CN110117567A - A kind of Paracoccus denitrificans bacterial strain screening and its application in deodorization - Google Patents
A kind of Paracoccus denitrificans bacterial strain screening and its application in deodorization Download PDFInfo
- Publication number
- CN110117567A CN110117567A CN201910479735.XA CN201910479735A CN110117567A CN 110117567 A CN110117567 A CN 110117567A CN 201910479735 A CN201910479735 A CN 201910479735A CN 110117567 A CN110117567 A CN 110117567A
- Authority
- CN
- China
- Prior art keywords
- paracoccus denitrificans
- ammonia
- application
- paracoccus
- denitrificans
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
Classifications
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01D—SEPARATION
- B01D53/00—Separation of gases or vapours; Recovering vapours of volatile solvents from gases; Chemical or biological purification of waste gases, e.g. engine exhaust gases, smoke, fumes, flue gases, aerosols
- B01D53/34—Chemical or biological purification of waste gases
- B01D53/74—General processes for purification of waste gases; Apparatus or devices specially adapted therefor
- B01D53/84—Biological processes
-
- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F3/00—Biological treatment of water, waste water, or sewage
- C02F3/34—Biological treatment of water, waste water, or sewage characterised by the microorganisms used
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/20—Bacteria; Culture media therefor
- C12N1/205—Bacterial isolates
-
- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F2101/00—Nature of the contaminant
- C02F2101/10—Inorganic compounds
- C02F2101/101—Sulfur compounds
-
- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F2101/00—Nature of the contaminant
- C02F2101/10—Inorganic compounds
- C02F2101/16—Nitrogen compounds, e.g. ammonia
-
- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F2101/00—Nature of the contaminant
- C02F2101/10—Inorganic compounds
- C02F2101/16—Nitrogen compounds, e.g. ammonia
- C02F2101/163—Nitrates
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12R—INDEXING SCHEME ASSOCIATED WITH SUBCLASSES C12C - C12Q, RELATING TO MICROORGANISMS
- C12R2001/00—Microorganisms ; Processes using microorganisms
- C12R2001/01—Bacteria or Actinomycetales ; using bacteria or Actinomycetales
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A50/00—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE in human health protection, e.g. against extreme weather
- Y02A50/20—Air quality improvement or preservation, e.g. vehicle emission control or emission reduction by using catalytic converters
Landscapes
- Engineering & Computer Science (AREA)
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Health & Medical Sciences (AREA)
- Organic Chemistry (AREA)
- Microbiology (AREA)
- Biomedical Technology (AREA)
- Environmental & Geological Engineering (AREA)
- Genetics & Genomics (AREA)
- Biotechnology (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Medicinal Chemistry (AREA)
- Analytical Chemistry (AREA)
- Chemical Kinetics & Catalysis (AREA)
- General Chemical & Material Sciences (AREA)
- Water Supply & Treatment (AREA)
- Tropical Medicine & Parasitology (AREA)
- Virology (AREA)
- Oil, Petroleum & Natural Gas (AREA)
- Biodiversity & Conservation Biology (AREA)
- Molecular Biology (AREA)
- Hydrology & Water Resources (AREA)
- Biochemistry (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
Abstract
The invention belongs to microorganisms technical fields, and in particular to a kind of Paracoccus denitrificans, strain name: Paracoccus denitrificans, Paracoccus denitrificans TS-1；Deposit number: CGMCC NO.17605.Paracoccus denitrificans of the invention has the 16SrDNA sequence as shown in SEQ ID No.1.Meanwhile the application the invention also discloses Paracoccus denitrificans TS-1 in deodorization and sewage treatment.Paracoccus denitrificans TS-1 of the invention not only heterotrophic nitrification with higher, aerobic denitrification ability, while also there is the stronger ability for removing sulphide removal, be one plant simultaneously there are three types of tools function bacterial strain.
Description
Technical field
The invention belongs to microorganisms technical fields, and in particular to a kind of Paracoccus denitrificans bacterial strain screening and its in deodorization
Using.
Background technique
Odorant pollutant refers to that all stimulations olfactory organoleptic causes people unhappy and the gaseous matter of damage living environment,
Its substance classes is various, and coverage is big, administers the attention for also gradually obtaining people.Exist in fowl and animal excrement and house refuse
A large amount of deodorizing microorganism can generate a large amount of ammonia and hydrogen sulfide in garbage loading embeading and composting process, not only to the body of people
Body causes to seriously endanger, also pollution environment, polluted underground water source etc..
Conventional deodorizing methods has at present: physical deodorization method, chemical deodorization method, biological odor removal method.Physical deodorization method be according to
Foul odour is eliminated in conversion between solid, liquid, gas three-phase, only reduces smell to the perception degree of smell, however its
Chemical property does not have radical change.Chemical deodorization method is the certain chemical reagent of addition, changes its chemical structure to destroy its deodorization
Group is changed into odorless or the lower substance of stink.Bioanalysis is a kind of novel Deodor method developed in recent years,
Because investment is low with operating cost, treatment effeciency is high, does not generate the advantages that secondary pollution for it, the mainstream side of deodorization is just gradually developed into
Method.
Bioanalysis includes zinc cation method, biological filter process and biological island.Compared with other two methods, biology drop
Filter tower accurately controls reaction condition (such as humidity, pH), it will be apparent that improves removal efficiency.Composting plant, refuse landfill,
In farm's deodorisation process, high-effect bacterial is linked into deodorization reactor, foul smell can be quickly removed, accomplish free from extraneous odour,
It is without secondary pollution etc., it is compost deodorizing, the preferable selection of the depollutions of environment such as refuse landfill can be with wide popularization and application.
Summary of the invention
The object of the present invention is to provide one plant to have good drop with the Paracoccus denitrificans of efficient denitrification desulfurization, the bacterial strain
Solve the ability of ammonia nitrogen, nitrate nitrogen and sulfide.It cannot be only used for waste water of the degradation treatment containing ammonia nitrogen, nitrate nitrogen and inorganic sulphide, together
When be applicable to trickled-bed biofilter for handling the toxic and harmful gas such as ammonia, hydrogen sulfide.
