CN113288830A - A method for preparing cosmetic raw material composition containing filtrate of fermentation product of Chaba and truffle, and its application - Google Patents
A method for preparing cosmetic raw material composition containing filtrate of fermentation product of Chaba and truffle, and its application Download PDFInfo
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Abstract
The invention provides a cosmetic raw material composition containing a filtrate of a truffle and inonotus obliquus fermentation product, which comprises the following components: filtrate containing fermentation product of Chaba Tricholoma matsutake, filtrate containing mixed fermentation product of Bacillus bifidus of Chaba Tricholoma matsutake, 1, 2-hexanediol, and p-hydroxyacetophenone; and the content of the 1, 2-hexanediol is 0.5-2%, the content of the p-hydroxyacetophenone is 0.1-1%, the filtrate of the fermentation product containing the chaga yeast is 80-90%, and the filtrate of the mixed fermentation product containing the chaga lactobacillus and bifidobacterium is remained. The invention introduces the truffle and the inonotus obliquus fermentation product into the field of cosmetics, enlarges the application range of the truffle and the inonotus obliquus, obviously ensures that the main substances are prepared by natural fermentation, has the characteristics of safety and environmental protection, has richer active substances, is rich in substances such as polypeptide, amino acid, organic acid, vitamin and the like, can improve skin quality, promotes metabolism, further relieves the skin problem caused by ecological balance of the skin surface, and achieves the effect of skin care.
Description
Technical Field
The invention relates to the technical field of cosmetics, in particular to a preparation method and application of a cosmetic raw material composition containing a filtrate of a truffle and inonotus obliquus fermentation product.
Background
Fermentation refers to the process by which a person produces the microbial cells themselves, or direct or secondary metabolites, by virtue of the life activities of the microorganism under aerobic or anaerobic conditions. Generally, fermentation refers to a process of decomposing organic substances by organisms. Fermentation is a biochemical reaction that humans have come into contact with earlier and is now widely used in the food industry, the biological and chemical industries.
Yeast is a microorganism variety which is first utilized by human beings for commercial development, is also the most important one, is also a main strain for alcohol fermentation, and can obtain yeast fermentation filtrate extracts of various varieties by screening different yeast varieties and different nutrition and stress conditions. The yeast fermentation product filtrate contains a large amount of natural active ingredient substances such as micromolecular amino acid, polypeptide, nucleotide, vitamin and the like, and is a good comprehensive skin conditioner. The yeast filtrate is in weak acidity, and has obvious inhibition effect on harmful bacteria on the skin surface. Because the skin-care product is more suitable for the survival of harmful bacteria of the skin in an alkaline environment, the skin acid environment is kept to a certain extent, the infection can be effectively resisted, the barrier effect of the stratum corneum is fully exerted, and the healthy skin micro-ecology is kept. The yeast fermentation product filtrate can balance grease, moisten white and lighten spots, resist oxidation and repair skin; the Natural Moisturizing Factor (NMF) is rich in moisture for promoting the full moisture of the horny layer, and is beneficial to the absorption and transformation of nutrient substances.
The general perception of probiotics is mostly retained by its benefits to the gut. In fact, it also has its own unique advantages for skin. When acting on the skin surface, the composition can affect the microbial flora of the skin, so that the skin is kept in a balanced state. Thereby further relieving the skin problem caused by the ecological balance of the skin surface and achieving the effect of skin care. Lactic acid bacteria and bifidobacteria are the best known probiotics.
Truffle is a kind of large lump fungus of ascomycete, also called truffle. Researches show that the black pine dew contains abundant polysaccharides, proteins, amino acids and polyunsaturated fatty acids, and also contains abundant minerals and sterols. The existing research shows that truffle has strong biological activity in the aspects of antibiosis, bacteriostasis and antioxidation. Meanwhile, ceramide is one of effective active ingredients of truffle. Ceramide is formed by dehydration condensation of fatty acid and sphingosine through amido bond. Ceramide is a water-soluble lipid substance, has a similar keratin structure to the skin, promotes the regeneration of keratin, and is used in large quantities for the production of cosmetics.
Inonotus obliquus is a medicinal fungus growing on white birch. The Inonotus obliquus has antibacterial, antioxidant and protecting effects, contains abundant vitamins and minerals, and is helpful for preventing cell aging and promoting metabolism. In addition, the content of superoxide dismutase of the inonotus obliquus is the first in the mushroom field, and the inonotus obliquus has strong anti-inflammatory and antioxidant components and can repair cell damage caused by long-term skin inflammation. Meanwhile, researches show that the inonotus obliquus contains active substances such as polysaccharides, triterpenes, anthocyanins and the like, and has a good effect of removing free radicals in organisms.
The honey contains a large amount of vitamins, amino acids and various active substances, and has strong permeability, water retention property and hygroscopicity, so that the honey has more health care functions, such as blood circulation promotion, endocrine imbalance treatment, body metabolic circulation promotion and the like. Therefore, the cosmetic composition is widely applied to cosmetics. The honey can effectively sterilize and inhibit the growth of bacteria, and does not harm the skin surface. Because of this, cosmetics containing honey are highly appreciated because they can make the skin surface smoother and have no side effects.
