CN110358699A - A kind of complex microorganism deodorizing microorganism and the preparation method and application thereof - Google Patents
A kind of complex microorganism deodorizing microorganism and the preparation method and application thereof Download PDFInfo
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- 244000005700 microbiome Species 0.000 title claims abstract description 86
- 230000001877 deodorizing effect Effects 0.000 title claims abstract description 51
- 238000002360 preparation method Methods 0.000 title claims abstract description 12
- 241000186605 Lactobacillus paracasei Species 0.000 claims abstract description 16
- 238000004332 deodorization Methods 0.000 claims abstract description 11
- 230000001580 bacterial effect Effects 0.000 claims abstract description 9
- 241000235648 Pichia Species 0.000 claims abstract description 8
- 241000235645 Pichia kudriavzevii Species 0.000 claims abstract description 7
- 241000894006 Bacteria Species 0.000 claims description 23
- 239000002609 medium Substances 0.000 claims description 18
- 238000005457 optimization Methods 0.000 claims description 15
- WQZGKKKJIJFFOK-GASJEMHNSA-N Glucose Natural products OC[C@H]1OC(O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-GASJEMHNSA-N 0.000 claims description 12
- 229940041514 candida albicans extract Drugs 0.000 claims description 12
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 12
- 239000012138 yeast extract Substances 0.000 claims description 12
- 239000008103 glucose Substances 0.000 claims description 11
- 239000012530 fluid Substances 0.000 claims description 9
- 239000002054 inoculum Substances 0.000 claims description 9
- 235000015097 nutrients Nutrition 0.000 claims description 9
- CSNNHWWHGAXBCP-UHFFFAOYSA-L Magnesium sulfate Chemical compound [Mg+2].[O-][S+2]([O-])([O-])[O-] CSNNHWWHGAXBCP-UHFFFAOYSA-L 0.000 claims description 8
- 239000007788 liquid Substances 0.000 claims description 8
- 239000008399 tap water Substances 0.000 claims description 8
- 235000020679 tap water Nutrition 0.000 claims description 8
- 239000012137 tryptone Substances 0.000 claims description 8
- 239000001963 growth medium Substances 0.000 claims description 7
- 239000007789 gas Substances 0.000 claims description 6
- 239000001888 Peptone Substances 0.000 claims description 5
- 108010080698 Peptones Proteins 0.000 claims description 5
- 235000019319 peptone Nutrition 0.000 claims description 5
- 230000003519 ventilatory effect Effects 0.000 claims description 5
- VMHLLURERBWHNL-UHFFFAOYSA-M Sodium acetate Chemical compound [Na+].CC([O-])=O VMHLLURERBWHNL-UHFFFAOYSA-M 0.000 claims description 4
- 229910052943 magnesium sulfate Inorganic materials 0.000 claims description 4
- 235000019341 magnesium sulphate Nutrition 0.000 claims description 4
- 238000000034 method Methods 0.000 claims description 4
- 239000001632 sodium acetate Substances 0.000 claims description 4
- 235000017281 sodium acetate Nutrition 0.000 claims description 4
- 238000002425 crystallisation Methods 0.000 claims description 3
- 230000008025 crystallization Effects 0.000 claims description 3
- ZPWVASYFFYYZEW-UHFFFAOYSA-L dipotassium hydrogen phosphate Chemical compound [K+].[K+].OP([O-])([O-])=O ZPWVASYFFYYZEW-UHFFFAOYSA-L 0.000 claims description 3
- 238000011534 incubation Methods 0.000 claims description 3
- 239000002994 raw material Substances 0.000 claims description 3
- 230000003068 static effect Effects 0.000 claims description 3
- 150000001875 compounds Chemical class 0.000 claims description 2
- 239000002068 microbial inoculum Substances 0.000 claims description 2
- 238000002156 mixing Methods 0.000 claims description 2
- 229940057059 monascus purpureus Drugs 0.000 claims 1
- 238000000855 fermentation Methods 0.000 abstract description 6
- 230000004151 fermentation Effects 0.000 abstract description 6
- 244000144977 poultry Species 0.000 abstract description 5
- 244000144972 livestock Species 0.000 abstract description 4
- 238000012360 testing method Methods 0.000 abstract description 3
- 238000005516 engineering process Methods 0.000 abstract description 2
