CN102391966B - Composite bacteria for treating food waste percolate and preparation method thereof - Google Patents
Composite bacteria for treating food waste percolate and preparation method thereof Download PDFInfo
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Abstract
The invention discloses composite bacteria for treating food waste percolate and a preparation method thereof, and the preparation method comprises the following steps: respectively performing high-density culture on strains of trichoderma viride, bacillus subtilis, bread mold, photosynthetic bacteria, saccharomyces cerevisiae, bacillus licheniformis, nitrosomonas europaea and nitrobacterwinogradskyi, performing dehydration and drying on various types of monoxenie obtained by high-density culture to prepare hypopus microbial dry powder, and then mixing according to the weight ratio of 5-15 parts of the trichoderma viride, 10-15 parts of the bacillus subtilis, 20-30 parts of the bread mold, 10-15 parts of the photosynthetic bacteria, 7-15 parts of the saccharomyces cerevisiae, 10-15 parts of the bacillus licheniformis, 10-20 parts of the nitrosomonas europaea and the 10-20 parts of the nitrobacterwinogradskyi to prepare the composite bacteria for treating the food waste percolate. By using the composite bacteria for treating the waste percolate, the use is convenient, the effects of fermentation and degradation are good and the production cost is low.
Description
Technical field
The present invention relates to biotechnology microbial fermentation technology field, relate in particular to a kind of composite bacteria for the treatment of food waste percolate and preparation method thereof.
Background technology
More than the changing food waste water ratio can reach 90wt%, therefore very easily produce a large amount of percolates in stacking process, food waste percolate has following characteristics: 1, water quality complexity, hazardness is large.There are some researches show, use the GC-MS coupling technique to be analyzed Components of Organic Pollutants in percolate, detect altogether in percolate and mainly contain 63 kinds of organic pollutants, confidence level is at have 34 kinds more than 60%.Wherein, 6 kinds, alkane alkene, 19 kinds of carboxylic-acids, 5 kinds of ester classes, 10 kinds of alcohol, phenols, 10 kinds of aldehyde, ketones, 7 kinds of amidess, a kind of aromatic hydrocarbons, other 5 kinds.Wherein be confirmed to be carcinogenic a kind, 4 kinds of short cancer thing, epicarcinogens, a kind of mutagen, be put into have 6 kinds of China's priority pollutant " Black List ".2, CODcr and BOD5 concentration are high.In percolate, CODcr and BOD5 best result Bie Keda 90000 mg/L, 38000mg/L are even higher.3, ammonia-nitrogen content is high, and raises with the prolongation of landfill time, reaches as high as 1700mg/L.4, the imbalance of the microbial nutrition element ratio in percolate is mainly the out of proportion of C, N, P.
The treatment process of percolate comprises physico-chemical processes and biological process.Physico-chemical processes mainly contains the several different methods such as charcoal absorption, chemical precipitation, density separation, chemical oxidation, chemical reduction, ion-exchange, film dialysis, air lift and wet oxidation process, when COD is 2000～4000mg/L, the COD clearance of physico-chemical process can reach 50%～87%, but the physico-chemical process processing cost is higher, be unsuitable for the processing of big yield percolate, therefore percolate is mainly to adopt biological process at present.Biological process is divided into aerobe processing, anaerobic biological treatment and the combination of the two.Aerobic treatment comprises activated sludge process, aeration oxidation pond, the aerobic stabilization pool, blodisc and trickling filter etc.Anaerobic treatment comprises upward flow Sludge Bed, anaerobic immobilized bio-reactor, mixing reactor and anaerobism stabilization pond, but high due to COD in percolate, metal ion, ammonia nitrogen equal size, so form so far the microniological proudcts of industrialization, can in biological degradation, not play a role.
Summary of the invention
First technical problem to be solved by this invention is: the deficiency existed for prior art provides a kind of and can effectively carry out biological degradation, the composite bacteria for the treatment of food waste percolate easy to use.
Second technical problem to be solved by this invention is: the deficiency existed for prior art provides a kind of preparation method that can effectively carry out biological degradation, the composite bacteria for the treatment of food waste percolate easy to use.
For solving above-mentioned first technical problem, technical scheme of the present invention is:
A kind of composite bacteria for the treatment of food waste percolate mainly forms by the mixed raw material of following weight part is standby:
5～15 parts of virides (Trichoderma viride),
10～15 parts of subtilises (Bacillus subtilis),
20～30 parts of bread moulds (Black Rhizopus),
10～15 parts of photosynthetic bacteriums (photosynthetic bacteria),
7～15 parts of yeast saccharomyces cerevisiaes (Saccharomyces cerevisiae),
10～15 parts of Bacillus licheniformis (Bacillus licheniformis),
10～20 parts of nitrosomonus (Nitrosomonas europaea),
10～20 parts of Vickers Nitrate bacterias (Nitrobacter winogradskyi).
For solving above-mentioned second technical problem, technical scheme of the present invention is:
A kind of preparation method of the composite bacteria for the treatment of food waste percolate comprises the following steps:
Respectively by viride, subtilis, bread mould, photosynthetic bacterium, yeast saccharomyces cerevisiae, Bacillus licheniformis, the bacterial classification of nitrosomonus and Vickers Nitrate bacteria carries out high-density culture, at first be seeded to activation culture in the shaking flask that the substratum that volume ratio is 15～30% is housed, cultured bacterial classification is inoculated in fermentor tank to the enlarged culturing of being fermented, various single bacterium that enlarged culturing is obtained dehydrates, be prepared into the hypopus microbial dry powder, then according to 5～15 parts of virides, 10～15 parts of subtilises, 20～30 parts of bread moulds, 10～15 parts of photosynthetic bacteriums, 7～15 parts of yeast saccharomyces cerevisiaes, 10～15 parts of Bacillus licheniformis, the weight ratio that 10～20 parts of nitrosomonus and Vickers Nitrate bacteria are 10～20 parts, be mixed to get the composite bacteria for the treatment of food waste percolate.
Wherein, the preparation of described viride hypopus microbial dry powder comprises the following steps:
Viride bacterial classification on the slant medium that purifying is cultivated is seeded in the shaking flask that the activation medium that volume ratio is 15～30% is housed, described activation medium is wort potato sucrose substratum, in culture temperature, be 28～30 ℃, shaking flask rotating speed 140～180rpm, activation culture 32～48h;
By activation culture, good viride bacterial classification is inoculated into and is equipped with in the fermentor tank that volume ratio is 50～70% fermention mediums, inoculum size is volume ratio 1～3%, the formula of described fermention medium is glucose 8～12g/l, corn cob 5～6g/l, calcium chloride 0.2～0.6g/l, sal epsom 0.1～0.5g/l, potassium primary phosphate 18～22g/l, yeast extract paste 8～12g/l, ferrous sulfate 3～7mg/l, zinc sulfate 1.0～1.8mg/l, cobalt chloride 3.5～4.0mg/l and manganous sulfate 1.2～1.8mg/l, in culture temperature, it is 28～30 ℃, fermentor tank mixing speed 140～180rpm, enlarged culturing 68～84h, to the mould content of fermentor tank Green wood>=10
8individual/ml, the viride list bacterium then fermentation obtained is prepared into the hypopus microbial dry powder through lyophilize.