With the continuous development and growth of the domestic cosmetic market, consumers pursue the face appearance to be continuously upgraded, and the public also puts forward new expectations and requirements for cosmetics and cosmetic raw materials. The cosmetic is effective, natural in components and low in irritation, and does not simply pursue the effect of cosmetics and take effect for a long time. And the cosmetics from fermentation sources have obvious advantages in the aspects, so that the market prefers the cosmetics. The skin is used as the first barrier of the human body against external invasion and is tested all the time. In addition, the skin is seriously asked by factors such as fast-paced life and air pollution in the present stage. Skin problems continue to emerge in these cases as well. The public is eagerly looking for products capable of caring skin and improving skin quality, and only the traditional chemical synthetic products cannot motivate people. Meanwhile, good skin also reflects the essence, spirit and state of the human body.
Disclosure of Invention
In order to overcome the increasingly serious skin problems in the prior art and the increasingly high requirements of human beings on cosmetic raw materials, the invention provides a preparation method and application of a cosmetic raw material composition containing a filtrate of a fermentation product of the inonotus obliquus, wherein the cosmetic raw material composition containing the filtrate of the fermentation product of the inonotus obliquus is a filtrate of the fermentation product of the inonotus obliquus, a filtrate of a mixed fermentation product of bifidobacterium containing the lactobacillus of the inonotus obliquus is 1, 2-hexanediol and p-hydroxyacetophenone, the content of the 1, 2-hexanediol is 0.5-2%, the content of the p-hydroxyacetophenone is 0.1-1%, the filtrate of the fermentation product of the bifidobacterium containing the inonotus is 80-90%, and the rest is a filtrate of the mixed fermentation product of the bifidobacterium containing the inonotus obliquus.
By adopting the technical scheme, the 1, 2-hexanediol is added into the compounded fermentation filtrate, the 1, 2-hexanediol is an excellent solvent and also has the solubilizing capability, stable product performance can be obtained under the condition of reducing the use of a surfactant, and the product has the functions of sterilization, moisture preservation and corrosion prevention, and the p-hydroxyacetophenone is a natural plant extract existing in stems and leaves of artemisia scoparia belonging to the family Compositae, roots of artemisia capillaris, plants belonging to the family Asclepiadaceae, tylophora japonica and the like, has no harm to the skin, and is a safe high-temperature auxiliary activity stabilizer in the medical field at present.
By adopting the technical scheme, the preparation method of the filtrate containing the fermentation product of the Inonotus obliquus comprises the following steps: s1: preparing a fermentation base liquid: preparing a fermentation base liquid according to a formula table, placing the fermentation base liquid in a fermentation container, sealing the container, sterilizing the base liquid, and cooling the fermentation base liquid to room temperature; s2: inoculating strains: inoculating seed liquid into the fermentation base liquid of S1, waiting for fermentation, sealing the mouth of the fermentation container of S1 by a sealing cover, wherein the inoculation amount of the seed liquid is 1-5% of the volume of the fermentation base liquid; s3: and (3) fermentation process: placing the container containing the liquid to be fermented in a mixed culture vessel with the humidity of 30-60%, the temperature of 25-32 ℃, the rotating speed of 130-; and sterile air is introduced into the fermentation container during fermentation.
By adopting the technical scheme, the sterilization of the base liquid in the step S1 adopts a damp-heat sterilization method in a heating sterilization method, and the fermentation container is put into a high-pressure steam sterilizer for sterilization at 121 ℃ for 30 min.
By adopting the technical scheme, the formula of the culture medium in the step S1 takes the extract of the truffle and the inonotus obliquus as a solvent, and the formula is as follows:
by adopting the technical scheme, the truffle and chaga extract and the rice milk containing a large amount of natural components are added into the culture medium, and then the culture medium is sterilized, fermented, cooled to room temperature, inoculated with strains, and finally placed into a mixed culture vessel for mixed culture, and suitable humidity and temperature are provided, and continuous mixing is carried out, so that the flora is more closely fermented.
Preferably, the preparation method of the extract of chaga of truffle in step S1 includes: pulverizing selected truffle and Chaba, sieving with 40-100 mesh sieve, and mixing the sieved powder to obtain powder; the content of truffle in the mixed powder is 40-60 percent, and the rest is the chaga; the weight ratio of the mixed powder to the water is (10-25): (90-120), heating at 75-90 deg.C for 12-18h, cooling to room temperature, and filtering to obtain extractive solution.
According to the technical scheme, the rice milk is prepared by soaking selected rice in water for 12-18 hours according to the mass ratio of the rice to the water of 1-2: 1-4, then grinding, and filtering a product by using gauze of 40-100 meshes to obtain the required rice milk.
By adopting the technical scheme, the proportion of the water of the rice can be controlled, so that the nutrient components in the rice can be prevented from being damaged by high temperature during grinding, and the stability of the rice milk can be ensured to a greater extent. Improving the nutrient richness of the culture.
