CN109370934B - Microbial agent for treating aquaculture sewage - Google Patents
Microbial agent for treating aquaculture sewage Download PDFInfo
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- CN109370934B CN109370934B CN201811198578.7A CN201811198578A CN109370934B CN 109370934 B CN109370934 B CN 109370934B CN 201811198578 A CN201811198578 A CN 201811198578A CN 109370934 B CN109370934 B CN 109370934B
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- 239000010865 sewage Substances 0.000 title claims abstract description 31
- 238000009360 aquaculture Methods 0.000 title claims abstract description 22
- 244000144974 aquaculture Species 0.000 title claims abstract description 22
- 230000000813 microbial effect Effects 0.000 title claims abstract description 18
- 241000894006 Bacteria Species 0.000 claims abstract description 34
- 230000000593 degrading effect Effects 0.000 claims abstract description 22
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims abstract description 21
- OAICVXFJPJFONN-UHFFFAOYSA-N Phosphorus Chemical compound [P] OAICVXFJPJFONN-UHFFFAOYSA-N 0.000 claims abstract description 17
- XKMRRTOUMJRJIA-UHFFFAOYSA-N ammonia nh3 Chemical compound N.N XKMRRTOUMJRJIA-UHFFFAOYSA-N 0.000 claims abstract description 17
- 239000003795 chemical substances by application Substances 0.000 claims abstract description 17
- 239000011574 phosphorus Substances 0.000 claims abstract description 17
- 229910052698 phosphorus Inorganic materials 0.000 claims abstract description 17
- 238000000855 fermentation Methods 0.000 claims description 57
- 230000004151 fermentation Effects 0.000 claims description 57
- FAPWRFPIFSIZLT-UHFFFAOYSA-M Sodium chloride Chemical compound [Na+].[Cl-] FAPWRFPIFSIZLT-UHFFFAOYSA-M 0.000 claims description 48
- 239000001963 growth medium Substances 0.000 claims description 43
- 238000009423 ventilation Methods 0.000 claims description 30
- 238000002360 preparation method Methods 0.000 claims description 25
- 239000011780 sodium chloride Substances 0.000 claims description 24
- 238000003756 stirring Methods 0.000 claims description 21
- 239000001888 Peptone Substances 0.000 claims description 18
- 108010080698 Peptones Proteins 0.000 claims description 18
- 235000019319 peptone Nutrition 0.000 claims description 18
- 235000013312 flour Nutrition 0.000 claims description 15
- 229910000402 monopotassium phosphate Inorganic materials 0.000 claims description 14
- 241000193398 Bacillus methanolicus Species 0.000 claims description 13
- 241000589615 Pseudomonas syringae Species 0.000 claims description 13
- 241000190950 Rhodopseudomonas palustris Species 0.000 claims description 13
- 229930006000 Sucrose Natural products 0.000 claims description 12
- CZMRCDWAGMRECN-UGDNZRGBSA-N Sucrose Chemical compound O[C@H]1[C@H](O)[C@@H](CO)O[C@@]1(CO)O[C@@H]1[C@H](O)[C@@H](O)[C@H](O)[C@@H](CO)O1 CZMRCDWAGMRECN-UGDNZRGBSA-N 0.000 claims description 12
- QVGXLLKOCUKJST-UHFFFAOYSA-N atomic oxygen Chemical compound [O] QVGXLLKOCUKJST-UHFFFAOYSA-N 0.000 claims description 12
- 235000019796 monopotassium phosphate Nutrition 0.000 claims description 12
- 229910052760 oxygen Inorganic materials 0.000 claims description 12
- 239000001301 oxygen Substances 0.000 claims description 12
- 229960004793 sucrose Drugs 0.000 claims description 12
- CSNNHWWHGAXBCP-UHFFFAOYSA-L magnesium sulphate Substances [Mg+2].[O-][S+2]([O-])([O-])[O-] CSNNHWWHGAXBCP-UHFFFAOYSA-L 0.000 claims description 11
- 241000193744 Bacillus amyloliquefaciens Species 0.000 claims description 10
- 241000193755 Bacillus cereus Species 0.000 claims description 10
- 244000063299 Bacillus subtilis Species 0.000 claims description 10
- 235000014469 Bacillus subtilis Nutrition 0.000 claims description 10
- UXVMQQNJUSDDNG-UHFFFAOYSA-L Calcium chloride Chemical compound [Cl-].[Cl-].[Ca+2] UXVMQQNJUSDDNG-UHFFFAOYSA-L 0.000 claims description 10
- 241000589516 Pseudomonas Species 0.000 claims description 10
- 229910052921 ammonium sulfate Inorganic materials 0.000 claims description 10
- 239000001110 calcium chloride Substances 0.000 claims description 10
- 229910001628 calcium chloride Inorganic materials 0.000 claims description 10
- 238000000034 method Methods 0.000 claims description 10
- WQZGKKKJIJFFOK-GASJEMHNSA-N Glucose Natural products OC[C@H]1OC(O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-GASJEMHNSA-N 0.000 claims description 9
- 244000046052 Phaseolus vulgaris Species 0.000 claims description 9
- 235000010627 Phaseolus vulgaris Nutrition 0.000 claims description 9
- 240000008042 Zea mays Species 0.000 claims description 9
- 235000005824 Zea mays ssp. parviglumis Nutrition 0.000 claims description 9
- 235000002017 Zea mays subsp mays Nutrition 0.000 claims description 9
- BFNBIHQBYMNNAN-UHFFFAOYSA-N ammonium sulfate Chemical compound N.N.OS(O)(=O)=O BFNBIHQBYMNNAN-UHFFFAOYSA-N 0.000 claims description 9
- 235000011130 ammonium sulphate Nutrition 0.000 claims description 9
- 235000015278 beef Nutrition 0.000 claims description 9
- 235000005822 corn Nutrition 0.000 claims description 9
