CN108018250A - One plant of Acidithiobacillus ferrooxidans strain GF and its application in environmental improvement - Google Patents
One plant of Acidithiobacillus ferrooxidans strain GF and its application in environmental improvement Download PDFInfo
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Abstract
The invention discloses one plant of Acidithiobacillus ferrooxidans strain GF and its application in environmental improvement, the Acidithiobacillus ferrooxidans strain GF is Acidithiobacillus ferrooxidans strain GF（Acidithiobacillus ferrooxidans ）DY 1, deposit number are：CCTCC NO：M2017806, by good biological oxidation, the acid effect of biology and stronger metal tolerance, metal adsorption ability etc., Acidithiobacillus ferrooxidans strain GF DY 1 has significant effect in the de- black deodorization of black and odorous water, the restoration of the ecosystem of bed mud etc., and original water system will not be damaged.The pioneer that can be administered as heavy polluted water, good water quality and ecological microenvironment basis are established subsequently further to improve water quality using administering methods such as other microorganisms, water plants, recovering water ecology balance.
Description
Technical field
The invention belongs to the technical field of microbial treatment pollution environment, and in particular to one plant of Acidithiobacillus ferrooxidans strain GF
And its application in environmental improvement.
Technical background
In recent years, water environment degradation is particularly problematic, and the universal eutrophication in lake, cyanobacteria break out again and again and river becomes silted up
A series of problems, such as product, function decay, black and odorous water, heavy metal pollution, not only compromise the living environment of the common people, also serious shadow
Ring city image.With the improvement of living standards, the expectation that people improve environmental improvement is higher and higher, environmental protection work is just
The new normality that Opportunity coexists with challenge is faced, wherein water environment treatment has been also raised to a unprecedented height.
Microorganism remediation mainly the pollutant accumulated in water body is absorbed using microorganism vigorous vital movement,
Degraded and conversion, polishing metabolism missing link, the Food web of dredge blockage, adjusts water ecology, fundamentally solves water environment
Pollution problem.In recent years, the advantage of microorganism remediation is increasingly prominent, becomes the hot spot of water environment treatment reparation.But for weight
The water body of pollution is spent, especially black and odorous water, beneficial microorganism activity is suppressed, and makes microorganism in the water of high pollution concentration
It is limited in the improvement of body, especially black and odorous water.
The present invention isolates one plant of Acidithiobacillus ferrooxidans strain GF DY-1 from the slag in Daye mine, existing thermophilic with other
Acid oxidase ferrous iron Thiobacillus bacterial strain compared to have the advantages that reproduction speed is fast, durability against pollution is strong, it is adaptable, have a wide range of application,
It can be used for the improvement of the water environments such as de- black deodorization, the restoration of the ecosystem of bed mud of black and odorous water, it may also be used for biological deodorizing, biology
Soak ore deposit, sludge drip leaching, metal recovery, the field such as sewage disposal, with it is quick, cost is low, safety and environmental protection, non-secondary pollution etc.
Advantage, has promotional value.The present invention will administer for aquatic ecological restoration provides a kind of new idea and method.
The content of the invention
It is an object of the invention to administer the problem of difficult for the black and odorous water in environment and bed mud, there is provided one plant of acidophilus
Thiobacillus ferrooxidans, the bacterium are preserved in China typical culture collection center, Classification And Nomenclature on December 19th, 2017：It is thermophilic
Acid oxidase ferrous iron Thiobacillus Acidithiobacillus ferrooxidans DY-1, deposit number：CCTCC NO：
M2017806, hereinafter referred to as Acidithiobacillus ferrooxidans strain GF DY-1, address：Wuhan, China Wuhan University.
It is another object of the present invention to provide a kind of Acidithiobacillus ferrooxidans strain GF in environmental improvement, especially black smelly
Application in water body treating and bed mud restoration of the ecosystem.
