CN101659951A - Immobilized cultivation method for 'microbiological straw additive' - Google Patents
Immobilized cultivation method for 'microbiological straw additive' Download PDFInfo
- Publication number
- CN101659951A CN101659951A CN200810054346A CN200810054346A CN101659951A CN 101659951 A CN101659951 A CN 101659951A CN 200810054346 A CN200810054346 A CN 200810054346A CN 200810054346 A CN200810054346 A CN 200810054346A CN 101659951 A CN101659951 A CN 101659951A
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- CN
- China
- Prior art keywords
- microbiological
- immobilized
- straw
- additive
- straw additive
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- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
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- 239000010902 straw Substances 0.000 title claims abstract description 28
- 239000000654 additive Substances 0.000 title claims abstract description 27
- 230000000996 additive effect Effects 0.000 title claims abstract description 27
- 230000002906 microbiologic effect Effects 0.000 title claims abstract description 26
- 238000012364 cultivation method Methods 0.000 title claims abstract description 11
- 239000003795 chemical substances by application Substances 0.000 claims abstract description 10
- 239000001963 growth medium Substances 0.000 claims abstract description 8
- 241000894006 Bacteria Species 0.000 claims abstract description 7
- 230000001580 bacterial effect Effects 0.000 claims abstract description 7
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Chemical compound O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims abstract description 7
- 239000000203 mixture Substances 0.000 claims abstract description 6
- IXPNQXFRVYWDDI-UHFFFAOYSA-N 1-methyl-2,4-dioxo-1,3-diazinane-5-carboximidamide Chemical compound CN1CC(C(N)=N)C(=O)NC1=O IXPNQXFRVYWDDI-UHFFFAOYSA-N 0.000 claims abstract description 4
- 239000004372 Polyvinyl alcohol Substances 0.000 claims abstract description 4
- 229920002451 polyvinyl alcohol Polymers 0.000 claims abstract description 4
- 235000010413 sodium alginate Nutrition 0.000 claims abstract description 4
- 239000000661 sodium alginate Substances 0.000 claims abstract description 4
- 229940005550 sodium alginate Drugs 0.000 claims abstract description 4
- 238000000855 fermentation Methods 0.000 claims description 15
- 230000004151 fermentation Effects 0.000 claims description 15
- FRXSZNDVFUDTIR-UHFFFAOYSA-N 6-methoxy-1,2,3,4-tetrahydroquinoline Chemical compound N1CCCC2=CC(OC)=CC=C21 FRXSZNDVFUDTIR-UHFFFAOYSA-N 0.000 claims description 8
- 239000002068 microbial inoculum Substances 0.000 claims description 7
- WQZGKKKJIJFFOK-GASJEMHNSA-N Glucose Natural products OC[C@H]1OC(O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-GASJEMHNSA-N 0.000 claims description 3
- 229910019142 PO4 Inorganic materials 0.000 claims description 3
- 239000001888 Peptone Substances 0.000 claims description 3
- 108010080698 Peptones Proteins 0.000 claims description 3
- ZLMJMSJWJFRBEC-UHFFFAOYSA-N Potassium Chemical compound [K] ZLMJMSJWJFRBEC-UHFFFAOYSA-N 0.000 claims description 3
- 229940041514 candida albicans extract Drugs 0.000 claims description 3
- 239000008103 glucose Substances 0.000 claims description 3
- 239000002609 medium Substances 0.000 claims description 3
- 235000019319 peptone Nutrition 0.000 claims description 3
- NBIIXXVUZAFLBC-UHFFFAOYSA-K phosphate Chemical compound [O-]P([O-])([O-])=O NBIIXXVUZAFLBC-UHFFFAOYSA-K 0.000 claims description 3
- 239000010452 phosphate Substances 0.000 claims description 3
- 229910052700 potassium Inorganic materials 0.000 claims description 3
- 239000011591 potassium Substances 0.000 claims description 3
- 230000001105 regulatory effect Effects 0.000 claims description 3
- 230000003068 static effect Effects 0.000 claims description 3
- 230000001954 sterilising effect Effects 0.000 claims description 3
- 238000004659 sterilization and disinfection Methods 0.000 claims description 3
- 235000015193 tomato juice Nutrition 0.000 claims description 3
- 238000005406 washing Methods 0.000 claims description 3
- 239000012138 yeast extract Substances 0.000 claims description 3
- 238000004519 manufacturing process Methods 0.000 abstract description 12
- 238000000034 method Methods 0.000 abstract description 9
- 230000000694 effects Effects 0.000 abstract description 5
- 230000008901 benefit Effects 0.000 abstract description 4
- 230000008929 regeneration Effects 0.000 abstract description 2
