CN114015611A - Microbial composition and method for treating wastewater by using same - Google Patents
Microbial composition and method for treating wastewater by using same Download PDFInfo
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/20—Bacteria; Culture media therefor
-
- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F3/00—Biological treatment of water, waste water, or sewage
- C02F3/34—Biological treatment of water, waste water, or sewage characterised by the microorganisms used
-
- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05F—ORGANIC FERTILISERS NOT COVERED BY SUBCLASSES C05B, C05C, e.g. FERTILISERS FROM WASTE OR REFUSE
- C05F11/00—Other organic fertilisers
-
- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05F—ORGANIC FERTILISERS NOT COVERED BY SUBCLASSES C05B, C05C, e.g. FERTILISERS FROM WASTE OR REFUSE
- C05F17/00—Preparation of fertilisers characterised by biological or biochemical treatment steps, e.g. composting or fermentation
- C05F17/20—Preparation of fertilisers characterised by biological or biochemical treatment steps, e.g. composting or fermentation using specific microorganisms or substances, e.g. enzymes, for activating or stimulating the treatment
-
- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F2103/00—Nature of the water, waste water, sewage or sludge to be treated
- C02F2103/34—Nature of the water, waste water, sewage or sludge to be treated from industrial activities not provided for in groups C02F2103/12 - C02F2103/32
- C02F2103/36—Nature of the water, waste water, sewage or sludge to be treated from industrial activities not provided for in groups C02F2103/12 - C02F2103/32 from the manufacture of organic compounds
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02W—CLIMATE CHANGE MITIGATION TECHNOLOGIES RELATED TO WASTEWATER TREATMENT OR WASTE MANAGEMENT
- Y02W30/00—Technologies for solid waste management
- Y02W30/40—Bio-organic fraction processing; Production of fertilisers from the organic fraction of waste or refuse
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- Biotechnology (AREA)
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- Water Supply & Treatment (AREA)
- Chemical Kinetics & Catalysis (AREA)
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Abstract
The invention discloses a microbial composition and a method for treating wastewater by using the same. A microbial composition for treating wastewater, the microbial composition comprising at least two of pseudomonas stutzeri, streptomyces erythreus, streptomyces albus, bacillus subtilis, bacillus mucilaginosus, and actinomyces halioticus; the wastewater satisfies one or both of the following conditions: COD value reaches 90000-110000 mg/L; b. the total nitrogen value reaches 3000-5000 mg/L. The method for culturing the biological composition by using the taurine industrial wastewater provided by the invention has low cost and good effect; the waste liquid in the taurine production is used for culturing the microbial composition, so that COD (COD which can be degraded by more than 95 percent at most) and total nitrogen in the waste water can be reduced; the waste water can be used for culturing the microbial composition, and the bacteria obtained by fermentation can be used as a biological fertilizer, so that the fertilizer has a fertility-increasing effect on vegetables and green plants.
Description
Technical Field
The invention belongs to the field of chemical fertilizer manufacturing and wastewater treatment, relates to a microbial composition and a method for treating wastewater by using the same, and particularly relates to a method for culturing probiotics for a biological fertilizer by using taurine industrial wastewater.
Background
Taurine is widely used in the fields of medicines, food additives, feeds, surfactants, whiteners, and the like, and its application range is expanding, and in recent years, supply and demand are on the spot. But the production wastewater contains high-concentration COD and NH4+N, organic nitrogen, and salts such as ammonium sulfate or ammonium chloride. If the sewage is directly delivered to a sewage station without treatment, the sewage can be treated only after the pollution factors are diluted in a large amount until the pollution factors meet the water inlet standard, so that the water treatment amount is multiplied, and very high treatment cost is brought.
In China, a lot of research works are done on the treatment of wastewater from taurine production, such as electrodialysis, liquid membrane method, wastewater reclamation and other treatment technologies. However, the treatment cost of the technologies is high, the impact load resistance is poor, and the effluent quality can not meet the requirements, so that the technologies are limited in engineering application.
