CN102992493B - A kind of improved combination microorganism renovation agent for cultivation water environment regulation and control - Google Patents

A kind of improved combination microorganism renovation agent for cultivation water environment regulation and control Download PDF

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Publication number
CN102992493B
CN102992493B CN201210401791.XA CN201210401791A CN102992493B CN 102992493 B CN102992493 B CN 102992493B CN 201210401791 A CN201210401791 A CN 201210401791A CN 102992493 B CN102992493 B CN 102992493B
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microorganism
renovation agent
surfactant
water
bacteria
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CN102992493A (en
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李学军
张大宏
黄辉
乔伟
陈�胜
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Beijing Sheng Da Da Biotechnology Co., Ltd.
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Beijing Sheng Da Da Biotechnology Co Ltd
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Abstract

The present invention relates to a kind of improved combination microorganism renovation agents for cultivation water environment regulation and control, belong to the scope of microorganism formulation.The renovation agent is made of surfactant and multiple-microorganism, and it is 5~15% that surfactant, which accounts for renovation agent mass percent, and surfactant includes palm quaternary ammonium salt, Magnesium dilaurate, Shuangzi base coco betaine, they add diluent to mix in 1: 1: 1 ratio;Mass percent of each microorganism in microbial total quality be:Bacillus subtilis 20%~40%, lactic acid bacteria 20~40%, Candida 10%~30%, denitrifying bacteria 5~10%, photosynthetic bacteria 5~10%.Above-mentioned renovation agent assembles by a certain percentage, ensure that complex microorganism is uniformly distributed in cultivation water environment, dissolved oxygen in water is set to increase, dominant microflora is quickly formed in water body, generate high-activity bio-enzyme, the decomposition for accelerating residual bait and animal wastes, inhibits the growth of harmful bacteria, it is ensured that the production of green aquatic product.

