CN114181856B

CN114181856B - Microbial combination capable of effectively inhibiting odor production of biomass fermentation from source and application thereof

Info

Publication number: CN114181856B
Application number: CN202111496500.5A
Authority: CN
Inventors: 刘玉涛; 马海霞; 万和川; 李骅; 赵三琴; 顾家冰
Original assignee: Nanjing Agricultural University
Current assignee: Nanjing Agricultural University
Priority date: 2021-11-30
Filing date: 2021-11-30
Publication date: 2023-12-15
Anticipated expiration: 2041-11-30
Also published as: CN114181856A

Abstract

The invention provides a microbial combination for effectively inhibiting odor production of biomass fermentation from a source and application thereof, and relates to the technical field of gas purification. The active ingredients of the odor inhibiting microbial inoculum respectively comprise saccharomycetes from kitchen waste and decomposed bacteria and nitrifying bacteria from livestock and poultry manure; can ensure the biological safety of the plant, responds to the concept of environmental protection and emission reduction, and has wide application range. The invention provides a use method based on the combined microbial inoculum, which increases the infiltration of the microbial inoculum on biomass and improves the probability of microorganisms entering the biomass, thereby achieving the purposes of promoting the propagation of the microorganisms and inhibiting the generation of odor. The functions of the microorganisms in the complex flora have complementarity, thereby ensuring high efficiency and thoroughness.

Description

Microbial combination capable of effectively inhibiting odor production of biomass fermentation from source and application thereof

Technical Field

The invention belongs to the technical field of gas purification, and particularly relates to a functional complementary odor-inhibiting strain combination and application thereof.

Background

The mass production of malodorous gases seriously affects the living environment of residents and endangers the physical health of human beings. The livestock manure and kitchen waste are two types of biomass which are easy to decompose and produce odor, the daily discharge amount of the livestock manure in China reaches 700 ten thousand tons at present, the annual production total amount is about 24 hundred million tons, and the production amount of the excrement is about 16 hundred million tons and 8 hundred million tons respectively; in the urban household garbage structure of China, the kitchen garbage accounts for 30% -50%, the generation amount of the urban kitchen garbage is not less than 6000 ten thousand tons every year, and the generation amount also shows a continuously increasing trend. The main components of the livestock manure and kitchen garbage are organic matters such as protein, sugar, fat and the like, wherein the organic matters such as protein, amino acid and the like can perform decarboxylation and deamination due to the activity of microorganisms, so that a large amount of malodorous gas is generated, and the air quality and the body health of surrounding residents are seriously influenced; thus, the treatment of the odor generated by the decomposition of the biomass is not slow.

At present, the odor is mainly treated by adopting methods such as chemical absorption, oxidation, physical absorption, shielding and the like, so that the problems of secondary pollution, temporary treatment, and the like exist, and the resources in the biomass are partially consumed as the components in the biomass are converted into the odor; aiming at the problems, development of a microbial agent capable of effectively inhibiting the generation of livestock manure and kitchen waste odor from the source is needed to meet the national green development requirement.

Disclosure of Invention

Aiming at the problem that the existing biomass decomposition odor production is difficult to treat, the invention discloses a microbial combination for effectively inhibiting the biomass fermentation odor production from the source and application thereof, and the beneficial microbial source is utilized to inhibit the odor production, so that the microbial combination has the advantages of simple method, excellent effect, contribution to preserving nutrient components in biomass and the like.

The technical scheme of the invention is as follows:

the invention provides a deodorizing microorganism combination, which comprises one or more of saccharomycetes, decomposed bacteria or nitrifying bacteria; when the three are combined, the mass ratio of the saccharomycetes to the decomposed bacteria to the nitrifying bacteria is (1-3) to (1-3).

The microzyme of the invention is Saccharomyces cerevisiae NAU-OR3, is classified and named Saccharomyces cerevisiae, and is preserved in the microorganism strain collection of Guangdong province at the 8 th month 18 of 2021, and the address is: building 5, no. 59, of Guangzhou Md.A. Xian Zhonglu No. 100, accession number GDMCC No. 61877.

The decomposed bacteria are Bacillus licheniformis NAU-OR5, are classified and named Bacillus licheniformis, and are preserved in the microorganism strain collection of Guangdong province at the 8 th month 18 of 2021, and are addressed: building 5, no. 59, of Guangzhou Md.A. Xian Zhonglu No. 100, accession number GDMCC No. 61879.