In order to solve the problem of wastewater generated in the production process of taurine, patent CN109053326A discloses a method for preparing a granular nitrogen fertilizer by using nitrogen-containing wastewater in the industrial production of taurine.
Patent CN102649655B discloses a charcoal-based compound fertilizer containing probiotics, which discloses that the prepared fermentation liquor is utilized firstly to enlarge and culture the probiotics, then the probiotics are filtered out, the probiotics are sprayed and added into biomass carbon black, a granular charcoal-based compound fertilizer is obtained after treatment, and the purpose of fertilizer synergism can be achieved after the probiotics are added. But the cost is higher because the probiotics are subjected to fermentation liquor expansion culture.
In patent CN110483342A, a method for removing impurities and recovering taurine mother liquor is disclosed, in which the taurine mother liquor is subjected to activated carbon impurity removal in the first step, resin impurity removal in the second step, ammonia desalination in the third step, and the filtered mother liquor is recovered after filtration. The method disclosed in the patent is very complicated and cannot completely remove impurities, and after the impurities are circularly accumulated in the system, the wastewater with high COD and ammonia nitrogen still needs to be discharged and treated.
As an active component of soil, the regional composition, biomass and life activities of soil microorganisms have a close relationship with the formation and development of soil. For example, some soil microorganisms may form soil structures, some may decompose minerals, some may also fix nitrogen in the air, and the like. The soil manager can purposefully regulate the number and the types of soil microorganisms through various measures according to actual conditions and make agricultural production measures; and a farming system can be made according to the objective conditions of soil microorganisms. The original microbial community is restored or certain functions are added by measures of improving fertilization and cultivation systems, artificially introducing beneficial soil microorganisms and the like, so that the soil-borne disease bacteria of crops are inhibited, the diversity of the soil microorganisms is improved, the improvement of the ecological fertility of the soil is facilitated, and the soil-borne disease and the continuous cropping obstacle of the crops are fundamentally prevented and treated.
In order to achieve the effect of resource utilization, a method for treating taurine industrial wastewater with low cost and simple operation is urgently needed at present.
Disclosure of Invention
In order to solve the above technical problems, the present invention provides a microbial composition and a method for treating wastewater using the same. The method for culturing the biological composition by using the taurine industrial wastewater provided by the invention has the advantages of low cost and good effect; the waste liquid in the taurine production is used for culturing the microbial composition, so that COD (COD which can be degraded by more than 95 percent at most) and total nitrogen in the waste water can be reduced; and the waste water can be used for culturing a microorganism composition (the composition can survive in an environment with a COD value of more than 100000 mg/L), and bacteria obtained by fermentation can be used as a biological fertilizer and has a fattening effect on vegetables and green plants.
The present invention provides in a first aspect a microbial composition for treating wastewater comprising at least two of Pseudomonas stutzeri, Streptomyces rubra (Streptomyces ruber), Streptomyces albus (Streptomyces albus), Bacillus subtilis, Bacillus mucilaginosus (Streptomyces griseus var. ferugineus), and actinomyces halioticus (Haloactinomyces sp.), the wastewater satisfying one or both of the following conditions:
COD value reaches 90000-110000 mg/L;
b. the total nitrogen value reaches 3000-5000 mg/L.
Wherein, the COD value is preferably 100000 mg/L.
Wherein, the total nitrogen value is preferably 4000 mg/L.
The pseudomonas stutzeri is preferably pseudomonas stutzeri with the number of bio-17137 on the Chinese microbial strain inquiry network platform.
The streptomyces erythreus is preferably the streptomyces erythreus numbered bio-096964 on the Chinese microbial strain inquiry network platform.
The streptomyces albus is preferably selected from the streptomyces albus with the number of bio-18862 on the Chinese microbial strain inquiry network platform.
The bacillus subtilis is preferably bacillus subtilis with the number of bio-62321 on a platform of a Chinese microbial strain inquiry network.
The bacillus mucilaginosus is preferably bacillus mucilaginosus with the number of bio-62177 on the platform of the Chinese microbial strain inquiry network.
