CN106745821A - Sea-farming special microorganism water purification agent and preparation method thereof and application method - Google Patents

Sea-farming special microorganism water purification agent and preparation method thereof and application method Download PDF

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Publication number
CN106745821A
CN106745821A CN201710095159.XA CN201710095159A CN106745821A CN 106745821 A CN106745821 A CN 106745821A CN 201710095159 A CN201710095159 A CN 201710095159A CN 106745821 A CN106745821 A CN 106745821A
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water
sea
purification agent
zeolite
water purification
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徐永平
衡新
王熙涛
王丽丽
李晓宇
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Dalian University of Technology
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Dalian University of Technology
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    • CCHEMISTRY; METALLURGY
    • C02TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
    • C02FTREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
    • C02F3/00Biological treatment of water, waste water, or sewage
    • C02F3/34Biological treatment of water, waste water, or sewage characterised by the microorganisms used
    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01KANIMAL HUSBANDRY; CARE OF BIRDS, FISHES, INSECTS; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
    • A01K61/00Culture of aquatic animals
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/20Bacteria; Culture media therefor
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N11/00Carrier-bound or immobilised enzymes; Carrier-bound or immobilised microbial cells; Preparation thereof
    • C12N11/14Enzymes or microbial cells immobilised on or in an inorganic carrier
    • CCHEMISTRY; METALLURGY
    • C02TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
    • C02FTREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
    • C02F3/00Biological treatment of water, waste water, or sewage
    • C02F2003/001Biological treatment of water, waste water, or sewage using granular carriers or supports for the microorganisms
    • CCHEMISTRY; METALLURGY
    • C02TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
    • C02FTREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
    • C02F2101/00Nature of the contaminant
    • C02F2101/10Inorganic compounds
    • C02F2101/105Phosphorus compounds
    • CCHEMISTRY; METALLURGY
    • C02TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
    • C02FTREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
    • C02F2101/00Nature of the contaminant
    • C02F2101/10Inorganic compounds
    • C02F2101/16Nitrogen compounds, e.g. ammonia
    • C02F2101/166Nitrites
    • CCHEMISTRY; METALLURGY
    • C02TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
    • C02FTREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
    • C02F2101/00Nature of the contaminant
    • C02F2101/30Organic compounds
    • C02F2101/38Organic compounds containing nitrogen
    • CCHEMISTRY; METALLURGY
    • C02TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
    • C02FTREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
    • C02F2103/00Nature of the water, waste water, sewage or sludge to be treated
    • C02F2103/08Seawater, e.g. for desalination
    • CCHEMISTRY; METALLURGY
    • C02TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
    • C02FTREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
    • C02F2103/00Nature of the water, waste water, sewage or sludge to be treated
    • C02F2103/20Nature of the water, waste water, sewage or sludge to be treated from animal husbandry
    • CCHEMISTRY; METALLURGY
    • C02TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
    • C02FTREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
    • C02F2209/00Controlling or monitoring parameters in water treatment
    • C02F2209/14NH3-N
    • CCHEMISTRY; METALLURGY
    • C02TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
    • C02FTREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
    • C02F2209/00Controlling or monitoring parameters in water treatment
    • C02F2209/20Total organic carbon [TOC]
    • CCHEMISTRY; METALLURGY
    • C02TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
    • C02FTREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
    • C02F2209/00Controlling or monitoring parameters in water treatment
    • C02F2209/26H2S
    • YGENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02ATECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
    • Y02A40/00Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
    • Y02A40/80Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in fisheries management
    • Y02A40/81Aquaculture, e.g. of fish

Abstract

The invention provides a kind of sea-farming special microorganism water purification agent, including the active zeolite particle for being fixed with bacillus licheniformis;The preparation method of the active zeolite particle is:By zeolite and water with 1:10~20 mass ratio is standing and soak for 24h after stirring, and makes it disperse to be made suspension in water, is stood after crossing 300 mesh sieves, settles 24h, extracts the suspension of 10~below 15cm under liquid level, and active zeolite of the particle diameter less than 0.5 μm is obtained after centrifugal drying.Present invention also offers the preparation method and application method of the sea-farming special microorganism water purification agent.Sea-farming special microorganism water purification agent immobilization bacterium amount of the present invention is big, and bioaccumulation efficiency is high;Bacillus licheniformis activity is high, can be ingested from other biological, and can rapidly sink to the water-bed water sediment interface of cultivating pool and play a role and purify water.

