CN105754888A - Bacillus licheniformis and microbial agent and their application in fermentation bed culture - Google Patents
Bacillus licheniformis and microbial agent and their application in fermentation bed culture Download PDFInfo
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Abstract
The invention provides Bacillus licheniformis, collected under CGMCC No.11235.The invention also provides a microbial agent comprising the Bacillus licheniformis described herein as an effective ingredient.The invention also provides the application of the Bacillus licheniformis described herein and the microbial agent described herein in fermentation bed culture.Through the above-mentioned technical scheme, it is possible to more effectively remove stink of livestock and poultry.
Description
Technical field
The present invention relates to agricultural biological technical field, in particular it relates to a kind of Bacillus licheniformis, a kind of microbial bacterial agent and their application in fermentation bed cultivation.
Background technology
In recent years; China's livestock culture industry development is swift and violent; and tend to scale gradually, intensive; but owing to sewage disposal facility and the fecaluria treatment measures of domestic livestock and poultry farm all lag far behind developed country from technology and scale; the air of surrounding area, soil and water body are all caused serious pollution by the waste water discharged from plant, waste gas, rubbish etc., wherein especially notable with the fowl and livestock farm foul gas sense organ to poultry and surrounding population and health effect.Livestock and poultry farm foul gas complicated component, the method for conventional process foul gas is that duty is swept, and ventilates more, rushes with water, and this method wastes substantial amounts of water resource, makes plant's humidity increase simultaneously, is easily caused poultry ill.
Adopting fermentation bed cultivation technology is the new method solving livestock culture fecal pollution.The principle of fermentation bed cultivation technology is to utilize microbial bacterial agent, mix with bedding and padding such as sawdust, straw, rice husks by a certain percentage, microorganism is bred with fowl and animal excrement for nutrition, the Organic substance in fowl and animal excrement is made to be decomposed fully and convert, so that stink disappears, and microbial growth and breeding provide the nutrient substance such as tropina to poultry simultaneously, thus formed one pollution-free, without discharge, without the cultivating system of foul smell.
Current domestic fermentation bed cultivation pattern popularization and application in the breeding production such as pig, but existing fermentation bed strain there is also decomposition, and feces efficiency is low, the problems such as strain working life is short, easy death, decomposition removal effect for ammonia main in plant's foul gas and hydrogen sulfide gas is poor, and deodorizing effect is inconspicuous.
Summary of the invention
In order to overcome the defects such as the decomposition feces efficiency existing for existing fermentation bed strain is low, the invention provides and a kind of decompose the Bacillus licheniformis that feces is in hgher efficiency.
The present inventor has separated a bacillus licheniformis, find that ammonium nitrogen can be converted into nitrite nitrogen thus reducing the generation of ammonia nitrogen by efficiently, also there is the product acid activity of excellence simultaneously, it is possible to maintain the acid condition of strain own growth environment, resulting in the present invention.
The present invention provides a kind of Bacillus licheniformis, and the deposit number of this Bacillus licheniformis (Bacilluslicheniformis) is CGMCCNo.11235.
Present invention also offers a kind of microbial bacterial agent, this microbial bacterial agent contains thalline and culture medium, and described thalline contains the Bacillus licheniformis that deposit number is CGMCCNo.11235.
Present invention also offers Bacillus licheniformis as above and the microbial bacterial agent as above application in fermentation bed cultivation.
By technique scheme, the present invention can maintain the low ph conditions of growth, extends the working life of strain, and the ammonia nitrogen simultaneously degraded in fowl and animal excrement more efficiently, thus alleviating plant's foul smell.
Other features and advantages of the present invention will be described in detail in detailed description of the invention part subsequently.
Biomaterial preservation
The Bacillus licheniformis of the present invention is the pure culture that the present inventor separates from the cattle manure Chicken Manure Compost Product samples that Yanqing County of Beijing gathers, its deposit number is CGMCCNo.11235, preservation date is on August 13rd, 2015, depositary institution is China Committee for Culture Collection of Microorganisms's common micro-organisms center, address is positioned at Yard 1, BeiChen xi Road, Chaoyang District, Beijing City 3 Institute of Microorganism, Academia Sinica, and Classification And Nomenclature is Bacillus licheniformis (Bacilluslicheniformis).
