CN102992493A - Improved combined microbe repairing agent for aquaculture environment regulation - Google Patents
Improved combined microbe repairing agent for aquaculture environment regulation Download PDFInfo
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- CN102992493A CN102992493A CN201210401791XA CN201210401791A CN102992493A CN 102992493 A CN102992493 A CN 102992493A CN 201210401791X A CN201210401791X A CN 201210401791XA CN 201210401791 A CN201210401791 A CN 201210401791A CN 102992493 A CN102992493 A CN 102992493A
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- 238000009360 aquaculture Methods 0.000 title abstract description 12
- 244000144974 aquaculture Species 0.000 title abstract description 12
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims abstract description 32
- 241000894006 Bacteria Species 0.000 claims abstract description 26
- 239000003795 chemical substances by application Substances 0.000 claims abstract description 26
- JVTAAEKCZFNVCJ-UHFFFAOYSA-N lactic acid Chemical compound CC(O)C(O)=O JVTAAEKCZFNVCJ-UHFFFAOYSA-N 0.000 claims abstract description 16
- 230000000813 microbial effect Effects 0.000 claims abstract description 12
- 235000014655 lactic acid Nutrition 0.000 claims abstract description 8
- 230000000243 photosynthetic effect Effects 0.000 claims abstract description 8
- 239000003085 diluting agent Substances 0.000 claims abstract description 4
- 150000003242 quaternary ammonium salts Chemical class 0.000 claims abstract description 4
- 244000005700 microbiome Species 0.000 claims description 23
- 238000009418 renovation Methods 0.000 claims description 22
- 239000013543 active substance Substances 0.000 claims description 17
- GVGDNMTUZUVSLQ-UHFFFAOYSA-M C(CCCCCCCCCCC)(=O)[O-].[Mg+] Chemical compound C(CCCCCCCCCCC)(=O)[O-].[Mg+] GVGDNMTUZUVSLQ-UHFFFAOYSA-M 0.000 claims description 3
- 238000002156 mixing Methods 0.000 claims description 3
- FSYKKLYZXJSNPZ-UHFFFAOYSA-N sarcosine Chemical compound C[NH2+]CC([O-])=O FSYKKLYZXJSNPZ-UHFFFAOYSA-N 0.000 claims description 3
- 230000001105 regulatory effect Effects 0.000 claims 1
- 238000002360 preparation method Methods 0.000 abstract description 10
- QVGXLLKOCUKJST-UHFFFAOYSA-N atomic oxygen Chemical compound [O] QVGXLLKOCUKJST-UHFFFAOYSA-N 0.000 abstract description 6
- 230000000694 effects Effects 0.000 abstract description 6
- 229910052760 oxygen Inorganic materials 0.000 abstract description 6
- 239000001301 oxygen Substances 0.000 abstract description 6
- 238000004519 manufacturing process Methods 0.000 abstract description 4
- 230000001580 bacterial effect Effects 0.000 abstract description 2
- 238000000354 decomposition reaction Methods 0.000 abstract description 2
- 239000004094 surface-active agent Substances 0.000 abstract 3
- 244000063299 Bacillus subtilis Species 0.000 abstract 1
- 235000014469 Bacillus subtilis Nutrition 0.000 abstract 1
- KWIUHFFTVRNATP-UHFFFAOYSA-N Betaine Natural products C[N+](C)(C)CC([O-])=O KWIUHFFTVRNATP-UHFFFAOYSA-N 0.000 abstract 1
- 241000222120 Candida <Saccharomycetales> Species 0.000 abstract 1
- 102000004190 Enzymes Human genes 0.000 abstract 1
- 108090000790 Enzymes Proteins 0.000 abstract 1
- KWIUHFFTVRNATP-UHFFFAOYSA-O N,N,N-trimethylglycinium Chemical compound C[N+](C)(C)CC(O)=O KWIUHFFTVRNATP-UHFFFAOYSA-O 0.000 abstract 1
- 229960003237 betaine Drugs 0.000 abstract 1
- 235000019864 coconut oil Nutrition 0.000 abstract 1
- 239000003240 coconut oil Substances 0.000 abstract 1
- 239000002131 composite material Substances 0.000 abstract 1
- 239000004310 lactic acid Substances 0.000 abstract 1
- BJZBHTNKDCBDNQ-UHFFFAOYSA-L magnesium;dodecanoate Chemical compound [Mg+2].CCCCCCCCCCCC([O-])=O.CCCCCCCCCCCC([O-])=O BJZBHTNKDCBDNQ-UHFFFAOYSA-L 0.000 abstract 1
- 239000003876 biosurfactant Substances 0.000 description 4
- 241001465754 Metazoa Species 0.000 description 3
- 230000015572 biosynthetic process Effects 0.000 description 3
- 241000251468 Actinopterygii Species 0.000 description 2