The halophilic actinomycetes is preferably the halophilic actinomycetes with the product number of BNCC218473 in the research center of labor-saving microbial strain engineering technology in Henan.
In a preferred embodiment, the microbial composition comprises Streptomyces rubrus and Bacillus mucilaginosus, or comprises Streptomyces rubrus and Actinomyces halophilus, or comprises Streptomyces albus and Pseudomonas stutzeri, or comprises Streptomyces rubrus and Bacillus subtilis.
In a second aspect, the present invention provides a method of treating wastewater, the method comprising mixing the wastewater with the microbial composition of the first aspect of the invention, and culturing the microbial composition, the wastewater satisfying one or both of the following conditions:
COD value reaches 90000-110000 mg/L;
b. the total nitrogen value reaches 3000-5000 mg/L.
Wherein, the COD value is preferably 100000 mg/L.
Wherein, the total nitrogen value is preferably 4000 mg/L.
In a preferred embodiment, the wastewater is wastewater from the desalination of ammonia in the production of taurine.
The method preferably comprises the steps of:
(1) taking the wastewater, and diluting by 1-20 times; preferably 1 to 5 times;
(2) inoculating 1-10% of microorganism for fermentation; preferably 2% to 8%, more preferably 4%, the percentages being by volume.
The dilution may be conventional in the art, such as dilution with water.
In a preferred embodiment, step (1) comprises a step of removing ammonia by evaporation.
In the method, the step (2) preferably includes a step of supplementing glucose during the fermentation.
In the method, the pH of the fermentation is preferably 7 to 9, more preferably 7.5.
In the method, the temperature of the fermentation is preferably 25 to 30 ℃.
In the method, the fermentation time is preferably 6-36h, more preferably 24 h.
In a third aspect, the present invention provides a method for microbial fermentation, the method comprising mixing wastewater with the microbial composition according to the first aspect of the present invention, and culturing the microbial composition, the wastewater satisfying one or both of the following conditions:
COD value reaches 90000-110000 mg/L;
b. the total nitrogen value reaches 3000-5000 mg/L.
Wherein, the COD value is preferably 100000 mg/L.
Wherein, the total nitrogen value is preferably 4000 mg/L.
In a preferred embodiment, the wastewater is wastewater from the desalination of ammonia in the production of taurine.
The method preferably comprises the steps of:
(1) taking the wastewater, and diluting by 1-20 times; preferably 1 to 5 times;
(2) inoculating 1-10% of microorganism for fermentation; preferably 2% to 8%, more preferably 4%, the percentages being by volume.
The dilution may be conventional in the art, such as dilution with water.
In a preferred embodiment, step (1) comprises a step of removing ammonia by evaporation.
In the method, the step (2) preferably includes a step of supplementing glucose during the fermentation.
In the method, the pH of the fermentation is preferably 7 to 9, more preferably 7.5.
In the method, the temperature of the fermentation is preferably 25 to 30 ℃.
In the method, the fermentation time is preferably 6-36h, more preferably 24 h.
The fourth aspect of the invention provides a preparation method of a biological fertilizer, which comprises the following steps:
(1) performing fermentation using a method according to the third aspect of the invention;
(2) removing the fermentation liquor after the fermentation is finished to obtain thalli;
the method for preparing the biological waste preferably further comprises composting the bacteria.
The composting is preferably carried out in wheat straw.
In a fifth aspect, the present invention provides a biofertilizer obtained by the production method according to the fourth aspect of the present invention.
In a sixth aspect, the present invention provides the use of a biological fertilizer according to the fifth aspect of the invention in plant cultivation.
In such applications, the plant is preferably a vegetable or green plant, such as a wax gourd, a pumpkin or tomato, or a scindapsus aureus.
On the basis of the common knowledge in the field, the above preferred conditions can be combined randomly to obtain the preferred embodiments of the invention.
The reagents and starting materials used in the present invention are commercially available.