Description

Sea-farming special microorganism water purification agent and preparation method thereof and application method
Technical field
The invention belongs to breeding water body field of purification, it is related to a kind of sea-farming special microorganism water purification agent and its preparation side Method and application method.
Background technology
The deterioration of breeding environment has become the key factor of restriction China mariculture industry healthy and sustainable development.Therefore, Urgent need seeks a kind of efficient, pollution-free, strong operability methods and techniques for repairing seawater pond culture environment.Traditional physics Or chemical method is not only deposited mainly using forms such as absorption, precipitation, filtering and drug disinfections using physics or chemical method The shortcomings of purification is not thorough, recycling rate of waterused is poor, input cost is big, antibiotic etc residual is also there is dynamic with aquatic The generation of antibody-resistant bacterium in object, it is final to trigger aquatic products edible safety sex chromosome mosaicism.In recent years, using microorganism come seawater of degrading Cultivating pool pollutant, the method for carrying out seawater aquaculturing pond water remediation is widely used, and achieves significant effect.
Microorganism has important effect and status in the biological group of nature, and it is both that the producer can utilize luminous energy Or simple inorganic matter production organic substance, build cell, be again consumer, analyst, be in the ecosystem material circulation and The main pusher of energy conversion, very important effect is played for the reparation of seawater aquaculturing pond environment.Additionally, many micro- Biological metabolite and cell component has special nutritional ingredient and physiological function, can promote fishes and shrimps and algae, aquatic Plant grows;Part beneficial microbe can also suppress the breeding of pathogen, reduce the generation of fish and shrimp diseases.Therefore, We can make full use of the microorganism in seawater aquaculturing pond, by beneficial microbe in the change increase water body of environmental condition Relative populations, so as to improve water quality, promote healthy growth, the development of cultivated animals, promote the sustainable development of culture fishery Exhibition, application of the modern probiotics in aquaculture grows up in this basic theory.
It is widely used the microorganism that uses in aquaculture at present and its probiotics has bacillus, photosynthetic thin Bacterium, nitrobacteria and denitrifying bacteria etc..Bacillus is gram-positive bacteria, and such bacterium can produce raw spore in resistance Son, thus there is extensive adaptability to temperature, soda acid and salt.Bacillus can not only rapidly decompose the mistake in breeding water body Surplus bait, excreta etc., while the harmful substance contents such as ammonia nitrogen, nitrite nitrogen and hydrogen sulfide can also be reduced, therefore in aquatic products Certain application has been obtained in the purification of aquaculture pond water quality.But current research concentrates on free bacillus, due to it Easily washed away by current under discharge condition, easily ingested by other biological under hydrostatic condition, and free bacillus cannot be certainly Water-deposit the interface of cultivating pool water body is so deposited to, its ability for repairing seawater aquaculturing pond substrate is limited.It is actual Upper this has become a key factor for influenceing bacillus application effect.
Be not only the repository of various chemical substances due to bottom material of pond for breeding, or plant, animal and microorganism habitat And nutrient recycling center, so bottom material of pond for breeding can consumingly influence water quality with the interaction of water, therefore want to keep Good cultivation water, must just set about, it is necessary to fully pay attention to the management of bottom material of pond for breeding from bottom material of pond for breeding improvement.Zeolite is a kind of Silicate mineral, including natural zeolite and the major class of artificial zeolite two.Wherein, natural zeolite not only has the spy of absorption pollutant Property, and in China's rich reserves, cheap, it is for use as the appropriately selected of fixation support.
The content of the invention
It is an object of the invention to be directed to deficiency of the prior art, there is provided a kind of with low cost, easy to operate and can be real The sea-farming special microorganism water purification agent of existing large-scale production, it is intended to after uniform input seawater aquaculturing pond, pond can be fallen to Seawater aquaculturing pond water-deposit interface pollutant is repaired in substrate, purification.
In order to achieve the above object, the invention provides a kind of sea-farming special microorganism water purification agent, including it is fixed with The active natural zeolite granular of bacillus licheniformis, wherein the natural zeolite is mainly from the preferable calcium boiling of common and adsorptivity Stone, sodalite, analcime etc.;
The preparation method of the active zeolite particle is:By natural zeolite and water with 1:10~20 mass ratio stirs After be standing and soak for 24h, it is disperseed to be made suspension in water, stood after crossing 300 mesh sieves, settle 24h, extract 10 under liquid level~ The suspension of below 15cm, obtains active zeolite of the particle diameter less than 0.5 μm after centrifugal drying.
