CN112375716B - Garlic slice processing wastewater deodorization composite microbial inoculum - Google Patents
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- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F3/00—Biological treatment of water, waste water, or sewage
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- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
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- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
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- C02F2101/00—Nature of the contaminant
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- C02F2101/40—Organic compounds containing sulfur
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- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F2303/00—Specific treatment goals
- C02F2303/02—Odour removal or prevention of malodour
Abstract
The invention relates to a deodorization composite microbial inoculum for garlic slice processing wastewater, which can effectively reduce the odor of the garlic slice processing wastewater and relieve the problem of environmental pollution, and the composite microbial inoculum is composed of a bacillus amyloliquefaciens WS3-1 microbial inoculum, a bacillus licheniformis WS3-2 microbial inoculum, a saccharomyces cerevisiae CXMJ microbial inoculum and a rhizopus oryzae JG3 microbial inoculum, wherein the volume ratio of the bacillus amyloliquefaciens WS3-1 microbial inoculum, the bacillus licheniformis WS3-2 microbial inoculum, the saccharomyces cerevisiae CXMJ microbial inoculum and the rhizopus oryzae JG3 microbial inoculum is 1:1: 1-2: 1-3; the effective viable count of the bacillus amyloliquefaciens WS3-1, the bacillus licheniformis WS3-2, the saccharomyces cerevisiae CXMJ and the rhizopus oryzae JG3 is more than 0.2 hundred million/mL, and the invention degrades the organic sulfides such as garlicin and the like to achieve the aim of deodorization, thereby being an innovation on a microbial preparation for wastewater treatment.
Description
Technical Field
The invention relates to the field of microorganisms, in particular to a deodorization composite microbial inoculum for garlic slice processing wastewater.
Background
The garlic slice processing wastewater is wastewater discharged in the garlic slice production process, the garlic yield is the top of the world in China, the garlic slice is mainly used for garlic processing, and the garlic processing comprises the steps of selecting, cleaning, slicing, rinsing, dehydrating and the like. In which a large amount of organic waste water is generated during cleaning, rinsing and spin-drying. It is estimated that 30-40 tons of water are consumed for every 1 ton of dehydrated garlic flakes. Because garlic contains alliin, alliinase is activated to form allicin when the garlic is physically extruded or crushed, and the allicin has a strong inhibiting effect on bacteria and fungi, so that the traditional biological water treatment process is difficult to degrade the garlic processing wastewater.
The main components of the garlic flake processing wastewater mainly comprise organic matters such as polysaccharide and lipid substances, and the organic matters have no toxicity. Although the garlic processing wastewater does not contain toxic substances, it contains a large amount of biodegradable organic substances. If the waste water is directly discharged into a water body without treatment, a large amount of dissolved oxygen in the water needs to be consumed, and the water body is anoxic, so that aquatic organisms die. Suspended matters in the wastewater sink to the bottom of the water, deteriorating the water quality. Organic sulfide which is rich in the garlic processing wastewater generates special odor and seriously pollutes the environment when the garlic processing wastewater is decomposed under the anaerobic condition.
Although the garlic processing wastewater can be treated by using a biochemical-activated sludge method, the garlic processing wastewater contains antibacterial substances such as garlicin and the like, so that the garlic processing wastewater has strong killing power on bacteria, the normal metabolism of microorganisms is damaged, the subsequent activated sludge treatment is seriously interfered, an activated sludge biological system is very easy to impact to cause the paralysis of the system, the efficiency and the level of industrial application of the system are difficult to achieve, in addition, the discharge amount of the system has larger change and the concentration of organic matters is high, and the difficulty in garlic wastewater treatment is further increased. Therefore, the high-concentration organic sulfide garlic wastewater must be pretreated to reduce the influence on a subsequent biological treatment unit.