- 230000015556 catabolic process Effects 0.000 description 2
- 238000006243 chemical reaction Methods 0.000 description 2
- 238000006731 degradation reaction Methods 0.000 description 2
- 230000006866 deterioration Effects 0.000 description 2
- JEGUKCSWCFPDGT-UHFFFAOYSA-N h2o hydrate Chemical compound O.O JEGUKCSWCFPDGT-UHFFFAOYSA-N 0.000 description 2
- 238000000034 method Methods 0.000 description 2
- 230000002906 microbiologic effect Effects 0.000 description 2
- 239000002957 persistent organic pollutant Substances 0.000 description 2
- 238000001179 sorption measurement Methods 0.000 description 2
- 239000000126 substance Substances 0.000 description 2
- 241000604931 Bdellovibrio bacteriovorus Species 0.000 description 1
- 241000186660 Lactobacillus Species 0.000 description 1
- 239000008346 aqueous phase Substances 0.000 description 1
- 239000007864 aqueous solution Substances 0.000 description 1
- 150000001875 compounds Chemical class 0.000 description 1
- 238000012258 culturing Methods 0.000 description 1
- 238000005202 decontamination Methods 0.000 description 1
- 230000003588 decontaminative effect Effects 0.000 description 1
- 201000010099 disease Diseases 0.000 description 1
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 description 1
- 238000009826 distribution Methods 0.000 description 1
- 238000004945 emulsification Methods 0.000 description 1
- 230000002708 enhancing effect Effects 0.000 description 1
- 210000003608 fece Anatomy 0.000 description 1
- 238000009472 formulation Methods 0.000 description 1
- 230000002209 hydrophobic effect Effects 0.000 description 1
- 230000003053 immunization Effects 0.000 description 1
- 238000011081 inoculation Methods 0.000 description 1
- 238000002955 isolation Methods 0.000 description 1
- 229940039696 lactobacillus Drugs 0.000 description 1
- 244000005706 microflora Species 0.000 description 1
- 239000000203 mixture Substances 0.000 description 1
- 238000002703 mutagenesis Methods 0.000 description 1
- 231100000350 mutagenesis Toxicity 0.000 description 1
- 235000016709 nutrition Nutrition 0.000 description 1
- 230000035764 nutrition Effects 0.000 description 1
- 150000002894 organic compounds Chemical class 0.000 description 1
- 239000005416 organic matter Substances 0.000 description 1
- 238000006213 oxygenation reaction Methods 0.000 description 1
- 238000004091 panning Methods 0.000 description 1
- 239000002245 particle Substances 0.000 description 1
- 239000011148 porous material Substances 0.000 description 1
- 239000006041 probiotic Substances 0.000 description 1
- 235000018291 probiotics Nutrition 0.000 description 1
- 239000000523 sample Substances 0.000 description 1
- 239000002689 soil Substances 0.000 description 1
- 239000007787 solid Substances 0.000 description 1
- 239000000243 solution Substances 0.000 description 1
- 230000001954 sterilising effect Effects 0.000 description 1
- 238000004659 sterilization and disinfection Methods 0.000 description 1
- 239000003206 sterilizing agent Substances 0.000 description 1
- 238000005728 strengthening Methods 0.000 description 1
- 239000000758 substrate Substances 0.000 description 1
- 230000002195 synergetic effect Effects 0.000 description 1
- 238000009423 ventilation Methods 0.000 description 1
- 239000011800 void material Substances 0.000 description 1
Landscapes
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Fodder In General (AREA)
Abstract
