CN116515719A - Microbial composite microbial agent for water quality improvement, preparation method and application thereof - Google Patents

Microbial composite microbial agent for water quality improvement, preparation method and application thereof Download PDF

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CN116515719A
CN116515719A CN202310800398.6A CN202310800398A CN116515719A CN 116515719 A CN116515719 A CN 116515719A CN 202310800398 A CN202310800398 A CN 202310800398A CN 116515719 A CN116515719 A CN 116515719A
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microbial
bacteria
agent
water quality
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韩赛虎
万小平
贺琦
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China Construction Yitong Technology Co ltd
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China Construction Yitong Technology Co ltd
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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/20—Bacteria; Culture media therefor
    • C—CHEMISTRY; METALLURGY
    • C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
    • C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
    • C02F1/00—Treatment of water, waste water, or sewage
    • C02F1/50—Treatment of water, waste water, or sewage by addition or application of a germicide or by oligodynamic treatment
    • C—CHEMISTRY; METALLURGY
    • C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
    • C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
    • C02F3/00—Biological treatment of water, waste water, or sewage
    • C02F3/32—Biological treatment of water, waste water, or sewage characterised by the animals or plants used, e.g. algae
    • C02F3/322—Biological treatment of water, waste water, or sewage characterised by the animals or plants used, e.g. algae use of algae
    • C02F3/325—Biological treatment of water, waste water, or sewage characterised by the animals or plants used, e.g. algae use of algae as symbiotic combination of algae and bacteria
    • C—CHEMISTRY; METALLURGY
    • C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
    • C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
    • C02F3/00—Biological treatment of water, waste water, or sewage
    • C02F3/34—Biological treatment of water, waste water, or sewage characterised by the microorganisms used
    • C02F3/348—Biological treatment of water, waste water, or sewage characterised by the microorganisms used characterised by the way or the form in which the microorganisms are added or dosed
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/12—Unicellular algae; Culture media therefor
    • C—CHEMISTRY; METALLURGY
    • C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
    • C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
    • C02F2101/00—Nature of the contaminant
    • C02F2101/10—Inorganic compounds
    • C02F2101/105—Phosphorus compounds
    • C—CHEMISTRY; METALLURGY
    • C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
    • C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
    • C02F2101/00—Nature of the contaminant
    • C02F2101/10—Inorganic compounds
    • C02F2101/16—Nitrogen compounds, e.g. ammonia
    • C—CHEMISTRY; METALLURGY
    • C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
    • C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
    • C02F2101/00—Nature of the contaminant
    • C02F2101/30—Organic compounds
    • C—CHEMISTRY; METALLURGY
    • C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
    • C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
    • C02F2303/00—Specific treatment goals
    • C02F2303/04—Disinfection
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12R—INDEXING SCHEME ASSOCIATED WITH SUBCLASSES C12C - C12Q, RELATING TO MICROORGANISMS
    • C12R2001/00—Microorganisms ; Processes using microorganisms
    • C12R2001/01—Bacteria or Actinomycetales ; using bacteria or Actinomycetales
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12R—INDEXING SCHEME ASSOCIATED WITH SUBCLASSES C12C - C12Q, RELATING TO MICROORGANISMS
    • C12R2001/00—Microorganisms ; Processes using microorganisms
    • C12R2001/01—Bacteria or Actinomycetales ; using bacteria or Actinomycetales
    • C12R2001/07—Bacillus
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12R—INDEXING SCHEME ASSOCIATED WITH SUBCLASSES C12C - C12Q, RELATING TO MICROORGANISMS
    • C12R2001/00—Microorganisms ; Processes using microorganisms
    • C12R2001/89—Algae ; Processes using algae
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02W—CLIMATE CHANGE MITIGATION TECHNOLOGIES RELATED TO WASTEWATER TREATMENT OR WASTE MANAGEMENT
    • Y02W10/00—Technologies for wastewater treatment
    • Y02W10/10—Biological treatment of water, waste water, or sewage

Abstract

The invention provides a microbial composite microbial agent for water quality improvement, a preparation method and application thereof, belongs to the technical field of microorganisms, and comprises bacillus amyloliquefaciens, paenibacillus piri, bdellovibrio bacteriovorus, photosynthetic bacteria, EM bacteria and micro-mango algae, and can also comprise carbamide peroxide, chitosan, humic acid and a carrier. The microbial composite microbial agent can greatly reduce the indexes of ammonia nitrogen, total phosphorus and COD in sewage in a short time, improve the transparency of water bodies, purify the water bodies and restore the ecological environment of the water bodies. Can also continuously play a role in the water environment, and can keep the purifying effect for a long time, and the purifying time is as long as 6 months. The method can treat both symptoms and root causes, and solves the technical problems of short purifying time and repeated adding of the water purifying agent in the prior art.

