CN113599998A - Preparation method of wet garbage compost biological deodorant - Google Patents
Preparation method of wet garbage compost biological deodorant Download PDFInfo
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- 239000002781 deodorant agent Substances 0.000 title claims abstract description 31
- 239000002361 compost Substances 0.000 title claims abstract description 24
- 238000002360 preparation method Methods 0.000 title claims abstract description 17
- 238000000855 fermentation Methods 0.000 claims abstract description 72
- 230000004151 fermentation Effects 0.000 claims abstract description 72
- 230000000813 microbial effect Effects 0.000 claims abstract description 11
- 239000002245 particle Substances 0.000 claims abstract description 8
- 239000000945 filler Substances 0.000 claims abstract description 7
- 238000002156 mixing Methods 0.000 claims abstract description 7
- 238000004806 packaging method and process Methods 0.000 claims abstract description 4
- 238000011084 recovery Methods 0.000 claims abstract description 4
- 239000007788 liquid Substances 0.000 claims description 37
- 239000000243 solution Substances 0.000 claims description 19
- 241000894006 Bacteria Species 0.000 claims description 17
- 239000002504 physiological saline solution Substances 0.000 claims description 15
- 239000007789 gas Substances 0.000 claims description 14
- 238000000034 method Methods 0.000 claims description 14
- WQZGKKKJIJFFOK-GASJEMHNSA-N Glucose Natural products OC[C@H]1OC(O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-GASJEMHNSA-N 0.000 claims description 10
- 239000001888 Peptone Substances 0.000 claims description 10
- 108010080698 Peptones Proteins 0.000 claims description 10
- 235000015278 beef Nutrition 0.000 claims description 10
- 238000001035 drying Methods 0.000 claims description 10
- 239000008103 glucose Substances 0.000 claims description 10
- 235000019319 peptone Nutrition 0.000 claims description 10
- 239000000843 powder Substances 0.000 claims description 10
- LSDPWZHWYPCBBB-UHFFFAOYSA-N Methanethiol Chemical compound SC LSDPWZHWYPCBBB-UHFFFAOYSA-N 0.000 claims description 8
- 235000013619 trace mineral Nutrition 0.000 claims description 8
- 239000011573 trace mineral Substances 0.000 claims description 8
- 239000002699 waste material Substances 0.000 claims description 6
- 229920001817 Agar Polymers 0.000 claims description 5
- 235000007340 Hordeum vulgare Nutrition 0.000 claims description 5
- VMHLLURERBWHNL-UHFFFAOYSA-M Sodium acetate Chemical compound [Na+].CC([O-])=O VMHLLURERBWHNL-UHFFFAOYSA-M 0.000 claims description 5
- 235000019764 Soybean Meal Nutrition 0.000 claims description 5
- 241000202221 Weissella Species 0.000 claims description 5
- 239000008272 agar Substances 0.000 claims description 5
- 229910052564 epsomite Inorganic materials 0.000 claims description 5
- 229910000357 manganese(II) sulfate Inorganic materials 0.000 claims description 5
- SQQMAOCOWKFBNP-UHFFFAOYSA-L manganese(II) sulfate Chemical compound [Mn+2].[O-]S([O-])(=O)=O SQQMAOCOWKFBNP-UHFFFAOYSA-L 0.000 claims description 5
- 239000000203 mixture Substances 0.000 claims description 5
- 235000010482 polyoxyethylene sorbitan monooleate Nutrition 0.000 claims description 5
- 229920000053 polysorbate 80 Polymers 0.000 claims description 5
- 239000011734 sodium Substances 0.000 claims description 5
- 235000017281 sodium acetate Nutrition 0.000 claims description 5
- 239000001632 sodium acetate Substances 0.000 claims description 5
- 239000004455 soybean meal Substances 0.000 claims description 5
- 239000010902 straw Substances 0.000 claims description 5
- YWYZEGXAUVWDED-UHFFFAOYSA-N triammonium citrate Chemical compound [NH4+].[NH4+].[NH4+].[O-]C(=O)CC(O)(CC([O-])=O)C([O-])=O YWYZEGXAUVWDED-UHFFFAOYSA-N 0.000 claims description 5
- QGZKDVFQNNGYKY-UHFFFAOYSA-N Ammonia Chemical compound N QGZKDVFQNNGYKY-UHFFFAOYSA-N 0.000 claims description 4
- RWSOTUBLDIXVET-UHFFFAOYSA-N Dihydrogen sulfide Chemical compound S RWSOTUBLDIXVET-UHFFFAOYSA-N 0.000 claims description 4
- 241000233866 Fungi Species 0.000 claims description 4
- 229910000037 hydrogen sulfide Inorganic materials 0.000 claims description 4
- 241000193830 Bacillus <bacterium> Species 0.000 claims description 3
- 241001536371 Bacillus oleronius Species 0.000 claims description 3
- 244000063299 Bacillus subtilis Species 0.000 claims description 3
- 235000014469 Bacillus subtilis Nutrition 0.000 claims description 3
