CN115025136A - Foot bacteriostatic beriberi-removing composition and preparation method thereof - Google Patents
Foot bacteriostatic beriberi-removing composition and preparation method thereof Download PDFInfo
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- CN115025136A CN115025136A CN202210724465.6A CN202210724465A CN115025136A CN 115025136 A CN115025136 A CN 115025136A CN 202210724465 A CN202210724465 A CN 202210724465A CN 115025136 A CN115025136 A CN 115025136A
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- 238000002360 preparation method Methods 0.000 title claims abstract description 16
- 206010047601 Vitamin B1 deficiency Diseases 0.000 title claims abstract description 12
- 208000002894 beriberi Diseases 0.000 title claims abstract description 12
- 239000000203 mixture Substances 0.000 title claims abstract description 12
- 238000000855 fermentation Methods 0.000 claims abstract description 28
- 230000004151 fermentation Effects 0.000 claims abstract description 28
- HDTRYLNUVZCQOY-UHFFFAOYSA-N α-D-glucopyranosyl-α-D-glucopyranoside Natural products OC1C(O)C(O)C(CO)OC1OC1C(O)C(O)C(O)C(CO)O1 HDTRYLNUVZCQOY-UHFFFAOYSA-N 0.000 claims abstract description 8
- WHUUTDBJXJRKMK-UHFFFAOYSA-N Glutamic acid Natural products OC(=O)C(N)CCC(O)=O WHUUTDBJXJRKMK-UHFFFAOYSA-N 0.000 claims abstract description 8
- CKLJMWTZIZZHCS-REOHCLBHSA-N L-aspartic acid Chemical compound OC(=O)[C@@H](N)CC(O)=O CKLJMWTZIZZHCS-REOHCLBHSA-N 0.000 claims abstract description 8
- AYFVYJQAPQTCCC-UHFFFAOYSA-N Threonine Natural products CC(O)C(N)C(O)=O AYFVYJQAPQTCCC-UHFFFAOYSA-N 0.000 claims abstract description 8
- 239000004473 Threonine Substances 0.000 claims abstract description 8
- HDTRYLNUVZCQOY-WSWWMNSNSA-N Trehalose Natural products O[C@@H]1[C@@H](O)[C@@H](O)[C@@H](CO)O[C@@H]1O[C@@H]1[C@H](O)[C@@H](O)[C@@H](O)[C@@H](CO)O1 HDTRYLNUVZCQOY-WSWWMNSNSA-N 0.000 claims abstract description 8
- HDTRYLNUVZCQOY-LIZSDCNHSA-N alpha,alpha-trehalose Chemical compound O[C@@H]1[C@@H](O)[C@H](O)[C@@H](CO)O[C@@H]1O[C@@H]1[C@H](O)[C@@H](O)[C@H](O)[C@@H](CO)O1 HDTRYLNUVZCQOY-LIZSDCNHSA-N 0.000 claims abstract description 8
- 235000003704 aspartic acid Nutrition 0.000 claims abstract description 8
- OQFSQFPPLPISGP-UHFFFAOYSA-N beta-carboxyaspartic acid Natural products OC(=O)C(N)C(C(O)=O)C(O)=O OQFSQFPPLPISGP-UHFFFAOYSA-N 0.000 claims abstract description 8
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- 235000013922 glutamic acid Nutrition 0.000 claims abstract description 8
- 239000004220 glutamic acid Substances 0.000 claims abstract description 8
- 235000008521 threonine Nutrition 0.000 claims abstract description 8
- UHVMMEOXYDMDKI-JKYCWFKZSA-L zinc;1-(5-cyanopyridin-2-yl)-3-[(1s,2s)-2-(6-fluoro-2-hydroxy-3-propanoylphenyl)cyclopropyl]urea;diacetate Chemical compound [Zn+2].CC([O-])=O.CC([O-])=O.CCC(=O)C1=CC=C(F)C([C@H]2[C@H](C2)NC(=O)NC=2N=CC(=CC=2)C#N)=C1O UHVMMEOXYDMDKI-JKYCWFKZSA-L 0.000 claims abstract description 8
- 108010082495 Dietary Plant Proteins Proteins 0.000 claims abstract description 5
- WHUUTDBJXJRKMK-VKHMYHEASA-N L-glutamic acid Chemical compound OC(=O)[C@@H](N)CCC(O)=O WHUUTDBJXJRKMK-VKHMYHEASA-N 0.000 claims abstract description 3
- AYFVYJQAPQTCCC-GBXIJSLDSA-N L-threonine Chemical compound C[C@@H](O)[C@H](N)C(O)=O AYFVYJQAPQTCCC-GBXIJSLDSA-N 0.000 claims abstract description 3
- 239000007788 liquid Substances 0.000 claims description 36
- 239000001963 growth medium Substances 0.000 claims description 18