For achieving the above object, technical scheme is as follows：
The acquisition of Acidithiobacillus ferrooxidans strain GF DY-1：
Applicant, through repeatedly enrichment, purifies and separates, is finally obtained one plant of acidophilus from the slag in Daye, hubei Province mine
Thiobacillus ferrooxidans.The bacterium is preserved in China typical culture collection center, deposit number on December 19th, 2017：
CCTCC NO：M2017806, Classification And Nomenclature are：Acidithiobacillus ferrooxidans strain GF Acidithiobacillus ferrooxidans
DY-1, hereinafter referred to as Acidithiobacillus ferrooxidans strain GF DY-1, address：Wuhan, China Wuhan University.
The Acidithiobacillus ferrooxidans strain GF is Gram-negative bacteria, and suitable growth temperature is 28~35 DEG C, most suitable life
Long pH is 2.0~3.5, is cultivated in 9K culture mediums.Colonial morphology is small and convex circular single bacterium colony, 1~1.5mm of diameter, bacterium
Body is in glassy yellow, and there is the small protrusion of black at center, and colony edge is regular.Micro- Microscopic observation usually with it is single, two or several
In chainlike distribution, single bacterium is in being brevibacterium, and thalline is about 1~1.5 μm, about 0.5 μm of diameter, both ends blunt circle, amphitrichous, energy
Active movement.
The physiological and biochemical property of the Acidithiobacillus ferrooxidans strain GF DY-1 is：The glycoxidative production acid of grape is positive, grape
Sugared fermentation and acid aerogenesis is negative, Oxidation of Fe2+、FeS2It is positive, aoxidizes S, S2O3 2-It is positive, acidophilia is stringent aerobic.
The composition of 9K fluid nutrient mediums includes：A liquid：(NH4)2SO4 3.0g/L、KCl 0.1g/L、K2HPO4 0.5g/L、
MgSO4·7H2O 0.5g/L、Ca(NO3)20.01g/L, two citric acid monohydrate trisodiums 8.823g/L, distilled water；B liquid：FeSO4·
7H2O 44.7g/L, distilled water, adds appropriate 1:1H2SO4Solution, adjusts pH, suppresses Fe2+Oxidation.121 DEG C of high-temperature sterilizations of A liquid
105 DEG C of 30min, B liquid sterilizing 15min, mix after cooling, and with 1 to sterilize:1H2SO4Solution adjusts pH to 2.0.
The composition of 9K solid mediums includes：1.5% agar is added on the basis of above-mentioned 9K fluid nutrient mediums.
The Acidithiobacillus ferrooxidans strain GF DY-1 is in 9K fluid nutrient mediums with the extension of incubation time, nutrient solution
Color undergo the change procedure of " light green-pale yellow-orange-orange red-dark red-reddish brown " successively, fermentation period is short, easy production, hair
Ferment is at concentrations up to 108~1010cfu/mL。
The Acidithiobacillus ferrooxidans strain GF DY-1, with Fe2+For energy substance, by by Fe in growth course2+Oxygen
It is melted into Fe3+To obtain the energy needed for growth, and with CO2For carbon source, the growth that the inorganic nutrients such as absorbed nitrogen, phosphorus carry out itself is numerous
Grow.Shown in its oxidation mechanism such as formula (1), (2)：
Under above-mentioned effect, Acidithiobacillus ferrooxidans strain GF DY-1 possesses good biological oxidation and biological acidification
Effect.In field of environmental improvement, Acidithiobacillus ferrooxidans strain GF DY-1 utilizes Fe excessive in water environment2+As the energy, it is used for
The growth and breeding of itself, while utilize the Fe with strong oxidizing property discharged3+Water body will be suspended in or be deposited in bed mud
Simple substance S or S2-、S2O3 2-、S4O6 2-Decomposed etc. soluble or insoluble sulfide, be oxidized to SO4 2-, reduce the cause in environment
Black material, promote black and odorous water and bed mud take off it is black.On the other hand, the biological oxygen built using Acidithiobacillus ferrooxidans strain GF DY-1
Change, acidifying microenvironment, realize the oxidation, degraded and conversion of organic matter, so as to improve water transparency, reduce the pollution of water environment
Load.With odor causing substances such as oxygenolysis hydrogen sulfide, ammonia, mercaptan, the odor concentration of environment can be reduced.