- 239000000284 extract Substances 0.000 claims description 9
- 239000008103 glucose Substances 0.000 claims description 9
- 239000000843 powder Substances 0.000 claims description 9
- 239000002518 antifoaming agent Substances 0.000 claims description 8
- 241000222175 Diutina rugosa Species 0.000 claims description 7
- 241000235645 Pichia kudriavzevii Species 0.000 claims description 7
- PJNZPQUBCPKICU-UHFFFAOYSA-N phosphoric acid;potassium Chemical compound [K].OP(O)(O)=O PJNZPQUBCPKICU-UHFFFAOYSA-N 0.000 claims description 7
- GNSKLFRGEWLPPA-UHFFFAOYSA-M potassium dihydrogen phosphate Chemical compound [K+].OP(O)([O-])=O GNSKLFRGEWLPPA-UHFFFAOYSA-M 0.000 claims description 7
- NLXLAEXVIDQMFP-UHFFFAOYSA-N Ammonia chloride Chemical compound [NH4+].[Cl-] NLXLAEXVIDQMFP-UHFFFAOYSA-N 0.000 claims description 6
- 241000222120 Candida <Saccharomycetales> Species 0.000 claims description 6
- 240000004808 Saccharomyces cerevisiae Species 0.000 claims description 6
- 238000005273 aeration Methods 0.000 claims description 6
- 229910052943 magnesium sulfate Inorganic materials 0.000 claims description 6
- 235000019341 magnesium sulphate Nutrition 0.000 claims description 5
- LWIHDJKSTIGBAC-UHFFFAOYSA-K potassium phosphate Substances [K+].[K+].[K+].[O-]P([O-])([O-])=O LWIHDJKSTIGBAC-UHFFFAOYSA-K 0.000 claims description 5
- 239000002068 microbial inoculum Substances 0.000 claims description 4
- 239000004382 Amylase Substances 0.000 claims description 3
- 102000013142 Amylases Human genes 0.000 claims description 3
- 108010065511 Amylases Proteins 0.000 claims description 3
- 108091005804 Peptidases Proteins 0.000 claims description 3
- 239000004365 Protease Substances 0.000 claims description 3
- 102100037486 Reverse transcriptase/ribonuclease H Human genes 0.000 claims description 3
- 235000019764 Soybean Meal Nutrition 0.000 claims description 3
- 241000122971 Stenotrophomonas Species 0.000 claims description 3
- 235000019270 ammonium chloride Nutrition 0.000 claims description 3
- 235000019418 amylase Nutrition 0.000 claims description 3
- 229940041514 candida albicans extract Drugs 0.000 claims description 3
- 238000011081 inoculation Methods 0.000 claims description 3
- 239000004455 soybean meal Substances 0.000 claims description 3
- 239000005720 sucrose Substances 0.000 claims description 3
- 239000012137 tryptone Substances 0.000 claims description 3
- 239000012138 yeast extract Substances 0.000 claims description 3
- 239000008213 purified water Substances 0.000 claims description 2
- 239000008399 tap water Substances 0.000 claims description 2
- 235000020679 tap water Nutrition 0.000 claims description 2
- 239000007836 KH2PO4 Substances 0.000 claims 2
- UIIMBOGNXHQVGW-UHFFFAOYSA-M Sodium bicarbonate Chemical compound [Na+].OC([O-])=O UIIMBOGNXHQVGW-UHFFFAOYSA-M 0.000 claims 2
- WQZGKKKJIJFFOK-VFUOTHLCSA-N beta-D-glucose Chemical compound OC[C@H]1O[C@@H](O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-VFUOTHLCSA-N 0.000 claims 1
- 239000013530 defoamer Substances 0.000 claims 1
- 229910052564 epsomite Inorganic materials 0.000 claims 1
- 238000002156 mixing Methods 0.000 claims 1
- 229910000030 sodium bicarbonate Inorganic materials 0.000 claims 1
- 150000001875 compounds Chemical class 0.000 abstract description 5
- 230000006378 damage Effects 0.000 abstract description 4
- 239000002054 inoculum Substances 0.000 abstract 1
- 238000009395 breeding Methods 0.000 description 9
- 230000001488 breeding effect Effects 0.000 description 9
- 230000015556 catabolic process Effects 0.000 description 9
- 238000006731 degradation reaction Methods 0.000 description 9
- 244000144972 livestock Species 0.000 description 8
- 230000000694 effects Effects 0.000 description 7
- 244000144977 poultry Species 0.000 description 7
- 230000001580 bacterial effect Effects 0.000 description 5
- 239000002351 wastewater Substances 0.000 description 5
- 230000009286 beneficial effect Effects 0.000 description 3
- 230000007547 defect Effects 0.000 description 3
- 238000013461 design Methods 0.000 description 3
- 238000011161 development Methods 0.000 description 3
- 238000005516 engineering process Methods 0.000 description 3
- 238000002474 experimental method Methods 0.000 description 3
- 239000010802 sludge Substances 0.000 description 3
- 239000000126 substance Substances 0.000 description 3
- IJGRMHOSHXDMSA-UHFFFAOYSA-N Atomic nitrogen Chemical compound N#N IJGRMHOSHXDMSA-UHFFFAOYSA-N 0.000 description 2
- VMHLLURERBWHNL-UHFFFAOYSA-M Sodium acetate Chemical compound [Na+].CC([O-])=O VMHLLURERBWHNL-UHFFFAOYSA-M 0.000 description 2
- 244000052616 bacterial pathogen Species 0.000 description 2
- 239000002131 composite material Substances 0.000 description 2
- 238000012216 screening Methods 0.000 description 2
- 235000017281 sodium acetate Nutrition 0.000 description 2
- 238000003911 water pollution Methods 0.000 description 2
- 241001465754 Metazoa Species 0.000 description 1
- IOVCWXUNBOPUCH-UHFFFAOYSA-M Nitrite anion Chemical compound [O-]N=O IOVCWXUNBOPUCH-UHFFFAOYSA-M 0.000 description 1
