CN105254026A - Application of amaranth forage parcel in water purification - Google Patents
Application of amaranth forage parcel in water purification Download PDFInfo
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- CN105254026A CN105254026A CN201510645260.9A CN201510645260A CN105254026A CN 105254026 A CN105254026 A CN 105254026A CN 201510645260 A CN201510645260 A CN 201510645260A CN 105254026 A CN105254026 A CN 105254026A
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Abstract
The invention discloses the application of an amaranth forage parcel in water purification. The amaranth forage parcel is prepared according to the steps of smashing and pre-drying harvested amaranth, then spraying a composite microbial preparation on amaranth to enable amaranth to propagate massively and be gathered in forage, conducting fermentation treatment under the anaerobic condition, conducting bundling and parcel wrapping, and finally obtaining the amaranth forage parcel. By loading microorganisms in amaranth and conducting fermentation treatment, amaranth and microorganisms in the prepared amaranth forage parcel can purify water together, high efficiency, durability and low cost are realized, and environment safety is high.
Description
Technical field
The invention belongs to technical field of water body purification, be specifically related to amaranth seed powder forage and wrap up in the application wrapped in purifying water body.
Background technology
It is one of problem of environmental pollution of current social growing interest that lake pollution is gushed in river.Because the activity of the mankind is intensive, the irregular discharge of pollutent, causes the severe contamination in river.Lake is gushed in the random river that is discharged into of domestic refuse and sanitary sewage, causes water body fishes and shrimps to disappear, nutritive salt enrichment, finally occurs water quality deterioration, black smelly, the ecological balanced system of havoc water body, makes river course lose self-purification function.The factor that lake treatment impact is gushed in river is a lot of also very complicated, and the cycle of administering is long, difficulty is large, has also become the key subjects of domestic and international environment specialist's research.
The traditional method that lake pollution is gushed in current domestic process river has Physical, as mechanical filter, Dredged bed mud, regularly moisturizing etc.; Chemical method, as thrown in copper sulfate and chlorinated lime; Current various treatment technology all also exists certain limitation: Physical cost is high, cures the symptoms, not the disease; Chemical method introduces new chemical composition, causes secondary pollution to ecotope; Biological process starts from the seventies in last century, is developed rapidly in the nineties.Countries in Europe just carries out preliminary study to biological process from the mid-80 in last century, and completes some actual treatment engineerings.The U.S. also comes into effect the administration project of the environmental pollutant such as underground water the nineties.But there is bacterial screening complex process, microorganism to shortcomings such as water body selectivity instability in foreign biomolecule method.To the development research of the microbiological treatment technology of efficient, non-secondary pollution, it is the development trend of water surrounding ecological reestablishment.
Therefore, need to develop a kind of microbiological water purifying technology efficient, lasting, with low cost.
Summary of the invention
Technical problem to be solved by this invention is to provide amaranth seed powder forage and wraps up in the application wrapped in purifying water body.
Above-mentioned technical problem to be solved by this invention is achieved through the following technical solutions: amaranth seed powder forage wraps up in the application wrapped in purifying water body.
Wherein, described amaranth seed powder forage is wrapped up in bag and is prepared by the following method: by the amaranth seed powder of harvesting after comprising pulverizing and predrying operation process, amaranth seed powder sprays complex microorganism preparations, make its amount reproduction and be gathered in forage, under anaerobic carry out fermentative processing, again after comprising bundling and wrapping up in the process of job contract sequence, finally make amaranth seed powder forage and wrap up in bag.
Complex microorganism preparations in the present invention, is made up of fermentation of bacillus liquid, nitrobacteria fermented liquid and Fermentation by Photosynthetic Bacteria liquid, and the consumption of this complex microorganism preparations is account for amaranth seed powder total mass 8% ~ 12%.
The mass percentage of fermentation of bacillus liquid, nitrobacteria fermented liquid and Fermentation by Photosynthetic Bacteria liquid three is respectively 30 ~ 40%, 20 ~ 30% and 30 ~ 50%.
This complex microorganism preparations has high reactivity and organics removal ability.Wherein genus bacillus, nitrobacteria and photosynthetic bacterium are commercially available bacterial classification.