Heteroglycan containing complexity in the cell wall matrix of Acidithiobacillus ferrooxidans strain GF DY-1 of the present invention, comprising
Amino, amide groups and carboxyl etc., the more other acidophilic microorganisms of its structure are more complete, and DY-1 floras can also secrete Fe oxidations
Enzyme to form the biological iron oxide particle of cell combination to be catalyzed, and changes antimicrobial surface activity, strengthens the affinity to metal
And adsorption capacity.Therefore Acidithiobacillus ferrooxidans strain GF DY-1 of the present invention has stronger metal tolerance and more powerful
Metal adsorption ability, removal, recycling to heavy metal in environment, utilize etc. and to have positive realistic meaning.
Acidithiobacillus ferrooxidans strain GF DY-1 of the present invention can be used to prepare the cleanser of black and odorous water, which can
Polluted-water and bed mud middle or low price sulfide, the oxygenolysis of part organic matter are completed in short time, promotes water body quickly to take off black
Deodorization, enhances the transparency.
Acidithiobacillus ferrooxidans strain GF DY-1 of the present invention can be used to prepare the modifying agent of bed mud in black and odorous water, should
Black smelly bed mud can be deodorized by bacterium, promoted the passivation of bed mud metal pollutant to eliminate, realized ecological recovery.
Acidithiobacillus ferrooxidans strain GF DY-1 of the present invention can be used to prepare sulfide scavenger in black and odorous water, go
Except rate is up to more than 95%.
Acidithiobacillus ferrooxidans strain GF DY-1 of the present invention can be used to prepare oxygenolysis hydrogen sulfide, ammonia, mercaptan
The distintegrant of material with foul odour, wherein hydrogen sulfide removal rate are up to more than 99%, and deodorization is efficiently, thoroughly, do not rebound.
Acidithiobacillus ferrooxidans strain GF DY-1 of the present invention can be used for the removal of the heavy metal in black and odorous water, can use
In preparing heavy metals removal agent；Preferably, respectively reach 95~99.5% to the removal rate of Zn, Cu, Cd, Cr, 92~98%,
90~99%, 90~99%, it is stronger to the tolerance of heavy metal, the tolerable concentration of Zn, Cu, Cd, Cr is followed successively by 1800mg/L,
1200mg/L, 800mg/L and 500mg/L.
Compared with prior art, the present invention has the following advantages：
(1) the present invention provides a kind of Acidithiobacillus ferrooxidans strain GF DY-1 screened from environment, compared to other
It was found that Acidithiobacillus ferrooxidans strain GF, bacterial strain DY-1 be one plant of reproduction speed it is fast, easily production, it is adaptable, safe and efficient, should
With extensive Acidithiobacillus ferrooxidans strain GF.
(2) Acidithiobacillus ferrooxidans strain GF is applied to the improvement of surface water environment by the present invention first, is water environment treatment
Provide new approaches.By good biological oxidation, the acid effect of biology and stronger metal tolerance, metal adsorption
Ability etc., Acidithiobacillus ferrooxidans strain GF DY-1 have significantly in the de- black deodorization of black and odorous water, the restoration of the ecosystem of bed mud etc.
Effect, and original water system will not be damaged.The pioneer that can be administered as heavy polluted water, subsequently to use other micro- lifes
The administering methods such as thing, water plant further improve water quality, recovery water ecology balance establishes good water quality and ecology
Microenvironment basis.