By adopting the technical scheme, the seed solution in the step S2 is dried powder of Saccharomyces cerevisiae (with the preservation number of CICC 1001) purchased from China Industrial microorganism culture Collection center, and is added into a prepared YPD culture medium to be cultured for 8 hours, so as to obtain the fermented seeds.
By adopting the technical scheme, the seed solution in the step S2 is prepared by adding dry powder of Pediococcus acidilactici (with the preservation number of CICC 10344) and Bifidobacterium animalis subsp.lactis (with the preservation number of CICC 21712) purchased from China Industrial microbiological culture Collection center into a prepared MRS culture medium for culturing for 8 hours to obtain the fermented seeds.
By adopting the technical scheme, the preparation process of the filtrate of the mixed fermentation product containing the Fuscoporia Obliqua and the lactic acid bacteria bifidobacterium comprises the following steps: q1: preparing a fermentation base liquid: preparing a fermentation base liquid, placing the fermentation base liquid in a fermentation container, sealing the container, sterilizing the base liquid, and then cooling the fermentation base liquid to room temperature; q2: inoculating strains: inoculating seed liquid into the fermentation base liquid Q1, and waiting for fermentation, wherein the inoculation amount of the seed liquid is 1-5% of the volume of the fermentation base liquid; q3: and (3) fermentation process: placing the container containing the liquid to be fermented in a mixed culture vessel with the humidity of 30-60%, the temperature of 30-40 ℃, the rotating speed of 130-; the fermentation container is used for anaerobic fermentation during fermentation, and the anaerobic environment is strictly controlled.
The sterilization of the base liquid in the step Q1 adopts a damp-heat sterilization method in a heating sterilization method, and the fermentation container is put into a high-pressure steam sterilizer for sterilization at 121 ℃ for 30 min.
By adopting the technical scheme, the formula of the culture medium in the step Q1 is a truffle and chaga extract solvent, and the formula is as follows:
the preparation process of the soybean milk comprises the following steps of according to the mass ratio of soybean to water (1-2): (1-4) soaking the selected soybeans in water for 12-18 hours, grinding the soybeans until the time is up, and filtering the product by using gauze of 40-100 meshes to obtain the required soybean milk.
By adopting the technical scheme, the truffle and chaga extracting solution adopts a mode of mixing truffle and chaga, so that active substances can be richer, and the truffle and the chaga are heated and boiled at a high temperature for a long time to fully transfer components in the truffle and the chaga into water.
Similarly, in the step Q1 of preparing the filtrate of the mixed fermentation product of the Fuscoporia Obliqua and the Bifidobacterium, the soybeans in the culture medium are also in the form of soybean milk.
By adopting the technical scheme, the mixed culture vessel adopted in the steps S3 and Q3 is one of a shaking table, a vibrator, a blending machine and a stirrer.
The cosmetic raw material composition containing the filtrate of the truffle and inonotus obliquus fermentation product disclosed by the invention can be applied to smoothing toner, emulsion, essence or cream.
Compared with the prior art, the invention has the technical effects that: the invention introduces the truffle and the inonotus obliquus fermentation product into the field of cosmetics, enlarges the application range of the truffle and the inonotus obliquus, the main material of the invention is prepared by natural fermentation, has the characteristics of safety and environmental protection, and simultaneously, the truffle and the inonotus obliquus mixed substrate is adopted for fermentation, so that the active material is richer. The truffle and chaga fermentation product contains polypeptide, amino acid, organic acid, vitamin and other substances, and can improve skin quality, promote metabolism, further relieve skin problems caused by skin surface ecological balance, and achieve the effect of skin care.
Drawings
FIG. 1 is a graph of the clearance of the product free radical (DPPH) scavenging experiment of the present invention.
Detailed Description
The present invention will be described in further detail with reference to the following drawings and examples. It should be understood that the specific embodiments described herein are merely illustrative of the invention and are not intended to limit the invention.
Example 1
A preparation method and application of a cosmetic raw material composition containing a filtrate of a truffle and inonotus obliquus fermentation product are characterized in that: the cosmetic raw material composition contains 0.5% of 1, 2-hexanediol, 0.3% of p-hydroxyacetophenone, 80% of filtrate containing fermentation product of Chaba Tricholoma matsutake, and the rest is filtrate containing mixed fermentation product of Chaba Lactobacillus and Bifidobacterium. Simultaneously comprises the following steps:
pulverizing selected truffle and Chaba, sieving with 40 mesh sieve, and mixing the sieved powder to obtain powder; the content of truffle in the mixed powder is 40 percent, and the rest is inonotus obliquus; the weight ratio of the mixed powder to water is 10: heating at 90 deg.C and 75 deg.C for 12 deg.C, cooling to room temperature, and filtering to obtain extractive solution.
According to the mass ratio of rice to water of 1:2, soaking the selected rice in water for 12 hours, grinding the rice till the time, and filtering the product by a 40-mesh gauze to obtain the required rice pulp.
