CN107673481A - A kind of water body purification of aquaculture agent and preparation method thereof - Google Patents
A kind of water body purification of aquaculture agent and preparation method thereof Download PDFInfo
- Publication number
- CN107673481A CN107673481A CN201710908790.7A CN201710908790A CN107673481A CN 107673481 A CN107673481 A CN 107673481A CN 201710908790 A CN201710908790 A CN 201710908790A CN 107673481 A CN107673481 A CN 107673481A
- Authority
- CN
- China
- Prior art keywords
- agent
- parts
- water body
- bacillus
- aquaculture
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
- 239000003795 chemical substances by application Substances 0.000 title claims abstract description 109
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 title claims abstract description 80
- 238000009360 aquaculture Methods 0.000 title claims abstract description 36
- 238000000746 purification Methods 0.000 title claims abstract description 34
- 244000144974 aquaculture Species 0.000 title claims abstract description 33
- 238000002360 preparation method Methods 0.000 title claims abstract description 29
- 241000894006 Bacteria Species 0.000 claims abstract description 97
- 230000000243 photosynthetic effect Effects 0.000 claims abstract description 61
- 241000193830 Bacillus <bacterium> Species 0.000 claims abstract description 29
- 239000001963 growth medium Substances 0.000 claims abstract description 27
- 239000000919 ceramic Substances 0.000 claims abstract description 23
- FRXSZNDVFUDTIR-UHFFFAOYSA-N 6-methoxy-1,2,3,4-tetrahydroquinoline Chemical compound N1CCCC2=CC(OC)=CC=C21 FRXSZNDVFUDTIR-UHFFFAOYSA-N 0.000 claims description 43
- 238000000855 fermentation Methods 0.000 claims description 42
- 230000004151 fermentation Effects 0.000 claims description 42
- 239000000463 material Substances 0.000 claims description 29
- FAPWRFPIFSIZLT-UHFFFAOYSA-M Sodium chloride Chemical compound [Na+].[Cl-] FAPWRFPIFSIZLT-UHFFFAOYSA-M 0.000 claims description 28
- 239000002609 medium Substances 0.000 claims description 27
- 238000011218 seed culture Methods 0.000 claims description 26
- NLXLAEXVIDQMFP-UHFFFAOYSA-N Ammonia chloride Chemical compound [NH4+].[Cl-] NLXLAEXVIDQMFP-UHFFFAOYSA-N 0.000 claims description 24
- TWRXJAOTZQYOKJ-UHFFFAOYSA-L Magnesium chloride Chemical compound [Mg+2].[Cl-].[Cl-] TWRXJAOTZQYOKJ-UHFFFAOYSA-L 0.000 claims description 24
- ZPWVASYFFYYZEW-UHFFFAOYSA-L dipotassium hydrogen phosphate Chemical compound [K+].[K+].OP([O-])([O-])=O ZPWVASYFFYYZEW-UHFFFAOYSA-L 0.000 claims description 24
- 239000000284 extract Substances 0.000 claims description 22
- 229920001661 Chitosan Polymers 0.000 claims description 18
- 229920002678 cellulose Polymers 0.000 claims description 17
- 238000005286 illumination Methods 0.000 claims description 17
- 239000001913 cellulose Substances 0.000 claims description 16
- 235000011347 Moringa oleifera Nutrition 0.000 claims description 14
- 239000011780 sodium chloride Substances 0.000 claims description 14
- UXVMQQNJUSDDNG-UHFFFAOYSA-L Calcium chloride Chemical compound [Cl-].[Cl-].[Ca+2] UXVMQQNJUSDDNG-UHFFFAOYSA-L 0.000 claims description 12
- 239000001888 Peptone Substances 0.000 claims description 12
- 108010080698 Peptones Proteins 0.000 claims description 12
- VMHLLURERBWHNL-UHFFFAOYSA-M Sodium acetate Chemical compound [Na+].CC([O-])=O VMHLLURERBWHNL-UHFFFAOYSA-M 0.000 claims description 12
- 235000019270 ammonium chloride Nutrition 0.000 claims description 12
- 235000015278 beef Nutrition 0.000 claims description 12
- 239000001110 calcium chloride Substances 0.000 claims description 12
- 229910001628 calcium chloride Inorganic materials 0.000 claims description 12
- 229940041514 candida albicans extract Drugs 0.000 claims description 12
- 229910001629 magnesium chloride Inorganic materials 0.000 claims description 12
- 229910000402 monopotassium phosphate Inorganic materials 0.000 claims description 12
- 235000019796 monopotassium phosphate Nutrition 0.000 claims description 12
- 235000019319 peptone Nutrition 0.000 claims description 12
- PJNZPQUBCPKICU-UHFFFAOYSA-N phosphoric acid;potassium Chemical compound [K].OP(O)(O)=O PJNZPQUBCPKICU-UHFFFAOYSA-N 0.000 claims description 12
- 239000001632 sodium acetate Substances 0.000 claims description 12
- 235000017281 sodium acetate Nutrition 0.000 claims description 12
- 239000012138 yeast extract Substances 0.000 claims description 12
- 241000190984 Rhodospirillum rubrum Species 0.000 claims description 11
- 239000004005 microsphere Substances 0.000 claims description 11
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 claims description 10
- 241000316848 Rhodococcus <scale insect> Species 0.000 claims description 10
- 239000000843 powder Substances 0.000 claims description 8
- OKTJSMMVPCPJKN-UHFFFAOYSA-N Carbon Chemical compound [C] OKTJSMMVPCPJKN-UHFFFAOYSA-N 0.000 claims description 6
- 239000000758 substrate Substances 0.000 claims description 6
- 239000002131 composite material Substances 0.000 claims description 5
- 241000193749 Bacillus coagulans Species 0.000 claims description 4
- 241000194108 Bacillus licheniformis Species 0.000 claims description 4
- 241000726221 Gemma Species 0.000 claims description 4
- 241000589614 Pseudomonas stutzeri Species 0.000 claims description 4