- 238000012258 culturing Methods 0.000 claims description 12
- 238000009630 liquid culture Methods 0.000 claims description 12
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- 229940041514 candida albicans extract Drugs 0.000 claims description 9
- 239000008103 glucose Substances 0.000 claims description 9
- 239000006228 supernatant Substances 0.000 claims description 9
- 239000012137 tryptone Substances 0.000 claims description 9
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 9
- 239000012138 yeast extract Substances 0.000 claims description 9
- 241000186605 Lactobacillus paracasei Species 0.000 claims description 8
- 150000001875 compounds Chemical class 0.000 claims description 7
- 239000000843 powder Substances 0.000 claims description 7
- CSNNHWWHGAXBCP-UHFFFAOYSA-L Magnesium sulfate Chemical compound [Mg+2].[O-][S+2]([O-])([O-])[O-] CSNNHWWHGAXBCP-UHFFFAOYSA-L 0.000 claims description 6
- 239000002244 precipitate Substances 0.000 claims description 6
- 238000005303 weighing Methods 0.000 claims description 6
- 241000894006 Bacteria Species 0.000 claims description 5
- 241000235648 Pichia Species 0.000 claims description 5
- 230000000844 anti-bacterial effect Effects 0.000 claims description 5
- 239000000706 filtrate Substances 0.000 claims description 5
- 238000000034 method Methods 0.000 claims description 5
- 239000001888 Peptone Substances 0.000 claims description 3
- 108010080698 Peptones Proteins 0.000 claims description 3
- 108010064851 Plant Proteins Proteins 0.000 claims description 3
- VMHLLURERBWHNL-UHFFFAOYSA-M Sodium acetate Chemical compound [Na+].CC([O-])=O VMHLLURERBWHNL-UHFFFAOYSA-M 0.000 claims description 3
- 235000015278 beef Nutrition 0.000 claims description 3
- 239000013078 crystal Substances 0.000 claims description 3
- ZPWVASYFFYYZEW-UHFFFAOYSA-L dipotassium hydrogen phosphate Chemical compound [K+].[K+].OP([O-])([O-])=O ZPWVASYFFYYZEW-UHFFFAOYSA-L 0.000 claims description 3
- 238000011081 inoculation Methods 0.000 claims description 3
- 229910052943 magnesium sulfate Inorganic materials 0.000 claims description 3
- 235000019341 magnesium sulphate Nutrition 0.000 claims description 3
- 229940099596 manganese sulfate Drugs 0.000 claims description 3
- 235000007079 manganese sulphate Nutrition 0.000 claims description 3
- 239000011702 manganese sulphate Substances 0.000 claims description 3
- SQQMAOCOWKFBNP-UHFFFAOYSA-L manganese(II) sulfate Chemical compound [Mn+2].[O-]S([O-])(=O)=O SQQMAOCOWKFBNP-UHFFFAOYSA-L 0.000 claims description 3
- 239000012528 membrane Substances 0.000 claims description 3
- 238000002156 mixing Methods 0.000 claims description 3
- 235000019319 peptone Nutrition 0.000 claims description 3
- 235000021118 plant-derived protein Nutrition 0.000 claims description 3
- 239000001632 sodium acetate Substances 0.000 claims description 3
- 235000017281 sodium acetate Nutrition 0.000 claims description 3
- 238000003756 stirring Methods 0.000 claims description 3
- 238000000967 suction filtration Methods 0.000 claims description 3
- YWYZEGXAUVWDED-UHFFFAOYSA-N triammonium citrate Chemical compound [NH4+].[NH4+].[NH4+].[O-]C(=O)CC(O)(CC([O-])=O)C([O-])=O YWYZEGXAUVWDED-UHFFFAOYSA-N 0.000 claims description 3
- 239000001393 triammonium citrate Substances 0.000 claims description 3
- 235000011046 triammonium citrate Nutrition 0.000 claims description 3
- 238000012136 culture method Methods 0.000 claims description 2
- 229910000396 dipotassium phosphate Inorganic materials 0.000 claims description 2