- 230000002110 toxicologic effect Effects 0.000 abstract description 2
- 231100000027 toxicology Toxicity 0.000 abstract description 2
- 239000006041 probiotic Substances 0.000 abstract 1
- 235000018291 probiotics Nutrition 0.000 abstract 1
- QGZKDVFQNNGYKY-UHFFFAOYSA-N Ammonia Chemical compound N QGZKDVFQNNGYKY-UHFFFAOYSA-N 0.000 description 18
- 229910021529 ammonia Inorganic materials 0.000 description 9
- 239000000243 solution Substances 0.000 description 8
- RWSOTUBLDIXVET-UHFFFAOYSA-N Dihydrogen sulfide Chemical compound S RWSOTUBLDIXVET-UHFFFAOYSA-N 0.000 description 7
- 239000002781 deodorant agent Substances 0.000 description 7
- 229910000037 hydrogen sulfide Inorganic materials 0.000 description 7
- 230000000813 microbial effect Effects 0.000 description 5
- 239000007921 spray Substances 0.000 description 4
- 239000000126 substance Substances 0.000 description 4
- LSDPWZHWYPCBBB-UHFFFAOYSA-N Methanethiol Chemical compound SC LSDPWZHWYPCBBB-UHFFFAOYSA-N 0.000 description 3
- 239000003795 chemical substances by application Substances 0.000 description 3
- KRKNYBCHXYNGOX-UHFFFAOYSA-N citric acid Chemical compound OC(=O)CC(O)(C(O)=O)CC(O)=O KRKNYBCHXYNGOX-UHFFFAOYSA-N 0.000 description 3
- 230000007613 environmental effect Effects 0.000 description 3
- DNJIEGIFACGWOD-UHFFFAOYSA-N ethanethiol Chemical compound CCS DNJIEGIFACGWOD-UHFFFAOYSA-N 0.000 description 3
- 239000001257 hydrogen Substances 0.000 description 3
- 229910052739 hydrogen Inorganic materials 0.000 description 3
- 238000005259 measurement Methods 0.000 description 3
- 235000010482 polyoxyethylene sorbitan monooleate Nutrition 0.000 description 3
- 229920000053 polysorbate 80 Polymers 0.000 description 3
- UFHFLCQGNIYNRP-UHFFFAOYSA-N Hydrogen Chemical compound [H][H] UFHFLCQGNIYNRP-UHFFFAOYSA-N 0.000 description 2
- 239000003463 adsorbent Substances 0.000 description 2
- 239000012895 dilution Substances 0.000 description 2
- 238000010790 dilution Methods 0.000 description 2
- 239000004615 ingredient Substances 0.000 description 2
- 244000005706 microflora Species 0.000 description 2
- 238000004321 preservation Methods 0.000 description 2
- 238000011160 research Methods 0.000 description 2
- 238000007789 sealing Methods 0.000 description 2
- 238000004073 vulcanization Methods 0.000 description 2
- QGZKDVFQNNGYKY-UHFFFAOYSA-O Ammonium Chemical compound [NH4+] QGZKDVFQNNGYKY-UHFFFAOYSA-O 0.000 description 1
- 241000193830 Bacillus <bacterium> Species 0.000 description 1
- 108090000790 Enzymes Proteins 0.000 description 1
- 102000004190 Enzymes Human genes 0.000 description 1
- 241000287828 Gallus gallus Species 0.000 description 1
- 241000235058 Komagataella pastoris Species 0.000 description 1
- 241001465754 Metazoa Species 0.000 description 1
- 208000027418 Wounds and injury Diseases 0.000 description 1
- 229910000147 aluminium phosphate Inorganic materials 0.000 description 1
- 235000015278 beef Nutrition 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 238000009395 breeding Methods 0.000 description 1
- 230000001488 breeding effect Effects 0.000 description 1
- 239000000969 carrier Substances 0.000 description 1
- 230000015556 catabolic process Effects 0.000 description 1
- 235000013351 cheese Nutrition 0.000 description 1
- 239000002361 compost Substances 0.000 description 1
- 238000011109 contamination Methods 0.000 description 1
- 239000006071 cream Substances 0.000 description 1
- 230000006378 damage Effects 0.000 description 1
- 238000006731 degradation reaction Methods 0.000 description 1
- 238000011161 development Methods 0.000 description 1
- 238000007865 diluting Methods 0.000 description 1
- KCIDZIIHRGYJAE-YGFYJFDDSA-L dipotassium;[(2r,3r,4s,5r,6r)-3,4,5-trihydroxy-6-(hydroxymethyl)oxan-2-yl] phosphate Chemical compound [K+].[K+].OC[C@H]1O[C@H](OP([O-])([O-])=O)[C@H](O)[C@@H](O)[C@H]1O KCIDZIIHRGYJAE-YGFYJFDDSA-L 0.000 description 1
- 235000013399 edible fruits Nutrition 0.000 description 1
- 230000000694 effects Effects 0.000 description 1
- 230000008030 elimination Effects 0.000 description 1
- 238000003379 elimination reaction Methods 0.000 description 1