The preparation method of fermentation of bacillus liquid, nitrobacteria fermented liquid and Fermentation by Photosynthetic Bacteria liquid is: get genus bacillus, nitrobacteria and photosynthetic bacterium bacterial classification respectively, by its specification sheets or ordinary method activated, enlarged culturing process respectively, being prepared into concentration is respectively 1 × 10
7~ 1 × 10
8the genus bacillus kind liquid of individual/g, nitrobacteria kind liquid or photosynthetic bacterium kind liquid, by genus bacillus kind liquid, nitrobacteria kind liquid, photosynthetic bacterium kind liquid inoculation fermentation on respective substratum respectively, fermentation of bacillus liquid, nitrobacteria fermented liquid or Fermentation by Photosynthetic Bacteria liquid can be obtained.
Wherein genus bacillus kind liquid can adopt broth agar culture medium, beef-protein medium, potato dextrose agar etc.
Nitrobacteria kind liquid can adopt culture medium prescription 1 to be: peptone: 1%, yeast extract paste: 0.5%, sodium-chlor: 1%, agar: 2%, distilled water: 100mL, pH value: 7.2 ~ 7.5; Employing culture medium prescription 2 is: potato: 20%, peptone: 0.5%, white sugar: 1%, glucose: 1%, mixed inorganic: 1%, agar: 2%, tap water: 100mL, pH:7.0, and employing culture medium prescription 3 is: peptone: 1%, extractum carnis: 0.3%, sodium-chlor: 0.5%, agar: 2%, distilled water: 100mL, pH value: 7.0 etc.
The conventional liquid culture based formulas that photosynthetic bacterium can adopt is: NH
4cl1.0g, CH
3cOONa3.5g, MgC1
20.1g, CaC1
20.1g, KH
2pO
40.6g, K
2hPO
40.4g, yeast extract paste 0.1g, water 1000mL, pH7.2.
Under anaerobic carry out, complex microorganism preparations is sprayed in amaranth seed powder, in 20 ~ 40 DEG C of fermentations process in 20 ~ 30 days, then after comprising bundling and wrapping up in the process of job contract sequence, finally make amaranth seed powder forage and wrap up in bag.
The amaranth seed powder forage made is wrapped up in after bag storage carries out continuation fermentative processing in 40 ~ 60 days and namely be can be used for purifying water body.
During by the amaranth seed powder of harvesting through comprising pulverizing process process, the length that preferred powder is broken to amaranth seed powder is preferably 1.0 ~ 1.5cm, and broken joint rate is preferably more than 75%.
During by the amaranth seed powder of harvesting through comprising predrying operation process, preferably the predrying mass percentage to moisture in amaranth seed powder is 65 ~ 75%.
In the present invention, amaranth seed powder can be the food of the biologies such as water body Mesichthyes, it can be again the carrier of complex microorganism, play an important role in water body purification, wherein complex microorganism plays the keying action of purifying water body, its with Eutrophication materials such as the phosphorus in water body, salt for bait, and rapidly breeding increases protozoon and metazoan, thus the pollution substance in removing water body, these complex microorganisms are again the high-quality food materials of the organisms in water such as fish, thus desirable food chain can be formed in water, reach the object of purifying water body.
The water body that water body in the present invention can to gush etc. for pond, lake and river.
The present invention has the following advantages: the present invention is by processing load of microorganisms by fermentation in amaranth seed powder, and the amaranth seed powder forage made to wrap up in bag amaranth seed powder and microorganism can common purifying water body, and efficiently, lasting, with low cost, environmental safety is high.
Accompanying drawing explanation
Fig. 1 is the COD change of duration of test water inlet in embodiment 3;
Fig. 2 is the TN change of duration of test water inlet in embodiment 3;
Fig. 3 is the TP change of duration of test water inlet in embodiment 3;
Fig. 4 is the total plate count change of duration of test water inlet in embodiment 3;
Fig. 5 is that in embodiment 3, duration of test lake COD changes;
Fig. 6 is that in embodiment 3, duration of test lake TN changes;
Fig. 7 is total plate count change in duration of test lake in embodiment 3.
Embodiment
Below in conjunction with embodiment, the present invention is described in further details, but embodiments of the present invention are not limited thereto.
