CN101269884A - Microorganism renovation agent of water environment and preparation method thereof - Google Patents
Microorganism renovation agent of water environment and preparation method thereof Download PDFInfo
- Publication number
- CN101269884A CN101269884A CNA2008100251358A CN200810025135A CN101269884A CN 101269884 A CN101269884 A CN 101269884A CN A2008100251358 A CNA2008100251358 A CN A2008100251358A CN 200810025135 A CN200810025135 A CN 200810025135A CN 101269884 A CN101269884 A CN 101269884A
- Authority
- CN
- China
- Prior art keywords
- renovation agent
- microorganism
- less
- fermentor tank
- bacterium
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
Abstract
The invention belongs to the applied biology technical field, and relates to a water environmental microbiology reparation agent and a preparation method thereof. The reparation agent has the mass percent of the components in the microbiology total weight as follows: bacillus subtilis is 30-40 percent, bacillus licheniformis is 30-40 percent, ray fungi is 10-20 percent, denitrifying bacterium is 10-20 percent, and photosynthetic bacterium is 10-20 percent. The microbiology preparation of the invention has strong adaptability, can compensate the water body original bacterium when being put in water, quickly form the preponderant flora in the water body, produce the high-activity biological enzymes for quickening the decomposition of the left diet and the animal wastes, and produce the antibiotic substance to restrain the growing of harmful bacterium and reduce the occurrence of the illness as well as ensure the production of green marine products.
Description
Technical field
The invention belongs to the Applied Biotechnology field, relate to a kind of microorganism renovation agent of water environment and preparation method thereof.
Background technology
Appearance along with the aquatic products intensive culture, cultivation density significantly improves, cause that residual bait and animal excrements increase in the water body, under such envrionment conditions, impel microorganism to breed (comprising pathogenic bacterium), COD, ammonia-nitrogen content rising, dissolved oxygen decline in a large number, breeding environment constantly worsens, and makes culture fishery take a bath.Water quality factor is more and more noticeable to the influence of culturing success or failure and output.Water quality is good, and pathogenic bacteria is few, and the hydrocoles growth nature of breed is all right; And condition of water quality is poor, the toxic substance accumulation, and pathogenic bacteria is many and virulence is strong, and the hydrocoles of breed is just encroached on easily; Simultaneously, owing to exist the effect of giving birth to of competing between the similar biology, when in aquaculture water, drop into selectively a certain amount of favourable water quality improvement, can water of decomposition in organism and objectionable impurities and to aquaculture organism behind the harmless microbial preparation or bacterial classification, a kind of new equilibrium relationship is set up, water quality is improved, growth of pathogenic bacteria is suppressed, and aquaculture organism just can grow healthily.
Microbial preparation existing institute on aquatic products used in recent years, but existed a lot of problems, was water surrounding microorganisms in addition as some, and these microorganisms have arrived can not adapt to water body environment in the water body, can not become the dominant microflora of water body rapidly; In addition, some microorganisms can not produce enzyme, are the small molecules nutritive substance of simple absorption water body, can not degrade effectively residual bait and ight soil, and effect is slow.
Summary of the invention:
The objective of the invention is problem, a kind of microorganism renovation agent of water environment that contains biological enzyme is provided at the existence in the aquatic environment improvement of above-mentioned probiotics.
Another object of the present invention provides the preparation method of above-mentioned microorganism renovation agent of water environment.
The objective of the invention is to realize by following technical measures:
A kind of microorganism renovation agent of water environment, the mass percent of each component microorganism of this renovation agent in the microbial total quality is: subtilis 30%～40%, Bacillus licheniformis 30%～40%, actinomycetes 10～20%, denitrifying bacterium 10～20%, photosynthetic bacterium 10～20%.Each microorganism component sum is 100% described in the specific product.
Described microorganism renovation agent of water environment, bacterial content is not less than 5.0 * 10 in every gram or this microorganism renovation agent of every ml
8CFU, protease activity is not less than 200U, and amylase content is not less than 100U.
The preparation method of described microorganism renovation agent of water environment, this method cultivate each component microorganism respectively to be mixed and made into microorganism renovation agent more by a certain percentage.