- 238000005265 energy consumption Methods 0.000 description 1
- 239000003344 environmental pollutant Substances 0.000 description 1
- 238000002474 experimental method Methods 0.000 description 1
- 210000003608 fece Anatomy 0.000 description 1
- 230000005764 inhibitory process Effects 0.000 description 1
- 208000014674 injury Diseases 0.000 description 1
- 239000010871 livestock manure Substances 0.000 description 1
- 230000005923 long-lasting effect Effects 0.000 description 1
- 229940099596 manganese sulfate Drugs 0.000 description 1
- 235000007079 manganese sulphate Nutrition 0.000 description 1
- 239000011702 manganese sulphate Substances 0.000 description 1
- SQQMAOCOWKFBNP-UHFFFAOYSA-L manganese(II) sulfate Chemical compound [Mn+2].[O-]S([O-])(=O)=O SQQMAOCOWKFBNP-UHFFFAOYSA-L 0.000 description 1
- 238000004519 manufacturing process Methods 0.000 description 1
- 239000005416 organic matter Substances 0.000 description 1
- 230000000505 pernicious effect Effects 0.000 description 1
- NBIIXXVUZAFLBC-UHFFFAOYSA-N phosphoric acid Substances OP(O)(O)=O NBIIXXVUZAFLBC-UHFFFAOYSA-N 0.000 description 1
- -1 phosphoric acid hydrogen Chemical class 0.000 description 1
- 238000000053 physical method Methods 0.000 description 1
- 239000002985 plastic film Substances 0.000 description 1
- 229920006255 plastic film Polymers 0.000 description 1
- 231100000719 pollutant Toxicity 0.000 description 1
- 235000013406 prebiotics Nutrition 0.000 description 1
- 230000002265 prevention Effects 0.000 description 1
- 238000012545 processing Methods 0.000 description 1
- 229920006395 saturated elastomer Polymers 0.000 description 1
- 238000012216 screening Methods 0.000 description 1
- 230000002195 synergetic effect Effects 0.000 description 1
- 238000012546 transfer Methods 0.000 description 1
Classifications
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B09—DISPOSAL OF SOLID WASTE; RECLAMATION OF CONTAMINATED SOIL
- B09B—DISPOSAL OF SOLID WASTE NOT OTHERWISE PROVIDED FOR
- B09B3/00—Destroying solid waste or transforming solid waste into something useful or harmless
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/14—Fungi; Culture media therefor
- C12N1/16—Yeasts; Culture media therefor
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/20—Bacteria; Culture media therefor
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A50/00—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE in human health protection, e.g. against extreme weather
- Y02A50/20—Air quality improvement or preservation, e.g. vehicle emission control or emission reduction by using catalytic converters
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Engineering & Computer Science (AREA)
- Health & Medical Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Biotechnology (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Genetics & Genomics (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Biomedical Technology (AREA)
- Virology (AREA)
- Microbiology (AREA)
- Tropical Medicine & Parasitology (AREA)
- Medicinal Chemistry (AREA)
- Biochemistry (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Mycology (AREA)
- Environmental & Geological Engineering (AREA)
- Botany (AREA)
- Disinfection, Sterilisation Or Deodorisation Of Air (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
Abstract
The present invention relates to the fields such as microbiology, Fermentation Engineering and deodorization technology, and in particular to a kind of complex microorganism deodorizing microorganism for places such as livestock and poultry farm, destructor plants.The lactobacillus paracasei (Lactobacillus paracasei) that complex microorganism deodorizing microorganism includes the pichia kudriavzevii (Pichia kudriavzevil) that deposit number is CGMCC No.16160 and deposit number is CGMCC No.16161, also discloses the preparation method and application of complex microorganism deodorizing microorganism.The contained bacterial strain of complex microorganism deodorizing microorganism of the invention is highly-safe, is the probiotics for exempting to do toxicological test, all safer no matter for user or applying environment.
