes (Saccharomyces cerevisiae),
10～15 parts of Bacillus licheniformis (Bacillus licheniformis),
10～20 parts of nitrosomonus (Nitrosomonas europaea),
10～20 parts of Vickers Nitrate bacterias (Nitrobacter winogradskyi).
For solving above-mentioned second technical problem, technical scheme of the present invention is:
A kind of preparation method who is used for the composite bacteria of processing utensil rubbishes percolate may further comprise the steps:
Bacterial classification with viride, subtilis, bread mould, photosynthetic bacterium, yeast saccharomyces cerevisiae, Bacillus licheniformis, nitrosomonus and Vickers Nitrate bacteria carries out high-density culture respectively; At first be seeded to the activation culture in the bottle of shaking that volume ratio is 15～30% substratum is housed; With the cultured bacterial classification inoculation enlarged culturing of fermenting in the fermentor tank; Various single bacterium that enlarged culturing is obtained dehydrates; Be prepared into the hypopus microbial dry powder; According to the weight ratio of 10～20 parts of 5～15 parts of virides, 10～15 parts of subtilises, 20～30 parts of bread moulds, 10～15 parts of photosynthetic bacteriums, 7～15 parts of yeast saccharomyces cerevisiaes, 10～15 parts of Bacillus licheniformis, 10～20 parts of nitrosomonus and Vickers Nitrate bacterias, mix the composite bacteria that obtains being used for the processing utensil rubbishes percolate then.
Wherein, the preparation of said viride hypopus microbial dry powder may further comprise the steps:
Viride bacterial classification on the slant medium that purifying is cultivated is seeded to volume ratio is housed is shaking in the bottle of 15～30% activation medium; Said activation medium is a wort potato sucrose substratum; Be 28～30 ℃ in culture temperature, shake bottle rotating speed a 140～180rpm, activation culture 32～48h;
The viride bacterial classification that activation culture is good is inoculated into and is equipped with in the fermentor tank that volume ratio is 50～70% fermention mediums; Inoculum size is a volume ratio 1～3%; The prescription of said fermention medium is glucose 8～12g/l, corn cob 5～6g/l, calcium chloride 0.2～0.6g/l, sal epsom 0.1～0.5g/l, potassium primary phosphate 18～22g/l, yeast extract paste 8～12g/l, ferrous sulfate 3～7mg/l, zinc sulfate 1.0～1.8mg/l, NSC 51149 3.5～4.0mg/l and manganous sulfate 1.2～1.8mg/l; In culture temperature is 28～30 ℃; Fermentor tank mixing speed 140～180rpm, enlarged culturing 68～84h, viride content>=10 to the fermentor tank
8Individual/ml, the viride list bacterium that then fermentation is obtained is prepared into the hypopus microbial dry powder through lyophilize.
The preparation of the hypopus microbial dry powder of said subtilis may further comprise the steps:
Bacillus subtilis strain on the slant medium that purifying is cultivated is seeded to volume ratio is housed is shaking in the bottle of 15～30% activation medium; The prescription of said activation medium is glucose 18～22g/l, peptone 12～18g/l, sodium-chlor 3～7g/l and Carnis Bovis seu Bubali cream 0.2～0.8g/l; In culture temperature is 36～40 ℃, shakes bottle rotating speed 180～220rpm and cultivates 20～32h;
The Bacillus subtilis strain that activation culture is good is inoculated into and is equipped with in the fermentor tank that volume ratio is 50～70% fermention mediums; The volume inoculum size is 1～3%; The prescription of said fermention medium is Semen Maydis powder 10～15g/l, glucose 3～8g/l, soybean cake powder 18～22g/l, fish meal 3～8g/l, lime carbonate 5～9g/l, ammonium sulfate 0.8～1.2g/l, potassium hydrogenphosphate 0.1～0.5g/l, sal epsom 0.1～0.3g/l and manganous sulfate 0.1～0.3g/l; In culture temperature is 36～40 ℃; Fermentor tank mixing speed 200～220rpm cultivates 20～32h, bacillus subtilis bacterial content>=10 in the fermentor tank
9Individual/ml, the single bacterium of subtilis that then fermentation is obtained is prepared into the hypopus microbial dry powder through drying.
The preparation of the hypopus microbial dry powder of said bread mould may further comprise the steps:
Bread mould bacterial classification on the slant medium that purifying is cultivated is seeded to volume ratio is housed is shaking in the bottle of 15～30% activation medium; The prescription of said activation medium is potato juice 18～22g/l, glucose 1～5g/l, potassium hydrogenphosphate 1～5g/l, sal epsom 1～2g/l, the VitB1 0.01～0.05g/l of 20wt%; In culture temperature is 28～30 ℃, shakes bottle rotating speed 140～180rpm and cultivates 32～42h;
The bread mould bacterial classification that activation culture is good is inoculated into and is equipped with in the fermentor tank that volume ratio is 50～70% fermention mediums; The volume inoculum size is 1～3%; The prescription of said fermention medium is steeping water 25～35g/l, sucrose 20～40g/l, urea 4～8g/l, potassium hydrogenphosphate 0.8～1.2g/l, sal epsom 0.3～0.8g/l, Repone K 0.3～0.8g/l and sweet oil 8～12g/l; In culture temperature is 28～30 ℃; Fermentor tank mixing speed 140～180rpm cultivates 42～54h, bread mould content>=10 in the fermentor tank
8Individual/ml, the bread mould list bacterium that then fermentation is obtained is prepared into the hypopus microbial dry powder through lyophilize.
The preparation of the hypopus microbial dry powder of said photosynthetic bacterium may further comprise the steps:
Photosynthetic bacterium bacterial classification inoculation on the slant medium that purifying is cultivated is shaking in the bottle of 15～30% substratum to volume ratio is housed, and is 28～30 ℃ in culture temperature, shakes that bottle is every to stir once cultivation 64～84h at a distance from 24 hours; The photosynthetic bacterium bacterial classification inoculation that activation culture is good is to being equipped with in the fermentor tank that volume ratio is 50～70% substratum; The volume inoculum size is 6～10%; In culture temperature was 28～30 ℃, whenever stirred half a hour, mixing speed 140～180rpm at a distance from 24 hours; Cultivate 100～150h, photosynthetic bacterium content>=10 to the fermentor tank
8Individual/ml; The single bacterium of photosynthetic bacterium that then fermentation is obtained is prepared into the hypopus microbial dry powder through lyophilize, and the prescription of the substratum when said activation culture and fermentation is ammonium chloride 1～3g/l, potassium hydrogenphosphate 0.1～0.3g/l, sodium acetate 2～6g/l, sodium hydrogencarbonate 1～3g/l, sodium-chlor 0.5～1.5g/l, yeast extract paste 0.1～0.2g/l and sal epsom 0.1～0.3g/l.
The preparation of said yeast saccharomyces cerevisiae hypopus microbial dry powder may further comprise the steps:
Yeast saccharomyces cerevisiae bacterial classification on the slant medium that purifying is cultivated is seeded to volume ratio is housed is shaking in the bottle of 15～30% substratum, is 28～30 ℃ in culture temperature, shakes bottle rotating speed a 140～180rpm, activation culture 32～48h; The yeast saccharomyces cerevisiae bacterial classification that activation culture is good is inoculated into and is equipped with in the fermentor tank that volume ratio is 50～70% substratum; Inoculum size is a volume ratio 1～3%; Substratum when said activation culture and fermentation is a wort potato sucrose substratum, is 28～30 ℃ in culture temperature, fermentor tank mixing speed 140～180rpm; Enlarged culturing 68～84h, yeast saccharomyces cerevisiae content>=10 to the fermentor tank
9Individual/ml, the yeast saccharomyces cerevisiae list bacterium that then fermentation is obtained is prepared into the hypopus microbial dry powder through lyophilize.
