CN102992493B - A kind of improved combination microorganism renovation agent for cultivation water environment regulation and control - Google Patents
A kind of improved combination microorganism renovation agent for cultivation water environment regulation and control Download PDFInfo
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- CN102992493B CN102992493B CN201210401791.XA CN201210401791A CN102992493B CN 102992493 B CN102992493 B CN 102992493B CN 201210401791 A CN201210401791 A CN 201210401791A CN 102992493 B CN102992493 B CN 102992493B
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- microorganism
- renovation agent
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- bacteria
- water environment
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- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 title claims abstract description 38
- 244000005700 microbiome Species 0.000 title claims abstract description 31
- 239000003795 chemical substances by application Substances 0.000 title claims abstract description 28
- 238000009418 renovation Methods 0.000 title claims abstract description 25
- 241000894006 Bacteria Species 0.000 claims abstract description 27
- 239000004094 surface-active agent Substances 0.000 claims abstract description 19
- JVTAAEKCZFNVCJ-UHFFFAOYSA-N lactic acid Chemical compound CC(O)C(O)=O JVTAAEKCZFNVCJ-UHFFFAOYSA-N 0.000 claims abstract description 18
- 244000063299 Bacillus subtilis Species 0.000 claims abstract description 9
- 235000014469 Bacillus subtilis Nutrition 0.000 claims abstract description 9
- 235000014655 lactic acid Nutrition 0.000 claims abstract description 9
- 239000004310 lactic acid Substances 0.000 claims abstract description 9
- 230000000813 microbial effect Effects 0.000 claims abstract description 9
- 230000000243 photosynthetic effect Effects 0.000 claims abstract description 7
- 150000003242 quaternary ammonium salts Chemical class 0.000 claims abstract description 4
- FSYKKLYZXJSNPZ-UHFFFAOYSA-N sarcosine Chemical compound C[NH2+]CC([O-])=O FSYKKLYZXJSNPZ-UHFFFAOYSA-N 0.000 claims abstract description 4
- 239000003085 diluting agent Substances 0.000 claims abstract description 3
- BJZBHTNKDCBDNQ-UHFFFAOYSA-L magnesium;dodecanoate Chemical compound [Mg+2].CCCCCCCCCCCC([O-])=O.CCCCCCCCCCCC([O-])=O BJZBHTNKDCBDNQ-UHFFFAOYSA-L 0.000 claims abstract description 3
- 240000004808 Saccharomyces cerevisiae Species 0.000 claims 1
- 241000222120 Candida <Saccharomycetales> Species 0.000 abstract description 7
- QVGXLLKOCUKJST-UHFFFAOYSA-N atomic oxygen Chemical compound [O] QVGXLLKOCUKJST-UHFFFAOYSA-N 0.000 abstract description 6
- 229910052760 oxygen Inorganic materials 0.000 abstract description 6
- 239000001301 oxygen Substances 0.000 abstract description 6
- 238000009472 formulation Methods 0.000 abstract description 5
- 230000012010 growth Effects 0.000 abstract description 5
- 239000000203 mixture Substances 0.000 abstract description 5
- 230000000694 effects Effects 0.000 abstract description 4
- 238000004519 manufacturing process Methods 0.000 abstract description 4
- 238000000354 decomposition reaction Methods 0.000 abstract description 2
- 244000005706 microflora Species 0.000 abstract description 2
- 239000010828 animal waste Substances 0.000 abstract 1
- 239000005416 organic matter Substances 0.000 description 11
- 238000009395 breeding Methods 0.000 description 7
- 230000001488 breeding effect Effects 0.000 description 7
- 230000015556 catabolic process Effects 0.000 description 6
- 238000006731 degradation reaction Methods 0.000 description 6
- 238000009360 aquaculture Methods 0.000 description 4
- 244000144974 aquaculture Species 0.000 description 4
- 239000003876 biosurfactant Substances 0.000 description 4
- 238000002360 preparation method Methods 0.000 description 4
- 241001465754 Metazoa Species 0.000 description 3
- 230000008901 benefit Effects 0.000 description 3
- 239000000126 substance Substances 0.000 description 3
- 230000004083 survival effect Effects 0.000 description 3
- 241000251468 Actinopterygii Species 0.000 description 2
- 210000000476 body water Anatomy 0.000 description 2
- 230000006872 improvement Effects 0.000 description 2
- 238000011081 inoculation Methods 0.000 description 2
- 238000000034 method Methods 0.000 description 2
- 239000006041 probiotic Substances 0.000 description 2