The preparation method of described microorganism renovation agent of water environment comprises the steps:
A. the preparation of primary seed solution: respectively with activatory subtilis, Bacillus licheniformis, denitrification Alcaligenes bacterial classification inoculation in the nutrient broth of aseptic pH7.0～7.5, actinomycetes are seeded in the starch culture medium of sterilization, 30～37 ℃, 150～200rpm, incubated overnight; Photosynthetic bacterium is in the photosynthetic bacterium liquid nutrient medium, and 30～37 ℃, illumination cultivation 4-7 days, intensity of illumination 1000-2000Lx obtained the primary seed solution of each bacterial classification;
B. secondary seed solution fermentation: the subtilis that step a is cultivated, Bacillus licheniformis, denitrification Alcaligenes, actinomycetes primary seed solution insert fermentor tank by the inoculum size of fermentor tank volume 10～15% respectively, substratum is used with step a, 30～37 ℃, 150～200rpm, feeding is through the filtrated air of 0.45 μ m membrane filtration, air flow is fermentor tank volume 0.5-1 times/min, and pressure 0.1～1MPa fermented 12～18 hours;
C. produce the liquid fermentation: respectively secondary seed solution is squeezed in the fermentor tank, substratum is used with step a, inoculum size is the 8-12% of fermentor tank volume, 30～37 ℃, 150～200rpm feeds the filtrated air through 0.45 μ m membrane filtration, and air flow is fermentor tank volume 0.8-1 times/min, pressure fermented 24～36 hours at 0.5～1.0MPa; Photosynthetic bacterium directly is inoculated into primary seed solution in the transparent fermentor tank that the photosynthetic bacterium liquid nutrient medium is housed, and inoculum size is the 8-12% of fermentor tank volume, and 30～37 ℃, illumination cultivation 4-7 days, intensity of illumination 1000-2000Lx;
D. the receiver of after separately fermentation is finished fermented liquid being packed into, get subtilis 30%～40% according to the mass percent of each component microorganism in the microbial total quality, Bacillus licheniformis 30%～40%, actinomycetes 10～20%, denitrifying bacterium 10～20%, photosynthetic bacterium 10～20% is mixed and made into the liquid microbe renovation agent; Perhaps
With the fermented liquid of fermentation separately by centrifugal or add carrier absorption, form solid, get subtilis 30%～40% according to the mass percent of each component microorganism in the microbial total quality, Bacillus licheniformis 30%～40%, actinomycetes 10～20%, denitrifying bacterium 10～20%, photosynthetic bacterium 10～20% is mixed and made into the solid microbe renovation agent.
The preparation method of described microorganism renovation agent of water environment, the bacterial content of liquid microbe renovation agent is not less than 5.0 * 10 in the steps d
8CFU/ml, protease activity is not less than 200U/ml, and amylase content is not less than 100U/ml; The bacterial content of solid microbe renovation agent is not less than 5.0 * 10
8CFU/g, protease activity is not less than 200U/g, and amylase content is not less than 100U/g.
Beneficial effect of the present invention:
The microorganism water surrounding renovation agent that adopts the present invention to obtain is that biological improver of water quality has been compared following advantage with tradition:
1) employed microorganism can produce bioactive enzyme
Can produce proteolytic enzyme, diastatic bacterial strain purpose bacterium by modern microbe separation technology, Protocols in Molecular Biology and enzyme engineering technology screening as microorganism renovation agent of water environment, these bacterium can produce a large amount of proteolytic enzyme, amylase, the content of proteolytic enzyme is not less than 200U/g or 200U/ml, and amylase is not less than 100U/g or 100U/ml.These endonuclease capables become available small-molecule substance with protein, the starch degradation that is not digested by animal in residual bait in the water and the ight soil effectively, quicken the degraded of residual bait, ight soil.
2) employed microorganism is based on water surrounding ancestral home bacterium
Microorganism has certain environmental compatibility as all biologies, the used microorganism great majority of traditional improver of water quality are Lu Sheng, arrive water body and can not adapt to water surrounding, therefore can not very fast breeding, form dominant microflora, and the ancestral home bacterium is isolating from water surrounding, and is strong to water surrounding adaptability, and artificial the input can form dominant microflora rapidly later.
3) employed microorganism can also produce microbiotic sample material
Selected microorganism such as genus bacillus, actinomycetes can produce microbiotic sample material, and pathogenic bacterium are had restraining effect, thereby prevent the generation of hydrocoles disease effectively, for the fishery products of producing green safety escort.
Strain characteristic and function that the present invention is selected are as follows:
1) subtilis can produce proteolytic enzyme, amylase and subtilin,
2) Bacillus licheniformis can produce proteolytic enzyme, bacitracin etc.,
3) actinomycetes, the long-living microbiotic sample material of energy,
4) denitrification Alcaligenes, the nitrite in the water body of effectively degrading,
5) photosynthetic bacterium, objectionable impuritiess such as the ammonia nitrogen in the water body of effectively degrading, hydrogen sulfide.
Embodiment
The invention will be further elaborated by the following examples.
One, activation medium:
1, nutrient broth agar substratum: contain peptone 10g in every liter of substratum, beef extract 3.0g, sodium-chlor 5.0g, agar 20g, pH7.0～7.5.Sterilization.