- JVMRPSJZNHXORP-UHFFFAOYSA-N ON=O.ON=O.ON=O.N Chemical compound ON=O.ON=O.ON=O.N JVMRPSJZNHXORP-UHFFFAOYSA-N 0.000 description 1
- ZGSDJMADBJCNPN-UHFFFAOYSA-N [S-][NH3+] Chemical compound [S-][NH3+] ZGSDJMADBJCNPN-UHFFFAOYSA-N 0.000 description 1
- 238000009825 accumulation Methods 0.000 description 1
- 239000003242 anti bacterial agent Substances 0.000 description 1
- 229940088710 antibiotic agent Drugs 0.000 description 1
- 230000003115 biocidal effect Effects 0.000 description 1
- 238000004364 calculation method Methods 0.000 description 1
- 238000004140 cleaning Methods 0.000 description 1
- 230000006866 deterioration Effects 0.000 description 1
- 239000003814 drug Substances 0.000 description 1
- 229940079593 drug Drugs 0.000 description 1
- 230000007613 environmental effect Effects 0.000 description 1
- 239000003344 environmental pollutant Substances 0.000 description 1
- 238000012851 eutrophication Methods 0.000 description 1
- 210000003608 fece Anatomy 0.000 description 1
- 229910001385 heavy metal Inorganic materials 0.000 description 1
- 239000005556 hormone Substances 0.000 description 1
- 229940088597 hormone Drugs 0.000 description 1
- 239000008235 industrial water Substances 0.000 description 1
- 239000003112 inhibitor Substances 0.000 description 1
- 238000003973 irrigation Methods 0.000 description 1
- 230000002262 irrigation Effects 0.000 description 1
- 239000010871 livestock manure Substances 0.000 description 1
- 238000004519 manufacturing process Methods 0.000 description 1
- 238000005259 measurement Methods 0.000 description 1
- 239000012528 membrane Substances 0.000 description 1
- 230000037353 metabolic pathway Effects 0.000 description 1
- 244000005700 microbiome Species 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 229910052757 nitrogen Inorganic materials 0.000 description 1
- 230000000050 nutritive effect Effects 0.000 description 1
- 230000003071 parasitic effect Effects 0.000 description 1
- 231100000719 pollutant Toxicity 0.000 description 1
- 230000002265 prevention Effects 0.000 description 1
- 230000001737 promoting effect Effects 0.000 description 1
- 150000003839 salts Chemical class 0.000 description 1
- 238000005070 sampling Methods 0.000 description 1
- 239000002002 slurry Substances 0.000 description 1
- 239000002910 solid waste Substances 0.000 description 1
- 208000024891 symptom Diseases 0.000 description 1
- 238000012360 testing method Methods 0.000 description 1
- 239000002699 waste material Substances 0.000 description 1
- 238000004065 wastewater treatment Methods 0.000 description 1
Classifications
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/20—Bacteria; Culture media therefor
-
- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F3/00—Biological treatment of water, waste water, or sewage
- C02F3/34—Biological treatment of water, waste water, or sewage characterised by the microorganisms used
-
- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F2103/00—Nature of the water, waste water, sewage or sludge to be treated
- C02F2103/20—Nature of the water, waste water, sewage or sludge to be treated from animal husbandry
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- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Engineering & Computer Science (AREA)
- Microbiology (AREA)
- Organic Chemistry (AREA)
- Health & Medical Sciences (AREA)
- Biotechnology (AREA)
- Zoology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Wood Science & Technology (AREA)
- Genetics & Genomics (AREA)
- Tropical Medicine & Parasitology (AREA)
- Biomedical Technology (AREA)
- Virology (AREA)
- Biochemistry (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Medicinal Chemistry (AREA)
- Biodiversity & Conservation Biology (AREA)
- Hydrology & Water Resources (AREA)
- Environmental & Geological Engineering (AREA)
- Water Supply & Treatment (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Purification Treatments By Anaerobic Or Anaerobic And Aerobic Bacteria Or Animals (AREA)
Abstract
The invention discloses a microbial agent for treating aquaculture sewage, which comprises ammonia nitrogen degrading bacteria, COD degrading bacteria and phosphorus removing bacteria, wherein the ammonia nitrogen degrading bacteria, the COD degrading bacteria, the phosphorus removing bacteria and a proper amount of water are mixed to prepare a finished product of a sewage treatment microbial agent; can effectively handle breed sewage, reduce the operation expense, let the treated water discharge to reach standard, reduce the destruction to the environment, compound multiple function microbial inoculant improves and breeds quality of water and has positive meaning to agricultural products cultivation technical field, and the practicality is excellent, is a fine innovation scheme, has very much the marketing prospect.
Description
Technical Field
The invention relates to a sewage treatment technology, in particular to a microbial agent for treating aquaculture sewage.