The preparation of the hypopus microbial dry powder of described subtilis comprises the following steps:
Bacillus subtilis strain on the slant medium that purifying is cultivated is seeded in the shaking flask that the activation medium that volume ratio is 15～30% is housed, the formula of described activation medium is glucose 18～22g/l, peptone 12～18g/l, sodium-chlor 3～7g/l and extractum carnis 0.2～0.8g/l, in culture temperature, be 36～40 ℃, shaking flask rotating speed 180～220rpm cultivates 20～32h;
By activation culture, good Bacillus subtilis strain is inoculated into and is equipped with in the fermentor tank that volume ratio is 50～70% fermention mediums, the volume inoculum size is 1～3%, the formula of described fermention medium is Semen Maydis powder 10～15g/l, glucose 3～8g/l, soybean cake powder 18～22g/l, fish meal 3～8g/l, calcium carbonate 5～9g/l, ammonium sulfate 0.8～1.2g/l, dipotassium hydrogen phosphate 0.1～0.5g/l, sal epsom 0.1～0.3g/l and manganous sulfate 0.1～0.3g/l, in culture temperature, it is 36～40 ℃, fermentor tank mixing speed 200～220rpm cultivates 20～32h, bacillus subtilis bacterial content>=10 in fermentor tank
9individual/ml, the single bacterium drying of the subtilis then fermentation obtained is prepared into the hypopus microbial dry powder.
The preparation of the hypopus microbial dry powder of described bread mould comprises the following steps:
Bread mould bacterial classification on the slant medium that purifying is cultivated is seeded in the shaking flask that the activation medium that volume ratio is 15～30% is housed, potato juice 18～22g/l that the formula of described activation medium is 20wt%, glucose 1～5g/l, dipotassium hydrogen phosphate 1～5g/l, sal epsom 1～2g/l, VitB1 0.01～0.05g/l, in culture temperature, be 28～30 ℃, shaking flask rotating speed 140～180rpm cultivates 32～42h;
By activation culture, good bread mould bacterial classification is inoculated into and is equipped with in the fermentor tank that volume ratio is 50～70% fermention mediums, the volume inoculum size is 1～3%, the formula of described fermention medium is corn steep liquor 25～35g/l, sucrose 20～40g/l, urea 4～8g/l, dipotassium hydrogen phosphate 0.8～1.2g/l, sal epsom 0.3～0.8g/l, Repone K 0.3～0.8g/l and sweet oil 8～12g/l, in culture temperature, it is 28～30 ℃, fermentor tank mixing speed 140～180rpm cultivates 42～54h, bread mould content>=10 in fermentor tank
8individual/ml, the bread mould list bacterium then fermentation obtained is prepared into the hypopus microbial dry powder through lyophilize.
The preparation of the hypopus microbial dry powder of described photosynthetic bacterium comprises the following steps:
Photosynthetic bacterium bacterial classification on the slant medium that purifying is cultivated is seeded in the shaking flask that the substratum that volume ratio is 15～30% is housed, and in culture temperature, is 28～30 ℃, and shaking flask stirred once every 24 hours, cultivated 64～84h; By activation culture, good photosynthetic bacterium bacterial classification is inoculated into and is equipped with in the fermentor tank that volume ratio is 50～70% substratum, the volume inoculum size is 6～10%, in culture temperature, it is 28～30 ℃, every 24 hours, stir half an hour, mixing speed 140～180rpm, cultivate 100～150h, to photosynthetic bacterium content>=10 in fermentor tank
8individual/ml, then the single bacterium of photosynthetic bacterium fermentation obtained is prepared into the hypopus microbial dry powder through lyophilize, and the formula of substratum when described activation culture and fermentation is ammonium chloride 1～3g/l, dipotassium hydrogen phosphate 0.1～0.3g/l, sodium acetate 2～6g/l, sodium bicarbonate 1～3g/l, sodium-chlor 0.5～1.5g/l, yeast extract paste 0.1～0.2g/l and sal epsom 0.1～0.3g/l.
The preparation of described yeast saccharomyces cerevisiae hypopus microbial dry powder comprises the following steps:
Yeast saccharomyces cerevisiae bacterial classification on the slant medium that purifying is cultivated is seeded in the shaking flask that the substratum that volume ratio is 15～30% is housed, and in culture temperature, is 28～30 ℃, shaking flask rotating speed 140～180rpm, activation culture 32～48h; By activation culture, good yeast saccharomyces cerevisiae bacterial classification is inoculated into and is equipped with in the fermentor tank that volume ratio is 50～70% substratum, inoculum size is volume ratio 1～3%, substratum when described activation culture and fermentation is wort potato sucrose substratum, in culture temperature, it is 28～30 ℃, fermentor tank mixing speed 140～180rpm, enlarged culturing 68～84h, to yeast saccharomyces cerevisiae content>=10 in fermentor tank
9individual/ml, the yeast saccharomyces cerevisiae list bacterium then fermentation obtained is prepared into the hypopus microbial dry powder through lyophilize.
The preparation of the hypopus microbial dry powder of described Bacillus licheniformis comprises the following steps:
Bacillus licheniformis strain on the slant medium that purifying is cultivated is seeded in the shaking flask that the activation medium that volume ratio is 15～30% is housed, the formula of described activation medium is peptone 8～12g/l, extractum carnis 1～5g/l and sodium-chlor 3～7g/l, in culture temperature, be 36～40 ℃, shaking flask rotating speed 180～220rpm cultivates 20～32h;
By activation culture, good Bacillus licheniformis strain is inoculated into and is equipped with in the fermentor tank that volume ratio is 50～70% fermention mediums, the volume inoculum size is 1～3%, the formula of described fermention medium is soybean cake powder 13～17g/l, Semen Maydis powder 13～17g/l, dipotassium hydrogen phosphate 0.05～0.15g/l, potassium primary phosphate 0.05～0.15g/l and calcium chloride 2～6g/l, in culture temperature, it is 36～40 ℃, fermentor tank mixing speed 200～220rpm cultivates 20～32h, Bacillus licheniformis content>=10 in fermentor tank
9individual/ml, the single bacterium drying of the Bacillus licheniformis then fermentation obtained is prepared into the hypopus microbial dry powder.
The preparation of the hypopus microbial dry powder of described nitrosomonus comprises the following steps:
Nitrosomonus bacterial classification on the slant medium that purifying is cultivated is seeded in the shaking flask that the activation medium that volume ratio is 15～30% is housed, and in culture temperature, is 24～28 ℃, and shaking flask rotating speed 120～140rpm cultivates 90～120h; By activation culture, good nitrosomonus bacterial classification is inoculated into and is equipped with in the fermentor tank that volume ratio is 50～70% fermention mediums, the volume inoculum size is 1～3%, in culture temperature, it is 24～28 ℃, fermentor tank mixing speed 120～140rpm cultivates 150～180h, nitrosomonus content>=10 in fermentor tank
8individual/ml, then the single bacterium of nitrosomonus fermentation obtained is prepared into the hypopus microbial dry powder through lyophilize, and the formula of substratum when described activation culture and fermentation is ammonium sulfate 1～3g/l, SODIUM PHOSPHATE, MONOBASIC 0.2～0.3g/l, manganous sulfate 0.005～0.015g/l, dipotassium hydrogen phosphate 0.5～1.0g/l, sal epsom 0.02～0.04g/l and calcium carbonate 3～7g/l.
The preparation of the hypopus microbial dry powder of described Vickers Nitrate bacteria comprises the following steps:
Vickers Nitrate bacteria bacterial classification on the slant medium that purifying is cultivated is seeded in the shaking flask that the activation medium that volume ratio is 15～30% is housed, and in culture temperature, is 24～28 ℃, and shaking flask rotating speed 120～140rpm cultivates 68～78h; By activation culture, good Vickers Nitrate bacteria bacterial classification is inoculated into and is equipped with in the fermentor tank that volume ratio is 50～70% fermention mediums, the volume inoculum size is 1～3%, in culture temperature, it is 24～28 ℃, fermentor tank mixing speed 120～140rpm cultivates 180～220h, Vickers Nitrate bacteria content>=10 in fermentor tank
8individual/ml, then the single bacterium of Vickers Nitrate bacteria fermentation obtained is prepared into the hypopus microbial dry powder through lyophilize, and the formula of substratum when described activation culture and fermentation is Sodium Nitrite 0.5～1.5g/l, sal epsom 0.01～0.05g/l, manganous sulfate 0.005～0.015g/l, dipotassium hydrogen phosphate 0.5～1.0g/l, sodium carbonate 0.5～1.5g/l, SODIUM PHOSPHATE, MONOBASIC 0.2～0.3g/l and calcium carbonate 0.5～1.5g/l.