- 229940054340 bacillus coagulans Drugs 0.000 claims description 4
- 239000010439 graphite Substances 0.000 claims description 4
- 229910002804 graphite Inorganic materials 0.000 claims description 4
- 238000000034 method Methods 0.000 claims description 4
- 238000001035 drying Methods 0.000 claims description 3
- 239000002994 raw material Substances 0.000 claims description 3
- 241000233866 Fungi Species 0.000 claims description 2
- -1 are uniformly mixed Substances 0.000 claims description 2
- 244000179886 Moringa oleifera Species 0.000 claims 2
- 244000025254 Cannabis sativa Species 0.000 claims 1
- 241000589516 Pseudomonas Species 0.000 claims 1
- 238000001514 detection method Methods 0.000 claims 1
- 244000005700 microbiome Species 0.000 abstract description 7
- 238000009395 breeding Methods 0.000 abstract description 6
- 230000001488 breeding effect Effects 0.000 abstract description 6
- 201000010099 disease Diseases 0.000 abstract description 4
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 abstract description 4
- 230000000694 effects Effects 0.000 abstract description 2
- 230000002265 prevention Effects 0.000 abstract 1
- 235000010980 cellulose Nutrition 0.000 description 15
- IJGRMHOSHXDMSA-UHFFFAOYSA-N Atomic nitrogen Chemical compound N#N IJGRMHOSHXDMSA-UHFFFAOYSA-N 0.000 description 12
- 241000220215 Moringa Species 0.000 description 12
- 241000190950 Rhodopseudomonas palustris Species 0.000 description 10
- 244000063299 Bacillus subtilis Species 0.000 description 8
- 235000014469 Bacillus subtilis Nutrition 0.000 description 8
- 238000005516 engineering process Methods 0.000 description 8
- 239000007788 liquid Substances 0.000 description 8
- IOVCWXUNBOPUCH-UHFFFAOYSA-M Nitrite anion Chemical compound [O-]N=O IOVCWXUNBOPUCH-UHFFFAOYSA-M 0.000 description 7
- 229910052757 nitrogen Inorganic materials 0.000 description 6
- 238000004321 preservation Methods 0.000 description 6
- QCVGEOXPDFCNHA-UHFFFAOYSA-N 5,5-dimethyl-2,4-dioxo-1,3-oxazolidine-3-carboxamide Chemical compound CC1(C)OC(=O)N(C(N)=O)C1=O QCVGEOXPDFCNHA-UHFFFAOYSA-N 0.000 description 5
- 241000251468 Actinopterygii Species 0.000 description 5
- 102000002322 Egg Proteins Human genes 0.000 description 5
- 108010000912 Egg Proteins Proteins 0.000 description 5
- 150000001413 amino acids Chemical class 0.000 description 5
- 235000014103 egg white Nutrition 0.000 description 5
- 210000000969 egg white Anatomy 0.000 description 5
- 230000002906 microbiologic effect Effects 0.000 description 5
- QGZKDVFQNNGYKY-UHFFFAOYSA-N Ammonia Chemical compound N QGZKDVFQNNGYKY-UHFFFAOYSA-N 0.000 description 4
- XKMRRTOUMJRJIA-UHFFFAOYSA-N ammonia nh3 Chemical compound N.N XKMRRTOUMJRJIA-UHFFFAOYSA-N 0.000 description 4
- 238000006243 chemical reaction Methods 0.000 description 4
- 239000003814 drug Substances 0.000 description 4
- 238000009629 microbiological culture Methods 0.000 description 4
- 239000000203 mixture Substances 0.000 description 4
- 239000008188 pellet Substances 0.000 description 4
- 239000011782 vitamin Substances 0.000 description 4
- 229930003231 vitamin Natural products 0.000 description 4
- 229940088594 vitamin Drugs 0.000 description 4
- 235000013343 vitamin Nutrition 0.000 description 4
- 150000003722 vitamin derivatives Chemical class 0.000 description 4
- ZXLOSLWIGFGPIU-UHFFFAOYSA-N 1-ethyl-3-methyl-1,2-dihydroimidazol-1-ium;acetate Chemical compound CC(O)=O.CCN1CN(C)C=C1 ZXLOSLWIGFGPIU-UHFFFAOYSA-N 0.000 description 3
- 241000143060 Americamysis bahia Species 0.000 description 3
- 241000194103 Bacillus pumilus Species 0.000 description 3
- QVGXLLKOCUKJST-UHFFFAOYSA-N atomic oxygen Chemical compound [O] QVGXLLKOCUKJST-UHFFFAOYSA-N 0.000 description 3
- 230000001112 coagulating effect Effects 0.000 description 3
- 235000019441 ethanol Nutrition 0.000 description 3
- 239000000706 filtrate Substances 0.000 description 3
- 239000002054 inoculum Substances 0.000 description 3
- 230000002503 metabolic effect Effects 0.000 description 3
- 239000001301 oxygen Substances 0.000 description 3
- 229910052760 oxygen Inorganic materials 0.000 description 3
- 239000000047 product Substances 0.000 description 3
- 230000004083 survival effect Effects 0.000 description 3
- 238000012360 testing method Methods 0.000 description 3
- YBJHBAHKTGYVGT-ZKWXMUAHSA-N (+)-Biotin Chemical compound N1C(=O)N[C@@H]2[C@H](CCCCC(=O)O)SC[C@@H]21 YBJHBAHKTGYVGT-ZKWXMUAHSA-N 0.000 description 2
- 241000252230 Ctenopharyngodon idella Species 0.000 description 2
- 229910021529 ammonia Inorganic materials 0.000 description 2
- 244000052616 bacterial pathogen Species 0.000 description 2
- 230000037396 body weight Effects 0.000 description 2
- 229910052799 carbon Inorganic materials 0.000 description 2
- 238000011109 contamination Methods 0.000 description 2
- 238000004132 cross linking Methods 0.000 description 2
- 239000008367 deionised water Substances 0.000 description 2
- 229910021641 deionized water Inorganic materials 0.000 description 2
- 238000011161 development Methods 0.000 description 2
- 210000003608 fece Anatomy 0.000 description 2
- OVBPIULPVIDEAO-LBPRGKRZSA-N folic acid Chemical compound C=1N=C2NC(N)=NC(=O)C2=NC=1CNC1=CC=C(C(=O)N[C@@H](CCC(O)=O)C(O)=O)C=C1 OVBPIULPVIDEAO-LBPRGKRZSA-N 0.000 description 2
- 235000013305 food Nutrition 0.000 description 2
- 239000003365 glass fiber Substances 0.000 description 2