The heating pretreatment method has a certain effect on removing organic sulfides such as garlicin and the like, but the effect cannot meet the actual production requirement, the iron-carbon micro-electrolysis method is widely applied to wastewater treatment engineering, and the iron-carbon micro-electrolysis method is researched and adopted to pretreat garlic slice wastewater in China, so that the pretreatment effect on wastewater is realized. However, the process is unstable and has poor reproducibility in the process of treating wastewater. Meanwhile, a large amount of iron and carbon, acid and alkali regulation and power cost of aeration are consumed, and good effects on the aspects of manpower and material resources cannot be achieved. At present, the on-site operation of certain domestic water treatment plants shows that the acid-base pretreatment-iron-carbon microelectrolysis-flocculation-combined pretreatment process has the effect of removing organic sulfides in the garlic wastewater. However, a large amount of dangerous chemicals such as acid reagents and alkali reagents are used in the process, so that the environment is greatly threatened, the pressure is brought to the subsequent biochemical treatment, and the water treatment cost is greatly increased due to the consumption of a large amount of chemical reagents, iron, carbon and other materials every year.
In the 20 th century and the 80 th era, Germany and the Netherlands and other countries utilize microorganisms to treat odor with good effect, which attracts the attention of the America, Japan and other countries, and currently, biological deodorization is a main technology for treating the odor. Nitrogen-containing compounds and sulfur-containing compounds are considered as the main malodorous substances, and therefore the kinds of deodorizing microorganisms are mainly: ammonia-removing microorganisms and desulfurization microorganisms. The microbial deodorization technique utilizes the metabolism of microbes to degrade malodorous components into odorless and harmless end products, such as H2O、CO2Etc. to achieve the purpose of deodorization.
The microbial deodorization technology has obvious deodorization effect on odor and low requirement on environmental conditions, the energy required by growth and reproduction is oxidation energy generated in the oxidative decomposition process, other nutrient substances are not needed, and the advantages of low operation cost, high efficiency, no secondary pollution, simple required equipment, convenient management and maintenance and the like are the main technology for treating the odor. The operation cost is low. The physical and chemical deodorization technologies have the defects of complex equipment and process, high cost, secondary pollution, high energy consumption and the like, so the microbial deodorization technology is widely applied to garbage deodorization and sewage treatment at present.
The garlic sewage contains a large amount of Allicin which is called diallyl thiosulfinate in the chemical name, is an organic sulfur compound generated by converting Alliin (Alliin) in the garlic under the action of Alliinase (Alliinase), and is one of main functional components in the garlic. The active sulfur (thio group and thioether group) in the allicin has redox property, can remove oxygen free radical, endow the allicin with antioxidant activity, can react with sulfhydryl group in bacterial cells, and endow the allicin with broad-spectrum antibacterial activity.
The waste water produced in the garlic product processing process not only has pungent garlic odor and higher Chemical Oxygen Demand (COD), but also the garlicin in the waste water can inhibit the growth of microorganisms in activated sludge, and becomes one of the current problems in waste water treatment. At present, studies on physiological activity, a sterilization mechanism, extraction and separation and chemical synthesis of allicin are more, but the studies on a biodegradation mechanism are few, and the separation of allicin degrading bacteria is not reported, so that the biological treatment of allicin processing waste is directly influenced, and how to degrade the allicin by using microorganisms becomes a hotspot of the current allicin processing wastewater treatment study.
Because the components of the garlic flake processing wastewater are very complex, and microbial deodorization is a complex biochemical process, the generation of malodorous substances can be better inhibited only by forming a dominant mixed flora through the mutualistic symbiosis of various bacteria. Therefore, how to solve the problem of treating the garlic flake processing wastewater is a technical problem which is urgently needed to be solved at present.
Disclosure of Invention
In order to solve the defects of the prior art, the invention aims to provide the deodorizing composite microbial inoculum for the garlic slice processing wastewater, which can effectively reduce the odor of the garlic slice processing wastewater and relieve the problem of environmental pollution.