- 235000018291 probiotics Nutrition 0.000 description 2
- 238000001179 sorption measurement Methods 0.000 description 2
- 238000012360 testing method Methods 0.000 description 2
- 241000604931 Bdellovibrio bacteriovorus Species 0.000 description 1
- FYYHWMGAXLPEAU-UHFFFAOYSA-N Magnesium Chemical compound [Mg] FYYHWMGAXLPEAU-UHFFFAOYSA-N 0.000 description 1
- 230000004308 accommodation Effects 0.000 description 1
- 239000007864 aqueous solution Substances 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 230000003115 biocidal effect Effects 0.000 description 1
- 230000008859 change Effects 0.000 description 1
- 150000001875 compounds Chemical class 0.000 description 1
- 239000000645 desinfectant Substances 0.000 description 1
- 238000010790 dilution Methods 0.000 description 1
- 239000012895 dilution Substances 0.000 description 1
- 201000010099 disease Diseases 0.000 description 1
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 description 1
- 238000009826 distribution Methods 0.000 description 1
- 239000002552 dosage form Substances 0.000 description 1
- 238000004945 emulsification Methods 0.000 description 1
- 238000005516 engineering process Methods 0.000 description 1
- 230000002708 enhancing effect Effects 0.000 description 1
- 230000007613 environmental effect Effects 0.000 description 1
- 238000002474 experimental method Methods 0.000 description 1
- 210000003608 fece Anatomy 0.000 description 1
- 230000002209 hydrophobic effect Effects 0.000 description 1
- 238000005286 illumination Methods 0.000 description 1
- 230000036039 immunity Effects 0.000 description 1
- 238000002955 isolation Methods 0.000 description 1
- 230000007774 longterm Effects 0.000 description 1
- 229910052749 magnesium Inorganic materials 0.000 description 1
- 239000011777 magnesium Substances 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 230000021332 multicellular organism growth Effects 0.000 description 1
- 238000002703 mutagenesis Methods 0.000 description 1
- 231100000350 mutagenesis Toxicity 0.000 description 1
- 235000016709 nutrition Nutrition 0.000 description 1
- 230000035764 nutrition Effects 0.000 description 1
- 150000002894 organic compounds Chemical class 0.000 description 1
- 238000006213 oxygenation reaction Methods 0.000 description 1
- 238000004091 panning Methods 0.000 description 1
- 230000008092 positive effect Effects 0.000 description 1
- 230000009467 reduction Effects 0.000 description 1
- 238000005067 remediation Methods 0.000 description 1
- 238000011160 research Methods 0.000 description 1
- 239000002689 soil Substances 0.000 description 1
- 239000007787 solid Substances 0.000 description 1
- 239000000243 solution Substances 0.000 description 1
- 230000001954 sterilising effect Effects 0.000 description 1
- 238000004659 sterilization and disinfection Methods 0.000 description 1
- 239000000758 substrate Substances 0.000 description 1
- 230000002195 synergetic effect Effects 0.000 description 1
- 238000009423 ventilation Methods 0.000 description 1
- 239000011800 void material Substances 0.000 description 1
Landscapes
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Fodder In General (AREA)
Abstract
The present invention relates to a kind of improved combination microorganism renovation agents for cultivation water environment regulation and control, belong to the scope of microorganism formulation.The renovation agent is made of surfactant and multiple-microorganism, and it is 5~15% that surfactant, which accounts for renovation agent mass percent, and surfactant includes palm quaternary ammonium salt, Magnesium dilaurate, Shuangzi base coco betaine, they add diluent to mix in 1: 1: 1 ratio；Mass percent of each microorganism in microbial total quality be：Bacillus subtilis 20%~40%, lactic acid bacteria 20~40%, Candida 10%~30%, denitrifying bacteria 5~10%, photosynthetic bacteria 5~10%.Above-mentioned renovation agent assembles by a certain percentage, ensure that complex microorganism is uniformly distributed in cultivation water environment, dissolved oxygen in water is set to increase, dominant microflora is quickly formed in water body, generate high-activity bio-enzyme, the decomposition for accelerating residual bait and animal wastes, inhibits the growth of harmful bacteria, it is ensured that the production of green aquatic product.