The technical principle and mechanism of the invention are explained as follows:
1. the taurine production waste liquid contains high COD substances and ammonia nitrogen substances, COD can be used as a carbon source of probiotics, and ammonia nitrogen can be used as a nitrogen source of the probiotics;
2. as an active component of soil, the regional composition, biomass and life activities of soil microorganisms have a close relationship with the formation and development of soil. For example, some soil microorganisms may form soil structures, some may decompose minerals, some may also fix nitrogen in the air, and the like.
The pseudomonas stutzeri, the streptomyces erythreus, the streptomyces albus, the bacillus subtilis, the bacillus mucilaginosus and the actinomyces halophilus can be obtained from the China agricultural microbial strain preservation center, the China strain preservation center ATCC, the China industrial microbial strain preservation center CICC, the China general microbial strain preservation management center CGMCC, and the Henan labor-saving microbial strain engineering research center, and can also be extracted from soil near a sewage station by using a conventional method.
For example, the extraction method of bacillus subtilis comprises the following steps: (1) taking a soil sample at a drainage port of a taurine sewage station (or a wastewater discharge port of other amino acids), and uniformly dispersing the soil sample in sterile water; (2) taking the supernatant in the suspension in the step (1), diluting, coating the supernatant on a flat plate of a basic culture medium prepared from sterile soil extract, and carrying out inverted culture in an incubator at 37 ℃ for 24-48 hours and 4 separation; (3) screening corresponding bacillus subtilis by using a selective culture medium, or heating in water bath to 80 ℃ after culture for 20min, then coating, wherein the bacillus subtilis can survive due to heat resistance after forming spores, and selecting a single colony for microscopic examination after coating a flat plate; (4) inoculating the screened bacillus subtilis to a liquid culture medium suitable for the growth of the bacillus subtilis, and propagating the bacillus subtilis in a large quantity.
The method for screening the streptomyces erythreus comprises the following steps: (1) taking a soil sample in a place suitable for the growth of the streptomyces erythreus, preferably at a drainage port of a taurine sewage station (or a wastewater discharge port of other amino acids), and uniformly dispersing the soil sample in sterile water; (2) taking supernatant in the suspension, diluting, coating on a flat plate of a basic culture medium prepared from sterile soil extract, and carrying out inverted culture in an incubator at 37 ℃ for 24-48 hours; (3) screening corresponding streptomyces erythreus with selective medium (ISP2 medium, TSBY medium); (4) inoculating the screened streptomyces erythreus to a liquid culture medium suitable for the growth of the streptomyces erythreus, and enabling the streptomyces erythreus to propagate in a large amount.
The positive progress effects of the invention are as follows:
the method for culturing the biological composition by using the taurine or amino acid industrial wastewater provided by the invention has the advantages of low cost and good effect; the waste liquid in taurine production (or the waste liquid in amino acid production) is used for culturing the microbial composition, so that COD (chemical oxygen demand (COD) value of the composition can be survived under the environment of more than 100000mg/L, and the highest COD can be degraded by more than 95 percent) and total nitrogen in the waste water can be reduced; the waste water can be used for culturing the microbial composition, and the bacteria obtained by fermentation can be used as a biological fertilizer, so that the fertilizer has a fertility-increasing effect on vegetables and green plants.
Drawings
FIG. 1 is a process flow diagram of the present invention.
Detailed Description
The invention is further illustrated by the following examples, which are not intended to limit the scope of the invention. The experimental methods without specifying specific conditions in the following examples were selected according to the conventional methods and conditions, or according to the commercial instructions.