Description
Technical field
The present invention relates to the fields such as microbiology, Fermentation Engineering and deodorization technology, and in particular to one kind is used for livestock and poultry cultivation
The complex microorganism deodorizing microorganism in the places such as field, destructor plant.
Background technique
Social economy's high speed development bring environmental problem is got worse, especially garbage disposal problem.Livestock and poultry farm,
The rubbish in the places such as refuse landfill can generate a large amount of stinks.Rubbish generate odour in have mainly have ammonia, hydrogen sulfide,
The pernicious gases ingredient such as mercaptan and methyl mercaptan.These foul gas can not only cause environment seriously to pollute, but also grievous injury
Human health.Therefore implement odor pollution control and management, the research for carrying out removing foul gas is of great significance.
Deodorant is broadly divided into physical deodorization agent, chemical deodorizing agent, microbial deodorant etc..Physical method will by adsorbent
Stink substances is absorbed into more empty carriers, and adsorbent is easily saturated and is difficult to desorb, and causes the secondary pollution of environment；Chemical deodorizing
Agent has the characteristics that quick, but its is with strong points, and when it encounters complicated foul gas ingredient, deodorizing effect can be substantially
It reduces；Microbial deodorant is to carry out directly absorbing degradation to odor pollutant using external source function strain or biological enzyme or dislike to producing
The inhibition of smelly microorganism removes stench, has many advantages, such as low energy consumption, environmental protection, it has also become domestic and international odor prevention research and application
In hot spot.
But it is the generally existing strain poor quality of microbial deodorant product currently on the market, living bacteria count virtual height, blind
Mesh compounds the problems such as strain, causes product using effect unstable.Therefore, the microbial deoderizer of efficient stable is researched and developed to solution
Certainly environmental hazard, elimination human health hidden danger are of great significance.
Summary of the invention
The object of the present invention is to provide one kind to be greatly improved deodorizing effect, and without subsequent contamination, without chemical residue
, the novel microbial deodorizing microorganism preparation method that bacterial strain is excellent, properties of product are stable.
The technical solution that the present invention takes is as follows:
A kind of complex microorganism deodorizing microorganism, comprising G1A2 and two kinds of effective bacterium of G2B5, the G1A2 is library Delhi Ah hereby
Prestige Pichia pastoris (Pichia kudriavzevil), deposit number are CGMCC No.16160；The G2B5 is secondary cheese cream
Bacillus (Lactobacillus paracasei), deposit number are CGMCC No.16161.
In complex microorganism deodorizing microorganism of the invention, the pichia kudriavzevii (Pichia
Kudriavzevil) and the bacterium solution ratio of lactobacillus paracasei (Lactobacillus paracasei) is 2-4:1-3 (body
Product/volume).
The related preservation information being directed to are as follows: China Committee for Culture Collection of Microorganisms's common micro-organisms center；
Address: Yard 1, BeiChen xi Road, Chaoyang District, Beijing City 3, Institute of Microorganism, Academia Sinica, postcode: 100101；Preservation day
Phase: on July 26th, 2018；Deposit number: G1A2 (CGMCC No.16160) is pichia kudriavzevii Pichia
Kudriavzevil, G2B5 (CGMCC No.16161) are lactobacillus paracasei Lactobacillus paracasei.
