CN105712488A - Biological bottom modifying agent special for fresh water and preparation method thereof - Google Patents
Biological bottom modifying agent special for fresh water and preparation method thereof Download PDFInfo
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- CN105712488A CN105712488A CN201610068005.7A CN201610068005A CN105712488A CN 105712488 A CN105712488 A CN 105712488A CN 201610068005 A CN201610068005 A CN 201610068005A CN 105712488 A CN105712488 A CN 105712488A
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- bacterium solution
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- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F3/00—Biological treatment of water, waste water, or sewage
- C02F3/34—Biological treatment of water, waste water, or sewage characterised by the microorganisms used
- C02F3/347—Use of yeasts or fungi
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- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F3/00—Biological treatment of water, waste water, or sewage
- C02F3/34—Biological treatment of water, waste water, or sewage characterised by the microorganisms used
- C02F3/341—Consortia of bacteria
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- Biodiversity & Conservation Biology (AREA)
- Hydrology & Water Resources (AREA)
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- Environmental & Geological Engineering (AREA)
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Abstract
The invention relates to the field of biotechnologies in freshwater aquaculture, in particular to a biological bottom modifying agent special for fresh water. The biological bottom modifying agent is prepared from, by mass, 60-70 parts of humic acid, 4-8 parts of sodium hydroxide, 6-28 parts of zeolite powder, 6-12 parts of bacillus liquid, 1-2 parts of lactobacillus liquid and 1-2 parts of yeast fungus liquid. The invention further relates to a preparation method of the biological bottom modifying agent special for the fresh water. The method comprises the steps of material taking, dry material mixing, bacterial liquid mixing and granulating. The invention aims at providing the biological bottom modifying agent special for the fresh water and the preparation method thereof. The biological bottom modifying agent can be prepared into bottom modifying granules which are uniform in granule, bright in color and luster and free of dust, bacterial proportion is stable, the biological bottom modifying agent is long in action time after reaching the bottom of a pond, the activity of various microorganism bacterial species can be well guaranteed, the synergistic effect of main components in the product can be well achieved, and a good bottom modifying effect is achieved.
Description
Technical field
The present invention relates to the biological technical field in freshwater aquiculture, particularly relate to change agent and preparation method thereof at the bottom of a kind of fresh water special bio.
Background technology
Breeding fish and first support water, good water has supported fish, and along with the raising of the intensive culture degree of aquaculture, water quality also goes from bad to worse, and difficult disease increases, and water quality management becomes the problem that culturist pays close attention to the most.Along with the growth of cultivation phase, the Organic substance in pond gets more and more, along with the rising of water temperature, whole during constantly bait throwing in, Fish constantly ingest, and constantly drain, and the algae in water constantly grows, dead.Cultivation density is too high, cause and throw something and feed too much, substantial amounts of residual bait, feces, Excreta diffusion dissolution neutralize in water and are deposited on the bottom, unnecessary feedstuff, fish and shrimp Excreta, dead algae also has some other vegeto-animal corpse etc. all to accumulate in water, cultivation is carried out day by day, these materials accumulate the most day by day, running up to a certain degree etc. total amount, cause the pH value of water body to change, water body ammonia nitrogen, content of nitrite rise, water transparency declines, Organic substance amount reproduction and the variation of algae phase, water quality entirety deteriorates, and bacterial pathogens grows.
The water quality management in fishpond, directly affects benefit of breeding fish.The most employing chemistry water purification agents and biologic water cleaner carry out purification of water quality.Chemistry water purification agent is that chemosorbent, chelating agent, oxygenation agent, disinfectant, reducing agent, high molecular polymer etc. detoxify by chemical reaction, main based on chemicals, such as: aluminium polychlorid, bodied ferric sulfate, aluminum sulfate, polyacrylamide etc., its action principle is the harmful substance in flocculation water body so that it is sink at the bottom of pond after combining with self.But although harmful substance decreases in water body, but because flocculation to the harmful substance at the bottom of pond too much can not be removed, if being repeatedly used for a long this water purification agent, also can cause self metallics and the chemical substance secondary pollution to water body, therefore its effect can only alleviate the pollution of water body, it is impossible to fundamentally removes pollution.Biologic water cleaner can ammonia nitrogen in a certain degree of removal water and nitrite, improve water pollution, hence with microorganism formulation pollution control of water by people's common concern, but there is following defect in existing biologic water cleaner:
1, bacterial strain uses therefor is not through specific exercises, at pond bottom, flourish inclement condition, lacks activation mechanism, it is impossible to play right effect；
2, melt water is fast, arrives pond bottom after effect time short,
3, effect is single, only realizes being deposited to strain the effect of pond bottom, fails to realize the synergism between product main constituent；
4, strain mixing inequality, binding agent adding proportion is too high, and granule color and luster does not works, and size is uneven.
