CN103392747A - Application of radix paeoniae alba extract - Google Patents
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- CN103392747A CN103392747A CN2013103748594A CN201310374859A CN103392747A CN 103392747 A CN103392747 A CN 103392747A CN 2013103748594 A CN2013103748594 A CN 2013103748594A CN 201310374859 A CN201310374859 A CN 201310374859A CN 103392747 A CN103392747 A CN 103392747A
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Abstract
The invention relates to an application of a radix paeoniae alba extract for controlling grey mould fruit rot of strawberry. The extract has a remarkable inhibiting effect and a certain in-vitro control efficiency to grey mould fruit rot of strawberry by way of inhibiting molecular spore germination, sclerotium germination and the like, and can be further developed to a novel plant source bactericide.
Description
Technical field
The invention belongs to biological technical field, relate generally to the application of radix paeoniae alba extraction, be specifically related to the application of radix paeoniae alba extraction in controlling grey mould fruit rot of strawberry.
Background technology
Strawberry not only is of high nutritive value, but also can consolidate gums, fresh breath, moist throat, the effect of improving looks and building up resistance.In recent years, along with growth in the living standard, people also increase year by year to the consumption figure of strawberry, and this has brought vigor and vitality for the development of Strawberry industry, but because the culture technique of strawberry is had relatively high expectations, and be not easy to transportation and shift, thereby producing region is more fixing, in a place, repeats for many years to plant strawberry, easily accumulates pathogen, often cause the Diseases of Strawberry occurrence and harm that the trend of increasing the weight of is arranged, wherein grey mould fruit rot of strawberry is exactly one of Major Diseases of harm strawberry production.
Grey mould fruit rot of strawberry (Botrytis cinerea Pers.et Tris.) is a kind of Common Diseases of harm strawberry, and this disease mainly causes fruit rot, to the serious threat of safety in production formation of strawberry, has become the important factor in order of restriction strawberry volume increase.On producing, in order to control the occurrence and harm of grey mould fruit rot of strawberry, main by methods such as plantation disease-resistant variety, agricultural cultivation control, chemical control and biological controls.Because gray mold is a kind of facultative parasite, also there is the saprophytic phase, and also do not find now its antigen, make breeding for disease resistance be difficult to carry out; The anti-rules for the treatment of of agricultural cultivation mainly, by strengthening the water and fertilizer management to field, regulate temperature, humidity and illumination etc. and reach the purpose that reduces disease, but it are not obvious to produce effects.Adopt chemical control to remain the Main Means of the careless malicious gray mold of China's control, but owing to using continuously chemical agent, particularly use continuously single medicament, cause the gray mold germ to produce gradually pesticide resistance, the control efficiency of chemical pesticide also descends year by year, and pesticide spraying can't be avoided directly being sprayed onto on fruit surface during dispenser, has polluted fruit, easily causes poisoning to occur.Nearest result of study shows, adopts that the biological control grey mould fruit rot of strawberry not only can reach efficiently, safety, environmental protection, and is pollution-free, and effect is better, wherein, from plant, finding new bactericidal active substance, develop the novel plant source bactericide, become one of focus of present people's concern.
The root of herbaceous peony is the dry root of the peeling of ranunculaceae plant Chinese herbaceous peony, and due to its cold nature, bitter, acid, return liver, the spleen channel, the effect of the flat liver pain relieving of tool, nourishing blood for regulating menstruation, astringing YIN to stop sweating, can be used for that treatment headache is dizzy, hypochondriac pain, stomachache, four limbs contraction pain, and the deficiency of blood is sallow, irregular menstruation, spontaneous perspiration, night sweat etc.Existing result of study shows: the root of herbaceous peony also has anti-inflammatory, eases pain, protects the effects such as liver, immunological regulation.Up-to-date result of study demonstration, radix paeoniae alba extraction is to pathogen, and is as also inhibited as malignant bacterias such as Escherichia coli, staphylococcus aureuses.As can be seen here, the medical value of radix paeoniae alba extraction is very extensive, and studies to obtain also comparison system, but this research that is extracted in control agricultural plant disease aspect has no research.In general, different with the object that plant is had to pathogenic microorganism effect owing to animal (people or beast) being had to the microorganism of pathogenic effects, pathogenic mode is also different, though the medicament that uses is inhibited to animal pathogen, may not be inhibited to plant pathogen.Forefathers' result of study shows: radix paeoniae alba extraction has certain inhibitory action to animal pathogen, but to the rarely seen report of the inhibiting research of phytopathogen.The present invention, according to this problem, has carried out Primary Study to the research of root of herbaceous peony retrieval inhibition agricultural plant disease-grey mould fruit rot of strawberry, and being intended to provides theoretical foundation for the prevention and control grey mould fruit rot of strawberry.
