CN107318614A - A kind of dendrobium candidum seedling cultivation matrix and preparation method thereof - Google Patents
A kind of dendrobium candidum seedling cultivation matrix and preparation method thereof Download PDFInfo
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- CN107318614A CN107318614A CN201710332948.0A CN201710332948A CN107318614A CN 107318614 A CN107318614 A CN 107318614A CN 201710332948 A CN201710332948 A CN 201710332948A CN 107318614 A CN107318614 A CN 107318614A
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G24/00—Growth substrates; Culture media; Apparatus or methods therefor
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G24/00—Growth substrates; Culture media; Apparatus or methods therefor
- A01G24/20—Growth substrates; Culture media; Apparatus or methods therefor based on or containing natural organic material
- A01G24/22—Growth substrates; Culture media; Apparatus or methods therefor based on or containing natural organic material containing plant material
- A01G24/23—Wood, e.g. wood chips or sawdust
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G24/00—Growth substrates; Culture media; Apparatus or methods therefor
- A01G24/30—Growth substrates; Culture media; Apparatus or methods therefor based on or containing synthetic organic compounds
- A01G24/35—Growth substrates; Culture media; Apparatus or methods therefor based on or containing synthetic organic compounds containing water-absorbing polymers
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Abstract
A kind of dendrobium candidum seedling cultivation matrix and preparation method thereof.The cultivation matrix counts raw material by weight including following：40 50 parts of Cortex Cunninghamiae Lanceolatae, 10 24 parts of peanut skin, 15 30 parts of liver moss, 8 18 parts of fine sand, 12 19 parts of vermiculite, 25 parts of high hydroscopic resin.Preparation method：Weigh raw material；After Cortex Cunninghamiae Lanceolatae, peanut skin, liver moss, vermiculite, fine sand are well mixed, after then clear water is rinsed well in immersion ethanol solution, be placed in plant sterilization mixed liquor soak after it is standby, the plant sterilization mixed liquor is mixed by extract solution from aloe and crocus sativus；Dendrobium candidum seedling cultivation matrix is obtained through treated mixture and high inhale after mixed with resin irradiates 13 seconds through plasma.Cultivation matrix of the present invention is with low cost, and preparation method is simple, can effectively protect seedling by what pure plant sterilized mixed liquor, it is to avoid infection insect pest, and the function such as matrix water-retaining property, the nutrient preserving capability after plasma treatment with irradiation is good.
Description
Technical field
The invention belongs to plant tissue culture field, and in particular to a kind of dendrobium candidum seedling cultivation matrix and preparation method thereof.
Background technology
Dendrobium candidum belongs to Precious, Rare, Endangered high added value medicinal material, and its health care therapeutic effect is definite,《Taoist Scriptures》、《The legendary god of farming
Book on Chinese herbal medicine warp》、《Compendium of Materia Medica》、《Modern Chinese herbal medicine voluminous dictionary》, 2010 years versions《Pharmacopoeia of People's Republic of China》Deng in medical ancient books and records
It is on the books.Tang Kai first year Taoist schools classical works《Taoist Scriptures》Include the big celestial grass of China nine (dendrobium candidum, Herba Saussureae Involueratae, three liang
Weight ginseng, 120 years tuber of multiflower knotweed, the Poria cocos of cycle of sixty years, desert cistanche, remote mountains ganoderma lucidum, seabed pearl, cordyceps sinensis), because of dendrobium candidum
Enriching yin qi-restoratives effect significantly, is listed in the first place of " Chinese nine big celestial grass ".Li Shizhen (1518-1593 A.D.)《Compendium of Materia Medica》Record：" strong the moon strengthening the essence.
Never sufficient, flat stomach gas in long term usage, thick stomach, benefit, long muscle, by skin heat symptoms caused by an exopathgen miliaria gas, the cold numbness of pin knee pain is weak, determines intelligence except shying, gently
Body macrobiosis.QI invigorating is good for sun except heat, by skin wandering arthritis, cold long in bone, kidney tonifying benefit power.Control in heating spontaneous perspiration, ulcer apocenosis and fill in.”