Detailed description of the invention
Hereinafter the specific embodiment of the present invention is described in detail.It should be appreciated that detailed description of the invention described herein is merely to illustrate and explains the present invention, it is not limited to the present invention.
The invention provides a kind of Bacillus licheniformis, the deposit number of this Bacillus licheniformis (Bacilluslicheniformis) is CGMCCNo.11235.
Present invention also offers a kind of microbial bacterial agent, this microbial bacterial agent contains thalline and culture medium, and described thalline contains the Bacillus licheniformis that deposit number is CGMCCNo.11235.
Wherein, the amount of the thalline contained in microbial bacterial agent can in very large range change, for instance in every gram of microbial bacterial agent, and deposit number is the viable count of the Bacillus licheniformis of CGMCCNo.11235 can be 108-1011Cfu, it is preferable that in situation, in every gram of microbial bacterial agent, deposit number is the viable count of the Bacillus licheniformis of CGMCCNo.11235 can be 109-1010cfu。
According to the present invention, the kind of described culture medium can in very large range change, can be the various culture medium that can be used in cultivating Bacillus licheniformis, can be commercially available or record according to " microbiological culture media handbook " (MicrobiologyCultureMediaManual) prepares for example, it is possible to make above-mentioned culture medium for the conventional culture medium such as beef-protein medium, nutrient broth medium, LB culture medium.Such as, culture medium can be beef-protein medium, and it contains the sodium chloride of the Carnis Bovis seu Bubali cream of 1-5g/L, the peptone of 5-20g/L and 3-8g/L.
Wherein, above-mentioned various culture medium can sterilizing methods conventionally carry out sterilizing after standby, for instance 115-125 DEG C and sterilizing 10-30 minute when 1.5-2 normal atmosphere.
Wherein, the preparation method of described microbial bacterial agent may include that to be inoculated in culture medium the bacillus licheniformis that deposit number is CGMCCNo.11235 and cultivates.Under preferable case, making in the every gram of described microbial bacterial agent obtained by cultivating, deposit number is the viable count of the bacillus licheniformis of CGMCCNo.11235 can be 107-1011Cfu, more preferably 109-1010cfu.In the process cultivated, it is possible to by conventional method, for instance blood cell plate counting method or OD value observational method obtain the concentration of viable bacteria.
Bacterium solution after cultivation can use directly as microbial bacterial agent, it is preferable that in situation, and bacterium solution uses by including the microbial bacterial agent of the dosage form that the step such as aseptic filtration, lyophilization is further processed into more convenient storage.Wherein, the condition of culture of strain has no particular limits, it is possible to for typical conditions in Bacillus licheniformis incubation, for instance adopting shaking table concussion to cultivate, cultivation temperature can be 28-37 DEG C, and incubation time can be 1-3 days.
Present invention also offers Bacillus licheniformis as above and microbial bacterial agent as above is applied to bedding and padding pre fermentation in fermentation bed cultivation.
According to the present invention, the not special requirement of Bacillus licheniformis and microbial bacterial agent application process in fermentation bed cultivation technology, it can be the method that is normally applied of microbial inoculum in fermentation bed cultivation, such as, bedding and padding and microbial inoculum are mixed in proportion, the bedding and padding heap fermentation that will stir after adjusting moisture.
According to the present invention, fermentation bed contains bedding and padding and microbial bacterial agent, and wherein, the weight ratio of bedding and padding and microbial bacterial agent can in very large range change, it is preferable that in situation, the weight of microbial bacterial agent and bedding and padding can ratio for 1:(900-1200).
According to the present invention, bedding and padding requirement not special in the hybrid mode of microbial inoculum, it is possible to bedding and padding being piled up in proportion, then microbial inoculum is sprinkling upon on bedding and padding equably, being sufficiently mixed until mixing completely.Bedding and padding adjust the mode of moisture also without special requirement, for instance, it is possible to add water in bedding and padding with the process of microbial inoculum mixing, be sufficiently mixed uniformly simultaneously.
According to the present invention, the not special requirement of the moisture content of bedding and padding, it is possible to for the moisture content of bedding and padding in normal fermentation bed cultural technique.Under preferable case, the water content of bedding and padding can be 40-50 weight %, and the method for testing of water content bedding and padding within the scope of this can grab bedding and padding for hands can be agglomerating, looses one's grip and namely dissipates, and has the sensation of water on hand but webs are anhydrous oozes out.