For achieving the above object, the technical scheme adopted by the invention is that:
A kind of Paracoccus denitrificans, strain name: Paracoccus denitrificans, Paracoccus denitrificans TS-1, preservation
Number: CGMCC NO.17605.
Preferably, the 16SrDNA sequence of the Paracoccus denitrificans TS-1 is as shown in SEQ ID No.1.
Preferably, application of the Paracoccus denitrificans in deodorization.
Preferably, application of the Paracoccus denitrificans in deodorization, specific steps are as follows:
(1) Paracoccus denitrificans TS-1 is cultivated as Paracoccus denitrificans TS-1 bacterium solution, Paracoccus denitrificans TS-1 in the bacterium solution
Viable bacteria amount be 108~109CFU/mL；
(2) solution for providing required nutrition for Paracoccus denitrificans TS-1 growth is added to the bio-trickling filter that filler is housed
In, step (1) resulting Paracoccus denitrificans TS-1 bacterium solution is then added；
(3) with the air velocity of 4~6L/min, 40~60mg/m of gas concentration3Hydrogen sulfide air inflow and gas concentration
40~60mg/m3Ammonia air inflow into bio-trickling filter simultaneously be passed through air, hydrogen sulfide and ammonia, environment temperature 25~
27 DEG C, pH 6.5~7.5, biofilm tames 6~8d under conditions of humidity 38%~45%；
(4) biofilm domestication is passed through gas to be processed into bio-trickling filter after completing.
Preferably, in the step (1), Paracoccus denitrificans TS-1 is cultivated it is for the condition of Paracoccus denitrificans TS-1 bacterium solution
Cultivation temperature is 27~33 DEG C, medium pH is 6.0~7.0 and culture medium in C/N be 12~18.
Preferably, in the step (1), Paracoccus denitrificans TS-1 is cultivated it is for the condition of Paracoccus denitrificans TS-1 bacterium solution
Cultivation temperature is 30 DEG C, medium pH is 6.5 and culture medium in C/N ratio be 15.
Preferably, in the step (4), denitrogenation pair is incorporated as into bio-trickling filter while being passed through gas to be processed
The solution of nutrition needed for coccus TS-1 growth provides.
Preferably, application of the Paracoccus denitrificans in Non-aqueous processing.
The invention has the following advantages:
1, the nutrient type for the Paracoccus denitrificans TS-1 that invention is related to is amphitrophy type, realizes thallus under the conditions of heterotrophism
Quick breeding, realize high-efficiency desulfurization under autotrophic condition.Bacterial strain is expanded culture using organic carbon source Heterotrophic culture base
Obtain high density thallus, not only can under heterotrophism environment efficient degradation ammonia nitrogen, while can realize under autotrophic condition to sulfide
Oxidative degradation.
2, Paracoccus denitrificans TS-1 of the present invention has cracking ammonia nitrogen degradation rate, and 10h can under optimum condition
Degradation 170mg/L ammonia nitrogen, degradation rate is up to 17.0mg/ (L ﹒ h).
3, Paracoccus denitrificans TS-1 of the present invention has faster nitrate nitrogen degradation rate, which can be in aerobic item
Denitrification function is realized under part, by mineralized nitrogen for after nitrate nitrogen, nitrate nitrogen of degrading again generates nitrogen, is reached to the thorough of ammonium ion
Bottom removal.Under its optimum condition, degradable about 167mg/L, i.e. 1.67mg/ (Lh) in 10h.
4, the desulfuration efficiency of Paracoccus denitrificans TS-1 of the present invention is very fast, realizes in 6h under autotrophic condition
Elemental sulfur maximum conversion rate is up to 63.71% when 100% sulfide removal rate and 4.5h.
5, the present invention meets bio-safety regulation, and the Paracoccus denitrificans TS-1 from being coerced for a long time by high ammonia nitrogen and high sulfide
It screens and obtains in selection environment, will not cause damages to ambient enviroment and the ecological balance.
Detailed description of the invention
Fig. 1 is the cellular morphology of Paracoccus denitrificans TS-1 of the invention under a scanning electron microscope.
Fig. 2 is the growth curve that Paracoccus denitrificans TS-1 of the invention is cultivated in heterotrophism LB culture medium.
Fig. 3 is that the Paracoccus denitrificans TS-1 of the invention ammonia nitrogen degradation rate in degradation of ammonia nitrogen experimentation changes with time
Situation；
Fig. 4 is that the Paracoccus denitrificans TS-1 of the invention nitrate nitrogen degradation rate in degradation nitrate nitrogen experimentation changes with time
Situation；
Fig. 5 is that Paracoccus denitrificans TS-1 of the invention sulfide removal rate during desulfurization changes with time feelings
Condition；
Fig. 6 is that Paracoccus denitrificans TS-1 of the invention elemental sulfur conversion ratio during desulfurization changes with time feelings
Condition；
Fig. 7 is the form of the elemental sulfur of Paracoccus denitrificans TS-1 desulfurization formation of the invention under a scanning electron microscope；
The phylogenetic tree of Fig. 8 Paracoccus denitrificans TS-1.
Specific embodiment
The present invention will be described in detail with reference to embodiments.Embodiment is convenient to better understand the present invention, but is not pair
Limitation of the invention.