- ZGTMUACCHSMWAC-UHFFFAOYSA-L EDTA disodium salt (anhydrous) Chemical compound [Na+].[Na+].OC(=O)CN(CC([O-])=O)CCN(CC(O)=O)CC([O-])=O ZGTMUACCHSMWAC-UHFFFAOYSA-L 0.000 claims description 3
- 241000588694 Erwinia amylovora Species 0.000 claims description 3
- 241000192130 Leuconostoc mesenteroides Species 0.000 claims description 3
- 229910021380 Manganese Chloride Inorganic materials 0.000 claims description 3
- GLFNIEUTAYBVOC-UHFFFAOYSA-L Manganese chloride Chemical compound Cl[Mn]Cl GLFNIEUTAYBVOC-UHFFFAOYSA-L 0.000 claims description 3
- NINIDFKCEFEMDL-UHFFFAOYSA-N Sulfur Chemical compound [S] NINIDFKCEFEMDL-UHFFFAOYSA-N 0.000 claims description 3
- 239000012153 distilled water Substances 0.000 claims description 3
- 238000009472 formulation Methods 0.000 claims description 3
- 239000011565 manganese chloride Substances 0.000 claims description 3
- 229910052603 melanterite Inorganic materials 0.000 claims description 3
- 229910052717 sulfur Inorganic materials 0.000 claims description 3
- 239000011593 sulfur Substances 0.000 claims description 3
- 229910000368 zinc sulfate Inorganic materials 0.000 claims description 3
- NWONKYPBYAMBJT-UHFFFAOYSA-L zinc sulfate Chemical compound [Zn+2].[O-]S([O-])(=O)=O NWONKYPBYAMBJT-UHFFFAOYSA-L 0.000 claims description 3
- 239000011686 zinc sulphate Substances 0.000 claims description 3
- 235000009529 zinc sulphate Nutrition 0.000 claims description 3
- 229910000396 dipotassium phosphate Inorganic materials 0.000 claims description 2
- 238000011534 incubation Methods 0.000 claims description 2
- 235000010633 broth Nutrition 0.000 claims 9
- 240000005979 Hordeum vulgare Species 0.000 claims 2
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims 1
- 235000019645 odor Nutrition 0.000 abstract description 6
- 230000035899 viability Effects 0.000 abstract description 3
- 241000209219 Hordeum Species 0.000 description 3
- OKKJLVBELUTLKV-UHFFFAOYSA-N Methanol Chemical compound OC OKKJLVBELUTLKV-UHFFFAOYSA-N 0.000 description 3
- 238000012986 modification Methods 0.000 description 2
- 230000004048 modification Effects 0.000 description 2
- 238000010276 construction Methods 0.000 description 1
- 238000012258 culturing Methods 0.000 description 1
- 238000004332 deodorization Methods 0.000 description 1
- 230000000694 effects Effects 0.000 description 1
- 238000005516 engineering process Methods 0.000 description 1
- 230000007613 environmental effect Effects 0.000 description 1
- 239000001963 growth medium Substances 0.000 description 1
- 244000005700 microbiome Species 0.000 description 1
- 238000012216 screening Methods 0.000 description 1
- 239000000126 substance Substances 0.000 description 1
- 238000006467 substitution reaction Methods 0.000 description 1
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01D—SEPARATION
- B01D53/00—Separation of gases or vapours; Recovering vapours of volatile solvents from gases; Chemical or biological purification of waste gases, e.g. engine exhaust gases, smoke, fumes, flue gases, aerosols
- B01D53/34—Chemical or biological purification of waste gases
- B01D53/74—General processes for purification of waste gases; Apparatus or devices specially adapted therefor
- B01D53/84—Biological processes
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/20—Bacteria; Culture media therefor
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A50/00—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE in human health protection, e.g. against extreme weather
- Y02A50/20—Air quality improvement or preservation, e.g. vehicle emission control or emission reduction by using catalytic converters
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Abstract
The invention provides a preparation method of a wet garbage compost biological deodorant, belonging to the technical field of deodorant preparation; the invention comprises the following steps: firstly, preparing microbial fermentation liquor; secondly, inoculating corresponding strains, and performing microbial fermentation to realize strain recovery; step three, uniformly mixing the fermentation products; fourthly, introducing pure malodorous gas; step five, program drying; and sixthly, packaging and storing the dried particles, and putting the particles into a special biological filler. The biological filler prepared from the deodorant disclosed by the invention is convenient to use and strong in microbial viability; and the dried odor can be transported for use, has strong pertinence, and has the characteristic of high removal efficiency on various odors.