Background
With the rapid development of the aquaculture industry, particularly intensive aquaculture causes serious water pollution, in recent reports, the problem of water quality deterioration caused by aquaculture is more and more. The main pollutants in the culture water area are feed residues, excrement and the use of some medicines, the accumulation of nitrogen and phosphorus nutritive salts in the feed can cause water eutrophication, the serious standard exceeding of chemical oxygen demand, ammonia nitrogen, nitrite and the like in the water can be caused, and the surrounding environment can be influenced by direct discharge. By 2015, the total livestock and poultry breeding amount in the country is about 14 hundred million (pig equivalent); according to the measurement and calculation of the pollution prevention level of the existing livestock and poultry breeding, the chemical oxygen demand and the annual emission amount of ammonia nitrogen are estimated to reach 1260 ten thousand tons and 80 ten thousand tons respectively, and the livestock breeding pollution is further aggravated due to the increase of large-scale breeding. The regulations on pollution control of livestock and poultry scale cultivation executed in 2014 and the action plans of water pollution control executed in 2015 put higher requirements on the cultivation sewage. The breeding waste sewage is required to be discharged after reaching the standard and the solid waste is recycled, so that the sustainable development of the breeding industry is realized.
In recent years, all parts of the country, related agricultural departments, though actively fulfilling the national regulations, advocate the farmers to use the dry manure cleaning process by means of measures such as popularization of the science of cultivation technology, and the like, the measures play a certain role in promoting the wastewater treatment of small-scale cultivation farms, but have a large wastewater discharge amount and little effect on large and medium-scale cultivation farms. Because the livestock and poultry breeding wastewater has high concentration and contains a large amount of various hormones such as pathogenic bacteria, parasitic ova, antibiotics and the like and inhibitors such as heavy metals and the like, the livestock and poultry breeding wastewater cannot be directly discharged or used for farmland irrigation, and if the livestock and poultry breeding wastewater is directly discharged, the environment is seriously polluted, so a series of environmental problems are caused. The conventional industrial water treatment method is too high in treatment cost, and the problems of large biogas slurry discharge amount, incapability of being consumed and the like exist in the treatment of the conventional wet-type fermentation biogas engineering technology, so that the treatment of the antibiotic-containing culture wastewater is still a troublesome problem of pollution control in the livestock and poultry industry at present; the activated sludge method has large sludge production amount and difficult sludge control; the MBR biofilm method has the defects of easy membrane blockage, high operation cost and the like, and cannot meet the requirement of high-speed social development.
In summary, in order to overcome the defects of the prior art, a microbial agent for treating aquaculture sewage and a preparation method thereof are particularly needed.
Disclosure of Invention
Aiming at the problems in the prior art and influencing the practical use, the invention provides a microbial agent for treating aquaculture sewage, which is novel in design, can effectively treat the aquaculture sewage, reduces the operation cost, enables the treated water to reach the standard and discharge, reduces the damage to the environment, and overcomes the defects in the prior art.
In order to achieve the purpose, the technical scheme of the invention is as follows:
a microbial agent for treating culture sewage comprises ammonia nitrogen degrading bacteria, COD degrading bacteria and phosphorus removing bacteria, wherein the ammonia nitrogen degrading bacteria, the COD degrading bacteria, the phosphorus removing bacteria and a proper amount of water are mixed to prepare a finished product of the microbial agent for treating the culture sewage;
the ammonia nitrogen degrading bacteria comprise bacillus subtilis, bacillus cereus, bacillus amyloliquefaciens, candida krusei and candida rugosa;
the COD degrading bacteria comprise Bacillus methanolicus and Rhodopseudomonas palustris;
the phosphorus-removing bacteria include Pseudomonas syringae and Pseudomonas oligooxygen.
Further, the bacillus subtilis is 5.0 multiplied by 109cfu/ml, Bacillus cereus 1.0X 109cfu/ml, Bacillus amyloliquefaciens 1.5X 109cfu/ml, Candida krusei 5.0 × 109cfu/ml, Candida rugosa 5.0X 109cfu/ml, Bacillus methanolicus 1.2X 109cfu/ml, Rhodopseudomonas palustris 1.5X 109cfu/ml, Pseudomonas syringae 1.5X 109cfu/ml, Pseudomonas oligooxygen 1.0X 109cfu/ml。
Further, the water is selected from tap water or purified water.
Further, the invention:
the preparation method of the bacillus subtilis comprises the following steps:
the culture medium is as follows:
10kg/T of cane sugar, 0.05kg/T of glucose, 8kg/T of bean powder, 5kg/T of peptone, 1kg/T of beef extract, 1kg/T of ammonium sulfate and 5kg/T of sodium chloride;
the fermentation conditions of the culture medium are as follows: at the temperature of 37 ℃, the pH value is 7.0, the tank pressure is 1Kg, the ventilation volume is 80 cubic/h, the stirring speed is 80rpm, the dissolved oxygen is 30 percent, and the fermentation time is about 24 hours;
the preparation method of the bacillus cereus comprises the following steps:
the culture medium is as follows:
10kg/T of cane sugar, 8kg/T of bean flour, 5kg/T of peptone, 1kg/T of beef extract, 3kg/T of sodium chloride, 1kg/T of ammonium sulfate and 2kg/T of monopotassium phosphate;
the fermentation conditions of the culture medium are as follows: at the temperature of 37 ℃, the pH value is 7.0, the tank pressure is 1Kg, the ventilation volume is 80 cubic/h, the stirring speed is 80rpm, the dissolved oxygen is 30 percent, and the fermentation time is about 24 hours;
the preparation method of the bacillus amyloliquefaciens comprises the following steps:
the culture medium is as follows:
10Kg/T of cane sugar, 6g/T of bean flour, 5Kg/T of peptone, 1Kg/T of beef extract, 5Kg/T of sodium chloride, pH7.0 and 2Kg/T of antifoaming agent;
the fermentation conditions of the culture medium are as follows: inoculating the shake flask seeds into a fermentation tank according to the inoculation amount of 1%, wherein the fermentation parameters of the fermentation tank (5 tons) are 37 ℃, the pH value is 7.0, the tank pressure is 1Kg, the ventilation volume is 80 cubic/h, the stirring is 80rpm, and the dissolved oxygen fermentation time is 30% for about 24 hours;
the preparation method of the krusei candida and the crinkliniella candida comprises the following steps:
the culture medium is as follows:
10kg/T of peptone, 8kg/T of sucrose, 2kg/T of glucose, KH2PO 41 kg/T, 0.5kg/T of MgSO4 & 7H2O 0.5 and 5kg/T of NaCl;
the fermentation conditions of the culture medium are as follows: 37 ℃, pH 7.0-7.2, tank pressure 0.05MPa, ventilation volume 10-15 cubic/h and fermentation time about 24 hours.