- 235000019797 dipotassium phosphate Nutrition 0.000 claims description 2
- LWIHDJKSTIGBAC-UHFFFAOYSA-K potassium phosphate Substances [K+].[K+].[K+].[O-]P([O-])([O-])=O LWIHDJKSTIGBAC-UHFFFAOYSA-K 0.000 claims description 2
- 238000009631 Broth culture Methods 0.000 claims 1
- 241000223229 Trichophyton rubrum Species 0.000 abstract description 14
- 230000000694 effects Effects 0.000 abstract description 12
- 208000002474 Tinea Diseases 0.000 abstract description 4
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- 230000009982 effect on human Effects 0.000 abstract 1
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- FPAFDBFIGPHWGO-UHFFFAOYSA-N dioxosilane;oxomagnesium;hydrate Chemical compound O.[Mg]=O.[Mg]=O.[Mg]=O.O=[Si]=O.O=[Si]=O.O=[Si]=O.O=[Si]=O FPAFDBFIGPHWGO-UHFFFAOYSA-N 0.000 description 2
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- JVTAAEKCZFNVCJ-UHFFFAOYSA-N lactic acid Chemical compound CC(O)C(O)=O JVTAAEKCZFNVCJ-UHFFFAOYSA-N 0.000 description 2
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- YGSDEFSMJLZEOE-UHFFFAOYSA-N salicylic acid Chemical compound OC(=O)C1=CC=CC=C1O YGSDEFSMJLZEOE-UHFFFAOYSA-N 0.000 description 2
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- 238000006467 substitution reaction Methods 0.000 description 2
- 208000002655 Foot Dermatoses Diseases 0.000 description 1
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- OYHQOLUKZRVURQ-HZJYTTRNSA-N Linoleic acid Chemical compound CCCCC\C=C/C\C=C/CCCCCCCC(O)=O OYHQOLUKZRVURQ-HZJYTTRNSA-N 0.000 description 1
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- 210000002510 keratinocyte Anatomy 0.000 description 1
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- FJKROLUGYXJWQN-UHFFFAOYSA-N papa-hydroxy-benzoic acid Natural products OC(=O)C1=CC=C(O)C=C1 FJKROLUGYXJWQN-UHFFFAOYSA-N 0.000 description 1
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- WXMKPNITSTVMEF-UHFFFAOYSA-M sodium benzoate Chemical group [Na+].[O-]C(=O)C1=CC=CC=C1 WXMKPNITSTVMEF-UHFFFAOYSA-M 0.000 description 1
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- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/14—Fungi; Culture media therefor
- C12N1/16—Yeasts; Culture media therefor
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- A—HUMAN NECESSITIES
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- A61K35/741—Probiotics
- A61K35/744—Lactic acid bacteria, e.g. enterococci, pediococci, lactococci, streptococci or leuconostocs
- A61K35/747—Lactobacilli, e.g. L. acidophilus or L. brevis
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Abstract
The invention relates to the technical field of microbial engineering, in particular to a foot bacteriostatic beriberi-removing composition and a preparation method thereof. The composition comprises biological fermentation liquor, vegetable protein, carrageenan, trehalose, aspartic acid, threonine and glutamic acid. The content ratio of each component except the biological fermentation liquor is respectively as follows: 0.01-0.1% of vegetable protein, 0.01-0.5% of carrageenan, 0.01-0.2% of trehalose, 0.01-0.2% of aspartic acid, 0.01-0.2% of threonine and 0.01-0.2% of glutamic acid. The trichophyton rubrum has obvious inhibition effect on human pathogenic fungi, has low raw material cost, obvious effect and mild effect, and avoids the problem of mild treatment of dermatophytosis, thereby having good application value.