The preparation of the hypopus microbial dry powder of said Bacillus licheniformis may further comprise the steps:
Bacillus licheniformis bacterial classification inoculation on the slant medium that purifying is cultivated is shaking in the bottle of 15～30% activation medium to volume ratio is housed; The prescription of said activation medium is peptone 8～12g/l, Carnis Bovis seu Bubali cream 1～5g/l and sodium-chlor 3～7g/l; In culture temperature is 36～40 ℃, shakes bottle rotating speed 180～220rpm and cultivates 20～32h;
The Bacillus licheniformis bacterial classification inoculation that activation culture is good is to being equipped with in the fermentor tank that volume ratio is 50～70% fermention mediums; The volume inoculum size is 1～3%; The prescription of said fermention medium is soybean cake powder 13～17g/l, Semen Maydis powder 13～17g/l, potassium hydrogenphosphate 0.05～0.15g/l, potassium primary phosphate 0.05～0.15g/l and calcium chloride 2～6g/l; In culture temperature is 36～40 ℃; Fermentor tank mixing speed 200～220rpm cultivates 20～32h, Bacillus licheniformis content>=10 in the fermentor tank
9Individual/ml, the single bacterium of Bacillus licheniformis that then fermentation is obtained is prepared into the hypopus microbial dry powder through drying.
The preparation of the hypopus microbial dry powder of said nitrosomonus may further comprise the steps:
Nitrosomonus bacterial classification inoculation on the slant medium that purifying is cultivated is shaking in the bottle of 15～30% activation medium to volume ratio is housed, and is 24～28 ℃ in culture temperature, shakes bottle rotating speed 120～140rpm and cultivates a 90～120h; The nitrosomonus bacterial classification inoculation that activation culture is good is to being equipped with in the fermentor tank that volume ratio is 50～70% fermention mediums; The volume inoculum size is 1～3%; In culture temperature is 24～28 ℃; Fermentor tank mixing speed 120～140rpm cultivates 150～180h, nitrosomonus content>=10 in the fermentor tank
8Individual/ml; The single bacterium of nitrosomonus that then fermentation is obtained is prepared into the hypopus microbial dry powder through lyophilize, and the prescription of the substratum when said activation culture and fermentation is ammonium sulfate 1～3g/l, SODIUM PHOSPHATE, MONOBASIC 0.2～0.3g/l, manganous sulfate 0.005～0.015g/l, potassium hydrogenphosphate 0.5～1.0g/l, sal epsom 0.02～0.04g/l and lime carbonate 3～7g/l.
The preparation of the hypopus microbial dry powder of said Vickers Nitrate bacteria may further comprise the steps:
Vickers Nitrate bacteria bacterial classification inoculation on the slant medium that purifying is cultivated is shaking in the bottle of 15～30% activation medium to volume ratio is housed, and is 24～28 ℃ in culture temperature, shakes bottle rotating speed 120～140rpm and cultivates a 68～78h; The Vickers Nitrate bacteria bacterial classification inoculation that activation culture is good is to being equipped with in the fermentor tank that volume ratio is 50～70% fermention mediums; The volume inoculum size is 1～3%; In culture temperature is 24～28 ℃; Fermentor tank mixing speed 120～140rpm cultivates 180～220h, Vickers Nitrate bacteria content>=10 in the fermentor tank
8Individual/ml; The single bacterium of Vickers Nitrate bacteria that then fermentation is obtained is prepared into the hypopus microbial dry powder through lyophilize, and the prescription of the substratum when said activation culture and fermentation is Sodium Nitrite 0.5～1.5g/l, sal epsom 0.01～0.05g/l, manganous sulfate 0.005～0.015g/l, potassium hydrogenphosphate 0.5～1.0g/l, yellow soda ash 0.5～1.5g/l, SODIUM PHOSPHATE, MONOBASIC 0.2～0.3g/l and lime carbonate 0.5～1.5g/l.
Viride among the present invention can produce the enzyme system of multiple biologically active, as: cellulase, chitinase, zytase etc.Viride is one of the highest bacterial strain of institute's cellulase-producing activity; The cellulase that is produced has Degradation to crop; Effect is very good, is again a kind of resourceful antagonistic microbe, in the plant pathology biological control, has important effect; Have protection and treatment double effects, can effectively prevent and treat soil-borne disease.
Subtilis among the present invention is the important production bacterium of AMS and neutral protease; The subtilyne that produces in the subtilis thalli growth process, polymyxin, nystatin, linear gramicidins isoreactivity material have the obvious suppression effect to the conditioned pathogen of pathogenic bacterium or autogenous infection; Subtilis thalline self synthesizes enzymes such as AMS, proteolytic enzyme, lypase, cellulase; Subtilis has very strong restraining effect to unwanted bacterias such as vibrios, intestinal bacteria and baculoviruss; Secrete the function of a large amount of chitinases; Chitinase can decompose the cell walls of pathogenic fungi and suppress fungal disease, decompose hazardous and noxious substances, purify water, residual bait, ight soil, organism etc. in the decomposing pool, have the short grained effect of rubbish in the very strong cleaning water.
Bread mould among the present invention is very wide in distributed in nature, and is of many uses, and its amylase activity is very strong, is amylomyces commonly used in the brewery industry, and has extremely strong steatolysis function.
Photosynthetic bacterium among the present invention is to carry out photosynthetic mikrobe as the energy, the organism that can under anaerobism illumination or aerobic dark condition, utilize occurring in nature, sulfide, ammonia etc. as the hydrogen donor carbon source of holding concurrently with light.Common every milliliter contains more or less a hundred PSB bacterium in, the seawater light at nature; The thalline of photosynthetic bacterium with organism such as organic acid, amino acid, ammonia and carbohydrate and hydrogen sulfide as oxygen donator; Obtain energy through photophosphorylation; In water, can directly utilize degraded organic matter and hydrogen sulfide under the illumination condition and make self to be able to propagation, with advancing to have purified water body.
Yeast saccharomyces cerevisiae among the present invention can decompose the carbohydrate that utilizes in the environment, hydrogen sulfide, ammonia etc.; Produce acidic substance in the metabolic engineering; Can effectively suppress pathogenic bacterium growths, and the yeast thalline can be used as the nutritive substance that other cooperate bacterium, help the fast breeding of composite flora.
Bacillus licheniformis among the present invention can produce the activity resistent material; And have unique biological and take the oxygen mechanism of action by force; The growth and breeding that can suppress pathogenic bacterium; Can decompose the hazardous and noxious substances in the environment, have stronger proteolytic enzyme, lypase, diastatic activity, can decompose starch, protein and lipid in the environment.
Nitrosomonus among the present invention and Vickers Nitrate bacteria, these two types of bacterium live in together usually, have avoided the accumulation of nitrite in soil, help the body normal growth.Ammonia in the soil or ammonium salt must just can change nitrate salt under the acting in conjunction of above two bacterioids, thereby increase the available nitrogen nutrition of plant.
Owing to adopted technique scheme, the invention has the beneficial effects as follows:
The present invention carries out the bacterial classification of viride, subtilis, bread mould, photosynthetic bacterium, yeast saccharomyces cerevisiae, Bacillus licheniformis, nitrosomonus and Vickers Nitrate bacteria the hypopus microbial dry powder that is prepared into after the high-density culture; Adopt rational proportion according to its purposes; Mix the processing that the composite bacteria that obtains is used for percolate; Have the following advantages: rational proportion is adopted to the staple of changing food waste percolate in (1); Composite bacteria contains corresponding various single bacterium, and the objectionable constituent in the percolate of can degrading rapidly have unusual effect to reducing ammonia nitrogen, COD, BOD etc.; (2) since organism by rapidly degraded, deodorizing effect is remarkable, uses that the stink of percolate significantly alleviates after 1 day; (3) reduce changing food waste percolate water colour; (4) use changing food waste percolate after the composite bacteria fermentative processing can be discharged into the sewage-farm and handle together, reduced cost of sewage disposal, alleviated the load of sewage work with common sewage; (5) the multiple thalline in the present invention's prescription all has the fixedly effect of heavy metal, can effectively avoid heavy metal contamination soil resource and water resources in the percolate.The present invention is used for the processing of percolate, and is easy to use, and fermentative degradation is effective, and production cost is low.