Embodiment 1
The amaranth seed powder forage adopted in the present invention is wrapped up in bag and is prepared by the following method: by the amaranth seed powder of harvesting after comprising pulverizing and predrying operation process, amaranth seed powder sprays complex microorganism preparations, make its amount reproduction and be gathered in forage, under anaerobic carry out fermentative processing, again after comprising bundling and wrapping up in the process of job contract sequence, finally make amaranth seed powder forage and wrap up in bag.
Be crushed to the length of amaranth seed powder for about 1.0cm, broken joint rate is more than 75%.
Predryingly be about 65% to the mass percentage of moisture in amaranth seed powder.
Complex microorganism preparations in the present invention, is made up of fermentation of bacillus liquid, nitrobacteria fermented liquid and Fermentation by Photosynthetic Bacteria liquid, and the consumption of this complex microorganism preparations is account for amaranth seed powder total mass 10%.This complex microorganism preparations has high reactivity and organics removal ability.
Wherein the mass percentage of fermentation of bacillus liquid, nitrobacteria fermented liquid and Fermentation by Photosynthetic Bacteria liquid three is respectively 40%, 25% and 35%.
Wherein genus bacillus, nitrobacteria and photosynthetic bacterium are commercially available bacterial classification, the preparation method of fermentation of bacillus liquid, nitrobacteria fermented liquid and Fermentation by Photosynthetic Bacteria liquid is: get genus bacillus, nitrobacteria and photosynthetic bacterium bacterial classification respectively, by its specification sheets or ordinary method activated, enlarged culturing process respectively, be prepared into concentration respectively and be about 1 × 10
7the genus bacillus kind liquid of individual/g, nitrobacteria kind liquid or photosynthetic bacterium kind liquid, by genus bacillus kind liquid, nitrobacteria kind liquid, photosynthetic bacterium kind liquid inoculation fermentation on respective substratum respectively, fermentation of bacillus liquid, nitrobacteria fermented liquid or Fermentation by Photosynthetic Bacteria liquid can be obtained.
Wherein genus bacillus kind liquid adopts broth agar culture medium.
Nitrobacteria kind liquid can adopt culture medium prescription 1 to be: peptone: 1%, yeast extract paste: 0.5%, sodium-chlor: 1%, agar: 2%, distilled water: 100mL, pH value: 7.2-7.5.
The conventional liquid culture based formulas that photosynthetic bacterium can adopt is: NH
4cl1.0g, CH
3cOONa3.5g, MgC1
20.1g, CaC1
20.1g, KH
2pO
40.6g, K
2hPO
40.4g, yeast extract paste 0.1g, water 1000mL, pH7.2.
Under anaerobic carry out, complex microorganism preparations is sprayed in amaranth seed powder, in 20 DEG C of fermentations process in 30 days, then after comprising bundling and wrapping up in the process of job contract sequence, finally make amaranth seed powder forage and wrap up in bag.
The amaranth seed powder forage made is wrapped up in after bag storage carries out continuation fermentative processing in 40 days and namely be can be used for purifying water body.
Embodiment 2
The amaranth seed powder forage adopted in the present invention is wrapped up in bag and is prepared by the following method: by the amaranth seed powder of harvesting after comprising pulverizing and predrying operation process, amaranth seed powder sprays complex microorganism preparations, make its amount reproduction and be gathered in forage, under anaerobic carry out fermentative processing, again after comprising bundling and wrapping up in the process of job contract sequence, finally make amaranth seed powder forage and wrap up in bag.
The length being crushed to amaranth seed powder is about 1.5cm, and broken joint rate is preferably more than 75%.
Predryingly be about 75% to the mass percentage of moisture in amaranth seed powder.
Complex microorganism preparations in the present invention, is made up of fermentation of bacillus liquid, nitrobacteria fermented liquid and Fermentation by Photosynthetic Bacteria liquid, and the consumption of this complex microorganism preparations is account for amaranth seed powder total mass 8%.This complex microorganism preparations has high reactivity and organics removal ability.
Wherein the mass percentage of fermentation of bacillus liquid, nitrobacteria fermented liquid and Fermentation by Photosynthetic Bacteria liquid three is respectively 30%, 30% and 40%.