The technical scheme includes that the composite microbial inoculum consists of a bacillus amyloliquefaciens WS3-1 microbial inoculum, a bacillus licheniformis WS3-2 microbial inoculum, a saccharomyces cerevisiae CXMJ microbial inoculum and a rhizopus oryzae JG3 microbial inoculum, wherein the volume ratio of the bacillus amyloliquefaciens WS3-1 microbial inoculum, the bacillus licheniformis WS3-2 microbial inoculum, the saccharomyces cerevisiae CXMJ microbial inoculum and the rhizopus oryzae JG3 microbial inoculum is 1:1: 1-2: 1-3; wherein the effective viable count of the bacillus amyloliquefaciens WS3-1, the bacillus licheniformis WS3-2, the saccharomyces cerevisiae CXMJ and the rhizopus oryzae JG3 is more than 0.2 hundred million/mL.
The bacillus amyloliquefaciens WS3-1 microbial inoculum is WS3-1 spore bacterial liquid, specifically, WS3-1 slant strain is selected and inoculated in a liquid LB culture medium, shaking culture is carried out at 37 ℃ and 180r/min for 24h to obtain seed liquid, the seed liquid is inoculated in the WS3-1 spore production culture medium according to the inoculum size of 1-3% in volume ratio, shaking culture is carried out at 37 ℃ and 180r/min for 48h to obtain WS3-1 spore bacterial liquid, and the spore formation rate reaches 100%; the WS3-1 sporulation culture medium: 30g of glucose, 40g of yeast powder, 2g of magnesium sulfate heptahydrate, 2g of dipotassium phosphate, 1000mL of distilled water, 7.0-7.5 of pH value and 20min of sterilization at 115 ℃.
The bacillus licheniformis WS3-2 microbial inoculum is WS3-2 spore bacterial liquid, specifically, WS3-2 slant strain is selected and inoculated in a liquid LB culture medium, shaking table culture is carried out at 37 ℃ and 180r/min for 12-24 h to obtain seed liquid, the seed liquid is inoculated in the WS3-2 spore production culture medium according to the inoculum size of 1-3% in volume ratio, shaking table culture is carried out at 37 ℃ and 180r/min for 48h to obtain WS3-2 spore bacterial liquid, and the spore formation rate is more than 98%; the WS3-2 spore production culture medium comprises the following components in percentage by mass: 1% soybean meal, 0.5% tryptone, 0.5% NaCl, 0.03% FeCl3, pH 7.5.
The saccharomyces cerevisiae CXMJ microbial inoculum is CXMJ zymocyte liquid, and specifically comprises the steps of inoculating a slant strain saccharomyces cerevisiae CXMJ on an YPD plate, culturing at 25-30 ℃ for 2-3 d, selecting a larger colony, inoculating in an YPD liquid culture medium, and culturing at 25-30 ℃ for 16-24 h to obtain a pre-culture solution; then taking a proper amount of yeast pre-culture solution, inoculating the yeast pre-culture solution into a fresh sterile YPD liquid culture medium, and carrying out shaking culture on the culture solution at 30 ℃ and 150r/min for 16-24 h to obtain a fermentation seed solution; inoculating the seed solution into a fermentation culture medium according to the inoculation amount of 1-3% of the volume ratio, and performing shake culture at 25-30 ℃ at 180r/min for 2-3 d to obtain a zymophyte solution; the YPD culture medium comprises: yeast powder 10.0g, tryptone 20.0g, glucose 20.0g, adding into distilled water 1000mL (pH natural), sterilizing at 115 deg.C for 20 min; the fermentation medium comprises: 20g of glucose, 7.5g of peptone, 7.5g of yeast powder, 0.156g of monopotassium phosphate and 0.496g of magnesium sulfate heptahydrate are added into 1000mL of distilled water (pH is natural), and the mixture is sterilized at 115 ℃ for 20 min.
The rhizopus oryzae JG3 microbial inoculum is JG3 zymogen liquid, and specifically comprises the steps of inoculating a slant strain rhizopus oryzae JG3 on a PDA culture medium flat plate, culturing for 2-3 d at 25-30 ℃, taking 3-5 bacterial blocks by using a puncher, inoculating into a PDA culture medium, and performing shake culture at 25-30 ℃ for 180r/min for 24-36 h to obtain a seed liquid; inoculating the seed liquid into a fermentation culture medium according to the inoculation amount of 1-3% of the volume ratio, and performing shake cultivation at the temperature of 25-30 ℃ and 180r/min for 2-3 d to obtain a zymogen liquid.
