CN107771675A - A kind of Momordica grosvenori implantation methods - Google Patents

Abstract

The present invention relates to Momordica grosvenori planting technology field, more particularly to a kind of Momordica grosvenori implantation methods, the present invention using tissue culture method first to carrying out nursery, the uniformity of Momordica grosvenori seedling early growth can be effectively improved, ensures advantage seedling, is laid a good foundation for Momordica grosvenori standardized production, tissue culture process rises treatment fluid using disinfectant I and disinfectant II replacement for mercury, sterilizing effect is effectively played, Hg residuals and human injury will not be also caused, be a kind of safe and effective plant disinfectant；Tissue culture process is according to Momordica grosvenori explant, the growth characteristics of seedling different phase, the growth of Momordica grosvenori explant is stimulated using alternate illumination method, MS culture mediums are improved specific have configured initial medium, inducing culture, subculture medium, root media, promote explant physical efficiency effectively to carry out cell differentiation, improve the survival rate of tissue-cultured seedling；Decomposed in advance, the survival rate of raising Momordica grosvenori seedling cultivation is carried out to plantation plot.

Description

A kind of Momordica grosvenori implantation methods

【Technical field】

The present invention relates to Momordica grosvenori planting technology field, more particularly to a kind of Momordica grosvenori implantation methods.

【Background technology】

Momordica grosvenori (scientific name：Siraitia grosvenorii) belong to Curcurbitaceae herbaceous perennial vine plant, its fruit is The famous Chinese medicine in China and traditional exporting, people is existing to the tissue culture technique of Momordica grosvenori at present has goed deep into compared with system Research, in recent years, be engaged in the enterprise of Momordica grosvenori industrial seedling rearing up to more than ten families, be distributed in Guilin, Nanning, Liuzhou and Wuhan Etc. ground, for tissue-cultured seedling cultivated area up to more than 30,000 mus, Luohanguo With Plantlets of Tissue Culture shows breeding relative to the keep down the vines of a creeping plant potato seedling growth of breeding of tradition Coefficient is high, does not carry the advantages such as virus, growth potential are strong, adaptability is wider, advances the development of Momordica grosvenori planting industry.But mesh The nursery stock of preceding different company or batch causes not there is planting survival rates, the unstability for strain rate etc. of yielding positive results With the economic loss of degree, Planting risk is added, and harmful effect is generated to whole Momordica grosvenori industry sustainable development.

Moreover, currently used tissue culture medium (TCM) selection is MS culture mediums, culture medium raw material composition is single, and survival rate is not It is stable.The conventional antibacterial disinfectant in China mainly based on mercuric chloride, has carried out substantial amounts of research to the toxic action of mercury both at home and abroad, Confirm that mercury all has a major impact to each growth course of cell, so the explant to be sterilized by mercuric chloride, need to thoroughly be removed residual The Hg+ stayed, to eliminate injury of the micro residual mercuric chloride to explant.Therefore, in order to improve Luohanguo With Plantlets of Tissue Culture survival rate, make Momordica grosvenori seedling can be standardized, the production that standardizes is necessary to be improved existing tissue culture, planting technology, search out A kind of method for adapting to Momordica grosvenori growth of seedling, improve the survival rate of Momordica grosvenori.

【The content of the invention】

In view of the above, it is necessary to be improved to existing tissue culture, planting technology, search out one kind and adapt to arhat The method of fruit growth of seedling, improve the survival rate of Momordica grosvenori.

To reach above-mentioned purpose, the technical solution adopted in the present invention is：

A kind of Momordica grosvenori implantation methods, methods described include nursery and plantation step：

(1) nursery：

(1) explant is obtained：Choose without disease pest harm, the Momordica grosvenori spray clip explant buddingged, before explant clip First scissors mouth is carried out disinfection to spray spray disinfectant I, and with the ethanol solution that percentage by volume is 75%-85%；Will be tender Stem section is cut into the segment for being 2cm-4cm with axillary bud, length with scissors after branch removal blade, and segment is put into temperature as -2 DEG C Refrigerated in~0 DEG C of frozen water；

(2) sterilization of explant：Explant after step (1) refrigeration 20min-40min is put into percentage by volume is Soaked in 75% ethanol solution, be put into after draining in thimerosal II and soak 40min-60min, complete disinfecting process；

(3) it is inoculated with：The explant oblique cutting that step (2) disinfects is entered on initial medium, axillary bud upward, then will culture Base is put into darkroom to be cultivated under conditions of temperature is 24 DEG C -26 DEG C；

(4) Fiber differentiation：After the explant of step (3) grows sterile bud, clip length is 3cm-5cm band bud explant The cuttage of body top is cultivated under conditions of being 24 DEG C -26 DEG C in temperature on inducing culture, explant is given daily during culture Body carries out photo-irradiation treatment；

(5) squamous subculture：When the explant of step (4) grow to height be 5cm-7cm when obtain seedling, by seedling go to after For in culture medium, it is 24 DEG C -26 DEG C in temperature and is cultivated, photo-irradiation treatment, light are carried out to seedling with incandescent lamp daily during supporting It is daily 4h-6h, intensity of illumination 700Lx-900Lx according to processing time；

(6) culture of rootage：When the seedling of step (5) grows the root system of 2cm-3cm length, seedling is gently extracted to subculture training Base is supported, and the subculture medium residuals for being attached to root are washed with clear water, then inserts seedling in root media Cultivated, and move into culturing rack and cultivated, daytime, cultivation temperature was 22 DEG C -30 DEG C, and night cultivation temperature is 15 DEG C -20 DEG C and seedling in culturing rack is irradiated using incandescent lamp, a length of daily 8h-12h, intensity of illumination 400Lx- during irradiation 600Lx；

(7) hardening：Step (6) is highly put into greenhouse for 10cm-13cm seedling and carries out hardening, hardening temperature is 22 DEG C -30 DEG C, illumination is solar radiation, relative humidity 10%-20%；

(2) plant：After hardening 50d-60d, the selection healthy seedling that cane is sturdy, leaf color is dark green is in decomposed plantation plot On planted according to 20cm-30cm × 20cm-30cm seeding row spacing, planting process irregularly spray expelling parasite water keep planting site The relative humidity of block is 10%-20%, until Momordica grosvenori harvests.

Further, step (1) the thimerosal I：By 10g-15g barbaloin, 2g-5g sodium chloride, 10g-20g After the mixing of Radix et Caulis Opuntiae Dillenii extract and 8g-15g Lotus Leafextract, the ethanol solution that percentage by volume is 75% is added, and will Solution is settled to 1000mL and is made.

Further, step (2) the thimerosal II is by 20g-25g shrubby sophora extract, 2g-5g sodium hydroxide, 2g- After the willow bark extract mixing of 5g sodium acid carbonate, 20g-25g triterpene glucoside and 20g-25g, volume basis is added Number is 75% ethanol solution, and solution is settled into 1000mL and is made.

Further, step (3) initial medium is the MS culture mediums of improvement：1mg/L-2mg/L 6- benzyl glands Purine, 3mg/L-5mg/L aloe polysaccharide, 2mg/L-4mg/L chitosan, 3mg/L-6mg/L extract of soybean, 4g/L- 6g/L agar, the ethanol that 20mL-40mL percentage by volumes are 75%, remaining part are MS culture mediums, pH 5-6.