By patent retrieval, there is following known prior art:
Patent 1:
Application number: 201010160244.8, the applying date: 2010-04-29, authorized announcement date: 2010-09-15, the invention discloses a kind of method preventing and treating cyanophyceae and inorganic agent.It is of the present invention that to bite phycomycete S agent be the culture fluid that Spirulina powder, NaCl, pure water are made into solid content 0.5 ~ 2%；It is to be compounded formed by yeast, bacillus subtilis, bacteriophagic Bdellovibrio that the described biological end changes agent K agent preparation；Described algae-removing water-purifying agent L agent preparation be weight ratio be SODIUM PERCARBONATE: forth generation polyamide one amine: sodium bentonite is to constitute at 40%: 50%: 10%.What the present invention solved that the cyanophyceae contention probiotics of auxotype and possessing swallows cyanophyceae bites the defect that two kinds of methods of phycomycete are individually present.The present invention is that bioanalysis organically combines the anti-of formation with chemical method and controls cyanophyceae system engineering, treating both the principal and secondary aspects of a disease of the present invention, cost-effective, possesses the good suitability and stability；Cyanophyceae prevention and control are lived by early stage microbial method, and the cell wall ratio utilizing cyanophyceae is relatively thin, bite phycomycete phagocytosis algae soon, and implementation result is obvious, and summer, water body eutrophication degree was high, and effect of integrated pest management is good.
Patent 2:
Application number: 201210510924.7, the applying date: 2012-12-04, Shen Qing Publication day:
2013-03-13, the invention discloses a kind of Biological sediment modifier for fishpond, belongs to fishpond water quality purification techniques field.It is made up of the raw material of following weight proportioning: bacillus subtilis mycopowder 3-8 part, nitrobacteria mycopowder 3-8 part, denitrifying bacteria mycopowder 3-8 part, sulfide-oxidizing enzyme mycopowder 3-8 part, zeolite powder 10-40 part, sodium humate 40-70 part, calper calcium peroxide 0.5-3 part, its overall viable bacteria content 2 × 108-2 × 109/g.The present invention can absorb bed mud nutrition, by fermenting, breed, expanding flora, forms Mycoderma and covered by bed mud on bed mud, make it can not polluted water, it is beneficial to the growth of aquatic animal, substantially increases degradation rate, stopped the secondary pollution that water body is caused by chemical degradation.
Patent 3:
Application number: 201410411845.X, the applying date: 2014-08-21, Shen Qing Publication day:
2014-12-03, the invention discloses and change agent at the bottom of a kind of fresh water complex microorganism, belong to the biological technical field in freshwater aquiculture.It is to mix with Bafillus natt mycopowder, lactic acid bacteria mycopowder, clostridium butyricum mycopowder, biological flocculant and regenerative agent that this end changes agent, stirs and forms.The end of the present invention, changes agent and utilizes the flocculation of biological flocculant itself by after the organic pollution flocculation formation bed mud in breeding water body, by the organic-flocculation thing in the intensive decomposition action breaks bed mud of aerobic bacteria, facultative anaerobe and strict anaerobes, thus improve freshwater aquiculture Sediments quality, there is Biodegradable and safety and efficient, nontoxic, the feature of non-secondary pollution.
Being found by above retrieval, above technical scheme can not affect the novelty of the present invention；And above patent document be mutually combined the creativeness that can not destroy the present invention.
Summary of the invention
It is an object of the invention to provide and change agent and preparation method thereof at the bottom of a kind of fresh water special bio, can make that granule is uniform, the bright color non-dusting end changes agent granule, wherein strain ratio is stable, reach pond bottom after effect time longer, and can be good at ensureing the activity of each microorganism fungus kind, and can be good at realizing the synergism between product main constituent, the end reaching good, changes effect.
In order to realize object above, the technical solution used in the present invention is: change agent at the bottom of a kind of fresh water special bio, and it includes the raw material of following mass fraction: humic acid 60-70 part；Sodium hydroxide 4-8 part；Zeolite powder 6-28 part；Bacillus cereus bacterium solution 6-12 part；Lactobacillus bacterium solution 1-2 part；Yeast bacterium solution 1-2 part.