2, Starch Agar substratum: every liter of substratum soluble-containing starch 20 grams, saltpetre 1.0g, sodium-chlor 0.5g, dipotassium hydrogen phosphate 0.5g, sal epsom 0.5g, ferrous sulfate 0.01g, agar 20g, pH7.2.Sterilization.
3, photosynthetic bacterium solid medium: every liter of substratum sulfur acid ammonium 1g, magnesium sulfate heptahydrate 0.2g, sodium bicarbonate 5g, dipotassium hydrogen phosphate 0.5g, peptone 2g, agar 20g, pH7.5.Sterilization.
Two, liquid nutrient medium: the same activation medium of filling a prescription, but do not contain agar.
1, nutrient broth medium: contain peptone 10g in every liter of substratum, beef extract 3.0g, sodium-chlor 5.0g, pH7.0～7.5.Sterilization.
2, starch culture-medium: every liter of substratum soluble-containing starch 20 grams, saltpetre 1.0g, sodium-chlor 0.5g, dipotassium hydrogen phosphate 0.5g, sal epsom 0.5g, ferrous sulfate 0.01g, pH7.2.Sterilization.
3, photosynthetic bacteria culture medium: every liter of substratum sulfur acid ammonium 1g, magnesium sulfate heptahydrate 0.2g, sodium bicarbonate 5g, dipotassium hydrogen phosphate 0.5g, peptone 2g, pH7.5.Sterilization.
The bacterial classification of freeze-drying or 4 ℃ of preservations is carried out slant activation, on the original genus bacillus bacterioid preserved, the denitrification Alcaligenes bacterial classification inoculation nutrient broth agar flat board to about aseptic pH7.0～7.5, actinomycetes are seeded in the Zulkovsky starch nutrient agar of sterilization; Photosynthetic bacterium in the photosynthetic bacterium solid medium, 30～37 ℃, illumination cultivation 4～7 days.
Embodiment 1
1) bacterial classification with freeze-drying lactobacillus or 4 ℃ of preservations carries out slant activation.With subtilis CGMCC 1.504, Bacillus licheniformis CGMCC1.105, denitrification Alcaligenes CGMCC1.768 are seeded to the nutrient broth agar substratum and carry out slant activation respectively; Actinomycetes CGMCC4.1040 is seeded to the Starch Agar substratum and carries out slant activation; Photosynthetic bacterium CGMCC4.1187 is seeded to the photosynthetic bacterium solid medium and carries out slant activation.
2) preparation of primary seed solution: picking colonies typical from the activatory agar plate, respectively with subtilis CGMCC 1.504, Bacillus licheniformis CGMCC1.105, denitrification Alcaligenes CGMCC1.768 is inoculated in the nutrient broth of aseptic pH7.0, actinomycetes CGMCC4.1040 is seeded in the starch culture medium of sterilization, 35 ℃, 150rpm, incubated overnight; Photosynthetic bacterium CGMCC4.1187 is inoculated in the photosynthetic bacterium liquid nutrient medium, and 35 ℃, illumination cultivation 4 days, intensity of illumination 1500Lx; Obtain the primary seed solution of each bacterial classification.
3) secondary seed solution fermentation: respectively with step 2) the subtilis CGMCC 1.504 that is cultivated, Bacillus licheniformis CGMCC1.105, the inoculum size that denitrification Alcaligenes CGMCC1.768, actinomycetes CGMCC4.1040 primary seed solution are pressed fermentor tank volume 10% inserts the 100L fermentor tank, the substratum step is with 1) used, 37 ℃, 150rpm, feeding is through the filtrated air of 0.45 μ m membrane filtration, air flow is fermentor tank volume 0.5-1 times/min, and pressure fermented 12 hours at 0.1MPa;
4) produce the liquid fermentation: respectively secondary seed solution is squeezed in 1 ton of fermentor tank, substratum is with step 2) used, inoculum size is 10% of a fermentor tank volume, 37 ℃, 150rpm feeds the filtrated air through 0.45 μ m membrane filtration, and air flow is fermentor tank volume 0.8-1 times/min, pressure fermented 36 hours at 0.5～1.0MPa.Photosynthetic bacterium directly is inoculated into primary seed solution in the transparent container that the photosynthetic bacterium liquid nutrient medium is housed, and inoculum size is 10%, 35 ℃ of fermentor tank volume, illumination cultivation 7 days, intensity of illumination 2000Lx.
5) receiver of after separately fermentation is finished fermented liquid being packed into, get subtilis 40% according to the mass percent of each component microorganism in the microbial total quality, Bacillus licheniformis 30%, actinomycetes 10%, photosynthetic bacterium 10%, denitrification Alcaligenes 10% are mixed and made into the liquid microbe renovation agent.After measured, bacterial content is not less than 5.0 * 10
8CFU/ml, protease activity is not less than 1000U/ml, and amylase content is not less than 100U/ml.
