CN105062915A - Microecological preparation for degrading N and P (nitrogen and phosphorus) - Google Patents
Microecological preparation for degrading N and P (nitrogen and phosphorus) Download PDFInfo
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- CN105062915A CN105062915A CN201510434800.9A CN201510434800A CN105062915A CN 105062915 A CN105062915 A CN 105062915A CN 201510434800 A CN201510434800 A CN 201510434800A CN 105062915 A CN105062915 A CN 105062915A
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Abstract
The invention relates to a microecological preparation for degrading N and P (nitrogen and phosphorus). The microecological preparation is prepared by mainly mixing bacillus natto, photosynthetic bacteria, nitrifying bacteria and lactic acid bacteria at the weight ratio of 1: 2: 10: 2. The product disclosed by the invention is high in live bacterium concentration, stable in quality, easy in preservation and convenient in transport. The product, as a microecological preparation, is safe and environment-friendly, and the product is widely applicable to water quality improvement of seawater and fresh water culture ponds and nursery ponds and capable of promoting ecological balance of environment.
Description
Technical field
The present invention relates to a kind of probiotics, particularly the probiotics of a kind of can degrade N, P.Be a kind of energy regulating water quality, decompose the Water body cleansing agent of Organic substance in water and objectionable impurities.
Background technology
China is a hydrocoles cultivation big country, and in recent years, along with the raising of living standards of the people, hydrocoles aquaculture development is rapid.Due to the increase of cultivation density and the deterioration of water ecological setting, various hydrocoles disease and pest is on the rise.In order to prevention and elimination of disease and pests, improve culture benefit, producer blindly pursues high-density breeding, and uses microbiotic, sterilizing agent, sterilant in a large number, even uses forbidding medicine and severe toxicity, persistent pesticide.Bring such as water environment pollution, the increase of pathogenic agent resistance, degradation series of negative effect under quality product thereupon.
The deterioration of water quality is the major obstacle affecting hydrocoles cultivation survival rate and output, and other objectionable impurities in the Metabolism Excretion thing that hydrocoles produces in process of growth, organic resolvent and water, to the toxic effect of fish.Dissolved oxygen amount in water body also has direct relation with the health of fish, when the content of oxygen is few, the content of harmful bacteria is just many.Numerous scholars has recognized that aquatic products disease is very close with water body environment relation, if do not improved water quality, aquatic products health just can not be guaranteed, water ecology will be destroyed, disease can get more and more, output can be restricted greatly, and the health of the mankind also can be on the hazard because of edible unsafe fishery products simultaneously.Therefore the purification of culture environment of aquatic products has become gordian technique and the study hotspot of aquaculture production sustainable development.
As the saying goes: " breed fish and be foster water ".So, will under the current conditions of high-density breeding, reduce and avoid the incidence of disease, just water quality must be improved, realize green cultivation, the practice that for many years chemicals such as the using sulfated zinc of fisherman is harnessed the river illustrates, the road that chemical process supports water is impracticable, only can cause more and more serious heavy metal contamination and the generation of more serious disease.Thus, arise at the historic moment in probiotics market.
Probiotics is under the guidance of microecology theory, adjustment ecologic disturbance, keep microecological balance, improve physiological viable bacteria goods (microorganism) and the meta-bolites thereof of host health level or the state that improves health in environment and promote the material goods that these physiology flora growth are bred.For water environment treatment, probiotics have nontoxic, have no side effect, noresidue and the feature such as not develop immunity to drugs, can effectively improve breeding ecological environment, regulate Water quality, eliminate the immunizing power that ammonia, nitrogen etc. pollute, enhancing cultivates object, in healthy aquaculture, play very important effect.Its action principle utilizes between similar biology to there is Competition, a certain amount ofly being conducive to water quality improvement when optionally dropping in aquaculture water, Organic substance in water and objectionable impurities can be decomposed, and to aquaculture organism without after the microbial preparation endangered and microbial strains, set up a kind of new equilibrium relationship, the growth of pathogenic bacteria is inhibited, water quality improves, and aquaculture organism is grown up healthy and sound.
Summary of the invention
The object of the present invention is to provide the probiotics of a kind of degrade N, P.N and P concentration in water body is reduced mainly through methods such as physics, chemistry and microorganisms.Along with people, in water body, the microorganism of existence and the deep of the research of cultivated animals probiotic bacterium grow up microbial process naturally, be explore from ecological angle a kind ofly base oneself upon self-regeneration, long-acting, safe method, have natural, nontoxic, have no side effect, pollution-free, noresidue, the feature such as safe and reliable.
Object of the present invention: by genus bacillus, photosynthetic bacterium, nitrobacteria and lactobacillus culture condition and the research to water purification thereof, develop a kind of tetragenous viable bacteria preparation that can be used for purifying pond culturing water, and the production technique of this product and result of use are verified, to solve produce in the problem that raises at cultivation middle and later periods N, P of Aquacultural water.