(3) Acidithiobacillus ferrooxidans strain GF DY-1 provided by the invention can create good biological oxidation environment and biology
Acidified environment, hence it is evident that eliminate stench, build the pleasant ecological environment of health.
(4) Acidithiobacillus ferrooxidans strain GF DY-1 provided by the invention is returned in deodorization, sludge bioleaching, leaching ore deposit, metal
Receipts, the microorganism processing of mineral material and acid waste water processing etc. also have significant effect, and acidophilus oxidation is sub-
Iron Thiobacillus DY-1 is easy to operate in actual application, significant effect, adaptable, with strong points, environmentally protective, nothing two
It is secondary to pollute, is of low cost, can be flexibly supporting with other Treatment process, it is suitable for large-scale promotion.
Brief description of the drawings
Fig. 1 is Acidithiobacillus ferrooxidans strain GF DY-1 colonial morphology schematic diagrames.
Fig. 2 is the form schematic diagram under microscope after Acidithiobacillus ferrooxidans strain GF DY-1 Gram's staining.
Embodiment
With reference to specific embodiment, the present invention is further explained.Experiment material used in following embodiments, such as without spy
Different explanation, is to be commercially available from routine biochemistry reagent shop, specific experiment condition and method is not specified in the following example, is led to
Often obtained according to cellar culture and separation method.
Embodiment 1
The acquisition of Acidithiobacillus ferrooxidans strain GF DY-1 bacterial strains.
Sample is gathered from the slag in Daye, hubei Province mine, and through repeatedly enrichment, purifying, obtains single bacterium colony bacterial strain and reflects
It is fixed.
The Acidithiobacillus ferrooxidans strain GF DY-1 bacterial strains isolate and purify process：Take 5g slag samples be dissolved in 50mL without
Bacterium water, 28 DEG C, 180r/min shaken cultivation 6h, then stands 2h.Absorption 10mL upper stratas water sample containing strain, which is added to, fills 100mL
In sterilized 9K fluid nutrient mediums, 28 DEG C, 180r/min shaking table cultures, until culture medium becomes rufous by light green color, then
5 enrichment cultures are carried out, after the time occurred to rufous becomes 1d by original 5d, using solid medium spread plate method
Separation, purify strain to obtain pure culture single strain.
The inoculation of the Acidithiobacillus ferrooxidans strain GF DY-1 is into 9K fluid nutrient mediums, with incubation time
Extend, the color of nutrient solution undergoes the change procedure of " light green-pale yellow-orange-orange red-dark red-reddish brown " successively, this is because carefully
Bacterium is in vital movement constantly by Fe2+It is oxidized to Fe3+, to obtain the energy needed for life.Continue to cultivate, can gradually have a large amount of
Yellow mercury oxide generates, and precipitation is identified as the mixture of ammonium jarosite and autunezite, is the Fe of solution middle and high concentration3+In acidity
Under the conditions of hydrolyze the product of generation.
The Acidithiobacillus ferrooxidans strain GF DY-1, its Classification And Nomenclature are：Acidithiobacillus ferrooxidans strain GF
Acidithiobacillus ferroosidans DY-1, have been preserved in Chinese Typical Representative culture guarantor on December 19th, 2017
Tibetan center, deposit number：CCTCC NO：M2017806, address：Wuhan, China Wuhan University.
Embodiment 2
The strain property of Acidithiobacillus ferrooxidans strain GF DY-1：
1st, the microscopy record after Morphological Identification, including the observation of colonial morphology, description and Gram's staining.Feature is as follows：
28 DEG C of culture 7d of solid medium, form small and convex circular single bacterium colony, thalline is in glassy yellow, and there are the small protrusion of black, bacterium in center
Fall Edge divider (Fig. 1)；Gram's staining, microscopy result are Gram-negative.Micro- Microscopic observation usually with it is single, two or
It is in chainlike distribution that person is several, and single bacterium is in being brevibacterium, about 1~1.5 μm of thalline length, about 0.5 μm of diameter, both ends blunt circle,
Amphitrichous, can vivaciously move (Fig. 2).