The nitrifying bacteria are Paracoccus denitrificans NAU-OR1, are classified and named Paracoccus denitrificans, and are preserved in the microorganism strain collection of Guangdong province at the date of 28 of 2021, and are addressed: building 5, no. 59 of Guangzhou Mr. Xian Zhonglu 100, and the preservation number is GDMCC No. 61875).

In some embodiments, the mass ratio of the saccharomycetes, the decomposed bacteria and the nitrifying bacteria is (1-2): 1-3; in some more specific examples, the mass ratio of the yeast, the decomposed bacteria and the nitrifying bacteria is 1 (2-3): 1-3.

The invention also provides a preparation method of the odor-inhibiting microorganism combination, which comprises the steps of mixing strains with a certain proportion; the preparation method of each strain may include the following steps in a specific example, according to a conventional method in the art: respectively activating each strain stored at-80 ℃ on a flat culture medium, then picking a single colony, culturing in a liquid culture medium to a logarithmic phase, transferring into a seed culture medium, and performing gradual expansion culture; and centrifugally collecting thalli and spores. During the expansion culture, components favorable for the spore production of the thalli can be added.

The invention also provides an odor-inhibiting microbial agent, which comprises the odor-inhibiting microbial combination and organic silicon, wherein the weight ratio of the odor-inhibiting microbial combination to the organic silicon is (10-50): 1, preferably 10:1.

the invention also provides an application of the odor-inhibiting microorganism combination or the odor-inhibiting microbial agent in treating the biomass producing malodor by fermentation.

The fermented malodorous biomass can be any substance capable of generating gases such as ammonia gas, hydrogen sulfide, mercaptan, thioether, volatile organic compounds, comprehensive malodor and the like, and in a specific example, the fermented malodorous biomass is livestock manure or kitchen waste.

In one example of the invention, when treating and inhibiting kitchen waste, the mass ratio of the saccharomycetes, the decomposed bacteria and the nitrifying bacteria is 1:2:1.

In one example of the invention, when the livestock manure is inhibited, the mass ratio of the saccharomycetes, the decomposed bacteria and the nitrifying bacteria is 1:3:2.

In some examples of the invention, when the malodorous biomass produced by fermentation is kitchen waste, the amount of the combination of malodorous inhibiting microorganisms is more than 3% by weight, preferably between 3% and 10% by weight, and most preferably 3% by weight, based on the dry weight of the kitchen waste.

In some examples of the invention, when the malodorous biomass produced by fermentation is livestock manure, the combined amount of the malodorous-inhibiting microorganisms is more than 6% by weight, preferably 8% to 10% by weight, and most preferably 8% by weight of the dry basis of the livestock manure.

The invention also provides a use method of the odor-inhibiting microbial agent, wherein the odor-inhibiting microbial agent is prepared into a bacterial liquid by mixing the odor-inhibiting microbial agent and water according to a ratio of 1:100, and the organic silicon with a formula amount is added. The prepared deodorant can be directly sprayed to biomass.

The invention provides a novel biological deodorant prepared by fermenting and compounding microorganism strains, wherein active ingredients of the microorganism combination are saccharomycetes, decomposed bacteria and nitrifying bacteria derived from kitchen waste or livestock manure. The screened microorganisms come from the production living biomass waste, so that the biological safety of the living biomass waste can be ensured; the odor inhibiting microbial inoculum can convert nutritional ingredients in biomass, so that the generation of malodorous gases such as ammonia, hydrogen sulfide, methyl mercaptan, volatile organic compounds and the like is inhibited from the source, and the environment-friendly and emission-reducing concept is responded well. The odor-inhibiting microorganism combination can be widely applied to places with odor such as livestock farms, garbage transfer stations, garbage disposal sites, zoos, sewage treatment plants, sewage pools (ditches), toilets, restaurants and the like.

In the invention, the odor-inhibiting microorganism combination relates to a microorganism 'filtering' process when inhibiting the odor production of biomass, and the odor-related substances are converted or the generated odor is degraded through the metabolic activity of the microorganism based on the absorption of metabolites and the fixed conversion of the microorganism, and the specific process is as follows: (1) The decomposed microbial inoculum is used as a dominant strain to degrade macromolecules such as starch, cellulose, protein and the like in biomass into micromolecules; (2) Part of the generated odor is dissolved in the odor inhibiting bacteria agent and is changed from gas phase to liquid phase; (3) The nitrifying bacteria can convert the components which are easy to generate the odor into nitrate or nitrite, so that the biomass is degraded and converted to inhibit the odor. The conversion of malodorous substances is the speed limiting stage of the process, and after the microorganisms degrade biomass, the biomass is used as the nutrients of other microorganisms to continue to absorb and digest, so that the malodorous substances are gradually converted and degraded by circulation.