The invention relates to an improved combined microbe repairing agent for aquaculture environment regulation and belongs to the field of category of microbial preparations. The repairing agent consists of a surfactant and multiple microbes, wherein the surfactant accounts for 5-15wt% of the repairing agent; the surfactant comprises palm quaternary ammonium salt, magnesium laurate and gemini-based coconut oil betaine which are mixed at a ratio of 1:1:1 by adding a diluent; each microbe accounts for the following percent in total mass of the microbes: 20-40 percent of bacillus subtilis, 20-40 percent of lactic acid bacteria, 10-30 percent of candida, 5-10 percent of denitrifying bacteria and 5-10 percent of photosynthetic bacteria. The repairing agent is prepared at a certain ratio, and the composite microbes are uniformly distributed in the aquatic environment, so that dissolved oxygen in water is increased, the dominant bacterial community is rapidly formed in a water body, high-activity biological enzymes are generated, decomposition of residual feeds is accelerated, the growth of harmful bacteria is suppressed, and production of green aquatic products is ensured.
Description
Technical field
The present invention relates to a kind of improved combination microorganism renovation agent for the cultivation water environment regulation and control, it belongs to the category of microbial preparation.
Background technology
The hydrophobicity of middle organic pollutant and organism may be by strong adsorption on the suspended particle surfaces or enter the bed mud soil pores because the residual bait of aquaculture water, animal void excreta, aqueous solution organic concentration is reduced, cause the isolation of microorganism and organic pollutant.Because microorganism can not utilize the organism of extremely low concentration, cause organic microbiological deterioration to stop or slowing down.Therefore, the conclusive influence factor of degradation of organic substances is organic low water solubility in the aquaculture water biological restoration, and this is restricted its bioavailability.But can increase organic solvability by applying tensio-active agent, improve the bioavailability of hardly degraded organic substance, promote organic matter degradation.
Bio-surfactant has strengthened organic emulsification degree, promoted its distribution at aqueous phase, namely formed the molten phenomenon of so-called vacation, thereby be conducive to the microbiological deterioration organism, simultaneously, bio-surfactant has also changed the surface properties of microorganism cells, so that microorganism is easy to approach with hydrophobic organic compound or microorganism strengthens the Contact-sorption ability on solid dielectric surface, utilize organic ability thereby finally reach the enhancing microorganism panning, bio-surfactant increases the saturation ratio of oxygen in water body simultaneously.
Microbial preparation about aquaculture water, published patent has " a kind of production method of the lactobacillus micro-ecological preparation for cultivation water regulation ", " a kind of production method of bdellovibrio bacteriovorus ecological preparation ", " a kind of microorganism renovation agent of water environment " and " a kind of probiotics of aquaculture ", " a kind of compound micro-ecological preparation formulation and preparation technology thereof of purifying aquatic water " etc., but utilize tensio-active agent and microbial preparation proportioning to use, at present not report and application, so the applicant has proposed this patent application through on the basis of meticulous experimental study.
Summary of the invention
At present tensio-active agent in aquaculture mostly as sterilization and sterilizing agent, some products are used for substrate improvement and water oxygenation, the present invention is through test of long duration and bacterial classification is selected and the mutagenesis improvement, with tensio-active agent and microbial preparation combination, be combined into a kind of high-effective microorganism renovation agent that improves the aquaculture water environment, can very fast formation superior microorganism population after aquaculture water uses, improve organic ability in the degraded water body, make the very fast improvement of aquaculture water water quality, promote the cultivated animals growth, improve the cultivation surviving rate, increase fanning economics.