Description

Microbial composite microbial agent for water quality improvement, preparation method and application thereof
Technical Field
The invention belongs to the technical field of microorganisms, and relates to a microbial composite microbial agent for water quality improvement, a preparation method and application thereof.
Background
With the development of large-scale aquaculture and intensive technology, residual baits, excreta and other organic pollutants in ponds are increased, dissolved oxygen is consumed in a large amount for decomposing the organic pollutants, and a large amount of harmful and toxic substances such as ammonia nitrogen, nitrite nitrogen, sulfides and the like are generated. With the increase of the toxic and harmful substances, the growth and the reproduction of aquatic animals are affected, and serious poisoning death is even generated. The quantity of pathogenic microorganisms in the water body is directly related to the concentration of ammonia nitrogen, nitrite nitrogen and sulfide in the water body, for example, one of the morbidity conditions of bacterial septicemia of freshwater fish is that the water body is deteriorated, and the ammonia nitrogen and nitrite nitrogen are obviously higher. Therefore, how to effectively regulate the quality of aquaculture water becomes a key issue in aquaculture.
At present, the application of the microbial preparation in freshwater aquaculture tends to the compound use of multiple strains, and the purpose is to enable the strains with different improvement functions to jointly exert the functions of ammoniation, nitration and the like, so as to make the best of the advantages and the disadvantages, and further to degrade the pollutants in the pond. The degradation effect of a single microbial agent on freshwater aquaculture pollutants is not ideal because the single microbial agent has difficulty in maintaining its competitive advantage throughout the changing aquaculture environment. The various strains in the compound microorganism preparation can properly adjust the structure and composition of the flora along with the change of the water environment through interaction, keep the dynamic activity of the flora and continuously exert the water improvement effect. Test to construct flora according to the degradation rate of different strains and the differential mobility of degradation substrates, and by detecting Chemical Oxygen Demand (COD) and ammoniacal Nitrogen (NH) in water environment 4- N, nitrite nitrogen(NO 2 -N) and dissolved oxygen, etc., in order to screen out high-efficiency mixed flora.
Disclosure of Invention
According to the technical defects existing in the prior art, the technical problems to be solved by the invention are as follows: provides a microbial agent of a microbial composite bacterial agent for improving water quality, a preparation method and application thereof, solves the technical problem that the microbial agent in the prior art has weaker decomposing capability on water pollutants, and protects the water environment.
In order to achieve the technical purpose of the invention, the invention provides a microbial composite microbial agent for water quality improvement, which comprises the following raw materials in parts by weight: bacillus amyloliquefaciens @Bacillus amyloliquefaciens) 6-10 parts of Paenibacillus piriPaenibacillus peoriae) 6-8 parts of bdellovibrio bacteriophagic bacteriaBdellovibrio bacteriouorus) 2-4 parts of photosynthetic bacteria, 3-5 parts of EM bacteria, 4-6 parts of micro-miscanthusMicractinium pusillum) 2-6 parts.
Further, the microbial composite inoculant comprises the following raw materials in parts by weight: bacillus amyloliquefaciens @Bacillus amyloliquefaciens) 10 parts of Paenibacillus piriPaenibacillus peoriae) 8 parts of bdellovibrio bacteriophagic bacteriaBdellovibrio bacteriouorus) 4 parts of photosynthetic bacteria, 3 parts of EM bacteria, 5 parts of micro-mango algaeMicractinium pusillum) 5 parts.