Wherein genus bacillus, nitrobacteria and photosynthetic bacterium are commercially available bacterial classification, the preparation method of fermentation of bacillus liquid, nitrobacteria fermented liquid and Fermentation by Photosynthetic Bacteria liquid is: get genus bacillus, nitrobacteria and photosynthetic bacterium bacterial classification respectively, by its specification sheets or ordinary method activated, enlarged culturing process respectively, be prepared into concentration respectively and be about 1 × 10
8the genus bacillus kind liquid of individual/g, nitrobacteria kind liquid or photosynthetic bacterium kind liquid, by genus bacillus kind liquid, nitrobacteria kind liquid, photosynthetic bacterium kind liquid inoculation fermentation on respective substratum respectively, fermentation of bacillus liquid, nitrobacteria fermented liquid or Fermentation by Photosynthetic Bacteria liquid can be obtained.
Wherein genus bacillus kind liquid can adopt beef-protein medium.
Nitrobacteria kind liquid can adopt culture medium prescription 2 to be: potato: 20%, peptone: 0.5%, white sugar: 1%, glucose: 1%, mixed inorganic: 1%, agar: 2%, tap water: 100mL, pH:7.0.
Photosynthetic bacterium can adopt liquid nutrient medium, and liquid culture based formulas is: NH
4cl1.0g, CH
3cOONa3.5g, MgC1
20.1g, CaC1
20.1g, KH
2pO
40.6g, K
2hPO
40.4g, yeast extract paste 0.1g, water 1000mL, pH7.2.
Under anaerobic carry out, complex microorganism preparations is sprayed in amaranth seed powder, in 30 DEG C of fermentations process in 25 days, then after comprising bundling and wrapping up in the process of job contract sequence, finally make amaranth seed powder forage and wrap up in bag.
The amaranth seed powder forage made is wrapped up in after bag storage carries out continuation fermentative processing in 50 days and namely be can be used for purifying water body.
Embodiment 3
The amaranth seed powder forage adopted in the present invention is wrapped up in bag and is prepared by the following method: by the amaranth seed powder of harvesting after comprising pulverizing and predrying operation process, amaranth seed powder sprays complex microorganism preparations, make its amount reproduction and be gathered in forage, under anaerobic carry out fermentative processing, again after comprising bundling and wrapping up in the process of job contract sequence, finally make amaranth seed powder forage and wrap up in bag.
The length being crushed to amaranth seed powder is about 1.2cm, and broken joint rate is preferably more than 75%.
Predryingly be about 70% to the mass percentage of moisture in amaranth seed powder.
Complex microorganism preparations in the present invention, is made up of fermentation of bacillus liquid, nitrobacteria fermented liquid and Fermentation by Photosynthetic Bacteria liquid, and the consumption of this complex microorganism preparations is account for amaranth seed powder total mass 12%.This complex microorganism preparations has high reactivity and organics removal ability.
Wherein the mass percentage of fermentation of bacillus liquid, nitrobacteria fermented liquid and Fermentation by Photosynthetic Bacteria liquid three is respectively 40%, 30% and 30%.
This complex microorganism preparations has high reactivity and organics removal ability.Wherein genus bacillus, nitrobacteria and photosynthetic bacterium are commercially available bacterial classification.Also can purchased from Chinese industrial Microbiological Culture Collection administrative center etc.
The preparation method of fermentation of bacillus liquid, nitrobacteria fermented liquid and Fermentation by Photosynthetic Bacteria liquid is: get genus bacillus, nitrobacteria and photosynthetic bacterium bacterial classification respectively, by its specification sheets or ordinary method activated, enlarged culturing process respectively, being prepared into concentration is respectively 1.5 × 10
7the genus bacillus kind liquid of individual/g, nitrobacteria kind liquid or photosynthetic bacterium kind liquid, by genus bacillus kind liquid, nitrobacteria kind liquid, photosynthetic bacterium kind liquid inoculation fermentation on respective substratum respectively, fermentation of bacillus liquid, nitrobacteria fermented liquid or Fermentation by Photosynthetic Bacteria liquid can be obtained.
Wherein genus bacillus kind liquid can adopt potato dextrose agar etc.