Summary of the invention
Technical problem to be solved by this invention is: for above-mentioned the deficiencies in the prior art, provide the control application of a kind of radix paeoniae alba extraction to phytopathogen.
In order to solve the problems of the technologies described above, the technical solution adopted in the present invention is: the application of a kind of radix paeoniae alba extraction in controlling grey mould fruit rot of strawberry.
Above-mentioned radix paeoniae alba extraction is, after getting root of herbaceous peony piece root and shining dry grinding, with the analysis pure acetone, White Peony Root to be carried out to lixiviate, gained filtrate evaporation and concentration and obtaining.Its concrete preparation method is as follows:
A, get root of herbaceous peony piece root dry to water content be 2.5-4.5%, with the rotating speed of 6500-8500r/min, be crushed to 95-115 order fine powder.
B, with analyzing pure acetone, White Peony Root is carried out to lixiviate, White Peony Root is disposable to add, acetone divides 3 times and adds, after the ratio that at every turn adds 30-50mL acetone in the 1g White Peony Root adds, in on shaking table under 20-30 ℃ with 100-140r/min jolting 48-60h, filter, collect filtrate, merge 3 times filtrate;
C, gained filtrate being inserted to rotation concentration and evaporation instrument, is that 45-55 ℃ and condensation temperature are that under 4-6 ℃ of condition, 110-150r/min is concentrated into paste at 0.08-0.1MPa air pressure, bath temperature.
Be only below a preparation method of the radix paeoniae alba extraction that exemplifies of the present invention, this extract can adopt prior art to be prepared in fact.
Radix paeoniae alba extraction of the present invention is by suppressing the modes such as conidia germination, Microsclerotia sprouting, inhibited to the Botrytis cinerea germ, to preventing and treating stripped From Strawberry Leaves and fruit gray mold, have certain effect, and extracting method is simple, quick, easy to operate, easily promotion and application; The required material of separating method is easily bought, and separating rate is fast, and is free from environmental pollution, to person poultry safety's environmental protection, saves cost, economizes on resources.
The accompanying drawing explanation
Fig. 1 is the stripped control efficiency figure of radix paeoniae alba extraction to the From Strawberry Leaves gray mold;
Wherein: 1 represents the radix paeoniae alba extraction processing; 2 represent the processing of carbendazim liquid; 3 represent the sterile water processing.
Fig. 2 is the stripped control efficiency figure of radix paeoniae alba extraction to the strawberry fruit gray mold;
Wherein: 1 represents the radix paeoniae alba extraction processing; 2 represent the processing of carbendazim liquid; 3 represent the sterile water processing.
Embodiment
Below in conjunction with concrete test and embodiment, technical scheme of the present invention is described further.
Raw material root of herbaceous peony piece root used in the present invention can or be bought the production technology maturation by plantation; The raw material supply abundance, the acquisition approach is various.
The pathogen species Botrytis cinerea germ of using in embodiment (Botrytis cinerea Pers.et Tris.) is provided by Agricultural University Of Hunan's plant pathology laboratory.The activation of this bacterial classification: adopt oese scraping 3 ring Botrytis cinerea germ bacterial classifications access potato dextrose agar slant mediums, cultivate 24-48h under 25-28 ℃.