Further, the microbial composite inoculant also comprises the following components: 1-2 parts of carbamide peroxide, 1-5 parts of chitosan and 4-6 parts of humic acid.
Further, the microbial composite inoculant also comprises the following components: 1 part of carbamide peroxide, 3 parts of chitosan and 5 parts of humic acid.
Further, the microbial composite bacterial agent also comprises 100-120 parts of microbial carrier, wherein the microbial carrier is diatomite or wood dust.
The invention also provides a preparation method of the microbial composite microbial agent for water quality improvement, which specifically comprises the following steps:
(1) Preparing bacterial liquid: inoculating activated bacillus amyloliquefaciens, paenibacillus piri, bdellovibrio bacteriovorus, photosynthetic bacteria, EM bacteria and micro-mango algae into a fermentation culture medium respectively, culturing for 24-48h to obtain bacteria/algae liquid, concentrating and drying the bacteria/algae liquid obtained by fermentation.
(2) Compounding of microbial agents: 6-10 parts of bacillus amyloliquefaciens, 6-8 parts of paenibacillus piri, 2-4 parts of bdellovibrio bacteriovorus, 3-5 parts of photosynthetic bacteria, 4-6 parts of EM bacteria and 2-6 parts of micro-mango algae are mixed and compounded according to the parts by weight.
Further, in the step (1), the fermentation medium of the bacillus amyloliquefaciens, the paenibacillus pierce and the EM bacteria comprises the following components: soluble corn starch 67.0g/L, peanut cake dip 41.6g/L, peptone 10g/L, K 2 HPO 4 ·3H 2 O9.2 g/L and MgSO 4 ·7H 2 O 1.5 g/L。
Further, in the step (1), the fermentation medium of Bdellovibrio bacteriovorus comprises the following components: beef extract 3g/L, tryptone 10g/L, yeast extract 5g/L, sodium chloride 10g/L.
Further, in the step (1), the fermentation medium composition of the photosynthetic bacteria is: NH (NH) 4 Cl 1g/L，CH 3 COONa 3.5g/L，MgCl 2 0.1g/L，CaCl 2 0.1g/L，KH 2 PO 4 0.6g/L，K 2 HPO 4 0.4g/L, and yeast extract 0.1g/L.
Further, in the step (1), the fermentation medium of the micro-mango algae is a BG11 medium.
Further, 1-2 parts of carbamide peroxide, 1-5 parts of chitosan, 4-6 parts of humic acid and 100-120 parts of microorganism carrier can be added in the microbial agent compounding of the step (2).
The invention also provides a water quality improvement method, which comprises the step of applying the microbial composite bacterial agent for water quality improvement to sewage.
The invention also provides an application of the microbial composite microbial agent for water quality improvement or the preparation method thereof in water quality improvement.
Compared with the prior art, the invention has the beneficial effects that:
the beneficial bacteria form dominant bacteria in the environment by adding the microbial composite microbial agent into the water body, the microbial ecological structure in the water body is regulated and controlled, harmful substances in the water body are degraded or inhibited, excessive propagation of algae is inhibited, the transparency of the water body is increased, and the purpose of purifying the water body is achieved.
The microbial composite microbial agent can greatly reduce the indexes of ammonia nitrogen, total phosphorus and COD in sewage in a short time, purify water bodies, restore the ecological environment of the water bodies, regulate and adjust the proportion among bacillus amyloliquefaciens, paenibacillus pirillum, bdellovibrio bacteriovorus, photosynthetic bacteria, EM bacteria and micro-mango algae, so that the optimal purification effect is achieved, and the synergistic promotion is realized.
The carbamide peroxide is added in the microbial composite microbial inoculum, is a high-efficiency, safe and convenient-to-use solid disinfectant, and has a disinfection effect superior to that of hydrogen peroxide and peracetic acid solution. He has the advantages of broad sterilization spectrum, strong sterilization capability, low use concentration, short sterilization time, no residual toxicity after sterilization and no irritation. It can inhibit the growth of bacteria and mould, kill common bacteria such as typhoid bacillus, dysentery bacillus, colibacillus, etc. and is favorable to fast purifying water quality.
