CN102839125A - Solid fermentation method of straw fermentation bio-additive and application - Google Patents
Solid fermentation method of straw fermentation bio-additive and application Download PDFInfo
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- CN102839125A CN102839125A CN2012103486224A CN201210348622A CN102839125A CN 102839125 A CN102839125 A CN 102839125A CN 2012103486224 A CN2012103486224 A CN 2012103486224A CN 201210348622 A CN201210348622 A CN 201210348622A CN 102839125 A CN102839125 A CN 102839125A
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Abstract
The invention discloses a solid fermentation method of a straw fermentation bio-additive. The method is characterized by comprising the following steps of: mixing solid media according to the ratio of water to bean pulp powder to wheat bran powder to cane sugar to urea being 400:200:200:10:5; split charging into wild-mouth bottles after uniformly mixing; sterilizing for 20 minutes at the temperature of 121 DEG C; inoculating a straw fermentation bio-additive liquid strain into each bottle according to the proportion of 10-20 percent; and carrying out static cultivation for 48-72h at the temperature of 35 DEG C, wherein after natural airdrying, the microbial content in fungicide reaches 700*108/g. According to the method, the production cost can be reduced, the product quality and the effectiveness are improved; and when the method is applied to water body purification, the nitrite in a water body can be degraded, a pH value of the water body is reduced, the water body is purified, and wide application prospect is realized.
Description
Technical field
Grind in the art field that the present invention relates to environmental microorganism and microbial fermentation skill, say so more specifically a kind of biogenic additives for fermentation of stalks solid fermentation method and the application on water body purification thereof.
Background technology
This patent is the production technology of on the basis of patent " biogenic additives for fermentation of stalks and collocation method thereof " (patent No. ZL97119176.X), carrying out and the improvement research of range of application." biogenic additives for fermentation of stalks " made through the biotechnology fermentation by the homogeneity milk-acid bacteria of streptococcus faecium, pediococcus cerevisiae, plant lactobacillus, acid anhydride buttermilk bacillus, Dai Shi probiotic lactobacillus and the Bacillus licheniformis 6 strain L type lactic acid ratio science compatibility in 1:1:1:1:1:1.Can make wheat straw, straw and the yellow corn stalk of doing in ten days, become the fragrant agreeable to the taste cattle and sheep feed of a kind of acid, its business is worth, palatability greatly improves than raw material.To promoting growth of animal, improve the animal metabolism function, aspects such as increase animal disease resistant ability have a very important role.This patent has good economic benefits and social benefit.But in practical application; There are the manpower and materials of labor in this microbial inoculum in batch process, production stability is relatively poor, the more high shortcoming of production cost; Influenced the industrialization process of this patent; And solid fermentation can be directly be solid form with the formulation fermentation, has reduced absorption again behind the liquid fermenting, the exsiccant process.And the bacterium number of solid fermentation is higher than liquid fermenting bacterium number far away, and the quality of product and validity are improved greatly, and the ferment effect of solid fermentation agent and liquid starter is similar in the fermented stalk process.In addition, the inventor finds that further biogenic additives for fermentation of stalks is applied to purification of waste water and has effect preferably, and consideration is used for this microbial inoculum the mikrobe repair process of breeding wastewater or sewage.Obviously improve the production efficiency of biogenic additives for fermentation of stalks behind completion the present invention, enlarged its use range.
Summary of the invention
An object of the present invention is to provide solid fermentation method simple and easy, the efficient straw biogenic additives for fermentation.
Another object of the present invention has provided the application of a kind of biogenic additives for fermentation of stalks aspect water body purification.
For realizing above-mentioned purpose, the invention discloses following technology contents:
A kind of solid fermentation method of biogenic additives for fermentation of stalks is characterized in that being undertaken by following step:
(1) with being sub-packed in the wide-necked bottle behind the solid medium mixing, in 121 ℃ of high-temperature sterilization 15-20min, the ratio of weight and number of described solid medium is a water: bean cake powder: wheat bran powder: sucrose: urea is respectively with 400:200:200: 10: 5;
(2) meet the liquid spawn that the bacterium amount is inoculated mixed culture, static fermentation culture 48-72 h in 35 ℃ of constant incubators according to 10-20%;
(3) behind 20-35 ℃ of natural air drying 5 d in fermentation back, the bacterium number reaches 700 * 10
8Individual/g; Wherein said liquid spawn refers to: biogenic additives for fermentation of stalks.