The present invention also provides a kind of preparation methods of complex microorganism deodorizing microorganism, comprising the following steps:
(1) culture of seed liquor:
The pichia kudriavzevii (Pichia kudriavzevil) G1A2 is inoculated in YPD fluid nutrient medium,
30 DEG C, shaking table 160r/min is cultivated 18 to 30 hours；
The lactobacillus paracasei (Lactobacillus paracasei) G2B5 is inoculated in MRS fluid nutrient medium, 37 DEG C
Static gas wave refrigerator 20 to 28 hours.
(2) optimization culture:
Seed liquor in step (1) is seeded to respectively in 10L fermentor and optimizes culture using Optimal Medium.
Bacterial strain G1A2 condition of culture are as follows: liquid amount 60 ± 5%, inoculum concentration 5 ± 0.05%, cultivation temperature are 31 ± 1 DEG C, revolving speed
250 ± 50 r/min, 2.0 ± 0.1vv of ventilatory capacity.
Bacterial strain G2B5 condition of culture are as follows: liquid amount 80% ± 5%, inoculum concentration 5% ± 0.05%, turn by 35 DEG C ± 1 DEG C of cultivation temperature
50 ± 5r/min of speed.
Incubation time is 18 to 24 hours, and culture to cell concentration reaches 1 × 108A/mL or more order of magnitude obtains
Optimize bacterium solution.
(3) be mixed: the optimization bacterium solution that step (2) is obtained according to G1A2: G2B5=2-4:1-3 of volume ratio ratio
Culture transferring is cultivated into 500L fermentor using mixed culture medium, and condition is mixed are as follows: total inoculum concentration 10 ± 2%, preceding 18 to
24 hours 30 DEG C ± 2 DEG C, 150 ± 50r/min of revolving speed of temperature, ventilatory capacity 1.5 ± 0.5vv, latter 30 hours, 37 ± 1 DEG C, revolving speed 50
± 5 r/min, mixed culture terminate, and obtain complex microorganism deodorizing microorganism.
YPD fluid nutrient medium as described in step (1) is the component being calculated by mass percentage as follows: tryptone
2%, yeast extract 1%, glucose 2%, surplus is water.
The MRS fluid nutrient medium is the component being calculated by mass percentage as follows: tryptone 1%, yeast extract
0.5%, glucose 2%, beef peptone 1%, Tween-80 1%, dipotassium hydrogen phosphate 0.2%, crystallization sodium acetate 0.5%, citric acid three
Ammonium 0.2%, magnesium sulfate 0.02%, manganese sulfate 0.005%, surplus are water, pH6.8.
The Optimal Medium of G1A2 described in step (2) is the component being calculated by mass percentage as follows: peptone 2% ~
4%, yeast extract 1% ~ 3%, glucose 1.8% ~ 2.5%, surplus is water, pH6.8 ± 0.2.
The Optimal Medium of the G2B5 are as follows: tryptone 0.5% ~ 1.5%, yeast extract 0.5% ~ 1%, glucose 2% ~ 4%,
Tween-80 0.5% ~ 1.5%, dipotassium hydrogen phosphate 0.2% ~ 0.4%, crystallization sodium acetate 0.5% ~ 0.6%, magnesium sulfate 0.02% ~ 0.03,
Surplus is water, pH6.0 ± 0.2.
Mixed culture medium described in step (3) are as follows: yeast extract 2% ~ 3%, tryptone 2% ~ 3%, glucose 2% ~ 3%,
Surplus is water, pH6.8 ± 0.2.
Complex microorganism deodorizing microorganism of the present invention is in livestock and poultry farm, garbage transfer station, refuse landfill
Manage the application of rubbish deodorization.
Concrete application method are as follows: 10 ~ 50 parts of tap water are added in a complex microorganism deodorizing microorganism, after mixing directly
It is sprayed on the raw material of required deodorization.
Compared with the prior art, the invention has the beneficial effects that:
Complex microorganism deodorizing microorganism of the invention, using mixed fermentation with various bacterium, by the synergistic effect between different strains, micro- life
Object can make full use of organic matter contained in processing place to be colonized, and inhibit to produce the life of stench microorganism as dominant microflora
Long breeding, fundamentally controls stench source.