Description
Technical field
The present invention relates to a kind of improved combination microorganism renovation agents for cultivation water environment regulation and control, belong to microorganism
The scope of preparation.
Background technology
Due to breeding water body residual bait, animal void excreta the hydrophobicity of middle organic pollution and organic matter may be by
Strong adsorption is on suspended particulate surface or enters bed mud soil aperture, makes the reduction of aqueous solution organic concentration, cause microorganism and
The isolation of organic pollution.Since microorganism cannot utilize the organic matter of extremely low concentration, the microbial degradation of organic matter is caused to stop
Only or slow down.Therefore, the conclusive influence factor of the biological prosthetic middle degradation of organic substances of breeding water body is the low water-soluble of organic matter
Property, this makes its bioavailability be restricted.But the dissolubility of organic matter can be increased by applying surfactant, changed
The bioavailability of kind hardly degraded organic substance, promotes organic matter degradation.
Biosurfactant enhances the emulsification degree of organic matter, promotes its distribution in water phase, that is, forms
The so-called molten phenomenon of vacation, to be conducive to microbial degradation organic matter, meanwhile, it is thin that biosurfactant also changes microorganism
The surface nature of born of the same parents so that microorganism is easy to and hydrophobic organic compound is close or microorganism is to the Contact-sorption on solid dielectric surface
Ability enhances, and to be finally reached the ability that enhancing microorganism panning utilizes organic matter, while biosurfactant increases oxygen
Saturation degree in water body.
Microorganism formulation about breeding water body, it has been disclosed that patent have a kind of " lactic acid bacteria for cultivation water regulation
The production method of probiotics ", " a kind of production method of bdellovibrio bacteriovorus ecological preparation ", a kind of " water environment microorganism remediation
Agent " and " a kind of probiotics of aquaculture ", " a kind of compound micro-ecological preparation dosage form of purifying aquatic water and its preparation
Technique " etc., but used using surfactant and microorganism formulation proportioning, currently without report and application, so applicant exists
On the basis of meticulous experimental study, it is proposed that this item patent application.
Invention content
At present surfactant in aquaculture mostly as sterilization and disinfectant, some products for substrate improvement and
Water oxygenation, the present invention combine surfactant and microorganism formulation by long term test and strain selection and mutagenesis improvement,
It is combined into a kind of high-effective microorganism renovation agent improving breeding water body environment, it is micro- advantage to be quickly formed after in breeding water body
Biotic population improves the ability of organic matter in degradation water body, breeding water body water quality is made to improve quickly, promotes cultivated animals growth,
Cultivation survival rate is improved, fanning economics are increased.
The purpose of the present invention is what is be achieved through the following technical solutions：
For the improved combination microorganism renovation agent of cultivation water environment regulation and control, group is divided into surfactant and a variety of micro-
Biology, wherein it is 5~15% that surfactant, which accounts for renovation agent mass percent, and surfactant includes palm quaternary ammonium salt, bay
Sour magnesium, Shuangzi base coco betaine, they add diluent to mix in 1: 1: 1 ratio；Each microorganism is in microbial total quality
Mass percent be：Bacillus subtilis 20%~40%, lactic acid bacteria 20~40%, Candida 10%~30%, anti-nitre
Change bacterium 5~10%, photosynthetic bacteria 5~10%.
The advantages and positive effects of the present invention are：
(1) surfactant is added in the present invention in formula, can significantly improve Dissolved Oxygen in Water, it is micro- to quickly form advantage
Biotic population improves the ability of organic matter in degradation water body, breeding water body water quality is made to improve quickly.
(2) bacillus subtilis, lactic acid bacteria, Candida, denitrifying bacteria, photosynthetic thin is added in the present invention in formula
Bacterium can be obviously improved cultivation water；Bacillus subtilis can generate biosurfactant, improve culture efficiency, both have and add
The effect of strong fish body nutrition, and the immunity of body can be improved, to be obviously promoted fish body growth, improve cultivation survival rate.