2nd, 16S rDNA Molecular Identifications, i.e., using prokaryotes 16S rDNA universal primer 27F and 1492R (27F sequences：-
AGAGTTTGATCCTGGCTCAG-, 1492R sequence：- GGTTACCTTGTTACGACTT-) carry out PCR sequence amplifications.Expand in PCR
After increasing, by agarose gel electrophoresis, gel extraction purpose fragment, is measured its sequence.Measurement result and the world
Information on NCBI GenBank (www.ncbi.nih.gov) RiboaptDB compares, the bacterial strain and acidophilus ferrous oxide sulphur
Bacillus homology is up to 100%.
3rd, Physiology and biochemistry is identified.The physiological and biochemical property of the Acidithiobacillus ferrooxidans strain GF DY-1 is：Grape glycosyloxy
It is positive to change production acid, the sour aerogenesis of glucose fermentation production is negative, Oxidation of Fe2+、FeS2It is positive, aoxidizes S, S2O3 2-It is positive, acidophilus
Property, it is stringent aerobic.Suitable growth temperature is 28~30 DEG C, and the most suitable growth pH is 2.0~3.5, and growth logarithmic phase is 18~30h.
The physiological and biochemical property of 1 Acidithiobacillus ferrooxidans strain GF DY-1 of table
Embodiment 3
Applications of the Acidithiobacillus ferrooxidans strain GF DY-1 in black and odorous water takes off black deodorization.
Black smelly water sample picks up from Wuhan black-odor riverway used in experiment.Experimental provision is internal diameter 10cm, the cylinder of high 35cm
Container, loads the black smelly water samples of 1L.Experiment is divided into experimental group and control group.Experimental group accesses the DY-1 bacterium of 10% (volume ratio) activation
Suspension, control group add the sterile purified water of equivalent.3 repetitions of every group of processing, are placed at 28 DEG C, are aerated logical oxygen.Take respectively
The water sample of 12h, 24h, 48h, 72h period carry out Fe2+Oxygenation efficiency and S2-, organic matter, ammonia nitrogen removal rate analysis, as a result
It is shown in Table 2.
Fe2+Oxygenation efficiency can reflect the activity of bacterial strain DY-1 thalline.After throwing bacterium 24h, Fe2+Oxygenation efficiency maintain essentially in
More than 95%, illustrate that bacterial strain DY-1 can keep higher activity in the black smelly water of high concentration.In addition, bacterial strain DY-1 is to black and odorous water
In sulfide, organic matter have an obvious removal effect, the removal rate of sulfide is 99.7%, and the removal rate of organic matter is about
61%th, the removal rate of ammonia nitrogen is 59%, and the removal efficiency than control group aeration oxygen replenishing is high.The sense organ of black and odorous water also has bright
Aobvious improvement, reacts to 48h, water sample and gradually by black smelly transformation yellowly, almost can't smell stink, control group smell slightly has
Turn, but water sample is in still black.
Treatment effects of the 2 Acidithiobacillus ferrooxidans strain GF DY-1 of table to black and odorous water
Embodiment 4
Tolerances of the Acidithiobacillus ferrooxidans strain GF DY-1 to heavy metal：
Experimental provision is internal diameter 10cm, the hydrostatic column of high 35cm.Four kinds of (Zn are separately added into reaction unit2+、
Cu2+、Cd2+、Cr6+) various concentrations gradient metal ion and 9K culture medium mixed solution 1L, the DY-1 that access 10% has activated
Bacteria suspension.3 repetitions of every group of processing, are placed at 28 DEG C, are aerated logical oxygen 72h, every the upgrowth situation of 6h detection thalline, that is, survey
Determine Fe2+Oxygenation efficiency.