Embodiment 2
Follow these steps to carry out:
1) bacterial classification with freeze-drying lactobacillus or 4 ℃ of preservations carries out slant activation.Subtilis CGMCC1.504, Bacillus licheniformis CGMCC1.105, denitrification Alcaligenes CGMCC1.768 carries out slant activation with the nutrient broth agar substratum; Actinomycetes CGMCC4.1040 carries out slant activation with the Starch Agar substratum; Photosynthetic bacterium CGMCC4.1187 carries out slant activation with photosynthetic bacterium solid culture medium.
2) preparation of primary seed solution.Picking colonies typical from the activatory agar plate, respectively with subtilis CGMCC 1.504, Bacillus licheniformis CGMCC1.105, denitrification Alcaligenes CGMCC1.768 is inoculated in the nutrient broth of aseptic pH7.5, actinomycetes CGMCC4.1040 is seeded in the starch culture medium of sterilization, 37 ℃, 200rpm, incubated overnight; Photosynthetic bacterium CGMCC4.1187 is seeded in the photosynthetic bacterium liquid nutrient medium, and 37 ℃, illumination cultivation 7 days, intensity of illumination 2000Lx; Obtain the primary seed solution of each bacterial classification.
3) secondary seed solution fermentation.With step 2) sporeformer cultivated, actinomycetes, denitrification Alcaligenes primary seed solution insert the 100L fermentor tank by the inoculum size of fermentor tank volume 15%, substratum is used with step 1), 37 ℃, 200rpm, feeding is through the filtrated air of 0.45 μ m membrane filtration, air flow is fermentor tank volume 0.5-1 times/min, and pressure fermented 16 hours at 0.8MPa.
4) produce the liquid fermentation.Secondary seed solution is squeezed in 1 ton of fermentor tank, and substratum is with 2), 10%, 37 ℃ of inoculum size, 200rpm feeds the filtrated air through 0.45 μ m membrane filtration, and air flow is fermentor tank volume 0.8-1 times/min, and pressure fermented 30 hours at 0.8MPa.Photosynthetic bacterium directly is inoculated into primary seed solution in the transparent container that the photosynthetic bacterium liquid nutrient medium is housed, and 37 ℃, illumination cultivation 7 days, intensity of illumination 2000Lx.
5) with the pure thalline of the centrifugal acquisition of fermented liquid, get subtilis 30% according to the mass percent of each component microorganism in the microbial total quality, Bacillus licheniformis 30%, actinomycetes 20%, the mixed of photosynthetic bacterium 10%, denitrification Alcaligenes 10% is made the solid microbe renovation agent with zeolite powder as carrier.After measured, bacterial content is not less than 5.0 * 10
8CFU/g, protease activity is not less than 1000U/g, and amylase content is not less than 100U/g.
Embodiment 3
Adopt the microorganism renovation agent of water environment that contains biological enzyme of embodiment of the invention preparation, according to 400～500ml/ mu or 400～500g/ mu (per 2 week once), in aquaculture eutrophication and other organic ponds of polluting, use, the objectionable impuritiess such as residual bait in the pond, ight soil, animals and plants corpse and ammonia nitrogen, hydrogen sulfide, nitrite of can effectively degrading, effectively purify water, improve the aquaculture water environment, suppress the growth of patient bacterium, for the production of green aquatic product escorts.
1, materials and methods
1.1 test materials: the Chinese prawn juvenile prawn is all available from the plant of healthy aquaculture, for a collection of breeding, and the specification unanimity.Microorganism renovation agent makes as stated above.
1.2 test method before on-test, selects 4 sizes to be 10 mu the shrimp pool in Jiangsu plant.Put in order, clean up the pond, 1 week lime disinfection was carried out on 4 pools in advance, discharge water then and cultivate food organism.Wherein the 1st, 2 are the test pool, and the 3rd, 4 are the contrast pool, and breeding density is 7500 tail/mus.Microbial preparation is brought into use on the test pool from May 10th, 2007, per 2 weeks are used once, and each usage quantity is 400～500ml/ mu (adopting solid microbe renovation agent usage quantity is 400～500g/ mu).4 pond culture management conditions are in full accord, and whole breeding process is without other any medicine.
The pool under the juvenile prawn, every day at the upper and lower noon is measured pH value, dissolved oxygen amount, water temperature, water colour and the daily ration, feeding quantity of 4 pond waters, observes the behavior of prawn.Afternoon, 3:00 measured ammonia nitrogen, nitrite, the hydrogen sulfide content in the water one time weekly.The intelligent Water Test Kits of MR220 that the water quality physical and chemical index adopts Nanjing Te An scientific ﹠ trading Co., Ltd. to produce is measured.Test from May 10th, 2007 to July 9,60d altogether.