Further, step (4) inducing culture is the MS culture mediums of improvement：1mg/L-2mg/L methyl α-naphthyl acetate, 2mg/L-4mg/L gumbo polysaccharide, 2mg/L-4mg/L cactus polyoses, 3mg/L-6mg/L peanut extract, 5mg/L- 8mg/L soybean meal extractive, 4g/L-6g/L agar, 30mL-50mL percentage by volumes are 75% ethanol, and remaining part is MS culture mediums, pH 5-6.

Further, the processing method of step (4) the explant progress photo-irradiation treatment is：With white light, blue light, feux rouges, Gold-tinted carries out circulation light photograph, and the order that the circulation light shines is " white light 30min- blue light 20min- feux rouges 30min- gold-tinteds 30min- white light 30min " illumination total durations are 6h-9h, and the intensity of illumination of the white light is 300Lx-400Lx, the illumination of blue light Intensity is 400Lx-500Lx, and the intensity of illumination of feux rouges is 500Lx-600Lx, and the intensity of illumination of gold-tinted is 300Lx-400Lx.

Further, step (5) subculture medium is the MS culture mediums of improvement：1mg/L-2mg/L heteroauxin, 2mg/L-4mg/L chitosan, 2mg/L-4mg/L silkworm excrement soaking water, 3mg/L-6mg/L oily tealeaf extract, 3mg/L- 6mg/L white mulberry vein-juice, 5mg/L-8mg/L activated carbon, 4g/L-6g/L agar, 20mL-40mL percentage by volumes are 75% Ethanol, remaining part are MS culture mediums, pH 5-6.

Further, step (6) root media is the MS culture mediums of improvement：1mg/L-2mg/L heteroauxin, 1mg/L-2mg/L methyl α-naphthyl acetate, 2mg/L-4mg/L chitosan, 2mg/L-4mg/L silkworm excrement soaking water, 2mg/L-4mg/L flowers Raw shell extract, 3mg/L-6mg/L oily tealeaf extract, 3mg/L-6mg/L polygonum flaccidum grass juice factor, 10mg/L-20mg/L work Property charcoal, 4g/L-6g/L agar, 20mL-40mL percentage by volumes be 75% ethanol, remaining part is MS culture mediums, and pH is 5-6。

Further, the decomposed method in the plantation plot is：By fresh white flower pea, fresh alfalfa, fresh Trifolium repense Grass, rice straw, oil tea slag and fresh Chinese milk vetch are 1-3 according to mass ratio:1-3:2-4:2-4:3-5:1-3 is mixed, and is then pressed According to 100mg/g-150mg/g addition sprayed into mixture enzyme activity unit be 900U/g-1200U/g cellulase and Enzyme activity unit is the mixed liquor of 800U/g-1000U/g pectase, and the mass ratio of the cellulase and pectase is：1: 1-2；Enzymolysis mixture is uniformly shed in plot is planted in breeding after enzymolysis 48h-52h, and it is 2cm-3cm to cover last layer thickness Soil carry out decomposed, decomposed total duration be 40d-45d, and decomposed temperature is 22 DEG C -27 DEG C.

Further, the expelling parasite water comprises the following components in parts by weight：10 parts -15 parts of Radix Sophorae Flavescentis extractive liquid, 5 parts - 10 parts of lucidum extracting liquid, 5 parts -10 parts of eucalyptus extract solution, 7 parts -19 parts of Radix Astragali extractive solution, 35 parts -45 parts of volume hundred Ethanol solution, 30 part -40 part of water of the fraction for 75%.

The present invention has the advantages that：

The present invention carries out nursery using tissue culture method, can effectively improve the uniformity of Momordica grosvenori seedling early growth, ensures excellent Gesture seedling, laid a good foundation for Momordica grosvenori standardized production, disinfectant I and disinfectant II replacement for mercury are utilized in tissue culture procedures Treatment fluid is risen, sterilizing can be effectively played and acted on, disinfectant I and disinfectant II composition are mostly plant components, will not be caused Hg is remained and human injury, is a kind of safe and effective plant disinfectant；Spy of the present invention always according to Momordica grosvenori growth of seedling Property, stimulate Momordica grosvenori explant to grow with alternate illumination method, induction Momordica grosvenori explant is taken root；Invention is according to Momordica grosvenori explant Body, seedling different times are different to the demand of nutriment, and MS culture mediums are improved, specific to have configured initial training Base, inducing culture, subculture medium, root media are supported, it is effective that these culture mediums can effectively promote explant physical efficiency to carry out Cell differentiation, promote the growth of tissue-cultured seedling, improve the survival rate of tissue-cultured seedling；Apply the characteristic always according to tissue-cultured seedling to planting plot Progress is decomposed in advance, effectively increases the survival rate of Momordica grosvenori seedling cultivation.

【Embodiment】

All features disclosed in this specification, or disclosed all methods or during the step of, except mutually exclusive Feature and/or step beyond, can combine in any way.

Any feature disclosed in this specification (including any accessory claim, summary), unless specifically stated otherwise, each Feature is an example in a series of equivalent or similar characteristics.

Embodiment 1：

A kind of Momordica grosvenori implantation methods are present embodiments provided, this method includes nursery and plantation step：

(1) nursery：

(1) explant is obtained：Choose without disease pest harm, the Momordica grosvenori spray clip explant buddingged, before explant clip First to spray spray disinfectant I, and scissors mouth is carried out disinfection with the ethanol solution that percentage by volume is 75%；Spray is removed Stem section is cut into the segment for being 2cm with axillary bud, length with scissors after blade, and segment is put into the frozen water that temperature is -2 DEG C Row refrigeration；

(2) sterilization of explant：Step (1) is refrigerated to the explant after 20min and is put into the second that percentage by volume is 75% Soaked in alcoholic solution, be put into after draining in thimerosal II and soak 40min, complete disinfecting process；

(3) it is inoculated with：The explant oblique cutting that step (2) disinfects is entered on initial medium, axillary bud upward, then will culture Base is put into darkroom to be cultivated under conditions of temperature is 24 DEG C；

(4) Fiber differentiation：After the explant of step (3) grows sterile bud, clip length is 3cm band bud explant top Cuttage is held on inducing culture, temperature be 24 DEG C-under the conditions of cultivated, give explant to carry out illumination daily during culture Processing；Wherein, the processing method of explant progress photo-irradiation treatment is：Circulation light photograph, institute are carried out with white light, blue light, feux rouges, gold-tinted The order for stating circulation light photograph is " when white light 30min- blue light 20min- feux rouges 30min- gold-tinted 30min- white light 30min " illumination is total A length of 6h, the intensity of illumination of the white light is 300Lx, and the intensity of illumination of blue light is 400Lx, and the intensity of illumination of feux rouges is 500Lx, The intensity of illumination of gold-tinted is 300Lx.