The use method of the odor-inhibiting microorganism combination provided by the invention not only can greatly improve the action effect, but also can furthest reduce the treatment cost, and is beneficial to large-area popularization and application.

The microorganism combination has the capability of inhibiting the generation of comprehensive malodor reaching more than 99 percent; the addition of the organic silicon can obviously reduce the contact angle of the bacterial liquid on the biomass from 53.75 degrees to 23.73 degrees, greatly improve the contact area of the bacterial agent and the biomass, and further improve the effect of inhibiting the odor.

Drawings

FIG. 1 shows the removal of odor components by 3 strains of microorganisms;

FIG. 2 shows the influence of different proportions on kitchen waste odor;

FIG. 3 shows the influence of different proportions on the odor of the livestock and poultry manure;

FIG. 4 shows the effect of different access amounts on kitchen waste odor;

FIG. 5 shows the effect of different amounts of intake on the odor of livestock and poultry feces;

FIG. 6 shows the effect of different amounts of adjuvant on the contact angle of the microbial inoculum.

Detailed Description

The following examples are provided to illustrate the combination of microorganisms and methods of use of the invention for efficiently inhibiting the production of malodor by fermentation of biomass at a source, but are not to be construed as limiting the scope of the invention. The experimental methods in the following examples are conventional methods unless otherwise specified. The test materials used in the examples described below, unless otherwise specified, were purchased from conventional biochemical reagent stores.

According to the invention, the concentration of ammonia gas after kitchen waste fermentation can be rapidly increased in a short period, and the peak value is reached at 24 hours; the comprehensive odor and methyl mercaptan reach peak values at 72 hours, and have descending trend after reaching the peak values; the comprehensive odor, hydrogen sulfide, methyl mercaptan and volatile organic compounds of the fermented livestock and poultry manure reach peak values in 48 hours, and the ammonia gas reaches peak values in 96 hours; the odor yield of the livestock manure is about 4 times of that of kitchen waste. In the following examples, these two types of biomass were selected for experiments, and it should be specifically noted that the application of the present invention is not limited to these two types of malodorous biomass, and similar substances capable of generating gases such as ammonia, hydrogen sulfide, mercaptans and sulfides, volatile organic compounds and comprehensive malodors can achieve similar effects.

The pig manure used in the examples below was taken from a modern farm of gao, unless otherwise specified, and the initial yields of ammonia, hydrogen sulfide, methyl mercaptan, volatile organics and comprehensive malodour were 18.08ppm/g, 2.19ppm/g, 0.29ppm/g, 1.26ppm/g dry manure and 690.15/g dry manure, respectively (dimensionless, in terms of per gram dry manure, the same applies below). The initial yields of the kitchen waste from the ammonia gas, hydrogen sulfide, methyl mercaptan, volatile organic compounds and comprehensive malodor in the canteen of the Pukou school district of Nanjing agricultural university are 3.66ppm/g, 0.03ppm/g, 1.98ppm/g, 1.72ppm/g dry waste and 97.10/g dry waste respectively.

Unless otherwise indicated, the media used in the examples below were as follows:

the liquid culture medium and the seed culture medium of the saccharomycetes are prepared from the following components: 10g/L of yeast extract, 20g/L of bactopeptone, 20g/L of agar, 200g/L of potato and 20g/L of glucose;

the ratio of the liquid culture medium and the seed culture medium of the decomposed bacteria is as follows: 10g/L tryptone, 5g/L yeast extract, 5g/L sodium chloride and 1N sodium hydroxide 1mL/L;

the liquid culture medium and the seed culture medium of nitrifying bacteria are prepared from the following components: 5g/L of ammonium sulfate, 0.5g/L of magnesium sulfate, 0.7g/L of monopotassium phosphate and 0.5g/L of calcium chloride.