The more detailed solid fermentation method of the present invention is: with water: bean cake powder: wheat bran powder: sucrose: urea is respectively with 400:200:200: mix at 10: 5; Be sub-packed in the wide-necked bottle behind the mixing; Every bottle of 200g; 121 ℃ of sterilization 20min, every bottle graft 20mL liquid spawn places 35 ℃ of static cultivation 48 h.The fermentation back is measured total count and is reached 44 * 108/g, and behind 20-35 ℃ of natural air drying 5 d, the bacterium number reaches 700 * 108/g.
Liquid spawn provided by the invention (patent No. ZL 97119176.X; Introducing the present invention) " biogenic additives for fermentation of stalks " in carries out the 48-72h single culture respectively by the homogeneity milk-acid bacteria of streptococcus faecium, pediococcus cerevisiae, plant lactobacillus, acid anhydride buttermilk bacillus, Dai Shi probiotic lactobacillus and Bacillus licheniformis 6 strain L type lactic acid in culture medium solution; Culture temperature is 20-30 ℃, add 6 kinds of bacterial classifications volume be 1% of culture medium solution volume; Culture medium solution consist of yeast extract paste 4-6g, glucose 8-15g, peptone 5-10g; Potassium primary phosphate 1-3g, water 1000ml, pH 6.0-7.0; The equivalent single culture liquid of drawing above-mentioned 6 kinds of bacterial classifications joins and carries out mixed culture in the same culture medium solution respectively again, add 6 kinds of bacterial classification single culture liquid the volume total amount be the 5-15% of present culture medium solution volume, culture temperature is 20-30 ℃; Time is 48-72h, uses microscopy, and the bacterium number reaches 10 in the nutrient solution
8Individual/ml, be qualified liquid spawn.
The present invention further discloses biogenic additives for fermentation of stalks is using aspect the preparation water body purification.Its water body purification refers to: reduce the nitrite in the water body fast, reduce the pH value of water body.Mainly be the nitrite in the degraded water body in aquaculture wastewater or waste water, reduce the pH value of water body, its main advantage is:
(1) the biogenic additives for fermentation of stalks solid fermentation method is simple and easy to do, and is with low cost, increased thalline quantity simultaneously, improved the quality and the validity of product.
(2) the biogenic additives for fermentation of stalks water body Central Asia nitrate salt of can degrading rapidly and thoroughly reduces water pH value.
(3) because biogenic additives for fermentation of stalks is the compound micro-ecological preparation that feed adds class, nontoxic to aquaculture organism, can handle at environmental wastewater, use in a plurality of fields such as aquaculture pond.
Description of drawings:
Fig. 1 is the degradation effect of biogenic additives for fermentation of stalks to nitrite nitrogen in the analog culture waste water;
Fig. 2 is the influence of biogenic additives for fermentation of stalks to pH value in the analog culture waste water.
Embodiment
Below in conjunction with embodiment the present invention is described; The scheme of embodiment described here; Do not limit the present invention; One of skill in the art can make improvements and change according to spirit of the present invention, and described these improvement and variation all should be regarded as within the scope of the invention, and scope of the present invention and essence are limited claim.Wherein streptococcus faecium (
Streptococcus faecium) (Chinese microorganism strain preservation center: ACCC 10180), pediococcus cerevisiae (
Pediococcus cerecisiae) (Chinese industrial microbial strains preservation administrative center: CICC 22727), plant lactobacillus (
Lactobacillus plantarum) (Chinese microorganism strain preservation center: ACCC 11016), acid anhydride buttermilk bacillus (
Lactobacillus casei) (Chinese food fermentation industry Science Institute: bacterial strain deposit number 10640 scenes obtain), Dai Shi probiotic lactobacillus (
Lactobacillus delbrueckii) (Chinese common micro-organisms preservation administrative center CGMCC 1.2131) and Bacillus licheniformis (
Bacillus licheniformis) (Chinese microorganism strain preservation center: CICIM B0001) above-mentioned bacterial classification all has commercially available.