2, result and analysis
2.1 the comparison of ammonia-nitrogen content: a little higher than test group of the ammonia nitrogen of control group, the average of test group ammonia nitrogen is 0.52mg/L during the whole test, and control group is 0.85mg/L, two groups of difference is significantly (P＞0.05) not.The fluctuating range of control group is big (standard deviation is 0.68), and the fluctuating range of test group less relatively (standard deviation is 0.35) shows that this probiotics can make the ammonia nitrogen in the water maintain a metastable state.
2.2 the comparison of nitrite content: the average nitrite content of test group is 0.03 ± 0.01mg/L, is starkly lower than control group 0.07 ± 0.01mg/L, and difference is (P＜0.01) extremely significantly.Illustrate that this probiotics can extremely significantly reduce the content of nitrite in the water.
2.3 the comparison of hydrogen sulfide content: the hydrogen sulfide content of control group is higher than test group, and fluctuating range is bigger.The average content of test group hydrogen sulfide is 0.12 ± 0.04mg/L in the whole process, and control group is 0.23 ± 0.08mg/L, significant difference (P＜0.05).Show that probiotics plays remarkable effect to the hydrogen sulfide that reduces in the water.
3, conclusion
From this research experiment result as can be seen, the prepared in this way microorganism water surrounding renovation agent that contains biological enzyme can obviously reduce content of harmful such as nitrite in the water and hydrogen sulfide, and ammonia nitrogen is had certain reduction effect, but not obvious.Therefore we can say that this biotechnological formulation can improve the raising pond water quality effectively, favourable to stablizing of the little ecologic structure of aquaculture water.
Claims (5)
1, a kind of microorganism renovation agent of water environment, it is characterized in that the mass percent of each component microorganism of this renovation agent in the microbial total quality is: subtilis 30%～40%, Bacillus licheniformis 30%～40%, actinomycetes 10～20%, denitrifying bacterium 10～20%, photosynthetic bacterium 10～20%.
2, microorganism renovation agent of water environment according to claim 1 is characterized in that bacterial content is not less than 5.0 * 10 in every gram or this microorganism renovation agent of every m1
8CFU, protease activity is not less than 200U, and amylase content is not less than 100U.
3, the preparation method of microorganism renovation agent of water environment as claimed in claim 1 or 2 is characterized in that this method is each component microorganism to be cultivated respectively be mixed and made into microorganism renovation agent more by a certain percentage.
4, the preparation method of microorganism renovation agent of water environment according to claim 3 is characterized in that it comprises the steps:
A. the preparation of primary seed solution: respectively with activatory subtilis, Bacillus licheniformis, denitrification Alcaligenes bacterial classification inoculation in the nutrient broth of aseptic pH7.0～7.5, actinomycetes are seeded in the starch culture medium of sterilization, 30～37 ℃, 150～200rpm, incubated overnight; Photosynthetic bacterium in the photosynthetic bacterium liquid nutrient medium, 30～37 ℃, illumination cultivation 4-7 days, intensity of illumination 1000-2000Lx; Obtain the primary seed solution of each bacterial classification;
B. secondary seed solution fermentation: the subtilis that step a is cultivated, Bacillus licheniformis, denitrification Alcaligenes, actinomycetes primary seed solution insert fermentor tank by the inoculum size of fermentor tank volume 10～15% respectively, substratum is used with step a, 30～37 ℃, 150～200rpm, feeding is through the filtrated air of 0.45 μ m membrane filtration, air flow is fermentor tank volume 0.5-1 times/min, and pressure 0.1～1MPa fermented 12～18 hours;
C. produce the liquid fermentation: respectively secondary seed solution is squeezed in the fermentor tank, substratum is used with step a, inoculum size is the 8-12% of fermentor tank volume, 30～37 ℃, 150～200rpm feeds the filtrated air through 0.45 μ m membrane filtration, and air flow is fermentor tank volume 0.8-1 times/min, pressure fermented 24～36 hours at 0.5～1.0MPa; Photosynthetic bacterium directly is inoculated into primary seed solution in the transparent fermentor tank that the photosynthetic bacterium liquid nutrient medium is housed, and inoculum size is the 8-12% of fermentor tank volume, and 30～37 ℃, illumination cultivation 4-7 days, intensity of illumination 1000-2000Lx;
D. the receiver of after separately fermentation is finished fermented liquid being packed into, get subtilis 30%～40% according to the mass percent of each component microorganism in the microbial total quality, Bacillus licheniformis 30%～40%, actinomycetes 10～20%, denitrifying bacterium 10～20%, photosynthetic bacterium 10～20% is mixed and made into the liquid microbe renovation agent; Perhaps
With the fermented liquid of fermentation separately by centrifugal or add carrier absorption, form solid, get subtilis 30%～40% according to the mass percent of each component microorganism in the microbial total quality, Bacillus licheniformis 30%～40%, actinomycetes 10～20%, denitrifying bacterium 10～20%, photosynthetic bacterium 10～20% is mixed and made into the solid microbe renovation agent.