Soaking the selected soybeans in water for 12 hours according to the mass ratio of the soybeans to the water of 1:2, grinding the soybeans at the time, and filtering the product with 40-mesh gauze to obtain the required soybean milk.
The preparation of the filtrate containing the fermentation product of the truffle white birch mushroom yeast comprises the following steps: s1: preparing a fermentation base liquid: preparing a fermentation base liquid according to a formula table, placing the fermentation base liquid in a fermentation container, sealing the container, sterilizing the base liquid, and cooling the fermentation base liquid to room temperature; s2: inoculating strains: inoculating seed liquid into the fermentation base liquid in the step S1, waiting for fermentation, and sealing the opening of the fermentation container in the step S1 by using a sealing cover, wherein the inoculation amount of the seed liquid is 1% of the volume of the fermentation base liquid; s3: and (3) fermentation process: placing the container containing the liquid to be fermented in a mixed culture vessel with humidity of 30%, temperature of 25 deg.C and rotation speed of 130r/min, fermenting and culturing for 3-5 days to obtain stock solution containing fermentation product of Tricholoma matsutake Singer, and filtering to obtain filtrate containing fermentation product of Tricholoma matsutake Singer; and sterile air is introduced into the fermentation container during fermentation.
The preparation of the filtrate of the mixed fermentation product containing the truffle and inonotus obliquus and bifidobacterium, which comprises the following steps: q1: preparing a fermentation base liquid: preparing a fermentation base liquid according to a formula table, placing the fermentation base liquid in a fermentation container, sealing the container, sterilizing the base liquid, and cooling the fermentation base liquid to room temperature; q2: inoculating strains: inoculating seed liquid into the fermentation base liquid Q1, and waiting for fermentation, wherein the inoculation amount of the seed liquid is 1 of the volume of the fermentation base liquid; s3: and (3) fermentation process: placing the container containing the liquid to be fermented in a mixed culture vessel with the humidity of 30%, the temperature of 30 ℃ and the rotating speed of 130r/min for fermentation culture for 3 days, wherein the obtained liquid is a stock solution of a mixed fermentation product containing the Fuscoporia Obliqua and the Bifidobacterium, and filtering to obtain a filtrate of the mixed fermentation product containing the Fuscoporia Obliqua and the Bifidobacterium; the fermentation container is used for anaerobic fermentation during fermentation, and the anaerobic environment is strictly controlled.
And finally, compounding the filtrate of the fermentation product of the Inonotus obliquus, the filtrate of the mixed fermentation product of the bifidobacterium containing the Inonotus obliquus fermented lactic acid bacteria, 1, 2-hexanediol and p-hydroxyacetophenone to prepare the finished product.
The cosmetic raw materials comprise, but are not limited to, a filtrate of a fermentation product of the inonotus obliquus, a filtrate of a mixed fermentation product of bifidobacterium containing the inonotus obliquus, 1, 2-hexanediol and p-hydroxyacetophenone.
The cosmetic raw material composition is used in essence cosmetics, and has the following formula:
example 2
A preparation method and application of a cosmetic raw material composition containing a filtrate of a truffle and inonotus obliquus fermentation product are characterized in that: the cosmetic raw material composition contains 0.6% of 1, 2-hexanediol, 0.5% of p-hydroxyacetophenone, 85% of filtrate containing fermentation product of Chaba Tricholoma matsutake, and the rest filtrate containing mixed fermentation product of Chaba Lactobacillus and Bifidobacterium. Simultaneously comprises the following steps:
pulverizing selected truffle and Chaba, sieving with 60 mesh sieve, and mixing the sieved powder to obtain powder; the content of truffle in the mixed powder is 45 percent, and the rest is inonotus obliquus; the weight ratio of the mixed powder to water is 10: heating at 90 deg.C and 75 deg.C for 14 deg.C, cooling to room temperature, and filtering to obtain extractive solution.
According to the mass ratio of rice to water of 1:3, soaking the selected rice in water for 14 hours, grinding the rice till the time, and filtering the product by a 40-mesh gauze to obtain the required rice pulp.
Soaking the selected soybeans in water for 14 hours according to the mass ratio of the soybeans to the water of 1:3, grinding the soybeans until the time comes, and filtering the product with 40-mesh gauze to obtain the required soybean milk.
The preparation of the filtrate containing the fermentation product of the truffle white birch mushroom yeast comprises the following steps: s1: preparing a fermentation base liquid: preparing a fermentation base liquid according to a formula table, placing the fermentation base liquid in a fermentation container, sealing the container, sterilizing the base liquid, and cooling the fermentation base liquid to room temperature; s2: inoculating strains: inoculating seed liquid into the fermentation base liquid in the step S1, waiting for fermentation, and sealing the opening of the fermentation container in the step S1 by using a sealing cover, wherein the inoculation amount of the seed liquid is 2% of the volume of the fermentation base liquid; s3: and (3) fermentation process: placing the container containing the liquid to be fermented in a mixed culture vessel with humidity of 30%, temperature of 25 deg.C and rotation speed of 130r/min, fermenting and culturing for 3 days to obtain stock solution containing fermentation product of Tricholoma matsutake Singer, and filtering to obtain filtrate containing fermentation product of Tricholoma matsutake Singer; and sterile air is introduced into the fermentation container during fermentation.