Further, the invention:
the preparation method of the bacillus methanolicus comprises the following steps:
the formula of the culture medium is as follows:
peptone 1%, yeast powder 0.5%, corn flour 0.5%, sodium chloride 0.1%, potassium dihydrogen phosphate 0.05%, ammonium chloride 0.025%, calcium chloride 0.05% (separately dissolved), antifoaming agent 0.05%, natural pH;
the fermentation process comprises the following steps: the culture temperature is as follows: 35 ℃, initial rotation speed: 80r/min, the ventilation volume is about 100L/h, the stirring can be stopped after 6-8 hours, the ventilation volume is increased to about 110L/h, and the fermentation period is 16-20 hours;
the preparation method of the rhodopseudomonas palustris comprises the following steps:
the formula of the culture medium is as follows:
the formula of the culture medium is as follows: CH3COONa 3g/L, NaHCO 35 g/L, (NH4)2SO 41 g/L, peptone 8g/L, yeast extract 1g/L, MgSO40.5g/L, NaCl 2g/L, KH2PO 41 g/L, CaCl 20.5g/L, pH 7.2;
the fermentation process comprises the following steps: temperature: 30 ℃, initial rotation speed: 100r/min, ventilation capacity of about 100L/H, stirring at 150r/min after 6-8 hours, keeping the ventilation capacity unchanged, and putting into a tank after 18-20 hours.
Further, the invention:
the preparation method of the pseudomonas syringae comprises the following steps:
the formula of the culture medium is as follows:
10g/L tryptone, 10ml/L glucose, 0.5% corn flour, 1g/L sodium chloride, 0.8g/L potassium dihydrogen phosphate, 1.5g/L magnesium sulfate, 1g/L calcium chloride (separately dissolved), natural pH;
the fermentation process comprises the following steps: the culture temperature is as follows: 30 ℃, initial rotation speed: 100r/min, the ventilation capacity is about 120L/h, stirring is carried out at 150r/min after 6-8 hours, the ventilation capacity is unchanged, and the fermentation period is 32 hours;
the preparation method of the stenotrophomonas comprises the following steps:
the formula of the culture medium is as follows:
the formula of the culture medium is as follows: 1% of soybean meal, 1% of corn flour, 0.3% of yeast powder, 0.2% of sodium chloride, 0.05% of potassium dihydrogen phosphate, 0.025% of ammonium sulfate, 0.05% of calcium chloride (dissolved independently), 0.05% of defoaming agent, a proper amount of protease and amylase and natural pH;
the fermentation process comprises the following steps: the culture temperature is as follows: 35 ℃, initial rotation speed: the aeration rate is 100L/H at 80r/min, the stirring can be stopped after 6-8 hours, the aeration rate is increased to about 110L/H, and the fermentation period is 16-20 hours.
The invention has the beneficial effects that: the novel design can effectively treat the aquaculture sewage, reduces the operation cost, enables the treated water to reach the standard and be discharged, reduces the damage to the environment, and the compound multifunctional microbial inoculum improves the aquaculture water quality, has positive significance to the technical field of agricultural product aquaculture, has excellent practical performance, is a good innovation scheme, and has great market popularization prospect.
Detailed Description
The present invention is further described in order to make the technical means, the creation features, the achievement purposes and the effects of the present invention easy to understand.
A microbial agent for treating culture sewage comprises ammonia nitrogen degrading bacteria, COD degrading bacteria and phosphorus removing bacteria, wherein the ammonia nitrogen degrading bacteria, the COD degrading bacteria, the phosphorus removing bacteria and a proper amount of water are mixed to prepare a finished product of the microbial agent for treating the culture sewage;
the ammonia nitrogen degrading bacteria comprise bacillus subtilis, bacillus cereus, bacillus amyloliquefaciens, candida krusei and candida rugosa;
the COD degrading bacteria comprise Bacillus methanolicus and Rhodopseudomonas palustris;
the phosphorus-removing bacteria include Pseudomonas syringae and Pseudomonas oligooxygen.