Research shows that 3~5% growth rate can be improved, cultivation survival rate improves 7~10%.
Specific implementation mode
It is further illustrated the present invention below in conjunction with specific implementation method.
A kind of improved combination microorganism renovation agent for cultivation water environment regulation and control belongs to the scope of microorganism formulation.
The renovation agent is made of surfactant and multiple-microorganism, wherein surfactant account for renovation agent mass percent be 5~
15%, surfactant includes palm quaternary ammonium salt, Magnesium dilaurate, Shuangzi base coco betaine, they add dilution in 1: 1: 1 ratio
Agent mixes；Mass percent of each microorganism in microbial total quality be：Bacillus subtilis 20%~40%, lactic acid bacteria
20~40%, Candida 10%~30%, denitrifying bacteria 5~10%, photosynthetic bacteria 5~10%.The renovation agent of the present invention
It assembling by a certain percentage, plays the synergistic function of each component, it is ensured that complex microorganism is uniformly distributed in cultivation water environment,
Improve the ability of purifying aquaculture water quality.Above-mentioned renovation agent is adaptable strong, and putting into water can fully be distributed in upper and lower water layer, make
Dissolved oxygen in water increases, and is beneficial to quickly form dominant microflora in water body, and can generate high-activity bio-enzyme, accelerates residual bait and animal
The decomposition of excrement, while antibiotic sample substance can be generated, inhibit the growth of harmful bacteria, reduces the generation of disease, it is ensured that Green Water
The production of product.
Mentioned microorganism renovation agent specifically matches mode：
(1) it is 5% that surfactant, which accounts for renovation agent mass percent, quality of the various microorganisms in microbial total quality
Percentage is：Bacillus subtilis 30%, lactic acid bacteria 30%, Candida 10%~30%, denitrifying bacteria 5~10%, light
Close bacterium 5~10%.
(2) it is 10% that surfactant, which accounts for renovation agent mass percent, matter of the various microorganisms in microbial total quality
Measuring percentage is：Bacillus subtilis 30%, lactic acid bacteria 40%, Candida 10%~20%, denitrifying bacteria 5~10%,
Photosynthetic bacteria 5~10%.
(3) it is 15% that surfactant, which accounts for renovation agent mass percent, matter of the various microorganisms in microbial total quality
Measuring percentage is：Bacillus subtilis 40%, lactic acid bacteria 30%, Candida 15%, denitrifying bacteria 5~10% are photosynthetic thin
Bacterium 5~10%.
Experiments have shown that before using the renovation agent, the oxygen dissolving value of bottom of pond water body is 3.82~4.96mg/L.It is added in pool water within
The renovation agent, a concentration of 1mg/L, water body dissolved oxygen, which increases to 5.38~6.24mg/L only, to be needed 10 minutes, and the duration was up to 2 days.
In above-mentioned technical proposal, which has wider accommodation to environmental condition, can be in natural lighting or people
Growth in work illumination, ventilation or stuffiness, 5.5~8.5 ranges of pH.Prove the renovation agent to water by aquaculture pond inoculation test
NO in body2 -- N, NH4 +- N, NH3- N has significant removal to act on, wherein with to NO2 -The clean-up effect of-N is the most prominent, from
It is inoculated with 3d and is held in 55~63% or so to 15d removal rates；To NH4 +The removal rate of-N is more than 15 from 6d to 12d is inoculated with
~20%；To NH3The removal rate of-N is from 3d to 9d more than 38~45%；It is more than from inoculation 3d to 15d to the removal rate of COD
15~17.9%.
It is emphasized that：To those skilled in the art, it can be improved or become according to following explanation
It changes, and all these modifications and variations should all belong to the protection domain of appended claims of the present invention.
Claims (1)
1. a kind of improved combination microorganism renovation agent for cultivation water environment regulation and control, it is characterised in that：Above-mentioned renovation agent group
It is divided into surfactant and multiple-microorganism, wherein it is 5~15% that surfactant, which accounts for renovation agent mass percent, and surface is lived
Property agent includes palm quaternary ammonium salt, Magnesium dilaurate, Shuangzi base coco betaine, they add diluent to mix in 1: 1: 1 ratio；Each
Mass percent of the microorganism in microbial total quality be：Bacillus subtilis 20%~40%, lactic acid bacteria 20~40% are false
Silk yeast 10%~30%, denitrifying bacteria 5~10%, photosynthetic bacteria 5~10%.