Description
Technical Field
The invention relates to the technical field of microbial engineering, in particular to a foot bacteriostatic beriberi-removing composition and a preparation method thereof.
Background
At present, the problem of beriberi is common in society, and the beriberi usually shows three characteristics of dryness, itching and odor. The foot deodorant is characterized in that the cuticle in the foot skin is extremely rich and contains a large amount of keratin, and simultaneously contains a large amount of sweat glands, but the distribution of the sebaceous glands is far lower than that of other skins, the foot ventilation and heat dissipation environment is poor, a large amount of sweat secreted by the sweat glands cannot be dissipated timely, the sebaceous glands are fewer and cannot generate enough fatty acid to inhibit the growth of bacteria and fungi, and in addition, the foot deodorant, namely foot odor, can be generated by the fact that the foot cuticle protein is large in amount and the foot bacteria and fungi are easily bred excessively, the bacteria breed and decompose the cuticle protein in large amount, and then urea and lactic acid in sweat are mixed. The reason for the dry foot is mainly that the keratinocyte is too thick, the moisture is insufficient, and the foot itching is mainly that the activity of the ringworm stimulates the nerves of the foot. At present, most of common pathogenic microorganisms of feet are fungi ringworm, common bacterial colonies are trichophyton rubrum, trichophyton filiformis and the like, and partial bacteria are staphylococcus aureus and the like.
At the present stage, most of the products sold in the market for treating foot problems are mainly chemical bacteriostatic substances and are disinfectant with a word eliminating number, and the main principle of the products is as follows: first, the cell walls of foot colonies are disrupted, resulting in colonies being non-viable, but the irritation of such bactericidal products is usually greater. Secondly, the sweat that dry powder class material (like the talcum powder) can effectual absorption foot secretion through in time neutralizing the absorption with the sweat, reduces the wet and slippery sense and the slimy sense of foot, changes local environment, leads to the bacterial colony from the source not have moisture unable growth, but this class material is difficult to carry because of using complicacy, and the poor reverberation of lasting effect is not good, and because of material granularity such as talcum powder is less, blocks up the pore very easily, leads to the inflammation. Thirdly, the metabolism of skin cells is accelerated, the shedding of stratum corneum cells is accelerated, and then colonies are removed integrally, the raw materials mainly comprise salicylic acid, linoleic acid and the like, but the irritation of acid substances and the damage to the skin are large, so that people feel afraid of mind when using the hair conditioner.
Aiming at the defects of single function, insignificant effect, large side effect and the like of the products sold in the market and the difficulty in meeting the requirement of the current market for improving the foot problems, the project takes the biological fermentation technology as the leading factor, and the foot antibacterial compound liquid is prepared by compound preparation, so that the blank of the products in the market is made up by the characteristics of mild action, safety, effectiveness, multiple targets and the like.