- 230000012010 growth Effects 0.000 description 2
- 238000010438 heat treatment Methods 0.000 description 2
- 239000002608 ionic liquid Substances 0.000 description 2
- 238000005374 membrane filtration Methods 0.000 description 2
- 244000000010 microbial pathogen Species 0.000 description 2
- 239000005416 organic matter Substances 0.000 description 2
- 239000002304 perfume Substances 0.000 description 2
- 239000000049 pigment Substances 0.000 description 2
- 235000013599 spices Nutrition 0.000 description 2
- 239000003643 water by type Substances 0.000 description 2
- 235000005744 Bacillus subtilis subsp subtilis Nutrition 0.000 description 1
- 241000948854 Bacillus subtilis subsp. subtilis Species 0.000 description 1
- 241000195649 Chlorella <Chlorellales> Species 0.000 description 1
- 244000086443 Craterellus fallax Species 0.000 description 1
- 235000007926 Craterellus fallax Nutrition 0.000 description 1
- 108090000790 Enzymes Proteins 0.000 description 1
- 102000004190 Enzymes Human genes 0.000 description 1
- 244000068988 Glycine max Species 0.000 description 1
- 235000010469 Glycine max Nutrition 0.000 description 1
- KDXKERNSBIXSRK-UHFFFAOYSA-N Lysine Natural products NCCCCC(N)C(O)=O KDXKERNSBIXSRK-UHFFFAOYSA-N 0.000 description 1
- 239000004472 Lysine Substances 0.000 description 1
- OVBPIULPVIDEAO-UHFFFAOYSA-N N-Pteroyl-L-glutaminsaeure Natural products C=1N=C2NC(N)=NC(=O)C2=NC=1CNC1=CC=C(C(=O)NC(CCC(O)=O)C(O)=O)C=C1 OVBPIULPVIDEAO-UHFFFAOYSA-N 0.000 description 1
- 229910002651 NO3 Inorganic materials 0.000 description 1
- NHNBFGGVMKEFGY-UHFFFAOYSA-N Nitrate Chemical compound [O-][N+]([O-])=O NHNBFGGVMKEFGY-UHFFFAOYSA-N 0.000 description 1
- KEJOCWOXCDWNID-UHFFFAOYSA-N Nitrilooxonium Chemical class [O+]#N KEJOCWOXCDWNID-UHFFFAOYSA-N 0.000 description 1
- JVMRPSJZNHXORP-UHFFFAOYSA-N ON=O.ON=O.ON=O.N Chemical compound ON=O.ON=O.ON=O.N JVMRPSJZNHXORP-UHFFFAOYSA-N 0.000 description 1
- OAICVXFJPJFONN-UHFFFAOYSA-N Phosphorus Chemical compound [P] OAICVXFJPJFONN-UHFFFAOYSA-N 0.000 description 1
- 241000420927 Pseudomonas xanthomarina Species 0.000 description 1
- 241000487245 Rhodococcus yunnanensis Species 0.000 description 1
- 240000004808 Saccharomyces cerevisiae Species 0.000 description 1
- UCKMPCXJQFINFW-UHFFFAOYSA-N Sulphide Chemical compound [S-2] UCKMPCXJQFINFW-UHFFFAOYSA-N 0.000 description 1
- XSQUKJJJFZCRTK-UHFFFAOYSA-N Urea Chemical compound NC(N)=O XSQUKJJJFZCRTK-UHFFFAOYSA-N 0.000 description 1
- 238000009825 accumulation Methods 0.000 description 1
- 239000003463 adsorbent Substances 0.000 description 1
- 238000013019 agitation Methods 0.000 description 1
- PNEYBMLMFCGWSK-UHFFFAOYSA-N aluminium oxide Inorganic materials [O-2].[O-2].[O-2].[Al+3].[Al+3] PNEYBMLMFCGWSK-UHFFFAOYSA-N 0.000 description 1
- 150000003863 ammonium salts Chemical class 0.000 description 1
- 238000004458 analytical method Methods 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 230000008901 benefit Effects 0.000 description 1
- 229960002685 biotin Drugs 0.000 description 1
- 235000020958 biotin Nutrition 0.000 description 1
- 239000011616 biotin Substances 0.000 description 1
- 210000000476 body water Anatomy 0.000 description 1
- 239000004202 carbamide Substances 0.000 description 1
- 235000021466 carotenoid Nutrition 0.000 description 1
- 150000001747 carotenoids Chemical class 0.000 description 1
- 230000015556 catabolic process Effects 0.000 description 1
- 230000008859 change Effects 0.000 description 1
- 229930002875 chlorophyll Natural products 0.000 description 1
- 235000019804 chlorophyll Nutrition 0.000 description 1
- 239000001752 chlorophylls and chlorophyllins Substances 0.000 description 1
- 239000005515 coenzyme Substances 0.000 description 1
- 229910052593 corundum Inorganic materials 0.000 description 1
- 230000000850 deacetylating effect Effects 0.000 description 1
- 238000006731 degradation reaction Methods 0.000 description 1
- 238000013461 design Methods 0.000 description 1
- 238000004090 dissolution Methods 0.000 description 1
- 229940079593 drug Drugs 0.000 description 1
- 230000007613 environmental effect Effects 0.000 description 1
- 238000002474 experimental method Methods 0.000 description 1
- 238000000605 extraction Methods 0.000 description 1
- 239000000796 flavoring agent Substances 0.000 description 1
- 235000019634 flavors Nutrition 0.000 description 1
- 229960000304 folic acid Drugs 0.000 description 1
- 235000019152 folic acid Nutrition 0.000 description 1
- 239000011724 folic acid Substances 0.000 description 1
- 239000010794 food waste Substances 0.000 description 1
- 239000013505 freshwater Substances 0.000 description 1
- 238000000227 grinding Methods 0.000 description 1
- 230000007773 growth pattern Effects 0.000 description 1
- JEGUKCSWCFPDGT-UHFFFAOYSA-N h2o hydrate Chemical compound O.O JEGUKCSWCFPDGT-UHFFFAOYSA-N 0.000 description 1
- 239000000852 hydrogen donor Substances 0.000 description 1
- 230000003834 intracellular effect Effects 0.000 description 1
- 238000004519 manufacturing process Methods 0.000 description 1
- 238000005259 measurement Methods 0.000 description 1
- 235000013372 meat Nutrition 0.000 description 1
- 239000002207 metabolite Substances 0.000 description 1
- 230000000813 microbial effect Effects 0.000 description 1