According to the present invention, the not special requirement of bedding and padding kind, it is possible to for bedding and padding kind conventional in fermentation bed cultivation technology, it is preferable that in situation, bedding and padding can be one or more in sawdust, rice husk and wheat bran.
Wherein, as a kind of preferred implementation of the present invention, bedding and padding can be the mixture of sawdust, rice husk and wheat bran, and relative to the sawdust of 100 weight portions, the consumption of rice husk can be 60-70 weight portion, and the consumption of wheat bran can be 5-10 weight portion.Microbial bacterial agent described above can first be mixed homogeneously with wheat bran, and the two mixture fully mixes with the mixture of sawdust and rice husk again, and the adjustment bedding and padding water content that simultaneously adds water is to 40-50 weight %.In the preferred embodiment, can allowing on the one hand Bacillus licheniformis fast-growth during the fermentation, the fermenting bed padding obtained on the other hand is used for cultivating poultry and can more effectively remove cultivation stink.
According to the present invention, bedding and padding mix homogeneously and after regulating water content can heap fermentation, wherein, the special requirement of the size of bedding and padding heap, as long as meeting its internal temperature can make microbial bacterial agent normal fermentation, it is preferable that in situation, padding material stack volume can at 10m3Above, high more than 1.5m piled by bedding and padding.In order to increase the heat insulation effect in bedding and padding heap sweat, it is possible to cover straw mattress or woven bag on bedding and padding are piled.
According to the present invention, above-mentioned fermentation bed cultivation can include at least one in fermentation bed to raise pig, fermentation bed poultry, fermentation bed duck culturing and fermentation bed sheep raising.
The present invention is further described below in conjunction with embodiment:
Embodiment 1
The preparation of the present embodiment cultivation and microbial bacterial agent for the Bacillus licheniformis of the present invention is described.
The Bacillus licheniformis (Bacilluslicheniformis) that deposit number is CGMCCNo.11235 is inoculated in LB culture medium (NaCl of tryptone containing 10g/L, the yeast extract of 5g/L and 10g/L), cultivates under vibrating at 30 DEG C and 200 revs/min.The bacterium solution of gained is the microbial bacterial agent of the present embodiment, and in every gram of this microbial bacterial agent, viable count is 109cfu。
Embodiment 2
The preparation of the present embodiment cultivation and microbial bacterial agent for the Bacillus licheniformis of the present invention is described.
The Bacillus licheniformis (Bacilluslicheniformis) that deposit number is CGMCCNo.11235 is inoculated in beef-protein medium (NaCl of peptone containing 20g/L, the Carnis Bovis seu Bubali cream of 5g/L and 5g/L), cultivates under vibrating at 37 DEG C and 180 revs/min and obtain bacterium solution.Namely the bacterium solution of gained obtains the microbial bacterial agent of the present embodiment after aseptic filtration, lyophilization, and in every gram of this microbial bacterial agent, viable count is 1010cfu。
Embodiment 3
The preparation of the present embodiment cultivation and microbial bacterial agent for the Bacillus licheniformis of the present invention is described.