The microbial composite microbial inoculum can not only rapidly purify water in a short time, but also continuously play a role in the water environment, and long-term maintain the purification effect, and the purification time is as long as 6 months. The method can treat both symptoms and root causes, and solves the technical problems of short purifying time and repeated adding of the water purifying agent in the prior art.
Detailed Description
The following specific examples are set forth to further illustrate the beneficial effects of the present invention, and are specifically described to provide a better understanding of the present invention and are in no way limiting the scope of the present invention. The experimental methods in the following examples are conventional methods unless otherwise specified. The experimental materials used in the following examples, unless otherwise specified, were all conventional biochemical formulations purchased at the store. The quantitative tests in the following examples were all set up in triplicate and the results averaged.
The culture media used in the examples of the present application were:
the fermentation medium of the bacillus amyloliquefaciens, the paenibacillus piri and the EM bacteria comprises the following components: soluble corn starch 67.0g/L, peanut cake dip 41.6g/L, peptone 10g/L, K 2 HPO 4 ·3H 2 O9.2 g/L and MgSO 4 ·7H 2 O 1.5 g/L。
The fermentation medium of Bdellovibrio bacteriovorus comprises the following components: beef extract 3g/L, tryptone 10g/L, yeast extract 5g/L, sodium chloride 10g/L.
The fermentation medium of photosynthetic bacteria comprises the following components: NH (NH) 4 Cl 1g/L，CH 3 COONa 3.5g/L，MgCl 2 0.1g/L，CaCl 2 0.1g/L，KH 2 PO 4 0.6g/L，K 2 HPO 4 0.4g/L, and yeast extract 0.1g/L.
The fermentation medium of the micro-mango algae is a commercial BG11 medium.
The strains used in this application were all purchased commercially.
Example 1
(1) Preparing bacterial liquid: inoculating activated bacillus amyloliquefaciens, paenibacillus piri, bdellovibrio bacteriovorus, photosynthetic bacteria, EM bacteria and micro-mango algae into a fermentation culture medium respectively, culturing for 48 hours to obtain bacteria/algae liquid, concentrating and drying the bacteria/algae liquid obtained by fermentation.
(2) Compounding of microbial agents: and mixing and compounding 10 parts of bacillus amyloliquefaciens, 8 parts of paenibacillus piri, 4 parts of bdellovibrio bacteriovorus, 3 parts of photosynthetic bacteria, 5 parts of EM bacteria and 5 parts of micro-mango algae according to the parts by weight to obtain the microbial compound bacterial agent.
Example 2
(1) Preparing bacterial liquid: inoculating activated bacillus amyloliquefaciens, paenibacillus piri, bdellovibrio bacteriovorus, photosynthetic bacteria, EM bacteria and micro-mango algae into a fermentation culture medium respectively, culturing for 48 hours to obtain bacteria/algae liquid, concentrating and drying the bacteria/algae liquid obtained by fermentation.
(2) Compounding of microbial agents: the dried bacteria/algae are mixed and compounded according to the mass parts of 10 parts of bacillus amyloliquefaciens, 8 parts of paenibacillus piri, 4 parts of bdellovibrio bacteriovorus, 3 parts of photosynthetic bacteria, 5 parts of EM bacteria, 5 parts of micro-mango algae, 1 part of carbamide peroxide, 3 parts of chitosan, 5 parts of humic acid and 100 parts of diatomite, so as to obtain the microbial composite microbial agent.
Example 3
(1) Preparing bacterial liquid: inoculating activated bacillus amyloliquefaciens, paenibacillus piri, bdellovibrio bacteriovorus, photosynthetic bacteria, EM bacteria and micro-mango algae into a fermentation culture medium respectively, culturing for 48 hours to obtain bacteria/algae liquid, concentrating and drying the bacteria/algae liquid obtained by fermentation.