Viride in the present invention can produce the multiple bioactive enzyme system that has, as: cellulase, chitinase, zytase etc.Viride is one of bacterial strain that institute's cellulase-producing activity is the highest; the cellulase produced has Degradation to crop; effect is very good; it is again a kind of resourceful antagonistic microbe; there is important effect in the plant pathology biological control; there is protection and treatment double effects, can effectively prevent and treat soil-borne disease.
Subtilis in the present invention is the important production bacterium of α-amylase and neutral protease, the subtilyne produced in subtilis thalli growth process, polymyxin, nystatin, linear gramicidins isoreactivity material, have obvious restraining effect to the conditioned pathogen of pathogenic bacterium or autogenous infection; Subtilis thalline self synthesizes the enzymes such as α-amylase, proteolytic enzyme, lipase, cellulase; Subtilis has very strong restraining effect to unwanted bacterias such as vibrios, intestinal bacteria and baculoviruss, secrete the function of a large amount of chitinases, chitinase can decompose the cell walls of pathogenic fungi and the Antifungi disease, decompose hazardous and noxious substances, purify water, residual bait, ight soil, organism etc. in decomposing pool, there is the short grained effect of rubbish in very strong cleaning water.
Bread mould in the present invention is very wide in distributed in nature, of many uses, and its amylase activity is very strong, is amylomyces commonly used in brewery industry, and has extremely strong steatolysis function.
Photosynthetic bacterium in the present invention is to using light to carry out photosynthetic microorganism as the energy, the organism that can under anaerobism illumination or aerobic dark condition, utilize occurring in nature, sulfide, ammonia etc. as the hydrogen donor carbon source of holding concurrently.In, seawater light at nature, common every milliliter contains more or less a hundred PSB bacterium, the thalline of photosynthetic bacterium is usingd the organism such as organic acid, amino acid, ammonia and carbohydrate and hydrogen sulfide as oxygen donator, obtain energy by photophosphorylation, can directly utilize degraded organic matter and hydrogen sulfide under illumination condition and make self to be bred in water, with advancing to have purified water body.
Yeast saccharomyces cerevisiae in the present invention can decompose the carbohydrate that utilizes in environment, hydrogen sulfide, ammonia etc., produce acidic substance in metabolic engineering, can effectively suppress pathogenic bacterium growths, and the yeast thalline can be used as the nutritive substance that other coordinate bacterium, be conducive to the fast breeding of composite flora.
Bacillus licheniformis in the present invention can produce the activity resistent material, and there is unique biology and take the oxygen mechanism of action by force, the growth and breeding that can suppress pathogenic bacterium, can decompose the hazardous and noxious substances in environment, there is stronger proteolytic enzyme, lipase, diastatic activity, can decompose starch, protein and lipid in environment.
Nitrosomonus in the present invention and Vickers Nitrate bacteria, this two classes bacterium is living together usually, has avoided the accumulation of nitrite in soil, is conducive to the body normal growth.Ammonia in soil or ammonium salt must just can change nitrate under the acting in conjunction of above two bacterioids, thereby increase the available nitrogen nutrition of plant.
Owing to having adopted technique scheme, the invention has the beneficial effects as follows:
The present invention is by viride, subtilis, bread mould, photosynthetic bacterium, yeast saccharomyces cerevisiae, Bacillus licheniformis, the bacterial classification of nitrosomonus and Vickers Nitrate bacteria carries out the hypopus microbial dry powder be prepared into after high-density culture, adopt rational proportion according to its purposes, the composite bacteria be mixed to get is for the processing of percolate, have the following advantages: (1) adopts rational proportion for the main component of food waste percolate, composite bacteria contains corresponding various single bacterium, the objectionable constituent that can degrade rapidly in percolate, to reducing ammonia nitrogen, COD, BOD etc. have unusual effect, (2) because organism is degraded rapidly, deodorizing effect is remarkable, uses the stink of percolate after 1 day significantly to alleviate, (3) reduce the food waste percolate water colour, (4) use food waste percolate after compound bacteria-fermented is processed can be discharged into sewage farm and process together with common sewage, reduced cost of sewage disposal, alleviated the load of sewage work, (5) the multiple thalline in the present invention's formula all has the fixedly effect of heavy metal, can effectively avoid heavy metal contamination soil resource and water resources in percolate.The present invention is for the processing of percolate, and easy to use, fermentative degradation is effective, and production cost is low.
Embodiment
Below in conjunction with specific embodiment, further set forth the present invention.
embodiment 1
Respectively the bacterial classification of viride, subtilis, bread mould, photosynthetic bacterium, yeast saccharomyces cerevisiae, Bacillus licheniformis, nitrosomonus and Vickers Nitrate bacteria is carried out to high-density culture:
(1) viride: the viride bacterial classification on the slant medium that purifying is cultivated is seeded in the shaking flask that the activation medium that volume ratio is 15% is housed, described activation medium is wort potato sucrose substratum, in culture temperature, be 30 ℃, shaking flask rotating speed 180rpm, activation culture 32h; By activation culture, good viride bacterial classification is inoculated into and is equipped with in the fermentor tank that volume ratio is 50% fermention medium, inoculum size is volume ratio 1%, the formula of described fermention medium is glucose 8g/l, corn cob 5g/l, calcium chloride 0.2g/l, sal epsom 0.1g/l, potassium primary phosphate 18g/l, yeast extract paste 8g/l, ferrous sulfate 3mg/l, zinc sulfate 1.0mg/l, cobalt chloride 3.5mg/l and manganous sulfate 1.2mg/l, in culture temperature, it is 30 ℃, fermentor tank mixing speed 140rpm, enlarged culturing 68h, to the mould content of fermentor tank Green wood>=10
8individual/ml, the viride list bacterium then fermentation obtained is prepared into the hypopus microbial dry powder through lyophilize.
(2) subtilis: the Bacillus subtilis strain on the slant medium that purifying is cultivated is seeded in the shaking flask that the activation medium that volume ratio is 15% is housed, the formula of described activation medium is glucose 18g/l, peptone 12g/l, sodium-chlor 3g/l and extractum carnis 0.2g/l, in culture temperature, be 37 ℃, shaking flask rotating speed 180rpm cultivates 20h; By activation culture, good Bacillus subtilis strain is inoculated into and is equipped with in the fermentor tank that volume ratio is 50% fermention medium, the volume inoculum size is 1%, the formula of described fermention medium is Semen Maydis powder 10g/l, glucose 3g/l, soybean cake powder 18g/l, fish meal 3g/l, calcium carbonate 5g/l, ammonium sulfate 0.8g/l, dipotassium hydrogen phosphate 0.1g/l, sal epsom 0.1g/l and manganous sulfate 0.1g/l, in culture temperature, it is 37 ℃, fermentor tank mixing speed 220rpm cultivates 20h, bacillus subtilis bacterial content>=10 in fermentor tank
9individual/ml, the single bacterium drying of the subtilis then fermentation obtained is prepared into the hypopus microbial dry powder.
(3) bread mould: the bread mould bacterial classification on the slant medium that purifying is cultivated is seeded in the shaking flask that the activation medium that volume ratio is 15% is housed, the potato juice 18g/l that the formula of described activation medium is 20wt%, glucose 1g/l, dipotassium hydrogen phosphate 1g/l, sal epsom 1g/l, VitB1 0.01g/l, in culture temperature, be 30 ℃, shaking flask rotating speed 180rpm cultivates 32h; By activation culture, good bread mould bacterial classification is inoculated into and is equipped with in the fermentor tank that volume ratio is 50% fermention medium, the volume inoculum size is 1%, the formula of described fermention medium is corn steep liquor 25g/l, sucrose 20g/l, urea 4g/l, dipotassium hydrogen phosphate 0.8g/l, sal epsom 0.3g/l, Repone K 0.3g/l and sweet oil 8g/l, in culture temperature, it is 30 ℃, fermentor tank mixing speed 140rpm cultivates 42h, bread mould content>=10 in fermentor tank
8individual/ml, the bread mould list bacterium then fermentation obtained is prepared into the hypopus microbial dry powder through lyophilize.