The culture medium being related in the following embodiments are as follows:
Activated sludge acclimatization culture medium (/L): KH2PO41.0g, K2HPO44.0g, MgCl20.5g, FeSO4 0.01g,
Glucose 5.0g, ammonia nitrogen and sulfide；Wherein the additive amount of ammonia nitrogen and sulfide see the table below:
Ammonia nitrogen and sulfide additive amount in one activated sludge acclimatization culture medium of table (/L)
| Ⅰ | Ⅱ | Ⅲ | Ⅳ | Ⅴ | |
| Na2S | 1.0 | 2.0 | 3.0 | 4.0 | 5.0 |
| (NH4)2SO4 | 0.8 | 1.6 | 2.4 | 3.2 | 4.0 |
Denitrification and desulfurization culture medium one (/L): glucose 5.0g, NaCl 2.0g, KH2PO41.0g, MgCl20.5g, FeSO4
0.01g, (NH4)2SO42.0g, Na2S 1.2g adjusts pH to 7.0 with the HCl solution of 1mol/L.
Denitrification and desulfurization culture medium two (/L): NaHS 5g.
LB culture medium (/L): tryptone 10.0g, yeast extract 5.0g, NaCl 10.0g, pH 7.0.The LB training
Base is supported for expanding culture.
Cyclic culture base (/L): glucose 6g, KH2PO41.0g, K2HPO41.0g, MgCl20.4g, NaHCO3 0.4g。
Embodiment 1: bacterial strain screening
It acquires Sichuan certain secondary sedimentation tank of sewage treatment work activated sludge 4L of double fluid to be added in reactor, and is equipped with 1L activated sludge
Domestication culture medium carries out aeration culture, is handled respectively with each concentration according to designed concentration gradient from low concentration to high concentration
5 days, 5 days, 5 days, 7 days, 8 days are the period, and domestication culture is completed after being tamed 30 days by low concentration to high concentration.Take a small amount of supernatant
It is enriched with 2 days in liquid denitrification and desulfurization culture medium one, gradient dilution is carried out after repeating 4~5 times, from 10-2、10-3、10-4、10-5、
10-6、10-7It draws 0.2mL under dilution to be coated in one plate of denitrification and desulfurization culture medium, 30 DEG C of constant temperature incubations, after separating for several times
Multiple bacterium colonies are obtained, the best single bacterium of picking growing state falls within the culture of denitrification and desulfurization culture medium one, is repeated 5 times achieved above pure
Bacterium, the bacterium are named as TS-1.The Physiology and biochemistry property of the bacterium is as follows:
The Physiology and biochemistry property of 2 Paracoccus denitrificans CGMCC NO.17605 of table
| Detection project | Testing result | Detection project | Testing result |
| Gram's staining | - | V.P | - |
| Thallus shape | It is spherical | Catalase | + |
| Oxidizing ferment | - | M.R | - |
| Starch Hydrolysis | + | Gelatin | + |
| Nitrate reduction test | + | Good/anaerobic | It is aerobic |
| Glucose | - | Maltose | - |
| L-arabinose | - | Lactose | - |
| Xylose | - | Grease | + |
Note: "+" indicates positive, and "-" indicates negative
Embodiment 2: bacterial strain identification
The bacterial strain isolated and purified is inoculated in denitrification and desulfurization culture medium one according to identical inoculum concentration, is placed in different temperatures
Shaking table in (5 DEG C, 10 DEG C, 24 DEG C, 27 DEG C, 30 DEG C, 33 DEG C, 36 DEG C, 40 DEG C, 45 DEG C, 50 DEG C) culture 12h, pass through measurement
OD600Determine that strain growth temperature is 10~50 DEG C, optimum growth temperature is 30 DEG C.Take different initial pH (3.0,4.0,5.0,
6.0, it 6.5,7.0,7.5,8.0,9.0,10.0,11.0) is cultivated at 30 DEG C for 24 hours, measures OD600Determine that bacterium growth pH is 4.0
~10.0, the most suitable growth pH are 6.5.Take different C/N (1,5,10,15,20,25,30) pH is 6.5, temperature is 30 DEG C
Under the conditions of measure OD600It is 5~25 that the tolerance range for determining the bacterial strain, which is C/N, and most suitable C/N is 15.
The bacterial strain isolated and purified is observed at scanning electron microscope (SEM), as a result as shown in Figure 1；The bacterium is quarter butt
Shape, long 0.5~0.9 μm, 0.4~0.7 μm wide, atrichia；Gram's staining identifies the bacterium for feminine gender.
Using bacterium full-length genome Rapid extraction kit, the full-length genome of pure bacterial strain is extracted, by selecting bacterium
16SrDNA universal primer 27F and 1492R carry out PCR amplification, then sequencing analysis.Sequencing result is through in ncbi database
BLAST is compared, and is identified that the bacterial strain is Paracoccus denitrificans, is named as Paracoccus denitrificans TS-1, as this hair
Bright preservation strain Paracoccus denitrificans Paracoccus denitrificans TS-1, is preserved in China on April 19th, 2019
Microbiological Culture Collection administration committee common micro-organisms center, address: Yard 1, BeiChen xi Road, Chaoyang District, Beijing City 3, China
Institute of microbiology, the academy of sciences, postcode: 100101, deposit number is CGMCC No.17605.
Growth curve research is carried out to Paracoccus denitrificans TS-1, selects LB culture medium to be cultivated, as a result such as 2 institute of attached drawing
Show: Paracoccus denitrificans TS-1 can in organic carbon source Heterotrophic culture base fast-growth, bacterium solution OD when cultivating 21h600Value just reaches
Bacterium solution OD after 1.0288,42h600Value maintains 1.23 or so, and the stage of stable development is up to 72h.The bacterial strain is numerous in Heterotrophic culture base
Grow growth quickly, and stationary phase is longer.Organic carbon source Heterotrophic culture base can be used in engineer application and be quickly obtained high density
Thallus not only has stronger advantage to ammonia nitrogen in efficiently quick removal waste water and sulfide, and being even more in biological deodorizing drop filter tower can
With rapid biofilm, quick start is that more there is provided preferable bacterium sources for engineering deodorization.