Disclosure of Invention
In order to solve the problems, namely the problems brought forward by the background technology, the invention provides a foot bacteriostatic beriberi-removing composition and a preparation method thereof, and the technical scheme is as follows:
(1) preparation of biological fermentation broth
Culture of seed liquid
Inoculating the Pichia kudriavzevil into a YPD liquid culture medium, and culturing at 30 ℃ and 160rpm of a shaking table for 18-30h to obtain a seed solution A; the YPD liquid culture medium comprises the following components: 1-2% of tryptone, 1-2% of yeast extract powder, 1-2% of glucose and the balance of water;
inoculating the Lactobacillus paracasei (Lactobacillus paracasei) into an MRS liquid culture medium, and statically culturing for 20-28h at 37 ℃ to obtain a seed liquid B; the formula of the MRS liquid culture medium is as follows: 1% of tryptone, 0.5-1% of yeast extract powder, 1-2% of glucose, 1-2% of beef peptone, 1-2% of tween-801-2%, 0.2-1% of dipotassium phosphate, 0.5-1% of sodium acetate crystal, 0.2-1% of triammonium citrate, 0.02-0.1% of magnesium sulfate, 0.005-0.01% of manganese sulfate, the balance of water and pH 6.5-7.0;
② mixed culture
Mixing the seed solution A and the seed solution B obtained in the step I, wherein the volume ratio of the two seed solutions is 2-4:1-3, transferring the two seed solutions into a 500mL conical flask, and continuously culturing by using a mixed culture medium. The mixed culture medium comprises the following components: 2-3% of yeast extract, 2-3% of tryptone, 2-3% of glucose and the balance of water, wherein the pH value is 6.5-7.0.
The mixed culture conditions are as follows: the total inoculation amount of the mixed seed liquid is 5-10%; the culture method comprises the following steps: the temperature is 28-32 ℃ in the first 18-24 hours, and the rotation speed is 100-200 rpm; then continuously culturing for 24 hours at the temperature of 36-38 ℃ and the rotating speed of 45-55rpm, and obtaining the composite fermentation liquor after the mixed culture is finished;
preparation of biological fermentation liquid for work
Centrifuging the composite fermentation liquid obtained in the step two for 15-30min under the conditions of 2-8 ℃ and 5000-plus-one rpm, discarding the precipitate, centrifuging the supernatant for 15-30min under the conditions of 2-8 ℃ and 5000-plus-one 10000rpm, discarding the precipitate, and collecting the supernatant into a transparent silk mouth bottle. Transferring the supernatant of the silk mouth bottle into a filter, performing suction filtration in a sample hole of the filter under the pressure of 0.1-0.5MPa by using a filter membrane with the specification of 0.22 mu m, and catching the filtrate by using a beaker, wherein the filtrate is the biological fermentation liquid for work;
(2) preparation of bacteriostatic composite liquid
Weighing 1L of the working biological fermentation liquid in (1) by using a measuring cylinder, respectively weighing corresponding components according to the formula proportion of 0.01-0.1% of plant protein, 0.01-0.5% of carrageenan, 0.01-0.2% of trehalose, 0.01-0.2% of aspartic acid, 0.01-0.2% of threonine and 0.01-0.2% of glutamic acid, then adding the corresponding components into the working biological fermentation liquid, and stirring and dissolving for 2-4 hours at the temperature of 18-26 ℃ by using a stirrer with the rotating speed of 20-50rpm, thus obtaining the antibacterial complex liquid.
The beneficial technical effects of the invention are as follows: the raw materials adopted have the characteristics of low cost, obvious effect, mild effect, safety, practicability, convenient use and the like, and simultaneously the problems of severe effect and foot injury and skin injury in the traditional medicine treatment of beriberi are avoided, so that the traditional Chinese medicine has better application value.
Drawings
Figure 1 shows a schematic of the product of the invention.
FIG. 2 shows the colony morphology of Trichophyton rubrum in a blank example of the present invention.
FIG. 3 shows the colony morphology of Trichophyton rubrum according to an embodiment of the present invention.
Detailed Description
Preferred embodiments of the present invention are described below with reference to the accompanying drawings. It should be understood by those skilled in the art that these embodiments are only for explaining the technical principle of the present invention, and are not intended to limit the scope of the present invention.
Example one
TABLE 1 Specification, type and origin of the raw materials
The invention provides a foot bacteriostatic compound liquid which is composed of biological fermentation liquid, vegetable protein, carrageenan, trehalose, aspartic acid, threonine and glutamic acid. The method is as follows
(1) Preparation of biological fermentation broth
Culture of seed liquid
Inoculating the Pichia kudriavzevil into a YPD liquid culture medium, and culturing at 30 ℃ and 160rpm of a shaking table for 30 hours to obtain a seed solution A; the YPD liquid culture medium comprises the following components: 2% of tryptone, 1% of yeast extract powder, 2% of glucose and the balance of water.