Technical scheme of the present invention: the probiotics of a kind of degrade N, P, it is characterized in that: primarily of bacillus natto, photosynthetic bacterium, nitrobacteria, milk-acid bacteria by weight proportion 1:2:10:2 mix, bacteria suspension is heated 5min by bacillus natto in 85 DEG C of water-baths, with the inoculum size of volume ratio 5% access 100L seeding tank, loading amount coefficient is 60% (v/v), mixing speed 150r/min, air flow 1-2m ∽ 3/h, cultivates 17h for 37 DEG C.Seed culture fluid is with the inoculum size of volume ratio 10% access 1m ~ 3 fermentor tank, and loading amount coefficient is 65% (v/v), mixing speed 150r/min, air flow 1.5m ~ 3/h, cultivates 20h for 37 DEG C; According to the situation of foam in culturing process, add soya-bean oil or steep enemy and carry out froth breaking, prevent because foam escapes to select fermentor tank and cause living contaminants; Photosynthetic bacterium test tube or small-sized saline bottle nutrient solution are with 10% inoculum size access 5L triangular flask, and loading amount coefficient is 80% (v/v), 2000Lx illumination, 28-30 DEG C of quiescent culture 5 days; Seed culture fluid is accessed 20 50 kilograms of plastic tanks with the inoculum size of volume ratio 10%, and loading amount coefficient is 80% (v/v), indoor controlled light 2000Lx, temperature 28-30 DEG C, quiescent culture 5-10 days is garnet to nutrient solution, has a large amount of bacterial sediment at the bottom of bucket; Nitrobacteria triangular flask bacterium liquid is with the inoculum size of volume ratio 5% access 100L seeding tank, and loading amount coefficient is 60% (v/v), mixing speed 150r/min, air flow 0.5m ~ 3/h, cultivates 5d for 28 DEG C; Seed culture fluid is connect 1m ~ 3 fermentor tank with the inoculum size of volume ratio 5%, and loading amount coefficient is 60% (v/v), mixing speed 150r/min, air flow 0.5m ~ 3/h; Cultivate 5d for 28 DEG C; Milk-acid bacteria triangular flask bacterium liquid is with the inoculum size of volume ratio 5% access 100L seeding tank, and loading amount coefficient is 70% (v/v), mixing speed 100r/min, air flow 0.5m ~ 3/h, cultivates 48h for 40 DEG C; Seed culture fluid is connect 1m ~ 3 fermentor tank with the inoculum size of volume ratio 5%, and loading amount coefficient is 70% (v/v), mixing speed 100r/min, air flow 1m ~ 3/h, cultivates 48h for 40 DEG C.
Bacillus natto of the present invention, photosynthetic bacterium, nitrobacteria, milk-acid bacteria obtain after the multiple beneficial strain culturing and fermentation of the meticulous seed selection of nature.
Product viable bacteria concentration of the present invention is high, steady quality, easily preservation, convenient transportation.Can be used for water transfer, rich water, strengthening bait, eliminate and, weed survival rate etc. stress be improved, be specially adapted to feed coefficient, aging pond, nursery and wintering pond and use.The invention belongs to probiotics, safety, environmental protection, be widely used in the water quality improvement of sea, freshwater aquiculture pond and nursery pond, promote the eubiosis of culture environment of aquatic products.
(1) can ammonia nitrogen, nitrite, organism and some toxic substances in fast degradation water, improve water quality, enhance the transparency, and there is effect that is efficient, rapid detoxification, promote the cultivation water environment eubiosis.
(2) suppress the excessive breeding of harmful algae in culturing pool, the nutrient source of vibrios capable of blocking, luminescent bacteria suppresses its growth, keeps water conditioning.
(3) suppress pathogenic bacteria, form useful dominant microflora, stimulating immune system, improve immunizing power.
(4) provide nutrition, promoting digestion, improve organism metabolism, obviously promote hydrocoles growth.
Industrialization prospect: current, has risen the upsurge that one goes back to nature in world wide, people's pay attention to day by day ecological safety and food safety, defines edible aquatic products requirement " high-quality, low medicine residual, green non-pollution " this consumption tide.Aquaculture family has defined the medication orientation reducing fishery drugs residue, reduce water pollution, and this provides wide development space just to the degraded of aquaculture probiotics N, P.
Innovative point of the present invention:
(1) product of the present invention shows N and P Degrading experiment result in aquaculture water, and the synergy of milk-acid bacteria, photosynthetic bacterium, nitrobacteria, bacillus natto to the degradation effect of N, P significantly, fast.When preparation addition be respectively 1.5,3.04 .5kg/hm2 time, the clearance of 5 days N is respectively the clearance of 52%, 80%, 74%, P close to 100%, and result is all significantly higher than the clearance of N when adding bacterial strain single with dosage, P.