- 238000011069 regeneration method Methods 0.000 abstract description 2
- 239000008187 granular material Substances 0.000 abstract 3
- UXVMQQNJUSDDNG-UHFFFAOYSA-L Calcium chloride Chemical compound [Cl-].[Cl-].[Ca+2] UXVMQQNJUSDDNG-UHFFFAOYSA-L 0.000 abstract 2
- 239000001110 calcium chloride Substances 0.000 abstract 2
- 229910001628 calcium chloride Inorganic materials 0.000 abstract 2
- 235000011148 calcium chloride Nutrition 0.000 abstract 2
- 238000011010 flushing procedure Methods 0.000 abstract 1
- 238000002156 mixing Methods 0.000 abstract 1
- 230000035755 proliferation Effects 0.000 abstract 1
- 238000002791 soaking Methods 0.000 abstract 1
- 239000008223 sterile water Substances 0.000 abstract 1
- 238000005516 engineering process Methods 0.000 description 5
- 230000000052 comparative effect Effects 0.000 description 2
- 230000000813 microbial effect Effects 0.000 description 2
- 244000005700 microbiome Species 0.000 description 2
- 241001465754 Metazoa Species 0.000 description 1
- 241000282849 Ruminantia Species 0.000 description 1
- 239000002253 acid Substances 0.000 description 1
- 238000010923 batch production Methods 0.000 description 1
- 230000007812 deficiency Effects 0.000 description 1
- 235000013325 dietary fiber Nutrition 0.000 description 1
- 239000000463 material Substances 0.000 description 1
- 235000019629 palatability Nutrition 0.000 description 1
- 239000002304 perfume Substances 0.000 description 1
- 238000002360 preparation method Methods 0.000 description 1
- 238000011084 recovery Methods 0.000 description 1
- 235000013599 spices Nutrition 0.000 description 1
Landscapes
- Immobilizing And Processing Of Enzymes And Microorganisms (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
Abstract
The invention discloses an immobilized cultivation method for 'microbiological straw additive', which comprises the following steps: mixing an activated 'microbiological straw additive' bacterial agent with an immobilized embedded carrier (mixture of 8 percent polyvinyl alcohol and 2 percent sodium alginate in a volume ratio of 0.6 to 1.4) in a volume ratio of 1 to 1, then dropping an embedding agent mixed with bacteria into the sterilized 0.2M CaCl2 solution in a super clean bench by using a medical injector of 10 milliliters and a matched needle, controlling the dropping speed, forming granules to embed the mixed bacterial agent therein, soaking the granules in the CaCl2 solution, flushing the granules by sterile water, and adding a proliferation culture medium into the solution to carryout timing cultivation. The method can fully exert the activity of strains, improve the tolerance of the strains, improve the operation stability of the bacterial agent production, simplify the operation process, simplify the reclamation problem of microbe at the later stage of the reaction, reduce the production cost and labor intensity, fully exert the advantages of microbe reaction, realize on-line regeneration of the microbe, and ensure the feasibility of the process.
Description
Technical field
The invention belongs to the method for microbial fermentation in the biotechnology, the immobilized cultivation method of particularly a kind of " microbiological straw additive ".
Background technology
This patent is (hereinafter to be referred as the improvement research of the production technology of carrying out on basis microbiological straw additive) in patent " Additive And Preparation Method Of Straw Fermenting Organism " (patent No. ZL97119176)." microbiological straw additive " can make half yellow or dried yellow all kinds of agricultural crop straws become the ruminant domestic animal roughage of smell acid perfume (or spice), moistening softness, good palatability in 7-10 days.This patent has good economic benefits and social benefit.But in the batch process of this microbial inoculum, exist and to expend a large amount of human and material resources production of hybrid seeds and production stability is relatively poor, the more high shortcoming of production cost, influenced the industrialization process of this patent, and the immobilization technology of widespread use has remedied these deficiencies just.
Summary of the invention
Technical problem to be solved by this invention is: the immobilized cultivation method that a kind of " microbiological straw additive " is provided.This technological method provides the immobilization culture technique that is fit to " microbiological straw additive " fermentation.