(2) Compounding of microbial agents: the dried bacteria/algae are mixed and compounded according to the mass parts of 8 parts of bacillus amyloliquefaciens, 6 parts of paenibacillus piri, 3 parts of bdellovibrio bacteriovorus, 3 parts of photosynthetic bacteria, 6 parts of EM bacteria, 6 parts of micro-mango algae, 1 part of carbamide peroxide, 5 parts of chitosan, 4 parts of humic acid and 100 parts of diatomite, so as to obtain the microbial composite microbial agent.
Comparative example 1
(1) Preparing bacterial liquid: inoculating activated bacillus amyloliquefaciens, bdellovibrio bacteriovorus, photosynthetic bacteria, EM bacteria and micro-mango algae into a fermentation culture medium respectively, culturing for 48 hours to obtain bacteria/algae liquid, and concentrating and drying the bacteria/algae liquid obtained by fermentation.
(2) Compounding of microbial agents: the dried bacteria/algae are mixed and compounded according to the mass parts of 10 parts of bacillus amyloliquefaciens, 4 parts of bdellovibrio bacteriovorus, 3 parts of photosynthetic bacteria, 5 parts of EM bacteria, 5 parts of micro-mango algae, 1 part of carbamide peroxide, 3 parts of chitosan, 5 parts of humic acid and 100 parts of diatomite, so as to obtain the microbial composite microbial agent.
Comparative example 2
(1) Preparing bacterial liquid: inoculating activated bacillus amyloliquefaciens, paenibacillus piri, photosynthetic bacteria, EM bacteria and micro-mango algae into a fermentation culture medium respectively, culturing for 48 hours to obtain bacteria/algae liquid, concentrating and drying the bacteria/algae liquid obtained by fermentation.
(2) Compounding of microbial agents: the dried bacteria/algae are mixed and compounded according to the mass parts of 10 parts of bacillus amyloliquefaciens, 8 parts of paenibacillus piri, 3 parts of photosynthetic bacteria, 5 parts of EM bacteria, 5 parts of micro-mango algae, 1 part of carbamide peroxide, 3 parts of chitosan, 5 parts of humic acid and 100 parts of diatomite, so as to obtain the microbial compound microbial inoculum.
Comparative example 3
(1) Preparing bacterial liquid: inoculating activated bacillus amyloliquefaciens, paenibacillus piri, bdellovibrio bacteriovorus, photosynthetic bacteria and EM bacteria into a fermentation culture medium respectively, culturing for 48 hours to obtain bacterial liquid, concentrating and drying the bacterial liquid obtained by fermentation.
(2) Compounding of microbial agents: and mixing and compounding 10 parts of dried thalli, 8 parts of paenibacillus piri, 4 parts of bdellovibrio bacteriovorus, 3 parts of photosynthetic bacteria, 5 parts of EM bacteria, 1 part of carbamide peroxide, 3 parts of chitosan, 5 parts of humic acid and 100 parts of diatomite according to the parts by weight to obtain the microbial composite microbial agent.
Comparative example 4
(1) Preparing bacterial liquid: inoculating activated bacillus subtilis, paenibacillus piri, bdellovibrio bacteriovorus, photosynthetic bacteria, EM bacteria and micro-mango algae into a fermentation medium respectively, culturing for 48 hours to obtain bacteria/algae liquid, concentrating and drying the bacteria/algae liquid obtained by fermentation.
(2) Compounding of microbial agents: the dried bacteria/algae are mixed and compounded according to the mass parts of 10 parts of bacillus subtilis, 8 parts of paenibacillus piri, 4 parts of bdellovibrio bacteriovorus, 3 parts of photosynthetic bacteria, 5 parts of EM bacteria, 5 parts of micro-mango algae, 1 part of carbamide peroxide, 3 parts of chitosan, 5 parts of humic acid and 100 parts of diatomite, so as to obtain the microbial composite microbial agent.
Comparative example 5
(1) Preparing bacterial liquid: inoculating activated bacillus amyloliquefaciens, paenibacillus piri, bdellovibrio bacteriovorus, photosynthetic bacteria, EM bacteria and chlorella respectively into a fermentation medium, culturing for 48 hours to obtain bacteria/algae liquid, concentrating and drying the bacteria/algae liquid obtained by fermentation.