Embodiment
Below in conjunction with concrete embodiment, further set forth the present invention.
Embodiment 1
Bacterial classification to viride, subtilis, bread mould, photosynthetic bacterium, yeast saccharomyces cerevisiae, Bacillus licheniformis, nitrosomonus and Vickers Nitrate bacteria carries out high-density culture respectively:
(1) viride: the viride bacterial classification on the slant medium that purifying is cultivated is seeded to volume ratio is housed is shaking in the bottle of 15% activation medium; Said activation medium is a wort potato sucrose substratum; Be 30 ℃ in culture temperature, shake a bottle rotating speed 180rpm, activation culture 32h; The viride bacterial classification that activation culture is good is inoculated into and is equipped with in the fermentor tank that volume ratio is 50% fermention medium; Inoculum size is a volume ratio 1%; The prescription of said fermention medium is glucose 8g/l, corn cob 5g/l, calcium chloride 0.2g/l, sal epsom 0.1g/l, potassium primary phosphate 18g/l, yeast extract paste 8g/l, ferrous sulfate 3mg/l, zinc sulfate 1.0mg/l, NSC 51149 3.5mg/l and manganous sulfate 1.2mg/l; In culture temperature is 30 ℃; Fermentor tank mixing speed 140rpm, enlarged culturing 68h, viride content>=10 to the fermentor tank
8Individual/ml, the viride list bacterium that then fermentation is obtained is prepared into the hypopus microbial dry powder through lyophilize.
(2) subtilis: the Bacillus subtilis strain on the slant medium that purifying is cultivated is seeded to volume ratio is housed is shaking in the bottle of 15% activation medium; The prescription of said activation medium is glucose 18g/l, peptone 12g/l, sodium-chlor 3g/l and Carnis Bovis seu Bubali cream 0.2g/l; In culture temperature is 37 ℃, shakes bottle rotating speed 180rpm and cultivates 20h; The Bacillus subtilis strain that activation culture is good is inoculated into and is equipped with in the fermentor tank that volume ratio is 50% fermention medium; The volume inoculum size is 1%; The prescription of said fermention medium is Semen Maydis powder 10g/l, glucose 3g/l, soybean cake powder 18g/l, fish meal 3g/l, lime carbonate 5g/l, ammonium sulfate 0.8g/l, potassium hydrogenphosphate 0.1g/l, sal epsom 0.1g/l and manganous sulfate 0.1g/l; In culture temperature is 37 ℃; Fermentor tank mixing speed 220rpm cultivates 20h, bacillus subtilis bacterial content>=10 in the fermentor tank
9Individual/ml, the single bacterium of subtilis that then fermentation is obtained is prepared into the hypopus microbial dry powder through drying.
(3) bread mould: the bread mould bacterial classification on the slant medium that purifying is cultivated is seeded to volume ratio is housed is shaking in the bottle of 15% activation medium; The prescription of said activation medium is potato juice 18g/l, glucose 1g/l, potassium hydrogenphosphate 1g/l, sal epsom 1g/l, the VitB1 0.01g/l of 20wt%; In culture temperature is 30 ℃, shakes bottle rotating speed 180rpm and cultivates 32h; The bread mould bacterial classification that activation culture is good is inoculated into and is equipped with in the fermentor tank that volume ratio is 50% fermention medium; The volume inoculum size is 1%; The prescription of said fermention medium is steeping water 25g/l, sucrose 20g/l, urea 4g/l, potassium hydrogenphosphate 0.8g/l, sal epsom 0.3g/l, Repone K 0.3g/l and sweet oil 8g/l; In culture temperature is 30 ℃, and fermentor tank mixing speed 140rpm cultivates 42h, bread mould content>=10 in the fermentor tank
8Individual/ml, the bread mould list bacterium that then fermentation is obtained is prepared into the hypopus microbial dry powder through lyophilize.
(4) photosynthetic bacterium: the photosynthetic bacterium bacterial classification inoculation on the slant medium that purifying is cultivated is shaking in the bottle of 15% substratum to volume ratio is housed, and is 28 ℃ in culture temperature, shakes that bottle is every to stir once cultivation 64h at a distance from 24 hours; The photosynthetic bacterium bacterial classification inoculation that activation culture is good is to being equipped with in the fermentor tank that volume ratio is 50% substratum, and the volume inoculum size is 6%, is 28 ℃ in culture temperature; Whenever stirred half a hour at a distance from 24 hours; Mixing speed 140rpm cultivates 100h, photosynthetic bacterium content>=10 to the fermentor tank
8Individual/ml; The single bacterium of photosynthetic bacterium that then fermentation is obtained is prepared into the hypopus microbial dry powder through lyophilize, and the prescription of the substratum when said activation culture and fermentation is ammonium chloride 1g/l, potassium hydrogenphosphate 0.1g/l, sodium acetate 2g/l, sodium hydrogencarbonate 1g/l, sodium-chlor 0.5g/l, yeast extract paste 0.1g/l and sal epsom 0.1g/l.
(5) yeast saccharomyces cerevisiae: the yeast saccharomyces cerevisiae bacterial classification on the slant medium that purifying is cultivated is seeded to volume ratio is housed is shaking in the bottle of 15% substratum, is 30 ℃ in culture temperature, shakes a bottle rotating speed 140rpm, activation culture 32h; The yeast saccharomyces cerevisiae bacterial classification that activation culture is good is inoculated into and is equipped with in the fermentor tank that volume ratio is 50% substratum; Inoculum size is a volume ratio 1%; Substratum when said activation culture and fermentation is a wort potato sucrose substratum, is 30 ℃ in culture temperature, fermentor tank mixing speed 140rpm; Enlarged culturing 68h, yeast saccharomyces cerevisiae content>=10 to the fermentor tank
9Individual/ml, the yeast saccharomyces cerevisiae list bacterium that then fermentation is obtained is prepared into the hypopus microbial dry powder through lyophilize.
(6) Bacillus licheniformis: the Bacillus licheniformis bacterial classification inoculation on the slant medium that purifying is cultivated is shaking in the bottle of 15% activation medium to volume ratio is housed; The prescription of said activation medium is peptone 8g/l, Carnis Bovis seu Bubali cream 1g/l and sodium-chlor 3g/l; In culture temperature is 37 ℃, shakes bottle rotating speed 180rpm and cultivates 20h; The Bacillus licheniformis bacterial classification inoculation that activation culture is good is to being equipped with in the fermentor tank that volume ratio is 50% fermention medium; The volume inoculum size is 1%; The prescription of said fermention medium is soybean cake powder 13g/l, Semen Maydis powder 13g/l, potassium hydrogenphosphate 0.05g/l, potassium primary phosphate 0.05g/l and calcium chloride 2g/l; In culture temperature is 37 ℃, and fermentor tank mixing speed 200rpm cultivates 20h, Bacillus licheniformis content>=10 in the fermentor tank
9Individual/ml, the single bacterium of Bacillus licheniformis that then fermentation is obtained is prepared into the hypopus microbial dry powder through drying.