- CUXQLKLUPGTTKL-UHFFFAOYSA-M microcosmic salt Chemical compound [NH4+].[Na+].OP([O-])([O-])=O CUXQLKLUPGTTKL-UHFFFAOYSA-M 0.000 description 1
- 238000002156 mixing Methods 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 229930014626 natural product Natural products 0.000 description 1
- 231100000252 nontoxic Toxicity 0.000 description 1
- 230000003000 nontoxic effect Effects 0.000 description 1
- 235000008935 nutritious Nutrition 0.000 description 1
- 238000007254 oxidation reaction Methods 0.000 description 1
- 230000010627 oxidative phosphorylation Effects 0.000 description 1
- 229910052698 phosphorus Inorganic materials 0.000 description 1
- 239000011574 phosphorus Substances 0.000 description 1
- 230000003567 photophosphorylation Effects 0.000 description 1
- 229910052573 porcelain Inorganic materials 0.000 description 1
- 244000144977 poultry Species 0.000 description 1
- 244000038651 primary producers Species 0.000 description 1
- 230000001681 protective effect Effects 0.000 description 1
- 230000000630 rising effect Effects 0.000 description 1
- 230000009919 sequestration Effects 0.000 description 1
- 238000007711 solidification Methods 0.000 description 1
- 230000008023 solidification Effects 0.000 description 1
- 238000001179 sorption measurement Methods 0.000 description 1
- 241000894007 species Species 0.000 description 1
- 238000003756 stirring Methods 0.000 description 1
- 239000000126 substance Substances 0.000 description 1
- 230000001225 therapeutic effect Effects 0.000 description 1
- 231100000331 toxic Toxicity 0.000 description 1
- 230000002588 toxic effect Effects 0.000 description 1
- 238000012549 training Methods 0.000 description 1
- 238000003911 water pollution Methods 0.000 description 1
- 229910001845 yogo sapphire Inorganic materials 0.000 description 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F3/00—Biological treatment of water, waste water, or sewage
- C02F3/34—Biological treatment of water, waste water, or sewage characterised by the microorganisms used
-
- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F1/00—Treatment of water, waste water, or sewage
- C02F1/28—Treatment of water, waste water, or sewage by sorption
- C02F1/286—Treatment of water, waste water, or sewage by sorption using natural organic sorbents or derivatives thereof
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/20—Bacteria; Culture media therefor
-
- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F2103/00—Nature of the water, waste water, sewage or sludge to be treated
- C02F2103/20—Nature of the water, waste water, sewage or sludge to be treated from animal husbandry
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Engineering & Computer Science (AREA)
- Organic Chemistry (AREA)
- Microbiology (AREA)
- Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Environmental & Geological Engineering (AREA)
- Biotechnology (AREA)
- Water Supply & Treatment (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Hydrology & Water Resources (AREA)
- Medicinal Chemistry (AREA)
- Biomedical Technology (AREA)
- Virology (AREA)
- Tropical Medicine & Parasitology (AREA)
- Biochemistry (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Biodiversity & Conservation Biology (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
Abstract
The invention discloses a kind of water body purification of aquaculture agent and preparation method thereof.The present invention is by the way that photosynthetic bacteria, denitrifying bacteria, bacillus are cultivated in the culture medium containing porous SiC ceramics particulate so that microorganism is enriched with porous SiC ceramics particulate and a kind of water body purification of aquaculture agent is prepared.Compared with prior art, the water body purification of aquaculture agent that prepared by the present invention possesses good effect to the purification of water quality in breeding water body, prevention from suffering from the diseases etc..
Description
Technical field
The present invention relates to microorganism water purification treatment technology, more particularly to a kind of water body purification of aquaculture technology.
Background technology
In aquaculture system, waters microecosystem be by pathogenic microorganism, opportunist and it is non-cause a disease it is micro-
Biotic component, these microorganisms cooperate with each other each other, in metastable dynamic balance state.But cultivation is highly dense
The addition of degree and artitificial food so that Aquaculture ecosystem turns into a unstable ecosystem, the ripple of any one link
It is dynamic all to cause disruption of ecological balance.Balance is once broken, then and harmful bacteria will be converted into pathogenic bacteria according to incompletely statistics,
2005~20015, China's aquiculture disease average year was lost up to 10,000,000,000 yuan.The control of China aquiculture disease with
It is not high plus poultry feeders culture and specialized capability along with technology is relatively single based on medical treatment, blindly used in production
Medicine, so as to cause the repeated contamination of water body, on the other hand largely and frequently pathogenic microorganism is easily set to produce anti-medicine using medicine
Property, so as to lose therapeutic effect.