Embodiment 3: denitrification and desulfurization test is carried out using denitrification and desulfurization culture medium one
By one solution of 100mL denitrification and desulfurization culture medium, 115 DEG C of conditions first sterilize 30min in autoclave, then according to 5%
The Paracoccus denitrificans bacterium solution of inoculum concentration access culture for 24 hours, clogs bottleneck with aerobic, is placed in concussion (30 DEG C, 200r/ in shaking table
min)。
Control group, which is added, is arranged three repetitions with the sterile water of inoculum concentration equivalent, each experimental group, every 2h measure ammonia nitrogen,
Nitrate nitrogen, nitrous nitrogen concentration；Sulfide and simple substance sulphur concentration is measured by sampling every 0.5h, results are averaged.
Compared with blank control group, adds ammonia nitrogen removal efficiency in the culture medium of TS-1 bacterial strain and significantly improve, the ammonia in 12h
Nitrogen degradation rate reaches 95.35%, and the ammonia nitrogen concentration in blank control group culture medium does not have significant change, and removal rate is only
0.27%, illustrate the ability that the bacterial strain has stronger degradation of ammonia nitrogen.
The initial nitrate nitrogen of culture medium for adding TS-1 bacterial strain is about 70mg/L, and nitrate nitrogen concentration can be down to 4mg/L in 12 hours,
Degradation rate to nitrate nitrogen is 94.28%.Nitrate nitrogen concentration in blank control group does not decline a little raising instead in 12h, card
The bright bacterium denitrification significant effect.
The culture medium sulfide concentration of addition TS-1 bacterial strain is substantially reduced than control, and sulfide removal rate reaches when 6h
53%, 12h are that sulfide removal rate reaches 98%.Elemental sulfur in TS-1 culture medium its conversion ratio in 4.5h has reached
60%, maximum elemental sulfur production quantity can reach 486.58mg/L.In contrast in blank control group sulfide and sulphur simple substance conversion
Rate it can be seen that, the removal of sulfide in blank control group only simple physical removal, is not bioconversion removal, and
It is inoculated in the culture medium of the Paracoccus denitrificans, variation of valence has occurred in sulfide, it was demonstrated that it takes part in biological respinse.
Embodiment 4: denitrification and desulfurization test is carried out using denitrification and desulfurization culture medium two
By two solution of 100mL denitrification and desulfurization culture medium, 115 DEG C of conditions first sterilize 30min in autoclave, then according to 5%
The Paracoccus denitrificans bacterium solution of inoculum concentration access culture for 24 hours, clogs bottleneck with aerobic, is placed in concussion (30 DEG C, 200r/ in shaking table
Min), measuring initial ammonia nitrogen concentration is 170mg/L, and premature cure object concentration is 556mg/L.
Control group, which is added, is arranged three repetitions with the sterile water of inoculum concentration equivalent, each experimental group, every 2h measure ammonia nitrogen,
Nitrate nitrogen, nitrous nitrogen concentration；Sulfide and simple substance sulphur concentration is measured by sampling every 0.5h, results are averaged.
Its measurement result after 12h, as shown in attached drawing 3~6.
Compared with blank control group, adds ammonia nitrogen removal efficiency in the culture medium of TS-1 bacterial strain and significantly improve, the ammonia in 12h
Nitrogen degradation rate reaches 99.6%, and the ammonia nitrogen concentration in blank control group culture medium does not have significant change, and removal rate is only
0.27%, illustrate the ability that the bacterial strain has stronger degradation of ammonia nitrogen.
Attached drawing 4 show each factor situation of change of the Paracoccus denitrificans TS-1 in degradation nitrate nitrogen experimentation.Addition
The initial nitrate nitrogen of the culture medium of TS-1 bacterial strain is about 170mg/L, and nitrate nitrogen concentration can be down to 3mg/L in 10 hours, to the drop of nitrate nitrogen
Solution rate is up to 98.23%.Nitrate nitrogen concentration in blank control group does not decline a little raising instead in 10h, it was demonstrated that the bacterium is anti-
Nitrification effect is significant.
Attached drawing 5 show TS-1 to the degradation capability of sulfide in culture medium, it can be seen that its sulfide concentration compares illumination
Aobvious to reduce, the removal rate of sulfide may be up to 100% when sulfide removal rate reaches 58%, 12h when 6h.And it can also in attached drawing 6
It was found that its conversion ratio in 4.5h of the elemental sulfur in TS-1 culture medium has reached 66.7%, maximum elemental sulfur production quantity be can reach
523.46mg/L.In contrast to the sulfide and sulphur simple substance conversion ratio in blank control group it can be seen that, the vulcanization in blank control group
The removal of object is simple physical removal, is not bioconversion removal, and is inoculated in the culture medium of the Paracoccus denitrificans,
Variation of valence has occurred in sulfide, it was demonstrated that it takes part in biological respinse.
Shake flat experiment in integrated embodiment 2 and embodiment 3, it is possible to find Paracoccus denitrificans TS-1 is not only with higher different
Sample nitrification, the ability of aerobic denitrification, while also there is the stronger ability for removing sulphide removal, it is function there are three types of one plant while tools
The bacterial strain of energy.
Embodiment 5: bio-trickling filter Deodorization Experiment one
Paracoccus denitrificans TS-1 is cultivated in LB culture medium and obtains Paracoccus denitrificans TS-1 bacterium solution for 24 hours.Paracoccus denitrificans
During TS-1 is cultivated in LB culture medium, cultivation temperature is 27 DEG C, medium pH is 6.0 and culture medium in C/N be 12.