Inoculating the Lactobacillus paracasei (Lactobacillus paracasei) into an MRS liquid culture medium, and statically culturing for 28 hours at 37 ℃ to obtain a seed solution B; the formula of the MRS liquid culture medium is as follows: tryptone 1%, yeast extract powder 0.5%, glucose 2%, beef peptone 1%, tween-801%, dipotassium hydrogen phosphate 0.2%, sodium acetate crystal 0.5%, triammonium citrate 0.2%, magnesium sulfate 0.02%, manganese sulfate 0.005%, and the balance of water, and the pH value is 6.8.
② mixed culture
Mixing the seed solution A and the seed solution B obtained in the step I, wherein the volume ratio of the two seed solutions is 2-4:1-3, then transplanting the seed solution into a 500mL conical flask, and continuing culturing by using a mixed culture medium. The mixed culture medium comprises the following components: 2% of yeast extract, 2% of tryptone, 2% of glucose and the balance of water, wherein the pH value is 6.8 +/-0.2. The mixed culture conditions are as follows: the total inoculation amount is 10 percent, the temperature is 30 ℃ in the first 18 to 24 hours, and the rotating speed is 150 rpm; and then continuously culturing for 24 hours at the temperature of 37 ℃ and the rotating speed of 50rpm, and finishing the mixed culture to obtain the compound fermentation.
Preparation of biological fermentation liquid for work
Centrifuging the composite fermentation obtained in the second step for 30min at 4 ℃ and 3000rpm, discarding the precipitate, centrifuging the supernatant for 30min at 4 ℃ and 8000rpm, discarding the precipitate, and collecting the supernatant into a transparent silk-mouth bottle. Transferring the supernatant of the silk-mouth bottle into a filter, performing suction filtration at 0.1MPa by using a filter membrane with the specification of 0.22 mu m in a sample hole of the filter, and catching the filtrate by using a beaker, thereby obtaining the biological fermentation liquid for work.
(2) Preparation of bacteriostatic composite liquid
Weighing 1L of the working biological fermentation liquid in (1) by using a measuring cylinder, respectively weighing corresponding components according to the formula proportion of 0.05% of plant protein, 0.2% of carrageenan, 0.1% of trehalose, 0.1% of aspartic acid, 0.1% of threonine and 0.1% of glutamic acid, then adding the components into the working biological fermentation liquid, and stirring and dissolving for 4 hours at the temperature of 25 ℃ by using a stirrer with the rotating speed of 30rpm, thus obtaining the antibacterial compound liquid.
(3) Bacteriostasis experiment of bacteriostasis composite liquid
Principle of experiment
Tinea pedis (commonly known as dermatophytosis) is a foot dermatosis caused by pathogenic fungi (mainly trichophyton rubrum) and has infectivity. Tinea pedis is widely prevalent throughout the world, and is more prevalent in tropical and subtropical regions. In China, the incidence of tinea pedis is also quite high. There is no sebaceous gland between foot and toe of human, so there is no fatty acid for inhibiting skin filamentous fungi, and physiological defense function is poor, while skin sweat glands of these parts are abundant, sweating is more, and air circulation is poor, local moisture and warmth are good for growth of filamentous fungi. In addition, the skin stratum corneum of the sole part is thick, and keratin in the stratum corneum is an ideal nutrient for fungus breeding and is beneficial to the growth of fungi.
Trichophyton rubrum is a kind of Trichophyton, is a human-derived dermatophytosis, and causes common skin superficial mycosis, such as tinea manuum, tinea pedis, tinea capitis, and the like. The project is to evaluate the efficacy of the foot bacteriostatic compound liquid by verifying the inhibition result of trichophyton rubrum according to the generation process of the infected trichophyton rubrum on the skin of feet of a human body. In the inhibition test of the trichophyton rubrum, the inhibition effect of the foot bacteriostatic composite liquid on the trichophyton rubrum is judged by measuring the colony size of the trichophyton rubrum. In order to eliminate the influence of other preservatives on the efficacy test effect, the test also sets a blank control, the preservative is sodium benzoate adopted in the blank group, and the used concentration is 1 percent of the commonly used maximum addition concentration.