This part zeolite that the present invention is used meets the requirement of the fixed bacillus licheniformis of the present invention, and can stablize outstanding During floating account is water body, or even water bottom is fallen to, meet the use requirement of the water purification agent of present invention preparation.
Present invention also offers the preparation method of above-mentioned sea-farming special microorganism water purification agent, comprise the following steps:
S1, zeolite is stirred in water after be standing and soak for 24h, it is disperseed to be made suspension in water, cross 300 mesh Stood after sieve, settle 24h, the suspension under extraction liquid level more than 10~15cm depth obtains particle diameter less than 0.5 after centrifugal drying μm active zeolite;
S2, by the Bacillus licheniformis liquid of exponential phase and active zeolite obtained in step S1 it is with volume mass ratio 1:The ratio mixing of 1~2ml/g, under aerobic conditions mixing is co-cultured, and cultivation temperature is 28~30 DEG C, and culture generation time is 24~48h;By after culture, collecting solid phase, room temperature is air-dried, and is fixed the active zeolite particle of bacillus licheniformis, i.e., It is sea-farming special microorganism water purification agent.
Under preferred embodiment, the concentration of Bacillus licheniformis liquid described in step S2 is 1 × 109~1010cfu/ml。
Product of the present invention is preserved at room temperature.
When using, used in mariculture microbial water-purifying agent of the present invention is equably sprinkling upon the water-deposition of cultivating pool water body Thing interface, according to the different breeding phase, usage amount is 50~100g/m2
Relative to prior art, advantage of the invention is that:
1st, sea-farming special microorganism water purification agent of the present invention uses active zeolite as fixation support, with lichens gemma Bacillus is immobilization object, is bred by with culture, and immobilization bacterium amount is big, and bioaccumulation efficiency is high.
2nd, bacillus licheniformis of the present invention not only can still keep activity higher after immobilization, can also be subject to active zeolite It is protected from other biological to ingest, and can rapidly sinks to pond water bottom water-deposit interface and play a role and purify water, is difficult It is lost in, extends the repair cycle.
3rd, the present invention has bigger absorption property using the zeolite after activation, can be with more efficient absorption water-deposit The pollutant of interface, the degraded beneficial to the bacillus licheniformis after fixation to pollutant improves absorption degradation pollutant Functioning efficiency.
4th, the material used by the present invention is all nontoxic, non-secondary pollution, and with low cost, is readily transported and stores, It is capable of achieving large-scale production.
Specific embodiment
With reference to specific embodiment, the present invention is described in further detail.Embodiments of the invention are using activation Zeolite fix bacillus licheniformis, and then repair cultivating pool water-deposit interface pollutant.
Embodiment 1
The preparation of active zeolite:According to zeolite:Distilled water=1:10 weight ratio stirs zeolite in distilled water After be standing and soak for 24h, make it disperse to be made suspension in water.Stood after crossing 300 mesh sieves, settle 24h, extract 15cm under liquid level Suspension more than depth, obtains active zeolite of the particle diameter less than 0.5 μm after centrifugal drying.
According to exponential phase Bacillus licheniformis liquid:Active zeolite=1:The ratio of 2 (volume mass ratios), aerobic Under the conditions of be mixed, 28~30 DEG C of culture 24h, room temperature is fixed the zeolite granular of bacillus licheniformis, room after air-drying Temperature is preserved.
At the apostichopus japonicus culture initial stage according to 100g/m2Usage amount, above-mentioned water purification agent is equably sprinkling upon apostichopus japonicus culture pond, Water purification agent can sink to the water-deposit interface of bottom of pond, reach the purpose in purifying aquaculture pond.
Experiment shows that compared with the bacillus licheniformis for adding free state, (bacillus licheniformis of free state is directly equal It is even to splash in cultivating pool;Its addition is identical with the free state bacterium amount used when preparing microbial water-purifying agent, such as:Use 100ml bacterium solutions and 100g active zeolites prepare 100g microbial water-purifying agents, in making an addition to cultivating pool, then the gemma of free state Bacillus also adds 100ml in another pond, and the two is contrasted.)
Water-deposit interface the ammonia-nitrogen content for adding the control group pond bottom of pond of the bacillus licheniformis of free state declines 34.4%, nitrite nitrogen content have dropped 3.8%, hydrogen sulfide content have dropped 20.6%, and total phosphorus content have dropped 14.8%, the content of organic carbon and organic nitrogen have dropped 21.4% and 13.2% respectively.
And water-deposit interface the ammonia nitrogen for adding activated zeolite fixation bacillus licheniformis experimental group pond bottom of pond contains Amount have dropped 54.9%, and nitrite nitrogen content have dropped 4.3%, and hydrogen sulfide content have dropped 23.7%, and total phosphorus content declines 23.7%, the content of organic carbon and organic nitrogen have dropped 31.6% and 26.4% respectively.
Embodiment 2
The preparation of active zeolite:According to zeolite:Distilled water=1:20 weight ratio stirs zeolite in distilled water After be standing and soak for 24h, make it disperse to be made suspension in water.Stood after crossing 300 mesh sieves, settle 24h, extract 10cm under liquid level Suspension more than depth, obtains active zeolite of the particle diameter less than 0.5 μm after centrifugal drying.