Embodiment 1
The preparation of inoculation liquid spawn:
(1) streptococcus faecium, pediococcus cerevisiae, plant lactobacillus, acid anhydride buttermilk bacillus, Dai Shi probiotic lactobacillus and each 1ml of Bacillus licheniformis that gets equivalent earlier is seeded in 100ml respectively in the nutrient solution of high-temperature sterilization (20min, 121 ℃), cultivates 48h for 30 ℃.The prescription of nutrient solution is yeast extract paste 4-6g, glucose 8-15g, peptone 5-10g, potassium primary phosphate 1-3g, water 1000ml, pH 6.0-7.0.
(2) the bacterium liquid of getting six strain single culture is 600ml altogether, joins among nutrient solution (it is the same fill a prescription) 11.4 L of same high-temperature sterilization (20min, 121 ℃) to carry out mixed culture, 30 ℃ of cultivation 48h.Use microscopy, number of bacteria reaches 10 in the nutrient solution
8Individual/ml is qualified, obtains mixed bacteria liquid 12L, is required liquid spawn.
Embodiment 2
The bacterium number of " biogenic additives for fermentation of stalks " liquid fermenting and solid fermentation relatively
(1) liquid fermenting
Configuration nutrient solution (it is the same to fill a prescription) 3L, the 5 L fermentor tanks of packing into, 121 ℃ of sterilization 20min; Insert the liquid spawn 300ml of mixed culture; 30 ℃ of temperature, pH 5.0, stirring velocity 300 r/min; Blended bacterium liquid reaches fermentation termination at 24 h under the optimal conditions of fermentation of air flow 4 L/min, and the maximum bacterial concentration of fermentation termination can reach 27.5 * 10
8Individual/more than the ml.
(2) solid fermentation
With water: bean cake powder: wheat bran powder: sucrose: urea is respectively with 400:200:200: mix at 10: 5, and packing behind the mixing, every bottle of 200-400g, 121 ℃ of high-temperature sterilization 15min, every bottle graft 20mL liquid spawn places 35 ℃ of static cultivation 48 h.Hygrometric bacteria concentration behind 2 d.With surveying dried bacteria concentration behind the microbial inoculum natural air drying 5d.
(3) behind 20-35 ℃ of natural air drying 5 d in fermentation back, the bacterium number reaches 700 * 10
8Individual/g; Wherein said liquid spawn refers to: biogenic additives for fermentation of stalks.
The result shows: the product behind solid fermentation method fermentation and the liquid fermenting is compared, and strand a special aroma and a beany flavour are arranged after the fermentation.Maximum bacterial concentration can reach 27.5 * 10 after the liquid fermenting secondary fermentation
8Individual/more than the ml, behind the solid fermentation in the wet bacterium of every gram total count reach 44 * 10
8Individual/g (table 1), exceed more than 1 times than the total count of liquid bacterial agent.The bacteria containing amount of the dried bacterium of the air-dry every gram in back reaches 700 * 10
8Individual/g, than liquid fermentation agent Senior Two more than ten times.
The cell concentration of table 1 liquid, solid fermentation relatively
| Microbial inoculum | Liquid fermenting | Wet bacterium (before dry) | Dried bacterium (dry back) |
| Bacterium number (10 8Individual/g) | 27.5 | 44 | 710 |
(3) the active maintenance situation of the thalline of solid fermentation microbial inoculum
After microbial inoculum room temperature behind the solid fermentation is air-dry, pack, room temperature preservation, 6 months, resampling, simultaneously with blood counting chamber be coated with two kinds of methods of flat band method survey bacterium number.The result is like (table 2).Use blood counting chamber to find that the total count of the dried bacterium of every gram is 340 * 10
8Individual/g, and show that with the result of colony counting method bacteria containing amount reaches 573 * 10 in the dried bacterium of every gram
8Individual/g, the microbial inoculum that solid fermentation is described after 6 months the thalline activity remain on more than 80%, the activity that solid fermentation can the better preserve thalline is described, be convenient to the storage of thalline.