(2) Compounding of microbial agents: the dried bacteria/algae are mixed and compounded according to the mass parts of 10 parts of bacillus amyloliquefaciens, 8 parts of paenibacillus piri, 4 parts of bdellovibrio bacteriovorus, 3 parts of photosynthetic bacteria, 5 parts of EM bacteria, 5 parts of chlorella, 1 part of carbamide peroxide, 3 parts of chitosan, 5 parts of humic acid and 100 parts of diatomite, so as to obtain the microbial composite bacterial agent.
Experimental method and results:
1. the microbial composite microbial agents prepared in the examples 1-3 and the comparative examples 1-5 are respectively added into a sewage water sample according to the amount of 1kg/10000L of water, the total nitrogen, the total phosphorus, the ammonia nitrogen, the COD index and the water transparency in the water sample are measured after 3 days, and the water transparency is measured by adopting a Seiki plate method. Taking a water sample without any microbial inoculum as a blank control group. The experimental results are shown in tables 1-5:
TABLE 1 removal of ammonia nitrogen from Water
TABLE 2 removal of Total Nitrogen from Water
TABLE 3 removal of Total phosphorus from Water
TABLE 4 variation of COD in Water
TABLE 5 variation of Water transparency
The results show that the microbial composite microbial agent can reduce ammonia nitrogen, total phosphorus and COD index removal rate in sewage to 60% in a short time, particularly the total nitrogen removal rate to 94.43%, and can greatly improve the transparency of water body from 5.50cm to 27.00cm. The bacteria and algae in the microbial composite microbial inoculum have strong combination and synergistic capability, do not repel each other, quickly form dominant bacterial colonies in the water environment, degrade nutrient substances in the water and eliminate odor. Any one of bacteria/algae is removed or replaced, and the purifying effect of the bacteria/algae on the water body is greatly reduced.
2. The microbial composite microbial agent prepared in the example 2 is added into a sewage water sample according to the amount of 1kg/10000L of water, and the total nitrogen, total phosphorus, ammonia nitrogen, COD index and water transparency in the water sample are respectively measured after 0, 1, 2, 3 and 6 months, and the water transparency is measured by a Sai-disc method, and the results are shown in Table 6.
TABLE 6 Water quality variation at different treatment times for wastewater treatment
The microbial composite microbial inoculum can not only rapidly purify water in a short time, but also continuously play a role in the water environment, and long-term maintain the purification effect, and the purification time is as long as 6 months. The microbial composite microbial agent is characterized in that bacteria and algae in the microbial composite microbial agent form dominant bacteria/algae groups in a water body, so that a microbial ecological structure in the water body is changed, a better microbial environment is formed, the water body can be purified for a long time, the symptoms and root causes are treated, and the technical problems that the water body purifying agent in the prior art is short in purifying time and needs to be repeatedly added are solved.
The foregoing description is only a preferred embodiment of the present invention, and is not intended to limit the present invention in any way, and any simple modification, equivalent material change and modification made to the above examples according to the technical matter of the present invention still fall within the scope of the technical solution of the present invention.

Claims (13)

1. A microbial composite microbial agent for improving water quality is characterized in that: the microbial composite microbial agent comprises the following raw materials in parts by weight: bacillus amyloliquefaciens @Bacillus amyloliquefaciens) 6-10 parts of Paenibacillus piriPaenibacillus peoriae) 6-8 parts of bdellovibrio bacteriophagic bacteriaBdellovibrio bacteriouorus) 2-4 parts of photosynthetic bacteria, 3-5 parts of EM bacteria, 4-6 parts of micro-miscanthusMicractinium pusillum) 2-6 parts.
2. The microbial composite agent for water quality improvement according to claim 1, wherein: the microbial composite microbial agent comprises the following raw materials in parts by weight: bacillus amyloliquefaciens（Bacillus amyloliquefaciens) 10 parts of Paenibacillus piriPaenibacillus peoriae) 8 parts of bdellovibrio bacteriophagic bacteriaBdellovibrio bacteriouorus) 4 parts of photosynthetic bacteria, 3 parts of EM bacteria, 5 parts of micro-mango algaeMicractinium pusillum) 5 parts.