Cyclic culture base is added into the bio-trickling filter equipped with polyhedron empty ball filler, then by 2L culture for 24 hours de-
Nitrogen pair coccus TS-1 bacterium solution with wriggling pump circulation inject be equipped with filler bio-trickling filter, while with the air velocity of 4L/min,
Gas concentration 40mg/m3Hydrogen sulfide air inflow and gas concentration 40mg/m3Ammonia air inflow into bio-trickling filter simultaneously lead to
Enter air, hydrogen sulfide and ammonia, keep 25 DEG C of environment temperature, pH 6.5, humidity 38%, biofilm is tamed 6 days with this condition, is made
The bacterium can be stable be attached on filler.Then it is passed through ammonia into bio-trickling filter and hydrogen sulfide gas is removed ammonia
And the verification test of hydrogen sulfide.During being passed through ammonia and hydrogen sulfide gas is removed the confirmatory experiment of ammonia and hydrogen sulfide
Cyclic culture base, nutrition needed for Cyclic culture base provides growth for Paracoccus denitrificans TS-1 are added into bio-trickling filter.
In the present invention, biofilm domestication is to can adapt at one layer of the generation of multi-panel cavity filling surface and handle hydrogen sulfide and ammonia
The microbial film of gas.
Blank filler is added in control group, tests without strain biofilm.Circulation fluid is replaced to be tested with tap water.To reality
Test the ammonia and hydrogen sulfide that same concentrations are passed through while group biofilm is completed.Then the gas in two tower entrances and exit is recorded respectively
Bulk concentration, to observe removal and degradation capability of the Paracoccus denitrificans TS-1 to gas pollutant.Its result is as shown in the table.
Paracoccus denitrificans TS-1 deodorizing effect in 3 embodiment 5 of table
| The project indicator | Control group | Experimental group |
| Ammonia removal rate (%) | 27.22 | 100.0 |
| Hydrogen sulfide removal rate (%) | 17.23 | 96.34 |
Embodiment 6: bio-trickling filter Deodorization Experiment two
Paracoccus denitrificans TS-1 is cultivated in LB culture medium and obtains Paracoccus denitrificans TS-1 bacterium solution for 24 hours.Paracoccus denitrificans
During TS-1 is cultivated in LB culture medium, cultivation temperature is 30 DEG C, medium pH is 6.5 and culture medium in C/N be 15.
Cyclic culture base is added into the bio-trickling filter equipped with polyhedron empty ball filler, then by 2L culture for 24 hours de-
Nitrogen pair coccus TS-1 bacterium solution with wriggling pump circulation inject be equipped with filler bio-trickling filter, while with the air velocity of 5L/min,
Gas concentration 50mg/m3Hydrogen sulfide air inflow and gas concentration 50mg/m3Ammonia air inflow into bio-trickling filter simultaneously lead to
Enter air, hydrogen sulfide and ammonia, keep 26 DEG C of environment temperature, pH 7, humidity 41%, biofilm is tamed 6 days with this condition, makes this
Bacterium can be stable be attached on filler.Then be passed through ammonia into bio-trickling filter and hydrogen sulfide gas be removed ammonia and
The verification test of hydrogen sulfide.During being passed through ammonia and hydrogen sulfide gas is removed the confirmatory experiment of ammonia and hydrogen sulfide to
Cyclic culture base, nutrition needed for Cyclic culture base provides growth for Paracoccus denitrificans TS-1 are added in bio-trickling filter.
In the present invention, biofilm domestication is to can adapt at one layer of the generation of multi-panel cavity filling surface and handle hydrogen sulfide and ammonia
The microbial film of gas.
Blank filler is added in control group, tests without strain biofilm.Circulation fluid is replaced to be tested with tap water.To reality
Test the ammonia and hydrogen sulfide that same concentrations are passed through while group biofilm is completed.Then the gas in two tower entrances and exit is recorded respectively
Bulk concentration, to observe removal and degradation capability of the Paracoccus denitrificans TS-1 to gas pollutant.Its result is as shown in the table.
Paracoccus denitrificans TS-1 deodorizing effect in 4 embodiment 6 of table
| The project indicator | Control group | Experimental group |
| Ammonia removal rate (%) | 27.22 | 100.0 |
| Hydrogen sulfide removal rate (%) | 17.23 | 97.36 |
Embodiment 7: bio-trickling filter Deodorization Experiment three
Paracoccus denitrificans TS-1 is cultivated in LB culture medium and obtains Paracoccus denitrificans TS-1 bacterium solution for 24 hours.Paracoccus denitrificans
During TS-1 is cultivated in LB culture medium, cultivation temperature is 33 DEG C, medium pH is 7 and culture medium in C/N be 18.
Cyclic culture base is added into the bio-trickling filter equipped with polyhedron empty ball filler, then by 2L culture for 24 hours de-
Nitrogen pair coccus TS-1 bacterium solution with wriggling pump circulation inject be equipped with filler bio-trickling filter, while with the air velocity of 6L/min,
Gas concentration 60mg/m3Hydrogen sulfide air inflow and gas concentration 60mg/m3Ammonia air inflow into bio-trickling filter simultaneously lead to
Enter air, hydrogen sulfide and ammonia, keep 27 DEG C of environment temperature, pH 7.5, humidity 45%, biofilm is tamed 7 days with this condition, is made
The bacterium can be stable be attached on filler.Then it is passed through ammonia into bio-trickling filter and hydrogen sulfide gas is removed ammonia
And the verification test of hydrogen sulfide.During being passed through ammonia and hydrogen sulfide gas is removed the confirmatory experiment of ammonia and hydrogen sulfide
Cyclic culture base, nutrition needed for Cyclic culture base provides growth for Paracoccus denitrificans TS-1 are added into bio-trickling filter.
In the present invention, biofilm domestication is to can adapt at one layer of the generation of multi-panel cavity filling surface and handle hydrogen sulfide and ammonia
The microbial film of gas.
Blank filler is added in control group, tests without strain biofilm.Circulation fluid is replaced to be tested with tap water.To reality
Test the ammonia and hydrogen sulfide that same concentrations are passed through while group biofilm is completed.Then the gas in two tower entrances and exit is recorded respectively
Bulk concentration, to observe removal and degradation capability of the Paracoccus denitrificans TS-1 to gas pollutant.Its result is as shown in the table.