The preparation of the filtrate of the mixed fermentation product containing the truffle and inonotus obliquus and bifidobacterium, which comprises the following steps: q1: preparing a fermentation base liquid: preparing a fermentation base liquid according to a formula table, placing the fermentation base liquid in a fermentation container, sealing the container, sterilizing the base liquid, and cooling the fermentation base liquid to room temperature; q2: inoculating strains: inoculating seed liquid into the fermentation base liquid Q1, and waiting for fermentation, wherein the inoculation amount of the seed liquid is 2% of the volume of the fermentation base liquid; s3: and (3) fermentation process: placing the container containing the liquid to be fermented in a mixed culture vessel with humidity of 30%, temperature of 32 deg.C and rotation speed of 130r/min, fermenting and culturing for 3 days to obtain a liquid, i.e. stock solution of mixed fermentation product containing Fuscoporia Obliqua and Bifidobacterium, and filtering to obtain filtrate of mixed fermentation product containing Fuscoporia Obliqua and Bifidobacterium; the fermentation container is used for anaerobic fermentation during fermentation, and the anaerobic environment is strictly controlled.
And finally, compounding the filtrate of the fermentation product of the Inonotus obliquus, the filtrate of the mixed fermentation product of the bifidobacterium containing the Inonotus obliquus fermented lactic acid bacteria, 1, 2-hexanediol and p-hydroxyacetophenone to prepare the finished product.
The cosmetic raw materials comprise, but are not limited to, a filtrate of a fermentation product of the inonotus obliquus, a filtrate of a mixed fermentation product of bifidobacterium containing the inonotus obliquus, 1, 2-hexanediol and p-hydroxyacetophenone.
The cosmetic raw material is used in the dairy cosmetics, and the formula is as follows:
example 3
A preparation method and application of a cosmetic raw material composition containing a filtrate of a truffle and inonotus obliquus fermentation product are characterized in that: the cosmetic raw material composition contains 0.7% of 1, 2-hexanediol, 1% of p-hydroxyacetophenone, 90% of filtrate containing fermentation product of Chaba yeast, and the rest is filtrate containing mixed fermentation product of Chaba lactobacillus and bifidobacterium. Simultaneously comprises the following steps:
pulverizing selected truffle and Chaba, sieving with 80 mesh sieve, and mixing the sieved powder uniformly to obtain powder; the content of truffle in the mixed powder is 50 percent, and the rest is inonotus obliquus; the weight ratio of the mixed powder to water is 10: heating at 120 deg.C and 75 deg.C for 16 deg.C, cooling to room temperature, and filtering to obtain extractive solution.
According to the mass ratio of rice to water of 1:4 soaking the selected rice in water for 16 hours, grinding the rice at the time, and filtering the product by a 40-mesh gauze to obtain the required rice pulp.
Soaking the selected soybeans in water for 16 hours according to the mass ratio of the soybeans to the water of 1:4, grinding at the time, and filtering the product by using 40-mesh gauze to obtain the required soybean milk.
The preparation of the filtrate containing the fermentation product of the truffle white birch mushroom yeast comprises the following steps: s1: preparing a fermentation base liquid: preparing a fermentation base liquid according to a formula table, placing the fermentation base liquid in a fermentation container, sealing the container, sterilizing the base liquid, and cooling the fermentation base liquid to room temperature; s2: inoculating strains: inoculating seed liquid into the fermentation base liquid in the step S1, waiting for fermentation, and sealing the opening of the fermentation container in the step S1 by using a sealing cover, wherein the inoculation amount of the seed liquid is 2% of the volume of the fermentation base liquid; s3: and (3) fermentation process: placing the container containing the liquid to be fermented in a mixed culture vessel with humidity of 40%, temperature of 30 deg.C and rotation speed of 200r/min, fermenting and culturing for 5 days to obtain stock solution containing fermentation product of Tricholoma matsutake Singer, and filtering to obtain filtrate containing fermentation product of Tricholoma matsutake Singer; and sterile air is introduced into the fermentation container during fermentation.
The preparation of the filtrate of the mixed fermentation product containing the truffle and inonotus obliquus and bifidobacterium, which comprises the following steps: q1: preparing a fermentation base liquid: preparing a fermentation base liquid according to a formula table, placing the fermentation base liquid in a fermentation container, sealing the container, sterilizing the base liquid, and cooling the fermentation base liquid to room temperature; q2: inoculating strains: inoculating seed liquid into the fermentation base liquid Q1, and waiting for fermentation, wherein the inoculation amount of the seed liquid is 2% of the volume of the fermentation base liquid; s3: and (3) fermentation process: placing the container containing the liquid to be fermented in a mixed culture vessel with the humidity of 40-60%, the temperature of 30 ℃ and the rotating speed of 200r/min for fermentation culture for 5 days, wherein the obtained liquid is a stock solution of a mixed fermentation product containing the Fuscoporia Obliqua and the Bifidobacterium, and filtering to obtain a filtrate of the mixed fermentation product containing the Fuscoporia Obliqua and the Bifidobacterium; the fermentation container is used for anaerobic fermentation during fermentation, and the anaerobic environment is strictly controlled.