Example 1:
the process flow diagram of the present invention refers to figure 1. Taking taurine mother liquor with COD value of 100000mg/L after ammonia is desalted and total nitrogen of 4000mg/L, diluting by 1 time, if the ammonia smell of the solution is larger, removing partial ammonia by evaporation until no ammonia smell overflows, cooling, adding purified water to the original scale before ammonia removal, adjusting pH to about 7.5 by hydrochloric acid, inoculating 2% of streptomyces erythreus (bio-096964) and 2% of bacillus mucilaginosus (bio-62117), controlling fermentation conditions, supplementing glucose in proper amount when the strain grows slowly, maintaining the fermentation temperature at 25-30 ℃, sampling and measuring OD value or wet weight in the fermentation process, centrifuging, taking supernatant and measuring COD value and total nitrogen, and obtaining the results as the following table 1:
TABLE 1
| Time | Glucose | COD value | Total nitrogen number |
| 0h | 0g/L | 55000 | 1950 |
| 2h | 0g/L | 46000 | 1700 |
| 4h | 0g/L | 43000 | 1500 |
| 6h | 2g/L | 21500 | 1100 |
| 12h | 0g/L | 7230 | 570 |
| 24h | 0g/L | 2400 | 350 |
From example 1, it can be seen that 95.6% of COD and 82% of total nitrogen in the waste stream can be consumed after 24 hours of fermentation by diluting the waste stream once after ammonia desalination, inoculating 2% of Streptomyces rubiginis (bio-096964) and 2% of Bacillus mucilaginosus (bio-62117).
Example 2:
taking taurine mother liquor with COD value of 100000mg/L after ammonia desalination and total nitrogen of 4000mg/L, diluting 1 time, removing partial ammonia by evaporation if the ammonia taste of the solution is larger, cooling, adding purified water to the original scale before ammonia removal, adjusting pH to about 7.5 by hydrochloric acid, inoculating 2% of streptomyces erythreus (bio-096964) and 2% of actinomyces halophilus (BNCC218473), controlling fermentation conditions, supplementing glucose in proper amount when the strain grows slowly, maintaining fermentation temperature at 25-30 ℃, sampling and measuring OD value or wet weight during fermentation, centrifuging and taking supernatant to measure COD value and total nitrogen, and the results are as the following table 2:
TABLE 2
| Time | Glucose | COD value | Total nitrogen number |
| 0h | 0g/L | 55000 | 1950 |
| 2h | 0g/L | 42000 | 1600 |
| 4h | 0g/L | 31000 | 1300 |
| 6h | 0g/L | 19500 | 990 |
| 12h | 0g/L | 4300 | 460 |
| 24h | 0g/L | 1600 | 240 |
By example 2, it can be found that 97.1% of COD and 87.7% of total nitrogen in the waste liquid can be consumed after the waste liquid after ammonia desalination is diluted by one time, inoculated with 2% of streptomyces erythreus and 2% of actinomyces halophilus and fermented for 24 h.
Example 3:
taking taurine mother liquor with COD value of 100000mg/L after ammonia desalination and total nitrogen of 4000mg/L, diluting 1 time, if the ammonia smell of the solution is larger, removing partial ammonia by evaporation, cooling, adding purified water to the original scale before ammonia removal, adjusting pH to about 7.5 by hydrochloric acid, inoculating 4% of streptomyces albus (bio-18862) and 4% of pseudomonas stutzeri strain (bio-17137), controlling fermentation conditions, supplementing glucose in a proper amount when the strain is slowly increased, maintaining the fermentation temperature at 25-30 ℃, sampling and measuring OD value/wet weight in the fermentation process, centrifuging and taking supernatant to measure COD value and total nitrogen, and the results are shown in the following table 3:
TABLE 3
From example 3 it can be seen that the effluent from the ammonia desalination was diluted one time, inoculated with 4% of Streptomyces albus (bio-18862) and 4% of Pseudomonas stutzeri strain (bio-17137), and after 36h of fermentation 67.6% of the COD in the effluent and 68.7% of the total nitrogen were consumed.