(2) bacillus natto in product of the present invention, photosynthetic bacterium, nitrobacteria, milk-acid bacteria obtain after the multiple beneficial strain culturing and fermentation of the meticulous seed selection of nature.Compositely after space seed selection form, performance is the 4-6 of common bacterial classification.
(3) the present invention adopts the technology such as liquid submerged fermentation, spraying, and free-running property is good, and product performance are more stable, and the quality guaranteed period is longer.
(4) the present invention is from ecological angle, utilizes microbiological deterioration aquaculture water N, P content, its have natural, nontoxic, have no side effect, pollution-free, noresidue, safe and reliable superiority; Utilize beneficial microorganism to improve water quality, suppress pathogenic bacteria, reduce disease.
Embodiment
Embodiment 1
The probiotics of a kind of degrade N, P is on the impact of cultivating pool environment.
1, pond is tested
Test is carried out in 1,2,6 ponds of Linxiang Huang Gai Garden Pond plant.Pond area is 1.5 meters, and the depth of water 1.5 meters, mainly cultivates the fingerling such as grass carp, crucian, and 1,2 is the test pool, fishpond, and No. 6 is contrast pond.Test period is July 03 to 07, and weather is fine, water temperature 24-27 degree.
2, in use sample 96 hours, the ammonia nitrogen velocity of variation that the velocity of variation of 1 and 2 test pond ammonia nitrogens are respectively-3.31%-4.13% and-5.44%-4.76. and contrast pond 6 is-3.60%-5.04%.Result shows, after probiotics of the present invention uses, the ammonia nitrogen of cultivating pool has obvious change, the results are shown in Table
After probiotics of the present invention uses, the change of cultivating pool nitrite
3, after probiotics of the present invention uses, in 96 hours, the nitrite velocity of variation that the velocity of variation of 1 and 2 test pond nitrite are respectively-3.76%-4.51% and-5.88%-4.90. and contrast pond 6 is-6.98%-7.75%.Result shows, after probiotics of the present invention uses, the nitrite of cultivating pool has obvious change, the results are shown in Table
After probiotics of the present invention uses, the change of cultivating pool algae
4, after probiotics of the present invention uses, in 96 hours, the velocity of variation of 1 and 2 test pond algae is respectively-3.76%-4.51% and-5.88%-4.90. and contrasts the algae change rate in pond 6 for-6.98%-7.75%.Result shows, after probiotics of the present invention uses, the algae of cultivating pool has obvious change, the results are shown in Table
Result shows, after probiotics of the present invention uses, in 24 hours, the ammonia nitrogen of cultivating pool, nitrite and algae have obvious change.
Embodiment 2
The probiotics of a kind of degrade N, P is to the productivity expanding test of control hydrocoles disease.
Paid a home visit by fish disease door, select to make a definite diagnosis the plant suffering from bacteriosis, by pond water volume computing dosage, being dissolved in by medicine in suitable quantity of water, evenly splashes in full pond.Every day 1 time or 2-3 days be continuously 1 course for the treatment of.Meanwhile, observe and check incidence, record is added up and is treated first 3 days, treatment neutralization treatment dead kind, the quantity of latter continuous 7-10 days.
1, grass carp adult fish culture fish pond, Hong Tang village, Huzhou City of Zhejiang Province Shuan Lin town, pond area is respectively 17 mu and 15 mu, average depth 2 meters, main cultivation grass carp, and take foster crucian, silver carp, variegated carp etc., pond death every day on 3-June 5 two in June about 15 kilograms of grass carps, the cardinal symptom side of dead grass carp, the hemorrhage inflammation of belly, scale comes off; Fin ray end rots, scatters, and fin base portion is rubescent, and enteron aisle is without food, and the congested inflammation of intestines wall, be full of mucus in enteric cavity, there is yellow ascites in abdominal cavity.Be diagnosed as grass carp erythroderma and enteritis disease complication after diagnosing, June 6 is with the precious full pond float of water purification, dead grass carp reduces gradually, about dead 10 kilograms of June 7, about dead 8 kilograms of June 8, about dead 3 kilograms of June 9, only there was a small amount of death on June 10, again used the precious full pond float of water purification on June 11, death on June 12 stops completely, and Feeding in Grass Carp recovers normal.Result shows, probiotics of the present invention has good preventive and therapeutic effect to bacteriosises such as the erythroderma of grass carp and rotted gill diseases.