The preparation method of the bacillus subtilis comprises the following steps:
the culture medium is as follows:
10kg/T of cane sugar, 0.05kg/T of glucose, 8kg/T of bean powder, 5kg/T of peptone, 1kg/T of beef extract, 1kg/T of ammonium sulfate and 5kg/T of sodium chloride;
the fermentation conditions of the culture medium are as follows: at the temperature of 37 ℃, the pH value is 7.0, the tank pressure is 1Kg, the ventilation volume is 80 cubic/h, the stirring speed is 80rpm, the dissolved oxygen is 30 percent, and the fermentation time is about 24 hours;
the preparation method of the bacillus cereus comprises the following steps:
the culture medium is as follows:
10kg/T of cane sugar, 8kg/T of bean flour, 5kg/T of peptone, 1kg/T of beef extract, 3kg/T of sodium chloride, 1kg/T of ammonium sulfate and 2kg/T of monopotassium phosphate;
the fermentation conditions of the culture medium are as follows: at the temperature of 37 ℃, the pH value is 7.0, the tank pressure is 1Kg, the ventilation volume is 80 cubic/h, the stirring speed is 80rpm, the dissolved oxygen is 30 percent, and the fermentation time is about 24 hours;
the preparation method of the bacillus amyloliquefaciens comprises the following steps:
the culture medium is as follows:
10Kg/T of cane sugar, 6g/T of bean flour, 5Kg/T of peptone, 1Kg/T of beef extract, 5Kg/T of sodium chloride, pH7.0 and 2Kg/T of antifoaming agent;
the fermentation conditions of the culture medium are as follows: inoculating the shake flask seeds into a fermentation tank according to the inoculation amount of 1%, wherein the fermentation parameters of the fermentation tank (5 tons) are 37 ℃, the pH value is 7.0, the tank pressure is 1Kg, the ventilation volume is 80 cubic/h, the stirring is 80rpm, and the dissolved oxygen fermentation time is 30% for about 24 hours;
the preparation method of the krusei candida and the crinkliniella candida comprises the following steps:
the culture medium is as follows:
10kg/T of peptone, 8kg/T of sucrose, 2kg/T of glucose, KH2PO 41 kg/T, 0.5kg/T of MgSO4 & 7H2O 0.5 and 5kg/T of NaCl;
the fermentation conditions of the culture medium are as follows: 37 ℃, pH 7.0-7.2, tank pressure 0.05MPa, ventilation volume 10-15 cubic/h and fermentation time about 24 hours.
The preparation method of the bacillus methanolicus comprises the following steps:
the formula of the culture medium is as follows:
peptone 1%, yeast powder 0.5%, corn flour 0.5%, sodium chloride 0.1%, potassium dihydrogen phosphate 0.05%, ammonium chloride 0.025%, calcium chloride 0.05% (separately dissolved), antifoaming agent 0.05%, natural pH;
the fermentation process comprises the following steps: the culture temperature is as follows: 35 ℃, initial rotation speed: 80r/min, the ventilation volume is about 100L/h, the stirring can be stopped after 6-8 hours, the ventilation volume is increased to about 110L/h, and the fermentation period is 16-20 hours;
the preparation method of the rhodopseudomonas palustris comprises the following steps:
the formula of the culture medium is as follows:
the formula of the culture medium is as follows: CH3COONa 3g/L, NaHCO 35 g/L, (NH4)2SO 41 g/L, peptone 8g/L, yeast extract 1g/L, MgSO40.5g/L, NaCl 2g/L, KH2PO 41 g/L, CaCl 20.5g/L, pH 7.2;
the fermentation process comprises the following steps: temperature: 30 ℃, initial rotation speed: 100r/min, ventilation capacity of about 100L/H, stirring at 150r/min after 6-8 hours, keeping the ventilation capacity unchanged, and putting into a tank after 18-20 hours.
The preparation method of the pseudomonas syringae comprises the following steps:
the formula of the culture medium is as follows:
10g/L tryptone, 10ml/L glucose, 0.5% corn flour, 1g/L sodium chloride, 0.8g/L potassium dihydrogen phosphate, 1.5g/L magnesium sulfate, 1g/L calcium chloride (separately dissolved), natural pH;
the fermentation process comprises the following steps: the culture temperature is as follows: 30 ℃, initial rotation speed: 100r/min, the ventilation capacity is about 120L/h, stirring is carried out at 150r/min after 6-8 hours, the ventilation capacity is unchanged, and the fermentation period is 32 hours;
the preparation method of the stenotrophomonas comprises the following steps:
the formula of the culture medium is as follows:
the formula of the culture medium is as follows: 1% of soybean meal, 1% of corn flour, 0.3% of yeast powder, 0.2% of sodium chloride, 0.05% of potassium dihydrogen phosphate, 0.025% of ammonium sulfate, 0.05% of calcium chloride (dissolved independently), 0.05% of defoaming agent, a proper amount of protease and amylase and natural pH;
the fermentation process comprises the following steps: the culture temperature is as follows: 35 ℃, initial rotation speed: the aeration rate is 100L/H at 80r/min, the stirring can be stopped after 6-8 hours, the aeration rate is increased to about 110L/H, and the fermentation period is 16-20 hours.
Example 1:
an efficient sewage treating bacterial agent is prepared from bacillus subtilis (5.0X 10)9cfu/ml, Bacillus cereus 1.0X 109cfu/ml, Bacillus amyloliquefaciens 1.5X 109cfu/ml, Candida krusei 5.0 × 109cfu/ml, Candida rugosa 5.0X 109cfu/ml, Bacillus methanolicus 1.2X 109cfu/ml, Rhodopseudomonas palustris 1.5X 109cfu/ml, Pseudomonas syringae 1.5X 109cfu/ml, Pseudomonas oligooxygen 1.0X 109cfu/ml of Bacillus subtilis is 5.0 × 109cfu/ml, Bacillus cereus 1.0X 109cfu/ml, Bacillus amyloliquefaciens 1.5X 109cfu/ml, Candida krusei 5.0 × 109cfu/ml, Candida rugosa 5.0X 109cfu/ml, Bacillus methanolicus 1.2X 109cfu/ml, Rhodopseudomonas palustris 1.5X 109cfu/ml, Pseudomonas syringae 1.5X 109cfu/ml, Pseudomonas oligooxygen 1.0X 109cfu/ml and water. After treatment, COD is less than or equal to 150mg/ml, ammonia nitrogen is less than or equal to 70mg/ml, and total phosphorus is less than or equal to 7 mg/ml.