Description
Technical Field
The invention relates to an environment-friendly technology, in particular to a preparation method of a wet garbage compost biological deodorant, belonging to the technical field of gas treatment.
Background
The biological trickling filter is widely applied to chemical industry and environmental protection industry. In the prior art, the research on malodorous gas generated in the wet garbage treatment process is limited; especially the targeted biological strain cultivation is carried out according to the component characteristics of the wet garbage and the generated malodorous gas in China.
The malodorous gas generated in the wet garbage treatment process, such as methyl mercaptan, hydrogen sulfide, ammonia gas, methanol and the like, has various varieties and complex components and is difficult to remove.
In the prior art, the preparation method of the biological deodorant comprises the following steps: (1) recovering the strain; (2) preparing seed liquid; (3) preparing solution A and solution B; (4) and mixing the solution A and the solution B according to a specific volume ratio to obtain the biological deodorant.
However, the deodorant in the prior art has the following problems: 1. the culture medium has poor specificity, cannot be applied to all gases, has an unclear application range and has poor suitability for the cultured microorganisms.
And the strains are few in variety, not abundant, inconvenient to store and inconvenient to use.
Disclosure of Invention
The invention provides a novel preparation method of a wet garbage compost biological deodorant, which is used for solving the technical problems of poor biological deodorization and strain use effects in the prior art by setting and culturing different strains for mixing, then screening the strains by environment and then drying the strains.
The preparation method of the wet garbage compost biological deodorant provided by the embodiment of the invention comprises the following steps:
firstly, preparing microbial fermentation liquor;
secondly, inoculating corresponding strains, and performing microbial fermentation to realize strain recovery;
step three, uniformly mixing the fermentation products;
fourthly, introducing pure malodorous gas;
step five, program drying;
and sixthly, packaging and storing the dried particles, and putting the particles into a special biological filler.
The preparation method of the wet garbage compost biological deodorant comprises the following steps of preparing three kinds of fermentation liquor, namely a first fermentation liquor, a second fermentation liquor and a third fermentation liquor in the first step;
the first fermentation liquid is prepared by adding 18-22g of glucose, 16-17g of peptone, 0.5g of beef extract, 18-22g of agar, 1g of straw powder, 3g of highland barley powder and 5g of soybean meal into 1L of physiological saline;
the second fermentation liquid is 1L physiological saline added with 8-12g beef extract, 8-12g peptone, 20g glucose, 2g K2HPO40.5g of MgSO4·7H2O, 0.25g of MnSO4·H2O, 1g tween-80, 1g sodium acetate and adjusted to pH 6.5 using ammonium citrate;
adding 8-12g of Na into 1L of physiological saline as third fermentation liquid2S2O3·5H2O，4gKH2PO44g of K2HPO4，0.5gMgSO4·7H2O，0.4gNH4Cl, and 10mL of a trace element solution;
wherein the formulation of the trace element solution is that 50g of EDTA disodium salt and 22g of ZnSO4 & 7H are added into 1L of distilled water2O, 5g of CaC125.06g of MnCl2·4H2O and 4g of FeSO4·7H2O。
In the second step, the spore bacteria are revived and then added into the first fermentation liquid, the filamentous bacteria are added into the second fermentation liquid, the Weissella bacteria are added into the third fermentation liquid, and the incubator temperature of the three fermentation liquids is kept at 22-28 ℃ for 3 days;
the spore bacteria comprise: one or more of bacillus subtilis, bacillus solitarius, leuconostoc mesenteroides, bacillus amylovorus and bacillus Oleronius;
the filamentous fungi include: one or more of sulfur-producing bacteria, 021 type bacteria and coccobacillus.