(5) squamous subculture：Grown to when the explant of step (4) when height is 5cm and obtain seedling, seedling is gone into subculture training Support in base, being 24 DEG C in temperature is cultivated, and photo-irradiation treatment, photo-irradiation treatment time are carried out to seedling with incandescent lamp daily during supporting For daily 4h, intensity of illumination 700Lx；

(6) culture of rootage：When the seedling of step (5) grows the root system of 2cm length, seedling is gently extracted into squamous subculture Base, and the subculture medium residuals for being attached to root are washed with clear water, then seedling is inserted in root media Row culture, and move into culturing rack and cultivated, daytime, cultivation temperature was 22 DEG C, and night cultivation temperature is 15 DEG C and using incandescent Lamp is irradiated to seedling in culturing rack, a length of daily 8h, intensity of illumination 400Lx during irradiation；

(7) hardening：Step (6) is highly put into greenhouse for 10cm seedling and carries out hardening, hardening temperature is 22 DEG C, light According to for solar radiation, relative humidity 10%；

(2) plant：

After hardening 50d, selection cane is sturdy, leaf color is dark green healthy seedling on decomposed plantation plot according to 20cm × 20cm seeding row spacing is planted, and it is 10% that planting process, which irregularly sprays expelling parasite water to keep the relative humidity in plantation plot, directly Harvested to Momordica grosvenori.Wherein, the decomposed method for planting plot is：By fresh white flower pea, fresh alfalfa, fresh butch clover, Rice straw, oil tea slag and fresh Chinese milk vetch are 1 according to mass ratio:1:2:2:3:1 is mixed, then according to 100mg/g addition Measure and the mixed of the cellulase that enzyme activity unit is 900U/g and the pectase that enzyme activity unit is 800U/g is sprayed into mixture Liquid is closed, the mass ratio of the cellulase and pectase is：1:1；Enzymolysis mixture is uniformly shed after enzymolysis 48h and planted in breeding In plot, and cover the soil that last layer thickness is 2cm and carry out decomposed, decomposed total duration be 40d, and decomposed temperature is 22 DEG C.

Each thimerosal of the present embodiment, culture medium prescription are as follows：

Thimerosal I：

After 10g barbaloin, 2g sodium chloride, 10g Radix et Caulis Opuntiae Dillenii extract and 8g Lotus Leafextract mixing, then add Enter percentage by volume and be 75% ethanol solution, and solution is settled to 1000mL and is made.

The extracting method of Radix et Caulis Opuntiae Dillenii extract is in the present embodiment thimerosal I：Fresh cactus is taken into thorn, smashed to pieces, then According to solid-liquid mass ratio it is 1 with the ethanol solution that percentage by volume is 75%:2 are mixed, and mixture then is put into -20 DEG C Refrigerator-freezer in suddenly freeze 10h, then mixture is placed in supersonic extractors and carries out ultrasonic extraction, Extracting temperature is 50 DEG C, during extraction Between be 40min, extraction power is 700w, mixture is put into 120 DEG C of refluxing extraction device again carries out refluxing extraction afterwards 12h, filter off except filter residue, filtrate is subjected to rotary evaporation concentration, drying until moisture content obtains Radix et Caulis Opuntiae Dillenii extract for 5%； The content of cactus polyoses is 102.36mg/g in extract；General flavone content is 187.03mg/g.

The extracting method of Lotus Leafextract is in the present embodiment thimerosal I：The lotus leaf for taking moisture content to be 5% shreds, then It is 1 according to solid-liquid mass ratio:1 adds the ethanol solution that percentage by volume is 75%, is put into after being mixed in refluxing extraction device Refluxing extraction is carried out, Extracting temperature is 150 DEG C, extraction time 10h, is filtered off except filter residue, and it is dense that filtrate is carried out into rotary evaporation Contracting, dry until moisture content obtains Lotus Leafextract for 5%；The content of Nuciferine is 102.31mg/g in extract.

Thimerosal II：

By 20g shrubby sophora extract, 2g sodium hydroxide, 2g sodium acid carbonate, 20g triterpene glucoside and 20g poplar After the mixing of willow bark extract, add percentage by volume and be 75% ethanol solution, and solution is settled to 1000mL and is made.

The extracting method of shrubby sophora extract is in the present embodiment thimerosal II：The kuh-seng dry plate that moisture content is 5% is taken, then It is 1 according to solid-liquid mass ratio:2 add the ethanol solution that percentage by volume is 75%, are put into after being mixed in refluxing extraction device Refluxing extraction is carried out, Extracting temperature is 180 DEG C, extraction time 12h, is filtered off except filter residue, and it is dense that filtrate is carried out into rotary evaporation Contracting, dry until moisture content obtains shrubby sophora extract for 5%；The content of matrine is 113.25mg/g in extract, and flavones contains Measure as 85.21mg/g.

The extracting method of willow bark extract is in the present embodiment thimerosal II：The willow piece that moisture content is 5% is taken, so It is afterwards 1 according to mass ratio by willow bark, 75% ethanol solution, sodium hydroxide:2：3 are mixed, and are then placed in backflow and are carried Take and refluxing extraction is carried out in device, Extracting temperature is 170 DEG C, extraction time 10h, filters off except filter residue, filtrate is rotated It is concentrated by evaporation, dries until moisture content obtains willow extract for 5%；The content of sodium salicylate is 251.1mg/g in extract, Bigcatkin willow glucoside is 85.21mg/g.

Initial medium：

It is as follows for the MS culture mediums of improvement, formula：

1mg/L 6-benzyladenine, 3mg/L aloe polysaccharide, 2mg/L chitosan, 3mg/L extract of soybean, 4g/L agar, the ethanol that 20mL percentage by volumes are 75%, remaining part are MS culture mediums, pH 5.

The extracting method of extract of soybean is in the present embodiment initial medium：According to mass ratio it is 1 by soybean and water:1 It is put into after immersion 12h in disintegrating machine and is crushed to obtain mixed liquor, then addition is with mixed liquor mass ratio into mixed liquor again 1:1 percentage by volume is 75% ethanol solution, is afterwards put into mixed liquor and carries out backflow in 130 DEG C of refluxing extraction device and carry 14h is taken, is filtered off except filter residue, filtrate is subjected to rotary evaporation concentration, drying until moisture content obtains extract of soybean for 4%； Daizeol content is 201.25mg/g in extract；The content of isoflavones is 85.64mg/g.

Inducing culture：

It is as follows for the MS culture mediums of improvement, formula：

1mg/L methyl α-naphthyl acetate, 2mg/L gumbo polysaccharide, 2mg/L cactus polyoses, 3mg/L peanut extract, 5mg/L soybean meal extractive, 4g/L agar, 30mL percentage by volumes are 75% ethanol, and remaining part is MS culture mediums, pH For 5-6.

The extracting method of peanut extract is in the present embodiment inducing culture：According to mass ratio it is 1 by peanut and water:2 It is put into after immersion 12h in disintegrating machine and is crushed to obtain mixed liquor, then addition is with mixed liquor mass ratio into mixed liquor again 1:1 percentage by volume is 75% ethanol solution, is afterwards put into mixed liquor and carries out backflow in 140 DEG C of refluxing extraction device and carry 14h is taken, is filtered off except filter residue, filtrate is subjected to rotary evaporation concentration, drying until moisture content obtains extract of soybean for 4%； Extract Linoleic acid content is 135.25mg/g；The content of cholesterol is 107.15mg/g.