The objective of the invention is to be achieved through the following technical solutions:
The improved combination microorganism renovation agent that is used for the cultivation water environment regulation and control, its component is tensio-active agent and multiple-microorganism, wherein, it is 5～15% that tensio-active agent accounts for the renovation agent mass percent, tensio-active agent comprises palm quaternary ammonium salt, Magnesium monolaurate, Shuangzi base coco betaine, and they add mixing diluents in 1: 1: 1 ratio; The mass percent of every kind of microorganism in the microbial total quality is: subtilis 20%～40%, milk-acid bacteria 20～40%, candiyeast 10%～30%, denitrifying bacterium 5～10%, photosynthetic bacterium 5～10%.
Advantage of the present invention and positively effect are:
(1) the present invention adds tensio-active agent in prescription, can significantly improve Dissolved Oxygen in Water, can very fast formation superior microorganism population, improve organic ability in the degraded water body, and make the very fast improvement of aquaculture water water quality.
(2) the present invention adds subtilis, milk-acid bacteria, candiyeast, denitrifying bacterium, photosynthetic bacterium in prescription, can obviously improve cultivation water; Subtilis can produce bio-surfactant, improves culture efficiency, has both had the effect of strengthening the nutrition of fish body, can improve again the immunizing power of body, thereby obviously promote the fish bulk-growth, improves the cultivation surviving rate.Studies show that and to improve 3～5% growth rate that the cultivation surviving rate improves 7～10%.
Embodiment
Further specify the present invention below in conjunction with specific implementation method.
A kind of improved combination microorganism renovation agent for cultivation water environment regulation and control belongs to the category of microbial preparation.This renovation agent is comprised of tensio-active agent and multiple-microorganism, wherein, it is 5～15% that tensio-active agent accounts for the renovation agent mass percent, and tensio-active agent comprises palm quaternary ammonium salt, Magnesium monolaurate, Shuangzi base coco betaine, and they add mixing diluents in 1: 1: 1 ratio; The mass percent of every kind of microorganism in the microbial total quality is: subtilis 20%～40%, milk-acid bacteria 20～40%, candiyeast 10%～30%, denitrifying bacterium 5～10%, photosynthetic bacterium 5～10%.The synergistic function of each component is brought into play in by a certain percentage assembly of renovation agent of the present invention, guarantees that complex microorganism evenly distributes in cultivation water environment, improves the ability of purifying aquaculture water quality.Above-mentioned renovation agent has strong adaptability, drop in the water and can fully distribute at water layer up and down, dissolved oxygen in water is increased, be of value to very fast formation dominant microflora in the water body, and can produce high-activity bio-enzyme, accelerate the decomposition of residual bait and animal excrement, simultaneously can produce microbiotic sample material, suppress the growth of harmful bacteria, reduce the generation of disease, guarantee the production of green aquatic product.
The concrete proportioning mode of mentioned microorganism renovation agent can be:
(1) to account for the renovation agent mass percent be 5% to tensio-active agent, the mass percent of various microorganisms in the microbial total quality is: subtilis 30%, milk-acid bacteria 30%, candiyeast 10%～30%, denitrifying bacterium 5～10%, photosynthetic bacterium 5～10%.
(2) to account for the renovation agent mass percent be 10% to tensio-active agent, the mass percent of various microorganisms in the microbial total quality is: subtilis 30%, milk-acid bacteria 40%, candiyeast 10%～20%, denitrifying bacterium 5～10%, photosynthetic bacterium 5～10%.
(3) to account for the renovation agent mass percent be 15% to tensio-active agent, and the mass percent of various microorganisms in the microbial total quality is: subtilis 40%, milk-acid bacteria 30%, candiyeast 15%, denitrifying bacterium 5～10%, photosynthetic bacterium 5～10%.
Before test showed this renovation agent of use, the oxygen dissolving value of water body was 3.82～4.96mg/L at the bottom of the pond.Add this renovation agent in the pool water within, concentration is 1mg/L, and the water body dissolved oxygen is increased to 5.38～6.24mg/L and only needs 10 minutes, and the time length can reach 2 days.