The Bacillus licheniformis (Bacilluslicheniformis) that deposit number is CGMCCNo.11235 is inoculated in nutrient broth medium (NaCl of peptone containing 10g/L, the Carnis Bovis seu Bubali cream powder of 3g/L and 5g/L), cultivates under vibrating at 35 DEG C and 160 revs/min and obtain bacterium solution.Namely the bacterium solution of gained obtains the microbial bacterial agent of the present embodiment after aseptic filtration, lyophilization, and in every gram of this microbial bacterial agent, viable count is 6 × 109cfu。
Comparative example 1
By the article number purchased from ATCC it is14580TMBacillus licheniformis (Bacilluslicheniformis) be inoculated in LB culture medium (NaCl of tryptone containing 10g/L, the yeast extract of 5g/L and 10g/L), cultivate under vibrating at 35 DEG C and 120 revs/min and obtain bacterium solution.Namely the bacterium solution of gained obtains the microbial bacterial agent of the present embodiment after aseptic filtration, lyophilization, and in every gram of this microbial bacterial agent, viable count is 5 × 109cfu。
Comparative example 2
This comparative example is for illustrating microbial bacterial agent different from the present invention and preparation method thereof.(1) 100 weight portions nutrient culture medium (content of peptone be 0.8 weight %, NaCl content be 0.4 weight %, Carnis Bovis seu Bubali cream content be 0.45 weight %, surplus be sterilized water, pH is 7.0) in the bacterial strain (ACCC10146 of Bacillus licheniformis of inoculation 4 weight portions, purchased from Chinese agriculture Culture Collection), 37 DEG C of fermentation culture, it is sampled during the fermentation and is observed by ascites method, until the viable count of Bacillus licheniformis is 0.8 × 1010Individual/gram；(2) at the bacterial strain (ACCC10629 of the bacillus subtilis of nutrient inoculation of medium 2 weight portion of 100 weight portions, purchased from Chinese agriculture Culture Collection), 37 DEG C of fermentation culture, it is sampled during the fermentation and is observed by ascites method, until the viable count of bacillus subtilis is 0.8 × 1010Individual/gram culture medium, wherein, in described bouillon media, the content of peptone be 1 weight %, NaCl content be 0.25 weight %, Carnis Bovis seu Bubali cream content be 0.45 weight %, surplus be sterilized water, pH is 7.0.(3) at the bacterial strain (ACCC10113 of the Bacillus pumilus of nutrient inoculation of medium 3 weight portion of 100 weight portions, purchased from Chinese agriculture Culture Collection), 37 DEG C of fermentation culture, it is sampled during the fermentation and is observed by ascites method, until the viable count of Bacillus pumilus is 0.8 × 1010Individual/gram, wherein, in described bouillon media, the content of peptone be 0.5 weight %, NaCl content be 0.5 weight %, Carnis Bovis seu Bubali cream content be 0.3 weight %, surplus be sterilized water, pH is 7.0.(4) at the bacterial strain (ACCC10229 of the Bacillus coagulans of YPG inoculation of medium 4 weight portion of 100 weight portions, purchased from Chinese agriculture Culture Collection), 37 DEG C of fermentation culture, it is sampled during the fermentation and is observed by ascites method, until the viable count of Bacillus coagulans is 0.8 × 1010Individual/gram, wherein, in described YPG culture medium, the content of peptone be 0.8 weight %, glucose content be 0.3 weight %, yeast extract content be 0.8 weight %, surplus be sterilized water, the pH of this culture medium is 7.2.(5) Wine brewing yeast strain (ACCC20237 of inoculation 3 weight portions in the malt extract medium (Shanghai crystalline substance pure reagent company limited) of 100 weight portions, purchased from Chinese agriculture Culture Collection), 30 DEG C of fermentation culture, it is sampled during the fermentation and is observed by ascites method, until the viable count of saccharomyces cerevisiae is 0.8 × 1010Individual/gram culture medium.(6) bacterial strain (ACCC40126 of the Streptomyces jingyangensis of inoculation of medium 4 weight portion is synthesized at the Gao Shi of 100 weight portions, purchased from Chinese agriculture Culture Collection), 30 DEG C of fermentations, it is sampled during the fermentation and is observed by ascites method, until the viable count of Streptomyces jingyangensis is 0.8 × 1010Individual/gram, wherein, in described Gause I culture medium, with the gross weight of this culture medium for benchmark, the content of soluble starch is 2 weight %, KNO3Content be 0.2 weight %, K2HPO4Content be 0.7 weight %, NaCl content be 0.03 weight %, surplus be sterilized water, the pH of this culture medium is 7.2.(7) Bacillus licheniformis that will obtain in step (1) to (6), bacillus subtilis, Bacillus pumilus, Bacillus coagulans, saccharomyces cerevisiae and Streptomyces jingyangensis proportionally mix, make in the microbial bacterial agent obtained, with total viable count of microbial bacterial agent of obtaining for benchmark, the viable count of Bacillus licheniformis is 15 weight % of total viable count, the viable count of bacillus subtilis is the 15% of total viable count, the viable count of Bacillus pumilus is the 20% of total viable count, the viable count of Bacillus coagulans is the 20% of total viable count, the viable count of saccharomyces cerevisiae is the 15% of total viable count, the viable count of Streptomyces jingyangensis is the 15% of total viable count.