Zn2+Concentration gradient：500mg/L、1000mg/L、1500mg/L、1800mg/L、2000mg/L；Cu2+Concentration gradient：
300mg/L、500mg/L、800mg/L、1000mg/L、1200mg/L、1500mg/L；Cd2+Concentration gradient：100mg/L、200mg/
L、300mg/L、500mg/L、800m/L、1000mg/L；Cr6+Concentration gradient：100mg/L、300mg/L、500mg/L、800mg/
L、1000mg/L。
After heavy metal concentration described above is access bacterium solution, the final concentration of each heavy metal in solution.
According to Fe2+The analysis result of oxygenation efficiency, bacterial strain DY-1 are followed successively by 1800mg/ to the tolerable concentration of Zn, Cu, Cd, Cr
L, 1200mg/L, 800mg/L and 500mg/L.Below above-mentioned each concentration of heavy metal ion, Acidithiobacillus ferrooxidans strain GF DY-1
To Fe2+Oxygenation efficiency maintain more than 90%, thalline keeps greater activity in reactor；Tolerance more than above-mentioned each heavy metal is dense
Degree, Fe2+Oxygenation efficiency reduce rapidly, thalline activity be substantially suppressed.It is known under normal circumstances, Zn, Cu, Cd and Cr element
5~13mg/L, 0.5~1.0mg/L, 1~5mg/L, 2~5mg/L are followed successively by the maximum permissible concentration of microorganism toxic action
(different maximum permissible concentrations are with different microorganisms and different), therefore it is used for the microbial ratio of water environment treatment, acidophilus oxygen with other
Changing ferrous iron Thiobacillus DY-1 bacterial strains has remarkable heavy metal tolerance.
Embodiment 5
Acidithiobacillus ferrooxidans strain GF DY-1 is containing Zn2+Black and odorous water in activity and the removal effect to pollutant：
Black smelly water sample picks up from Wuhan black-odor riverway used in experiment.Experimental provision is internal diameter 10cm, the cylinder of high 35cm
Container.1L is passed through into reaction unit respectively and contains Zn2+The black smelly water sample of final concentration of 50mg/L, 500mg/L.Every group of processing 3
Repeat, be placed at 28 DEG C, be aerated logical oxygen.The water sample of 12h, 24h, 48h, 72h period is taken to carry out Fe respectively2+Oxygenation efficiency and
Zn2+、S2-, organic matter and ammonia nitrogen removal rate analysis.
Fe2+Oxygenation efficiency can preferably reflect the activity of bacterial strain DY-1.In 12h, DY-1 bacterium are in the laundering period, in 24h
During~48h, DY-1 bacterium grow into dominant bacteria, Fe2+Oxidation efficiency peaks the phase, and the removal efficiency of pollutant reaches higher
It is horizontal；Reaction is to 72h, and the concentration of pollutant reduces, therefore treatment effeciency also decreases.In low Zn2+Under conditions of (50mg/L),
Fe2+Oxygenation efficiency maintain essentially in more than 90%, illustrate DY-1 bacterium well-grown, Zn under 50mg/L concentration2+Removal rate reach
97%, S2-Removal rate up to 98%, the removal rate of organic matter is 60%, and the removal rate of ammonia nitrogen is 58%.High Zn2+Under the conditions of, by
High concentration Zn2+The influence of (500mg/L), the activity of bacterial strain DY-1 slightly reduce, and the removal efficiency and low concentration of pollutant are basic
Unanimously, influence less.
3 bacterial strain DY-1 of table contains Zn in difference2+Activity in the black and odorous water of amount and the removal effect to pollutant
Embodiment 6
Acidithiobacillus ferrooxidans strain GF DY-1 is containing Cu2+Black and odorous water in activity and the removal effect to pollutant：
Black smelly water sample picks up from Wuhan black-odor riverway used in experiment.Experimental provision is internal diameter 10cm, the cylinder of high 35cm
Container.Cu is passed through into reaction unit respectively2+The black smelly water sample of final concentration of 10mg/L and 100mg/L.3 weights of every group of processing
It is multiple, it is placed at 28 DEG C, is aerated logical oxygen.The water sample of 12h, 24h, 48h, 72h period is taken to carry out Fe respectively2+Oxygenation efficiency and
Cu2+、S2-, organic matter and ammonia nitrogen removal rate analysis.