② evaluation standard of bacteriostatic efficacy
TABLE 5-1 evaluation criteria for bacteriostatic efficacy
(iii) test results
TABLE 5-2 efficacy test results for samples inhibiting Trichophyton rubrum
| Serial number | Sample name | Efficacy of | Diameter of colony growth |
| 1 | Examples | Is effective | 12±1mm |
| 2 | Blank example | Invalidation | 30±2mm |
The test result shows that the blank case has no bacteriostatic effect on the trichophyton rubrum, and the foot bacteriostatic composite liquid has good effect of inhibiting the trichophyton rubrum and shows good bacteriostatic effect.
While the invention has been described with reference to a preferred embodiment, various changes may be made and equivalents may be substituted for elements thereof without departing from the scope of the invention, and particularly, features shown in the various embodiments may be combined in any suitable manner without departing from the scope of the invention. It is intended that the invention not be limited to the particular embodiments disclosed, but that the invention will include all embodiments falling within the scope of the appended claims.
In the description of the present invention, the terms "center", "upper", "lower", "left", "right", "vertical", "horizontal", "inner", "outer", and the like, which indicate a directional or positional relationship, are based on the directional or positional relationship as shown in the drawings, which are merely for convenience of description, and do not indicate or imply that the device or element must have a particular orientation, be constructed in a particular orientation, and be operated, and thus, should not be construed as limiting the present invention. Furthermore, the terms "first," "second," and "third" are used for descriptive purposes only and are not to be construed as indicating or implying relative importance.
Furthermore, it should be noted that, in the description of the present invention, unless otherwise explicitly specified or limited, the terms "mounted," "connected," and "connected" are to be construed broadly, and may be, for example, fixedly connected, detachably connected, or integrally connected; can be mechanically or electrically connected; they may be connected directly or indirectly through intervening media, or they may be interconnected between two elements. The specific meanings of the above terms in the present invention can be understood by those skilled in the art according to specific situations.
The terms "comprises," "comprising," or any other similar term are intended to cover a non-exclusive inclusion, such that a process, article, or apparatus that comprises a list of elements does not include only those elements but may include other elements not expressly listed or inherent to such process, article, or apparatus. So far, the technical solutions of the present invention have been described in connection with the preferred embodiments shown in the drawings, but it is easily understood by those skilled in the art that the scope of the present invention is obviously not limited to these specific embodiments. Equivalent changes or substitutions of related technical features can be made by those skilled in the art without departing from the principle of the invention, and the technical scheme after the changes or substitutions can fall into the protection scope of the invention.
Claims (4)
1. The foot bacteriostatic beriberi-removing composition is characterized by comprising the following components in parts by weight: the composition is composed of biological fermentation liquor, vegetable protein, carrageenan, trehalose, aspartic acid, threonine and glutamic acid.
2. The bacteriostatic beriberi-removing composition for feet according to claim 1, which is characterized in that: the biological fermentation liquid is prepared by fermenting two bacteria of Pichia kudriavzevil (Pichia kudriavzevil) and Lactobacillus paracasei (Lactobacillus paracasei).
3. The foot bacteriostatic beriberi-removing composition according to claim 1, which is characterized in that: in the composition, the content ratios of the components except the biological fermentation liquid are respectively as follows: 0.01-0.1% of vegetable protein, 0.01-0.5% of carrageenan, 0.01-0.2% of trehalose, 0.01-0.2% of aspartic acid, 0.01-0.2% of threonine and 0.01-0.2% of glutamic acid.