(4) photosynthetic bacterium: the photosynthetic bacterium bacterial classification on the slant medium that purifying is cultivated is seeded in the shaking flask that the substratum that volume ratio is 15% is housed, and in culture temperature, is 28 ℃, and shaking flask stirred once every 24 hours, cultivated 64h; By activation culture, good photosynthetic bacterium bacterial classification is inoculated into and is equipped with in the fermentor tank that volume ratio is 50% substratum, and the volume inoculum size is 6%, in culture temperature, is 28 ℃, every 24 hours, stir half an hour, mixing speed 140rpm, cultivate 100h, to photosynthetic bacterium content>=10 in fermentor tank
8individual/ml, then the single bacterium of photosynthetic bacterium fermentation obtained is prepared into the hypopus microbial dry powder through lyophilize, and the formula of substratum when described activation culture and fermentation is ammonium chloride 1g/l, dipotassium hydrogen phosphate 0.1g/l, sodium acetate 2g/l, sodium bicarbonate 1g/l, sodium-chlor 0.5g/l, yeast extract paste 0.1g/l and sal epsom 0.1g/l.
(5) yeast saccharomyces cerevisiae: the yeast saccharomyces cerevisiae bacterial classification on the slant medium that purifying is cultivated is seeded in the shaking flask that the substratum that volume ratio is 15% is housed, and in culture temperature, is 30 ℃, shaking flask rotating speed 140rpm, activation culture 32h; By activation culture, good yeast saccharomyces cerevisiae bacterial classification is inoculated into and is equipped with in the fermentor tank that volume ratio is 50% substratum, inoculum size is volume ratio 1%, substratum when described activation culture and fermentation is wort potato sucrose substratum, in culture temperature, it is 30 ℃, fermentor tank mixing speed 140rpm, enlarged culturing 68h, to yeast saccharomyces cerevisiae content>=10 in fermentor tank
9individual/ml, the yeast saccharomyces cerevisiae list bacterium then fermentation obtained is prepared into the hypopus microbial dry powder through lyophilize.
(6) Bacillus licheniformis: the Bacillus licheniformis strain on the slant medium that purifying is cultivated is seeded in the shaking flask that the activation medium that volume ratio is 15% is housed, the formula of described activation medium is peptone 8g/l, extractum carnis 1g/l and sodium-chlor 3g/l, in culture temperature, be 37 ℃, shaking flask rotating speed 180rpm cultivates 20h; By activation culture, good Bacillus licheniformis strain is inoculated into and is equipped with in the fermentor tank that volume ratio is 50% fermention medium, the volume inoculum size is 1%, the formula of described fermention medium is soybean cake powder 13g/l, Semen Maydis powder 13g/l, dipotassium hydrogen phosphate 0.05g/l, potassium primary phosphate 0.05g/l and calcium chloride 2g/l, in culture temperature, it is 37 ℃, fermentor tank mixing speed 200rpm cultivates 20h, Bacillus licheniformis content>=10 in fermentor tank
9individual/ml, the single bacterium drying of the Bacillus licheniformis then fermentation obtained is prepared into the hypopus microbial dry powder.
(7) nitrosomonus: the nitrosomonus bacterial classification on the slant medium that purifying is cultivated is seeded in the shaking flask that the activation medium that volume ratio is 15% is housed, and in culture temperature, is 28 ℃, and shaking flask rotating speed 120rpm cultivates 90h; By activation culture, good nitrosomonus bacterial classification is inoculated into and is equipped with in the fermentor tank that volume ratio is 50% fermention medium, the volume inoculum size is 1%, in culture temperature, be 28 ℃, fermentor tank mixing speed 120rpm cultivates 150h, nitrosomonus content>=10 in fermentor tank
8individual/ml, then the single bacterium of nitrosomonus fermentation obtained is prepared into the hypopus microbial dry powder through lyophilize, and the formula of substratum when described activation culture and fermentation is ammonium sulfate 1g/l, SODIUM PHOSPHATE, MONOBASIC 0.2g/l, manganous sulfate 0.005g/l, dipotassium hydrogen phosphate 0.5g/l, sal epsom 0.02g/l and calcium carbonate 3g/l.
(8) Vickers Nitrate bacteria: the Vickers Nitrate bacteria bacterial classification on the slant medium that purifying is cultivated is seeded in the shaking flask that the activation medium that volume ratio is 15% is housed, and in culture temperature, is 28 ℃, and shaking flask rotating speed 120rpm cultivates 78h; By activation culture, good Vickers Nitrate bacteria bacterial classification is inoculated into and is equipped with in the fermentor tank that volume ratio is 50% fermention medium, the volume inoculum size is 1%, in culture temperature, be 28 ℃, fermentor tank mixing speed 120rpm cultivates 180h, Vickers Nitrate bacteria content>=10 in fermentor tank
8individual/ml, then the single bacterium of Vickers Nitrate bacteria fermentation obtained is prepared into the hypopus microbial dry powder through lyophilize, and the formula of substratum when described activation culture and fermentation is Sodium Nitrite 0.5g/l, sal epsom 0.01g/l, manganous sulfate 0.005g/l, dipotassium hydrogen phosphate 0.5g/l, sodium carbonate 0.5g/l, SODIUM PHOSPHATE, MONOBASIC 0.2g/l and calcium carbonate 0.5g/l.
The various hypopus microbial dry powders that high-density culture is obtained, weight ratio according to 10 parts of 5 parts of virides, 10 parts of subtilises, 20 parts of bread moulds, 10 parts of photosynthetic bacteriums, 7 parts of yeast saccharomyces cerevisiaes, 10 parts of Bacillus licheniformis, 10 parts of nitrosomonus and Vickers Nitrate bacterias, be mixed to get the composite bacteria for the treatment of food waste percolate.
embodiment 2
Respectively the bacterial classification of viride, subtilis, bread mould, photosynthetic bacterium, yeast saccharomyces cerevisiae, Bacillus licheniformis, nitrosomonus and Vickers Nitrate bacteria is carried out to high-density culture:
(1) viride: the viride bacterial classification on the slant medium that purifying is cultivated is seeded in the shaking flask that the activation medium that volume ratio is 30% is housed, described activation medium is wort potato sucrose substratum, in culture temperature, be 30 ℃, shaking flask rotating speed 140rpm, activation culture 48h; By activation culture, good viride bacterial classification is inoculated into and is equipped with in the fermentor tank that volume ratio is 70% fermention medium, inoculum size is volume ratio 3%, the formula of described fermention medium is glucose 12g/l, corn cob 6g/l, calcium chloride 0.6g/l, sal epsom 0.5g/l, potassium primary phosphate 22g/l, yeast extract paste 12g/l, ferrous sulfate 7mg/l, zinc sulfate 1.8mg/l, cobalt chloride 4.0mg/l and manganous sulfate 1.8mg/l, in culture temperature, it is 30 ℃, fermentor tank mixing speed 180rpm, enlarged culturing 68h, to the mould content of fermentor tank Green wood>=10
8individual/ml, the viride list bacterium then fermentation obtained is prepared into the hypopus microbial dry powder through lyophilize.