5, preparation method according to claim 4 is characterized in that the bacterial content of liquid microbe renovation agent in the steps d is not less than 5.0 * 10
8CFU/ml, protease activity is not less than 200U/ml, and amylase content is not less than 100U/ml; The bacterial content of solid microbe renovation agent is not less than 5.0 * 10
8CFU/g, protease activity is not less than 200U/g, and amylase content is not less than 100U/g.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN200810025135A CN100588624C (en) | 2008-05-08 | 2008-05-08 | Microorganism renovation agent of water environment and preparation method thereof |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN200810025135A CN100588624C (en) | 2008-05-08 | 2008-05-08 | Microorganism renovation agent of water environment and preparation method thereof |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN101269884A true CN101269884A (en) | 2008-09-24 |
| CN100588624C CN100588624C (en) | 2010-02-10 |
Family
ID=40004146
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN200810025135A Expired - Fee Related CN100588624C (en) | 2008-05-08 | 2008-05-08 | Microorganism renovation agent of water environment and preparation method thereof |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN100588624C (en) |
Cited By (18)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102531198A (en) * | 2012-01-09 | 2012-07-04 | 信息产业电子第十一设计研究院科技工程股份有限公司 | Water body cleansing agent |
| CN102583776A (en) * | 2012-02-29 | 2012-07-18 | 中国水产科学研究院淡水渔业研究中心 | Compound bacteria enzyme preparation for improving cultivation watery environment and preparation method |
| CN102649603A (en) * | 2011-02-24 | 2012-08-29 | 上海三智生物科技有限公司 | High-activity and high-efficiency solid biological substrate-improving granule |
| CN102765815A (en) * | 2012-07-05 | 2012-11-07 | 昆明豪原特自控有限公司 | Biological decomposer and preparation method thereof |
| CN102815792A (en) * | 2012-04-01 | 2012-12-12 | 浙江绿凯环保科技有限公司 | Microbe-enzyme composite preparation used for restoring water in urban and rural polluted river, and its preparation method |
| CN102992493A (en) * | 2012-10-22 | 2013-03-27 | 北京嘉禾动物营养科技有限公司 | Improved combined microbe repairing agent for aquaculture environment regulation |
| CN103395890A (en) * | 2013-07-19 | 2013-11-20 | 上海创博生态工程有限公司 | Microbial preparation for improving freshwater aquaculture water body and preparation method thereof |
| CN103409320A (en) * | 2013-07-01 | 2013-11-27 | 青岛浩澳环保科技有限公司 | Flora microbe purification agent as well as preparation and application methods thereof |
| CN103409338A (en) * | 2013-07-04 | 2013-11-27 | 大连施倍得生物技术有限公司 | Microbial water quality purifying agent for aquaculture pond and preparation method and strain curing method thereof |
| CN103898002A (en) * | 2012-12-31 | 2014-07-02 | 都江堰惠农生物技术有限责任公司 | Method for producing compound bacteria for water environment improvement |
| CN103923849A (en) * | 2013-01-10 | 2014-07-16 | 都江堰惠农生物技术有限责任公司 | Production method of compound bacteria for removing stink from livestock and poultry culturing farm |
| CN104862298A (en) * | 2015-04-23 | 2015-08-26 | 陆少英 | Composite microbial culture starter and preparation method thereof |
| CN105254026A (en) * | 2015-09-30 | 2016-01-20 | 胡小勇 | Application of amaranth forage parcel in water purification |
| CN105753272A (en) * | 2016-05-14 | 2016-07-13 | 王浩丞 | Sewage treatment method for garden planning |
| CN105981637A (en) * | 2015-02-13 | 2016-10-05 | 上海太和水环境科技发展有限公司 | Method of adding microorganisms for promoting growth of common andrographis herbs |
| CN107235620A (en) * | 2017-05-22 | 2017-10-10 | 安徽省通源环境节能股份有限公司 | A kind of long-acting comprehensive sediment repairing agent and preparation method thereof |
| CN107673481A (en) * | 2017-09-29 | 2018-02-09 | 乔银娣 | A kind of water body purification of aquaculture agent and preparation method thereof |
| CN109321489A (en) * | 2018-09-28 | 2019-02-12 | 邓州市汇海农业科技有限公司 | A kind of rice shrimp mixed breeding field complex microbial inoculum |
-
2008
- 2008-05-08 CN CN200810025135A patent/CN100588624C/en not_active Expired - Fee Related