4. The preparation method of the foot bacteriostatic beriberi-removing composition according to claim 1, which is characterized by comprising the following steps: the method comprises the following steps:
(1) preparation of biological fermentation broth
Culture of seed liquid
Inoculating the Pichia kudriavzevil into a YPD liquid culture medium, and culturing at 30 ℃ and 160rpm of a shaking table for 18-30h to obtain a seed solution A; the YPD liquid culture medium comprises the following components: 1-2% of tryptone, 1-2% of yeast extract powder, 1-2% of glucose and the balance of water;
inoculating the Lactobacillus paracasei (Lactobacillus paracasei) into an MRS liquid culture medium, and statically culturing for 20-28h at 37 ℃ to obtain a seed liquid B; the formula of the MRS liquid culture medium is as follows: 1% of tryptone, 0.5-1% of yeast extract powder, 1-2% of glucose, 1-2% of beef peptone, 1-2% of tween-801-2%, 0.2-1% of dipotassium phosphate, 0.5-1% of sodium acetate crystal, 0.2-1% of triammonium citrate, 0.02-0.1% of magnesium sulfate, 0.005-0.01% of manganese sulfate, the balance of water and pH 6.5-7.0;
② mixed culture
Mixing the seed solution A and the seed solution B obtained in the step I, wherein the volume ratio of the two seed solutions is 2-4:1-3, transplanting the seed solution into a 500mL conical flask, and continuously culturing by using a mixed culture medium. The mixed culture medium comprises the following components: 2-3% of yeast extract, 2-3% of tryptone, 2-3% of glucose and the balance of water, wherein the pH value is 6.5-7.0.
The mixed culture conditions are as follows: the total inoculation amount of the mixed seed liquid is 5-10%; the culture method comprises the following steps: the temperature is 28-32 ℃ in the first 18-24 hours, and the rotation speed is 100-200 rpm; then continuously culturing for 24 hours at the temperature of 36-38 ℃ and the rotating speed of 45-55rpm, and obtaining the composite fermentation liquor after the mixed culture is finished;
preparation of biological fermentation liquid for work
Centrifuging the composite fermentation liquid obtained in the step two for 15-30min under the conditions of 2-8 ℃ and 5000-plus-one rpm, discarding the precipitate, centrifuging the supernatant for 15-30min under the conditions of 2-8 ℃ and 5000-plus-one 10000rpm, discarding the precipitate, and collecting the supernatant into a transparent silk mouth bottle. Transferring the supernatant of the silk mouth bottle into a filter, performing suction filtration in a sample hole of the filter under the pressure of 0.1-0.5MPa by using a filter membrane with the specification of 0.22 mu m, and catching the filtrate by using a beaker, wherein the filtrate is the biological fermentation liquid for work;
(2) preparation of bacteriostatic composite liquid
Weighing 1L of the working biological fermentation liquid in the step (1) by using a measuring cylinder, respectively weighing corresponding components according to the formula proportion of 0.01-0.1% of plant protein, 0.01-0.5% of carrageenan, 0.01-0.2% of trehalose, 0.01-0.2% of aspartic acid, 0.01-0.2% of threonine and 0.01-0.2% of glutamic acid, then adding the corresponding components into the working biological fermentation liquid, and stirring and dissolving the components for 2-4 hours at the temperature of 18-26 ℃ by using a stirrer with the rotating speed of 20-50rpm, thus obtaining the antibacterial compound liquid.
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Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101716337A (en) * | 2009-12-10 | 2010-06-02 | 马照芳 | Liquid medicine for treating dermatophytosis and tinea pedis |
| CN110358699A (en) * | 2019-05-10 | 2019-10-22 | 基因赛奥(大连)生物科技发展有限公司 | A kind of complex microorganism deodorizing microorganism and the preparation method and application thereof |
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| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101716337A (en) * | 2009-12-10 | 2010-06-02 | 马照芳 | Liquid medicine for treating dermatophytosis and tinea pedis |
| CN110358699A (en) * | 2019-05-10 | 2019-10-22 | 基因赛奥(大连)生物科技发展有限公司 | A kind of complex microorganism deodorizing microorganism and the preparation method and application thereof |
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