Example 4:
taking taurine mother liquor with COD value of 100000mg/L and total nitrogen of 4000mg/L after ammonia is desalted, diluting by 5 times, adjusting pH to 7.5 by hydrochloric acid, inoculating 4% of streptomyces erythreus (bio-096964) and 4% of bacillus subtilis (bio-62321), controlling fermentation conditions, supplementing proper amount of glucose when the strain grows slowly, maintaining the fermentation temperature at 25-30 ℃, sampling and measuring OD value/wet weight in the fermentation process, centrifuging, taking supernatant to measure COD value and total nitrogen, and obtaining the results as shown in the following table 4:
TABLE 4
| Time | Glucose | COD value | Total nitrogen number |
| 0h | 0g/L | 23000 | 860 |
| 2h | 0g/L | 22000 | 820 |
| 4h | 2g/L | 15700 | 740 |
| 6h | 2g/L | 10900 | 660 |
| 12h | 2g/L | 6800 | 410 |
| 24h | 0g/L | 3900 | 310 |
| 36h | 0g/L | 2400 | 220 |
Through example 4, it can be found that, after the waste liquid after ammonia desalination is diluted by 5 times, inoculated with streptomyces erythreus (bio-096964) and 4% bacillus subtilis (bio-62321), fermented for 24 hours, 83% of COD and 64.4% of total nitrogen in the waste liquid can be consumed, and the fermentation is continued to 36 hours, 89.5% of COD and 74.4% of total nitrogen in the waste liquid can be consumed, so that the fermentation time is prolonged, and the effects of removing COD and nitrogen are not obviously increased.
Example 5:
taking taurine mother liquor with COD value of 100000mg/L and total nitrogen of 4000mg/L after ammonia desalination, diluting 20 times, adjusting pH to 7.5 by hydrochloric acid, inoculating 8% of streptomyces erythreus (bio-096964) and 8% of actinomyces halioticus (BNCC218473), controlling fermentation conditions, supplementing proper amount of glucose when the strain grows slowly, maintaining the fermentation temperature at 25-30 ℃, taking samples during the fermentation process, measuring OD value/wet weight, centrifuging, taking supernatant to measure COD value and total nitrogen, and obtaining the results as shown in the following table 5:
TABLE 5
| Time | Glucose | COD value | Total nitrogen number |
| 0h | 0g/L | 5200 | 230 |
| 2h | 0g/L | 4700 | 190 |
| 4h | 0g/L | 4300 | 120 |
| 6h | 0g/L | 2200 | 60 |
As can be seen from example 5, even if the fermentation broth is diluted 20 times, the optimum combination of microorganisms (Streptomyces rubripes and Actinomyces halophilus) selected by the present invention can degrade COD and total nitrogen under the condition of very low nutrient content.
Example 6:
taking taurine mother liquor with COD value of 100000mg/L after ammonia desalination and total nitrogen of 4000mg/L, diluting 1 time, if the ammonia smell of the solution is larger, removing partial ammonia by evaporation, cooling, adding purified water to the original scale before ammonia removal, adjusting pH to about 7.5 by hydrochloric acid, inoculating 4% Bacillus mucilaginosus (bio-62117), controlling fermentation conditions, supplementing proper amount of glucose when the strain grows slowly, maintaining the fermentation temperature at 25-30 ℃, taking samples to measure OD value or wet weight in the fermentation process, taking supernatant to measure COD value and total nitrogen after centrifugation, and obtaining the following results as table 6:
TABLE 6
| Time | Glucose | COD value | Total nitrogen number |
| 0h | 0g/L | 55000 | 1950 |
| 2h | 0g/L | 54000 | 1860 |
| 4h | 2g/L | 53000 | 1780 |
| 6h | 2g/L | 49500 | 1690 |
| 12h | 2g/L | 47600 | 1540 |
| 24h | 0g/L | 41700 | 1360 |
Example 7:
taking taurine mother liquor with COD value of 100000mg/L after ammonia is desalted and total nitrogen of 4000mg/L, diluting by 1 time, if the ammonia smell of the solution is larger, removing partial ammonia by evaporation, cooling, adding purified water to the scale before the original ammonia is removed, adjusting pH to 7.5 by hydrochloric acid, inoculating 4% streptomyces erythreus (bio-096964), controlling fermentation condition, supplementing proper amount of glucose when the strain is slowly increased, maintaining the fermentation temperature at 25-30 ℃, taking samples to measure OD value or wet weight in the fermentation process, centrifuging, taking supernatant to measure COD value and total nitrogen, and obtaining the following results as table 7:
TABLE 7
| Time | Glucose | COD value | Total nitrogen number |
| 0h | 0g/L | 55000 | 1950 |
| 2h | 0g/L | 52000 | 1770 |
| 4h | 2g/L | 51000 | 1630 |
| 6h | 2g/L | 44500 | 1520 |
| 12h | 2g/L | 38400 | 1210 |
| 24h | 0g/L | 41700 | 1360 |
Example 8:
taking taurine mother liquor with COD value of 100000mg/L after ammonia desalination and total nitrogen of 4000mg/L, diluting 1 time, if the ammonia taste of the solution is larger, removing partial ammonia by evaporation, cooling, adding purified water to the original scale before ammonia removal, adjusting pH to about 7.5 by hydrochloric acid, inoculating 2% streptomyces erythreus (bio-096964), 2% bacillus mucilaginosus (bio-62117) and 2% actinomyces halophilus (BNCC218473) to control fermentation conditions, supplementing glucose in right amount when the strain grows slowly, maintaining the fermentation temperature at 25-30 ℃, sampling and measuring OD value or wet weight in the fermentation process, centrifuging, taking supernatant liquid and measuring COD value and total nitrogen, and obtaining the following results as the following table 8:
TABLE 8
| Time | Glucose | COD value | Total nitrogen number |
| 0h | 0g/L | 55000 | 1950 |
| 2h | 0g/L | 49000 | 1810 |
| 4h | 0g/L | 44000 | 1720 |
| 6h | 2g/L | 37200 | 1570 |
| 12h | 2g/L | 39100 | 1410 |
| 24h | 0g/L | 41600 | 1340 |
As can be seen from examples 6-8, even if glucose is continuously supplemented to the fermentation broth when only 1 kind of bacteria is inoculated, the decomposition of COD and nitrogen-containing substances by the bacteria cannot be promoted well, and in addition, 32.4% of COD can be degraded in the first 6 hours by 2% of Streptomyces rubiginus (bio-096964), 2% of Bacillus mucilaginosus (bio-62177) and 2% of actinomyces halophilus (BNCC218473) in the fermentation broth, but the COD is increased slowly after 12 hours, and it is presumed that some kinds of bacteria may have cell wall breakage during the competitive growth of the bacteria.
Example 9
Taking dry wheat straws, adding a mixture of streptomyces erythreus and bacillus mucilaginosus cultured in example 1 in a weight ratio of 1%, spraying water in a weight ratio of 1%, uniformly stirring, aerating for 24 hours, and composting for 30 days under an aerobic condition. Spreading and cooling to obtain the fertilizer for green plants.
Example 10
Taking 4 pots of soil-cultured scindapsus aureus with similar growth vigor cultured under the same greenhouse condition, and uniformly spraying 50g of 0.1% urea solution on the soil surface of the 4 pots of scindapsus aureus. The 4 pots of scindapsus aureus are evenly divided into a group A and a group B, 50g of the fertility intensifier prepared in the example 9 is evenly paved on the soil of the two pots of scindapsus aureus in the group A, 50g of wheat straw ash is evenly paved on the soil of the two pots of scindapsus aureus in the group B, and the two pots of scindapsus aureus in the group B are cultured under the same room temperature condition, so that yellow leaves appear only after 30 days of culture, but yellow leaves appear after 15 days of culture of the two pots of scindapsus aureus in the group B.
In a laboratory, the scindapsus aureus is replaced by wax gourd, pumpkin and tomato, and the same comparison experiment is carried out, so that the fertilizer prepared by the invention has the effect of increasing the weight.