The Bacillus amyloliquefaciens WS3-1 is classified and named as Bacillus amyloliquefaciens (Bacillus amyloliquefaciens), and is preserved in China general microbiological culture Collection center (CGMCC) in 11-26 th of 2019 with the preservation number of CGMCC NO19013 and the preservation address: xilu No.1 Hospital No. 3, Beijing, Chaoyang, Beicheng.
The Bacillus licheniformis WS3-2 is classified and named as Bacillus licheniformis (Bacillus licheniformis), and has been preserved in China general microbiological culture Collection center in 11 months and 26 days in 2019, with the preservation number of CGMCC NO19012 and the preservation address of: xilu No.1 Hospital No. 3, Beijing, Chaoyang, Beicheng.
The Saccharomyces cerevisiae CXMJ is classified and named as Saccharomyces cerevisiae (Saccharomyces cerevisiae), is preserved in China general microbiological culture Collection center (CGMCC) in 11 and 26 months in 2019, has a preservation number of CGMCC NO19011, and has a preservation address: xilu No.1 Hospital No. 3, Beijing, Chaoyang, Beicheng.
The Rhizopus oryzae JG3 is classified and named as Rhizopus oryzae (Rhizopus oryzae), is preserved in China general microbiological culture Collection center in 11 months and 28 days in 2019, has the preservation number of CGMCC NO19028, and has the preservation address: xilu No.1 Hospital No. 3, Beijing, Chaoyang, North.
The deodorization composite microbial inoculum for garlic slice processing sewage provided by the invention can be applied to pretreatment of garlic processing wastewater degradation treatment and degradation of organic sulfides such as garlicin and the like so as to achieve the aim of deodorization, and is an innovation in a wastewater treatment microbial preparation.
Detailed Description
The following examples further illustrate the embodiments of the present invention in detail.
Example 1
In specific implementation, the composite microbial inoculum consists of a bacillus amyloliquefaciens WS3-1 microbial inoculum, a bacillus licheniformis WS3-2 microbial inoculum, a saccharomyces cerevisiae CXMJ microbial inoculum and a rhizopus oryzae JG3 microbial inoculum according to a volume ratio of 1:1:1:1, wherein the effective viable count of the bacillus amyloliquefaciens WS3-1, the bacillus licheniformis WS3-2, the saccharomyces cerevisiae CXMJ and the rhizopus oryzae JG3 is more than 0.2 hundred million/mL.
Example 2
In the specific implementation of the invention, the composite microbial inoculum consists of a bacillus amyloliquefaciens WS3-1 microbial inoculum, a bacillus licheniformis WS3-2 microbial inoculum, a saccharomyces cerevisiae CXMJ microbial inoculum and a rhizopus oryzae JG3 microbial inoculum according to the volume ratio of 1:1:2:2, wherein the effective viable count of the bacillus amyloliquefaciens WS3-1, the bacillus licheniformis WS3-2, the saccharomyces cerevisiae CXMJ and the rhizopus oryzae JG3 is more than 0.2 hundred million/mL.
Example 3
In the specific implementation of the invention, the composite microbial inoculum consists of a bacillus amyloliquefaciens WS3-1 microbial inoculum, a bacillus licheniformis WS3-2 microbial inoculum, a saccharomyces cerevisiae CXMJ microbial inoculum and a rhizopus oryzae JG3 microbial inoculum according to the volume ratio of 1:1:2:3, wherein the effective viable count of the bacillus amyloliquefaciens WS3-1, the bacillus licheniformis WS3-2, the saccharomyces cerevisiae CXMJ and the rhizopus oryzae JG3 is more than 0.2 hundred million/mL.