And finally, compounding the filtrate of the fermentation product of the Inonotus obliquus, the filtrate of the mixed fermentation product of the bifidobacterium containing the Inonotus obliquus fermented lactic acid bacteria, 1, 2-hexanediol and p-hydroxyacetophenone to prepare the finished product.
The cosmetic raw materials comprise, but are not limited to, a filtrate of a fermentation product of the inonotus obliquus, a filtrate of a mixed fermentation product of bifidobacterium containing the inonotus obliquus, 1, 2-hexanediol and p-hydroxyacetophenone.
The cosmetic raw material is used in the cream cosmetics, and the formula is as follows:
the skin irritation test of the cosmetic raw material composition of the present invention was as follows:
procedure of experiment
The subjects (average age 25 years, 24-27 years) were subjected to skin patch test (patch test) on the forearm using Finn Chamber. However, patients with psoriasis (psoriasis), eczema (rash) or other skin lesions who are pregnant, lactating or taking contraceptives, antihistamines etc. cannot participate in this study. After wiping the test site with 70% ethanol and drying, 25-30 μ L of torreya grandis complex essential oil sample was added to the finnish chamber, and the finnish chamber was fixed to the forearm area without water. Removing the patch after 24 h; the test site was marked with a marking pen and after 30 minutes, the test site was again observed 24 hours later and the tester information was recorded in detail. The degree of irritation was classified according to the criteria of ((International Contact Dermatitis Research Group: ICDRG), and was determined 30 minutes after removal of the patch, again after 24 hours, and after determination according to the criteria of the International Research on Contact Dermatitis for skin reactions (Table 1), the average score was estimated according to the formula of the average score of skin reactions [ Table 2], and whether irritation was observed or not was determined according to the result of the score in the skin patch test (patch test).
TABLE 1 International Research Group of Contact Dermatitis ((ICDRG)
And (3) judging standard:
the standard for judging skin reaction
1) Negative (-): has no irritation
2) Double or slice interaction and erythrema (+ -); microstimulation, faint barely visible mild erythema
3) Erythema + Industration (+); slight irritation, marked erythema, and skin sclerosis
4) Erythema + Industration + Vesicle (++); moderate irritation, erythema, cirrhosis, vesicles
5) Erythema + Industration + Bullae (++); strong irritation, erythema, sclerosis, and blister
Equation for calculating average skin reaction score Mean score
Maximum score: full response: number of respondents No. of total subjects: total number of people
No. of evaluation: evaluation number mean score: average score
i-results of respective scores obtained by removing the patch and measuring the site after 30 minutes and 24 hours according to the ICDRG standard. The markers erythema (erythema) and edema (edema) are both reactions, and are all applicable.
j-removal of the patch, 30 minutes later, 24 hours later, the respective skin responded to the number of testers, marking both the erythema and edema responses, and all were applicable.
TABLE 2 skin Patch test results
| Determination (Grade) | MeanScore |
| Without irritation (1) | 0.00～0.75 |
| Micro-stimulation (2) | 0.76～1.50 |
| Light stimulus (3) | 1.51～2.50 |
| Middle stimulus (4) | 2.51～4.00 |
| Strong stimulation (5) | 4.01 |
As a result: (ICDRG Score Total)
And (4) experimental conclusion:
the antibacterial experiment of the cosmetic raw material of the invention is as follows:
the experimental steps are as follows:
(1) obtaining bacterial liquid: refrigerator for storing strains
(2) Preparation of experimental sample bacterial liquid
With 0.85% NaCl as negative control, 5 EP tubes per group were added with the bacterial solution, and left overnight at 27 ℃ for treatment on the bright day.
(3) Determination of dilution concentration
Taking a saline group, diluting the saline group to 10^1, 10^2, 10^3 and 10^4 times by using 0.85% NaCl, taking 0.01ml of the saline group by using a pipette, spotting the saline group on a corresponding culture medium, culturing the saline group in an inverted manner at the temperature of 27 ℃ for 1d, counting the number of colonies, and taking the dilution factor of the number of the colonies between 10 and 50 as the experimental dilution factor. A. niger has long growth cycle, and 10^3 times is selected as the dilution factor.
(4) And (3) treatment of bacteria:
after processing for 1d, diluting each group of sample bacterial liquid to an experimental dilution factor (stock solution, 10^1, 10^2-10^ n), taking 0.01ml of sample bacterial liquid to be spotted on a corresponding culture medium, marking, culturing for 1-3d at 27 ℃, observing the growth condition of bacterial colonies, counting the number of the bacterial colonies and recording; taking 0.05ml of the culture medium, dotting the culture medium on the culture medium, marking, culturing at 27 ℃ for 1-3d, observing the growth condition of colonies, counting the number of the colonies and recording; calculation of bacterial inhibition (%)
Inhibition (%) formula (1-colony count cfu/ml/0.85% NaCl/colony count) 100%
And (5) if the inhibition rate (%) does not reach the standard, processing for 3d, 7d and the like, and repeating the step (4).