According to exponential phase Bacillus licheniformis liquid:Active zeolite=1:The ratio of 1 (volume mass ratio), aerobic Under the conditions of be mixed, 28~30 DEG C of culture 36h, room temperature is fixed the zeolite granular of bacillus licheniformis, room after air-drying Temperature is preserved.
In the apostichopus japonicus culture later stage according to 75g/m2Usage amount, above-mentioned water purification agent is equably sprinkling upon apostichopus japonicus culture pond, Water purification agent can sink to the water-deposit interface of bottom of pond, reach the purpose in purifying aquaculture pond.
Experiment shows, the bacillus licheniformis with addition free state is compared, add free state bacillus licheniformis it is right Water-deposit interface ammonia-nitrogen content according to group pond bottom of pond have dropped 31.6%, and nitrite nitrogen content have dropped 4.1%, Hydrogen sulfide content have dropped 23.2%, and total phosphorus content have dropped 13.7%, and the content of organic carbon and organic nitrogen have dropped respectively 19.6% and 25.2%.
Add water-deposit interface ammonia-nitrogen content that activated zeolite fixes bacillus licheniformis experimental group pond bottom of pond 43.8% is have dropped, nitrite nitrogen content declines 5.9%, and hydrogen sulfide content have dropped 27.7%, and total phosphorus content have dropped 21.6%, the content of organic carbon and organic nitrogen have dropped 28.2% and 33.8% respectively.
Embodiment 3
The preparation of active zeolite:According to zeolite: distilled water=1:15 weight ratio stirs zeolite in distilled water After be standing and soak for 30h, make it disperse to be made suspension in water.Cross after 200 mesh sieves and stand, settle 24h, extract 15cm depth with On suspension, particle diameter is obtained after centrifugal drying less than 0.5 μm of active zeolite.
According to exponential phase Bacillus licheniformis liquid: active zeolite=1: the ratio of 2 (volume mass ratios), aerobic Under the conditions of be mixed, 28~30 DEG C of culture 36h, room temperature is fixed the zeolite granular of bacillus licheniformis, room after air-drying Temperature is preserved.
According to 50g/m during culture of Penaeus vannamei2Usage amount, above-mentioned water purification agent is equably sprinkling upon prawn culturing Pond, water purification agent can sink to the water-deposit interface of bottom of pond, reach the purpose in purifying aquaculture pond.
Experiment shows, the bacillus licheniformis with addition free state is compared, add free state bacillus licheniformis it is right Water-deposit interface ammonia-nitrogen content according to group pond bottom of pond have dropped 18.7%, and nitrite nitrogen content have dropped 2.5%, Hydrogen sulfide content have dropped 16.9%, and total phosphorus content have dropped 18.8%, and the content of organic carbon and organic nitrogen have dropped respectively 20.3% and 18.4%.
Add water-deposit interface ammonia-nitrogen content that activated zeolite fixes bacillus licheniformis experimental group pond bottom of pond 25.9% is have dropped, nitrite nitrogen content have dropped 2.7%, and hydrogen sulfide content have dropped 24.1%, and total phosphorus content have dropped 25.8%, the content of organic carbon and organic nitrogen have dropped 26.3% and 27.8% respectively.
Embodiment 4
The preparation of active zeolite:According to zeolite: distilled water=1: 20 weight ratio stirs zeolite in distilled water After be standing and soak for 36h, make it disperse to be made suspension in water.Cross after 300 mesh sieves and stand, settle 24h, extract 15cm depth with On suspension, particle diameter is obtained after centrifugal drying less than 0.5 μm of active zeolite.
According to exponential phase Bacillus licheniformis liquid: active zeolite=1: the ratio of 1 (volume mass ratio), aerobic Under the conditions of be mixed, 28~30 DEG C of culture 48h, room temperature is fixed the zeolite granular of bacillus licheniformis, room after air-drying Temperature is preserved.
According to 50g/m during turbot is cultivated2Usage amount, by above-mentioned water purification agent be equably sprinkling upon hybridized prussian carp cultivation Pond, water purification agent can sink to the water-deposit interface of bottom of pond, reach the purpose in purifying aquaculture pond.
Experiment shows, the bacillus licheniformis with addition free state is compared, add free state bacillus licheniformis it is right Water-deposit interface ammonia-nitrogen content according to group pond bottom of pond have dropped 20.5%, and nitrite nitrogen content have dropped 5.3%, Hydrogen sulfide content have dropped 23.8%, and total phosphorus content have dropped 21.1%, and the content of organic carbon and organic nitrogen have dropped respectively 20.9% and 17.6%.
Add water-deposit interface ammonia-nitrogen content that activated zeolite fixes bacillus licheniformis experimental group pond bottom of pond 28.5% is have dropped, nitrite nitrogen content have dropped 9.2%, and hydrogen sulfide content have dropped 33.2%, and total phosphorus content have dropped 32.7%, the content of organic carbon and organic nitrogen have dropped 30.9% and 35.5% respectively.
The above, the only present invention preferably specific embodiment, but protection scope of the present invention is not limited thereto, Any one skilled in the art in the technical scope of present disclosure, technology according to the present invention scheme and its Inventive concept is subject to equivalent or change, should all be included within the scope of the present invention.