The preparation method of the wet garbage compost biological deodorant comprises the step three, wherein the first fermentation liquor, the second fermentation liquor and the third fermentation liquor which are fermentation products are uniformly mixed according to the proportion of 2:2: 1.
The preparation method of the wet garbage compost biological deodorant comprises the step four, wherein in the step four, the uniformly mixed liquid obtained in the step three is placed into a sterile incubator, 100ppm of ammonia gas, 5ppm of hydrogen sulfide and 5ppm of methyl mercaptan are introduced, and the culture is carried out for 1 day.
The preparation method of the wet garbage compost biological deodorant comprises the step four, the liquid evenly mixed in the step three is placed into a sterile incubator, malodorous gas to be treated is introduced, the incubation time is 24-72 hours, and the malodorous gas to be treated is 90-150 ppm.
The preparation method of the wet garbage compost biological deodorant comprises the steps of adding 20g of glucose, 15g of peptone, 0.5g of beef extract, 20g of agar, 1g of straw powder, 3g of highland barley powder and 5g of soybean meal into 1L of physiological saline to prepare a first fermentation solution;
the second fermentation liquid is 1L of physiological saline, and 10g of beef extract, 10g of peptone, 20g of glucose and 2g of K are added2HPO40.5g of MgSO4·7H2O, 0.25g of MnSO4·H2O, 1g tween-80, 1g sodium acetate and adjusted to pH 6.5 using ammonium citrate;
the third fermentation liquid is 1L physiological saline, and 10g Na is added2S2O3·5H2O，4gKH2PO44g of K2HPO4，0.5gMgSO4·7H2O，0.4gNH4Cl, and 10mL of a trace element solution.
In the second step, the spore bacteria are revived and then added into the first fermentation liquid, the filamentous bacteria are added into the second fermentation liquid, the Weissella bacteria are added into the third fermentation liquid, and the incubator temperature of the three fermentation liquids is kept at 25 ℃ for 3 days.
The preparation method of the wet garbage compost biological deodorant is characterized in that the programmed drying is drying by a dryer, the temperature is kept at 65-80 ℃, and the drying time is 0.5-2.5 hours.
The biological filler prepared from the deodorant disclosed by the invention is convenient to use and strong in microbial viability; and the dried odor can be transported for use, has strong pertinence, and has the characteristic of high removal efficiency on various odors.
Drawings
FIG. 1 is a flow chart of a method for preparing a wet waste compost biological deodorant according to an embodiment of the present invention.
Detailed Description
Fig. 1 shows a flow chart of a method for preparing a wet waste compost biological deodorant according to an embodiment of the invention.
The preparation method of the wet garbage compost biological deodorant provided by the embodiment of the invention comprises the following steps:
firstly, preparing microbial fermentation liquor;
specifically, three kinds of fermentation liquor are prepared, namely a first fermentation liquor, a second fermentation liquor and a third fermentation liquor;
the first fermentation liquid is prepared by adding 20g of glucose, 15g of peptone, 0.5g of beef extract, 20g of agar, 1g of straw powder, 3g of highland barley powder and 5g of soybean meal into 1L of physiological saline;
the second fermentation liquid is 1L of physiological saline, and 10g of beef extract, 10g of peptone, 20g of glucose and 2g of K are added2HPO40.5g of MgSO4·7H2O, 0.25g of MnSO4·H2O, 1g tween-80, 1g sodium acetate and adjusted to pH 6.5 using ammonium citrate;
the third fermentation liquid is 1L physiological saline, and 10g Na is added2S2O3·5H2O，4gKH2PO44g of K2HPO4，0.5gMgSO4·7H2O，0.4gNH4Cl, and 10mL of a trace element solution;
wherein the formulation of the trace element solution is that 50g of EDTA disodium salt and 22g of ZnSO4 & 7H are added into 1L of distilled water2O, 5g of CaC125.06g of MnCl2·4H2O and 4g of FeSO4·7H2O。
Secondly, inoculating corresponding strains, and performing microbial fermentation to realize strain recovery; specifically, the spore bacteria are revived and then added into the first fermentation liquid, the filamentous bacteria are added into the second fermentation liquid, the Weissella bacteria are added into the third fermentation liquid, and the temperature of an incubator for the three fermentation liquids is kept at 25 ℃ for 3 days;
the spore bacteria comprise: one or more of bacillus subtilis, bacillus solitarius, leuconostoc mesenteroides, bacillus amylovorus and bacillus Oleronius;
the filamentous fungi include: one or more of sulfur-producing bacteria, 021 type bacteria and coccobacillus.