Nitrobacteria kind liquid can adopt culture medium prescription 3 to be: peptone: 1%, extractum carnis: 0.3%, sodium-chlor: 0.5%, agar: 2%, distilled water: 100mL, pH value: 7.0 etc.
The conventional liquid culture based formulas that photosynthetic bacterium can adopt is: NH
4cl1.0g, CH
3cOONa3.5g, MgC1
20.1g, CaC1
20.1g, KH
2pO
40.6g, K
2hPO
40.4g, yeast extract paste 0.1g, water 1000mL, pH7.2.
Under anaerobic carry out, complex microorganism preparations is sprayed in amaranth seed powder, in 40 DEG C of fermentations process in 20 days, then after comprising bundling and wrapping up in the process of job contract sequence, finally make amaranth seed powder forage and wrap up in bag.
The amaranth seed powder forage made is wrapped up in after bag storage carries out continuation fermentative processing in 50 days and namely be can be used for purifying water body.
Embodiment 4
The amaranth seed powder forage of preparation in embodiment 1 is wrapped up in bag, be invested in the simulation water body in test site, throw in 1 every day, release time is 10:30-11:30 in the morning, set the blank (CK) and the contrast of throwing single culture water quality situation that do not add thalline, the changing conditions of the water-quality guideline such as serial sampling monitoring water body COD, TN, TP, total plate count and turbidity simultaneously.Measurement result is as shown in table 1 below:
The changing conditions that single culture and amaranth seed powder forage wrap up in bag water-quality guideline thrown in by table 1
As can be known from Table 1, microbial preparation is sprayed at the amaranth seed powder forage that amaranth seed powder is formed after anaerobically fermenting is cultivated and wraps up in bag, when being used alone than microbial preparation, the better effects if of purifying water body, and amaranth seed powder forage is wrapped up in bag and is all had obvious removal effect to water body COD, BOD, TN and turbidity etc., and significantly can promote Dissolved Oxygen in Water.In addition amaranth seed powder forage wraps up in bag, and processing is simple, and storage is convenient, and suitability is strong, can remove nitrogen, also can remove organic matter simultaneously, have broad application prospects.
Embodiment 5
The present embodiment is from the different areas hydrology and microbial diversity, with typical lake, somewhere, Guangdong one for research object, carry out amaranth seed powder forage and wrapped up in the research of bag (in embodiment 1 preparation amaranth seed powder forage wrap up in bag) to water body in lake purification and water remediation.
Wherein duration of test water inlet water body situation as shown in figs. 1-4.
This amaranth seed powder forage wraps up in bag to the removal effect of chemical oxygen demand COD as shown in Figure 5, as shown in Figure 5, when test is just carried out, the COD change in concentration amplitude of 4, test site point (water outlet A, sampling spot B, sampling spot C and water-in D) is larger, mainly because bed mud and water body deposit have been stirred in construction, the disturbance of bed mud can discharge a part of nutritive salt, and simultaneously surface water settling has the more plant and animal residues be not decomposed, and causes increasing of COD concentration in waters, local.
Throw in amaranth seed powder forage and wrap up in bag after 1 week, the COD concentration of sampling point B, C and water outlet starts to reduce gradually, test proceeds to November 14, the COD of water-in is 17.55mg/L, and water outlet is 11.21mg/L, and clearance is 4.5%, reach maximum value, form advantage gradually along with amaranth seed powder forage is wrapped up in wrap in test waters, the function of its specific organics removal shows, thus can effectively reduce COD concentration.
This amaranth seed powder forage wraps up in bag to the removal effect of total nitrogen as shown in Figure 6, as shown in Figure 6, first 1 week of on-test, not obvious to the removal effect of total nitrogen (TN), along with in test waters, amaranth seed powder forage wraps up in the propagation of bacterium in bag, the downtrending of TN is obvious, TN concentration generally about the 0.30mg/L lower than water-in of water outlet.Meanwhile, the clearance of TN is also substantially in rising trend.
The TP change in concentration amplitude of duration of test test water sample is little, is basically stable at start numbers, although illustrate that this amaranth seed powder forage is wrapped up in bag and had certain effect, DeGrain to total phosphorus.