Example 4
In the specific implementation of the invention, the composite microbial inoculum consists of a bacillus amyloliquefaciens WS3-1 microbial inoculum, a bacillus licheniformis WS3-2 microbial inoculum, a saccharomyces cerevisiae CXMJ microbial inoculum and a rhizopus oryzae JG3 microbial inoculum according to the volume ratio of 1:1:1:3, wherein the effective viable count of the bacillus amyloliquefaciens WS3-1, the bacillus licheniformis WS3-2, the saccharomyces cerevisiae CXMJ and the rhizopus oryzae JG3 is more than 0.2 hundred million/mL.
Example 5
In the specific implementation of the invention, the composite microbial inoculum consists of a bacillus amyloliquefaciens WS3-1 microbial inoculum, a bacillus licheniformis WS3-2 microbial inoculum, a saccharomyces cerevisiae CXMJ microbial inoculum and a rhizopus oryzae JG3 microbial inoculum according to the volume ratio of 1:1:2:1, wherein the effective viable count of the bacillus amyloliquefaciens WS3-1, the bacillus licheniformis WS3-2, the saccharomyces cerevisiae CXMJ and the rhizopus oryzae JG3 is more than 0.2 hundred million/mL.
The compound microbial inoculum prepared by the invention can grow for a long time in an allicin stress environment, can achieve the long-time deodorization effect, and the test data of the deodorization effect are as follows:
1. respectively adding four single-bacterium inoculants, namely 10mL of liquid deodorization composite inoculum prepared in the embodiment 1 of the invention and Bacillus amyloliquefaciens WS3-1 inoculum, Bacillus licheniformis WS3-2 inoculum, Saccharomyces cerevisiae CXMJ inoculum and Rhizopus oryzae JG3 inoculum, into a beaker (1L) filled with 100mL of garlic slice processing sewage, and sealing the beaker by using a preservative film; at the same time, the control without adding the fungicide is carried out, three times are set for each group, the degradation rate of hydrogen sulfide and the degradation rate of allicin are measured every day, and the results of continuous 5-day measurement are shown in tables 1 and 2.
The measurement results in table 1 show that after the deodorization composite microbial inoculum is treated by each microbial inoculum for 5 days, the degradation rate of the hydrogen sulfide is 86.46% at most compared with the control group, and the degradation rate difference of the hydrogen sulfide is extremely obvious compared with other single microbial inoculum treatment groups (P is less than 0.01). The determination results in table 2 show that, after the treatment for 2 days by using each microbial inoculum, the degradation rate of the deodorization composite microbial inoculum is up to 100% compared with the control, namely, the allicin in the garlic slice processing sewage is completely degraded, and the allicin in other single microbial inoculum treatment groups is not completely degraded even in the 5 th day.
The hydrogen sulfide degradation rate (%) is [ (hydrogen sulfide content in a control group-hydrogen sulfide content after treatment with a fungicide) ÷ hydrogen sulfide content in a control group ] × 100%;
the degradation rate (%) of the allicin is [ (allicin content in a control group-allicin content after being treated by a microbial inoculum) ÷ allicin content in a control group ] × 100%;
TABLE 1 determination result of hydrogen sulfide degradation rate in deodorization effect test of garlic slice processing sewage deodorization composite bacteria agent
TABLE 2 determination results of degradation rate of allicin by deodorizing effect test of garlic slice processing sewage deodorizing composite bacterial agent
In addition to the above tests, the same or similar results were obtained by performing the same tests using the deodorizing complex microbial agents of examples 2 to 5, which are not listed here.
Therefore, the deodorization composite bacterial agent provided by the invention can be used for remarkably degrading hydrogen sulfide gas and allicin in the garlic slice processing sewage so as to achieve the purpose of remarkable deodorization, and compared with the existing physical chemistry and biological deodorization technologies, the microorganism composite bacterial agent provided by the invention has no secondary pollution, the deodorization effect of the composite bacterial agent is remarkably higher than that of a single strain, and especially the composite bacterial agent can be used for efficiently removing allicin and hydrogen sulfide; the microbial agent can grow for a long time in an allicin stress environment, and can achieve the effect of long-time deodorization. The pretreated wastewater enters an activated sludge treatment system, and standard discharge can be realized. In addition, the fermentation medium and the culture conditions provided by the invention are simple, the operation is convenient, the industrial production is easy, the production cost is low, the invention is an innovation on a microbial preparation for wastewater treatment, and the invention has good economic and social benefits.