The extracting method of soybean meal extractive is in the present embodiment inducing culture：It is 75% by dregs of beans and percentage by volume Ethanol solution is 1 according to mass ratio:3 are mixed, and are then put into mixture and are carried out backflow in 130 DEG C of refluxing extraction device and carry 14h is taken, is filtered off except filter residue, filtrate is subjected to rotary evaporation concentration, drying until moisture content obtains soybean meal extractive for 4%； Daizeol content is 184.12mg/g in extract；The content of isoflavones is 96.54mg/g.

Subculture medium：

It is as follows for the MS culture mediums of improvement, formula：

1mg/L heteroauxin, 2mg/L chitosan, 2mg/L silkworm excrement soaking water, 3mg/L oily tealeaf extract, 3mg/L white mulberry vein-juice, 5mg/L activated carbon, 4g/L agar, 20mL percentage by volumes are 75% ethanol, and remaining part is MS culture mediums, pH 5.

The processing method of silkworm excrement soaking water is in the present embodiment subculture medium：The silkworm excrement that moisture content is 5% is pressed with water It is 1 according to solid-liquid mass ratio:3 are mixed, and then add 5g/L active dry yeast, are filtered off after the 16d that ferments except filter residue obtains Silkworm excrement soaking water.

The extracting method of oily tealeaf extract is in the present embodiment subculture medium：The oil tea slag that oil content is 5% is pressed It is 1 according to solid-liquid mass ratio:4 are mixed with the ethanol solution that percentage by volume is 75%, and mixture then is put into 120 DEG C Refluxing extraction 14h is carried out in refluxing extraction device, is filtered off except filter residue, filtrate is subjected to rotary evaporation concentration, drying until aqueous Rate obtains oily tealeaf extract for 4%；Unsaturated fatty acid content is 132.12mg/g in extract；The content of oil tea alkali is 98.54mg/g。

The processing method of white mulberry vein-juice is in the present embodiment subculture medium：It is according to solid-liquid mass ratio with water by new fresh mulberry leaf 2:1 is mixed, and then adds 4g/L cellulase, is filtered off after digesting 24h except filter residue obtains white mulberry vein-juice.

Root media：

It is as follows for the MS culture mediums of improvement, formula：

1mg/L heteroauxin, 1mg/L methyl α-naphthyl acetate, 2mg/L chitosan, 2mg/L silkworm excrement soaking water, 2mg/L flowers Raw shell extract, 3mg/L oily tealeaf extract, 3mg/L polygonum flaccidum grass juice factor, 10mg/L activated carbon, 4g/L agar, 20mL Percentage by volume is 75% ethanol, and remaining part is MS culture mediums, pH 5.

The processing method of the present embodiment root media silkworm excrement soaking water is：By silkworm excrement and water that moisture content is 5% according to Solid-liquid mass ratio is 1:2 are mixed, and then add 5g/L active dry yeast, are filtered off after the 16d that ferments except filter residue obtains silkworm Husky soaking water.

The extracting method of Extracts from Peanut Hulls is in the present embodiment root media：By the peanut shell powder that moisture content is 5% Shine and screen by 60 mesh after broken, be then 1 according to solid-liquid mass ratio:4 are mixed with percentage by volume for 75% ethanol solution Close, then mixture is put into progress refluxing extraction 12h in 150 DEG C of refluxing extraction device, filter off except filter residue, filtrate is carried out Rotary evaporation concentration, dry until moisture content obtains oily tealeaf extract for 4%；General flavone content is in extract 214.05mg/g；The content of alkaloid is 105.31mg/g

The extracting method of oily tealeaf extract is in the present embodiment root media：The oil tea slag that oil content is 5% is pressed It is 1 according to solid-liquid mass ratio:3 are mixed with the ethanol solution that percentage by volume is 75%, and mixture then is put into 130 DEG C Refluxing extraction 12h is carried out in refluxing extraction device, is filtered off except filter residue, filtrate is subjected to rotary evaporation concentration, drying until aqueous Rate obtains oily tealeaf extract for 4%；Unsaturated fatty acid content is 105.33mg/g in extract；The content of oil tea alkali is 78.09mg/g。

The processing method of polygonum flaccidum grass juice factor is in the present embodiment root media：By fresh flaccid knotweed herb and water according to solid-liquid quality Than for 32:1 is mixed, and then adds 8g/L cellulase, is filtered off after digesting 24h except filter residue obtains polygonum flaccidum grass juice factor.

Expelling parasite water：

Comprise the following components in parts by weight：10 parts of Radix Sophorae Flavescentis extractive liquid, 5 parts of lucidum extracting liquid, the extraction of 5 parts of eucalyptus Liquid, 7 parts of Radix Astragali extractive solution, the ethanol solution that 35 parts of percentage by volume is 75%, 30 parts of water.

The extracting method of Radix Sophorae Flavescentis extractive liquid is in the present embodiment expelling parasite water：The fresh whole strain of kuh-seng is smashed to pieces, then according to Solid-liquid mass ratio is 1:2 add the ethanol solution that percentage by volume is 75%, are put into refluxing extraction device and carry out after being mixed Refluxing extraction, Extracting temperature are 180 DEG C, extraction time 12h, are filtered off except filter residue, by the concentration of filtrate progress rotary evaporation, directly To concentrate Radix Sophorae Flavescentis extractive liquid is obtained for the 1/5 of stoste；The content of matrine is 54.21mg/g in extract solution, and flavones content is 23.65mg/g。

The extracting method of lucidum extracting liquid is in the present embodiment expelling parasite water：The ganoderma lucidum that moisture content is 5% is crushed, passed through The screening of 100 eye mesh screens is crossed, is then 1 according to solid-liquid mass ratio:3 add the ethanol solution that percentage by volume is 75%, are mixed It is put into after conjunction in refluxing extraction device and carries out refluxing extraction, Extracting temperature is 120 DEG C, extraction time 12h, is filtered off except filter residue, Filtrate is subjected to rotary evaporation concentration, until concentrate obtains lucidum extracting liquid for the 1/5 of stoste；GL-B in extract solution Content is 84.61mg/g.

The extracting method of eucalyptus extract solution is in the present embodiment expelling parasite water：By moisture content be 5% eucalyptus leaves and press bark It is 1 according to mass ratio:Crush after 33 mixing and screened by 100 eye mesh screens, is then divided into two parts powder, a copy of it is put into 3 Refluxing extraction 13h in times mass parts, the butanol solution that butanol percentage by volume is 20%, filter to take solvent be evaporated under reduced pressure, Dry until moisture content obtains eucalyptus extracts I for 3%；Another is put into 4 times of mass parts, petroleum ether percentage by volume is 10% Petroleum ether solution in refluxing extraction 13h, filter to take solvent and be evaporated under reduced pressure, until concentrate obtains eucalyptus for the 1/5 of stoste Set extract solution.In this implementation eucalyptus extract solution, ter penoidses content is 23.57mg/g, flavones ingredient content is 96.58mg/ G, tannin compositions content is 26.35mg/g, phloroglucinol derivatives component content is 89.64mg/g, glycoside component content is 76.52mg/g。

The extracting method of Radix Astragali extractive solution is in the present embodiment expelling parasite water：The Radix Astragali that moisture content is 5% is crushed, passed through The screening of 80 eye mesh screens is crossed, is then 1 according to solid-liquid mass ratio:4 add the ethanol solution that percentage by volume is 75%, are mixed After be put into refluxing extraction device and carry out refluxing extraction, Extracting temperature is 150 DEG C, extraction time 16h, is filtered off except filter residue, will Filtrate carries out rotary evaporation concentration, until concentrate obtains Radix Astragali extractive solution for the 1/5 of stoste；Bitter taste cellulose content is in extract solution 132.54mg/g, astragaloside content are 146.25mg/g, beet alkali content 63.25mg/g, content of choline 95.16mg/ G, folate content is 64.25mg/g.