This amaranth seed powder forage wraps up in bag on the impact of total plate count as shown in Figure 7, and as can be seen from Figure 7, during on-test, the total plate count in test waters is not high, and after input amaranth seed powder forage wraps up in bag, total plate count obviously raises.And along with the direction of current, roughly in the trend reduced gradually.This is because the amaranth seed powder forage microorganism wrapped up in bag has adapted to the water body environment of test site gradually, define growth vigor and amount reproduction.To later stage, water temperature reduces, and the reproduction speed of microorganisms in water reduces, thus causes total plate count to reduce.
Obviously, foregoing just in order to feature of the present invention is described, and is not limitation of the present invention, and the those of ordinary skill of relevant technical field should belong to protection category of the present invention according to the present invention in the change that corresponding technical field is made.
Claims (9)
1. amaranth seed powder forage wraps up in the application wrapped in purifying water body.
2. amaranth seed powder forage according to claim 1 wraps up in the application wrapped in purifying water body, it is characterized in that: described amaranth seed powder forage is wrapped up in bag and prepared by the following method: by amaranth seed powder after comprising pulverizing and predrying operation process, amaranth seed powder sprays complex microorganism preparations, make its amount reproduction and be gathered in forage, under anaerobic carry out fermentative processing, again after comprising bundling and wrapping up in the process of job contract sequence, finally make amaranth seed powder forage and wrap up in bag.
3. amaranth seed powder forage according to claim 2 wraps up in the application wrapped in purifying water body, it is characterized in that: described complex microorganism preparations is made up of fermentation of bacillus liquid, nitrobacteria fermented liquid and Fermentation by Photosynthetic Bacteria liquid, the consumption of described complex microorganism preparations accounts for 8 ~ 12% of amaranth seed powder total mass.
4. amaranth seed powder forage according to claim 3 wraps up in the application wrapped in purifying water body, it is characterized in that: the mass percentage of described fermentation of bacillus liquid, nitrobacteria fermented liquid and Fermentation by Photosynthetic Bacteria liquid is respectively 30 ~ 40%, 20 ~ 30% and 30 ~ 50%.
5. amaranth seed powder forage according to claim 2 wraps up in the application wrapped in purifying water body, it is characterized in that: under anaerobic regulate temperature to be 20 ~ 40 DEG C and carry out fermentative processing 20 ~ 30 days, again after comprising bundling and wrapping up in the process of job contract sequence, finally make amaranth seed powder forage and wrap up in bag.
6. amaranth seed powder forage according to claim 2 wraps up in the application wrapped in purifying water body, it is characterized in that: the amaranth seed powder forage made is wrapped up in after bag storage carries out continuation fermentative processing in 40 ~ 60 days and namely be can be used for purifying water body.
7. amaranth seed powder forage according to claim 2 wraps up in the application wrapped in purifying water body, it is characterized in that: the length being crushed to amaranth seed powder is 1.0 ~ 1.5cm, and broken joint rate is more than 75%.
8. amaranth seed powder forage according to claim 2 wraps up in the application wrapped in purifying water body, it is characterized in that: the predrying mass percentage to moisture in amaranth seed powder is 65 ~ 75%.
9. amaranth seed powder forage according to claim 2 wraps up in the application wrapped in purifying water body, it is characterized in that: after bundling, adopts and twines film and wrap up in bag, and twine film when wrapping up in bag, twining rete number is 4 ~ 6 layers.
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| CN201510645260.9A CN105254026A (en) | 2015-09-30 | 2015-09-30 | Application of amaranth forage parcel in water purification |
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| CN101157892A (en) * | 2007-03-06 | 2008-04-09 | 扬州市绿科生物技术有限公司 | Production method of solid preparation for degradation of ammonia nitrogen and nitrite |
| CN101269884A (en) * | 2008-05-08 | 2008-09-24 | 储卫华 | Microorganism renovation agent of water environment and preparation method thereof |
| CN102839125A (en) * | 2012-09-19 | 2012-12-26 | 天津市农业生物技术研究中心 | Solid fermentation method of straw fermentation bio-additive and application |
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Application publication date: 20160120 |
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| RJ01 | Rejection of invention patent application after publication |