In the technique scheme, this renovation agent has wider subject range to envrionment conditions, can grow in natural lighting or artificial lighting, ventilation or stuffiness, pH 5.5～8.5 scopes.Prove that by the culturing pool inoculation test this renovation agent is to the NO in the water body
2-N, NH
4 +-N, NH
3-N all has significant removal effect, wherein with to NO
2The decontamination effect improving of-N is the most outstanding, all remains on about 55～63% from inoculating 3d to 15d clearance; To NH
4 +The clearance of-N surpasses 15～20% from inoculating 6d to 12d; To NH
3The clearance of-N surpasses 38～45% from 3d to 9d; Clearance to COD surpasses 15～17.9% from inoculating 3d to 15d.
It is emphasized that: to those skilled in the art, can be improved or conversion according to the following describes, and all these improvement and conversion all should belong to the protection domain of claims of the present invention.
Claims (1)
1. one kind is used for the improved combination microorganism renovation agent that cultivation water environment is regulated and control, it is characterized in that: above-mentioned renovation agent component is tensio-active agent and multiple-microorganism, wherein, it is 5～15% that tensio-active agent accounts for the renovation agent mass percent, tensio-active agent comprises palm quaternary ammonium salt, Magnesium monolaurate, Shuangzi base coco betaine, and they add mixing diluents in 1: 1: 1 ratio; The mass percent of every kind of microorganism in the microbial total quality is: subtilis 20%～40%, milk-acid bacteria 20～40%, candiyeast 10%～30%, denitrifying bacterium 5～10%, photosynthetic bacterium 5～10%.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201210401791.XA CN102992493B (en) | 2012-10-22 | 2012-10-22 | A kind of improved combination microorganism renovation agent for cultivation water environment regulation and control |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201210401791.XA CN102992493B (en) | 2012-10-22 | 2012-10-22 | A kind of improved combination microorganism renovation agent for cultivation water environment regulation and control |
Publications (2)
| Publication Number | Publication Date |
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| CN102992493A true CN102992493A (en) | 2013-03-27 |
| CN102992493B CN102992493B (en) | 2018-08-21 |
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| CN201210401791.XA Active CN102992493B (en) | 2012-10-22 | 2012-10-22 | A kind of improved combination microorganism renovation agent for cultivation water environment regulation and control |
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Cited By (9)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103232097A (en) * | 2013-04-15 | 2013-08-07 | 中国水产科学研究院淡水渔业研究中心 | Composite preparation for increasing dissolved oxygen content in pond culture water body |
| CN103667152A (en) * | 2013-12-19 | 2014-03-26 | 云南晋企生物科技有限公司 | Universal microorganism bacterial agent |
| CN103933894A (en) * | 2014-03-12 | 2014-07-23 | 华南理工大学 | Method and composite surfactant for regulating and controlling aquatic water environment |
| CN105062915A (en) * | 2015-07-23 | 2015-11-18 | 湖南康易达绿茵科技有限公司 | Microecological preparation for degrading N and P (nitrogen and phosphorus) |
| CN105481096A (en) * | 2015-12-18 | 2016-04-13 | 刘高志 | Method for biological remediation of copper-containing sewage |
| CN105540853A (en) * | 2015-12-18 | 2016-05-04 | 刘高志 | Bioremediation method for copper-bearing sewage |
| CN107381831A (en) * | 2017-07-25 | 2017-11-24 | 明光市永言水产(集团)有限公司 | A kind of aquaculture system modifying agent |
| CN108358269A (en) * | 2018-02-06 | 2018-08-03 | 浦江县酉泽水产科技有限公司 | The preparation method of tilapia water quality improving agent |