The contained bacterial strain of complex microorganism deodorizing microorganism of the invention is highly-safe, is to exempt to do the prebiotic of toxicological test
Bacterium, it is all safer no matter for user or applying environment.
Complex microorganism deodorizing microorganism of the invention passed through to Zymolysis Equipment, fermentation raw material, fermentation condition and fermentation generation
Thank production concentration control and stringent screening, obtained microbial inoculum stable product quality.
Specific embodiment
Embodiments of the present invention are illustrated by particular specific embodiment below.Embodiment is convenient for better understanding this
Invention, but not limitation of the present invention.
One, the preparation method of complex microorganism deodorizing microorganism
Embodiment 1
(1) culture of seed liquor
Pichia kudriavzevii (Pichia kudriavzevil) G1A2 is inoculated in YPD fluid nutrient medium, 30
DEG C, shaking table 160r/min is cultivated 20 hours, obtains G1A2 seed liquor；
Lactobacillus paracasei (Lactobacillus paracasei) G2B5 is inoculated in MRS fluid nutrient medium, 37 DEG C static
Culture 28 hours, obtains G2B5 seed liquor.
(2) optimization culture
Seed liquor in step (1) is seeded to respectively in 10L fermentor and utilizes G1A2 Optimal Medium and G2B5 optimization culture
Base optimizes culture respectively.
The Optimal Medium of G1A2 are as follows: peptone 2%, yeast extract 1%, glucose 2%, surplus are water, pH6.8.
Bacterial strain G1A2 condition of culture are as follows: liquid amount 60%, inoculum concentration 5%, cultivation temperature are 30 DEG C, revolving speed 300r/min, are led to
Tolerance 2.0vv, incubation time are 20 hours, and culture to cell concentration reaches 1 × 108A/mL or more obtains G1A2 optimization bacterium
Liquid.
The Optimal Medium of G2B5 are as follows: tryptone 1%, yeast extract 0.5%, glucose 2%, Tween-80 1%, phosphoric acid hydrogen
Dipotassium 0.2% crystallizes sodium acetate 0.5%, and magnesium sulfate 0.02%, surplus is water, pH6.0.
Bacterial strain G2B5 condition of culture are as follows: liquid amount 80%, inoculum concentration 5%, 35 DEG C of cultivation temperature, revolving speed 45r/min, when culture
Between be 20 hours, culture reaches 1 × 10 to cell concentration8A/mL or more obtains G2B5 optimization bacterium solution.
(3) it is mixed
The G1A2 optimization bacterium solution and G2B5 optimization bacterium solution that step (2) is obtained are moved according to the ratio of G1A2: G2B5=2:3 of volume ratio
Kind is cultivated into 500L fermentor using mixed culture medium, the culture medium of mixed culture are as follows: yeast extract 2%, tryptose
Peptone 2%, glucose 2%, surplus are water, pH6.8.Mixed culture condition are as follows: total inoculum concentration 12%, first 28 DEG C of 20 hours temperature, revolving speed
150r/min, ventilatory capacity 1.5vv, latter 30 hours, 38 DEG C, 55 r/min of revolving speed.Mixed culture terminates, and obtains complex microorganism
Deodorizing microorganism.
Two, the application of complex microorganism deodorizing microorganism
Embodiment 2
The sealing container of 10 identical 100L is taken, is put into the house refuse that 10Kg is derived from treatment of urban garbage station thereto respectively
Sample, wherein 5 are experimental group, 5 are control group.10mL complex microorganism deodorizing microorganism is taken daily, and 490mL tap water is added
It is uniformly sprayed with atomizer on experimental group sample afterwards, makees blank control to spray the tap water that deodorant is not added of same volume,
Continuous sprinkling measured ammonia concentration and concentration of hydrogen sulfide in sample after 5 days.Measurement result is shown in Table 1:
1 complex microorganism deodorizing microorganism of table (50 times of dilution) is to the deodorizing effect of rubbish
| Group | Blank control | Complex microorganism deodorization bacterium | Removal rate (%) |
| Ammonia concentration (mg/m3) | 103.5±5.0 | 34.7±3.2 | 66.5 |
| Concentration of hydrogen sulfide (mg/m3) | 68.3±3.0 | 43.3±2.0 | 36.6 |
As shown in Table 1, when carrying out deodorization to rubbish with product of the present invention, compared with the control group, by complex microorganism deodorizing microorganism
Except ammonia ability reaches 66.5% when diluting 50 times, vulcanisation hydrogen removal rate is 36.6%.