Step three, uniformly mixing the fermentation products; and uniformly mixing the first fermentation liquid, the second fermentation liquid and the third fermentation liquid of the fermentation product according to the ratio of 2:2: 1.
Fourthly, introducing pure malodorous gas; putting the uniformly mixed liquid in the third step into a sterile incubator, and introducing malodorous gas to be treated for 24-72 hours, wherein the malodorous gas to be treated is 90-150 ppm;
in general, it is preferable to introduce 100ppm of ammonia gas, 5ppm of hydrogen sulfide, and 5ppm of methyl mercaptan depending on the composition of wet garbage, and to culture them for 1 day.
Step five, program drying; the procedure drying is drying by a dryer, the temperature is kept at 65-80 ℃, and the drying time is 0.5-2.5 hours.
And sixthly, packaging and storing the dried particles, and putting the particles into a special biological filler.
The biological filler prepared from the deodorant disclosed by the invention is convenient to use and strong in microbial viability; and the dried odor can be transported for use, has strong pertinence, and has the characteristic of high removal efficiency on various odors.
The above-mentioned serial numbers of the embodiments of the present invention are merely for description and do not represent the merits of the embodiments. Through the above description of the embodiments, those skilled in the art will clearly understand that the above embodiment method can be implemented by some modifications plus the necessary general technical overlap; of course, the method can also be realized by simplifying some important technical features in the upper level. Based on such understanding, the technical solution of the present invention essentially or contributing to the prior art is: overall function and construction, and to cooperate with the structure described in the various embodiments of the present invention.
Finally, it should be noted that: the above embodiments are only used to illustrate the technical solution of the present invention, and not to limit the same; while the invention has been described in detail and with reference to the foregoing embodiments, it will be understood by those skilled in the art that: the technical solutions described in the foregoing embodiments may still be modified, or some or all of the technical features may be equivalently replaced; and the modifications or the substitutions do not make the essence of the corresponding technical solutions depart from the scope of the technical solutions of the embodiments of the present invention.
Claims (9)
1. A preparation method of a wet garbage compost biological deodorant is characterized by comprising the following steps:
firstly, preparing microbial fermentation liquor;
secondly, inoculating corresponding strains, and performing microbial fermentation to realize strain recovery;
step three, uniformly mixing the fermentation products;
fourthly, introducing malodorous gas to be treated;
step five, program drying;
and sixthly, packaging and storing the dried particles, and putting the particles into a special biological filler.
2. A method for preparing a biological deodorant for wet waste composts as claimed in claim 1, wherein in the first step, three kinds of fermentation liquors are prepared, which are a first fermentation liquor, a second fermentation liquor and a third fermentation liquor, respectively;
the first fermentation liquid is prepared by adding 18-22g of glucose, 16-17g of peptone, 0.5g of beef extract, 18-22g of agar, 1g of straw powder, 3g of highland barley powder and 5g of soybean meal into 1L of physiological saline;
the second fermentation liquid is 1L physiological saline added with 8-12g beef extract, 8-12g peptone, 20g glucose, 2g K2HPO40.5g of MgSO4·7H2O, 0.25g of MnSO4·H2O, 1g tween-80, 1g sodium acetate and adjusted to pH 6.5 using ammonium citrate;
adding 8-12g of Na into 1L of physiological saline as third fermentation liquid2S2O3·5H2O，4gKH2PO44g of K2HPO4，0.5gMgSO4·7H2O，0.4gNH4Cl, and 10mL of a trace element solution;
wherein the formulation of the trace element solution is that 50g of EDTA disodium salt and 22g of ZnSO4 & 7H are added into 1L of distilled water2O, 5g of CaC125.06g of MnCl2·4H2O and 4g of FeSO4·7H2O。
3. A method for preparing a wet garbage compost biological deodorant as claimed in claim 2, wherein in the second step, spore bacteria are revived and added to the first fermentation broth, filamentous bacteria are added to the second fermentation broth, weissella bacteria are added to the third fermentation broth, and the incubator temperature of the three fermentation broths is maintained at 22-28 ℃ for 3 days;
the spore bacteria comprise: one or more of bacillus subtilis, bacillus solitarius, leuconostoc mesenteroides, bacillus amylovorus and bacillus Oleronius;
the filamentous fungi include: one or more of sulfur-producing bacteria, 021 type bacteria and coccobacillus.