(2) subtilis: the Bacillus subtilis strain on the slant medium that purifying is cultivated is seeded in the shaking flask that the activation medium that volume ratio is 30% is housed, the formula of described activation medium is glucose 22g/l, peptone 18g/l, sodium-chlor 7g/l and extractum carnis 0.8g/l, in culture temperature, be 37 ℃, shaking flask rotating speed 220rpm cultivates 20h; By activation culture, good Bacillus subtilis strain is inoculated into and is equipped with in the fermentor tank that volume ratio is 70% fermention medium, the volume inoculum size is 3%, the formula of described fermention medium is Semen Maydis powder 15g/l, glucose 8g/l, soybean cake powder 22g/l, fish meal 8g/l, calcium carbonate 9g/l, ammonium sulfate 1.2g/l, dipotassium hydrogen phosphate 0.5g/l, sal epsom 0.3g/l and manganous sulfate 0.3g/l, in culture temperature, it is 37 ℃, fermentor tank mixing speed 220rpm cultivates 32h, bacillus subtilis bacterial content>=10 in fermentor tank
9individual/ml, the single bacterium drying of the subtilis then fermentation obtained is prepared into the hypopus microbial dry powder.
(3) bread mould: the bread mould bacterial classification on the slant medium that purifying is cultivated is seeded in the shaking flask that the activation medium that volume ratio is 30% is housed, the potato juice 22g/l that the formula of described activation medium is 20wt%, glucose 5g/l, dipotassium hydrogen phosphate 5g/l, sal epsom 2g/l, VitB1 0.05g/l, in culture temperature, be 30 ℃, shaking flask rotating speed 180rpm cultivates 42h; By activation culture, good bread mould bacterial classification is inoculated into and is equipped with in the fermentor tank that volume ratio is 70% fermention medium, the volume inoculum size is 3%, the formula of described fermention medium is corn steep liquor 35g/l, sucrose 20g/l, urea 8g/l, dipotassium hydrogen phosphate 1.2g/l, sal epsom 0.8g/l, Repone K 0.8g/l and sweet oil 12g/l, in culture temperature, it is 30 ℃, fermentor tank mixing speed 180rpm cultivates 54h, bread mould content>=10 in fermentor tank
8individual/ml, the bread mould list bacterium then fermentation obtained is prepared into the hypopus microbial dry powder through lyophilize.
(4) photosynthetic bacterium: the photosynthetic bacterium bacterial classification on the slant medium that purifying is cultivated is seeded in the shaking flask that the substratum that volume ratio is 30% is housed, and in culture temperature, is 30 ℃, and shaking flask stirred once every 24 hours, cultivated 84h; By activation culture, good photosynthetic bacterium bacterial classification is inoculated into and is equipped with in the fermentor tank that volume ratio is 70% substratum, and the volume inoculum size is 10%, in culture temperature, is 30 ℃, every 24 hours, stir half an hour, mixing speed 180rpm, cultivate 150h, to photosynthetic bacterium content>=10 in fermentor tank
8individual/ml, then the single bacterium of photosynthetic bacterium fermentation obtained is prepared into the hypopus microbial dry powder through lyophilize, and the formula of substratum when described activation culture and fermentation is ammonium chloride 3g/l, dipotassium hydrogen phosphate 0.3g/l, sodium acetate 6g/l, sodium bicarbonate 3g/l, sodium-chlor 1.5g/l, yeast extract paste 0.2g/l and sal epsom 0.3g/l.
(5) yeast saccharomyces cerevisiae: the yeast saccharomyces cerevisiae bacterial classification on the slant medium that purifying is cultivated is seeded in the shaking flask that the substratum that volume ratio is 30% is housed, and in culture temperature, is 30 ℃, shaking flask rotating speed 180rpm, activation culture 48h; By activation culture, good yeast saccharomyces cerevisiae bacterial classification is inoculated into and is equipped with in the fermentor tank that volume ratio is 70% substratum, inoculum size is volume ratio 3%, substratum when described activation culture and fermentation is wort potato sucrose substratum, in culture temperature, it is 30 ℃, fermentor tank mixing speed 180rpm, enlarged culturing 84h, to yeast saccharomyces cerevisiae content>=10 in fermentor tank
9individual/ml, the yeast saccharomyces cerevisiae list bacterium then fermentation obtained is prepared into the hypopus microbial dry powder through lyophilize.
(6) Bacillus licheniformis: the Bacillus licheniformis strain on the slant medium that purifying is cultivated is seeded in the shaking flask that the activation medium that volume ratio is 30% is housed, the formula of described activation medium is peptone 12g/l, extractum carnis 5g/l and sodium-chlor 7g/l, in culture temperature, be 37 ℃, shaking flask rotating speed 220rpm cultivates 32h; By activation culture, good Bacillus licheniformis strain is inoculated into and is equipped with in the fermentor tank that volume ratio is 70% fermention medium, the volume inoculum size is 3%, the formula of described fermention medium is soybean cake powder 17g/l, Semen Maydis powder 17g/l, dipotassium hydrogen phosphate 0.15g/l, potassium primary phosphate 0.15g/l and calcium chloride 6g/l, in culture temperature, it is 37 ℃, fermentor tank mixing speed 220rpm cultivates 32h, Bacillus licheniformis content>=10 in fermentor tank
9individual/ml, the single bacterium drying of the Bacillus licheniformis then fermentation obtained is prepared into the hypopus microbial dry powder.
(7) nitrosomonus: the nitrosomonus bacterial classification on the slant medium that purifying is cultivated is seeded in the shaking flask that the activation medium that volume ratio is 30% is housed, and in culture temperature, is 28 ℃, and shaking flask rotating speed 140rpm cultivates 120h; By activation culture, good nitrosomonus bacterial classification is inoculated into and is equipped with in the fermentor tank that volume ratio is 70% fermention medium, the volume inoculum size is 3%, in culture temperature, be 28 ℃, fermentor tank mixing speed 140rpm cultivates 180h, nitrosomonus content>=10 in fermentor tank
8individual/ml, then the single bacterium of nitrosomonus fermentation obtained is prepared into the hypopus microbial dry powder through lyophilize, and the formula of substratum when described activation culture and fermentation is ammonium sulfate 3g/l, SODIUM PHOSPHATE, MONOBASIC 0.3g/l, manganous sulfate 0.015g/l, dipotassium hydrogen phosphate 1.0g/l, sal epsom 0.04g/l and calcium carbonate 7g/l.
(8) Vickers Nitrate bacteria: the Vickers Nitrate bacteria bacterial classification on the slant medium that purifying is cultivated is seeded in the shaking flask that the activation medium that volume ratio is 30% is housed, and in culture temperature, is 28 ℃, and shaking flask rotating speed 140rpm cultivates 78h; By activation culture, good Vickers Nitrate bacteria bacterial classification is inoculated into and is equipped with in the fermentor tank that volume ratio is 70% fermention medium, the volume inoculum size is 3%, in culture temperature, be 28 ℃, fermentor tank mixing speed 140rpm cultivates 220h, Vickers Nitrate bacteria content>=10 in fermentor tank
8individual/ml, then the single bacterium of Vickers Nitrate bacteria fermentation obtained is prepared into the hypopus microbial dry powder through lyophilize, and the formula of substratum when described activation culture and fermentation is Sodium Nitrite 1.5g/l, sal epsom 0.05g/l, manganous sulfate 0.015g/l, dipotassium hydrogen phosphate 1.0g/l, sodium carbonate 1.5g/l, SODIUM PHOSPHATE, MONOBASIC 0.3g/l and calcium carbonate 1.5g/l.