Claims (6)
1. The deodorization composite microbial inoculum for the garlic flake processing wastewater is characterized by comprising a bacillus amyloliquefaciens WS3-1 microbial inoculum, a bacillus licheniformis WS3-2 microbial inoculum, a saccharomyces cerevisiae CXMJ microbial inoculum and a rhizopus oryzae JG3 microbial inoculum, wherein the volume ratio of the bacillus amyloliquefaciens WS3-1 microbial inoculum, the bacillus licheniformis WS3-2 microbial inoculum, the saccharomyces cerevisiae CXMJ microbial inoculum and the rhizopus oryzae JG3 microbial inoculum is 1:1: 1-2: 1-3; wherein the effective viable count of the bacillus amyloliquefaciens WS3-1, the bacillus licheniformis WS3-2, the saccharomyces cerevisiae CXMJ and the rhizopus oryzae JG3 is more than 0.2 hundred million/mL;
the bacillus amyloliquefaciens WS3-1 microbial inoculum is WS3-1 spore bacterial liquid, specifically, WS3-1 slant strain is selected and inoculated in a liquid LB culture medium, shaking culture is carried out at 37 ℃ and 180r/min for 24h to obtain seed liquid, the seed liquid is inoculated in the WS3-1 spore production culture medium according to the inoculum size of 1-3% in volume ratio, shaking culture is carried out at 37 ℃ and 180r/min for 48h to obtain WS3-1 spore bacterial liquid, and the spore formation rate reaches 100%; the WS3-1 sporulation culture medium: 30g of glucose, 40g of yeast powder, 2g of magnesium sulfate heptahydrate, 2g of dipotassium phosphate, adding distilled water to 1000mL, adjusting the pH value to 7.0-7.5, and sterilizing at 115 ℃ for 20 min;
the bacillus licheniformis WS3-2 microbial inoculum is WS3-2 spore bacterial liquid, specifically, WS3-2 slant strain is selected and inoculated in a liquid LB culture medium, shaking table culture is carried out at 37 ℃ and 180r/min for 12-24 h to obtain seed liquid, the seed liquid is inoculated in the WS3-2 spore production culture medium according to the inoculum size of 1-3% in volume ratio, shaking table culture is carried out at 37 ℃ and 180r/min for 48h to obtain WS3-2 spore bacterial liquid, and the spore formation rate is more than 98%; the WS3-2 spore production culture medium comprises the following components in percentage by mass: 1% of soybean meal, 0.5% of tryptone, 0.5% of NaCl, 0.03% of FeCl3，pH 7.5；
The saccharomyces cerevisiae CXMJ microbial inoculum is CXMJ zymogen liquid, and specifically comprises the steps of inoculating a slant strain saccharomyces cerevisiae CXMJ on an YPD flat plate, culturing at 25-30 ℃ for 2-3 d, selecting a larger colony to inoculate in an YPD liquid culture medium, and culturing at 25-30 ℃ for 16-24 h to obtain a pre-culture solution; then taking a proper amount of yeast pre-culture solution, inoculating the yeast pre-culture solution into a fresh sterile YPD liquid culture medium, and carrying out shaking culture on the culture solution at 30 ℃ and 150r/min for 16-24 h to obtain a fermentation seed solution; inoculating the seed liquid into a fermentation culture medium according to the inoculation amount of 1-3% of the volume ratio, and performing shake culture at 25-30 ℃ for 2-3 d at 180r/min to obtain a zymogen liquid; the YPD culture medium comprises: yeast powder 10.0g, tryptone 20.0g, glucose 20.0g, adding into distilled water 1000mL, sterilizing at 115 deg.C for 20 min; the fermentation medium comprises: 20g of glucose, 7.5g of peptone, 7.5g of yeast powder, 0.156g of monopotassium phosphate and 0.496g of magnesium sulfate heptahydrate, adding 1000mL of distilled water, and sterilizing at 115 ℃ for 20 min;