(7) nitrosomonus: the nitrosomonus bacterial classification inoculation on the slant medium that purifying is cultivated is shaking in the bottle of 15% activation medium to volume ratio is housed, and is 28 ℃ in culture temperature, shakes bottle rotating speed 120rpm and cultivates a 90h; The nitrosomonus bacterial classification inoculation that activation culture is good is to being equipped with in the fermentor tank that volume ratio is 50% fermention medium; The volume inoculum size is 1%; In culture temperature is 28 ℃, and fermentor tank mixing speed 120rpm cultivates 150h, nitrosomonus content>=10 in the fermentor tank
8Individual/ml; The single bacterium of nitrosomonus that then fermentation is obtained is prepared into the hypopus microbial dry powder through lyophilize, and the prescription of the substratum when said activation culture and fermentation is ammonium sulfate 1g/l, SODIUM PHOSPHATE, MONOBASIC 0.2g/l, manganous sulfate 0.005g/l, potassium hydrogenphosphate 0.5g/l, sal epsom 0.02g/l and lime carbonate 3g/l.
(8) Vickers Nitrate bacteria: the Vickers Nitrate bacteria bacterial classification inoculation on the slant medium that purifying is cultivated is shaking in the bottle of 15% activation medium to volume ratio is housed, and is 28 ℃ in culture temperature, shakes bottle rotating speed 120rpm and cultivates a 78h; The Vickers Nitrate bacteria bacterial classification inoculation that activation culture is good is to being equipped with in the fermentor tank that volume ratio is 50% fermention medium; The volume inoculum size is 1%; In culture temperature is 28 ℃, and fermentor tank mixing speed 120rpm cultivates 180h, Vickers Nitrate bacteria content>=10 in the fermentor tank
8Individual/ml; The single bacterium of Vickers Nitrate bacteria that then fermentation is obtained is prepared into the hypopus microbial dry powder through lyophilize, and the prescription of the substratum when said activation culture and fermentation is Sodium Nitrite 0.5g/l, sal epsom 0.01g/l, manganous sulfate 0.005g/l, potassium hydrogenphosphate 0.5g/l, yellow soda ash 0.5g/l, SODIUM PHOSPHATE, MONOBASIC 0.2g/l and lime carbonate 0.5g/l.
The various hypopus microbial dry powders that high-density culture is obtained; According to the weight ratio of 10 parts of 5 parts of virides, 10 parts of subtilises, 20 parts of bread moulds, 10 parts of photosynthetic bacteriums, 7 parts of yeast saccharomyces cerevisiaes, 10 parts of Bacillus licheniformis, 10 parts of nitrosomonus and Vickers Nitrate bacterias, mix the composite bacteria that obtains being used for the processing utensil rubbishes percolate.
Embodiment 2
Bacterial classification to viride, subtilis, bread mould, photosynthetic bacterium, yeast saccharomyces cerevisiae, Bacillus licheniformis, nitrosomonus and Vickers Nitrate bacteria carries out high-density culture respectively:
(1) viride: the viride bacterial classification on the slant medium that purifying is cultivated is seeded to volume ratio is housed is shaking in the bottle of 30% activation medium; Said activation medium is a wort potato sucrose substratum; Be 30 ℃ in culture temperature, shake a bottle rotating speed 140rpm, activation culture 48h; The viride bacterial classification that activation culture is good is inoculated into and is equipped with in the fermentor tank that volume ratio is 70% fermention medium; Inoculum size is a volume ratio 3%; The prescription of said fermention medium is glucose 12g/l, corn cob 6g/l, calcium chloride 0.6g/l, sal epsom 0.5g/l, potassium primary phosphate 22g/l, yeast extract paste 12g/l, ferrous sulfate 7mg/l, zinc sulfate 1.8mg/l, NSC 51149 4.0mg/l and manganous sulfate 1.8mg/l; In culture temperature is 30 ℃; Fermentor tank mixing speed 180rpm, enlarged culturing 68h, viride content>=10 to the fermentor tank
8Individual/ml, the viride list bacterium that then fermentation is obtained is prepared into the hypopus microbial dry powder through lyophilize.
(2) subtilis: the Bacillus subtilis strain on the slant medium that purifying is cultivated is seeded to volume ratio is housed is shaking in the bottle of 30% activation medium; The prescription of said activation medium is glucose 22g/l, peptone 18g/l, sodium-chlor 7g/l and Carnis Bovis seu Bubali cream 0.8g/l; In culture temperature is 37 ℃, shakes bottle rotating speed 220rpm and cultivates 20h; The Bacillus subtilis strain that activation culture is good is inoculated into and is equipped with in the fermentor tank that volume ratio is 70% fermention medium; The volume inoculum size is 3%; The prescription of said fermention medium is Semen Maydis powder 15g/l, glucose 8g/l, soybean cake powder 22g/l, fish meal 8g/l, lime carbonate 9g/l, ammonium sulfate 1.2g/l, potassium hydrogenphosphate 0.5g/l, sal epsom 0.3g/l and manganous sulfate 0.3g/l; In culture temperature is 37 ℃; Fermentor tank mixing speed 220rpm cultivates 32h, bacillus subtilis bacterial content>=10 in the fermentor tank
9Individual/ml, the single bacterium of subtilis that then fermentation is obtained is prepared into the hypopus microbial dry powder through drying.
(3) bread mould: the bread mould bacterial classification on the slant medium that purifying is cultivated is seeded to volume ratio is housed is shaking in the bottle of 30% activation medium; The prescription of said activation medium is potato juice 22g/l, glucose 5g/l, potassium hydrogenphosphate 5g/l, sal epsom 2g/l, the VitB1 0.05g/l of 20wt%; In culture temperature is 30 ℃, shakes bottle rotating speed 180rpm and cultivates 42h; The bread mould bacterial classification that activation culture is good is inoculated into and is equipped with in the fermentor tank that volume ratio is 70% fermention medium; The volume inoculum size is 3%; The prescription of said fermention medium is steeping water 35g/l, sucrose 20g/l, urea 8g/l, potassium hydrogenphosphate 1.2g/l, sal epsom 0.8g/l, Repone K 0.8g/l and sweet oil 12g/l; In culture temperature is 30 ℃, and fermentor tank mixing speed 180rpm cultivates 54h, bread mould content>=10 in the fermentor tank
8Individual/ml, the bread mould list bacterium that then fermentation is obtained is prepared into the hypopus microbial dry powder through lyophilize.
(4) photosynthetic bacterium: the photosynthetic bacterium bacterial classification inoculation on the slant medium that purifying is cultivated is shaking in the bottle of 30% substratum to volume ratio is housed, and is 30 ℃ in culture temperature, shakes that bottle is every to stir once cultivation 84h at a distance from 24 hours; The photosynthetic bacterium bacterial classification inoculation that activation culture is good is to being equipped with in the fermentor tank that volume ratio is 70% substratum, and the volume inoculum size is 10%, is 30 ℃ in culture temperature; Whenever stirred half a hour at a distance from 24 hours; Mixing speed 180rpm cultivates 150h, photosynthetic bacterium content>=10 to the fermentor tank
8Individual/ml; The single bacterium of photosynthetic bacterium that then fermentation is obtained is prepared into the hypopus microbial dry powder through lyophilize, and the prescription of the substratum when said activation culture and fermentation is ammonium chloride 3g/l, potassium hydrogenphosphate 0.3g/l, sodium acetate 6g/l, sodium hydrogencarbonate 3g/l, sodium-chlor 1.5g/l, yeast extract paste 0.2g/l and sal epsom 0.3g/l.
(5) yeast saccharomyces cerevisiae: the yeast saccharomyces cerevisiae bacterial classification on the slant medium that purifying is cultivated is seeded to volume ratio is housed is shaking in the bottle of 30% substratum, is 30 ℃ in culture temperature, shakes a bottle rotating speed 180rpm, activation culture 48h; The yeast saccharomyces cerevisiae bacterial classification that activation culture is good is inoculated into and is equipped with in the fermentor tank that volume ratio is 70% substratum; Inoculum size is a volume ratio 3%; Substratum when said activation culture and fermentation is a wort potato sucrose substratum, is 30 ℃ in culture temperature, fermentor tank mixing speed 180rpm; Enlarged culturing 84h, yeast saccharomyces cerevisiae content>=10 to the fermentor tank
9Individual/ml, the yeast saccharomyces cerevisiae list bacterium that then fermentation is obtained is prepared into the hypopus microbial dry powder through lyophilize.