Preferably, described bacillus cereus bacterium solution includes can be low temperature bacillus subtilis bacterium solution 3-6 part of 8 DEG C of activity maintained above and the antibacterial bacillus cereus bacterium solution 3-6 part that can suppress vibrio growth promoter through screening through screening.
Preferably, in described low temperature bacillus subtilis bacterium solution, the content of low temperature bacillus subtilis is not less than 50 × 108Individual/mL；In described antibacterial bacillus cereus bacterium solution, the content of antibacterial bacillus cereus is not less than 50 × 108Individual/mL.
Preferably, described lactobacillus bacterium solution is bacillus acidophilus's bacterium solution, and bacillus acidophilus's content therein is no less than 3 × 108Individual/mL.
Preferably, described yeast liquid is KShi yeast liquid, and wherein the saccharomycetic content of KShi is no less than 3 × 108Individual/mL.
Another scheme of the present invention: change the preparation method of agent at the bottom of a kind of fresh water special bio, it is characterised in that it comprises the steps:
1., feeding, weigh various raw material according to formula；
2., siccative mixes, by humic acid, sodium hydroxide and zeolite powder mix homogeneously；
3., bacterium solution mixing, whole microbial inoculum mix homogeneously that step 1. material step is taken, and by mixing access Sprinkler in；
4., pelletizing, mixture step 2. obtained is poured in granulating disc, and sprays into, during rotating, the bacterium solution mixed liquor that 3. step is prepared, and the classified sieve of granule of formation, polishing disk obtain changing at the bottom of fresh water special bio agent finished product after processing.
The invention have the benefit that
1, using sodium hydroxide as binding agent, decrease the adding proportion of zeolite powder, the viscosity of product is more moderate, and the melt water time is 2-12h, can preferably play the effect of each microbial activity composition.
2, product is made the granule of bright in color, and granule is uniform, both ensure that the end changed the attractive in appearance of agent, can be good at when incorporating in water making a microorganism fungus kind produce synergism simultaneously.
3, bacterial strain is reasonably combined: use low temperature bacillus subtilis (>=50 × 108/mL), bacillus acidophilus (>=3 × 108/mL), antibacterial bacillus cereus bacillus (>=50 × 108/mL) and KShi yeast (>=3 × 108/mL).The introducing of low temperature bacillus cereus can ensure that water temperature can play a role more than 8 DEG C, solves the problem that the low strain of pond bottom temperature starts in early days；Growing of harmful bacteria is killed or is inhibited in the introducing of antibacterial bacillus cereus, accelerates supplemented strain and is formed with the advantage of bacteria group；Spore, lactic acid bacteria and saccharomycetic synergism, large amount of organic bottom degraded, obtains the available compositions such as a large amount of small-molecule substances rapidly.
4, preparation method step is simple, and uses single bacterium fermentation, it is ensured that the purity of 100%, being mixed in proportion before interpolation, added by hydrojet mode, operation more simplifies, detect convenient, the uniform depth of strain can be reacted by detection product water content really, adjust in good time.
Detailed description of the invention
Understandable for enabling the above-mentioned purpose of the present invention, feature and advantage to become apparent from, below the detailed description of the invention of the present invention is elaborated.Elaborate a lot of detail in the following description so that fully understanding the present invention.But the present invention can implement to be much different from other modes described here, those skilled in the art can do similar improvement in the case of intension of the present invention, and therefore the present invention is not limited by following public being embodied as.
The following is specific embodiment
Embodiment 1:
Changing agent at the bottom of a kind of fresh water special bio, it includes the raw material of following mass fraction: humic acid 60 parts；Sodium hydroxide 4 parts；Zeolite powder 28 parts；Low temperature bacillus subtilis bacterium solution 3 parts；Bacillus acidophilus's bacterium solution 2 parts；Antibacterial bacillus cereus 3 parts；KShi yeast bacterium solution 2 parts.
Changing the preparation method of agent at the bottom of a kind of fresh water special bio, it comprises the steps:
1., feeding, weigh various raw material according to formula；
2., siccative mixes, by humic acid, sodium hydroxide and zeolite powder mix homogeneously；
3., bacterium solution mixing, whole microbial inoculum mix homogeneously that step 1. material step is taken, and by mixing access Sprinkler in；
4., pelletizing, mixture step 2. obtained is poured in granulating disc, and sprays into, during rotating, the bacterium solution mixed liquor that 3. step is prepared, and the classified sieve of granule of formation, polishing disk obtain changing at the bottom of fresh water special bio agent finished product after processing.