Claims (4)

1. a kind of sea-farming special microorganism water purification agent, it is characterised in that the activation including being fixed with bacillus licheniformis is boiled Stone particle;
The preparation method of the active zeolite particle is:By zeolite and water with 1:10~20 mass ratio stands leaching after stirring Bubble 24h, makes it disperse to be made suspension in water, is stood after crossing 300 mesh sieves, settles 24h, extracts 10~below 15cm under liquid level Suspension, particle diameter is obtained after centrifugal drying less than 0.5 μm of active zeolite.
2. the preparation method of sea-farming special microorganism water purification agent described in claim 1, it is characterised in that including following step Suddenly:
S1, zeolite is stirred in water after be standing and soak for 24h, it is disperseed to be made suspension in water, after crossing 300 mesh sieves Stand, settle 24h, the suspension under extraction liquid level more than 10~15cm depth obtains particle diameter and is less than 0.5 μm after centrifugal drying Active zeolite;
S2, by the Bacillus licheniformis liquid of exponential phase and active zeolite obtained in step S1 with volume mass ratio be 1:1 The ratio mixing of~2ml/g, under aerobic conditions mixing is co-cultured, and cultivation temperature is 28~30 DEG C, and culture generation time is 24 ~48h;By after culture, collecting solid phase, room temperature is air-dried, and is fixed the active zeolite particle of bacillus licheniformis, as Sea-farming special microorganism water purification agent.
3. the preparation method of sea-farming special microorganism water purification agent according to claim 2, it is characterised in that step S2 institutes The concentration for stating Bacillus licheniformis liquid is 1 × 109~1010cfu/ml。
4. the application method of sea-farming special microorganism water purification agent described in claim 1, it is characterised in that the sea-farming Special microorganism water purification agent is equably sprinkling upon the water-deposit interface of cultivating pool water body, and usage amount is 50~100g/m2
CN201710095159.XA 2017-02-22 2017-02-22 Sea-farming special microorganism water purification agent and preparation method thereof and application method Pending CN106745821A (en)

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CN108083595A (en) * 2017-12-21 2018-05-29 中国水产科学研究院珠江水产研究所 A kind of restorative procedure of fresh water richness nitrogen cultivating pool bed mud

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