| CN109305744A (en) * | 2018-11-27 | 2019-02-05 | 上海城市水资源开发利用国家工程中心有限公司 | A kind of black and odorous water biological renovation method and system |
Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101147529A (en) * | 2007-10-31 | 2008-03-26 | 无锡中顺生物技术有限公司 | Microecological preparation for aquaculture and its preparation method |
| CN101269884A (en) * | 2008-05-08 | 2008-09-24 | 储卫华 | Microorganism renovation agent of water environment and preparation method thereof |
| CN101353636A (en) * | 2008-09-11 | 2009-01-28 | 中国水产科学研究院南海水产研究所 | Production method of lactobacillus micro-ecological preparation for cultivation water regulation |
| WO2012135129A1 (en) * | 2011-03-29 | 2012-10-04 | Environmentally Sensitive Solutions, Inc. | Gel carrier for releasing active ingredients |
-
2012
- 2012-10-22 CN CN201210401791.XA patent/CN102992493B/en active Active
Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101147529A (en) * | 2007-10-31 | 2008-03-26 | 无锡中顺生物技术有限公司 | Microecological preparation for aquaculture and its preparation method |
| CN101269884A (en) * | 2008-05-08 | 2008-09-24 | 储卫华 | Microorganism renovation agent of water environment and preparation method thereof |
| CN101353636A (en) * | 2008-09-11 | 2009-01-28 | 中国水产科学研究院南海水产研究所 | Production method of lactobacillus micro-ecological preparation for cultivation water regulation |
| WO2012135129A1 (en) * | 2011-03-29 | 2012-10-04 | Environmentally Sensitive Solutions, Inc. | Gel carrier for releasing active ingredients |
Non-Patent Citations (2)
| Title |
|---|
| 尹伦甫: "表面活性剂在水环境改良剂中的应用", 《科学养鱼》 * |
| 高存川、徐春厚: "微生态制剂在水产养殖水质改良中的应用", 《湖北农业科学》 * |
Cited By (11)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103232097A (en) * | 2013-04-15 | 2013-08-07 | 中国水产科学研究院淡水渔业研究中心 | Composite preparation for increasing dissolved oxygen content in pond culture water body |
| CN103232097B (en) * | 2013-04-15 | 2014-04-09 | 中国水产科学研究院淡水渔业研究中心 | Composite preparation for increasing dissolved oxygen content in pond culture water body |
| CN103667152A (en) * | 2013-12-19 | 2014-03-26 | 云南晋企生物科技有限公司 | Universal microorganism bacterial agent |
| CN103933894A (en) * | 2014-03-12 | 2014-07-23 | 华南理工大学 | Method and composite surfactant for regulating and controlling aquatic water environment |
| CN103933894B (en) * | 2014-03-12 | 2015-12-02 | 华南理工大学 | A kind of regulate and control the method for breeding water body environment and complexed surfactant |
| CN105062915A (en) * | 2015-07-23 | 2015-11-18 | 湖南康易达绿茵科技有限公司 | Microecological preparation for degrading N and P (nitrogen and phosphorus) |
| CN105481096A (en) * | 2015-12-18 | 2016-04-13 | 刘高志 | Method for biological remediation of copper-containing sewage |
| CN105540853A (en) * | 2015-12-18 | 2016-05-04 | 刘高志 | Bioremediation method for copper-bearing sewage |
| CN107381831A (en) * | 2017-07-25 | 2017-11-24 | 明光市永言水产(集团)有限公司 | A kind of aquaculture system modifying agent |
| CN108358269A (en) * | 2018-02-06 | 2018-08-03 | 浦江县酉泽水产科技有限公司 | The preparation method of tilapia water quality improving agent |
| CN109305744A (en) * | 2018-11-27 | 2019-02-05 | 上海城市水资源开发利用国家工程中心有限公司 | A kind of black and odorous water biological renovation method and system |
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| Publication number | Publication date |
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| CN102992493B (en) | 2018-08-21 |
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Inventor after: Li Xuejun Inventor after: Zhang Dahong Inventor after: Huang Hui Inventor after: Qiao Wei Inventor after: Chen Sheng Inventor before: Zhang Dahong Inventor before: Xu Jian |
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