The experimental results are as follows:
1d
3d
note: coli (escherichia coli) s.aure (staphylococcus aureus) p.aeru (bacillus aeruginosa) c.albican (candida albicans) a.niger (aspergillus niger)
The cosmetic raw material composition of the present invention, which scavenges DPPH radicals, was tested as follows:
first, reagent preparation
(1)3.943mgDPPH was made up to 50mL with 95% ethanol
(2)10mgTrolox is fixed to 100mL by 95% ethanol
(3) The invention
Second, the experimental procedure
(1) UV zero calibration, 95% ethanol as blank
(2) Preliminary experiments (DPPH concentration selection)
Shaking 500 μ L DPPH +1000 μ L95% ethanol for 10s, standing for 10min, and measuring absorbance (absorbance at 520nm of 0.45-0.55, can be used as DPPH solution for the following experiment)
(3) Determination of Trolox
| Sample | Trolox(μL) | 95% ethanol (μ L) | DPPH(μL) |
| S | 100 | 900 | 500 |
| SB | 100 | 900+500 | 0 |
| C | 0 | 100+900 | 500 |
| CB | 0 | 100+900+500 | 0 |
Adding DPPH or 95% ethanol, shaking for 10s, standing for 10min, and measuring absorbance
(4) Experimental procedure
(5) The absorbance values were measured at 520nm, tabulated and plotted to obtain IC50 values.
Calculating the formula: inhibition rate (%) [1- (S-SB)/(C-CB) ]. 100
Third, experimental results
| Inner(μL) | Con(μL/mL) | DPPH(％) |
| 400 | 266.67 | 9.40 |
| 300 | 200 | 9.27 |
| 200 | 133.33 | 6.30 |
The DPPH radical scavenging test junction of the present invention is shown in FIG. 1.
The DPPH clearance rate is increased with the increase of the addition amount of the compound in the experiment process. When the addition amount is 200ul-300ul, the DPPH clearance rate increasing effect is obvious.
The natural skin-friendly antibacterial and antioxidant cosmetic is prepared by natural fermentation, is mild and non-irritant to skin, is suitable for wide groups, has good antibacterial and antioxidant effects, can reduce the use of preservatives in cosmetics, can make the cosmetics milder, increases the safety and the acceptance of the public on the cosmetics, and has extremely high economic value.
While the foregoing description shows and describes the preferred embodiments of the present invention, it is to be understood that the invention is not limited to the forms disclosed herein, but is not to be construed as excluding other embodiments and is capable of use in various other combinations, modifications, and environments and is capable of changes within the scope of the inventive concept as described herein, commensurate with the above teachings, or the skill or knowledge of the relevant art. And that modifications and variations may be effected by those skilled in the art without departing from the spirit and scope of the invention as defined by the appended claims.
Claims (10)
1. A cosmetic raw material composition containing a filtrate of a truffle and inonotus obliquus fermentation product is characterized in that: the cosmetic raw material composition comprises the following components: filtrate containing fermentation product of Chaba Tricholoma matsutake, filtrate containing mixed fermentation product of Bacillus bifidus of Chaba Tricholoma matsutake, 1, 2-hexanediol, and p-hydroxyacetophenone; and the content of the 1, 2-hexanediol is 0.5-2%, the content of the p-hydroxyacetophenone is 0.1-1%, the filtrate of the fermentation product containing the chaga yeast is 80-90%, and the filtrate of the mixed fermentation product containing the chaga lactobacillus and bifidobacterium is remained.
2. The cosmetic raw material composition comprising the filtrate of the fermentation product of Chaba of Pinus koraiensis according to claim 1, wherein the composition is applied to a smoothing toner, a lotion, a essence or a cream.
3. A method for preparing a cosmetic raw material composition containing a filtrate of a truffle and inonotus obliquus fermentation product is characterized by comprising the following steps: comprises the preparation of filtrate containing fermentation product of the white birch mushroom yeast, which comprises the following steps: s1: preparing a fermentation base liquid: preparing a fermentation base liquid, placing the fermentation base liquid in a fermentation container, sealing the container, sterilizing the base liquid, and then cooling the fermentation base liquid to room temperature; s2: inoculating strains: inoculating seed liquid into the fermentation base liquid of S1, waiting for fermentation, sealing the mouth of the fermentation container of S1 by a sealing cover, wherein the inoculation amount of the seed liquid is 1-5% of the volume of the fermentation base liquid; s3: and (3) fermentation process: placing the container containing the liquid to be fermented in a mixed culture vessel with the humidity of 30-60%, the temperature of 25-32 ℃, the rotating speed of 130-; and sterile air is introduced into the fermentation container during fermentation.