Embodiment 2:
Changing agent at the bottom of a kind of fresh water special bio, it includes the raw material of following mass fraction: humic acid 70 parts；Sodium hydroxide 8 parts；Zeolite powder 6 parts；Low temperature bacillus subtilis bacterium solution 6 parts；Bacillus acidophilus's bacterium solution 1 part；Antibacterial bacillus cereus 6 parts；KShi yeast bacterium solution 1 part.
Changing the preparation method of agent at the bottom of a kind of fresh water special bio, it comprises the steps:
1., feeding, weigh various raw material according to formula；
2., siccative mixes, by humic acid, sodium hydroxide and zeolite powder mix homogeneously；
3., bacterium solution mixing, whole microbial inoculum mix homogeneously that step 1. material step is taken, and by mixing access Sprinkler in；
4., pelletizing, mixture step 2. obtained is poured in granulating disc, and sprays into, during rotating, the bacterium solution mixed liquor that 3. step is prepared, and the classified sieve of granule of formation, polishing disk obtain changing at the bottom of fresh water special bio agent finished product after processing.
Embodiment 3:
Changing agent at the bottom of a kind of fresh water special bio, it includes the raw material of following mass fraction: humic acid 65 parts；Sodium hydroxide 6 parts；Zeolite powder 20 parts；Low temperature bacillus subtilis bacterium solution 4 parts；Bacillus acidophilus's bacterium solution 2 parts；Antibacterial bacillus cereus 5 parts；KShi yeast bacterium solution 1 part.
Changing the preparation method of agent at the bottom of a kind of fresh water special bio, it comprises the steps:
1., feeding, weigh various raw material according to formula；
2., siccative mixes, by humic acid, sodium hydroxide and zeolite powder mix homogeneously；
3., bacterium solution mixing, whole microbial inoculum mix homogeneously that step 1. material step is taken, and by mixing access Sprinkler in；
4., pelletizing, mixture step 2. obtained is poured in granulating disc, and sprays into, during rotating, the bacterium solution mixed liquor that 3. step is prepared, and the classified sieve of granule of formation, polishing disk obtain changing at the bottom of fresh water special bio agent finished product after processing.
Checking:
Changing agent by the bottom of the fresh water special bio that the embodiment of the present invention 1, embodiment 2 and embodiment 3 prepare, its granule is uniform, bright in color, and without dust, in granule, strain is stable, and content is preferable, it is ensured that value is 3 × 108/g.And being carried out water entry test, effectiveness is preferable, and the time of melt water, all in 2-12h, reaches more than 8 DEG C strains in pond bottom temperature and just can play a role, and after onset, synergism ability is strong, is affected little by dissolved oxygen, organic components, and degraded producing level is high.
It should be noted that, in this article, term " includes ", " comprising " or its any other variant are intended to comprising of nonexcludability, so that include that the process of a series of key element, method, article or equipment not only include those key elements, but also include other key elements being not expressly set out, or also include the key element intrinsic for this process, method, article or equipment.
Principle and the embodiment of the present invention are set forth by specific case used herein, and the explanation of above example is only intended to help to understand method and the core concept thereof of the present invention.The above is only the preferred embodiment of the present invention, should be understood that, finiteness due to literal expression, and objectively there is unlimited concrete structure, for those skilled in the art, under the premise without departing from the principles of the invention, it is also possible to make some improvement, retouch or change, it is also possible to above-mentioned technical characteristic is combined by rights；These improve retouching, change or combine, or the most improved design by invention and technical scheme directly apply to other occasion, are regarded as protection scope of the present invention.
Claims (6)
1. change agent at the bottom of a fresh water special bio, it is characterised in that it includes the raw material of following mass fraction: humic acid 60-70 part；Sodium hydroxide 4-8 part；Zeolite powder 6-28 part；Bacillus cereus bacterium solution 6-12 part；Lactobacillus bacterium solution 1-2 part；Yeast bacterium solution 1-2 part.
Agent is changed at the bottom of a kind of fresh water special bio the most according to claim 1, it is characterized in that, described bacillus cereus bacterium solution includes can be low temperature bacillus subtilis bacterium solution 3-6 part of 8 DEG C of activity maintained above and the antibacterial bacillus cereus bacterium solution 3-6 part that can suppress vibrio growth promoter through screening through screening.