Claims (10)
1. A microbial composition for treating wastewater comprising at least two of Pseudomonas stutzeri, Streptomyces rubra (Streptomyces ruber), Streptomyces albus (Streptomyces albus), Bacillus subtilis, Bacillus mucilaginosus (Streptomyces griseus var. ferugineus), and actinomyces halioticus (Haloactinomyces sp.), the wastewater satisfying one or both of the following conditions:
COD value of 90000-110000mg/L, preferably 100000 mg/L;
b. the total nitrogen value reaches 3000-5000 mg/L; preferably 4000 mg/L;
preferably, the microbial composition comprises streptomyces erythreus and bacillus mucilaginosus, or comprises streptomyces erythreus and actinomyces halophilus, or comprises streptomyces albus and pseudomonas stutzeri, or comprises streptomyces erythreus and bacillus subtilis.
2. The microbial combination of claim 1, wherein the pseudomonas stutzeri has platform number bio-17137;
and/or the platform number of the streptomyces erythreus in the Chinese microbial strain query network is bio-096964;
and/or the streptomyces albus is numbered bio-18862 on the platform of the Chinese microbial strain inquiry network;
and/or the bacillus subtilis is numbered as bio-62321 on the platform of the Chinese microbial strain inquiry network;
and/or the Bacillus mucilaginosus is numbered bio-62177 on the platform of the Chinese microbial strain inquiry network;
and/or the product number of the salt-loving actinomycete in the research center of labor-saving microbial strain engineering technology in Henan is BNCC 218473.
3. A method of treating wastewater, comprising mixing the wastewater with the microbial composition of claim 1 or 2, and culturing the microbial composition, wherein the wastewater satisfies one or both of the following conditions:
COD value of 90000-110000mg/L, preferably 100000 mg/L;
b. the total nitrogen value reaches 3000-5000 mg/L; preferably 4000 mg/L.
4. The method of claim 3, wherein the wastewater is wastewater from the desalination of ammonia in the production of taurine; and/or, the method comprises the steps of:
(1) taking the wastewater, and diluting by 1-20 times; preferably 1 to 5 times;
(2) inoculating 1-10% of the microbial composition for fermentation; preferably 2% to 8%, more preferably 4%, wherein the percentages are volume percentages.
5. The method of claim 4, wherein the method satisfies one or more of the following conditions:
the step (1) comprises a step of removing ammonia by using an evaporation method;
the step (2) comprises the step of supplementing glucose in the fermentation process;
the pH of the fermentation is 7-9, preferably 7.5;
the fermentation temperature is 25-30 ℃;
the fermentation time is 6-36h, preferably 24 h.
6. A method of microbial fermentation, comprising mixing wastewater with the microbial composition of claim 1 or 2, and culturing the microbial composition, wherein the wastewater satisfies one or both of the following conditions:
COD value of 90000-110000mg/L, preferably 100000 mg/L;
b. the total nitrogen value reaches 3000-5000 mg/L; preferably 4000 mg/L.
7. The method of claim 6, wherein the wastewater is wastewater from the desalination of ammonia in the production of taurine; and/or, the method comprises the steps of:
(1) taking the waste water, and diluting by 1-20 times; preferably 1 to 5 times;
(2) inoculating 1-10% of the microbial composition for fermentation; preferably 2% to 8%, more preferably 4%, wherein the percentages are volume percentages.
8. The method of claim 7, wherein the method satisfies one or more of the following conditions:
the step (1) comprises a step of removing ammonia by using an evaporation method;
the step (2) comprises the step of supplementing glucose in the fermentation process;
the pH of the fermentation is 7-9, preferably 7.5;
the fermentation temperature is 25-30 ℃;
the fermentation time is 6-36h, preferably 24 h.
9. A preparation method of a biological fertilizer comprises the following steps:
(1) fermenting using the method of any one of claims 6-8;
(2) removing the fermentation liquor after the fermentation is finished to obtain thalli;
preferably, the preparation method further comprises composting the thalli; more preferably, the composting is carried out in wheat straw.
10. A biofertilizer obtained by the production method according to claim 9; preferably, the plant is a vegetable or green plant;
wherein, the vegetable is preferably wax gourd, pumpkin or tomato, and the green plant is preferably scindapsus aureus.
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