4. The method of preparing a cosmetic raw material composition containing a filtrate of a fermentation product of Chaba of truffle according to claim 3, wherein: the formula of the culture medium in the step S1 takes the extract of the truffle and the inonotus obliquus as a solvent, and the formula is as follows:
5. the method of preparing a cosmetic raw material composition containing a filtrate of a fermentation product of Chaba of Pinus koraiensis according to claim 4, wherein: the preparation method takes the truffle and inonotus obliquus extract as a fermentation base solution solvent as follows: pulverizing selected truffle and Chaba, sieving with 40-100 mesh sieve, and mixing the sieved powder to obtain powder; the content of truffle in the mixed powder is 40-60 percent, and the rest is the chaga; the weight ratio of the mixed powder to water is 10-25: 90-120, the mixture is heated at 75-90 ℃ for 12-18h, cooled to room temperature and filtered to obtain the truffle and chaga extract.
6. The method of preparing a cosmetic raw material composition containing a filtrate of a fermentation product of Chaba of truffle according to claim 3, wherein: the sterilization of the base liquid in the step S1 adopts a damp-heat sterilization method in a heating sterilization method, and the fermentation container is put into a high-pressure steam sterilizer for sterilization at 121 ℃ for 30 min.
7. The method of preparing a cosmetic raw material composition containing a filtrate of a fermentation product of Chaba of Pinus koraiensis according to claim 5, wherein: the preparation process of the rice milk comprises the steps of soaking selected rice in water for 12-18 hours according to the mass ratio of the rice to the water of 1-2: 1-4, then grinding, and filtering a product through 40-100-mesh gauze to obtain the required rice milk.
8. A method for preparing a cosmetic raw material composition containing a filtrate of a truffle and inonotus obliquus fermentation product is characterized by comprising the following steps: the preparation method comprises the preparation of a filtrate containing a mixed fermentation product of the truffle and inonotus obliquus and bifidobacterium, and the preparation process comprises the following steps: q1: preparing a fermentation base liquid: preparing a fermentation base liquid, placing the fermentation base liquid in a fermentation container, sealing the container, sterilizing the base liquid, and then cooling the fermentation base liquid to room temperature; q2: inoculating strains: inoculating seed liquid into the fermentation base liquid Q1, and waiting for fermentation, wherein the inoculation amount of the seed liquid is 1-5% of the volume of the fermentation base liquid; q3: and (3) fermentation process: placing the container containing the liquid to be fermented in a mixed culture vessel with the humidity of 30-60%, the temperature of 30-40 ℃, the rotating speed of 130-; the fermentation container is used for anaerobic fermentation during fermentation, and the anaerobic environment is strictly controlled.
9. The method of preparing a cosmetic raw material composition containing a filtrate of a fermentation product of Chaba of Pinus koraiensis according to claim 8, wherein: the formula of the culture medium in the step Q1 is a truffle inonotus obliquus extract solvent, and the formula is as follows:
10. the method of preparing a cosmetic raw material composition containing the filtrate of the fermentation product of Chaba of Pinus koraiensis according to claim 9, wherein: the preparation process of the soybean milk comprises the following steps of according to the mass ratio of soybean to water (1-2): (1-4) soaking the selected soybeans in water for 12-18 hours, grinding the soybeans until the time is up, and filtering the product by using gauze of 40-100 meshes to obtain the required soybean milk.
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Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN114748381A (en) * | 2022-05-06 | 2022-07-15 | 上海铂舍生物科技有限公司 | Method for preparing truffle extract and fermentation liquor and application |
| CN115554226A (en) * | 2022-11-11 | 2023-01-03 | 广州优科生物科技有限公司 | Multi-strain fermentation filtrate and preparation method and application thereof |
| CN115645313A (en) * | 2022-09-27 | 2023-01-31 | 苏州猫尔科技有限公司 | Hair repairing composition and application thereof |
| CN115737490A (en) * | 2022-11-02 | 2023-03-07 | 稀物集(广州)生物科技有限公司 | Preparation method of Inonotus obliquus yeast fermentation liquor and application of Inonotus obliquus yeast fermentation liquor in cosmetics |
-
2021
- 2021-06-10 CN CN202110648428.7A patent/CN113288830A/en not_active Withdrawn
Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN114748381A (en) * | 2022-05-06 | 2022-07-15 | 上海铂舍生物科技有限公司 | Method for preparing truffle extract and fermentation liquor and application |
| CN115645313A (en) * | 2022-09-27 | 2023-01-31 | 苏州猫尔科技有限公司 | Hair repairing composition and application thereof |
| CN115645313B (en) * | 2022-09-27 | 2023-10-27 | 苏州猫尔科技有限公司 | Hairline repairing composition and application thereof |
| CN115737490A (en) * | 2022-11-02 | 2023-03-07 | 稀物集(广州)生物科技有限公司 | Preparation method of Inonotus obliquus yeast fermentation liquor and application of Inonotus obliquus yeast fermentation liquor in cosmetics |
| CN115554226A (en) * | 2022-11-11 | 2023-01-03 | 广州优科生物科技有限公司 | Multi-strain fermentation filtrate and preparation method and application thereof |
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