In version in 2010《Pharmacopoeia of People's Republic of China》It is single-row in one, with other dendrobium loddigesii Rolfes, stem of Eyeshaped Dendrobium, HERBA DENDROBII,
The common stem of noble dendrobium species such as HERBA DENDROBII and Dendrobium Chrysotoxum Lindl is distinguished, and its function is with curing mainly：" reinforcing stomach reg fluid, nourishing Yin and clearing heat.For the moon
Hinder body fluid deficiency, dry polydipsia, deficiency of food is retched, after being ill abnormal heat, mesh is secretly failed to understand.”
The stem of noble dendrobium is as a kind of parts of generic medicinal plants, and market demand is larger, and growth cycle is long, although by callus tissue culture, carries
The high growing power of stem of noble dendrobium seedling, but the breeding coefficient of Dendrobium Sw is low, annual production is limited, and into abounding with, time phase is long,
Scale can not be formed, yield is extremely limited, therefore artificial cultivation technique still needs raising.
Existing dendrobium candidum is more based on Cortex Cunninghamiae Lanceolatae or pine bark, is doped with the rubble or haydite of supporting role as cultivation
Matrix is trained, but the gap of rubble or haydite doping is big, is easily fallen into after Cortex Cunninghamiae Lanceolatae or pine bark water suction in gap, is pressed in root system
On, do not simply fail to that tender seedling bar is made, be also unfavorable for root growth, be especially detrimental to the growth of seedlings root.
Publication No. CN103704150A Chinese patent discloses a kind of officinal dendrobium stem plantation dedicated substrate and its application, profit
Cortex Cunninghamiae Lanceolatae discarded object in being processed with China fir, is aided with cork wood particle and small crushed stone, prepares officinal dendrobium stem plantation dedicated substrate, although should
Matrix is loose, the function admirable such as gas permeability, nutrient preserving capability, water-retaining property, with low cost, but the bactericidal ability of the matrix,
It is unfavorable for protecting tissue-cultured seedling.
The content of the invention
In view of the shortcomings of the prior art, it is an object of the invention to provide a kind of dendrobium candidum seedling cultivation matrix and its system
Preparation Method, the cultivation matrix is nutritious, and ventilation moisture retention is good, is conducive to the growth of seedling coring, in addition, preparation method is simple
Single, raw material sources are extensive, with good market prospects.
To solve prior art problem, the technical scheme that the present invention takes is：
A kind of dendrobium candidum seedling cultivation matrix, including following raw material is counted by weight：40-50 parts of Cortex Cunninghamiae Lanceolatae, peanut skin 10-
24 parts, 15-30 parts of liver moss, 8-18 parts of fine sand, 12-19 parts of vermiculite, 2-5 parts of high hydroscopic resin.
It is as improved, above-mentioned dendrobium candidum seedling cultivation matrix, including following counts raw material by weight：Cortex Cunninghamiae Lanceolatae
45 parts, 12 parts of peanut skin, 23 parts of liver moss, 8 parts of fine sand, 15 parts of vermiculite, 3 parts of high hydroscopic resin.
It is that the high hydroscopic resin is starch grafted acrylate polymerization crosslinking thing as improved.
The preparation method of above-mentioned dendrobium candidum seedling cultivation matrix, comprises the following steps：
Step 1, raw material is weighed as required；
Step 2, after Cortex Cunninghamiae Lanceolatae, peanut skin, liver moss, vermiculite, fine sand being well mixed, immersion volume parts are molten for 75% ethanol
10-20 minutes in liquid, after then clear water is rinsed well, be placed in plant sterilization mixed liquor soak 24-40 hours after it is standby, it is described
Plant sterilization mixed liquor is 3-5 according to volume ratio by extract solution from aloe and crocus sativus：1 mixes；
Step 3, dendrobium candidum children is obtained after mixed with resin is irradiated 1-3 seconds through plasma through the treated mixture of step 2 and high inhale
Seedling cultivation matrix.
Be as improved, plant sterilization mixed liquor described in step 2 by extract solution from aloe and crocus sativus according to
Volume ratio is 5：1 mixes.
It is that the frequency of the medium ion exposure of step 3 is 1500HZ as improved.
Beneficial effect
Dendrobium candidum seedling cultivation matrix Component Source of the present invention is extensively, with low cost, and preparation method is simple, is killed by pure plant
Bacterium mixed liquor can effectively protect seedling, it is to avoid infection insect pest, and matrix water-retaining property after plasma treatment with irradiation, protect
The functions such as fertilizer are good, are especially suitable for the growth of candidum tissue culturing seedling.