(6) Bacillus licheniformis: the Bacillus licheniformis bacterial classification inoculation on the slant medium that purifying is cultivated is shaking in the bottle of 30% activation medium to volume ratio is housed; The prescription of said activation medium is peptone 12g/l, Carnis Bovis seu Bubali cream 5g/l and sodium-chlor 7g/l; In culture temperature is 37 ℃, shakes bottle rotating speed 220rpm and cultivates 32h; The Bacillus licheniformis bacterial classification inoculation that activation culture is good is to being equipped with in the fermentor tank that volume ratio is 70% fermention medium; The volume inoculum size is 3%; The prescription of said fermention medium is soybean cake powder 17g/l, Semen Maydis powder 17g/l, potassium hydrogenphosphate 0.15g/l, potassium primary phosphate 0.15g/l and calcium chloride 6g/l; In culture temperature is 37 ℃, and fermentor tank mixing speed 220rpm cultivates 32h, Bacillus licheniformis content>=10 in the fermentor tank
9Individual/ml, the single bacterium of Bacillus licheniformis that then fermentation is obtained is prepared into the hypopus microbial dry powder through drying.
(7) nitrosomonus: the nitrosomonus bacterial classification inoculation on the slant medium that purifying is cultivated is shaking in the bottle of 30% activation medium to volume ratio is housed, and is 28 ℃ in culture temperature, shakes bottle rotating speed 140rpm and cultivates a 120h; The nitrosomonus bacterial classification inoculation that activation culture is good is to being equipped with in the fermentor tank that volume ratio is 70% fermention medium; The volume inoculum size is 3%; In culture temperature is 28 ℃, and fermentor tank mixing speed 140rpm cultivates 180h, nitrosomonus content>=10 in the fermentor tank
8Individual/ml; The single bacterium of nitrosomonus that then fermentation is obtained is prepared into the hypopus microbial dry powder through lyophilize, and the prescription of the substratum when said activation culture and fermentation is ammonium sulfate 3g/l, SODIUM PHOSPHATE, MONOBASIC 0.3g/l, manganous sulfate 0.015g/l, potassium hydrogenphosphate 1.0g/l, sal epsom 0.04g/l and lime carbonate 7g/l.
(8) Vickers Nitrate bacteria: the Vickers Nitrate bacteria bacterial classification inoculation on the slant medium that purifying is cultivated is shaking in the bottle of 30% activation medium to volume ratio is housed, and is 28 ℃ in culture temperature, shakes bottle rotating speed 140rpm and cultivates a 78h; The Vickers Nitrate bacteria bacterial classification inoculation that activation culture is good is to being equipped with in the fermentor tank that volume ratio is 70% fermention medium; The volume inoculum size is 3%; In culture temperature is 28 ℃, and fermentor tank mixing speed 140rpm cultivates 220h, Vickers Nitrate bacteria content>=10 in the fermentor tank
8Individual/ml; The single bacterium of Vickers Nitrate bacteria that then fermentation is obtained is prepared into the hypopus microbial dry powder through lyophilize, and the prescription of the substratum when said activation culture and fermentation is Sodium Nitrite 1.5g/l, sal epsom 0.05g/l, manganous sulfate 0.015g/l, potassium hydrogenphosphate 1.0g/l, yellow soda ash 1.5g/l, SODIUM PHOSPHATE, MONOBASIC 0.3g/l and lime carbonate 1.5g/l.
The various hypopus microbial dry powders that high-density culture is obtained; According to the weight ratio of 20 parts of 15 parts of virides, 15 parts of subtilises, 30 parts of bread moulds, 15 parts of photosynthetic bacteriums, 15 parts of yeast saccharomyces cerevisiaes, 15 parts of Bacillus licheniformis, 20 parts of nitrosomonus and Vickers Nitrate bacterias, mix the composite bacteria that obtains being used for the processing utensil rubbishes percolate.
Embodiment 3
Bacterial classification to viride, subtilis, bread mould, photosynthetic bacterium, yeast saccharomyces cerevisiae, Bacillus licheniformis, nitrosomonus and Vickers Nitrate bacteria carries out high-density culture respectively:
(1) viride: the viride bacterial classification on the slant medium that purifying is cultivated is seeded to volume ratio is housed is shaking in the bottle of 20% activation medium; Said activation medium is a wort potato sucrose substratum; Be 30 ℃ in culture temperature, shake a bottle rotating speed 160rpm, activation culture 36h; The viride bacterial classification that activation culture is good is inoculated into and is equipped with in the fermentor tank that volume ratio is 60% fermention medium; Inoculum size is a volume ratio 2%; The prescription of said fermention medium is glucose 10g/l, corn cob 5.5g/l, calcium chloride 0.4g/l, sal epsom 0.3g/l, potassium primary phosphate 20g/l, yeast extract paste 10g/l, ferrous sulfate 5mg/l, zinc sulfate 1.5mg/l, NSC 51149 3.7mg/l and manganous sulfate 1.6mg/l; In culture temperature is 30 ℃; Fermentor tank mixing speed 160rpm, enlarged culturing 72h, viride content>=10 to the fermentor tank
8Individual/ml, the viride list bacterium that then fermentation is obtained is prepared into the hypopus microbial dry powder through lyophilize.
(2) subtilis: the Bacillus subtilis strain on the slant medium that purifying is cultivated is seeded to volume ratio is housed is shaking in the bottle of 20% activation medium; The prescription of said activation medium is glucose 20g/l, peptone 16g/l, sodium-chlor 5g/l and Carnis Bovis seu Bubali cream 0.6g/l; In culture temperature is 37 ℃, shakes bottle rotating speed 200rpm and cultivates 24h; The Bacillus subtilis strain that activation culture is good is inoculated into and is equipped with in the fermentor tank that volume ratio is 60% fermention medium; The volume inoculum size is 2%; The prescription of said fermention medium is Semen Maydis powder 12g/l, glucose 5g/l, soybean cake powder 20g/l, fish meal 5g/l, lime carbonate 7g/l, ammonium sulfate 1.0g/l, potassium hydrogenphosphate 0.3g/l, sal epsom 0.2g/l and manganous sulfate 0.2g/l; In culture temperature is 37 ℃; Fermentor tank mixing speed 210rpm cultivates 24h, bacillus subtilis bacterial content>=10 in the fermentor tank
9Individual/ml, the single bacterium of subtilis that then fermentation is obtained is prepared into the hypopus microbial dry powder through drying.
(3) bread mould: the bread mould bacterial classification on the slant medium that purifying is cultivated is seeded to volume ratio is housed is shaking in the bottle of 20% activation medium; The prescription of said activation medium is potato juice 20g/l, glucose 3g/l, potassium hydrogenphosphate 3g/l, sal epsom 1.5g/l, the VitB1 0.02g/l of 20wt%; In culture temperature is 30 ℃, shakes bottle rotating speed 160rpm and cultivates 36h; The bread mould bacterial classification that activation culture is good is inoculated into and is equipped with in the fermentor tank that volume ratio is 60% fermention medium; The volume inoculum size is 2%; The prescription of said fermention medium is steeping water 30g/l, sucrose 20g/l, urea 6g/l, potassium hydrogenphosphate 1.0g/l, sal epsom 0.5g/l, Repone K 0.5g/l and sweet oil 10g/l; In culture temperature is 30 ℃, and fermentor tank mixing speed 160rpm cultivates 48h, bread mould content>=10 in the fermentor tank
8Individual/ml, the bread mould list bacterium that then fermentation is obtained is prepared into the hypopus microbial dry powder through lyophilize.