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| CN201210401791.XA CN102992493B (en) | 2012-10-22 | 2012-10-22 | A kind of improved combination microorganism renovation agent for cultivation water environment regulation and control |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201210401791.XA CN102992493B (en) | 2012-10-22 | 2012-10-22 | A kind of improved combination microorganism renovation agent for cultivation water environment regulation and control |
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| Publication Number | Publication Date |
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| CN102992493A CN102992493A (en) | 2013-03-27 |
| CN102992493B true CN102992493B (en) | 2018-08-21 |
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Families Citing this family (9)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103232097B (en) * | 2013-04-15 | 2014-04-09 | 中国水产科学研究院淡水渔业研究中心 | Composite preparation for increasing dissolved oxygen content in pond culture water body |
| CN103667152A (en) * | 2013-12-19 | 2014-03-26 | 云南晋企生物科技有限公司 | Universal microorganism bacterial agent |
| CN103933894B (en) * | 2014-03-12 | 2015-12-02 | 华南理工大学 | A kind of regulate and control the method for breeding water body environment and complexed surfactant |
| CN105062915A (en) * | 2015-07-23 | 2015-11-18 | 湖南康易达绿茵科技有限公司 | Microecological preparation for degrading N and P (nitrogen and phosphorus) |
| CN105540853A (en) * | 2015-12-18 | 2016-05-04 | 刘高志 | Bioremediation method for copper-bearing sewage |
| CN105481096A (en) * | 2015-12-18 | 2016-04-13 | 刘高志 | Method for biological remediation of copper-containing sewage |
| CN107381831A (en) * | 2017-07-25 | 2017-11-24 | 明光市永言水产(集团)有限公司 | A kind of aquaculture system modifying agent |
| CN108358269A (en) * | 2018-02-06 | 2018-08-03 | 浦江县酉泽水产科技有限公司 | The preparation method of tilapia water quality improving agent |
| CN109305744A (en) * | 2018-11-27 | 2019-02-05 | 上海城市水资源开发利用国家工程中心有限公司 | A kind of black and odorous water biological renovation method and system |
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| Publication number | Priority date | Publication date | Assignee | Title |
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| CN101147529B (en) * | 2007-10-31 | 2010-07-28 | 无锡中顺生物技术有限公司 | Microecological preparation for aquaculture and its preparation method |
| CN100588624C (en) * | 2008-05-08 | 2010-02-10 | 储卫华 | Microorganism renovation agent of water environment and preparation method thereof |
| CN101353636B (en) * | 2008-09-11 | 2010-09-01 | 中国水产科学研究院南海水产研究所 | Production method of lactobacillus micro-ecological preparation for cultivation water regulation |
| US8784788B2 (en) * | 2011-03-29 | 2014-07-22 | Environmentally Sensitive Solutions, Inc. | Gel carrier for releasing active ingredients |
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Inventor after: Li Xuejun Inventor after: Zhang Dahong Inventor after: Huang Hui Inventor after: Qiao Wei Inventor after: Chen Sheng Inventor before: Zhang Dahong Inventor before: Xu Jian |
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Effective date of registration: 20160810 Address after: 100193 Beijing city Haidian District malianwa road Yicheng International Center Room 506 Applicant after: Beijing Sheng Da Da Biotechnology Co., Ltd. Address before: 100094 Beijing city northwest of Haidian District Wang Chunhui Garden District 12 building 3 unit 601 room Applicant before: Beijing Chiaher Animal Nutrition Technology Co., Ltd. |
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Denomination of invention: Improved combined microbe repairing agent for aquaculture environment regulation Effective date of registration: 20200424 Granted publication date: 20180821 Pledgee: Zhongguancun Beijing technology financing Company limited by guarantee Pledgor: BEIJING STROWIN BIOTECHNOLOGY Co.,Ltd. Registration number: Y2020990000356 |
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