EXAMPLE 1 isolation, identification and preservation of three strains

The saccharomycete is separated from kitchen waste, 10.0g (wet weight) of the kitchen waste is added into 20mL of water and stirred uniformly; after a little precipitation, 100 mu L of supernatant is coated on YPD culture medium added with 100mg/mL penicillin and levofloxacin, after the strain grows out, elliptic uplift colony is selected for purification culture, functional strain sample feeding and sequencing are carried out through functional verification, and the ITS sequence is shown as SEQ ID NO. 1. After comparison, the strain is determined to be Saccharomyces cerevisiae (Saccharomyces cerevisiae) NAU-OR3, classified and named Saccharomyces cerevisiae, and the strain is preserved in the microorganism strain collection of Guangdong province at 18 days of 2021, with the preservation number of: GDMCC 61877.

The decomposed bacteria are separated from the primarily fermented livestock manure, and 10.0g (wet weight) of the primarily fermented livestock manure is added into 20mL of water and stirred uniformly; after a little precipitation, 100 mu L of supernatant is coated on an LB culture medium plate, after the supernatant grows out, single colony is selected for purification culture, functional strains are sequenced through functional verification, and the 16sRNA sequence is shown as SEQ ID NO. 2. The strain was identified as Bacillus licheniformis (Bacillus licheniformis) NAU-OR5 by comparison, classified under the name Bacillus licheniformis, and deposited at the Cantonese microorganism strain collection at 18/8 of 2021 under the accession number: GDMCC 61879.

The nitrifying bacteria are separated from the primarily fermented livestock and poultry manure, 10.0g (wet weight) of the primarily fermented livestock and poultry manure is added into 20mL of water, and the mixture is stirred uniformly; after a little precipitation, 100 mu L of supernatant is coated on a nitrifying bacteria culture medium plate, after the supernatant grows out, single colony is selected for purification culture, and functional strains are sequenced after functional verification, wherein the 16sRNA sequence is shown as SEQ ID NO. 3. After comparison, the strain is determined to be Paracoccus denitrificans (Paracoccus denitrificans) NAU-OR1, classified and named Paracoccus denitrificans, and is preserved in the microorganism strain collection of Guangdong province at 10 and 28 days of 2021, with the preservation number: GDMCC 61875.

EXAMPLE 2 preparation of deodorant microorganism combinations

The preparation method of each strain comprises the following steps: activating the strain stored at-80 ℃ on a flat culture medium, picking a single colony, culturing in a liquid culture medium to a logarithmic phase, transferring into a seed culture medium, and performing expansion culture step by step; centrifugally collecting thalli and spores; adding components beneficial to spore production of the thalli during the expansion culture.

According to the method, after the strains of saccharomycetes, decomposed bacteria and nitrifying bacteria are respectively activated on a solid culture medium, single bacterial colonies are selected and inoculated into 2mL of corresponding liquid culture medium, after the culture is carried out to a logarithmic phase, the liquid culture medium is transferred into a seed culture medium (the ratio is 1:1000), and the liquid culture medium is subjected to gradual expansion culture; and centrifugally collecting thalli and spores. The activated solid culture medium of the saccharomycetes is as follows: 10g/L of yeast extract, 20g/L of bactopeptone, 20g/L of agar, 200g/L of potato, 20g/L of glucose and 18g/L of agar; the activated solid culture medium of the decomposed bacteria is as follows: 10g/L of tryptone, 5g/L of yeast extract, 5g/L of sodium chloride, 1mL/L of 1N sodium hydroxide and 15g/L of agar; the solid culture medium for activating nitrifying bacteria is as follows: 5g/L of ammonium sulfate, 0.5g/L of magnesium sulfate, 0.7g/L of monopotassium phosphate, 0.5g/L of calcium chloride and 18g/L of agar; when the microbial inoculum is produced in large tanks, the carbon source and the nitrogen source are required to be adjusted into starch, soybean powder, amylase and the like (can be adjusted according to the conventional method in the field), and an antifoaming agent is added; to promote sporulation of the cells, an amount of molasses is added (the amount added may be adjusted according to a conventional method in the art). The culture temperature of the strain is 30-35 ℃. And centrifugally collecting thalli and spores to obtain corresponding strains.

The strains are mixed according to the proportion to form the odor-inhibiting microorganism combination, and the odor-inhibiting microorganism combination can be directly mixed with water according to the ratio of 1:100 to prepare bacterial liquid, and the bacterial liquid is sprayed on biomass.

The embodiment also provides a preparation and use method of the odor inhibiting microbial agent, which comprises the steps of preparing a microbial liquid by the prepared odor inhibiting microbial combination and water according to a ratio of 1:100, adding organic silicon, and then spraying the microbial liquid on biomass.