Get after root of herbaceous peony piece root is cleaned and dry, with Universalpulverizer, with the rotating speed of 6500r/min, be crushed to 95 order fine powders, the acquisition White Peony Root.Getting White Peony Root 10g packs in triangular flask, with analyzing pure acetone, White Peony Root is carried out to lixiviate, acetone divides 3 times and adds, after adding 30mL at every turn, put triangular flask on shaking table under 20 ℃ with 100r/min jolting 60h, filter, collect filtrate, merge 3 times filtrate, filtrate is packed into and rotated the concentration and evaporation instrument, at 0.08MPa air pressure, bath temperature, be that 45 ℃ and condensation temperature are under 4 ℃ of conditions, the 110r/min evaporation and concentration, to paste, obtains yellowing look radix paeoniae alba extraction.
Get after the root of herbaceous peony is cleaned and dry, with Universalpulverizer, with the rotating speed of 7500r/min, be crushed to 105 order fine powders, the acquisition White Peony Root.Getting White Peony Root 10g packs in triangular flask, with analyzing pure acetone, White Peony Root is carried out to lixiviate, acetone divides 3 times and adds, after adding 40mL at every turn, put triangular flask on shaking table under 25 ℃ with 120r/min jolting 54h, filter, collect filtrate, merge 3 times filtrate, filtrate is inserted to rotation concentration and evaporation instrument, at 0.09MPa air pressure, bath temperature, be that 50 ℃ and condensation temperature are under 5 ℃ of conditions, the 130r/min evaporation and concentration, to paste, obtains yellowing look radix paeoniae alba extraction.
Get after the root of herbaceous peony is cleaned and dry, with Universalpulverizer, with the rotating speed of 8500r/min, be crushed to 115 order fine powders, the acquisition White Peony Root.Getting White Peony Root 10g packs in triangular flask, with analyzing pure acetone, White Peony Root is carried out to lixiviate, acetone divides 3 times and adds, after adding 50mL at every turn, put triangular flask on shaking table under 30 ℃ with 150r/min jolting 48h, filter, collect filtrate, merge 3 times filtrate, filtrate is inserted to rotation concentration and evaporation instrument, at 0.1MPa air pressure, bath temperature, be that 55 ℃ and condensation temperature are under 6 ℃ of conditions, the 150r/min evaporation and concentration, to paste, obtains yellowing look radix paeoniae alba extraction.
The inhibition of example 4 radix paeoniae alba extractions to the Botrytis cinerea germ
The radix paeoniae alba extraction of getting above-mentioned preparation is packed in triangular flask, with after a small amount of acetone solution, with sterile water, being diluted to concentration again, be 40mg/mL solution, get this solution of 1mL and mix with 9mL potato dextrose agar (adding 30 μ g/mL streptomycins), shake up and make flat board.The inoculation diameter is the grey mould fruit rot of strawberry germ bacterium cake horizontalization plate central authorities of 5mm, with carbendazim (98% carbendazim active compound dilutes with sterile water after a small amount of 0.1mol/L diluted hydrochloric acid dissolution), do the medicament contrast simultaneously, sterile water is made blank, every processing repeats 3 times, under 28-30 ℃, cultivate, after 48h, adopt the right-angled intersection method to measure colony diameter, calculate inhibiting rate, the results are shown in following table 1.Bacteriostasis rate (%)=[(control group colony diameter-processed group colony diameter)/(control group colony diameter-5)] * 100%.
Results and analysis: radix paeoniae alba extraction of the present invention has certain inhibition to the Botrytis cinerea germ.The indoor antibacterial test result shows: after cultivating 48h, radix paeoniae alba extraction of the present invention is 61.92% to Botrytis cinerea germ inhibiting rate, and is suitable with the inhibition that contrasts the medicament carbendazim.The results of analysis of variance also shows: radix paeoniae alba extraction is processed and contrast medicament carbendazim is processed without significant difference (table 1).Illustrate that radix paeoniae alba extraction of the present invention has the potentiality of further exploitation bactericide.