Technical scheme of the present invention is:
The immobilized cultivation method of a kind of " microbiological straw additive " is characterized in that:
Respectively with enduring of hot water, 0.6: 1.4 by volume mixed is with Ca (OH) with the polyvinyl alcohol of immobilized embedded carrier 8% and 2% sodium alginate
2It is 7.2 that solution is regulated its pH value, and the sterilization postcooling is with (wherein bacterial concentration is 10 behind activatory " microbiological straw additive " the microbial inoculum multiplication culture 18h
9Cfu/ml) mixed fermenting agent 1: 1 by volume mixes, the embedding medium that in super clean bench, will be mixed with thalline with 10ml injector for medical purpose and its supporting syringe needle splash into the bacterium of going out (15 pounds, 0.2MCaCl 30min)
2In the solution, speed is dripped in control, makes particulate state (the glueballs diameter is 2-3mm), mix bacterium agent is embedded in wherein, at CaCl
2Soak 30min in the solution, behind aseptic water washing 2-3 time, add proliferated culture medium and carry out the timing cultivation.
Its culture medium prescription is: every liter contains following composition: yeast extract paste 7.5g, and glucose 10g, peptone 7.5g, potassium primary phosphate 2g, the 100ml of Tomato juice, its pH value is 6.5-7.0.
" microbiological straw additive " thalline immobilization fermentation condition is: the suitableeest culture temperature is 30 ℃; Optimum pH is 6.0 static fermentation culture.This condition can make the stability of " microbiological straw additive " immobilization fermentation reach more than 10 batches.
Effect of the present invention is:
The immobilized cultivation method of this a kind of " microbiological straw additive " can reach the activity of giving full play to bacterial strain, improve its tolerance, improve the operational stability of microbial inoculum production and the technology that simplifies the operation, simplify the recovery problem of reaction later stage microorganism, reduce its production cost and labour intensity, give full play to the advantage of microbial reaction, realize the online regeneration of microorganism, thereby can guarantee the feasibility of technology.
Find by the comparative studies to free and two kinds of fermentation culture of immobilization of " microbiological straw additive " thalline: the immobilization fermentation of microbial inoculum can reach the free identical effect of fermenting, but make production cost reduce by 7%, the stability of production has improved 1.5 times.
Embodiment
The immobilized cultivation method of this a kind of " microbiological straw additive " provides the immobilization culture technique that is fit to " microbiological straw additive " fermentation.
Technological process is: activatory " microbiological straw additive " microbial inoculum is added in the proliferated culture medium, and (every liter of prescription of proliferated culture medium is: yeast extract paste 7.5g, glucose 10g, peptone 7.5g, potassium primary phosphate 2g, the 100ml of Tomato juice, the pH value is 6.5-7.0)
Respectively with enduring of hot water, 0.6: 1.4 by volume mixed is with Ca (OH) with the polyvinyl alcohol of immobilized embedded carrier 8% and 2% sodium alginate
2It is 7.2 that solution is regulated its pH value, and (bacterial concentration is 10 for sterilization postcooling and multiplication culture 18h
9Cfu/ml) mixed fermenting agent 1: 1 by volume mixes, the embedding medium that in super clean bench, will be mixed with thalline with 10ml injector for medical purpose and its supporting syringe needle splash into the bacterium of going out (15 pounds, 0.2M CaCl 30min)
2In the solution, speed is dripped in control, makes particulate state (the glueballs diameter is 2-3mm), mix bacterium agent is embedded in wherein, at CaCl
2Soak 30min in the solution, behind aseptic water washing 2-3 time, add proliferated culture medium and carry out the timing cultivation.
Find by the comparative studies to free and two kinds of fermentation culture of immobilization of " microbiological straw additive " thalline: the immobilization fermentation of microbial inoculum can reach the free identical effect of fermenting, but make production cost reduce by 7%, the stability of production has improved 1.5 times." microbiological straw additive " thalline immobilization fermentation condition is: 30 ℃ are the suitableeest culture temperature; 6.0 be the static fermentation culture of optimum pH.This condition can make the stability of " microbiological straw additive " immobilization fermentation reach more than 10 batches.
Claims (3)
1, the immobilized cultivation method of a kind of " microbiological straw additive " is characterized in that:
Respectively with enduring of hot water, 0.6: 1.4 by volume mixed is with Ca (OH) with the polyvinyl alcohol of immobilized embedded carrier 8% and 2% sodium alginate
2It is 7.2 that solution is regulated its pH value, the sterilization postcooling, with behind activatory " microbiological straw additive " the microbial inoculum multiplication culture 18h-wherein bacterial concentration is 10
9The mixed fermenting agent of cfu/ml-1: 1 by volume mixes, and the embedding medium that will be mixed with thalline with 10ml injector for medical purpose and its supporting syringe needle in super clean bench splashes into the bacterium of going out-15 pound, the 0.2MCaCl of 30min-
2In the solution, speed is dripped in control, and making particulate state-glueballs diameter is 2-3mm-, mix bacterium agent is embedded in wherein, at CaCl
2Soak 30min in the solution, behind aseptic water washing 2-3 time, add proliferated culture medium and carry out the timing cultivation.