Paracoccus denitrificans TS-1 deodorizing effect in 5 embodiment 7 of table
| The project indicator | Control group | Experimental group |
| Ammonia removal rate (%) | 27.22 | 100.0 |
| Hydrogen sulfide removal rate (%) | 17.23 | 95.93 |
The deodorizing test of integrated embodiment 5~7, it is possible to find Paracoccus denitrificans TS-1 is reachable to the removal rate of ammonia in foul smell
100%, 97.36% is reached as high as to the removal rate of hydrogen sulfide gas, good deodorization effect can effectively remove hydrogen sulfide, ammonia etc.
Toxic and harmful gas, to be applied in deodorization and sewage treatment.
And without departing from the spirit or essential characteristics of the present invention, this can be realized in other specific forms
Invention.Therefore, in all respects, the present embodiments are to be considered as illustrative and not restrictive, the present invention
Range be indicated by the appended claims rather than the foregoing description, it is intended that the meaning that the equivalent requirements of the claims will be fallen in
It is included within the present invention with all changes in range.It should not treat any reference in the claims as involved by limitation
Claim.
In addition, it should be understood that although this specification is described in terms of embodiments, but not each embodiment is only wrapped
Containing an independent technical solution, this description of the specification is merely for the sake of clarity, and those skilled in the art should
It considers the specification as a whole, the technical solutions in the various embodiments may also be suitably combined, forms those skilled in the art
The other embodiments being understood that.
Sequence table
<110>Xi'an Polytechnic University
Chengdu Inst. of Biology, Chinese Academy of Sciences
<120>a kind of Paracoccus denitrificans bacterial strain screening and its application in deodorization
<160> 1
<170> SIPOSequenceListing 1.0
<210> 1
<211> 1327
<212> DNA
<213>Paracoccus denitrificans (Paracoccus denitrificans TS-1)
<400> 1
tcgctgcctc cattgctggt tagcgcacgg ccgtcgggta gacccaactc ccatggtgtg 60
acgggcggtg tgtacaaggc ccgggaacgt attcaccgcg gcatgctgtt ccgcgattac 120
tagcgattcc aacttcatgg ggtcgagttg cagaccccaa tccgaactga gatggctttt 180
ggggattaac ccactgtcac caccattgta gcacgtgtgt agcccaaccc gtaagggcca 240
tgaggacttg acgtcatcca caccttcctc cgacttatca tcggcagttc tcttagagtg 300
cccaaccaaa tgctggcaac taagagtgtg ggttgcgctc gttgccggac ttaaccgaac 360
atctcacgac acgagctgac gacagccatg cagcacctgt ccacaggtct cttacgagaa 420
aactccatct ctggagcggt cctgcgatgt caagggttgg taaggttctg cgcgttgctt 480
cgaattaaac cacatgctcc accgcttgtg cgggcccccg tcaattcctt tgagttttaa 540
tcttgcgacc gtactcccca ggcggaatgc ttaatccgtt aggtgtgtca ccgaacagca 600
tgctgcccga cgactggcat tcatcgttta cggcgtggac taccagggta tctaatcctg 660
tttgctcccc acgctttcgc acctcagcgt cagtatcgag ccagtgagcc gccttcgcca 720
ctggtgttcc tccgaatatc tacgaatttc acctctacac tcggaattcc actcacctct 780
ctcgaactcc agaccgatag ttttgaaggc agttccgagg ttgagccccg ggatttcacc 840
cccaactttc cggtccgcct acgtgcgctt tacgcccagt aattccgaac aacgctagcc 900
ccctccgtat taccgcggct gctggcacgg agttagccgg ggcttcttct gctggtaccg 960
tcattatctt cccagctgaa agagctttac aaccctaggg ccttcatcac tcacgcggca 1020
tggctagatc agggttgccc ccattgtcta agattcccca ctgctgcctc ccgtaggagt 1080
ctgggccgtg tctcagtccc agtgtggctg atcatcctct caaaccagct atggatcgtc 1140
ggcttggtag gccattaccc caccaactac ctaatccaac gcgggctaat cctttgccga 1200
taaatctttc ccccaaaggg cgtatacggt attactccca gtttcccggg gctattccgt 1260
agcaaagggc atattcccac gcgttactca cccgtccgcc gctaaccccg aagggtcgct 1320
cgactgc 1327
Claims (8)
1. a kind of Paracoccus denitrificans, it is characterised in that: strain name: Paracoccus denitrificans, Paracoccus denitrificans
TS-1, deposit number: CGMCC NO.17605.
2. a kind of Paracoccus denitrificans, it is characterised in that: its 16SrDNA sequence is as shown in SEQ ID No.1.
3. application of the Paracoccus denitrificans as claimed in claim 1 or 2 in deodorization.
4. application of the Paracoccus denitrificans in deodorization according to claim 3, which is characterized in that specific steps are as follows:
(1) Paracoccus denitrificans TS-1 is cultivated as Paracoccus denitrificans TS-1 bacterium solution, the work of Paracoccus denitrificans TS-1 in the bacterium solution
Bacterium amount is 108~109CFU/mL；
(2) solution for providing required nutrition for Paracoccus denitrificans TS-1 growth is added in the bio-trickling filter equipped with filler, so
Step (1) resulting Paracoccus denitrificans TS-1 bacterium solution is added afterwards；
(3) with the air velocity of 4~6L/min, 40~60mg/m of gas concentration3Hydrogen sulfide air inflow and gas concentration 40~
60mg/m3Ammonia air inflow into bio-trickling filter simultaneously be passed through air, hydrogen sulfide and ammonia, in environment temperature 25~27
DEG C, pH6.5~7.5, biofilm tames 6~8d under conditions of humidity 38%~45%；
(4) biofilm domestication is passed through gas to be processed into bio-trickling filter after completing.