Description

A kind of improved combination microorganism renovation agent for cultivation water environment regulation and control
Technical field
The present invention relates to a kind of improved combination microorganism renovation agents for cultivation water environment regulation and control, belong to microorganism The scope of preparation.
Background technology
Due to breeding water body residual bait, animal void excreta the hydrophobicity of middle organic pollution and organic matter may be by Strong adsorption is on suspended particulate surface or enters bed mud soil aperture, makes the reduction of aqueous solution organic concentration, cause microorganism and The isolation of organic pollution.Since microorganism cannot utilize the organic matter of extremely low concentration, the microbial degradation of organic matter is caused to stop Only or slow down.Therefore, the conclusive influence factor of the biological prosthetic middle degradation of organic substances of breeding water body is the low water-soluble of organic matter Property, this makes its bioavailability be restricted.But the dissolubility of organic matter can be increased by applying surfactant, changed The bioavailability of kind hardly degraded organic substance, promotes organic matter degradation.
Biosurfactant enhances the emulsification degree of organic matter, promotes its distribution in water phase, that is, forms The so-called molten phenomenon of vacation, to be conducive to microbial degradation organic matter, meanwhile, it is thin that biosurfactant also changes microorganism The surface nature of born of the same parents so that microorganism is easy to and hydrophobic organic compound is close or microorganism is to the Contact-sorption on solid dielectric surface Ability enhances, and to be finally reached the ability that enhancing microorganism panning utilizes organic matter, while biosurfactant increases oxygen Saturation degree in water body.
Microorganism formulation about breeding water body, it has been disclosed that patent have a kind of " lactic acid bacteria for cultivation water regulation The production method of probiotics ", " a kind of production method of bdellovibrio bacteriovorus ecological preparation ", a kind of " water environment microorganism remediation Agent " and " a kind of probiotics of aquaculture ", " a kind of compound micro-ecological preparation dosage form of purifying aquatic water and its preparation Technique " etc., but used using surfactant and microorganism formulation proportioning, currently without report and application, so applicant exists On the basis of meticulous experimental study, it is proposed that this item patent application.
Invention content
At present surfactant in aquaculture mostly as sterilization and disinfectant, some products for substrate improvement and Water oxygenation, the present invention combine surfactant and microorganism formulation by long term test and strain selection and mutagenesis improvement, It is combined into a kind of high-effective microorganism renovation agent improving breeding water body environment, it is micro- advantage to be quickly formed after in breeding water body Biotic population improves the ability of organic matter in degradation water body, breeding water body water quality is made to improve quickly, promotes cultivated animals growth, Cultivation survival rate is improved, fanning economics are increased.
The purpose of the present invention is what is be achieved through the following technical solutions:
For the improved combination microorganism renovation agent of cultivation water environment regulation and control, group is divided into surfactant and a variety of micro- Biology, wherein it is 5~15% that surfactant, which accounts for renovation agent mass percent, and surfactant includes palm quaternary ammonium salt, bay Sour magnesium, Shuangzi base coco betaine, they add diluent to mix in 1: 1: 1 ratio;Each microorganism is in microbial total quality Mass percent be:Bacillus subtilis 20%~40%, lactic acid bacteria 20~40%, Candida 10%~30%, anti-nitre Change bacterium 5~10%, photosynthetic bacteria 5~10%.
The advantages and positive effects of the present invention are:
(1) surfactant is added in the present invention in formula, can significantly improve Dissolved Oxygen in Water, it is micro- to quickly form advantage Biotic population improves the ability of organic matter in degradation water body, breeding water body water quality is made to improve quickly.
(2) bacillus subtilis, lactic acid bacteria, Candida, denitrifying bacteria, photosynthetic thin is added in the present invention in formula Bacterium can be obviously improved cultivation water;Bacillus subtilis can generate biosurfactant, improve culture efficiency, both have and add The effect of strong fish body nutrition, and the immunity of body can be improved, to be obviously promoted fish body growth, improve cultivation survival rate. Research shows that 3~5% growth rate can be improved, cultivation survival rate improves 7~10%.
Specific implementation mode
It is further illustrated the present invention below in conjunction with specific implementation method.
A kind of improved combination microorganism renovation agent for cultivation water environment regulation and control belongs to the scope of microorganism formulation. The renovation agent is made of surfactant and multiple-microorganism, wherein surfactant account for renovation agent mass percent be 5~ 15%, surfactant includes palm quaternary ammonium salt, Magnesium dilaurate, Shuangzi base coco betaine, they add dilution in 1: 1: 1 ratio Agent mixes;Mass percent of each microorganism in microbial total quality be:Bacillus subtilis 20%~40%, lactic acid bacteria 20~40%, Candida 10%~30%, denitrifying bacteria 5~10%, photosynthetic bacteria 5~10%.The renovation agent of the present invention It assembling by a certain percentage, plays the synergistic function of each component, it is ensured that complex microorganism is uniformly distributed in cultivation water environment, Improve the ability of purifying aquaculture water quality.Above-mentioned renovation agent is adaptable strong, and putting into water can fully be distributed in upper and lower water layer, make Dissolved oxygen in water increases, and is beneficial to quickly form dominant microflora in water body, and can generate high-activity bio-enzyme, accelerates residual bait and animal The decomposition of excrement, while antibiotic sample substance can be generated, inhibit the growth of harmful bacteria, reduces the generation of disease, it is ensured that Green Water The production of product.
Mentioned microorganism renovation agent specifically matches mode:
(1) it is 5% that surfactant, which accounts for renovation agent mass percent, quality of the various microorganisms in microbial total quality Percentage is:Bacillus subtilis 30%, lactic acid bacteria 30%, Candida 10%~30%, denitrifying bacteria 5~10%, light Close bacterium 5~10%.
(2) it is 10% that surfactant, which accounts for renovation agent mass percent, matter of the various microorganisms in microbial total quality Measuring percentage is:Bacillus subtilis 30%, lactic acid bacteria 40%, Candida 10%~20%, denitrifying bacteria 5~10%, Photosynthetic bacteria 5~10%.
(3) it is 15% that surfactant, which accounts for renovation agent mass percent, matter of the various microorganisms in microbial total quality Measuring percentage is:Bacillus subtilis 40%, lactic acid bacteria 30%, Candida 15%, denitrifying bacteria 5~10% are photosynthetic thin Bacterium 5~10%.
Experiments have shown that before using the renovation agent, the oxygen dissolving value of bottom of pond water body is 3.82~4.96mg/L.It is added in pool water within The renovation agent, a concentration of 1mg/L, water body dissolved oxygen, which increases to 5.38~6.24mg/L only, to be needed 10 minutes, and the duration was up to 2 days.
In above-mentioned technical proposal, which has wider accommodation to environmental condition, can be in natural lighting or people Growth in work illumination, ventilation or stuffiness, 5.5~8.5 ranges of pH.Prove the renovation agent to water by aquaculture pond inoculation test NO in body2 -- N, NH4 +- N, NH3- N has significant removal to act on, wherein with to NO2 -The clean-up effect of-N is the most prominent, from It is inoculated with 3d and is held in 55~63% or so to 15d removal rates;To NH4 +The removal rate of-N is more than 15 from 6d to 12d is inoculated with ~20%;To NH3The removal rate of-N is from 3d to 9d more than 38~45%;It is more than from inoculation 3d to 15d to the removal rate of COD 15~17.9%.
It is emphasized that:To those skilled in the art, it can be improved or become according to following explanation It changes, and all these modifications and variations should all belong to the protection domain of appended claims of the present invention.