Embodiment 4
The present embodiment is used for illustrating that microbial bacterial agent of the present invention is for the pre-fermented method of bedding and padding in fermentation bed cultivation.The present embodiment uses the microbial bacterial agent identical with embodiment 3.
Take the Masson Pine sawdust of 100 weight portions and the rice husk of 66 weight portions, it is spilled into 20 weight parts waters, mix homogeneously is also piled, after the microbial inoculum mix homogeneously of the wheat bran of 6 weight portions and 0.17 weight portion, uniformly it is sprinkling upon on the sawdust and rice husk piled up, being fully mixed to by all raw materials completely uniformly, adjust moisture to bedding and padding water content is 45% simultaneously, and mixed bedding and padding are by often piling 10m3, high 1.5m piles up, and bedding and padding covers straw mattress heat-preservation fermentation, as bedding and padding temperature > 55 DEG C, can use after spreading out.
Embodiment 5
The present embodiment is used for illustrating that microbial bacterial agent of the present invention is for the pre-fermented method of bedding and padding in fermentation bed cultivation.The present embodiment uses the microbial bacterial agent identical with embodiment 2.
Take the Masson Pine sawdust of 100 weight portions and the rice husk of 60 weight portions, it is spilled into 20 weight parts waters, mix homogeneously is also piled, after the microbial inoculum mix homogeneously of the wheat bran of 10 weight portions and 0.14 weight portion, uniformly it is sprinkling upon on the sawdust and rice husk piled up, being fully mixed to by all raw materials completely uniformly, adjust moisture to bedding and padding water content is 40% simultaneously, and mixed bedding and padding are by often piling 10m3, high 1.5m piles up, and bedding and padding covers straw mattress heat-preservation fermentation, as bedding and padding temperature > 55 DEG C, can use after spreading out.
Embodiment 6
The present embodiment is used for illustrating that microbial bacterial agent of the present invention is for the pre-fermented method of bedding and padding in fermentation bed cultivation.The present embodiment uses the microbial bacterial agent identical with embodiment 1.
Take the Masson Pine sawdust of 100 weight portions and the rice husk of 70 weight portions, it is spilled into 20 weight parts waters, mix homogeneously is also piled, after the microbial inoculum mix homogeneously of 0.21 weight portion, uniformly it is sprinkling upon on the sawdust and rice husk piled up, being fully mixed to by all raw materials completely uniformly, adjust moisture to bedding and padding water content is 50% simultaneously, and mixed bedding and padding are by often piling 10m3, high 1.5m piles up, and bedding and padding covers straw mattress heat-preservation fermentation, as bedding and padding temperature > 55 DEG C, can use after spreading out.
Comparative example 3
Adopt the bedding and padding pre fermentation method in embodiment 4, institute the difference is that, use the microbial bacterial agent identical with comparative example 1.
Comparative example 4
This comparative example, for the pad material fermentation method different from the present invention is described, uses the microbial bacterial agent identical with comparative example 2.
(1) preparing bedding and padding according to following weight ratio: the consumption of rice straw is 20 weight %, the consumption of rice husk is 30 weight %, and the consumption of wood flour is 40 weight %, and the consumption of Testa oryzae is 10 weight %.(2) using the microbial bacterial agent that comparative example 2 prepares that described bedding and padding are inoculated, relative to the base material of 100 weight portions, the inoculum concentration of microbial bacterial agent is 3 weight portions.(3) postvaccinal bedding and padding being fermented, fermentation temperature is 50 DEG C, and fermentation time is 2 days.(4) bedding and padding after fermentation being carried out windrow in pig house, the height of windrow is 55 centimeters, and in windrow, temperature maintains 35-60 DEG C, and the use time of these bedding and padding is 150 days, and the temperature of pig house maintains 15-35 DEG C.
Testing example 1
This testing example measures the ability of the degradation of ammonia nitrogen of the microbial bacterial agent of embodiment 1-3 and comparative example 1-2.
Prepare aseptic beef-protein medium respectively, the conical flask of 250mL loads 100mL culture medium, is separately added into the microbial bacterial agent 0.05g of embodiment 1-3 and comparative example 1, be placed in 37 DEG C and cultivate 24h.