In the invention, under the condition of not spraying any microbial inoculum, the odor components and the yields of different biomass materials are different, wherein the yields of ammonia gas, hydrogen sulfide, methyl mercaptan, volatile organic compounds and comprehensive malodors of pig manure are respectively 18.08ppm/g, 2.19ppm/g, 0.29ppm/g, 1.26ppm/g dry manure and 690.15/g dry manure (dimensionless, the following is the same according to each gram of dry manure); the Saccharomyces cerevisiae sprayed alone has strong capability of fixing and converting nitrogen, and the yield of ammonia is only about 5%; bacillus licheniformis has the capability of inhibiting the generation of methyl mercaptan, hydrogen sulfide, comprehensive malodor and ammonia of 100%, 99.29%, 98.93% and 85.81% respectively, but has weaker capability of inhibiting the generation of volatile organic compounds; the nitrifying bacteria have relatively uniform capability of inhibiting the generation of odor, and the capability of inhibiting ammonia, hydrogen sulfide, methyl mercaptan, volatile organic compounds and comprehensive malodor are respectively 80.25%, 82.80%, 79.02%, 30.43% and 82.97%; when 3 strains are compounded according to the ratio of 1:2:1 or 1:3:2, the deodorization capacity is larger than that of simple superposition of three strains, so that the selected combined microorganisms have the function complementation and synergistic effect, and have extremely strong capacity of inhibiting the decomposition of biomass and the odor production.

EXAMPLE 3 odor control Effect of microorganism combinations

1. Materials and methods

The instrumentation used is shown in table 1.

Table 1 instrument and apparatus

Chemical reagent: potassium sulfate and glucose were purchased from national pharmaceutical chemicals, inc, all analytically pure; copper sulfate was analytically pure and purchased from ala Ding Shiji limited. Pig manure was obtained from Rugao Huari modern farm and kitchen waste was obtained from Nanjing university Pukou school canteen.

2. Test method

2.1 cell collection of cells

Activating according to the method described in example 2, picking single colony of activated saccharomycetes, decomposed bacteria and nitrifying bacteria, inoculating to 2mL of corresponding liquid culture medium, placing in an incubator at 30 ℃, and shaking for 120r/min overnight culture; 300 mu L of the culture solution is inoculated into 300mL of liquid culture medium, 160r/min is used for culturing to logarithmic phase, yeast and decomposed bacteria are cultured for 6 hours, and nitrifying bacteria are cultured for 24 hours. And centrifuging for 10min under 10000r/min to collect thalli.

2.2 determination of odor inhibiting Effect

Preparing bacterial liquid from the bacterial cells collected in 2.1 and water according to a ratio of 1:100, and testing the capability of 3 bacterial agents for inhibiting the generation of ammonia, hydrogen sulfide, mercaptan, thioether, volatile organic compounds and comprehensive malodor by taking water as a reference. The calculation formula of the inhibition ratio (Y): y= (Y water-Y microbial inoculum)/Y water x 100%

2.3 experiments on the combined effects of microorganisms

Preparing saccharomycetes, decomposed bacteria and nitrifying bacteria collected in 2.1 into bacterial liquid according to the mass ratio of 3:1:1, 2:2:1, 1:1:1, 1:2:1, 1:3:1, 1:3:2 and 1:3:3, spraying the bacterial liquid onto 5kg kitchen waste or livestock manure according to the amounts of 3 per mill and 8 per mill respectively, and measuring the reduction condition of various odor indexes after 5 hours.

2.4 Effect experiment of deodorant inoculum size

Weighing deodorant bacteria agent by 1%o, 2%o, 3%o, 6%o, 8%o and 10%o of biomass dry basis, preparing bacterial liquid according to a ratio of 1:100, spraying onto 5kg kitchen waste or livestock and poultry manure, and measuring reduction conditions of various odor indexes after 5 hours.

2.5 experiment of the Effect of adjuvants

Selecting triton, pure ester and organic silicon as auxiliary agents, and detecting survival and contact angle change conditions of saccharomycetes, decomposed bacteria and nitrifying bacteria after adding 1 per mill of the auxiliary agents; and 0.5 per mill, 1 per mill, 3 per mill and 5 per mill of the bacterial liquid are added with auxiliary agents to measure the change condition of the contact angle.