Cited By (22)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102649603A (en) * | 2011-02-24 | 2012-08-29 | 上海三智生物科技有限公司 | High-activity and high-efficiency solid biological substrate-improving granule |
| CN102531198A (en) * | 2012-01-09 | 2012-07-04 | 信息产业电子第十一设计研究院科技工程股份有限公司 | Water body cleansing agent |
| CN102583776A (en) * | 2012-02-29 | 2012-07-18 | 中国水产科学研究院淡水渔业研究中心 | Compound bacteria enzyme preparation for improving cultivation watery environment and preparation method |
| CN102583776B (en) * | 2012-02-29 | 2013-04-24 | 中国水产科学研究院淡水渔业研究中心 | Compound bacteria enzyme preparation for improving cultivation watery environment and preparation method |
| CN102815792A (en) * | 2012-04-01 | 2012-12-12 | 浙江绿凯环保科技有限公司 | Microbe-enzyme composite preparation used for restoring water in urban and rural polluted river, and its preparation method |
| CN102765815A (en) * | 2012-07-05 | 2012-11-07 | 昆明豪原特自控有限公司 | Biological decomposer and preparation method thereof |
| CN102992493A (en) * | 2012-10-22 | 2013-03-27 | 北京嘉禾动物营养科技有限公司 | Improved combined microbe repairing agent for aquaculture environment regulation |
| CN103898002A (en) * | 2012-12-31 | 2014-07-02 | 都江堰惠农生物技术有限责任公司 | Method for producing compound bacteria for water environment improvement |
| CN103923849A (en) * | 2013-01-10 | 2014-07-16 | 都江堰惠农生物技术有限责任公司 | Production method of compound bacteria for removing stink from livestock and poultry culturing farm |
| CN103409320A (en) * | 2013-07-01 | 2013-11-27 | 青岛浩澳环保科技有限公司 | Flora microbe purification agent as well as preparation and application methods thereof |
| CN103409338A (en) * | 2013-07-04 | 2013-11-27 | 大连施倍得生物技术有限公司 | Microbial water quality purifying agent for aquaculture pond and preparation method and strain curing method thereof |
| CN103395890A (en) * | 2013-07-19 | 2013-11-20 | 上海创博生态工程有限公司 | Microbial preparation for improving freshwater aquaculture water body and preparation method thereof |
| CN103395890B (en) * | 2013-07-19 | 2015-06-03 | 上海创博生态工程有限公司 | Microbial preparation for improving freshwater aquaculture water body and preparation method thereof |
| CN105981637A (en) * | 2015-02-13 | 2016-10-05 | 上海太和水环境科技发展有限公司 | Method of adding microorganisms for promoting growth of common andrographis herbs |
| CN104862298A (en) * | 2015-04-23 | 2015-08-26 | 陆少英 | Composite microbial culture starter and preparation method thereof |
| CN105254026A (en) * | 2015-09-30 | 2016-01-20 | 胡小勇 | Application of amaranth forage parcel in water purification |
| CN105753272A (en) * | 2016-05-14 | 2016-07-13 | 王浩丞 | Sewage treatment method for garden planning |
| CN105753272B (en) * | 2016-05-14 | 2018-11-16 | 王浩丞 | Sewage water treatment method in a kind of Garden Planning |
| CN107235620A (en) * | 2017-05-22 | 2017-10-10 | 安徽省通源环境节能股份有限公司 | A kind of long-acting comprehensive sediment repairing agent and preparation method thereof |
| CN107673481A (en) * | 2017-09-29 | 2018-02-09 | 乔银娣 | A kind of water body purification of aquaculture agent and preparation method thereof |
| CN107673481B (en) * | 2017-09-29 | 2021-05-07 | 江苏好润生物产业集团股份有限公司 | Aquaculture water body purifying agent and preparation method thereof |
| CN109321489A (en) * | 2018-09-28 | 2019-02-12 | 邓州市汇海农业科技有限公司 | A kind of rice shrimp mixed breeding field complex microbial inoculum |
Also Published As
| Publication number | Publication date |
|---|---|
| CN100588624C (en) | 2010-02-10 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN100588624C (en) | Microorganism renovation agent of water environment and preparation method thereof | |
| CN102924134B (en) | Alga microorganism fertilizer agent and preparation method thereof | |
| CN101690545A (en) | Method for producing complex micro-ecological preparation with microbial agents and enzyme | |
| CN101974434B (en) | Multi-bacterium microbial compound preparations for aquaculture and preparation method thereof | |
| CN107541477B (en) | Method for culturing photosynthetic bacteria by using lactobacillus fermentation liquor | |
| CN102220262A (en) | Composite microbiological preparation and preparation method and use thereof | |