2, culture of Penaeus vannamei field, Zhejiang Hangzhou Xianoshan City Tidal Flat Inning Area, morbidity pond culture area is 18 mu, the depth of water 1 meter, pond salinity water is about and finds this pond Penaeus vannamei morbidity at the beginning of the 3-5.8 month, the cardinal symptom of morbidity is that shrimp is swum in shoaling water place, and appendage such as swimmeret reddens, gill filament swelling, empty stomach, through bacteria distribution culture & identification, making a definite diagnosis pathogenic bacteria is vibrios.Death about 3 kilograms before medication, food obviously reduces.August 9, mix bait simultaneously and to throw something and feed Enrofloxacin, throw something and feed continuously three days, during administration, daily ration, feeding quantity reduced by half with the precious full pond float of water purification.August, 10-August 12, sick shrimp number obviously reduced, Mean Death about 1 kilogram, dead basic stopping on August 13, and August 15, food recovered normal substantially.Result shows that the vibriosis of probiotics of the present invention to Penaeus vannamei has good preventive and therapeutic effect.
3, Yuhang, Hangzhou production turtle-breeding field, cultured area 20 mu, soft-shelled turtle mean body weight 0.5kg, started ulcus perforans disease occurs mid-August, and had a small amount of death, every day about dead 10-20 between period of disease.Within the 3rd day after dead generation, use the precious full pond float of water purification, re-use every other day once.After medication, dead soft-shelled turtle number obviously reduces, and about dead 3-5 of every day, within after medication the 7th day, stops death completely.Result shows that water purification treasured has good preventive and therapeutic effect to cavern disease in soft-shelled turtle.
Result shows: in aquaculture of aquatic animal is produced, the bacteriosis of probiotics of the present invention to aquatic animals such as fish, Shrimp waste and Trionyx sinensis (Wiegmann) has good preventive and therapeutic effect.
Claims (2)
1. a degraded N, the probiotics of P, it is characterized in that: primarily of bacillus natto, photosynthetic bacterium, nitrobacteria, milk-acid bacteria by weight proportion 1:2:10:2 mixes, bacteria suspension is heated 5min by bacillus natto in 85 DEG C of water-baths, with the inoculum size of volume ratio 5% access 100L seeding tank, loading amount coefficient is 60% (v/v), mixing speed 150r/min, air flow 1-2m ~ 3/h, cultivate 17h for 37 DEG C, seed culture fluid is with the inoculum size of volume ratio 10% access 1m ~ 3 fermentor tank, loading amount coefficient is 65% (v/v), mixing speed 150r/min, air flow 1.5m ~ 3/h, cultivate 20h for 37 DEG C, according to the situation of foam in culturing process, add soya-bean oil or steep enemy and carry out froth breaking, prevent because foam escapes to select fermentor tank and cause living contaminants, photosynthetic bacterium test tube or small-sized saline bottle nutrient solution are with 10% inoculum size access 5L triangular flask, and loading amount coefficient is 80% (v/v), 2000Lx illumination, 28-30 DEG C of quiescent culture 5 days, seed culture fluid is accessed 20 50 kilograms of plastic tanks with the inoculum size of volume ratio 10%, and loading amount coefficient is 80% (v/v), indoor controlled light 2000Lx, temperature 28-30 DEG C, quiescent culture 5-10 days is garnet to nutrient solution, has a large amount of bacterial sediment at the bottom of bucket, nitrobacteria triangular flask bacterium liquid is with the inoculum size of volume ratio 5% access 100L seeding tank, and loading amount coefficient is 60% (v/v), mixing speed 150r/min, air flow 0.5m ~ 3/h, cultivates 5d for 28 DEG C, seed culture fluid is connect 1m ~ 3 fermentor tank with the inoculum size of volume ratio 5%, and loading amount coefficient is 60% (v/v), mixing speed 150r/min, air flow 0.5m ~ 3/h, cultivate 5d for 28 DEG C, milk-acid bacteria triangular flask bacterium liquid is with the inoculum size of volume ratio 5% access 100L seeding tank, and loading amount coefficient is 70% (v/v), mixing speed 100r/min, air flow 0.5m ~ 3/h, cultivates 48h for 40 DEG C, seed culture fluid is connect 1m ∽ 3 fermentor tank with the inoculum size of volume ratio 5%, and loading amount coefficient is 70% (v/v), mixing speed 100r/min, air flow 1m ~ 3/h, cultivates 48h for 40 DEG C.
2. the probiotics of a kind of degrade N, P according to claim 1, is characterized in that: described bacillus natto, photosynthetic bacterium, nitrobacteria, milk-acid bacteria obtain after the multiple beneficial strain culturing and fermentation of the meticulous seed selection of nature.
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN109336263A (en) * | 2018-10-30 | 2019-02-15 | 江苏世邦生物工程科技有限公司 | Microorganism water conditioner and preparation method thereof |
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| CN109336263A (en) * | 2018-10-30 | 2019-02-15 | 江苏世邦生物工程科技有限公司 | Microorganism water conditioner and preparation method thereof |
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