The various hypopus microbial dry powders that high-density culture is obtained, weight ratio according to 20 parts of 15 parts of virides, 15 parts of subtilises, 30 parts of bread moulds, 15 parts of photosynthetic bacteriums, 15 parts of yeast saccharomyces cerevisiaes, 15 parts of Bacillus licheniformis, 20 parts of nitrosomonus and Vickers Nitrate bacterias, be mixed to get the composite bacteria for the treatment of food waste percolate.
embodiment 3
Respectively the bacterial classification of viride, subtilis, bread mould, photosynthetic bacterium, yeast saccharomyces cerevisiae, Bacillus licheniformis, nitrosomonus and Vickers Nitrate bacteria is carried out to high-density culture:
(1) viride: the viride bacterial classification on the slant medium that purifying is cultivated is seeded in the shaking flask that the activation medium that volume ratio is 20% is housed, described activation medium is wort potato sucrose substratum, in culture temperature, be 30 ℃, shaking flask rotating speed 160rpm, activation culture 36h; By activation culture, good viride bacterial classification is inoculated into and is equipped with in the fermentor tank that volume ratio is 60% fermention medium, inoculum size is volume ratio 2%, the formula of described fermention medium is glucose 10g/l, corn cob 5.5g/l, calcium chloride 0.4g/l, sal epsom 0.3g/l, potassium primary phosphate 20g/l, yeast extract paste 10g/l, ferrous sulfate 5mg/l, zinc sulfate 1.5mg/l, cobalt chloride 3.7mg/l and manganous sulfate 1.6mg/l, in culture temperature, it is 30 ℃, fermentor tank mixing speed 160rpm, enlarged culturing 72h, to the mould content of fermentor tank Green wood>=10
8individual/ml, the viride list bacterium then fermentation obtained is prepared into the hypopus microbial dry powder through lyophilize.
(2) subtilis: the Bacillus subtilis strain on the slant medium that purifying is cultivated is seeded in the shaking flask that the activation medium that volume ratio is 20% is housed, the formula of described activation medium is glucose 20g/l, peptone 16g/l, sodium-chlor 5g/l and extractum carnis 0.6g/l, in culture temperature, be 37 ℃, shaking flask rotating speed 200rpm cultivates 24h; By activation culture, good Bacillus subtilis strain is inoculated into and is equipped with in the fermentor tank that volume ratio is 60% fermention medium, the volume inoculum size is 2%, the formula of described fermention medium is Semen Maydis powder 12g/l, glucose 5g/l, soybean cake powder 20g/l, fish meal 5g/l, calcium carbonate 7g/l, ammonium sulfate 1.0g/l, dipotassium hydrogen phosphate 0.3g/l, sal epsom 0.2g/l and manganous sulfate 0.2g/l, in culture temperature, it is 37 ℃, fermentor tank mixing speed 210rpm cultivates 24h, bacillus subtilis bacterial content>=10 in fermentor tank
9individual/ml, the single bacterium drying of the subtilis then fermentation obtained is prepared into the hypopus microbial dry powder.
(3) bread mould: the bread mould bacterial classification on the slant medium that purifying is cultivated is seeded in the shaking flask that the activation medium that volume ratio is 20% is housed, the potato juice 20g/l that the formula of described activation medium is 20wt%, glucose 3g/l, dipotassium hydrogen phosphate 3g/l, sal epsom 1.5g/l, VitB1 0.02g/l, in culture temperature, be 30 ℃, shaking flask rotating speed 160rpm cultivates 36h; By activation culture, good bread mould bacterial classification is inoculated into and is equipped with in the fermentor tank that volume ratio is 60% fermention medium, the volume inoculum size is 2%, the formula of described fermention medium is corn steep liquor 30g/l, sucrose 20g/l, urea 6g/l, dipotassium hydrogen phosphate 1.0g/l, sal epsom 0.5g/l, Repone K 0.5g/l and sweet oil 10g/l, in culture temperature, it is 30 ℃, fermentor tank mixing speed 160rpm cultivates 48h, bread mould content>=10 in fermentor tank
8individual/ml, the bread mould list bacterium then fermentation obtained is prepared into the hypopus microbial dry powder through lyophilize.
(4) photosynthetic bacterium: the photosynthetic bacterium bacterial classification on the slant medium that purifying is cultivated is seeded in the shaking flask that the substratum that volume ratio is 20% is housed, and in culture temperature, is 30 ℃, and shaking flask stirred once every 24 hours, cultivated 72h; By activation culture, good photosynthetic bacterium bacterial classification is inoculated into and is equipped with in the fermentor tank that volume ratio is 60% substratum, and the volume inoculum size is 8%, in culture temperature, is 30 ℃, every 24 hours, stir half an hour, mixing speed 160rpm, cultivate 120h, to photosynthetic bacterium content>=10 in fermentor tank
8individual/ml, then the single bacterium of photosynthetic bacterium fermentation obtained is prepared into the hypopus microbial dry powder through lyophilize, and the formula of substratum when described activation culture and fermentation is ammonium chloride 2g/l, dipotassium hydrogen phosphate 0.2g/l, sodium acetate 4g/l, sodium bicarbonate 2g/l, sodium-chlor 1.0g/l, yeast extract paste 0.15g/l and sal epsom 0.2g/l.
(5) yeast saccharomyces cerevisiae: the yeast saccharomyces cerevisiae bacterial classification on the slant medium that purifying is cultivated is seeded in the shaking flask that the substratum that volume ratio is 20% is housed, and in culture temperature, is 30 ℃, shaking flask rotating speed 160rpm, activation culture 38h; By activation culture, good yeast saccharomyces cerevisiae bacterial classification is inoculated into and is equipped with in the fermentor tank that volume ratio is 60% substratum, inoculum size is volume ratio 2%, substratum when described activation culture and fermentation is wort potato sucrose substratum, in culture temperature, it is 30 ℃, fermentor tank mixing speed 160rpm, enlarged culturing 72h, to yeast saccharomyces cerevisiae content>=10 in fermentor tank
9individual/ml, the yeast saccharomyces cerevisiae list bacterium then fermentation obtained is prepared into the hypopus microbial dry powder through lyophilize.
(6) Bacillus licheniformis: the Bacillus licheniformis strain on the slant medium that purifying is cultivated is seeded in the shaking flask that the activation medium that volume ratio is 20% is housed, the formula of described activation medium is peptone 10g/l, extractum carnis 3g/l and sodium-chlor 5g/l, in culture temperature, be 37 ℃, shaking flask rotating speed 200rpm cultivates 24h; By activation culture, good Bacillus licheniformis strain is inoculated into and is equipped with in the fermentor tank that volume ratio is 60% fermention medium, the volume inoculum size is 2%, the formula of described fermention medium is soybean cake powder 15g/l, Semen Maydis powder 15g/l, dipotassium hydrogen phosphate 0.1g/l, potassium primary phosphate 0.1g/l and calcium chloride 4g/l, in culture temperature, it is 37 ℃, fermentor tank mixing speed 210rpm cultivates 24h, Bacillus licheniformis content>=10 in fermentor tank
9individual/ml, the single bacterium drying of the Bacillus licheniformis then fermentation obtained is prepared into the hypopus microbial dry powder.
(7) nitrosomonus: the nitrosomonus bacterial classification on the slant medium that purifying is cultivated is seeded in the shaking flask that the activation medium that volume ratio is 20% is housed, and in culture temperature, is 28 ℃, and shaking flask rotating speed 130rpm cultivates 100h; By activation culture, good nitrosomonus bacterial classification is inoculated into and is equipped with in the fermentor tank that volume ratio is 60% fermention medium, the volume inoculum size is 2%, in culture temperature, be 28 ℃, fermentor tank mixing speed 130rpm cultivates 160h, nitrosomonus content>=10 in fermentor tank
8individual/ml, then the single bacterium of nitrosomonus fermentation obtained is prepared into the hypopus microbial dry powder through lyophilize, and the formula of substratum when described activation culture and fermentation is ammonium sulfate 2g/l, SODIUM PHOSPHATE, MONOBASIC 0.25g/l, manganous sulfate 0.01g/l, dipotassium hydrogen phosphate 0.8g/l, sal epsom 0.03g/l and calcium carbonate 5g/l.