| CN103184174B (en) | Production method of bacillus subtilis biological agent used for sodium humate-containing feed in medium | |
| CN102719380B (en) | Aquatic compound microecologics and preparation method thereof | |
| CN106754551A (en) | A kind of bacterium amount lactobacillus preparation high and preparation method and application | |
| CN101921710B (en) | Repairing agent for microbes in water bodies of excessive culture zones | |
| CN111793575A (en) | Complex microbial inoculant and application thereof in aquaculture | |
| CN109607819A (en) | A kind of control of microorganisms aquaculture method | |
| CN101624234B (en) | Microorganism water treatment agent in culturing water and preparation technique thereof | |
| CN114921385A (en) | Bacillus subtilis and application thereof in feed addition and antibiotic-free culture | |
| CN104593303A (en) | Liquid composite microbial agent and production method thereof | |
| CN111172058B (en) | Bacillus amyloliquefaciens and application thereof | |
| CN103740615B (en) | Photosynthetic bacteria SC01 and fast culture process thereof and application | |
| CN108423838B (en) | Microbial preparation for ecological safety water system and preparation method thereof | |
| KR20140074451A (en) | Microbial agent for improving water quality of aquarium | |
| CN109055264A (en) | A kind of microbial bacterial agent and preparation method thereof for holothruian cultures | |
| CN103898002A (en) | Method for producing compound bacteria for water environment improvement | |
| CN101270335A (en) | Multipurpose microorganism additive agent | |
| CN114437975B (en) | Lactic acid-producing bacillus coagulans and application thereof | |
| JP2013132248A (en) | Method for culturing photosynthetic bacterium and photosynthetic bacterium | |
| CN113213642B (en) | Water-regulating microecological preparation for micropterus salmoides seedling culture and preparation method thereof |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant | ||
| ASS | Succession or assignment of patent right |
Free format text: FORMER OWNER: JIANGSU NUHIGH BIOTECHNOLOGY CO., LTD. Effective date: 20130412 Owner name: JIANGSU NUHIGH BIOTECHNOLOGY CO., LTD. Free format text: FORMER OWNER: CHU WEIHUA Effective date: 20130412 |
|
| C41 | Transfer of patent application or patent right or utility model | ||
| C56 | Change in the name or address of the patentee | ||
| COR | Change of bibliographic data |
Free format text: CORRECT: ADDRESS; FROM: 210014 NANJING, JIANGSU PROVINCE TO: 224233 YANCHENG, JIANGSU PROVINCE |
|
| CP01 | Change in the name or title of a patent holder |
Address after: Xuanwu District of Nanjing City, Jiangsu province 210014 Wei Tong Road No. 16 room 3-106 Patentee after: Chu Weihua Patentee after: JIANGSU XINGHAI BIOLOGICAL TECHNOLOGY CO.,LTD. Address before: Xuanwu District of Nanjing City, Jiangsu province 210014 Wei Tong Road No. 16 room 3-106 Patentee before: Chu Weihua Patentee before: JIANGSU XINGHAI ANIMAL MEDICINE TECHNOLOGY Co.,Ltd. |
|
| TR01 | Transfer of patent right |
Effective date of registration: 20130412 Address after: 224233 Tang Yang Science Park, Dongtai, Jiangsu Patentee after: JIANGSU XINGHAI BIOLOGICAL TECHNOLOGY CO.,LTD. Address before: Xuanwu District of Nanjing City, Jiangsu province 210014 Wei Tong Road No. 16 room 3-106 Patentee before: Chu Weihua Patentee before: JIANGSU XINGHAI BIOLOGICAL TECHNOLOGY CO.,LTD. |
|
| ASS | Succession or assignment of patent right |
Owner name: YANCHENG XINGHAI FEED CO., LTD. Free format text: FORMER OWNER: JIANGSU NUHIGH BIOTECHNOLOGY CO., LTD. Effective date: 20140310 |
|
| C41 | Transfer of patent application or patent right or utility model | ||
| TR01 | Transfer of patent right |
Effective date of registration: 20140310 Address after: 224233 Tang Yang Science Park, Yancheng City, Jiangsu, Dongtai Patentee after: YANCHENG XINGHAI FEED Co.,Ltd. Address before: 224233 Tang Yang Science Park, Dongtai, Jiangsu Patentee before: JIANGSU XINGHAI BIOLOGICAL TECHNOLOGY CO.,LTD. |
|
| CF01 | Termination of patent right due to non-payment of annual fee | ||
| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20100210 Termination date: 20170508 |