The inhibitory action of table 1 radix paeoniae alba extraction of the present invention to the Botrytis cinerea germ
Annotate: different lowercases represent significant difference (p<0.05), and different capitalizations represent utmost point significant difference (p<0.01).
The impact of example 5 radix paeoniae alba extractions on the spore germination of grey mould fruit rot of strawberry bacterium molecule
Adopt slide sprouting method to detect the impact of radix paeoniae alba extraction on Botrytis cinerea germ conidia germination.At first with a small amount of acetone solution radix paeoniae alba extraction, with the sterile water dilution, being 35mg/mL solution again, simultaneously, Botrytis cinerea germ bacterium cake being placed on the potato dextrose agar flat board and cultivating 96h, after conidium grows, is 1 * 10 with the sterile water dilution
6The spore suspension of individual/mL, the conidial suspension of getting equivalent mixes rear dripping on slide with the solution of aforementioned dilution, take carbendazim as the contrast medicament, and establish blank, repeat 3 times, 48h is cultivated in moisturizing in culture dish, examines under a microscope the conidia germination situation, calculate spore germination rate, see the following form 2.
The impact of table 2 radix paeoniae alba extraction on spore germination
Annotate: different lowercases represent significant difference (p<0.05), and different capitalizations represent utmost point significant difference (p<0.01).
Results and analysis: the conidia germination rate of processing through radix paeoniae alba extraction is lower than the germination rate of blank, but the germination rate higher than the processing of contrast medicament carbendazim, wherein the conidia germination rate processed of radix paeoniae alba extraction is 0.43 times of blank, but contrasts 1.41 times of medicament.The results of analysis of variance shows: radix paeoniae alba extraction process with the processing that contrasts the processing of medicament carbendazim and blank between significant difference not only, and reach utmost point significance level.Illustrate that radix paeoniae alba extraction can reach the pathogenetic purpose of inhibition Botrytis cinerea by suppressing conidia germination.
The impact that example 6 radix paeoniae alba extractions are sprouted Botrytis cinerea germ sclerotium
The Botrytis cinerea germ is inoculated on the potato dextrose agar flat board, cultivates 7d for 20-30 ℃, when the sclerotium blackening is ripe, collect sclerotium stand-by.Sclerotium is immersed in to 48h in the solution that contains series concentration (0.5mg/mL, 5mg/mL, 10mg/mL, 20mg/mL, 40mg/mL) radix paeoniae alba extraction, air-dry being placed on the potato dextrose agar flat board; To with the sclerotium that radix paeoniae alba extraction is processed, not be placed on the potato dextrose agar culture medium flat plate that contains above-mentioned series concentration radix paeoniae alba extraction, 20-30 ℃ of cultivation, every processing repeats 3 times, 100 sclerotium of each replication.Relatively the sclerotium of two kinds of processing is sprouted situation, measures the sclerotium germination rate, sees the following form 3.
Results and analysis: as shown in Table 3, after radix paeoniae alba extraction was processed, sclerotium was sprouted and is affected, and the sclerotium germination rate of wherein processing through radix paeoniae alba extraction is starkly lower than the sclerotium germination rate of processing without radix paeoniae alba extraction.Different radix paeoniae alba extraction concentration and sclerotium germination rate are inverse ratio, and namely along with the raising of radix paeoniae alba extraction concentration, the germination rate of different disposal sclerotium reduces gradually.Illustrate that radix paeoniae alba extraction sprouts and to play inhibitory action Microsclerotia.With the sclerotium that radix paeoniae alba extraction is processed, be not inoculated on the culture medium flat plate that contains the variable concentrations radix paeoniae alba extraction, between each processing, the sprouting situation of sclerotium also has certain difference, and this may vary in size relevant with sclerotium.In addition, the growth of Botrytis cinerea germ mycelia obviously is suppressed when concentration is 40mg/mL, and along with the raising of radix paeoniae alba extraction concentration, inhibition is better.Therefore, radix paeoniae alba extraction not only suppresses the sprouting of sclerotium, and suppresses mycelial growth.