The addition amount is 2%, and the micro-aquatic microbial inoculum is supplemented once every 6 months.
The bacillus methanolicus and the rhodopseudomonas palustris are mixed, the COD degradation rate reaches 70% in 5 days, and reaches 95% in 7 days.
The pseudomonas syringae and the pseudomonas oligooxyens are mixed, the phosphorus removal rate reaches 75% in 5 days, and 90% in 7 days.
Example 2:
COD degradation experiment
And (3) shaking the fermentation culture medium of the bacillus methanolicus and the rhodopseudomonas palustris, and then determining the number of colonies to ensure that the number of the colonies is consistent. The composite strain is prepared according to the proportion of 1:1, and the quantity of the composite strain is the same as that of a single strain. Adjusting sewage components, preparing COD2000mg/ml and ammonia nitrogen 1000mg/ml by compounds according to a proportion, and aerating at regular time according to a treatment process. Sampling is carried out periodically to determine COD. And analyzing and comparing the COD degradation rates of the bacterial strains in different batches, and screening to obtain the bacterial strain with the highest degradation rate. The results are shown in Table 1.
TABLE 1
The bacillus methanolicus and the rhodopseudomonas palustris both have COD (chemical oxygen demand) degradation effects, the mixed use effect of the two bacteria is better, the degradation rate of 5d reaches 70%, and the degradation rate of 7d reaches 95%.
Example 3:
phosphorus removal experiment
The pseudomonas syringae and the pseudomonas oligooxyens are connected into a sewage environment, different sewage treatment environments are simulated, and the phosphorus removal effect is verified through repeated experiments. Adjusting sewage components, preparing COD2000mg/ml, ammonia nitrogen 1000mg/ml and total phosphorus 100mg/ml by using compounds according to a proportion, and aerating at regular time according to a treatment process. Samples were taken periodically to determine the total phosphorus. And (4) analyzing and comparing the total phosphorus removal rate of the bacterial strain sewage of different batches of tests, and screening to obtain the bacterial strain with the highest removal rate. The results are shown in Table 2;
TABLE 2
The pseudomonas syringae GP604 and the pseudomonas oligotrophic GP201 can both remove total phosphorus, the mixed use effect of the two strains is better, the degradation rate at 5d reaches 75%, and the degradation rate at 7d reaches 90%.
The metabolic pathway of beneficial microorganisms in the growth and propagation process of water bodies is utilized to decompose organic matters, absorb ammonia nitrogen, nitrite nitrogen, ammonia sulfide and the like, macromolecular organic matters are effectively decomposed, the mass propagation of pathogenic bacteria of aquatic animals is inhibited, the action effect is durable, and the method is a treatment method for treating both symptoms and root causes.
The invention has the beneficial effects that: the novel design can effectively treat the aquaculture sewage, reduces the operation cost, enables the treated water to reach the standard and be discharged, reduces the damage to the environment, and the compound multifunctional microbial inoculum improves the aquaculture water quality, has positive significance to the technical field of agricultural product aquaculture, has excellent practical performance, is a good innovation scheme, and has great market popularization prospect.
It will be understood by those skilled in the art that the present invention is not limited to the embodiments described above, which are given by way of illustration of the principles of the present invention, and that various changes and modifications may be made without departing from the spirit and scope of the invention as defined by the appended claims. The scope of the invention is defined by the appended claims and equivalents thereof.
Claims (6)
1. A microbial agent for treating aquaculture sewage is characterized in that: the method comprises the steps of mixing ammonia nitrogen degrading bacteria, COD degrading bacteria, phosphorus removing bacteria, ammonia nitrogen degrading bacteria, COD degrading bacteria, phosphorus removing bacteria and a proper amount of water to prepare a finished product of the sewage treatment microbial inoculum;
the ammonia nitrogen degrading bacteria comprise bacillus subtilis, bacillus cereus, bacillus amyloliquefaciens, candida krusei and candida rugosa;
the COD degrading bacteria comprise Bacillus methanolicus and Rhodopseudomonas palustris;
the phosphorus-removing bacteria include Pseudomonas syringae and Pseudomonas oligooxygen.
2. The microbial agent for treating aquaculture sewage according to claim 1, which is characterized in that: the bacillus subtilis is 5.0 multiplied by 109cfu/ml, Bacillus cereus 1.0X 109cfu/ml, Bacillus amyloliquefaciens 1.5X 109cfu/ml, Candida krusei 5.0 × 109cfu/ml, Candida rugosa 5.0X 109cfu/ml, Bacillus methanolicus 1.2X 109cfu/ml, Rhodopseudomonas palustris 1.5X 109cfu/ml, Pseudomonas syringae 1.5X 109cfu/ml, Pseudomonas oligooxygen 1.0X 109cfu/ml。
3. The microbial agent for treating aquaculture sewage according to claim 1, which is characterized in that: the water is tap water or purified water.