Preparation ammonia oxidation bacteria culture medium ((NH4)2SO42g/L, NaCl0.3g/L, FeSO4·7H2O0.03g/L, MgSO4·7H2O0.03g/L, K2HPO41g/L, NaHCO31.6g/L, agar powder 18g/L, use 1N sodium hydroxide solution to adjust pH to 7.2), every bottle of 100mL, accesses the above-mentioned each bacterium solution of 1mL.After cultivating 4 days, the concentration of ammonia nitrogen in detection culture medium, result is listed in table 1.
Table 1
Data according to table 1 are visible, and the deposit number of the present invention is the Bacillus licheniformis (Bacilluslicheniformis) of CGMCCNo.11235 and microbial inoculum has the ability of prominent degradation of ammonia nitrogen relative to other microorganisms and microbial inoculum.
Testing example 2
This testing example measures the acid producing ability of the microbial bacterial agent of embodiment 1-3 and comparative example 1-2.
Prepare aseptic beef-protein medium respectively, the conical flask of 250mL loads 100mL culture medium, is separately added into the microbial bacterial agent 0.05g of embodiment 1-3 and comparative example 1, be placed in 37 DEG C and cultivate 24h.
Preparation acid-producing bacteria culture medium (glucose 6g/L, yeast extract 1g/L, peptone 1g/L, MgSO40.05g/L, CaCO31g/L, bromocresol purple 0.04g/L), acid-producing bacteria culture medium 8mL is placed in teat glass (18mm*180mm), sterilizing is good standby.Access the above-mentioned each bacterium solution of 1mL, be placed in 37 DEG C, 170r/min shake cultivation.Seeing whether to make bromocresol purple variable color after cultivating 24h, and measure the pH value in each sample tube, result is listed in table 2.
Table 2
| Microbial inoculum | Whether variable color | pH |
| Embodiment 1 | It is | 5.12 |
| Embodiment 2 | It is | 5.01 |
| Embodiment 3 | It is | 4.93 |
| Comparative example 1 | No | 7.09 |
| Comparative example 2 | No | 6.83 |
Data according to table 2 are visible, the deposit number of the present invention is the Bacillus licheniformis (Bacilluslicheniformis) of CGMCCNo.11235 and microbial inoculum has stronger acid producing ability relative to other microorganisms and microbial inoculum, it is possible to maintain the sour environment needed for growth of microorganism.
Testing example 3
This testing example measures the performance of fermentation bed in embodiment 4-6 and comparative example 3-4 bedding and padding pre fermentation method.The test pH value of bedding and padding, temperature and total number of bacteria, result is listed in table 3 respectively, table 4 and in table 5.The method of testing of pH value is: take the air-dry sample being equivalent to the fresh sample of 2g, adds 50mL distilled water, at shaking table vibration mixing 20min, stands 2h, measures the pH value of supernatant；The assay method of temperature is in 1 meter of ground of stockpile distance eminence, inserts thermometer from ramped surfaces to stockpile central vertical, is that 30cm, 60cm and 90cm place long handle metallic thermometer measures temperature in the degree of depth, averages；The assay method of total number of bacteria is dilution spread flat band method.
Table 3
Data according to table 3 are visible, and the microbial inoculum of the present invention can the pH value of dash adjustment fermentation bedding and padding so that it is maintaining the condition of phase centering, this is conducive to the flourish of probiotic bacteria, is conducive to the rapid-digestion of feces of livestock and poultry when using in poultry house.
Table 4
Data according to table 4 are it can be seen that the microbial inoculum of the present invention adds in fermenting bed padding, and fermentation calefaction speed is fast, ferments and can enter the megathermal period on the 3rd day, and this illustrates that this bacteria fermentation speed is fast, and efficiency is high.Can be seen that from the Data Comparison of embodiment 4-5 Yu embodiment 6, it is preferred that fermenting bed padding be sawdust, rice husk and wheat bran be used in conjunction with preparation fermentation bed when, the fermenting speed of microbial inoculum is faster.