the rhizopus oryzae JG3 microbial inoculum is JG3 zymogen liquid, and specifically comprises the steps of inoculating a slant strain rhizopus oryzae JG3 on a PDA culture medium flat plate, culturing for 2-3 d at 25-30 ℃, taking 3-5 bacterial blocks by using a puncher, inoculating into a PDA culture medium, and performing shake culture at 25-30 ℃ for 180r/min for 24-36 h to obtain a seed liquid; inoculating the seed liquid into a fermentation culture medium according to the inoculation amount of 1-3% of the volume ratio, and performing shake culture at 25-30 ℃ for 2-3 d at 180r/min to obtain a zymogen liquid;
the bacillus amyloliquefaciens WS3-1 is classified and named as bacillus amyloliquefaciens (A)Bacillus amyloliquefaciens) The microbial inoculum is preserved in China general microbiological culture Collection center (CGMCC) at 26.11.2019, and the preservation number is CGMCC No. 19013;
the Bacillus licheniformis WS3-2 is classified and named as Bacillus licheniformis (b)Bacillus licheniformis) The strain has been preserved in China general microbiological culture Collection center (CGMCC) in 26.11.2019 with the preservation number of CGMCC No. 19012;
the saccharomyces cerevisiae CXMJ is classified and named as saccharomyces cerevisiae (C) ((C))Saccharomyces cerevisiae) The strain has been preserved in China general microbiological culture Collection center (CGMCC) in 26.11.2019 with the preservation number of CGMCC No. 19011;
the rhizopus oryzae JG3 is named as rhizopus oryzae (A), (B), (C) and (C)Rhizopus oryzae) The strain has been preserved in China general microbiological culture Collection center (CGMCC) at 28.11.2019 with the preservation number of CGMCC No. 19028.
2. The deodorization composite microbial inoculum for garlic slice processing wastewater as claimed in claim 1, which is composed of bacillus amyloliquefaciens WS3-1 microbial inoculum, bacillus licheniformis WS3-2 microbial inoculum, saccharomyces cerevisiae CXMJ microbial inoculum and rhizopus oryzae JG3 microbial inoculum according to the volume ratio of 1:1:1: 1.
3. The deodorization composite microbial inoculum for the garlic slice processing wastewater as claimed in claim 1, which is composed of a bacillus amyloliquefaciens WS3-1 microbial inoculum, a bacillus licheniformis WS3-2 microbial inoculum, a saccharomyces cerevisiae CXMJ microbial inoculum and a rhizopus oryzae JG3 microbial inoculum according to a volume ratio of 1:1:2: 2.
4. The deodorization composite microbial inoculum for the garlic slice processing wastewater as claimed in claim 1, which is composed of a bacillus amyloliquefaciens WS3-1 microbial inoculum, a bacillus licheniformis WS3-2 microbial inoculum, a saccharomyces cerevisiae CXMJ microbial inoculum and a rhizopus oryzae JG3 microbial inoculum according to a volume ratio of 1:1:2: 3.
5. The deodorization composite microbial inoculum for garlic slice processing wastewater as claimed in claim 1, which is composed of bacillus amyloliquefaciens WS3-1 microbial inoculum, bacillus licheniformis WS3-2 microbial inoculum, saccharomyces cerevisiae CXMJ microbial inoculum and rhizopus oryzae JG3 microbial inoculum according to the volume ratio of 1:1:1: 3.
6. The deodorization composite microbial inoculum for the garlic slice processing wastewater as claimed in claim 1, which is composed of a bacillus amyloliquefaciens WS3-1 microbial inoculum, a bacillus licheniformis WS3-2 microbial inoculum, a saccharomyces cerevisiae CXMJ microbial inoculum and a rhizopus oryzae JG3 microbial inoculum according to a volume ratio of 1:1:2: 1.
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