4. The microbial agent for treating aquaculture sewage according to claim 1, which is characterized in that:
the preparation method of the bacillus subtilis comprises the following steps:
the culture medium is as follows:
10kg/T of cane sugar, 0.05kg/T of glucose, 8kg/T of bean powder, 5kg/T of peptone, 1kg/T of beef extract, 1kg/T of ammonium sulfate and 5kg/T of sodium chloride;
the fermentation conditions of the culture medium are as follows: at 37 ℃, the pH value is 7.0, the tank pressure is 1Kg, the ventilation volume is 80 cubic/h, the stirring is 80rpm, the dissolved oxygen is 30 percent, and the fermentation time is about 24 hours;
the preparation method of the bacillus cereus comprises the following steps:
the culture medium is as follows:
10kg/T of cane sugar, 8kg/T of bean flour, 5kg/T of peptone, 1kg/T of beef extract, 3kg/T of sodium chloride, 1kg/T of ammonium sulfate and 2kg/T of monopotassium phosphate;
the fermentation conditions of the culture medium are as follows: at 37 ℃, the pH value is 7.0, the tank pressure is 1Kg, the ventilation volume is 80 cubic/h, the stirring is 80rpm, the dissolved oxygen is 30 percent, and the fermentation time is about 24 hours;
the preparation method of the bacillus amyloliquefaciens comprises the following steps:
the culture medium is as follows:
10Kg/T of cane sugar, 6g/T of bean flour, 5Kg/T of peptone, 1Kg/T of beef extract, 5Kg/T of sodium chloride, pH7.0 and 2Kg/T of antifoaming agent;
the fermentation conditions of the culture medium are as follows: inoculating the shake flask seeds into a fermentation tank according to the inoculation amount of 1%, wherein the volume of the fermentation tank is 5 tons, the fermentation parameters of the fermentation tank are 37 ℃, the pH value is 7.0, the tank pressure is 1Kg, the ventilation volume is 80 cubic/hour, the stirring is 80rpm, and the dissolved oxygen fermentation time is about 24 hours by 30%;
the preparation method of the krusei candida and the crinkliniella candida comprises the following steps:
the culture medium is as follows:
10kg/T peptone, 8kg/T sucrose, 2kg/T glucose, KH2PO4 1kg/T，MgSO4·7H2O 0.5kg/T，NaCl 5kg/T；
The fermentation conditions of the culture medium are as follows: 37 ℃, pH 7.0-7.2, tank pressure 0.05MPa, ventilation volume 10-15 cubic/h and fermentation time about 24 hours.
5. The microbial agent for treating aquaculture sewage according to claim 1, which is characterized in that:
the preparation method of the bacillus methanolicus comprises the following steps:
the formula of the culture medium is as follows:
peptone 1%, yeast powder 0.5%, corn flour 0.5%, sodium chloride 0.1%, potassium dihydrogen phosphate 0.05%, ammonium chloride 0.025%, separately-dissolved calcium chloride 0.05%, defoamer 0.05%, natural pH;
the fermentation process comprises the following steps: the culture temperature is as follows: 35 ℃, initial rotation speed: 80r/min, the ventilation volume is about 100L/h, the stirring can be stopped after 6-8 hours, the ventilation volume is increased to about 110L/h, and the fermentation period is 16-20 hours;
the preparation method of the rhodopseudomonas palustris comprises the following steps:
the formula of the culture medium is as follows:
the formula of the culture medium is as follows: CH (CH)3COONa 3g/L，NaHCO3 5g/L，(NH4)2SO41g/L, peptone 8g/L, yeast extract 1g/L, MgSO40.5g/L，NaCl 2g/L，KH2PO4 1g/L，CaCl2 0.5g/L，pH 7.2；
The fermentation process comprises the following steps: temperature: 30 ℃, initial rotation speed: 100r/min, ventilation capacity of about 100L/H, stirring at 150r/min after 6-8 hours, keeping the ventilation capacity unchanged, and putting into a tank after 18-20 hours.
6. The microbial agent for treating aquaculture sewage according to claim 1, which is characterized in that:
the preparation method of the pseudomonas syringae comprises the following steps:
the formula of the culture medium is as follows:
10g/L of tryptone, 10ml/L of glucose, 0.5% of corn flour, 1g/L of sodium chloride, 0.8g/L of monopotassium phosphate, 1.5g/L of magnesium sulfate, 1g/L of separately dissolved calcium chloride and natural pH;
the fermentation process comprises the following steps: the culture temperature is as follows: 30 ℃, initial rotation speed: 100r/min, the ventilation capacity is about 120L/h, stirring is carried out at 150r/min after 6-8 hours, the ventilation capacity is unchanged, and the fermentation period is 32 hours;
the preparation method of the stenotrophomonas comprises the following steps:
the formula of the culture medium is as follows:
the formula of the culture medium is as follows: 1% of soybean meal, 1% of corn flour, 0.3% of yeast powder, 0.2% of sodium chloride, 0.05% of monopotassium phosphate, 0.025% of ammonium sulfate, 0.05% of separately dissolved calcium chloride, 0.05% of defoaming agent, proper amount of protease and amylase and natural pH;
the fermentation process comprises the following steps: the culture temperature is as follows: 35 ℃, initial rotation speed: the aeration rate is 100L/H at 80r/min, the stirring can be stopped after 6-8 hours, the aeration rate is increased to about 110L/H, and the fermentation period is 16-20 hours.
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Denomination of invention: A microbial agent for treating aquaculture wastewater Granted publication date: 20211126 Pledgee: Agricultural Bank of China Limited Shanghai Songjiang Sub-branch Pledgor: SHANGHAI GUOLONG BIOTECHNOLOGY CO.,LTD. Registration number: Y2024310000212 |