The development of culture fishery, aquaculture specific yield increase substantially, fishes and shrimps excreta, food waste and medicine
Abuse causes severe water pollution, particularly raises the later stage, has a strong impact on the growth of aquatic environment and fish.
Photosynthetic bacteria has different physiological roles, and metabolic type is extremely various, there is photoautotrophy, heterotrophism and a variety of battalion of mixed breeding
Growth pattern is supported, there is photosynthetic, carbon sequestration, degraded larger molecular organicses, fixed nitrogen, denitrogenation, nitrification, denitrification, sulfide-oxidation etc.
Metabolic way.Photosynthetic bacteria is primary producer important in waters, is being sick of with illumination condition, with a variety of sulfide or is having
Machine thing makees hydrogen donor and fixes progress photophosphorylation and photoredox reaction；Under aerobic dark condition, the conjunction of photosynthetic pigments
Into being suppressed, intracellular lacks endoplasm membranous system, and energy is obtained by oxidative phosphorylation, can also be obtained in addition by denitrogenation or fermentation
Obtain energy.Utilization of the photosynthetic bacteria to nitrogen source is quite varied, using ammonium salt, ammonia nitrogen, minority kind can also utilize nitrate and
Urea.This characteristic for neatly changing metabolic type with the change of growth conditions together form with other photosynthetic organisms
The original producer of the nature ecosystem, played an important role in the conversion of nature material and energy circulation.
The thalline of photosynthetic bacteria is nontoxic, nutritious, and protein content exceedes, more than chlorella and soybean, and amino
Acid composition is complete, and containing amino acid is planted necessary to organism, amino acid composition is very reasonable, in addition to the amount containing amino acid is less,
Other kinds amino acid content is all many, and especially lysine content is compared with horn of plenty.Photosynthetic bacteria is also containing abundant race simultaneously
Vitamin, especially vitamin, folic acid, biotin equal size are at a relatively high, and the vitamin species being practically free of in yeast is in
Content is more.Coenzyme content in photosynthetic bacteria body also substantially exceeds other biological, in addition, photosynthetic bacteria is rich in substantial amounts of bacterium
Chlorophylls and Carotenoids, it is the outstanding source of natural pigment.
At present, high-density breeding can cause to accumulate substantial amounts of residual bait, excrement and other metabolins in pond, and these materials are in pond
It is corrupt in the pool, a large amount of harmful substances are produced, cause microorganism amount reproduction to include pathogenic bacteria, dissolved oxygen declines, value rising and three
State nitrogen rises, especially nitrite nitrogen.The excess accumulation of nitrosonium salts has toxic action to aquaculture organism, and high concentration nitrite can
The death such as fishes and shrimps are caused, low concentration nitrite influences the activity of fish enzyme, causes fishes and shrimps etc. slow-growing.Therefore, in order to purify
Breeding water body water quality, development cost is low, ecological, environmental protective, free of contamination water body purification of aquaculture agent are very necessary.
The content of the invention
In view of the drawbacks described above of prior art, the technical problems to be solved by the invention are to utilize photosynthetic bacteria, denitrification
The materials such as bacterium, bacillus, adsorbent prepare a kind of water body purification of aquaculture agent.
A kind of water body purification of aquaculture agent, it is characterised in that be prepared by the raw material of following weight parts：Photosynthetic bacteria
40~50 parts of agent, 10~20 parts of denitrifying bacteria agent, 10~20 parts of bacillus agent, 10~20 parts of moringa seeds extract solution and 4~6
Part Chitosan/Cellulose complex microsphere.
Preferably, the preparation method of described photosynthetic bacteria agent is as follows：
A), prepared by seed liquor
On sterile operating desk, photosynthetic bacteria is inoculated in seed culture medium, at 28~33 DEG C, 180rpm/min shakes
Thalline is cultivated in bed under illumination to cell concentration up to 108~109CFU/mL, obtain photosynthetic bacteria seed liquor；
B), fermented and cultured
5mL photosynthetic bacteria seed liquors are added in fermentation medium, the illumination in 28~33 DEG C, 180rpm/min shaking tables
Lower culture, thalline quantity in zymophyte is detected, treats that thalline quantity reaches 1010Stop fermentation during CFU/mL, obtain photosynthetic bacteria agent.
Preferably, described denitrifying bacteria agent, the preparation method of bacillus agent are as follows：
A), prepared by seed liquor
On sterile operating desk, denitrifying bacteria or bacillus are inoculated in seed culture medium, 28~33
DEG C, thalline is cultivated in 180rpm/min shaking tables to cell concentration up to 108~109CFU/mL, obtain denitrifying bacteria seed liquor or
Bacillus seed liquor；
B), fermented and cultured
The above-mentioned seed liquors of 5mL are added in fermentation medium, cultivated in 28~33 DEG C, 180rpm/min shaking tables, are examined
Thalline quantity in zymophyte is surveyed, treats that thalline quantity reaches 1010Stop fermentation during CFU/mL, obtain denitrifying bacteria agent or gemma
Bacillus agent.
Preferably, photosynthetic bacteria agent, denitrifying bacteria agent, the pH of seed culture medium described in bacillus agent for 7.0~
7.2, the material containing following percentage by weight：1~3 part of ammonium chloride, 4~6 parts of sodium acetate, 0.1 part of magnesium chloride, calcium chloride 0.1
Part, 1 part of potassium dihydrogen phosphate, 0.5 part of dipotassium hydrogen phosphate, 0.1 part of yeast extract, 1000 parts of water.