Embodiment
Embodiment 1
A kind of dendrobium candidum seedling cultivation matrix, including following raw material is counted by weight：40 parts of Cortex Cunninghamiae Lanceolatae, 10 parts of peanut skin,
15 parts of liver moss, 8 parts of fine sand, 12 parts of vermiculite, 2 parts of high hydroscopic resin.
The high hydroscopic resin is starch grafted acrylate polymerization crosslinking thing.
The preparation method of above-mentioned dendrobium candidum seedling cultivation matrix, comprises the following steps：
Step 1, raw material is weighed as required；
Step 2, after Cortex Cunninghamiae Lanceolatae, peanut skin, liver moss, vermiculite, fine sand being well mixed, immersion volume parts are molten for 75% ethanol
10 minutes in liquid, after then clear water is rinsed well, be placed in plant sterilization mixed liquor soak 24 hours after it is standby, the plant kills
Bacterium mixed liquor is 3 according to volume ratio by extract solution from aloe and crocus sativus：1 mixes；
Step 3, after the mixture and high suction mixed with resin treated through step 2 irradiates 1 second through frequency for 1500HZ plasma
Obtain dendrobium candidum seedling cultivation matrix.
Embodiment 2
A kind of dendrobium candidum seedling cultivation matrix, including following raw material is counted by weight：45 parts of Cortex Cunninghamiae Lanceolatae, 12 parts of peanut skin,
23 parts of liver moss, 8 parts of fine sand, 15 parts of vermiculite, 3 parts of high hydroscopic resin.
The high hydroscopic resin is starch grafted acrylate polymerization crosslinking thing.
The preparation method of above-mentioned dendrobium candidum seedling cultivation matrix, comprises the following steps：
Step 1, raw material is weighed as required；
Step 2, after Cortex Cunninghamiae Lanceolatae, peanut skin, liver moss, vermiculite, fine sand being well mixed, immersion volume parts are molten for 75% ethanol
15 minutes in liquid, after then clear water is rinsed well, be placed in plant sterilization mixed liquor soak 30 hours after it is standby, the plant kills
Bacterium mixed liquor is 4 according to volume ratio by extract solution from aloe and crocus sativus：1 mixes；
Step 3, after the mixture and high suction mixed with resin treated through step 2 irradiates 2 seconds through frequency for 1500HZ plasma
Obtain dendrobium candidum seedling cultivation matrix.
Embodiment 3
A kind of dendrobium candidum seedling cultivation matrix, including following raw material is counted by weight：40-50 parts of Cortex Cunninghamiae Lanceolatae, peanut skin 10-
24 parts, 15-30 parts of liver moss, 8-18 parts of fine sand, 12-19 parts of vermiculite, 2-5 parts of high hydroscopic resin.
The high hydroscopic resin is starch grafted acrylate polymerization crosslinking thing.
The preparation method of above-mentioned dendrobium candidum seedling cultivation matrix, comprises the following steps：
Step 1, raw material is weighed as required；
Step 2, after Cortex Cunninghamiae Lanceolatae, peanut skin, liver moss, vermiculite, fine sand being well mixed, immersion volume parts are molten for 75% ethanol
20 minutes in liquid, after then clear water is rinsed well, be placed in plant sterilization mixed liquor soak 40 hours after it is standby, the plant kills
Bacterium mixed liquor is 5 according to volume ratio by extract solution from aloe and crocus sativus：1 mixes；
Step 3, after the mixture and high suction mixed with resin treated through step 2 irradiates 3 seconds through frequency for 1500HZ plasma
Obtain dendrobium candidum seedling cultivation matrix.
Comparative example 1
A kind of dendrobium candidum seedling cultivation matrix, including following raw material is counted by weight：45 parts of Cortex Cunninghamiae Lanceolatae, 12 parts of peanut skin,
23 parts of liver moss, 8 parts of fine sand, 15 parts of vermiculite, 3 parts of high hydroscopic resin.
The high hydroscopic resin is starch grafted acrylate polymerization crosslinking thing.