(4) photosynthetic bacterium: the photosynthetic bacterium bacterial classification inoculation on the slant medium that purifying is cultivated is shaking in the bottle of 20% substratum to volume ratio is housed, and is 30 ℃ in culture temperature, shakes that bottle is every to stir once cultivation 72h at a distance from 24 hours; The photosynthetic bacterium bacterial classification inoculation that activation culture is good is to being equipped with in the fermentor tank that volume ratio is 60% substratum, and the volume inoculum size is 8%, is 30 ℃ in culture temperature; Whenever stirred half a hour at a distance from 24 hours; Mixing speed 160rpm cultivates 120h, photosynthetic bacterium content>=10 to the fermentor tank
8Individual/ml; The single bacterium of photosynthetic bacterium that then fermentation is obtained is prepared into the hypopus microbial dry powder through lyophilize, and the prescription of the substratum when said activation culture and fermentation is ammonium chloride 2g/l, potassium hydrogenphosphate 0.2g/l, sodium acetate 4g/l, sodium hydrogencarbonate 2g/l, sodium-chlor 1.0g/l, yeast extract paste 0.15g/l and sal epsom 0.2g/l.
(5) yeast saccharomyces cerevisiae: the yeast saccharomyces cerevisiae bacterial classification on the slant medium that purifying is cultivated is seeded to volume ratio is housed is shaking in the bottle of 20% substratum, is 30 ℃ in culture temperature, shakes a bottle rotating speed 160rpm, activation culture 38h; The yeast saccharomyces cerevisiae bacterial classification that activation culture is good is inoculated into and is equipped with in the fermentor tank that volume ratio is 60% substratum; Inoculum size is a volume ratio 2%; Substratum when said activation culture and fermentation is a wort potato sucrose substratum, is 30 ℃ in culture temperature, fermentor tank mixing speed 160rpm; Enlarged culturing 72h, yeast saccharomyces cerevisiae content>=10 to the fermentor tank
9Individual/ml, the yeast saccharomyces cerevisiae list bacterium that then fermentation is obtained is prepared into the hypopus microbial dry powder through lyophilize.
(6) Bacillus licheniformis: the Bacillus licheniformis bacterial classification inoculation on the slant medium that purifying is cultivated is shaking in the bottle of 20% activation medium to volume ratio is housed; The prescription of said activation medium is peptone 10g/l, Carnis Bovis seu Bubali cream 3g/l and sodium-chlor 5g/l; In culture temperature is 37 ℃, shakes bottle rotating speed 200rpm and cultivates 24h; The Bacillus licheniformis bacterial classification inoculation that activation culture is good is to being equipped with in the fermentor tank that volume ratio is 60% fermention medium; The volume inoculum size is 2%; The prescription of said fermention medium is soybean cake powder 15g/l, Semen Maydis powder 15g/l, potassium hydrogenphosphate 0.1g/l, potassium primary phosphate 0.1g/l and calcium chloride 4g/l; In culture temperature is 37 ℃, and fermentor tank mixing speed 210rpm cultivates 24h, Bacillus licheniformis content>=10 in the fermentor tank
9Individual/ml, the single bacterium of Bacillus licheniformis that then fermentation is obtained is prepared into the hypopus microbial dry powder through drying.
(7) nitrosomonus: the nitrosomonus bacterial classification inoculation on the slant medium that purifying is cultivated is shaking in the bottle of 20% activation medium to volume ratio is housed, and is 28 ℃ in culture temperature, shakes bottle rotating speed 130rpm and cultivates a 100h; The nitrosomonus bacterial classification inoculation that activation culture is good is to being equipped with in the fermentor tank that volume ratio is 60% fermention medium; The volume inoculum size is 2%; In culture temperature is 28 ℃, and fermentor tank mixing speed 130rpm cultivates 160h, nitrosomonus content>=10 in the fermentor tank
8Individual/ml; The single bacterium of nitrosomonus that then fermentation is obtained is prepared into the hypopus microbial dry powder through lyophilize, and the prescription of the substratum when said activation culture and fermentation is ammonium sulfate 2g/l, SODIUM PHOSPHATE, MONOBASIC 0.25g/l, manganous sulfate 0.01g/l, potassium hydrogenphosphate 0.8g/l, sal epsom 0.03g/l and lime carbonate 5g/l.
(8) Vickers Nitrate bacteria: the Vickers Nitrate bacteria bacterial classification inoculation on the slant medium that purifying is cultivated is shaking in the bottle of 20% activation medium to volume ratio is housed, and is 28 ℃ in culture temperature, shakes bottle rotating speed 130rpm and cultivates a 72h; The Vickers Nitrate bacteria bacterial classification inoculation that activation culture is good is to being equipped with in the fermentor tank that volume ratio is 60% fermention medium; The volume inoculum size is 2%; In culture temperature is 28 ℃, and fermentor tank mixing speed 130rpm cultivates 200h, Vickers Nitrate bacteria content>=10 in the fermentor tank
8Individual/ml; The single bacterium of Vickers Nitrate bacteria that then fermentation is obtained is prepared into the hypopus microbial dry powder through lyophilize, and the prescription of the substratum when said activation culture and fermentation is Sodium Nitrite 1.0g/l, sal epsom 0.03g/l, manganous sulfate 0.01g/l, potassium hydrogenphosphate 0.8g/l, yellow soda ash 1.0g/l, SODIUM PHOSPHATE, MONOBASIC 0.25g/l and lime carbonate 1.0g/l.
The various hypopus microbial dry powders that high-density culture is obtained; According to the weight ratio of 15 parts of 10 parts of virides, 12 parts of subtilises, 25 parts of bread moulds, 12 parts of photosynthetic bacteriums, 11 parts of yeast saccharomyces cerevisiaes, 12 parts of Bacillus licheniformis, 15 parts of nitrosomonus and Vickers Nitrate bacterias, mix the composite bacteria that obtains being used for the processing utensil rubbishes percolate.
Claims (10)
1. composite bacteria that is used for the processing utensil rubbishes percolate is characterized in that mainly being formed by the raw materials mix preparation of following weight part:
5～15 parts of virides,
10～15 parts of subtilises,
20～30 parts of bread moulds,
10～15 parts of photosynthetic bacteriums,
7～15 parts of yeast saccharomyces cerevisiaes,
10～15 parts of Bacillus licheniformis,
10～20 parts of nitrosomonus,
10～20 parts of Vickers Nitrate bacterias.
2. the preparation method who is used for the composite bacteria of processing utensil rubbishes percolate as claimed in claim 1 is characterized in that may further comprise the steps:
Bacterial classification with viride, subtilis, bread mould, photosynthetic bacterium, yeast saccharomyces cerevisiae, Bacillus licheniformis, nitrosomonus and Vickers Nitrate bacteria carries out high-density culture respectively; At first be seeded to the activation culture in the bottle of shaking that volume ratio is 15～30% substratum is housed; With the cultured bacterial classification inoculation enlarged culturing of fermenting in the fermentor tank; Various single bacterium that enlarged culturing is obtained dehydrates; Be prepared into the hypopus microbial dry powder; According to the weight ratio of 10～20 parts of 5～15 parts of virides, 10～15 parts of subtilises, 20～30 parts of bread moulds, 10～15 parts of photosynthetic bacteriums, 7～15 parts of yeast saccharomyces cerevisiaes, 10～15 parts of Bacillus licheniformis, 10～20 parts of nitrosomonus and Vickers Nitrate bacterias, mix the composite bacteria that obtains being used for the processing utensil rubbishes percolate then.