The cell concentration of table 2 solid fermentation over time
Embodiment 3
(1) with being sub-packed in the wide-necked bottle behind the solid medium mixing, in 121 ℃ of high-temperature sterilization 15min, the ratio of weight and number of described solid medium is a water: bean cake powder: wheat bran powder: sucrose: urea is respectively with 400:200:200: 10: 5;
(2) meet the liquid spawn that the bacterium amount is inoculated mixed culture, static fermentation culture 72 h in 35 ℃ of constant incubators according to 20%;
(3) behind back 30 ℃ of natural air drying 5 d of fermentation, the bacterium number reaches 700 * 10
8Individual/g; Wherein said liquid spawn refers to: biogenic additives for fermentation of stalks.
Embodiment 4
The effect of liquid bacterial agent and solid fungicide fermented feed relatively
Prepare 3 in identical stalk fermentation pond, about each 3 tons.Stalk should cut up with a hay cutter in advance and cut, and hand hay cutter is cut length less than 30 mm.About 9 tons altogether.Microbial inoculum is not inoculated for contrast in No. 1 pond, directly puts into stalk, adds an amount of 0.9% salt solution, makes water ratio reach 60%～70%, uses the plastic cloth compacting, air-isolation, spontaneous fermentation.No. 2 the pond is the inoculation liquid bacterial agent; Stalk per ton adds 50 ml biogenic additives for fermentation of stalks bacterium liquid, according to 1:50 microbial inoculum is diluted with warm boiling water, and the liquid bacterial agent for preparing is sprayed on the stalk with power sprayer or manual work; And overturning 2～3 times, bacterium liquid is evenly distributed.Add an amount of 0.9% salt solution, make water ratio reach 60%～70%, use the plastic cloth compacting, air-isolation, fermentation.No. 3 ponds are the inoculation solid fungicide, and stalk per ton adds 20 g biogenic additives for fermentation of stalks, mix an amount of Semen Maydis powder or wheat bran evenly is sprinkling upon on stalk according to 1:50, and overturning 2～3 times, and bacterium is evenly distributed.Add an amount of 0.9% salt solution, make water ratio reach 60%～70%, use the plastic cloth compacting, air-isolation, fermentation.
Measure after 10-15 days and find, have in the stalk in No. 1 contrast pond mildew and rot, rotten, the heating phenomenon, smell is relatively poor, suitable feeding.And connect in 2, No. 3 ponds behind the stalk fermentation of bacterium biogenic additives for fermentation of stalks quality soft, moistening bulk, be wine aroma, palatability is fabulous, can promote cattle and sheep appetite, promoting digestion absorbs.
Utilize the stalk fermentation product feeding cow in the pond 2, No. 3, can make milk production of cow improve about 10%.The beef cattle of feeding finds that beef cattle growth is fast, delivers for sale early, grows smoothly, seldom uses veterinary drug, no chemical pollution, thereby commodity is good, is prone to sell.
Embodiment 5
" biogenic additives for fermentation of stalks " decontamination effect improving in analog culture waste water
With adorning 1 L analog culture waste water in the Glass Containers; The starting point concentration that nitrite nitrogen is measured in adjustment is 45mg/L, and liquid treatment inserts 5ml " biogenic additives for fermentation of stalks " bacterium liquid, and solids treatment inserts " biogenic additives for fermentation of stalks " microbial inoculum of 1g solid fermentation; Not add bacterium is contrast; Leave standstill cultivation in placing 30 ℃, every day, timing sampling was surveyed the content of nitrite nitrogen in the water, pH value.The result is like figure.