Embodiment 2：

A kind of Momordica grosvenori implantation methods are present embodiments provided, this method includes nursery and plantation step：

(1) nursery：

(1) explant is obtained：Choose without disease pest harm, the Momordica grosvenori spray clip explant buddingged, before explant clip First to spray spray disinfectant I, and scissors mouth is carried out disinfection with the ethanol solution that percentage by volume is 85%；Spray is removed Stem section is cut into the segment for being 4cm with axillary bud, length with scissors after blade, and segment is put into the frozen water that temperature is 0 DEG C Row refrigeration；

(2) sterilization of explant：Step (1) is refrigerated to the explant after 40min and is put into the second that percentage by volume is 75% Soaked in alcoholic solution, be put into after draining in thimerosal II and soak 60min, complete disinfecting process；

(3) it is inoculated with：The explant oblique cutting that step (2) disinfects is entered on initial medium, axillary bud upward, then will culture Base is put into darkroom to be cultivated under conditions of temperature is 26 DEG C；

(4) Fiber differentiation：After the explant of step (3) grows sterile bud, clip length is 5cm band bud explant top Cuttage is held to inducing culture, is cultivated under conditions of being 26 DEG C in temperature, light is carried out to explant daily during culture According to processing；Wherein, the processing method of explant progress photo-irradiation treatment is：Circulation light photograph is carried out with white light, blue light, feux rouges, gold-tinted, The order that the circulation light shines is " white light 30min- blue light 20min- feux rouges 30min- gold-tinted 30min- white light 30min " illumination is total Shi Changwei 9h, the intensity of illumination of the white light is 400Lx, and the intensity of illumination of blue light is 500Lx, and the intensity of illumination of feux rouges is 600Lx, the intensity of illumination of gold-tinted is 400Lx.

(5) squamous subculture：Grown to when the explant of step (4) when height is 7cm and obtain seedling, seedling is gone into subculture training Support in base, being 26 DEG C in temperature is cultivated, and photo-irradiation treatment, photo-irradiation treatment time are carried out to seedling with incandescent lamp daily during supporting For daily 6h, intensity of illumination 900Lx；

(6) culture of rootage：When the seedling of step (5) grows the root system of 3cm length, seedling is gently extracted into squamous subculture Base, and the subculture medium residuals for being attached to root are washed with clear water, then seedling is inserted in root media Row culture, and move into culturing rack and cultivated, daytime, cultivation temperature was 30 DEG C, and night cultivation temperature is 20 DEG C and using incandescent Lamp is irradiated to seedling in culturing rack, a length of daily 12h, intensity of illumination 600Lx during irradiation；

(7) hardening：Step (6) is highly put into greenhouse for 13cm seedling and carries out hardening, hardening temperature is 30 DEG C, light According to for solar radiation, relative humidity 20%；

(2) plant：

After hardening 60d, selection cane is sturdy, leaf color is dark green healthy seedling on decomposed plantation plot according to 30cm × 30cm seeding row spacing is planted, and it is 20% that planting process, which irregularly sprays expelling parasite water to keep the relative humidity in plantation plot, directly Harvested to Momordica grosvenori.Wherein, the decomposed method for planting plot is：By fresh white flower pea, fresh alfalfa, fresh butch clover, Rice straw, oil tea slag and fresh Chinese milk vetch are 3 according to mass ratio:3:4:4:5:3 are mixed, then according to 150mg/g addition Measure and the cellulase that enzyme activity unit is 1200U/g and the pectase that enzyme activity unit is 1000U/g are sprayed into mixture Mixed liquor, the mass ratio of the cellulase and pectase are：1:2；Enzymolysis mixture is uniformly shed in breeding after enzymolysis 52h Plant in plot, and cover the soil that last layer thickness is 3cm and carry out decomposed, decomposed total duration be 45d, and decomposed temperature is 27 DEG C.

Each thimerosal of the present embodiment, culture medium prescription are as follows：

Thimerosal I：

After 15g barbaloin, 5g sodium chloride, 20g Radix et Caulis Opuntiae Dillenii extract and 15g Lotus Leafextract mixing, then Add percentage by volume and be 75% ethanol solution, and solution is settled to 1000mL and is made.

The extracting method of Radix et Caulis Opuntiae Dillenii extract and Lotus Leafextract and embodiment 1 are completely the same in the present embodiment thimerosal I.

Thimerosal II：

By 25g shrubby sophora extract, 5g sodium hydroxide, 5g sodium acid carbonate, 25g triterpene glucoside and 25g poplar After the mixing of willow bark extract, add percentage by volume and be 75% ethanol solution, and solution is settled to 1000mL and is made.

The extracting method of shrubby sophora extract and willow bark extract and embodiment 1 complete one in the present embodiment thimerosal II Cause.

Initial medium：

It is as follows for the MS culture mediums of improvement, formula：

2mg/L 6-benzyladenine, 5mg/L aloe polysaccharide, 4mg/L chitosan, 6mg/L extract of soybean, 6g/L agar, the ethanol that 40mL percentage by volumes are 75%, remaining part are MS culture mediums, pH 6.

The extracting method of extract of soybean and embodiment 1 are completely the same in the present embodiment initial medium.

Inducing culture：

It is as follows for the MS culture mediums of improvement, formula：

2mg/L methyl α-naphthyl acetate, 4mg/L gumbo polysaccharide, 4mg/L cactus polyoses, 6mg/L peanut extract, 8mg/L soybean meal extractive, 6g/L agar, 50mL percentage by volumes are 75% ethanol, and remaining part is MS culture mediums, pH For 6.

The extracting method of peanut extract and soybean meal extractive and embodiment 1 complete one in the present embodiment inducing culture Cause.

Subculture medium：

It is as follows for the MS culture mediums of improvement, formula：

2mg/L heteroauxin, 4mg/L chitosan, 4mg/L silkworm excrement soaking water, 6mg/L oily tealeaf extract, 6mg/L white mulberry vein-juice, 8mg/L activated carbon, 6g/L agar, 40mL percentage by volumes are 75% ethanol, and remaining part is MS culture mediums, pH 6.

Silkworm excrement soaking water, oily tealeaf extract, the processing method of white mulberry vein-juice and embodiment 1 in the present embodiment subculture medium It is completely the same.

Root media：

It is as follows for the MS culture mediums of improvement, formula：

2mg/L heteroauxin, 2mg/L methyl α-naphthyl acetate, 4mg/L chitosan, 4mg/L silkworm excrement soaking water, 4mg/L flowers Raw shell extract, 6mg/L oily tealeaf extract, 6mg/L polygonum flaccidum grass juice factor, 20mg/L activated carbon, 6g/L agar, 40mL Percentage by volume is 75% ethanol, and remaining part is MS culture mediums, pH 6.