Preferably, photosynthetic bacteria agent, denitrifying bacteria agent, the pH of fermentation medium described in bacillus agent for 7.0~
7.2, the material containing following percentage by weight：8~10 parts of porous SiC ceramics particulate, 4~6 parts of beef extract, peptone 8~12
Part, 5 parts of dipotassium hydrogen phosphate, 2 parts of sodium chloride, 1000 parts of water.
Addition porous SiC ceramics particulate can make microorganism fungus kind largely be attached to porous SiC pottery in the fermentation medium
On porcelain particulate.After water purification agent is put into aquaculture system, the porous SiC ceramics particulate for being attached with a large amount of microorganisms is deposited to
Pond is low, forms strain advantage in bed mud rapidly, the ammonia and nitrogen organic in bed mud, nitrite etc. is carried out biodegradable.
Preferably, described photosynthetic bacteria is Rhodopseudomonas palustris CGMCC 1.2180, Rhodospirillum rubrum CGMCC
1.3369th, one kind in Yunnan Rhodococcus sp CGMCC 4.3558.
Preferably, described denitrifying bacteria is yellow sea pseudomonad CGMCC 1.8020 or Pseudomonas stutzeri
CGMCC 1.8597。
Preferably, described bacillus is bacillus subtilis CGMCC 1.504, bacillus licheniformis CGMCC
1.105th, one kind in Bacillus coagulans CGMCC 1.10302, bacillus pumilus CGMCC 1.8167.
It is highly preferred that the photosynthetic bacteria is Rhodopseudomonas palustris CGMCC 1.2180, Rhodospirillum rubrum CGMCC
1.3369th, Yunnan Rhodococcus sp CGMCC 4.3558 in mass ratio 1:1:The mixture of 1 composition.
Preferably, the preparation method of described porous SiC ceramics particulate is as follows：
A. SiC powder, Al are taken2O3Powder and graphite are added in ethanol, are uniformly mixed, and base substrate is pressed into after drying；
B. base substrate in step a is put into Muffle furnace and calcined, be cooled to room temperature and obtain porous SiC ceramics piece；
C. the porous SiC ceramics piece that step b is obtained is crushed with pulverizer, obtains porous SiC ceramics particulate.
A kind of preparation method of water body purification of aquaculture agent, it is characterised in that：By 40~50 parts by weight photosynthetic bacteria agent,
10~20 parts by weight denitrifying bacteria agent, 10~20 parts by weight bacillus agent, 10~20 parts by weight moringa seeds extract solutions and 4~
6 parts by weight Chitosan/Cellulose complex microspheres produce after being stirred in sterile barrel.
Preferably, the preparation method of the chitosan/vitamin complex microsphere is as follows：
Take cellulose and chitosan to be dissolved in respectively in ionic liquid 1- ethyl-3-methylimidazole acetate, obtain transparent
Cellulose solution and chitosan solution, the cellulose solution after dissolving and chitosan solution are mixed, heated under 100 DEG C of oil baths
1~3h of crosslinking becomes faint yellow homogeneous liquid to mixed liquor, and obtained faint yellow homogeneous liquid is loaded into injector for medical purpose
In, mixed liquor is pushed into absolute ethyl alcohol coagulating bath and obtains gel state composite pellets；The gel state composite pellets being prepared exist
Continue to soak 24h in coagulating bath, finally cleaned with deionized water, obtain Chitosan/Cellulose complex microsphere.
Preferably, the preparation method of the moringa seeds extract solution is as follows：
The unabroken moringa seeds 5g in surface is chosen, be added to after crushing in 500mL 1mol/L sodium chloride solution 40~
30~60min is stirred at 60 DEG C under water bath with thermostatic control, with the glass fibre membrane filtration that aperture is 0.5 μM, obtained filtrate is as peppery
Wooden seed extract solution.
Addition possesses the moringa seeds extract solution of adsorption capacity in cleanser and Chitosan/Cellulose complex microsphere can be very well
The materials such as the organic matter to be suspended in aquaculture system, excreta are rapidly settled down to pond is low, for the microorganism in bed mud
Decompose.Moreover, the transparency increase of water body so that photosynthetic bacteria existing for script can sufficiently utilize luminous energy in bed mud,
Accelerate to accelerate to the ammonia and nitrogen organic in water body, the degraded of nitrite while breeding.
Beneficial effects of the present invention：
1st, a kind of water body purification of aquaculture agent of the present invention can significantly reduce breeding water body nitrite, ammonia nitrogen,
The content of active phosphorus；The content of oxygen in water is obviously improved simultaneously, adjusts the pH of water body；So that the water quality of aquaculture system
It is greatly improved.
2nd, the water quality of notable aquaculture system is capable of in a kind of water body purification of aquaculture agent of the present invention so that aquatic products
Survival rate is greatly improved.Moreover, the abundant metabolite of microbial cells so that aquatic products body weight speedup, meat
Matter is also greatly improved with flavor.
3rd, instant invention overcomes prior art prejudice, the red bacterium of fixed nitrogen has been carried out under conditions of no light to expand training
Support, the photosynthetic bacteria agent being prepared possesses the characteristic of good degradation of ammonia nitrogen organic matter and nitrite.