5. application of the Paracoccus denitrificans in deodorization according to claim 4, it is characterised in that: in the step (1), will take off
Nitrogen pair coccus TS-1 culture be Paracoccus denitrificans TS-1 bacterium solution condition be cultivation temperature be 27~33 DEG C, medium pH 6.0
~7.0 and culture medium in C/N be 12~18.
6. application of the Paracoccus denitrificans in deodorization according to claim 5, it is characterised in that: in the step (1), will take off
Nitrogen pair coccus TS-1 culture be Paracoccus denitrificans TS-1 bacterium solution condition be cultivation temperature be 30 DEG C, medium pH be 6.5 and training
Supporting C/N ratio in base is 15.
7. application of the Paracoccus denitrificans in deodorization according to claim 4, it is characterised in that: in the step (4), logical
The solution of nutrition needed for Paracoccus denitrificans TS-1 growth provides is incorporated as while entering gas to be processed into bio-trickling filter.
8. application of the Paracoccus denitrificans as claimed in claim 1 or 2 in sewage treatment.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201910479735.XA CN110117567B (en) | 2019-06-04 | 2019-06-04 | Paracoccus denitrificans strain screening and application thereof in deodorization |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201910479735.XA CN110117567B (en) | 2019-06-04 | 2019-06-04 | Paracoccus denitrificans strain screening and application thereof in deodorization |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN110117567A true CN110117567A (en) | 2019-08-13 |
| CN110117567B CN110117567B (en) | 2023-03-03 |
Family
ID=67523704
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201910479735.XA Active CN110117567B (en) | 2019-06-04 | 2019-06-04 | Paracoccus denitrificans strain screening and application thereof in deodorization |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN110117567B (en) |
Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110452820A (en) * | 2019-08-16 | 2019-11-15 | 鹭滨环保科技（上海）股份有限公司 | A kind of screening of autotrophic denitrification bacterium and identification method |
| CN110452846A (en) * | 2019-08-16 | 2019-11-15 | 鹭滨环保科技（上海）股份有限公司 | A kind of Paracoccus denitrificans and its method with mineralising bed combination progress biological denitrificaion |
| CN114149944A (en) * | 2021-11-30 | 2022-03-08 | 南京农业大学 | Microorganism combination with tail end capable of efficiently adsorbing malodorous gas, enhanced colonization and application |
| CN114181856A (en) * | 2021-11-30 | 2022-03-15 | 南京农业大学 | Microbial composition for efficiently inhibiting odor production by biomass fermentation from source and application thereof |
| CN114958689A (en) * | 2022-06-29 | 2022-08-30 | 山东碧蓝生物科技有限公司 | Paracoccus, microbial inoculum and application thereof in livestock and poultry breeding deodorization field |
Citations (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102676430A (en) * | 2012-05-10 | 2012-09-19 | 大连理工大学 | Paracoccus denitrifican with denitrification and iron reduction functions and culturing method and application thereof |
| CN105400723A (en) * | 2015-12-18 | 2016-03-16 | 杭州楠溪生态环境科技有限公司 | Composite microbial preparation used for river treatment |
| CN106520617A (en) * | 2016-11-10 | 2017-03-22 | 中国科学院成都生物研究所 | Desulfurization and denitrification paracoccus denitrificans and application thereof |
| CN106834182A (en) * | 2017-02-27 | 2017-06-13 | 中国科学院成都生物研究所 | One plant of secondary meningitidis strains apt to change and its application |
| CN107090418A (en) * | 2017-05-17 | 2017-08-25 | 武汉科缘生物发展有限责任公司 | One strain denitrogen paracoccus and its application in livestock and poultry farm wastewater treatment |
| CN107760622A (en) * | 2017-11-03 | 2018-03-06 | 深圳克格瑞环保生物科技有限公司 | One strain denitrogen paracoccus and the method for high ammonia-nitrogen wastewater processing production single cell protein |
| CN107760626A (en) * | 2017-11-13 | 2018-03-06 | 中国科学院成都生物研究所 | One plant of Bangladesh's pair meningitidis strains and its application |
-
2019
- 2019-06-04 CN CN201910479735.XA patent/CN110117567B/en active Active
Patent Citations (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102676430A (en) * | 2012-05-10 | 2012-09-19 | 大连理工大学 | Paracoccus denitrifican with denitrification and iron reduction functions and culturing method and application thereof |
| CN105400723A (en) * | 2015-12-18 | 2016-03-16 | 杭州楠溪生态环境科技有限公司 | Composite microbial preparation used for river treatment |
| CN106520617A (en) * | 2016-11-10 | 2017-03-22 | 中国科学院成都生物研究所 | Desulfurization and denitrification paracoccus denitrificans and application thereof |
| CN106834182A (en) * | 2017-02-27 | 2017-06-13 | 中国科学院成都生物研究所 | One plant of secondary meningitidis strains apt to change and its application |
| CN107090418A (en) * | 2017-05-17 | 2017-08-25 | 武汉科缘生物发展有限责任公司 | One strain denitrogen paracoccus and its application in livestock and poultry farm wastewater treatment |
| CN107760622A (en) * | 2017-11-03 | 2018-03-06 | 深圳克格瑞环保生物科技有限公司 | One strain denitrogen paracoccus and the method for high ammonia-nitrogen wastewater processing production single cell protein |
| CN107760626A (en) * | 2017-11-13 | 2018-03-06 | 中国科学院成都生物研究所 | One plant of Bangladesh's pair meningitidis strains and its application |
Non-Patent Citations (11)
| Title |
|---|
| FANG Y等: "Sulfide oxidation and nitrate reduction for potential mitigation of H2S in landfills", 《BIODEGRADATION》 * |
| MA H等: "The Odor Release Regularity of Livestock and Poultry Manure and the Screening of Deodorizing Strains", 《MICROORGANISMS》 * |