2, the immobilized cultivation method of " microbiological straw additive " according to claim 1, it is characterized in that: its culture medium prescription is every liter and contains following composition: yeast extract paste 7.5g, glucose 10g, peptone 7.5g, potassium primary phosphate 2g, the 100ml of Tomato juice, its pH value is 6.5-7.0.
3, the immobilized cultivation method of " microbiological straw additive " according to claim 1 is characterized in that: " microbiological straw additive " thalline immobilization fermentation condition is: the suitableeest culture temperature is 30 ℃; Optimum pH is 6.0 static fermentation culture.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2008100543464A CN101659951B (en) | 2008-08-29 | 2008-08-29 | Immobilized cultivation method for 'microbiological straw additive' |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2008100543464A CN101659951B (en) | 2008-08-29 | 2008-08-29 | Immobilized cultivation method for 'microbiological straw additive' |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN101659951A true CN101659951A (en) | 2010-03-03 |
| CN101659951B CN101659951B (en) | 2011-10-05 |
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Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN2008100543464A Expired - Fee Related CN101659951B (en) | 2008-08-29 | 2008-08-29 | Immobilized cultivation method for 'microbiological straw additive' |
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| CN (1) | CN101659951B (en) |
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102839125A (en) * | 2012-09-19 | 2012-12-26 | 天津市农业生物技术研究中心 | Solid fermentation method of straw fermentation bio-additive and application |
| CN104560938A (en) * | 2013-10-29 | 2015-04-29 | 中国石油化工股份有限公司 | Preparation method and application of petroleum-degrading bacteria immobilized embedded particles |
| CN107384842A (en) * | 2017-09-22 | 2017-11-24 | 广东工业大学 | A kind of composite bacteria and its application, composite bacteria agent |
| CN109851305A (en) * | 2019-02-14 | 2019-06-07 | 郭绪平 | A kind of concrete repair method for preparing mortar |
-
2008
- 2008-08-29 CN CN2008100543464A patent/CN101659951B/en not_active Expired - Fee Related
Cited By (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102839125A (en) * | 2012-09-19 | 2012-12-26 | 天津市农业生物技术研究中心 | Solid fermentation method of straw fermentation bio-additive and application |
| CN102839125B (en) * | 2012-09-19 | 2014-11-19 | 天津市农业生物技术研究中心 | Solid fermentation method of straw fermentation bio-additive and application |
| CN104560938A (en) * | 2013-10-29 | 2015-04-29 | 中国石油化工股份有限公司 | Preparation method and application of petroleum-degrading bacteria immobilized embedded particles |
| CN104560938B (en) * | 2013-10-29 | 2018-01-16 | 中国石油化工股份有限公司 | A kind of preparation method and applications of immobilizing oil degradation bacteria embedded particles |
| CN107384842A (en) * | 2017-09-22 | 2017-11-24 | 广东工业大学 | A kind of composite bacteria and its application, composite bacteria agent |
| CN109851305A (en) * | 2019-02-14 | 2019-06-07 | 郭绪平 | A kind of concrete repair method for preparing mortar |
| CN109851305B (en) * | 2019-02-14 | 2021-02-23 | 成都顺佳美恒新材料有限公司 | Preparation method of concrete repair mortar |
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| Publication number | Publication date |
|---|---|
| CN101659951B (en) | 2011-10-05 |
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Owner name: TIANJIN CITY LIVESTOCK RAISING AND VETERINARY INST Free format text: FORMER OWNER: TIANJIN CITY AGRICULTURAL BIO-TECH RESEARCH CENTER Effective date: 20130225 |
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Free format text: CORRECT: ADDRESS; FROM: 300384 NANKAI, TIANJIN TO: 300381 XIQING, TIANJIN |
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Effective date of registration: 20130225 Address after: 300381, Tianjin, Xiqing District, 17 kilometers north of Jing Jing, Tianjin agricultural hi tech Demonstration Park Patentee after: TIANJIN INSTITUTE OF ANIMAL HUSBANDRY AND VETERINARY Address before: 300384 17 kilometers, Tianjin Jing Jing highway Patentee before: Tianjin Agricultural Bio-Tech Research Center |
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Effective date of registration: 20210105 Address after: 300192, 26 Nankai District Aerospace Road, Tianjin Patentee after: Tianjin Academy of Agricultural Sciences Address before: 300381 in Tianjin agricultural high tech demonstration park, 17km away from Jinjing, Xiqing District, Tianjin Patentee before: TIANJIN INSTITUTE OF ANIMAL HUSBANDRY AND VETERINARY |
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Granted publication date: 20111005 Termination date: 20210829 |
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