Embodiment
Each raw material sources in embodiment：
Rhodopseudomonas palustris：Rhodopseudomonas palustris, CGMCC 1.2180, it is purchased from Chinese common micro-
Biological inoculum preservation administrative center；
Rhodospirillum rubrum：Rhodospirillum rubrum, CGMCC 1.3369, is purchased from China General Microbiological strain
Preservation administrative center；
Yunnan Rhodococcus sp：Rhodococcus yunnanensis, CGMCC 4.3558, is purchased from China General Microbiological bacterium
Kind preservation administrative center；
Yellow sea pseudomonad：Pseudomonas xanthomarina, CGMCC bacterium numberings：1.8020 it is purchased from China
General Microbiological Culture preservation administrative center；
Pseudomonas stutzeri：Pseudomonas stutzeri, CGMCC bacterium numberings：1.8597, it is purchased from Chinese common micro-
Biological inoculum preservation administrative center；
Bacillus subtilis：Bacillus subtilis subsp.Subtilis, CGMCC bacterium numberings：1.504 purchase
In China General Microbiological culture presevation administrative center；
Bacillus licheniformis：Bacillus licheniformis, CGMCC bacterium numberings：1.105, it is purchased from Chinese common
Microbiological Culture Collection administrative center；
Bacillus coagulans：Bacillus coagulans, CGMCC bacterium numberings：1.10302 it is purchased from Chinese common micro-
Biological inoculum preservation administrative center；
Bacillus pumilus：Bacillus pumilus, CGMCC bacterium numberings：1.8167 it is purchased from China General Microbiological
Culture presevation administrative center；
Moringa seeds：Yunnan carat draws agricultural science and technology Co., Ltd；
Chitosan：No. CAS：9012-76-4, deacetylating degree of chitosan 95%, Shaanxi Sen Fu natural products Co., Ltd；
Cellulose：No. CAS：9004-34-6, average grain diameter：250 μM, Shanghai Aladdin biochemical technology limited company；
1- ethyl-3-methylimidazole acetate：No. CAS：143314-17-4, content >=99%, weak yellow liquid, water contain
Amount≤2000ppm, Chenzhou Ke Neng materials Science and Technology Ltd.；
Moringa seeds extract solution in embodiment is prepared by the following method：
The unabroken moringa seeds in surface are chosen, are crushed with Shanghai plum perfume (or spice) XA-1 pulverizers, setting rotating speed is 2000rpm/
Min, grinding time 3min.The moringa seeds powder after 5g crushing is taken to be added in 500mL 1mol/L sodium chloride solution at 50 DEG C
30min is stirred under lower water bath with thermostatic control, with the glass fibre membrane filtration that aperture is 0.5 μM, obtained filtrate is moringa seeds extraction
Liquid.
Chitosan/Cellulose complex microsphere in embodiment is prepared by the following method：
5g celluloses and 5g chitosans is taken to be dissolved in respectively in 100mL ionic liquid 1- ethyl-3-methylimidazole acetate,
It is completely dissolved at 70 DEG C in water bath with thermostatic control, uniform magnetic agitation is kept in course of dissolution, it is molten to finally give transparency cellulose
Liquid and chitosan solution.The cellulose after dissolving and chitosan solution are mixed again, heat cross-linking 1h is to mixed under 100 DEG C of oil baths
Close liquid and become faint yellow homogeneous liquid.Obtained weak yellow liquid is fitted into injector for medical purpose, mixed liquor is pushed into anhydrous
Gel state composite pellets are obtained in alcohol solidification bath；The gel state composite pellets being prepared continue to soak 24h in coagulating bath,
Finally cleaned 3 times with deionized water, obtain Chitosan/Cellulose complex microsphere.
Porous SiC ceramics particulate in embodiment is prepared by the following method：
Take 70g SiC, 30gAl2O3It is added to 20g graphite in 100mL ethanol, 24h is stirred in ball grinder, mixing is equal
30min is dried after even at 100 DEG C, crosses 50 mesh sieves.Unidirectional pressurization 30MPa is pressed into 10mm × 5mm × 5mm rectangular strip base
Body.Above-mentioned base substrate is put into thermostatic drying chamber, dries 24h at 110 DEG C.It is cooled to after room temperature and above-mentioned base substrate is put into Muffle furnace
In, 900 DEG C, 5 DEG C/min of heating rate of first paragraph reaction temperature is set, reaches constant temperature 2h after setting temperature, second segment reaction temperature
1500 DEG C, 5 DEG C/min of heating rate of degree, constant temperature 3h after setting temperature is reached,.Finally, it is cooled to room temperature and obtains porous SiC ceramics
Piece.
Above-mentioned porous SiC ceramics piece is smashed with Shanghai plum perfume (or spice) XA-1 pulverizers, setting rotating speed is 2000rpm/min, powder
The broken time is 3min, obtains porous SiC ceramics particulate.
SiC powder：Content >=97%, 1~3mm of granularity, Gongyi City surmount filtrate factory；
Al2O3Powder：Content >=98.5%, 10~15 μm of granularity, great river grinding-material Co., Ltd；
Graphite：Fixed carbon >=99.8%, 0.3~3mm of granularity, Linzhang County Jin Wang carbons Co., Ltd；
Embodiment 1
A kind of preparation method of photosynthetic bacteria agent is as follows：
A), prepared by seed liquor
On sterile operating desk, Rhodopseudomonas palustris is inoculated in seed culture medium, in 30 DEG C, 180rpm/min
In shaking table, illumination cultivation thalline is to cell concentration up to 10 under 2400lux incandescent lamp8CFU/mL, obtain Rhodopseudomonas palustris
Seed liquor；
B), fermented and cultured
5mL Rhodopseudomonas palustris seed liquors are added in fermentation medium, in 30 DEG C, 180rpm/min shaking tables,
Illumination cultivation under 2400lux incandescent lamp, thalline quantity in zymophyte is detected, treats that thalline quantity reaches 1010Stop during CFU/mL
Fermentation, obtains photosynthetic bacteria agent.
The pH of described seed culture medium is 7.0, contains following material：Ammonium chloride 2g, sodium acetate 5g, magnesium chloride 0.1g,
Calcium chloride 0.1g, potassium dihydrogen phosphate 1g, dipotassium hydrogen phosphate 0.5g, yeast extract 0.1g, water 1000g.