Can be found out by Fig. 1, compare with contrast that it is that the degradation rate of 45 mg/L simulated wastewaters is 79.11% that liquid bacterial agent is handled in 24 h the nitrite nitrogen concentration, be 89.8% with the nitrite nitrogen degradation rate within 48 h, and degradation effect is better; It is that the degradation rate of 45 mg/L simulated wastewaters is 99.7% that solid fungicide is handled in 24 h the nitrite nitrogen concentration, degraded basically fully.
Can find out by Fig. 2; In water body, insert " biogenic additives for fermentation of stalks "; The pH value that liquid bacterial agent is handled water body in the 72h all is lower than contrast 0.1-0.3 unit, and the pH value that solid fungicide is handled water body in the 72h all is lower than contrast 0.4-1.3 unit, and solids treatment pH value is lower than liquid treatment pH value; Compare with contrast, connect bacterium and handle the pH value that has significantly reduced water body.
Claims (3)
1. the solid fermentation method of a biogenic additives for fermentation of stalks is characterized in that being undertaken by following step:
(1) with being sub-packed in the wide-necked bottle behind the solid medium mixing, in 121 ℃ of high-temperature sterilization 15-20min, the ratio of weight and number of described solid medium is a water: bean cake powder: wheat bran powder: sucrose: urea is respectively with 400:200:200: 10: 5;
(2) meet the liquid spawn that the bacterium amount is inoculated mixed culture, static fermentation culture 48-72 h in 35 ℃ of constant incubators according to 10-20%;
(3) behind 20-35 ℃ of natural air drying 5 d in fermentation back, the bacterium number reaches 700 * 10
8Individual/g; Wherein said liquid spawn refers to: biogenic additives for fermentation of stalks.
2. the said biogenic additives for fermentation of stalks solid fermentation method of claim 1 is used aspect water body purification in preparation.
3. the described application of claim 2, wherein water body purification refers to: reduce the nitrite in the water body fast, reduce the pH value of water body.
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105254026A (en) * | 2015-09-30 | 2016-01-20 | 胡小勇 | Application of amaranth forage parcel in water purification |
| CN105567585A (en) * | 2015-12-16 | 2016-05-11 | 天津市畜牧兽医研究所 | Compound microbial agent for silage and application thereof in whole-plant corn silage |
| CN106929441A (en) * | 2015-12-30 | 2017-07-07 | 湖北华扬科技发展有限公司 | A kind of cultural method of enterococcus faecalis |
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| CN1178077A (en) * | 1997-10-24 | 1998-04-08 | 天津市农业生物工程研究中心 | Biogenic additives for fermentation of stalks and preparation thereof |
| CN1706270A (en) * | 2004-06-05 | 2005-12-14 | 宁夏大学 | Stalks fermenting fungus prepn and production process and biologically fermented stalks feed |
| CN101659951A (en) * | 2008-08-29 | 2010-03-03 | 天津市农业生物技术研究中心 | Immobilized cultivation method for 'microbiological straw additive' |
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- 2012-09-19 CN CN201210348622.4A patent/CN102839125B/en not_active Expired - Fee Related
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN1178077A (en) * | 1997-10-24 | 1998-04-08 | 天津市农业生物工程研究中心 | Biogenic additives for fermentation of stalks and preparation thereof |
| CN1706270A (en) * | 2004-06-05 | 2005-12-14 | 宁夏大学 | Stalks fermenting fungus prepn and production process and biologically fermented stalks feed |
| CN101659951A (en) * | 2008-08-29 | 2010-03-03 | 天津市农业生物技术研究中心 | Immobilized cultivation method for 'microbiological straw additive' |
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105254026A (en) * | 2015-09-30 | 2016-01-20 | 胡小勇 | Application of amaranth forage parcel in water purification |
| CN105567585A (en) * | 2015-12-16 | 2016-05-11 | 天津市畜牧兽医研究所 | Compound microbial agent for silage and application thereof in whole-plant corn silage |
| CN106929441A (en) * | 2015-12-30 | 2017-07-07 | 湖北华扬科技发展有限公司 | A kind of cultural method of enterococcus faecalis |
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