Embodiment 3
The sealing container of 10 identical 100L is taken, is put into the house refuse that 10Kg is derived from treatment of urban garbage station thereto respectively
Sample, wherein 5 are experimental group, 5 position control groups.50mL complex microorganism deodorizing microorganism is taken daily, and 450mL tap water is added
It is uniformly sprayed with atomizer on experimental group sample afterwards, makees blank control to spray the tap water that deodorant is not added of same volume,
Continuous sprinkling measured ammonia concentration and concentration of hydrogen sulfide in sample after 5 days.Measurement result is shown in Table 2:
2 complex microorganism deodorizing microorganism of table (10 times of dilution) is to the deodorizing effect of rubbish
| Group | Blank control | Complex microorganism deodorization bacterium | Removal rate (%) |
| Ammonia concentration (mg/m3) | 103.5±5.0 | 15.3±0.8 | 85.2 |
| Concentration of hydrogen sulfide (mg/m3) | 68.3±3.0 | 14.6±0.2 | 78.6 |
As shown in Table 2, complex microorganism deodorizing microorganism is diluted 10 times in use, except ammonia ability and vulcanisation Hydrogen Energy power reach respectively
To 85.2% and 78.6%, deodorizing effect is obvious.Embodiment 4
In the large-scale poultry place of one scale of level in Anshan city, liaoning province, Chicken Manure Compost place is chosen, wherein 100 ㎡ are as experiment
Group, 100 ㎡ are as a control group.100mL complex microorganism deodorizing microorganism is taken daily, is thought after 900mL tap water is added with atomizer
Experimental group sample uniformly sprays, and is sealed with plastic film.Make blank control to spray the tap water that deodorant is not added of same volume,
Continuous sprinkling measured ammonia concentration and concentration of hydrogen sulfide in test group and control group after 3 days.Measurement result is shown in Table 3:
Deodorizing effect of the 3 complex microorganism deodorizing microorganism of table to fowl and animal excrement
| Group | Blank control | Complex microorganism deodorization bacterium | Removal rate (%) |
| Ammonia concentration (mg/m3) | 149.8±5.0 | 25.3±0.8 | 83.1 |
| Concentration of hydrogen sulfide (mg/m3) | 88.1±3.0 | 24.6±0.2 | 72.1 |
Table 3 is the results show that dilute 10 times in use, continuously i.e. it can be seen that obvious after sprinkling 3 days for complex microorganism deodorizing microorganism
Deodorizing effect dominant microflora in product can be made to colonize and more preferably generate more longlasting deodorization and imitate if lengthening product service life
Fruit.
Claims (10)
1. a kind of complex microorganism deodorizing microorganism, which is characterized in that wherein comprising G1A2 and two kinds of effective bacterium of G2B5, the G1A2
For pichia kudriavzevii (Pichia kudriavzevil), deposit number is CGMCC No.16160；The G2B5
For lactobacillus paracasei (Lactobacillus paracasei), deposit number is CGMCC No.16161.
2. a kind of complex microorganism deodorizing microorganism as described in claim 1, which is characterized in that the library Delhi A Ziweibi
The bacterium solution volume of red yeast (Pichia kudriavzevil) and lactobacillus paracasei (Lactobacillus paracasei)
Ratio is 2-4:1-3.