Agent is changed, it is characterised in that in described low temperature bacillus subtilis bacterium solution, the content of low temperature bacillus subtilis is not less than 50 × 10 at the bottom of a kind of fresh water special bio the most according to claim 28Individual/mL；In described antibacterial bacillus cereus bacterium solution, the content of antibacterial bacillus cereus is not less than 50 × 108Individual/mL.
Agent is changed, it is characterised in that described lactobacillus bacterium solution is bacillus acidophilus's bacterium solution, and bacillus acidophilus's content therein is no less than 3 × 10 at the bottom of a kind of fresh water special bio the most according to claim 18Individual/mL.
Agent is changed, it is characterised in that described yeast liquid is KShi yeast liquid, and wherein the saccharomycetic content of KShi is no less than 3 × 10 at the bottom of a kind of fresh water special bio the most according to claim 18Individual/mL.
6. as at the bottom of any one fresh water special bio in the claims 1-5, change the preparation method of agent, it is characterised in that it comprises the steps:
1., feeding, weigh various raw material according to formula；
2., siccative mixes, by humic acid, sodium hydroxide and zeolite powder mix homogeneously；
3., bacterium solution mixing, whole microbial inoculum mix homogeneously that step 1. material step is taken, and by mixing access Sprinkler in；
4., pelletizing, mixture step 2. obtained is poured in granulating disc, and sprays into, during rotating, the bacterium solution mixed liquor that 3. step is prepared, and the classified sieve of granule of formation, polishing disk obtain changing at the bottom of fresh water special bio agent finished product after processing.
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Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107285485A (en) * | 2017-08-21 | 2017-10-24 | 苏州神良生物科技有限公司 | A kind of freshwater aquiculture biological regulator |
| CN110050904A (en) * | 2018-01-17 | 2019-07-26 | 厦门海约生物科技有限公司 | A kind of fulvic acid used for aquiculture and lactobacillus fermentation material and preparation method thereof |
| CN110078227A (en) * | 2019-06-03 | 2019-08-02 | 无锡三智生物科技有限公司 | A kind of microorganism water priming agent and preparation method |
| CN111573850A (en) * | 2020-05-08 | 2020-08-25 | 马鞍山市徽农农业科技发展有限公司 | Substrate modifier for river crab high-temperature period culture and preparation method thereof |
| CN115432827A (en) * | 2022-07-28 | 2022-12-06 | 江西多优生物科技有限公司 | Biological bottom modifying agent and preparation method thereof |
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| CN102864137A (en) * | 2012-09-26 | 2013-01-09 | 中国海洋大学 | Microbial particle preparation for aquacultural water quality purification and preparation method |
| CN103181461A (en) * | 2011-12-27 | 2013-07-03 | 北京资源亚太饲料科技有限公司 | Composite microecological preparation for feed and preparation method thereof |
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| CN101054229A (en) * | 2007-04-04 | 2007-10-17 | 上海环境工程技术有限公司 | Alveolate carrier deposited with stale garbage microorganism, preparation method and application thereof |
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Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107285485A (en) * | 2017-08-21 | 2017-10-24 | 苏州神良生物科技有限公司 | A kind of freshwater aquiculture biological regulator |
| CN110050904A (en) * | 2018-01-17 | 2019-07-26 | 厦门海约生物科技有限公司 | A kind of fulvic acid used for aquiculture and lactobacillus fermentation material and preparation method thereof |
| CN110078227A (en) * | 2019-06-03 | 2019-08-02 | 无锡三智生物科技有限公司 | A kind of microorganism water priming agent and preparation method |
| CN111573850A (en) * | 2020-05-08 | 2020-08-25 | 马鞍山市徽农农业科技发展有限公司 | Substrate modifier for river crab high-temperature period culture and preparation method thereof |
| CN115432827A (en) * | 2022-07-28 | 2022-12-06 | 江西多优生物科技有限公司 | Biological bottom modifying agent and preparation method thereof |
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| CN105712488B (en) | 2018-06-15 |
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Effective date of registration: 20190603 Address after: 415600 Donghe Street, Yongle Community, Anxiang County, Changde City, Hunan Province (Jinmei Taurus 100m) Patentee after: Hunan Zhengyuan Feed Co., Ltd. Address before: 415600 Dongting South Road, Changlingzhou Community, Shenliu Town, Anxiang County, Changde City, Hunan Province (Industrial Park) Patentee before: North of Hunan bio tech ltd, Hunan |
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Granted publication date: 20180615 Termination date: 20210201 |