The processing of the present embodiment root media silkworm excrement soaking water, Extracts from Peanut Hulls, oily tealeaf extract, polygonum flaccidum grass juice factor Method and embodiment 1 are completely the same.

Expelling parasite water：

Comprise the following components in parts by weight：15 parts of Radix Sophorae Flavescentis extractive liquid, 10 parts of lucidum extracting liquid, 10 parts of eucalyptus carry Take liquid, 19 parts of Radix Astragali extractive solution, the ethanol solution that 45 parts of percentage by volume is 75%, 40 parts of water.

Radix Sophorae Flavescentis extractive liquid, lucidum extracting liquid, eucalyptus extract solution, the extracting method of Radix Astragali extractive solution in the present embodiment expelling parasite water It is completely the same with embodiment 1.

Embodiment 3：

A kind of Momordica grosvenori implantation methods are present embodiments provided, this method includes nursery and plantation step：

(1) nursery：

(1) explant is obtained：Choose without disease pest harm, the Momordica grosvenori spray clip explant buddingged, before explant clip First to spray spray disinfectant I, and scissors mouth is carried out disinfection with the ethanol solution that percentage by volume is 80%；Spray is removed Stem section is cut into the segment for being 3cm with axillary bud, length with scissors after blade, and segment is put into the frozen water that temperature is -1 DEG C Row refrigeration；

(2) sterilization of explant：Step (1) is refrigerated to the explant after 30min and is put into the second that percentage by volume is 75% Soaked in alcoholic solution, be put into after draining in thimerosal II and soak 50min, complete disinfecting process；

(3) it is inoculated with：The explant oblique cutting that step (2) disinfects is entered on initial medium, axillary bud upward, then will culture Base is put into darkroom to be cultivated under conditions of temperature is 25 DEG C；

(4) Fiber differentiation：After the explant of step (3) grows sterile bud, clip length is 4cm band bud explant top Cuttage is held to inducing culture, is cultivated under conditions of being 25 DEG C in temperature, light is carried out to explant daily during culture According to processing；Wherein, the processing method of explant progress photo-irradiation treatment is：Circulation light photograph is carried out with white light, blue light, feux rouges, gold-tinted, The order that the circulation light shines is " white light 30min- blue light 20min- feux rouges 30min- gold-tinted 30min- white light 30min " illumination is total Shi Changwei 7h, the intensity of illumination of the white light is 350Lx, and the intensity of illumination of blue light is 450Lx, and the intensity of illumination of feux rouges is 550Lx, the intensity of illumination of gold-tinted is 350Lx.

(5) squamous subculture：Grown to when the explant of step (4) when height is 6cm and obtain seedling, seedling is gone into subculture training Support in base, being 25 DEG C in temperature is cultivated, and photo-irradiation treatment, photo-irradiation treatment time are carried out to seedling with incandescent lamp daily during supporting For daily 5h, intensity of illumination 800Lx；

(6) culture of rootage：When the seedling of step (5) grows the root system of 2.5cm length, seedling is gently extracted into squamous subculture Base, and the subculture medium residuals for being attached to root are washed with clear water, then seedling is inserted in root media Row culture, and move into culturing rack and cultivated, daytime, cultivation temperature was 28 DEG C, and night cultivation temperature is 17 DEG C and using incandescent Lamp is irradiated to seedling in culturing rack, a length of daily 10h, intensity of illumination 500Lx during irradiation；

(7) hardening：Step (6) is highly put into greenhouse for 11cm seedling and carries out hardening, hardening temperature is 25 DEG C, light According to for solar radiation, relative humidity 15%；

(2) plant：

After hardening 55d, selection cane is sturdy, leaf color is dark green healthy seedling on decomposed plantation plot according to 25cm × 25cm seeding row spacing is planted, and it is 15% that planting process, which irregularly sprays expelling parasite water to keep the relative humidity in plantation plot, directly Harvested to Momordica grosvenori.Wherein, the decomposed method for planting plot is：By fresh white flower pea, fresh alfalfa, fresh butch clover, Rice straw, oil tea slag and fresh Chinese milk vetch are 2 according to mass ratio:2:3:3:4:2 are mixed, then according to 120mg/g addition Measure and the mixed of the cellulase that enzyme activity unit is 1000U/g and the pectase that enzyme activity unit is 900U/g is sprayed into mixture Liquid is closed, the mass ratio of the cellulase and pectase is：1:1.5；Enzymolysis mixture is uniformly shed in breeding after enzymolysis 50h Plant in plot, and cover the soil that last layer thickness is 2.5cm and carry out decomposed, decomposed total duration be 42d, and decomposed temperature is 23 ℃。

Each thimerosal of the present embodiment, culture medium prescription are as follows：

Thimerosal I：

After 12g barbaloin, 3g sodium chloride, 15g Radix et Caulis Opuntiae Dillenii extract and 12g Lotus Leafextract mixing, then Add percentage by volume and be 75% ethanol solution, and solution is settled to 1000mL and is made.

The extracting method of Radix et Caulis Opuntiae Dillenii extract and Lotus Leafextract and embodiment 1 are completely the same in the present embodiment thimerosal I.

Thimerosal II：

By 22g shrubby sophora extract, 4g sodium hydroxide, 4g sodium acid carbonate, 22g triterpene glucoside and 22g poplar After the mixing of willow bark extract, add percentage by volume and be 75% ethanol solution, and solution is settled to 1000mL and is made.

The extracting method of shrubby sophora extract and willow bark extract and embodiment 1 complete one in the present embodiment thimerosal II Cause.

Initial medium：

It is as follows for the MS culture mediums of improvement, formula：

The soybean extraction of 1.5mg/L 6-benzyladenine, 4mg/L aloe polysaccharide, 3mg/L chitosan, 4mg/L Thing, 5g/L agar, the ethanol that 30mL percentage by volumes are 75%, remaining part is MS culture mediums, pH 5.5.

The extracting method of extract of soybean and embodiment 1 are completely the same in the present embodiment initial medium.

Inducing culture：

It is as follows for the MS culture mediums of improvement, formula：

1.5mg/L methyl α-naphthyl acetate, 3mg/L gumbo polysaccharide, 3mg/L cactus polyoses, 4mg/L peanut extract, 6mg/L soybean meal extractive, 5g/L agar, 40mL percentage by volumes are 75% ethanol, and remaining part is MS culture mediums, pH For 5-6.

The extracting method of peanut extract and soybean meal extractive and embodiment 1 complete one in the present embodiment inducing culture Cause.

Subculture medium：

It is as follows for the MS culture mediums of improvement, formula：

The oil tea slag extraction of 1.5mg/L heteroauxin, 3mg/L chitosan, 3mg/L silkworm excrement soaking water, 4mg/L Thing, 4mg/L white mulberry vein-juice, 7mg/L activated carbon, 5g/L agar, 30mL percentage by volumes be 75% ethanol, remaining part For MS culture mediums, pH 5.5.