Under conditions of low Cu (10mg/L), Fe2+Oxygenation efficiency maintain essentially in more than 85%, illustrate that DY-1 bacterium exist
Well-grown under 10mg/L concentration, Cu2+Removal rate up to 95%, S2-Removal rate up to 98%, the removal rate of organic matter is
63%th, ammonia nitrogen removal frank 55%.Under the conditions of high Cu (100mg/L), by high concentration Cu2+Influence, bacterial strain DY-1 activity slightly
There is reduction, but the removal efficiency of pollutant maintains essentially in higher level.
4 bacterial strain DY-1 of table contains Cu in difference2+Activity in the black and odorous water of amount and the removal effect to pollutant
Embodiment 7
Acidithiobacillus ferrooxidans strain GF DY-1 is containing Cd2+Black and odorous water in activity and the removal effect to pollutant：
Black smelly water sample picks up from Wuhan black-odor riverway used in experiment.Experimental provision is internal diameter 10cm, the cylinder of high 35cm
Container.Cd is passed through into reaction unit respectively2+The black smelly water sample of final concentration of 10mg/L and 100mg/L.3 weights of every group of processing
It is multiple, it is placed at 28 DEG C, is aerated logical oxygen.The water sample of 12h, 24h, 48h, 72h period is taken to carry out Fe respectively2+Oxygenation efficiency and
Cd2+、S2-、The removal rate of organic matter and ammonia nitrogen is analyzed.
In low Cd2+Under conditions of (10mg/L), Fe2+Oxygenation efficiency maintain essentially in more than 85%, illustrate that DY-1 bacterium exist
Well-grown under 10mg/L concentration.Under this concentration, Cd2+Removal rate up to 96%, S2-Removal rate up to 95%, organic matter is gone
Except rate is 56%, ammonia nitrogen removal frank 51%.High Cd2+Under the conditions of (100mg/L), by high concentrations of Cd2+Influence, bacterial strain DY-1
Activity slightly reduce, but the removal efficiency of pollutant maintains essentially in higher level.
5 bacterial strain DY-1 of table contains Cd in difference2+Black and odorous water in activity and the removal effect to pollutant
Embodiment 8
Acidithiobacillus ferrooxidans strain GF DY-1 is containing Cr6+Black and odorous water in activity and the removal effect to pollutant：
Black smelly water sample picks up from Wuhan black-odor riverway used in experiment.Experimental provision is internal diameter 10cm, the cylinder of high 35cm
Container.Cr is passed through into reaction unit respectively6+Concentration is the black smelly water sample of 10mg/L and 100mg/L.3 repetitions of every group of processing,
It is placed at 28 DEG C, is aerated logical oxygen.The water sample of 12h, 24h, 48h, 72h period is taken to carry out Fe respectively2+Oxygenation efficiency and Cr6+、
S2-, organic matter and ammonia nitrogen removal rate analysis.
In low Cr6+Under conditions of (10mg/L), Fe2+Oxygenation efficiency maintain essentially in more than 90%, illustrate that DY-1 bacterium exist
Well-grown under 10mg/L concentration.Under this concentration, Cr6+Removal rate up to 95%, S2-Removal rate up to 97%, organic matter is gone
Except rate is 56%, ammonia nitrogen removal frank 51%.High Cr6+Under the conditions of (100mg/L), by high concentration Cr6+Influence, bacterial strain DY-1
Activity slightly reduce, but the removal efficiency of pollutant maintains essentially in higher level.