4. A method for preparing a wet waste compost biological deodorant as claimed in claim 3, wherein in the third step, the first fermentation liquid, the second fermentation liquid and the third fermentation liquid which are fermentation products are uniformly mixed in a ratio of 2:2: 1.
5. The method for preparing a biological deodorant for wet waste composts as claimed in claim 4, wherein in the fourth step, the mixed liquid obtained in the third step is placed in a sterile incubator, 100ppm of ammonia gas, 5ppm of hydrogen sulfide and 5ppm of methyl mercaptan are introduced, and the mixture is incubated for 1 day.
6. The preparation method of the wet garbage compost biological deodorant as claimed in claim 4, wherein in the fourth step, the liquid mixed in the third step is put into a sterile incubator, malodorous gas to be treated is introduced, the incubation time is 24-72 hours, and the malodorous gas to be treated is 90-150 ppm.
7. The method for preparing a wet garbage compost biological deodorant as claimed in claim 2, wherein the first fermentation solution is prepared by adding 20g of glucose, 15g of peptone, 0.5g of beef extract, 20g of agar, 1g of straw powder, 3g of highland barley powder and 5g of soybean meal into 1L of physiological saline water;
the second fermentation liquid is 1L of physiological saline, and 10g of beef extract, 10g of peptone, 20g of glucose and 2g of K are added2HPO40.5g of MgSO4·7H2O, 0.25g of MnSO4·H2O, 1g tween-80, 1g sodium acetate and adjusted to pH 6.5 using ammonium citrate;
the third fermentation liquid is 1L physiological saline, and 10g Na is added2S2O3·5H2O，4gKH2PO44g of K2HPO4，0.5gMgSO4·7H2O，0.4gNH4Cl, and 10mL of a trace element solution.
8. A method of producing a wet refuse compost biological deodorant as claimed in claim 3, wherein in said second step, spores are revived and added to the first fermentation broth, filamentous fungi are added to the second fermentation broth, weissella is added to the third fermentation broth, and the incubator temperature of the three fermentation broths is maintained at 25 ℃, and the three fermentation broths are cultured for 3 days.
9. A method of preparing a wet waste compost biological deodorant as claimed in claim 1, wherein said programmed drying is dryer drying, the temperature is maintained at 65-80 ℃, and the drying time is 0.5-2.5 hours.
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Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102068711A (en) * | 2010-03-26 | 2011-05-25 | 无锡中佳科技股份有限公司 | Microbial deodorant as well as preparation method and application thereof |
| CN104310571A (en) * | 2014-10-11 | 2015-01-28 | 昆明理工大学 | Method for purifying poisonous and harmful organic pollutants by using electromagnetic collaborative enhanced biological packed tower |
| CN105709593A (en) * | 2016-03-23 | 2016-06-29 | 凯天环保科技股份有限公司 | Compact biological purification module |
| CN109609394A (en) * | 2018-12-26 | 2019-04-12 | 大连雪龙都市农业有限公司 | A kind of deodorization fermentative microorganism microbial inoculum and the preparation method and application thereof |
| CN111686287A (en) * | 2020-06-30 | 2020-09-22 | 北京星皓互动生物科技有限公司 | Preparation method and application of microbial bacteriostatic deodorant |
-
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Patent Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102068711A (en) * | 2010-03-26 | 2011-05-25 | 无锡中佳科技股份有限公司 | Microbial deodorant as well as preparation method and application thereof |
| CN104310571A (en) * | 2014-10-11 | 2015-01-28 | 昆明理工大学 | Method for purifying poisonous and harmful organic pollutants by using electromagnetic collaborative enhanced biological packed tower |
| CN105709593A (en) * | 2016-03-23 | 2016-06-29 | 凯天环保科技股份有限公司 | Compact biological purification module |
| CN109609394A (en) * | 2018-12-26 | 2019-04-12 | 大连雪龙都市农业有限公司 | A kind of deodorization fermentative microorganism microbial inoculum and the preparation method and application thereof |
| CN111686287A (en) * | 2020-06-30 | 2020-09-22 | 北京星皓互动生物科技有限公司 | Preparation method and application of microbial bacteriostatic deodorant |
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