The impact that table 3 radix paeoniae alba extraction is sprouted Botrytis cinerea germ sclerotium
| Concentration (mg/mL) | Process sclerotium germination rate (%) | Untreated sclerotium germination rate (%) |
| 0.5 | 79.14aA | 96.61aA |
| 5 | 73.48bB | 96.40aA |
| 10 | 71.15cC | 95.23bB |
| 20 | 68.45dD | 95.16bBC |
| 40 | 64.7eE | 94.41cC |
Annotate: different lowercases represent significant difference (p<0.05), and different capitalizations represent utmost point significant difference (p<0.01).
The stripped control efficiency of example 7 radix paeoniae alba extractions to the From Strawberry Leaves gray mold
Win growth consistent, without the normal From Strawberry Leaves of damage by disease and insect, first use sterile water wash leaf table impurity, then with 75% alcohol, carry out surface sterilization 4s, then, with in sterile water, washing out residual alcohol, be placed in the vent cabinet natural air drying.Get respectively radix-polygoni multiflori extract, carbendazim medicament, sterile water and evenly be applied in the From Strawberry Leaves tow sides, be placed in the vent cabinet natural air drying, with the card punch of internal diameter 5mm, buy the fresh bacterium cake of getting the botrytis cinerea colony edge that has activated, with oese, choose to blade, during inoculation, avoid bacterium cake contact vein position, smear after complete blade is placed in culture dish, in temperature 26-28 ℃ of lower moisturizing, cultivate 3d, 3 blades of every processing also repeat 3 times, adopt the right-angled intersection method to measure strawberry fruit scab diameter, calculate control efficiency, see the following form 4.
Control efficiency (%)=[(contrast sick spot diameter – and process the scab diameter)/(contrast scab diameter-5)] * 100%.
Results and analysis: as shown in Figure 1, radix paeoniae alba extraction is on the From Strawberry Leaves normal growth without impact, and the scab diameter that ash arrhizus bacteria infects formation after radix paeoniae alba extraction is processed is significantly less than control treatment, but processes greater than contrast medicament carbendazim.By measuring colony diameter, calculate control efficiency and find that radix paeoniae alba extraction can reach 46.28% to the stripped control efficiency of From Strawberry Leaves gray mold, lower than the processing of contrast medicament carbendazim.The results of analysis of variance also shows, radix paeoniae alba extraction process and contrast medicament carbendazim to process difference also remarkable.Above some explanation radix paeoniae alba extraction can be used to prevent and treat grey mould fruit rot of strawberry, and can be by improving concentration or compositely can reaching the purpose of controlling better grey mould fruit rot of strawberry.
The stripped control efficiency of table 4 radix paeoniae alba extraction of the present invention to the From Strawberry Leaves gray mold
Annotate: different lowercases represent significant difference (p<0.05), and different capitalizations represent utmost point significant difference (p<0.01).
The stripped control efficiency of example 8 radix paeoniae alba extractions to the strawberry fruit gray mold
Win fruit size consistent, the strawberry fruit without the maturation of damage by disease and insect, first carry out surface sterilization 4s with 75% alcohol, then use the alcohol of sterile water wash residual, is placed in the vent cabinet natural air drying.Get radix-polygoni multiflori extract and smear with aseptic absorbent cotton at the malicious fruit surface of grass, be placed in the vent cabinet natural air drying.With sterile water, by activating on the botrytis cinerea bacterium colony of 72h the fresh botrytis cinerea spore of giving birth to, wash down, be mixed with 1 * 10
6The concentration of individual/mL, with watering can, at the strawberry fruit base portion, spray the circular spot of 5mm, with carbendazim medicament in contrast, and make blank with sterile water, fruit is placed on to moisturizing in the culture dish that diameter is 15cm and cultivates 48h, 3 fruits of every processing are also repeated 3 times, and utilize moccasin chi right-angled intersection method to measure colony diameter, calculate control efficiency, in Table 5.