2.6 data processing and analysis

The resulting data were statistically analyzed using IBM SPSS Statistics, excel 2016, origin 2018 software; the difference in significance between the different treatments was examined using Duncan, α=0.05.

3 results of experiments

3.1 deodorization Effect of the three microorganisms of the present invention

The research selects 4 microorganism strains, and takes chemical deodorizing reagent (provided by the university of ataxia) and deionized water as control to compare the deodorizing effect, as shown in figure 1, the result shows that the efficiency of inhibiting the generation of ammonia gas, hydrogen sulfide, methyl mercaptan and comprehensive malodor of the bacillus licheniformis disclosed by the invention reaches 85.81%, 99.29%, 100.00% and 98.93%, respectively, wherein the inhibiting capability on hydrogen sulfide and comprehensive malodor is obviously higher than that of other treatments (P < 0.05); the production of volatile organic compounds (eVOC) can not be greatly inhibited by each treatment, but the nitrifying bacteria and the saccharomycetes respectively reach 30.43 percent and 27.70 percent, which are obviously higher than those of other treatments (P is less than 0.05), so that the nitrifying bacteria, the saccharomycetes and the decomposed bacteria have good malodor removing effect.

3.2 deodorization effect of microorganisms in different ratios

The yeast, the rotten bacteria and the nitrifying bacteria are prepared into bacterial liquid according to the mass ratio of 3:1:1, 2:2:1, 1:1:1, 1:2:1, 1:3:1, 1:3:2 and 1:3, and sprayed onto kitchen waste and livestock manure according to the amounts of 3 per mill and 8 per mill respectively, and the result shows that the rotten bacteria, the nitrifying bacteria and the yeast are compounded into the odor-inhibiting microorganism combination, which can make up the defects of each bacterial strain in terms of inhibiting the generation of volatile organic matters and exert the advantages of the Bacillus licheniformis in terms of inhibiting the generation of ammonia, hydrogen sulfide, mercaptan, thioether and comprehensive malodor, has remarkable deodorizing effect, has remarkable odor-inhibiting effect in the preferred range of the invention, and particularly when the ratio is 1:2:1, the ammonia gas, hydrogen sulfide and comprehensive malodor produced by kitchen waste fermentation are obviously inhibited (P < 0.05), and the malodor concentration is reduced by more than 90% without adding bacteria; the odor of the livestock manure can be obviously inhibited when the ratio is 1:3:2 (figures 2 and 3).

3.3 inhibition of different microbial Access to the odor production from Biomass fermentation

The odor inhibiting bacteria agent is weighed by 1 per mill, 2 per mill, 3 per mill, 6 per mill, 8 per mill and 10 per mill of dry basis of kitchen waste and livestock manure, and is sprayed onto the kitchen waste and the livestock manure after being prepared into bacterial liquid according to a ratio of 1:100, and tests prove that the odor inhibiting bacteria agent has obvious odor inhibiting effect within the range of the access amount of the invention, particularly the effect of the bacteria agent reaching 3 per mill and more than that of the kitchen waste is obviously superior to that of the bacteria agent reaching 1 per mill and 2 per mill (P < 0.05), and the effect of the bacteria agent reaching 6 per mill and more than that of the livestock manure is obvious (P > 0.05), and the 3 per mill and 8 per mill of the bacteria agent are respectively the optimal access amount of the kitchen waste and the livestock manure from the economical point of view (figures 4 and 5).

3.4 effects of adjuvants on the combination of odor-inhibiting microorganisms

After the auxiliary agents such as triton, pure ester or organic silicon are added into the microorganism combination, the bacterial number is found to be not obviously reduced (P is more than 0.05) compared with that of the bacteria added with sterile water through plate counting; further comparing the contact angle changes of different auxiliary agents and the auxiliary agents without adding the auxiliary agents, the contact angle is reduced from 53.75 degrees to 23.73 degrees after only the organic silicon is added, so that the infiltration of the microbial inoculum on the surface of the garbage is increased; and the addition amount of the auxiliary agent of the invention can greatly reduce the contact angle by the effect of 0.5%o, 1%o, 3%o, 5%o and the like, and the addition amount of the auxiliary agent of the invention of 1%o is optimal from the economical point of view because the effect of more than 1%o is increased and relaxed (figure 6).