3. The microbial composite agent for water quality improvement according to claim 1 or 2, characterized in that: the microbial composite inoculant also comprises the following components: 1-2 parts of carbamide peroxide, 1-5 parts of chitosan and 4-6 parts of humic acid.
4. The microbial composite agent for water quality improvement according to claim 1 or 2, characterized in that: the microbial composite inoculant also comprises the following components: 1 part of carbamide peroxide, 3 parts of chitosan and 5 parts of humic acid.
5. The microbial composite agent for water quality improvement according to claim 1 or 2, characterized in that: the microbial composite bacterial agent also comprises 100-120 parts of microbial carrier, wherein the microbial carrier is diatomite or wood dust.
6. The method for preparing the microbial composite agent for water quality improvement according to any one of claims 1 to 5, which is characterized in that: the method specifically comprises the following steps:
(1) Preparing bacterial liquid: inoculating activated bacillus amyloliquefaciens, paenibacillus piri, bdellovibrio bacteriovorus, photosynthetic bacteria, EM bacteria and micro-mango algae into a fermentation medium respectively, culturing for 24-48 hours to obtain bacteria/algae liquid, concentrating and drying the bacteria/algae liquid obtained by fermentation;
(2) Compounding of microbial agents: 6-10 parts of bacillus amyloliquefaciens, 6-8 parts of paenibacillus piri, 2-4 parts of bdellovibrio bacteriovorus, 3-5 parts of photosynthetic bacteria, 4-6 parts of EM bacteria and 2-6 parts of micro-mango algae are mixed and compounded according to the parts by weight.
7. The method for preparing the microbial composite agent for water quality improvement according to claim 6, which is characterized in that: in the step (1), the fermentation medium of the bacillus amyloliquefaciens, the paenibacillus piri and the EM bacteria comprises the following components: 67.0g/L of soluble corn starch, 41.6g/L of peanut cake soaked powder, 10g/L, K of peptone 2 HPO 4 ·3H 2 O9.2 g/L and MgSO 4 ·7H 2 O 1.5g/L。
8. The method for preparing the microbial composite agent for water quality improvement according to claim 6, which is characterized in that: in the step (1), the fermentation medium of the bacteriophagic bdellovibrio comprises the following components: beef extract 3g/L, tryptone 10g/L, yeast extract 5g/L, sodium chloride 10g/L.
9. The method for preparing the microbial composite agent for water quality improvement according to claim 6, which is characterized in that: in the step (1), the fermentation medium of the photosynthetic bacteria comprises the following components: NH (NH) 4 Cl 1g/L，CH 3 COONa 3.5g/L，MgCl 2 0.1g/L，CaCl 2 0.1g/L，KH 2 PO 4 0.6g/L，K 2 HPO 4 0.4g/L, and yeast extract 0.1g/L.
10. The method for preparing the microbial composite agent for water quality improvement according to claim 6, which is characterized in that: in the step (1), the fermentation medium of the micro-mango algae is a BG11 medium.
11. The method for preparing the microbial composite agent for water quality improvement according to claim 6, which is characterized in that: in the microbial agent compounding of the step (2), 1-2 parts of carbamide peroxide, 1-5 parts of chitosan, 4-6 parts of humic acid and 100-120 parts of microbial carrier can be added.
12. A method for improving water quality, which is characterized in that: applying the microbial composite microbial agent for water quality improvement according to any one of claims 1 to 5 or the microbial composite microbial agent prepared according to the method according to any one of claims 6 to 11 to sewage.
13. The use of the microbial composite agent for water quality improvement according to any one of claims 1 to 5 or the microbial composite agent prepared according to the method according to any one of claims 6 to 11 in purifying water.
CN202310800398.6A 2023-07-03 2023-07-03 Microbial composite microbial agent for water quality improvement, preparation method and application thereof Pending CN116515719A (en)

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