Table 5
Data according to table 5 are visible, compared with comparative example 3-4, in the microbial bacterial agent that embodiment 4-6 uses, cultivable bacteria sum is higher, and the predominant number of the Bacillus licheniformis that deposit number is CGMCCNo.11235 (Bacilluslicheniformis) wherein contained in microbial inoculum, and the growth rate of the Bacillus licheniformis in microbial inoculum is quickly, the miscellaneous bacteria in bedding and padding tails off gradually.This is owing to when > 55 DEG C, most der Pilz is killed, and additionally has many antibacterial miscellaneous bacteria also non-refractories, but the Bacillus licheniformis in microbial inoculum under the high temperature conditions can dormancy, when after the megathermal period, just become the main bacteria seed in bedding and padding.Can be seen that from the Data Comparison of embodiment 4-5 Yu embodiment 6, it is preferred that fermenting bed padding be sawdust, rice husk and wheat bran be used in conjunction with preparation fermentation bed when, the microbial inoculum bacterium in bedding and padding is more, and growth rate is also faster.
Testing example 4
This testing example measures the bedding and padding pre fermentation method effect for fermentation bed cultivation technology of embodiment 4-6 and comparative example 3-4.
Select identical age in days, Du × the length of body weight close (about 10kg) × Dasanyuan hybridization porkling 100, it is equally divided into 5 groups according to the principle that body weight and sex ratio are close, is respectively adopted the microbial bacterial agent identical with embodiment 4-6 and comparative example 3-4 and bedding and padding pre fermentation method prepares fermentation bed.
Above-mentioned 5 groups of porklings feed 70 days, test and add up the growth performance of pig house environment situation and pig, and result is as shown in table 6.
Table 6
| Group | Colony house situation | Pig growing state |
| Embodiment 4 | Without obvious stink | Hair color light, pig body is clean |
| Embodiment 5 | Without obvious stink | Hair color light, pig body is clean |
| Embodiment 6 | Without obvious stink | Hair color light, pig body is clean |
| Comparative example 3 | Stench | Dull, pig body is dirtier |
| Comparative example 4 | Frowziness | Dull, pig body is dirtier |
Data according to table 6, it can be seen that the colony house environment of embodiment 4-6 is substantially good than comparative example 3-4, illustrates that the microbial bacterial agent of the application present invention can eliminate cultivation stench efficiently, improve the environment of cultivation colony house, reduce stink to environmental emission.
Table 7
| Group | First weight/kg | End weight/kg | Daily gain/kg | Feedstuff-meat ratio |
| Embodiment 4 | 10.56±1.45 | 52.47±5.09 | 0.60±0.03 | 2.34 |
| Embodiment 5 | 10.37±1.53 | 51.67±4.15 | 0.59±0.04 | 2.38 |
| Embodiment 6 | 10.41±1.48 | 51.01±4.15 | 0.58±0.04 | 2.41 |
| Comparative example 3 | 10.44±1.22 | 44.57±4.23 | 0.49±0.04 | 2.88 |
| Comparative example 4 | 10.48±1.22 | 47.70±4.23 | 0.53±0.04 | 2.64 |
Data according to table 7 can be seen that, the cultivation pig that each side growth performances such as the daily gain and the feedstuff-meat ratio that cultivate pig in embodiment 4-6 are substantially better than in comparative example 3-4, this is after being applied to fermentation bed cultivation due to the microbial bacterial agent of the present invention, eliminate cultivation colony house foul smell, the growing environment making cultivation pig is greatly improved, promote the growth of cultivation pig, improve the benefit of cultivation.Can be seen that from the Data Comparison of embodiment 4-5 Yu embodiment 6, it is preferred that fermenting bed padding be sawdust, rice husk and wheat bran be used in conjunction with preparation fermentation bed when, cultivation pig growth performance more excellent.
The preferred embodiment of the present invention described in detail above; but, the present invention is not limited to the detail in above-mentioned embodiment, in the technology concept of the present invention; technical scheme can being carried out multiple simple variant, these simple variant belong to protection scope of the present invention.
It is further to note that, each concrete technical characteristic described in above-mentioned detailed description of the invention, in reconcilable situation, it is possible to be combined by any suitable mode, in order to avoid unnecessary repetition, various possible compound modes are no longer illustrated by the present invention separately.
Additionally, can also carry out combination in any between the various different embodiment of the present invention, as long as it is without prejudice to the thought of the present invention, it should be considered as content disclosed in this invention equally.
Claims (10)
1. a Bacillus licheniformis, it is characterised in that: the deposit number of this Bacillus licheniformis (Bacilluslicheniformis) is CGMCCNo.11235.