Claims (1)

1. a kind of improved combination microorganism renovation agent for cultivation water environment regulation and control, it is characterised in that:Above-mentioned renovation agent group It is divided into surfactant and multiple-microorganism, wherein it is 5~15% that surfactant, which accounts for renovation agent mass percent, and surface is lived Property agent includes palm quaternary ammonium salt, Magnesium dilaurate, Shuangzi base coco betaine, they add diluent to mix in 1: 1: 1 ratio;Each Mass percent of the microorganism in microbial total quality be:Bacillus subtilis 20%~40%, lactic acid bacteria 20~40% are false Silk yeast 10%~30%, denitrifying bacteria 5~10%, photosynthetic bacteria 5~10%.
CN201210401791.XA 2012-10-22 2012-10-22 A kind of improved combination microorganism renovation agent for cultivation water environment regulation and control Active CN102992493B (en)

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Families Citing this family (9)

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CN103232097B (en) * 2013-04-15 2014-04-09 中国水产科学研究院淡水渔业研究中心 Composite preparation for increasing dissolved oxygen content in pond culture water body
CN103667152A (en) * 2013-12-19 2014-03-26 云南晋企生物科技有限公司 Universal microorganism bacterial agent
CN103933894B (en) * 2014-03-12 2015-12-02 华南理工大学 A kind of regulate and control the method for breeding water body environment and complexed surfactant
CN105062915A (en) * 2015-07-23 2015-11-18 湖南康易达绿茵科技有限公司 Microecological preparation for degrading N and P (nitrogen and phosphorus)
CN105540853A (en) * 2015-12-18 2016-05-04 刘高志 Bioremediation method for copper-bearing sewage
CN105481096A (en) * 2015-12-18 2016-04-13 刘高志 Method for biological remediation of copper-containing sewage
CN107381831A (en) * 2017-07-25 2017-11-24 明光市永言水产(集团)有限公司 A kind of aquaculture system modifying agent
CN108358269A (en) * 2018-02-06 2018-08-03 浦江县酉泽水产科技有限公司 The preparation method of tilapia water quality improving agent
CN109305744A (en) * 2018-11-27 2019-02-05 上海城市水资源开发利用国家工程中心有限公司 A kind of black and odorous water biological renovation method and system

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CN101147529B (en) * 2007-10-31 2010-07-28 无锡中顺生物技术有限公司 Microecological preparation for aquaculture and its preparation method
CN100588624C (en) * 2008-05-08 2010-02-10 储卫华 Microorganism renovation agent of water environment and preparation method thereof
CN101353636B (en) * 2008-09-11 2010-09-01 中国水产科学研究院南海水产研究所 Production method of lactobacillus micro-ecological preparation for cultivation water regulation
US8784788B2 (en) * 2011-03-29 2014-07-22 Environmentally Sensitive Solutions, Inc. Gel carrier for releasing active ingredients

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