The preparation method of above-mentioned dendrobium candidum seedling cultivation matrix, comprises the following steps：
Step 1, raw material is weighed as required；
Step 2, after Cortex Cunninghamiae Lanceolatae, peanut skin, liver moss, vermiculite, fine sand being well mixed, immersion volume parts are molten for 75% ethanol
15 minutes in liquid, after then clear water is rinsed well, be placed in plant sterilization mixed liquor soak 30 hours after it is standby, the plant kills
Bacterium mixed liquor is 4 according to volume ratio by extract solution from aloe and crocus sativus：1 mixes；
Step 3, after the mixture and high suction mixed with resin treated through step 2 irradiates 2 seconds through frequency for 1500HZ plasma
Obtain dendrobium candidum seedling cultivation matrix.
Performance test
Matrix to embodiment 1-3 and comparative example 1 carries out water-retaining property detection, and detection method is carried out according to national standard.In addition, with implement
Example 1-3 and the cultivation matrix of comparative example 1 plant dendrobium candidum seedling under same local identical conditions, and iron sheet is detected after 2 months
The upgrowth situation of the stem of noble dendrobium.All experimental datas are as shown in the table.
It is seen from the above data that the upgrowth situation of 1-3 of embodiment of the present invention dendrobium candidum seedling is understood, present invention cultivation
Matrix nutrient preserving capability is good, and water holding capacity is strong, under the same terms, after the cultivation of 2 months, average root a length of 3.8, and average root is thick
0.15cm, average plant height 41cm, and be difficult with the seedling of substrate culture of the present invention by external environmental interference, it is difficult to be invaded by fungi
Dye, improves survival rate.
Claims (6)
1. a kind of dendrobium candidum seedling cultivation matrix, it is characterised in that count raw material by weight including following：Cortex Cunninghamiae Lanceolatae 40-
50 parts, 10-24 parts of peanut skin, 15-30 parts of liver moss, 8-18 parts of fine sand, 12-19 parts of vermiculite, 2-5 parts of high hydroscopic resin.
2. a kind of dendrobium candidum seedling cultivation matrix according to claim 1, it is characterised in that including it is following by weight
Number meter raw material：45 parts of Cortex Cunninghamiae Lanceolatae, 12 parts of peanut skin, 23 parts of liver moss, 8 parts of fine sand, 15 parts of vermiculite, 3 parts of high hydroscopic resin.
3. a kind of dendrobium candidum seedling cultivation matrix according to claim 1, it is characterised in that the high hydroscopic resin is
Starch grafted acrylate polymerization crosslinking thing.
4. the preparation method based on a kind of dendrobium candidum seedling cultivation matrix described in claim 1, it is characterised in that including with
Lower step：
Step 1, raw material is weighed as required；
Step 2, after Cortex Cunninghamiae Lanceolatae, peanut skin, liver moss, vermiculite, fine sand being well mixed, immersion volume parts are molten for 75% ethanol
10-20 minutes in liquid, after then clear water is rinsed well, be placed in plant sterilization mixed liquor soak 24-40 hours after it is standby, it is described
Plant sterilization mixed liquor is 3-5 according to volume ratio by extract solution from aloe and crocus sativus：1 mixes；
Step 3, dendrobium candidum children is obtained after mixed with resin is irradiated 1-3 seconds through plasma through the treated mixture of step 2 and high inhale
Seedling cultivation matrix.
5. a kind of preparation method of dendrobium candidum seedling cultivation matrix according to claim 4, it is characterised in that step 2
Described in plant sterilization mixed liquor by extract solution from aloe and crocus sativus be 5 according to volume ratio：1 mixes.
6. a kind of preparation method of dendrobium candidum seedling cultivation matrix according to claim 4, it is characterised in that step 3
The frequency of medium ion exposure is 1500HZ.
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108934917A (en) * | 2018-06-29 | 2018-12-07 | 贵州健神农业科技发展有限公司 | A kind of dendrobium candidum plantation substrate |
| CN109006343A (en) * | 2018-06-14 | 2018-12-18 | 安徽省霍山县农伯乐开发有限公司 | A kind of preparation method for the planting matrix promoting dendrobium nobile growth quality |
| CN109042209A (en) * | 2018-05-05 | 2018-12-21 | 霍山可胜生态农业专业合作社 | A kind of implantation methods of dendrobium nobile high yield |
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Application publication date: 20171107 |