Control efficiency (%)=[(contrast colony diameter-processing colony diameter)/(contrast colony diameter-5)] * 100
Results and analysis: as shown in Figure 2, radix paeoniae alba extraction on the strawberry fruit normal growth without impact, and get after thing is processed strawberry fruit is had to certain stripped control efficiency through the root of herbaceous peony, wherein, the From Strawberry Leaves gray mold scab diameter of processing through radix paeoniae alba extraction is less than the processing of blank, but the processing greater than contrast medicament carbendazim, simultaneously, by the right-angled intersection method, measure the scab diameter, calculate control efficiency find radix paeoniae alba extraction to the control efficiency of grey mould fruit rot of strawberry fruit lower than contrast medicament carbendazim.The results of analysis of variance shows: radix paeoniae alba extraction is processed and contrast medicament carbendazim is processed significant difference (table 5).Illustrate that radix paeoniae alba extraction can be used for preventing and treating grey mould fruit rot of strawberry, and can be by improving radix paeoniae alba extraction concentration or compositely can reaching the purpose of controlling better grey mould fruit rot of strawberry.
The stripped control efficiency of table 5 radix paeoniae alba extraction of the present invention to the strawberry fruit gray mold
Annotate: different lowercases represent significant difference (p<0.05), and different capitalizations represent utmost point significant difference (p<0.01).
Claims (5)
1. the application of radix paeoniae alba extraction in controlling grey mould fruit rot of strawberry.
2. the application of radix paeoniae alba extraction in From Strawberry Leaves gray mold and stripped strawberry fruit gray mold are exsomatized in control.
3. application as claimed in claim 1 or 2, is characterized in that, described radix paeoniae alba extraction is that the piece root extraction of getting the root of herbaceous peony forms.
4. application as claimed in claim 1 or 2, is characterized in that, described radix paeoniae alba extraction is the acetone extract of the root of herbaceous peony.
5. application as claimed in claim 4, is characterized in that, described radix paeoniae alba extraction is, after getting root of herbaceous peony piece root and shining dry grinding, with the analysis pure acetone, White Peony Root to be carried out to lixiviate, gained filtrate evaporation and concentration and obtaining.
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104041538A (en) * | 2014-05-28 | 2014-09-17 | 赵兰 | Natural fungicide for prevention and control of strawberry black spot and preparation method thereof |
| CN104904762A (en) * | 2015-05-21 | 2015-09-16 | 秦廷廷 | Strawberry gray mold control method |
Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102792977A (en) * | 2011-05-25 | 2012-11-28 | 杨松 | Method for preparing pesticide for preventing and controlling aphids of cherry tomatoes |
| CN102805112A (en) * | 2011-05-30 | 2012-12-05 | 王光达 | Botanical pesticide for controlling cucumber bacterial angular leaf spot |
-
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Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102792977A (en) * | 2011-05-25 | 2012-11-28 | 杨松 | Method for preparing pesticide for preventing and controlling aphids of cherry tomatoes |
| CN102805112A (en) * | 2011-05-30 | 2012-12-05 | 王光达 | Botanical pesticide for controlling cucumber bacterial angular leaf spot |
Non-Patent Citations (1)
| Title |
|---|
| 于九伶等: ""13 种药用植物粗提物抑菌活性的初步研究"", 《北京农学院学报》 * |
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104041538A (en) * | 2014-05-28 | 2014-09-17 | 赵兰 | Natural fungicide for prevention and control of strawberry black spot and preparation method thereof |
| CN104041538B (en) * | 2014-05-28 | 2015-11-18 | 赵兰 | A kind ofly prevent and treat natural bactericidal agent of strawberry black spot and preparation method thereof |
| CN104904762A (en) * | 2015-05-21 | 2015-09-16 | 秦廷廷 | Strawberry gray mold control method |
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