Activity of the 6 bacterial strain DY-1 of table in the black and odorous water of difference amount containing Cr and the removal effect to pollutant
Embodiment 9
Applications of the Acidithiobacillus ferrooxidans strain GF DY-1 in black-odor riverway improvement：
Acidithiobacillus ferrooxidans strain GF DY-1 provided by the invention is applied to Xuzhou river black-odor control demonstration project.
River bottom mud siltation is serious, sticks greasy black smelly and a large amount of useless containing largely harmful organic matter and heavy metal, water surface floating
Oil.Since water quality condition is severe, early period uses and adds Acidithiobacillus ferrooxidans strain GF DY-1 and artificial oxygenation of the present invention
The mode being combined, detoxifies black and odorous water and bed mud, is nursed one's health.Continuously add acidophilus ferrous oxide sulphur of the present invention
Black and odorous water phenomenon has obvious improvement in bacillus DY-1,5~8d or so demonstration area.Water body stench dissipates, and odor concentration is low
To 10, water transparency is improved to 50~60cm by original less than 20cm, starts algae and protozoan occur in water；It is black smelly
Bed mud gradually become odorless yellow mud, bed mud thickness cuts down 15~20cm.Subsequently to use other microorganisms, water plant etc.
The further river training water quality of administering method, water ecology balance of recovering have established good water quality and ecological microenvironment basis.
Table 7 adds the situation of the indices of black-odor riverway bed mud before and after bacterial strain DY-1
The invention is not limited to the above embodiment, any equivalent modifications carried out to the present invention and substitutes also all
Among scope of the invention.Therefore, the equivalent change made without departing from the spirit and scope of the invention and modification, all should
Cover within the scope of the present invention.
Claims (6)
1. one plant of separated Acidithiobacillus ferrooxidans strain GF, the Acidithiobacillus ferrooxidans strain GF is acidophilus ferrous oxide sulphur bar
Bacterium（Acidithiobacillus ferrooxidans ）DY-1, deposit number are：CCTCC NO：M2017806.
2. application of the Acidithiobacillus ferrooxidans strain GF described in claim 1 in environmental improvement.
3. application of the Acidithiobacillus ferrooxidans strain GF described in claim 1 in the cleanser of black and odorous water is prepared.
4. application of the Acidithiobacillus ferrooxidans strain GF in foul gas distintegrant is prepared described in claim 1.
5. the answering in the heavy metals removal agent in preparing black and odorous water of the Acidithiobacillus ferrooxidans strain GF described in claim 1
With.
6. application of the Acidithiobacillus ferrooxidans strain GF in the modifying agent of bed mud in preparing black and odorous water described in claim 1.
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| CN108728557A (en) * | 2018-05-28 | 2018-11-02 | 深圳大学 | Primer pair and design method for detecting thiobacillus ferrooxidans and detection method |
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| CN110342764A (en) * | 2019-06-06 | 2019-10-18 | 武汉二航路桥特种工程有限责任公司 | The agent of organic sludge rapid-digestion and processing method based on microorganism predation technology |
| CN111808773A (en) * | 2020-07-22 | 2020-10-23 | 东华理工大学 | Acidithiobacillus ferrooxidans and application thereof and ore leaching method |
| CN113548778A (en) * | 2021-08-19 | 2021-10-26 | 中交四航工程研究院有限公司 | Biological remediation system and method for heavy metal-organic composite polluted river sediment |
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| CN111808773A (en) * | 2020-07-22 | 2020-10-23 | 东华理工大学 | Acidithiobacillus ferrooxidans and application thereof and ore leaching method |
| CN113548778A (en) * | 2021-08-19 | 2021-10-26 | 中交四航工程研究院有限公司 | Biological remediation system and method for heavy metal-organic composite polluted river sediment |
| CN113548778B (en) * | 2021-08-19 | 2022-07-26 | 中交四航工程研究院有限公司 | Bioremediation method of heavy metal-organic composite polluted river sediment |
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