Example 4 inhibition of sheep manure malodor

As reflected by the modern Rugao Huari farm, the produced sheep manure is difficult to treat and has large odor; through detection, the comprehensive malodor reaches 658.78/gram of dry feces; the microbial combination prepared under the conditions described in the example 2 of the manure of the farm is that microzyme, decomposed bacteria and nitrifying bacteria are compounded according to a ratio of 1:3:2, 1%o of organic silicon auxiliary agent is added, spraying is carried out according to 8%o of dry weight of the sheep manure, and the comprehensive malodor index is reduced by 92.6% compared with the initial value after 5 hours.

Example 5 inhibition of odor of canteen leftovers

In this test, three plastic bottles (numbered #1, #2, and # 3) of the same volume were filled with 2kg of remaining meal in a mixing canteen, and the mouth was closed to allow the bottles to ferment at 35℃and accumulate odor.

Opening two bottles #1 and #2 after half a month, wherein malodorous smell exists in the two bottles; spraying 3 per mill (based on initial dry weight) of the microorganism combination using the conditions described in example 2 into a bottle 1, namely adding 1 per mill of an organosilicon auxiliary agent after yeast, decomposed bacteria and nitrifying bacteria are compounded according to a ratio of 1:2:1; at the next 24 hours, the smell of both bottles changed: the odor in the bottle 1 sprayed with the odor inhibitor is obviously lightened, and the odor in the bottle 2 is not obviously changed; the #1, #2 bottles were capped and placed on hold.

After one month, three bottles were opened and the odor of #1, #2, #3 was compared. The odor indexes of ammonia gas, hydrogen sulfide, mercaptan, thioether, volatile organic compounds, comprehensive malodor and the like in the bottle #1 are obviously lower than those of the two bottles #2 and #3, and no obvious difference exists between the odor indexes of the two bottles #2 and # 3. Through the experiment, the microorganism combination has obvious inhibition effect on the generation of odor and obvious effect.

The foregoing is merely a preferred embodiment of the present invention and it should be noted that modifications and adaptations to those skilled in the art may be made without departing from the principles of the present invention, which are intended to be comprehended within the scope of the present invention.

Sequence listing

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tgtcgtgaga tgttgggtta agtcccgcaa cgagcgcaac ccttgatctt agttgccagc 1080

attcagttgg gcactctaag gtgactgccg gtgacaaacc ggaggaaggt ggggatgacg 1140

tcaaatcatc atgcccctta tgacctgggc tacacacgtg ctacaatggg cagaacaaag 1200

ggcagcgaag ccgcgaggct aagccaatcc cacaaatctg ttctcagttc ggatcgcagt 1260

ctgcaactcg actgcgtgaa gctggaatcg ctagtaatcg cggatcagca tgccgcggtg 1320

aatacgttcc cgggccttgt acacaccgcc cgtcacacca cgagagtttg taac 1374

<210> 3

<211> 1285

<212> DNA

<213> Paracoccus denitrificans (Paracoccus denitrificans)