Silkworm excrement soaking water, oily tealeaf extract, the processing method of white mulberry vein-juice and embodiment 1 in the present embodiment subculture medium It is completely the same.

Root media：

It is as follows for the MS culture mediums of improvement, formula：

1.5mg/L heteroauxin, 1.5mg/L methyl α-naphthyl acetate, 3mg/L chitosan, 3mg/L silkworm excrement soaking water, 3mg/L Extracts from Peanut Hulls, 4mg/L oily tealeaf extract, 5mg/L polygonum flaccidum grass juice factor, 15mg/L activated carbon, 5g/L fine jade Fat, 30mL percentage by volumes are 75% ethanol, and remaining part is MS culture mediums, pH 5.5.

The processing of the present embodiment root media silkworm excrement soaking water, Extracts from Peanut Hulls, oily tealeaf extract, polygonum flaccidum grass juice factor Method and embodiment 1 are completely the same.

Expelling parasite water：

Comprise the following components in parts by weight：12 parts of Radix Sophorae Flavescentis extractive liquid, 7 parts of lucidum extracting liquid, the extraction of 7 parts of eucalyptus Liquid, 12 parts of Radix Astragali extractive solution, the ethanol solution that 40 parts of percentage by volume is 75%, 35 parts of water.

Radix Sophorae Flavescentis extractive liquid, lucidum extracting liquid, eucalyptus extract solution, the extracting method of Radix Astragali extractive solution in the present embodiment expelling parasite water It is completely the same with embodiment 1.

Control group 1：

The other parameters of this control group, method and embodiment 1 are completely the same, but with the mercury that percentage by volume is 0.1% Solution substitutes disinfectant I and carried out disinfection.

Control group 2：

The other parameters of this control group, method and embodiment 1 are completely the same, but with the mercury that percentage by volume is 0.1% Solution substitutes disinfectant II and carried out disinfection.

Control group 3：

The other parameters of this control group, method and embodiment 1 are completely the same, but shine alternative steps using incandescent light (4) light alternate treatment.

Control group 4：

The other parameters of this control group, method and embodiment 1 are completely the same, but use MS culture mediums to substitute initial incubation Base.

Control group 5：

The other parameters of this control group, method and embodiment 1 are completely the same, but use MS culture mediums to substitute Fiber differentiation Base.

Control group 6：

The other parameters of this control group, method and embodiment 1 are completely the same, but use MS culture mediums to substitute squamous subculture Base.

Control group 7：

The other parameters of this control group, method and embodiment 1 are completely the same, but use MS culture mediums to substitute culture of rootage Base.

Control group 8：

The other parameters of this control group, method and embodiment 1 are completely the same, but use clear water base to substitute expelling parasite water.

Control group 9：

The other parameters of this control group, method and embodiment 1 are completely the same, but plant plot without decomposed pre- place Reason, but directly planted after scarifying.

Control group 10：

The other parameters of this control group, method and embodiment 1 are completely the same, but are located in advance without using any disinfectant Reason, using only 75% ethanol soak, moreover, using MS culture mediums substitute initial medium, inducing culture, subculture medium, Root media, disinfectant is substituted with clear water.

Control group 11：

Branch using Momordica grosvenori is directly carrying out cuttage plantation using plot of the method for embodiment 1 after decomposed.

Testing experiment 1：

Determine embodiment 1-3 and control group 1-2, the explant survival rate of control group 10, Hg contents, plantation in explant body 30d plant percents after to plantation plot, concrete condition are shown in Table 1：

Table 1

Group	Hg contents (%)	Explant survival rate (%)	30d plant percents (%)
				Embodiment 1	——	98.54	96.25
Embodiment 2	——	99.26	96.54
				Embodiment 3	——	97.25	95.26
Control group 1	0.08	96.25	93.25
				Control group 2	0.17	96.15	92.26
Control group 10	——	56.25	46.25

As seen from the above table, due to not measuring Hg content in embodiment 1-3, the Hg contents of control group 2 have also appeared richness Collection, illustrating to carry out disinfection using mercury solution can cause Hg to remain, and embodiment 1-3 explant survival rate, 30d plant percents Apparently higher than control group 10, slightly above control group 1-2, conventional mercury can be substituted by illustrating the disinfectant I and disinfectant II of the application Solution, there is good disinfective action to explant.

Testing experiment 2：

Testing example 1-3 and control group 3-11 explant survival rate, 5cm take root shoot survival percent, plantation arrive planting site 30d Survival rate of nursery stock behind 20d plant percents, plantation to plantation plot behind 10d plant percents, plantation to plantation plot after block Rate, specifically it is shown in Table 2：

Table 2

As seen from the above table, embodiment 1-3 and control group 3-9 explant survival rate is more or less the same, hence it is evident that higher than control group 10, illustrate that the disinfectant I of the application, disinfectant II can improve the survival rate of explant；Embodiment 1-3 5cm rooted seedlings survive Rate is more or less the same with control group 10, hence it is evident that higher than control group 3-9, illustrates the initial medium, inducing culture, subculture of the application Culture medium, root media are taken root shoot survival percent with the use of can improve 5cm；Embodiment 1-3 plantation to plantation plot after 20d 30d plant percents are above control group 3-9 behind plant percent, plantation to plantation plot, illustrate using the initial of the application Culture medium, inducing culture, subculture medium, root media are used cooperatively the survival rate that can improve nursery stock 10d, 20d, 30d, Control group 3-9 10d, 20d, 30d survival rate apparently higher than 10 kinds of control group plant plot it is decomposed after, Survival rate of nursery stock can be significantly improved Rate；Embodiment 1-3 10d, 20d, 30d survival rate is higher than control group 11, illustrates to be planted using the tissue-cultured seedling of the application The survival rate of Momordica grosvenori seedling can be improved.

In summary, nursery is carried out to Momordica grosvenori using the method for the present invention, can effectively improves Luohanguo With Plantlets of Tissue Culture Survival rate, and can improves the survival rate of its seedling.

Embodiment described above only expresses the several embodiments of the present invention, and its description is more specific and detailed, but simultaneously Therefore limitation of the scope of the invention can not be interpreted as.It should be pointed out that for the person of ordinary skill of the art, Without departing from the inventive concept of the premise, various modifications and improvements can be made, these belong to the protection model of the present invention Enclose.Therefore, protection scope of the present invention should be determined by the appended claims.