3. the preparation method who is used for the composite bacteria of processing utensil rubbishes percolate as claimed in claim 2 is characterized in that the preparation of said viride hypopus microbial dry powder may further comprise the steps:
Viride bacterial classification on the slant medium that purifying is cultivated is seeded to volume ratio is housed is shaking in the bottle of 15～30% activation medium; Said activation medium is a wort potato sucrose substratum; Be 28～30 ℃ in culture temperature, shake bottle rotating speed a 140～180rpm, activation culture 32～48h;
The viride bacterial classification that activation culture is good is inoculated into and is equipped with in the fermentor tank that volume ratio is 50～70% fermention mediums; Inoculum size is a volume ratio 1～3%; The prescription of said fermention medium is glucose 8～12g/l, corn cob 5～6g/l, calcium chloride 0.2～0.6g/l, sal epsom 0.1～0.5g/l, potassium primary phosphate 18～22g/l, yeast extract paste 8～12g/l, ferrous sulfate 3～7mg/l, zinc sulfate 1.0～1.8mg/l, NSC 51149 3.5～4.0mg/l and manganous sulfate 1.2～1.8mg/l; In culture temperature is 28～30 ℃; Fermentor tank mixing speed 140～180rpm, enlarged culturing 68～84h, viride content>=10 to the fermentor tank
8Individual/ml, the viride list bacterium that then fermentation is obtained is prepared into the hypopus microbial dry powder through lyophilize.
4. the preparation method who is used for the composite bacteria of processing utensil rubbishes percolate as claimed in claim 2 is characterized in that the preparation of the hypopus microbial dry powder of said subtilis may further comprise the steps:
Bacillus subtilis strain on the slant medium that purifying is cultivated is seeded to volume ratio is housed is shaking in the bottle of 15～30% activation medium; The prescription of said activation medium is glucose 18～22g/l, peptone 12～18g/l, sodium-chlor 3～7g/l and Carnis Bovis seu Bubali cream 0.2～0.8g/l; In culture temperature is 36～40 ℃, shakes bottle rotating speed 180～220rpm and cultivates 20～32h;
The Bacillus subtilis strain that activation culture is good is inoculated into and is equipped with in the fermentor tank that volume ratio is 50～70% fermention mediums; The volume inoculum size is 1～3%; The prescription of said fermention medium is Semen Maydis powder 10～15g/l, glucose 3～8g/l, soybean cake powder 18～22g/l, fish meal 3～8g/l, lime carbonate 5～9g/l, ammonium sulfate 0.8～1.2g/l, potassium hydrogenphosphate 0.1～0.5g/l, sal epsom 0.1～0.3g/l and manganous sulfate 0.1～0.3g/l; In culture temperature is 36～40 ℃; Fermentor tank mixing speed 200～220rpm cultivates 20～32h, bacillus subtilis bacterial content>=10 in the fermentor tank
9Individual/ml, the single bacterium of subtilis that then fermentation is obtained is prepared into the hypopus microbial dry powder through drying.
5. the preparation method who is used for the composite bacteria of processing utensil rubbishes percolate as claimed in claim 2 is characterized in that: the preparation of the hypopus microbial dry powder of said bread mould may further comprise the steps:
Bread mould bacterial classification on the slant medium that purifying is cultivated is seeded to volume ratio is housed is shaking in the bottle of 15～30% activation medium; The prescription of said activation medium is potato juice 18～22g/l, glucose 1～5g/l, potassium hydrogenphosphate 1～5g/l, sal epsom 1～2g/l, the VitB1 0.01～0.05g/l of 20wt%; In culture temperature is 28～30 ℃, shakes bottle rotating speed 140～180rpm and cultivates 32～42h;
The bread mould bacterial classification that activation culture is good is inoculated into and is equipped with in the fermentor tank that volume ratio is 50～70% fermention mediums; The volume inoculum size is 1～3%; The prescription of said fermention medium is steeping water 25～35g/l, sucrose 20～40g/l, urea 4～8g/l, potassium hydrogenphosphate 0.8～1.2g/l, sal epsom 0.3～0.8g/l, Repone K 0.3～0.8g/l and sweet oil 8～12g/l; In culture temperature is 28～30 ℃; Fermentor tank mixing speed 140～180rpm cultivates 42～54h, bread mould content>=10 in the fermentor tank
8Individual/ml, the bread mould list bacterium that then fermentation is obtained is prepared into the hypopus microbial dry powder through lyophilize.
6. the preparation method who is used for the composite bacteria of processing utensil rubbishes percolate as claimed in claim 2 is characterized in that: the preparation of the hypopus microbial dry powder of said photosynthetic bacterium may further comprise the steps:
Photosynthetic bacterium bacterial classification inoculation on the slant medium that purifying is cultivated is shaking in the bottle of 15～30% substratum to volume ratio is housed, and is 28～30 ℃ in culture temperature, shakes that bottle is every to stir once cultivation 64～84h at a distance from 24 hours; The photosynthetic bacterium bacterial classification inoculation that activation culture is good is to being equipped with in the fermentor tank that volume ratio is 50～70% substratum; The volume inoculum size is 6～10%; In culture temperature was 28～30 ℃, whenever stirred half a hour, mixing speed 140～180rpm at a distance from 24 hours; Cultivate 100～150h, photosynthetic bacterium content>=10 to the fermentor tank
8Individual/ml; The single bacterium of photosynthetic bacterium that then fermentation is obtained is prepared into the hypopus microbial dry powder through lyophilize, and the prescription of the substratum when said activation culture and fermentation is ammonium chloride 1～3g/l, potassium hydrogenphosphate 0.1～0.3g/l, sodium acetate 2～6g/l, sodium hydrogencarbonate 1～3g/l, sodium-chlor 0.5～1.5g/l, yeast extract paste 0.1～0.2g/l and sal epsom 0.1～0.3g/l.
7. the preparation method who is used for the composite bacteria of processing utensil rubbishes percolate as claimed in claim 2 is characterized in that the preparation of said yeast saccharomyces cerevisiae hypopus microbial dry powder may further comprise the steps:
Yeast saccharomyces cerevisiae bacterial classification on the slant medium that purifying is cultivated is seeded to volume ratio is housed is shaking in the bottle of 15～30% substratum, is 28～30 ℃ in culture temperature, shakes bottle rotating speed a 140～180rpm, activation culture 32～48h; The yeast saccharomyces cerevisiae bacterial classification that activation culture is good is inoculated into and is equipped with in the fermentor tank that volume ratio is 50～70% substratum; Inoculum size is a volume ratio 1～3%; Substratum when said activation culture and fermentation is a wort potato sucrose substratum, is 28～30 ℃ in culture temperature, fermentor tank mixing speed 140～180rpm; Enlarged culturing 68～84h, yeast saccharomyces cerevisiae content>=10 to the fermentor tank
9Individual/ml, the yeast saccharomyces cerevisiae list bacterium that then fermentation is obtained is prepared into the hypopus microbial dry powder through lyophilize.
8. the preparation method who is used for the composite bacteria of processing utensil rubbishes percolate as claimed in claim 2 is characterized in that the preparation of the hypopus microbial dry powder of said Bacillus licheniformis may further comprise the steps:
Bacillus licheniformis bacterial classification inoculation on the slant medium that purifying is cultivated is shaking in the bottle of 15～30% activation medium to volume ratio is housed; The prescription of said activation medium is peptone 8～12g/l, Carnis Bovis seu Bubali cream 1～5g/l and sodium-chlor 3～7g/l; In culture temperature is 36～40 ℃, shakes bottle rotating speed 180～220rpm and cultivates 20～32h;
The Bacillus licheniformis bacterial classification inoculation that activation culture is good is to being equipped with in the fermentor tank that volume ratio is 50～70% fermention mediums; The volume inoculum size is 1～3%; The prescription of said fermention medium is soybean cake powder 13～17g/l, Semen Maydis powder 13～17g/l, potassium hydrogenphosphate 0.05～0.15g/l, potassium primary phosphate 0.05～0.15g/l and calcium chloride 2～6g/l; In culture temperature is 36～40 ℃; Fermentor tank mixing speed 200～220rpm cultivates 20～32h, Bacillus licheniformis content>=10 in the fermentor tank
9Individual/ml, the single bacterium of Bacillus licheniformis that then fermentation is obtained is prepared into the hypopus microbial dry powder through drying.