3. a kind of preparation method of complex microorganism deodorizing microorganism as described in claim 1, which is characterized in that including walking as follows
Suddenly；
(1) culture of seed liquor
The pichia kudriavzevii (Pichia kudriavzevil) G1A2 is inoculated in YPD fluid nutrient medium,
30 DEG C, shaking table 160r/min is cultivated 18 to 30 hours, obtains G1A2 seed liquor；
The lactobacillus paracasei (Lactobacillus paracasei) G2B5 is inoculated in MRS fluid nutrient medium, 37 DEG C
Static gas wave refrigerator 20 to 28 hours, obtain G2B5 seed liquor；
(2) optimization culture
Two kinds of seed liquors in step (1) are seeded to respectively in 10L fermentor and are optimized using G1A2 Optimal Medium and G2B5
Culture medium optimizes culture respectively:
Bacterial strain G1A2 condition of culture are as follows: liquid amount 60 ± 5%, inoculum concentration 5 ± 0.05%, cultivation temperature are 31 ± 1 DEG C, revolving speed
250 ± 50r/min, 2.0 ± 0.1vv of ventilatory capacity obtain G1A2 optimization bacterium solution；
Bacterial strain G2B5 condition of culture are as follows: liquid amount 80% ± 5%, inoculum concentration 5% ± 0.05%, turn by 35 DEG C ± 1 DEG C of cultivation temperature
50 ± 5r/min of speed obtains G2B5 optimization bacterium solution；
Incubation time is 18 to 24 hours, and culture to cell concentration reaches 1 × 108A/mL or more order of magnitude obtains optimization bacterium
Liquid；
(3) it is mixed
After G1A2 optimization bacterium solution and G2B5 optimization bacterium solution that step (2) obtains are combined, culture transferring is sharp into 500L fermentor
It is cultivated with mixed culture medium, is mixed condition are as follows: total inoculum concentration 10 ± 2%, 30 DEG C ± 2 of preceding 18 to 24 hours temperature
DEG C, 150 ± 50r/min of revolving speed, 1.5 ± 0.5vv of ventilatory capacity latter 30 hours, 37 ± 1 DEG C, 50 ± 5r/min of revolving speed, are mixed
Terminate, obtains complex microorganism deodorizing microorganism.
4. a kind of preparation method of complex microorganism deodorizing microorganism according to claim 3, which is characterized in that the step
(3) G1A2 optimizes bacterium solution in and G2B5 optimization bacterium solution is combined according to the ratio of volume ratio G1A2:G2B5=2-4:1-3.
5. a kind of preparation method of complex microorganism deodorizing microorganism according to claim 3, which is characterized in that the YPD
Fluid nutrient medium is the component being calculated by mass percentage as follows: tryptone 2%, yeast extract 1%, and glucose 2% is remaining
Amount is water.
6. a kind of preparation method of complex microorganism deodorizing microorganism according to claim 3, which is characterized in that the G1A2
Optimal Medium is the component being calculated by mass percentage as follows: peptone 2%~4%, yeast extract 1%~3%, grape
Sugar 18%~25%, surplus is water, pH6.8 ± 0.2.
7. a kind of preparation method of complex microorganism deodorizing microorganism according to claim 3, which is characterized in that the G2B5
Optimal Medium are as follows: tryptone 0.5%~1.5%, yeast extract 0.5%~1%, glucose 2%~4%, tween-
800.5%~1.5%, dipotassium hydrogen phosphate 0.2%~0.4%, crystallization sodium acetate 0.5%~0.6%, magnesium sulfate 0.02%~
0.03, surplus is water, pH6.0 ± 0.2.
8. a kind of preparation method of complex microorganism deodorizing microorganism according to claim 3, which is characterized in that step (3)
Described in mixed culture medium are as follows: yeast extract 2%~3%, tryptone 2%~3%, glucose 2%~3%, surplus are
Water, pH6.8 ± 0.2.
9. a kind of application of the complex microorganism deodorizing microorganism according to claim 1 in the deodorizing process of garbage disposal.
10. the application of complex microorganism deodorizing microorganism according to claim 8, concrete application method are as follows: a compound micro-
10~50 parts of tap water are added in biological deodorizing microbial inoculum, are directly sprayed on the raw material of required deodorization after mixing.
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| CN113025507A (en) * | 2021-03-01 | 2021-06-25 | 河北省农林科学院遗传生理研究所（河北省农林科学院农产品质量安全研究中心） | Compound microbial agent, preparation thereof and application thereof in rapid deodorization and purification of high-concentration wastewater in livestock and poultry farms |
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