2. a microbial bacterial agent, this microbial bacterial agent contains thalline and culture medium, it is characterised in that: described thalline contains the Bacillus licheniformis that deposit number is CGMCCNo.11235.
3. microbial bacterial agent according to claim 2, it is characterised in that: in every gram of described microbial bacterial agent, deposit number is the viable count of the Bacillus licheniformis of CGMCCNo.11235 is 109-1010cfu。
4. the microbial bacterial agent according to Claims 2 or 3, it is characterised in that: described culture medium is at least one in beef-protein medium, nutrient broth medium and LB culture medium.
5. Bacillus licheniformis described in claim 1 and microbial bacterial agent described in any one application in fermentation bed cultivation in claim 2-4.
6. the application of 5 according to claim, it is characterised in that: described fermentation bed contains bedding and padding and described microbial bacterial agent, and wherein, the weight ratio of microbial bacterial agent and bedding and padding is 1:(900-1200).
7. application according to claim 6, it is characterised in that: the moisture content of described bedding and padding is 40-50 weight %.
8. the application according to claim 6 or 7, it is characterised in that: described bedding and padding are one or more in sawdust, rice husk and wheat bran.
9. application according to claim 8, it is characterised in that: relative to the sawdust of 100 weight portions, the consumption of rice husk is 60-70 weight portion, and the consumption of wheat bran is 5-10 weight portion.
10. application according to claim 5, it is characterised in that: described fermentation bed cultivation includes at least one in fermentation bed to raise pig, fermentation bed poultry, fermentation bed duck culturing and fermentation bed sheep raising.
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| CN201510886295.1A CN105754888B (en) | 2015-12-04 | 2015-12-04 | Bacillus licheniformis and microbial bacterial agent and their applications in fermentation bed cultivation |
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Cited By (5)
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| CN107502572A (en) * | 2017-09-08 | 2017-12-22 | 中国农业科学院农业环境与可持续发展研究所 | Application of the bacillus licheniformis in straw degradative, the microbial bacterial agent comprising the bacterium and its application |
| CN108330078A (en) * | 2017-08-14 | 2018-07-27 | 北京瓜尔润科技股份有限公司 | It is a kind of to be used to improve the lichengermium of crude protein yield and its application in melon bean pulp fermentation |
| CN110699299A (en) * | 2019-11-14 | 2020-01-17 | 乌拉特前旗荣生大地生物科技饲料有限责任公司 | Bacillus licheniformis X173 strain for producing urease inhibitor and application thereof |
| CN110964639A (en) * | 2019-12-09 | 2020-04-07 | 湖南金泥生物科技有限公司 | Strain screening method applied to pig raising fermentation bed and application |
| CN114806936A (en) * | 2022-04-14 | 2022-07-29 | 上海市农业科学院 | Bacillus licheniformis with antibacterial and elemental selenium synthesis capacity and application thereof |
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Cited By (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108330078A (en) * | 2017-08-14 | 2018-07-27 | 北京瓜尔润科技股份有限公司 | It is a kind of to be used to improve the lichengermium of crude protein yield and its application in melon bean pulp fermentation |
| CN108330078B (en) * | 2017-08-14 | 2021-10-08 | 北京瓜尔润科技股份有限公司 | Bacillus licheniformis for improving crude protein yield in guar meal fermentation and application thereof |
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| CN107502572B (en) * | 2017-09-08 | 2020-08-21 | 中国农业科学院农业环境与可持续发展研究所 | Application of bacillus licheniformis in straw degradation, microbial agent containing bacillus licheniformis and application of bacillus licheniformis |
| CN110699299A (en) * | 2019-11-14 | 2020-01-17 | 乌拉特前旗荣生大地生物科技饲料有限责任公司 | Bacillus licheniformis X173 strain for producing urease inhibitor and application thereof |
| CN110964639A (en) * | 2019-12-09 | 2020-04-07 | 湖南金泥生物科技有限公司 | Strain screening method applied to pig raising fermentation bed and application |
| CN114806936A (en) * | 2022-04-14 | 2022-07-29 | 上海市农业科学院 | Bacillus licheniformis with antibacterial and elemental selenium synthesis capacity and application thereof |
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