(8) Vickers Nitrate bacteria: the Vickers Nitrate bacteria bacterial classification on the slant medium that purifying is cultivated is seeded in the shaking flask that the activation medium that volume ratio is 20% is housed, and in culture temperature, is 28 ℃, and shaking flask rotating speed 130rpm cultivates 72h; By activation culture, good Vickers Nitrate bacteria bacterial classification is inoculated into and is equipped with in the fermentor tank that volume ratio is 60% fermention medium, the volume inoculum size is 2%, in culture temperature, be 28 ℃, fermentor tank mixing speed 130rpm cultivates 200h, Vickers Nitrate bacteria content>=10 in fermentor tank
8individual/ml, then the single bacterium of Vickers Nitrate bacteria fermentation obtained is prepared into the hypopus microbial dry powder through lyophilize, and the formula of substratum when described activation culture and fermentation is Sodium Nitrite 1.0g/l, sal epsom 0.03g/l, manganous sulfate 0.01g/l, dipotassium hydrogen phosphate 0.8g/l, sodium carbonate 1.0g/l, SODIUM PHOSPHATE, MONOBASIC 0.25g/l and calcium carbonate 1.0g/l.
The various hypopus microbial dry powders that high-density culture is obtained, weight ratio according to 15 parts of 10 parts of virides, 12 parts of subtilises, 25 parts of bread moulds, 12 parts of photosynthetic bacteriums, 11 parts of yeast saccharomyces cerevisiaes, 12 parts of Bacillus licheniformis, 15 parts of nitrosomonus and Vickers Nitrate bacterias, be mixed to get the composite bacteria for the treatment of food waste percolate.
Claims (10)
1. the composite bacteria for the treatment of food waste percolate is characterized in that mainly by the mixed raw material of following weight part is standby, forming:
5～15 parts of virides,
10～15 parts of subtilises,
20～30 parts of bread moulds,
10～15 parts of photosynthetic bacteriums,
7～15 parts of yeast saccharomyces cerevisiaes,
10～15 parts of Bacillus licheniformis,
10～20 parts of nitrosomonus,
10～20 parts of Vickers Nitrate bacterias.
2. the preparation method of the composite bacteria for the treatment of food waste percolate as claimed in claim 1 is characterized in that comprising the following steps:
Respectively by viride, subtilis, bread mould, photosynthetic bacterium, yeast saccharomyces cerevisiae, Bacillus licheniformis, the bacterial classification of nitrosomonus and Vickers Nitrate bacteria carries out high-density culture, at first be seeded to activation culture in the shaking flask that the substratum that volume ratio is 15～30% is housed, cultured bacterial classification is inoculated in fermentor tank to the enlarged culturing of being fermented, various single bacterium that enlarged culturing is obtained dehydrates, be prepared into the hypopus microbial dry powder, then according to 5～15 parts of virides, 10～15 parts of subtilises, 20～30 parts of bread moulds, 10～15 parts of photosynthetic bacteriums, 7～15 parts of yeast saccharomyces cerevisiaes, 10～15 parts of Bacillus licheniformis, the weight ratio that 10～20 parts of nitrosomonus and Vickers Nitrate bacteria are 10～20 parts, be mixed to get the composite bacteria for the treatment of food waste percolate.
3. the preparation method of the composite bacteria for the treatment of food waste percolate as claimed in claim 2 is characterized in that the preparation of described viride hypopus microbial dry powder comprises the following steps:
Viride bacterial classification on the slant medium that purifying is cultivated is seeded in the shaking flask that the activation medium that volume ratio is 15～30% is housed, described activation medium is wort potato sucrose substratum, in culture temperature, be 28～30 ℃, shaking flask rotating speed 140～180rpm, activation culture 32～48h;
By activation culture, good viride bacterial classification is inoculated into and is equipped with in the fermentor tank that volume ratio is 50～70% fermention mediums, inoculum size is volume ratio 1～3%, the formula of described fermention medium is glucose 8～12g/l, corn cob 5～6g/l, calcium chloride 0.2～0.6g/l, sal epsom 0.1～0.5g/l, potassium primary phosphate 18～22g/l, yeast extract paste 8～12g/l, ferrous sulfate 3～7mg/l, zinc sulfate 1.0～1.8mg/l, cobalt chloride 3.5～4.0mg/l and manganous sulfate 1.2～1.8mg/l, in culture temperature, it is 28～30 ℃, fermentor tank mixing speed 140～180rpm, enlarged culturing 68～84h, to the mould content of fermentor tank Green wood>=10
8individual/ml, the viride list bacterium then fermentation obtained is prepared into the hypopus microbial dry powder through lyophilize.
4. the preparation method of the composite bacteria for the treatment of food waste percolate as claimed in claim 2 is characterized in that the preparation of the hypopus microbial dry powder of described subtilis comprises the following steps:
Bacillus subtilis strain on the slant medium that purifying is cultivated is seeded in the shaking flask that the activation medium that volume ratio is 15～30% is housed, the formula of described activation medium is glucose 18～22g/l, peptone 12～18g/l, sodium-chlor 3～7g/l and extractum carnis 0.2～0.8g/l, in culture temperature, be 36～40 ℃, shaking flask rotating speed 180～220rpm cultivates 20～32h;
By activation culture, good Bacillus subtilis strain is inoculated into and is equipped with in the fermentor tank that volume ratio is 50～70% fermention mediums, the volume inoculum size is 1～3%, the formula of described fermention medium is Semen Maydis powder 10～15g/l, glucose 3～8g/l, soybean cake powder 18～22g/l, fish meal 3～8g/l, calcium carbonate 5～9g/l, ammonium sulfate 0.8～1.2g/l, dipotassium hydrogen phosphate 0.1～0.5g/l, sal epsom 0.1～0.3g/l and manganous sulfate 0.1～0.3g/l, in culture temperature, it is 36～40 ℃, fermentor tank mixing speed 200～220rpm cultivates 20～32h, bacillus subtilis bacterial content>=10 in fermentor tank
9individual/ml, the single bacterium drying of the subtilis then fermentation obtained is prepared into the hypopus microbial dry powder.
5. the preparation method of the composite bacteria for the treatment of food waste percolate as claimed in claim 2, it is characterized in that: the preparation of the hypopus microbial dry powder of described bread mould comprises the following steps:
Bread mould bacterial classification on the slant medium that purifying is cultivated is seeded in the shaking flask that the activation medium that volume ratio is 15～30% is housed, potato juice 18～22g/l that the formula of described activation medium is 20wt%, glucose 1～5g/l, dipotassium hydrogen phosphate 1～5g/l, sal epsom 1～2g/l, VitB1 0.01～0.05g/l, in culture temperature, be 28～30 ℃, shaking flask rotating speed 140～180rpm cultivates 32～42h;
By activation culture, good bread mould bacterial classification is inoculated into and is equipped with in the fermentor tank that volume ratio is 50～70% fermention mediums, the volume inoculum size is 1～3%, the formula of described fermention medium is corn steep liquor 25～35g/l, sucrose 20～40g/l, urea 4～8g/l, dipotassium hydrogen phosphate 0.8～1.2g/l, sal epsom 0.3～0.8g/l, Repone K 0.3～0.8g/l and sweet oil 8～12g/l, in culture temperature, it is 28～30 ℃, fermentor tank mixing speed 140～180rpm cultivates 42～54h, bread mould content>=10 in fermentor tank
8individual/ml, the bread mould list bacterium then fermentation obtained is prepared into the hypopus microbial dry powder through lyophilize.