| VILLAHERMOSA, D等: "Reduction of Net Sulfide Production Rate by Nitrate in Wastewater Bioreactors. Kinetics and Changes in the Microbial Community", 《WATER AIR AND SOIL POLLUTION》 * |
| 佟欣宇等: "一株高效异养脱硫菌的筛选及性能研究", 《化学工程师》 * |
| 佟海等: "异养脱氮除硫菌株的筛选与条件优化", 《太原理工大学学报》 * |
| 孙将等: "高效除氨氮异养硝化细菌的分离鉴定及其脱氮条件优化", 《农业工程学报》 * |
| 李海红等: "高效异养硝化-好氧反硝化菌株TS-1筛选及降解特性", 《应用与环境生物学报》 * |
| 梁书诚: "高效好氧反硝化细菌的筛选及脱氮特性研究", 《中国优秀硕士学位论文全文数据库工程科技Ⅰ辑》 * |
| 樊杰等: "一株硝化脱氮除臭菌的筛选鉴定及其多途径氮代谢功能的研究", 《环境科学学报》 * |
| 田凤蓉等: "高效脱氨除臭异养硝化菌的筛选鉴定及脱氨性能研究", 《环境工程》 * |
| 袁梦冬等: "1株异养硝化-好氧反硝化菌的分离鉴定及脱氮活性", 《北华大学学报(自然科学版)》 * |
Cited By (9)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110452820A (en) * | 2019-08-16 | 2019-11-15 | 鹭滨环保科技（上海）股份有限公司 | A kind of screening of autotrophic denitrification bacterium and identification method |
| CN110452846A (en) * | 2019-08-16 | 2019-11-15 | 鹭滨环保科技（上海）股份有限公司 | A kind of Paracoccus denitrificans and its method with mineralising bed combination progress biological denitrificaion |
| CN110452846B (en) * | 2019-08-16 | 2022-07-12 | 鹭滨环保科技（上海）股份有限公司 | Paracoccus denitrificans and biological denitrification method by combining paracoccus denitrificans with mineralization bed |
| CN114149944A (en) * | 2021-11-30 | 2022-03-08 | 南京农业大学 | Microorganism combination with tail end capable of efficiently adsorbing malodorous gas, enhanced colonization and application |
| CN114181856A (en) * | 2021-11-30 | 2022-03-15 | 南京农业大学 | Microbial composition for efficiently inhibiting odor production by biomass fermentation from source and application thereof |
| CN114181856B (en) * | 2021-11-30 | 2023-12-15 | 南京农业大学 | Microbial combination capable of effectively inhibiting odor production of biomass fermentation from source and application thereof |
| CN114149944B (en) * | 2021-11-30 | 2024-04-09 | 南京农业大学 | Microorganism combination capable of efficiently adsorbing malodorous gas at tail end, enhanced colonization and application |
| CN114958689A (en) * | 2022-06-29 | 2022-08-30 | 山东碧蓝生物科技有限公司 | Paracoccus, microbial inoculum and application thereof in livestock and poultry breeding deodorization field |
| CN114958689B (en) * | 2022-06-29 | 2023-03-10 | 山东碧蓝生物科技有限公司 | Paracoccus, microbial inoculum and application thereof in livestock and poultry breeding deodorization field |
Also Published As
| Publication number | Publication date |
|---|---|
| CN110117567B (en) | 2023-03-03 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN110117567A (en) | A kind of Paracoccus denitrificans bacterial strain screening and its application in deodorization | |
| CN104312938B (en) | Pseudomonas putida strain and fungicide and application of pseudomonas putida strain | |
| CN107586745B (en) | Livestock manure compost deodorization nitrogen-retention strain, microbial inoculum and preparation method and application thereof | |
| CN113151034B (en) | Bacillus subtilis strain for deodorizing livestock and poultry breeding environment | |
| CN113969246B (en) | Nitrogen-preserving strain, composite microbial inoculum, and preparation method and application of composite microbial inoculum | |
| CN111676150B (en) | Efficient deodorant bacterium and application thereof | |
| CN108977398B (en) | Bacillus megaterium and application thereof | |
| JP2004248618A (en) | Bacterial group symbiotically living with fungus used for treating organic material and its application | |
| CN107345213B (en) | Lysine bacillus for degrading dairy cow excrement acid odor and application thereof | |
| CN104673724A (en) | Composite photosynthetic bacteria preparation applied to sewage treatment and preparation method of composite photosynthetic bacteria preparation | |
| CN108018250A (en) | One plant of Acidithiobacillus ferrooxidans strain GF and its application in environmental improvement | |
| CN114890555B (en) | Solid microbial preparation for treating rural black and odorous water body and preparation method and application thereof | |
| CN113151120B (en) | Sulfur oxidizing bacteria and uses thereof | |
| CN109486725B (en) | Bacterial strain capable of degrading benzene series and petroleum hydrocarbon and application thereof | |
| CN105441345A (en) | Candida tropicalis and application thereof to biological desulphurization | |
| CN108102978B (en) | Degradation strain JN8 for petroleum hydrocarbons in oily sludge and application thereof | |
| CN113897313A (en) | Preparation method and application of composite microbial deodorant bacterial agent | |
| CN109652328B (en) | Composite microorganism live bacteria preparation and application thereof in high-concentration pig-raising wastewater | |
| CN113337441B (en) | High-temperature-resistant sulfur oxidizing strain LYH-2 and application thereof | |
| CN108660087A (en) | A kind of complex micro organism fungicide and its preparation method and application for reducing the release of Chicken Manure Compost ammonia | |
| CN114874938A (en) | Bacillus cereus S5 for degrading hydrogen sulfide gas and application thereof | |
| CN110184213B (en) | Water-producing Alcaligenes DF-27 strain and application thereof | |
| CN108048376B (en) | Degradation strain JN3 for petroleum hydrocarbons in oily sludge and application thereof | |
| CN110894496A (en) | Immobilized microbial agent and preparation method and application thereof | |
| CN106520617B (en) | One plant of desulfurization removing nitric pair coccus and its application |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| GR01 | Patent grant | ||
| GR01 | Patent grant |