<400> 3

aacccttggg ggttagcggc ggacgggtga gtaacgcgtg ggaatatgcc ctttgctacg 60

gaatagcccc gggagactgg gattaatacc gtatacgccc tttgggggag agatttatcg 120

gcaaaggatt agcccgcgtt ggattaggta gttggcgggg taatggccta ccaagccgac 180

gatccatagc tggtttgaga ggatgatcac ccacactggg actgagacac ggcccagact 240

cctacgggag gcagtggggg ggaatcttag acaatggggg caaccctgat ctagccatgc 300

cgcgtgagtg atgaaggccc tagggttgta aagctctttc agctgggaag ataatgacgg 360

taccagcaaa agaagccccg gctaactccg tgccagcagc cgcggtaata cggagggggc 420

tagcgttgtt cggaattact gggcgtaaag cgcacgtagg cggaccggaa agttgggggt 480

gaaatcccgg ggctcaacct cggaactgcc ttcaaaacta tcggtctgga gttcgagaga 540

ggtgagtgga attccgagtg tagaggtgaa attcgtagat attcggagga acaccagtgg 600

cgaaggcggc tcactggctc gatactgacg ctgaggtgcg aaagcgtggg gagcaaacag 660

gattagatac cctggtagtc cacgccgtaa acgatgaatg ccagtcgtcg ggcagcatgc 720

cgttcggtga cacacctaac ggattaagca ttccgcctgg ggagtacggt cgcaagatta 780

aaactcaaag gaattggcgg ggggccgcac aagcggtgga gcatgtggtt taattcgaag 840

ctacgcgcag aaccttacca tcccttgaca tcgcaggccc gctccagaga tggagttttg 900

ttgtaagagg cctgtggtca ggtggtgcat ggctgtcgtc agctcgtgtc gtgagatgtt 960

cggttaagtc ctgcaacgag cgcacccccc actgttagtt gccagcattt ggttgggcac 1020

tataagagaa ctgccgatga taagtcggag gaaggtgtgg atgacgtcaa gtcctcatgg 1080

cccttacggg ttgggctaca cacgtgctac aatggtggtg acagtgggtt aatccccaaa 1140

agccatctca gttcggattg gggtctgcaa ctcgacccca tgaagttgga atcgctagta 1200

atcgcggaac agcatgccgc ggtgaatacg ttcccgggcc ctgtacacac cccccgtcac 1260

accacgggag ttgggtctac cccac 1285

Claims

1. The odor-inhibiting microorganism combination is characterized by comprising yeast, decomposed bacteria and nitrifying bacteria, wherein the mass ratio of the yeast to the decomposed bacteria to the nitrifying bacteria is (1-3): 1-3: (1-3); the yeast isSaccharomyces cerevisiaeNAU-OR3 with a preservation number of GDMCC No. 61877 and a rotten strainBacillus licheniformisNAU-OR5 with accession number GDMCC No. 61879 and nitrifying bacteriaParacoccus denitrificansNAU-OR1, accession number GDMCC No. 61875.

2. The odor-inhibiting microorganism combination of claim 1, wherein the mass ratio of the yeast, the decomposed bacteria and the nitrifying bacteria is (1-2): 1-3.

3. The odor-inhibiting microorganism combination of claim 2, wherein the mass ratio of the yeast, the decomposed bacteria and the nitrifying bacteria is 1 (2-3): 1-3.

4. The method for producing a deodorizing microorganism combination according to any one of claims 1 to 3, wherein the strains are mixed in a specific amount.

5. An odor-inhibiting microbial agent, which is characterized by comprising the odor-inhibiting microbial composition and organic silicon according to any one of claims 1 to 3, wherein the weight ratio of the odor-inhibiting microbial composition to the organic silicon is (10-50): 1.

6. the odor control microbial agent of claim 5 wherein the weight ratio of odor control microbial combination to silicone is 10:1.

7. use of a combination of odour-inhibiting microorganisms according to any one of claims 1 to 3 or a odour-inhibiting bacterial agent according to claim 5 or 6 for the treatment of a fermented malodour-producing biomass.

8. The use according to claim 7, wherein the fermented malodorous biomass is any substance capable of producing one or more of ammonia, hydrogen sulfide, mercaptans, sulfides, volatile organics or complex malodorous gases.

9. The use according to claim 8, wherein the malodorous biomass produced by fermentation is livestock manure or kitchen waste.

10. The use according to claim 8, wherein the mass ratio of the yeast, the decomposing bacteria and the nitrifying bacteria is 1:2:1 when the kitchen waste is treated.

11. The use according to claim 8, wherein the mass ratio of the yeast, the decomposing bacteria and the nitrifying bacteria is 1:3:2 when the livestock manure is treated.

12. The use according to claim 8, wherein when the malodorous biomass produced by fermentation is kitchen waste, the combined use amount of the malodorous-inhibiting microorganisms is more than 3% by weight of the dry basis of kitchen waste.

13. The use according to claim 12, characterized in that when the malodorous biomass produced by fermentation is kitchen waste, the combined use of malodorous-inhibiting microorganisms is 3-10% by weight of the dry basis of kitchen waste.

14. The use according to claim 13, characterized in that when the malodorous biomass produced by fermentation is kitchen waste, the combined use of malodorous-inhibiting microorganisms is 3% by weight of the dry basis of kitchen waste.

15. The use according to claim 8, wherein when the malodorous biomass produced by fermentation is livestock manure, the combined use amount of the malodorous inhibiting microorganisms is more than 6 per mill based on the dry weight of the livestock manure.

16. The use according to claim 15, wherein when the malodorous biomass produced by fermentation is livestock manure, the combined use amount of the malodorous inhibiting microorganisms is 8 to 10 per mill based on the dry weight of the livestock manure.

17. The use according to claim 16, wherein when the malodorous biomass produced by the fermentation is livestock manure, the combined use amount of the malodorous inhibiting microorganisms is 8 per mill based on the dry weight of the livestock manure.