6. the preparation method of the composite bacteria for the treatment of food waste percolate as claimed in claim 2, it is characterized in that: the preparation of the hypopus microbial dry powder of described photosynthetic bacterium comprises the following steps:
Photosynthetic bacterium bacterial classification on the slant medium that purifying is cultivated is seeded in the shaking flask that the substratum that volume ratio is 15～30% is housed, and in culture temperature, is 28～30 ℃, and shaking flask stirred once every 24 hours, cultivated 64～84h; By activation culture, good photosynthetic bacterium bacterial classification is inoculated into and is equipped with in the fermentor tank that volume ratio is 50～70% substratum, the volume inoculum size is 6～10%, in culture temperature, it is 28～30 ℃, every 24 hours, stir half an hour, mixing speed 140～180rpm, cultivate 100～150h, to photosynthetic bacterium content>=10 in fermentor tank
8individual/ml, then the single bacterium of photosynthetic bacterium fermentation obtained is prepared into the hypopus microbial dry powder through lyophilize, and the formula of substratum when described activation culture and fermentation is ammonium chloride 1～3g/l, dipotassium hydrogen phosphate 0.1～0.3g/l, sodium acetate 2～6g/l, sodium bicarbonate 1～3g/l, sodium-chlor 0.5～1.5g/l, yeast extract paste 0.1～0.2g/l and sal epsom 0.1～0.3g/l.
7. the preparation method of the composite bacteria for the treatment of food waste percolate as claimed in claim 2 is characterized in that the preparation of described yeast saccharomyces cerevisiae hypopus microbial dry powder comprises the following steps:
Yeast saccharomyces cerevisiae bacterial classification on the slant medium that purifying is cultivated is seeded in the shaking flask that the substratum that volume ratio is 15～30% is housed, and in culture temperature, is 28～30 ℃, shaking flask rotating speed 140～180rpm, activation culture 32～48h; By activation culture, good yeast saccharomyces cerevisiae bacterial classification is inoculated into and is equipped with in the fermentor tank that volume ratio is 50～70% substratum, inoculum size is volume ratio 1～3%, substratum when described activation culture and fermentation is wort potato sucrose substratum, in culture temperature, it is 28～30 ℃, fermentor tank mixing speed 140～180rpm, enlarged culturing 68～84h, to yeast saccharomyces cerevisiae content>=10 in fermentor tank
9individual/ml, the yeast saccharomyces cerevisiae list bacterium then fermentation obtained is prepared into the hypopus microbial dry powder through lyophilize.
8. the preparation method of the composite bacteria for the treatment of food waste percolate as claimed in claim 2 is characterized in that the preparation of the hypopus microbial dry powder of described Bacillus licheniformis comprises the following steps:
Bacillus licheniformis strain on the slant medium that purifying is cultivated is seeded in the shaking flask that the activation medium that volume ratio is 15～30% is housed, the formula of described activation medium is peptone 8～12g/l, extractum carnis 1～5g/l and sodium-chlor 3～7g/l, in culture temperature, be 36～40 ℃, shaking flask rotating speed 180～220rpm cultivates 20～32h;
By activation culture, good Bacillus licheniformis strain is inoculated into and is equipped with in the fermentor tank that volume ratio is 50～70% fermention mediums, the volume inoculum size is 1～3%, the formula of described fermention medium is soybean cake powder 13～17g/l, Semen Maydis powder 13～17g/l, dipotassium hydrogen phosphate 0.05～0.15g/l, potassium primary phosphate 0.05～0.15g/l and calcium chloride 2～6g/l, in culture temperature, it is 36～40 ℃, fermentor tank mixing speed 200～220rpm cultivates 20～32h, Bacillus licheniformis content>=10 in fermentor tank
9individual/ml, the single bacterium drying of the Bacillus licheniformis then fermentation obtained is prepared into the hypopus microbial dry powder.
9. the preparation method of the composite bacteria for the treatment of food waste percolate as claimed in claim 2, it is characterized in that: the preparation of the hypopus microbial dry powder of described nitrosomonus comprises the following steps:
Nitrosomonus bacterial classification on the slant medium that purifying is cultivated is seeded in the shaking flask that the activation medium that volume ratio is 15～30% is housed, and in culture temperature, is 24～28 ℃, and shaking flask rotating speed 120～140rpm cultivates 90～120h; By activation culture, good nitrosomonus bacterial classification is inoculated into and is equipped with in the fermentor tank that volume ratio is 50～70% fermention mediums, the volume inoculum size is 1～3%, in culture temperature, it is 24～28 ℃, fermentor tank mixing speed 120～140rpm cultivates 150～180h, nitrosomonus content>=10 in fermentor tank
8individual/ml, then the single bacterium of nitrosomonus fermentation obtained is prepared into the hypopus microbial dry powder through lyophilize, and the formula of substratum when described activation culture and fermentation is ammonium sulfate 1～3g/l, SODIUM PHOSPHATE, MONOBASIC 0.2～0.3g/l, manganous sulfate 0.005～0.015g/l, dipotassium hydrogen phosphate 0.5～1.0g/l, sal epsom 0.02～0.04g/l and calcium carbonate 3～7g/l.
10. the preparation method of the composite bacteria for the treatment of food waste percolate as claimed in claim 2, it is characterized in that: the preparation of the hypopus microbial dry powder of described Vickers Nitrate bacteria comprises the following steps:
Vickers Nitrate bacteria bacterial classification on the slant medium that purifying is cultivated is seeded in the shaking flask that the activation medium that volume ratio is 15～30% is housed, and in culture temperature, is 24～28 ℃, and shaking flask rotating speed 120～140rpm cultivates 68～78h; By activation culture, good Vickers Nitrate bacteria bacterial classification is inoculated into and is equipped with in the fermentor tank that volume ratio is 50～70% fermention mediums, the volume inoculum size is 1～3%, in culture temperature, it is 24～28 ℃, fermentor tank mixing speed 120～140rpm cultivates 180～220h, Vickers Nitrate bacteria content>=10 in fermentor tank
8individual/ml, then the single bacterium of Vickers Nitrate bacteria fermentation obtained is prepared into the hypopus microbial dry powder through lyophilize, and the formula of substratum when described activation culture and fermentation is Sodium Nitrite 0.5～1.5g/l, sal epsom 0.01～0.05g/l, manganous sulfate 0.005～0.015g/l, dipotassium hydrogen phosphate 0.5～1.0g/l, sodium carbonate 0.5～1.5g/l, SODIUM PHOSPHATE, MONOBASIC 0.2～0.3g/l and calcium carbonate 0.5～1.5g/l.
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| CN102976801B (en) * | 2012-12-13 | 2014-06-11 | 广州舒国生物科技有限公司 | Method for producing functional microorganism organic fertilizer by using food residue |
| CN103468614B (en) * | 2013-09-16 | 2015-05-20 | 中国人民解放军总后勤部军需装备研究所 | Kitchen waste decomposition bacterial agent and preparation method thereof |
| CN104248911B (en) * | 2014-05-13 | 2016-08-31 | 江苏绿华生物工程有限公司 | A kind of biological deodorant and preparation method thereof |
| CN104458734B (en) * | 2014-12-19 | 2017-01-25 | 中国环境科学研究院 | Method for determining reduction potential of compost and mineralized refuse |
| CN104862244B (en) * | 2015-03-26 | 2017-12-19 | 北京北华中清环境工程技术有限公司 | A kind of high efficiency composition microbial inoculum for removing the oil wastewater COD containing mixing and its application |
| CN105502665A (en) * | 2015-11-25 | 2016-04-20 | 广西金威生物技术有限公司 | Garbage leachate wastewater biological comprehensive treatment method |
| CN109809840B (en) * | 2016-08-28 | 2021-08-20 | 刘明 | Municipal refuse treatment method |
| CN108102973A (en) * | 2018-01-31 | 2018-06-01 | 福建双环能源科技股份有限公司 | A kind of destructor plant percolate deodorization special bacterium and its application method |
| CN108772408A (en) * | 2018-06-25 | 2018-11-09 | 成都市市政工程（集团）有限责任公司 | Municipal refuse comprehensive processing technique |
| CN109879564B (en) * | 2019-04-02 | 2023-04-25 | 四川健全环境集团有限公司 | Biological treatment method of municipal sludge |
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