9. the preparation method who is used for the composite bacteria of processing utensil rubbishes percolate as claimed in claim 2 is characterized in that: the preparation of the hypopus microbial dry powder of said nitrosomonus may further comprise the steps:
Nitrosomonus bacterial classification inoculation on the slant medium that purifying is cultivated is shaking in the bottle of 15～30% activation medium to volume ratio is housed, and is 24～28 ℃ in culture temperature, shakes bottle rotating speed 120～140rpm and cultivates a 90～120h; The nitrosomonus bacterial classification inoculation that activation culture is good is to being equipped with in the fermentor tank that volume ratio is 50～70% fermention mediums; The volume inoculum size is 1～3%; In culture temperature is 24～28 ℃; Fermentor tank mixing speed 120～140rpm cultivates 150～180h, nitrosomonus content>=10 in the fermentor tank
8Individual/ml; The single bacterium of nitrosomonus that then fermentation is obtained is prepared into the hypopus microbial dry powder through lyophilize, and the prescription of the substratum when said activation culture and fermentation is ammonium sulfate 1～3g/l, SODIUM PHOSPHATE, MONOBASIC 0.2～0.3g/l, manganous sulfate 0.005～0.015g/l, potassium hydrogenphosphate 0.5～1.0g/l, sal epsom 0.02～0.04g/l and lime carbonate 3～7g/l.
10. the preparation method who is used for the composite bacteria of processing utensil rubbishes percolate as claimed in claim 2 is characterized in that: the preparation of the hypopus microbial dry powder of said Vickers Nitrate bacteria may further comprise the steps:
Vickers Nitrate bacteria bacterial classification inoculation on the slant medium that purifying is cultivated is shaking in the bottle of 15～30% activation medium to volume ratio is housed, and is 24～28 ℃ in culture temperature, shakes bottle rotating speed 120～140rpm and cultivates a 68～78h; The Vickers Nitrate bacteria bacterial classification inoculation that activation culture is good is to being equipped with in the fermentor tank that volume ratio is 50～70% fermention mediums; The volume inoculum size is 1～3%; In culture temperature is 24～28 ℃; Fermentor tank mixing speed 120～140rpm cultivates 180～220h, Vickers Nitrate bacteria content>=10 in the fermentor tank
8Individual/ml; The single bacterium of Vickers Nitrate bacteria that then fermentation is obtained is prepared into the hypopus microbial dry powder through lyophilize, and the prescription of the substratum when said activation culture and fermentation is Sodium Nitrite 0.5～1.5g/l, sal epsom 0.01～0.05g/l, manganous sulfate 0.005～0.015g/l, potassium hydrogenphosphate 0.5～1.0g/l, yellow soda ash 0.5～1.5g/l, SODIUM PHOSPHATE, MONOBASIC 0.2～0.3g/l and lime carbonate 0.5～1.5g/l.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 201110365605 CN102391966B (en) | 2011-11-17 | 2011-11-17 | Composite bacteria for treating food waste percolate and preparation method thereof |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
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| CN 201110365605 CN102391966B (en) | 2011-11-17 | 2011-11-17 | Composite bacteria for treating food waste percolate and preparation method thereof |
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| CN102976801A (en) * | 2012-12-13 | 2013-03-20 | 广州舒国生物科技有限公司 | Method for producing functional microorganism organic fertilizer by using food residue |
| CN103468614A (en) * | 2013-09-16 | 2013-12-25 | 中国人民解放军总后勤部军需装备研究所 | Kitchen waste decomposition bacterial agent and preparation method thereof |
| CN104248911A (en) * | 2014-05-13 | 2014-12-31 | 江苏绿华生物工程有限公司 | Biological deodorant and preparation method thereof |
| CN104458734A (en) * | 2014-12-19 | 2015-03-25 | 中国环境科学研究院 | Method for determining reduction potential of compost and mineralized refuse |
| CN104862244A (en) * | 2015-03-26 | 2015-08-26 | 北京北华中清环境工程技术有限公司 | Efficient composite bacterial agent for removing mixed grease-containing wastewater COD, and applications thereof |
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| CN108102973A (en) * | 2018-01-31 | 2018-06-01 | 福建双环能源科技股份有限公司 | A kind of destructor plant percolate deodorization special bacterium and its application method |
| CN108772408A (en) * | 2018-06-25 | 2018-11-09 | 成都市市政工程（集团）有限责任公司 | Municipal refuse comprehensive processing technique |
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| CN102976801A (en) * | 2012-12-13 | 2013-03-20 | 广州舒国生物科技有限公司 | Method for producing functional microorganism organic fertilizer by using food residue |
| CN103468614A (en) * | 2013-09-16 | 2013-12-25 | 中国人民解放军总后勤部军需装备研究所 | Kitchen waste decomposition bacterial agent and preparation method thereof |
| CN103468614B (en) * | 2013-09-16 | 2015-05-20 | 中国人民解放军总后勤部军需装备研究所 | Kitchen waste decomposition bacterial agent and preparation method thereof |
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| CN104458734B (en) * | 2014-12-19 | 2017-01-25 | 中国环境科学研究院 | Method for determining reduction potential of compost and mineralized refuse |
| CN104458734A (en) * | 2014-12-19 | 2015-03-25 | 中国环境科学研究院 | Method for determining reduction potential of compost and mineralized refuse |
| CN104862244A (en) * | 2015-03-26 | 2015-08-26 | 北京北华中清环境工程技术有限公司 | Efficient composite bacterial agent for removing mixed grease-containing wastewater COD, and applications thereof |
| CN104862244B (en) * | 2015-03-26 | 2017-12-19 | 北京北华中清环境工程技术有限公司 | A kind of high efficiency composition microbial inoculum for removing the oil wastewater COD containing mixing and its application |
| CN105502665A (en) * | 2015-11-25 | 2016-04-20 | 广西金威生物技术有限公司 | Garbage leachate wastewater biological comprehensive treatment method |
| CN109809840A (en) * | 2016-08-28 | 2019-05-28 | 窦欣童 | A kind for the treatment of method for urban garbage |
| CN109809840B (en) * | 2016-08-28 | 2021-08-20 | 刘明 | Municipal refuse treatment method |
| CN108102973A (en) * | 2018-01-31 | 2018-06-01 | 福建双环能源科技股份有限公司 | A kind of destructor plant percolate deodorization special bacterium and its application method |
| CN108772408A (en) * | 2018-06-25 | 2018-11-09 | 成都市市政工程（集团）有限责任公司 | Municipal refuse comprehensive processing technique |
| CN109879564A (en) * | 2019-04-02 | 2019-06-14 | 成都全健水工科技有限公司 | The bioremediation of municipal sludge |
| CN109879564B (en) * | 2019-04-02 | 2023-04-25 | 四川健全环境集团有限公司 | Biological treatment method of municipal sludge |
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Address after: 261000 Shandong Weifang high tech Zone Wolong East Street and Golden Road intersection 1689 Patentee after: SHANDONG SUKAHAN BIO-TECHNOLOGY CO., LTD. Address before: 261061 Shandong Weifang high tech Zone Wolong East Street and Golden Road intersection 1689 Patentee before: Su Kehan (Weifang) Biological Engineering Co., Ltd. |