Claims (10)

1. a kind of Momordica grosvenori implantation methods, it is characterised in that methods described includes nursery and plantation step：

(1) nursery：

(1) explant is obtained：Choose without disease pest harm, the Momordica grosvenori spray clip explant buddingged, before explant clip first to Spray spray disinfectant I, and scissors mouth is carried out disinfection with the ethanol solution that percentage by volume is 75%-85%；Spray is gone Except stem section to be cut into the segment for being 2cm-4cm with axillary bud, length with scissors after blade, and segment is put into temperature as -2 DEG C~0 DEG C frozen water in refrigerated；

(2) sterilization of explant：It is 75% that step (1) is refrigerated to the explant after 20min-40min and is put into percentage by volume Soaked in ethanol solution, be put into after draining in thimerosal II and soak 40min-60min, complete disinfecting process；

(3) it is inoculated with：The explant oblique cutting that step (2) disinfects is entered on initial medium, axillary bud upward, is then put culture medium Enter in darkroom and cultivated under conditions of temperature is 24 DEG C -26 DEG C；

(4) Fiber differentiation：After the explant of step (3) grows sterile bud, clip length is 3cm-5cm band bud explant top Cuttage is held to inducing culture, is cultivated under conditions of being 24 DEG C -26 DEG C in temperature, entered daily to explant during culture Row photo-irradiation treatment；

(5) squamous subculture：Grown to when the explant of step (4) when height is 5cm-7cm and obtain seedling, seedling is gone into subculture training Support in base, being 24 DEG C -26 DEG C in temperature is cultivated, and photo-irradiation treatment is carried out to seedling with incandescent lamp daily during supporting, at illumination The reason time is daily 4h-6h, intensity of illumination 700Lx-900Lx；

(6) culture of rootage：When the seedling of step (5) grows the root system of 2cm-3cm length, seedling is gently extracted into squamous subculture Base, and the subculture medium residuals for being attached to root are washed with clear water, then seedling is inserted in root media Row culture, and move into culturing rack and cultivated, daytime, cultivation temperature was 22 DEG C -30 DEG C, and night cultivation temperature is 15 DEG C -20 DEG C And seedling in culturing rack is irradiated using incandescent lamp, a length of daily 8h-12h, intensity of illumination 400Lx- during irradiation 600Lx；

(7) hardening：Step (6) is highly put into greenhouse for 10cm-13cm seedling and carries out hardening, hardening temperature is 22 DEG C- 30 DEG C, illumination is solar radiation, relative humidity 10%-20%；

(2) plant：After hardening 50d-60d, the selection healthy seedling that cane is sturdy, leaf color is dark green is pressed on decomposed plantation plot Planted according to 20cm-30cm × 20cm-30cm seeding row spacing, planting process irregularly sprays expelling parasite water and keeps plantation plot Relative humidity is 10%-20%, until Momordica grosvenori harvests.

A kind of 2. Momordica grosvenori implantation methods according to claim 1, it is characterised in that step (1) the thimerosal I：By The Lotus Leafextract mixing of 10g-15g barbaloin, 2g-5g sodium chloride, 10g-20g Radix et Caulis Opuntiae Dillenii extract and 8g-15g Afterwards, add percentage by volume and be 75% ethanol solution, and solution is settled to 1000mL and is made.

A kind of 3. Momordica grosvenori implantation methods according to claim 1, it is characterised in that step (2) the thimerosal II by 20g-25g shrubby sophora extract, 2g-5g sodium hydroxide, 2g-5g sodium acid carbonate, 20g-25g triterpene glucoside and 20g- After 25g willow bark extract mixing, add percentage by volume and be 75% ethanol solution, and solution is settled to 1000mL It is made.

4. a kind of Momordica grosvenori implantation methods according to claim 1, it is characterised in that step (3) initial medium is The MS culture mediums of improvement：1mg/L-2mg/L 6-benzyladenine, 3mg/L-5mg/L aloe polysaccharide, 2mg/L-4mg/L Chitosan, 3mg/L-6mg/L extract of soybean, 4g/L-6g/L agar, the second that 20mL-40mL percentage by volumes are 75% Alcohol, remaining part are MS culture mediums, pH 5-6.

5. a kind of Momordica grosvenori implantation methods according to claim 1, it is characterised in that step (4) inducing culture is The MS culture mediums of improvement：1mg/L-2mg/L methyl α-naphthyl acetate, 2mg/L-4mg/L gumbo polysaccharide, 2mg/L-4mg/L cactus Polysaccharide, 3mg/L-6mg/L peanut extract, 5mg/L-8mg/L soybean meal extractive, 4g/L-6g/L agar, 30mL- 50mL percentage by volumes are 75% ethanol, and remaining part is MS culture mediums, pH 5-6.

6. a kind of Momordica grosvenori implantation methods according to claim 1, it is characterised in that step (4) explant carries out light Processing method according to processing is：Circulation light photograph is carried out with white light, blue light, feux rouges, gold-tinted, the order that the circulation light shines is " white Light 30min- blue light 20min- feux rouges 30min- gold-tinted 30min- white light 30min " illumination total durations are 6h-9h, the white light Intensity of illumination is 300Lx-400Lx, and the intensity of illumination of blue light is 400Lx-500Lx, and the intensity of illumination of feux rouges is 500Lx- 600Lx, the intensity of illumination of gold-tinted is 300Lx-400Lx.

7. a kind of Momordica grosvenori implantation methods according to claim 1, it is characterised in that step (5) subculture medium is The MS culture mediums of improvement：The silkworm excrement leaching of 1mg/L-2mg/L heteroauxin, 2mg/L-4mg/L chitosan, 2mg/L-4mg/L Soaked, 3mg/L-6mg/L oily tealeaf extract, 3mg/L-6mg/L white mulberry vein-juice, 5mg/L-8mg/L activated carbon, 4g/L- 6g/L agar, 20mL-40mL percentage by volumes are 75% ethanol, and remaining part is MS culture mediums, pH 5-6.

8. a kind of Momordica grosvenori implantation methods according to claim 1, it is characterised in that step (6) root media is The MS culture mediums of improvement：1mg/L-2mg/L heteroauxin, 1mg/L-2mg/L methyl α-naphthyl acetate, 2mg/L-4mg/L chitosan, 2mg/L-4mg/L silkworm excrement soaking water, 2mg/L-4mg/L Extracts from Peanut Hulls, 3mg/L-6mg/L oily tealeaf extract, 3mg/L-6mg/L polygonum flaccidum grass juice factor, 10mg/L-20mg/L activated carbon, 4g/L-6g/L agar, 20mL-40mL volume basis Number is 75% ethanol, and remaining part is MS culture mediums, pH 5-6.

A kind of 9. Momordica grosvenori implantation methods according to claim 1, it is characterised in that the decomposed method in the plantation plot For：According to mass ratio it is 1- by fresh white flower pea, fresh alfalfa, fresh butch clover, rice straw, oil tea slag and fresh Chinese milk vetch 3:1-3:2-4:2-4:3-5:1-3 is mixed, and enzyme is then sprayed into mixture according to 100mg/g-150mg/g addition The mixing for the pectase that the cellulase and enzyme activity unit that unit of activity is 900U/g-1200U/g are 800U/g-1000U/g Liquid, the mass ratio of the cellulase and pectase are：1:1-2；Enzymolysis mixture is uniformly shed after enzymolysis 48h-52h and educated Plant in plot, and cover the soil that last layer thickness is 2cm-3cm and carry out decomposed, decomposed total duration is 40d-45d, decomposed temperature Spend for 22 DEG C -27 DEG C.

10. a kind of Momordica grosvenori implantation methods according to claim 1, it is characterised in that the expelling parasite water is by following parts by weight Composition composition：10 parts -15 parts of Radix Sophorae Flavescentis extractive liquid, 5 parts -10 parts of lucidum extracting liquid, 5 parts -10 parts of eucalyptus extract solution, 7 The Radix Astragali extractive solution of -19 parts of part, the ethanol solution that 35 parts -45 parts of percentage by volume is 75%, 30 parts -40 parts of water.