The pH of described fermentation medium is 7.0, contains following material：Porous SiC ceramics particulate 9g, beef extract 5g, egg
White peptone 10g, dipotassium hydrogen phosphate 5g, sodium chloride 2g, water 1000g.
Embodiment 2
A kind of preparation method of photosynthetic bacteria agent is as follows：
A), prepared by seed liquor
On sterile operating desk, Rhodospirillum rubrum is inoculated in seed culture medium, in 30 DEG C, 180rpm/min shaking tables
In, illumination cultivation thalline is to cell concentration up to 10 under 2400lux incandescent lamp8CFU/mL, obtain Rhodospirillum rubrum seed liquor；
B), fermented and cultured
5mL Rhodospirillum rubrum seed liquors are added in fermentation medium, in 30 DEG C, 180rpm/min shaking tables,
Illumination cultivation under 2400lux incandescent lamp, thalline quantity in zymophyte is detected, treats that thalline quantity reaches 1010Stop during CFU/mL
Fermentation, obtains photosynthetic bacteria agent.
The pH of described seed culture medium is 7.0, contains following material：Ammonium chloride 2g, sodium acetate 5g, magnesium chloride 0.1g,
Calcium chloride 0.1g, potassium dihydrogen phosphate 1g, dipotassium hydrogen phosphate 0.5g, yeast extract 0.1g, water 1000g.
The pH of described fermentation medium is 7.0, contains following material：Porous SiC ceramics particulate 9g, beef extract 5g, egg
White peptone 10g, dipotassium hydrogen phosphate 5g, sodium chloride 2g, water 1000g.
Embodiment 3
A kind of preparation method of photosynthetic bacteria agent is as follows：
A), prepared by seed liquor
On sterile operating desk, Yunnan Rhodococcus sp is inoculated in seed culture medium, in 30 DEG C, 180rpm/min shaking tables
In, illumination cultivation thalline is to cell concentration up to 10 under 2400lux incandescent lamp8CFU/mL, obtain Yunnan Rhodococcus sp seed liquor；
B), fermented and cultured
5mL Yunnan Rhodococcus sp seed liquor is added in fermentation medium, in 30 DEG C, 180rpm/min shaking tables,
Illumination cultivation under 2400lux incandescent lamp, thalline quantity in zymophyte is detected, treats that thalline quantity reaches 1010Stop during CFU/mL
Fermentation, obtains photosynthetic bacteria agent.
The pH of described seed culture medium is 7.0, contains following material：Ammonium chloride 2g, sodium acetate 5g, magnesium chloride 0.1g,
Calcium chloride 0.1g, potassium dihydrogen phosphate 1g, dipotassium hydrogen phosphate 0.5g, yeast extract 0.1g, water 1000g.
The pH of described fermentation medium is 7.0, contains following material：Porous SiC ceramics particulate 9g, beef extract 5g, egg
White peptone 10g, dipotassium hydrogen phosphate 5g, sodium chloride 2g, water 1000g.
Embodiment 4
A kind of preparation method of denitrifying bacteria agent is as follows：
A), prepared by seed liquor
On sterile operating desk, yellow sea pseudomonad is inoculated in seed culture medium, in 30 DEG C, 180rpm/min
Thalline is cultivated in shaking table to cell concentration up to 108CFU/mL, obtain yellow sea pseudomonad seed liquor；
B), fermented and cultured
5mL yellow sea pseudomonad seed liquor is added in fermentation medium, trained in 30 DEG C, 180rpm/min shaking tables
Support, detect thalline quantity in zymophyte, treat that thalline quantity reaches 1010Stop fermentation during CFU/mL, obtain denitrifying bacteria agent.
The pH of described seed culture medium is 7.0, contains following material：Ammonium chloride 2g, sodium acetate 5g, magnesium chloride 0.1g,
Calcium chloride 0.1g, potassium dihydrogen phosphate 1g, dipotassium hydrogen phosphate 0.5g, yeast extract 0.1g, water 1000g.
The pH of described fermentation medium is 7.0, contains following material：Porous SiC ceramics particulate 9g, beef extract 5g, egg
White peptone 10g, dipotassium hydrogen phosphate 5g, sodium chloride 2g, water 1000g.
Embodiment 5
A kind of preparation method of bacillus agent is as follows：
A), prepared by seed liquor
On sterile operating desk, bacillus subtilis is inoculated in seed culture medium, shaken in 30 DEG C, 180rpm/min
Thalline is cultivated in bed to cell concentration up to 108CFU/mL, obtain bacillus subtilis seed liquor；
B), fermented and cultured
5mL bacillus subtilis seed liquors are added in fermentation medium, trained in 30 DEG C, 180rpm/min shaking tables
Support, detect thalline quantity in zymophyte, treat that thalline quantity reaches 1010Stop fermentation during CFU/mL, obtain bacillus agent.
The pH of described seed culture medium is 7.0, contains following material：Ammonium chloride 2g, sodium acetate 5g, magnesium chloride 0.1g,
Calcium chloride 0.1g, potassium dihydrogen phosphate 1g, dipotassium hydrogen phosphate 0.5g, yeast extract 0.1g, water 1000g.
The pH of described fermentation medium is 7.0, contains following material：Porous SiC ceramics particulate 9g, beef extract 5g, egg
White peptone 10g, dipotassium hydrogen phosphate 5g, sodium chloride 2g, water 1000g.
Embodiment 6
A kind of preparation method of photosynthetic bacteria agent is as follows：
A), prepared by seed liquor
On sterile operating desk, Rhodopseudomonas palustris is inoculated in seed culture medium, in 30 DEG C, 180rpm/min
In shaking table, illumination cultivation thalline is to cell concentration up to 10 under 2400lux incandescent lamp8CFU/mL, obtain Rhodopseudomonas palustris
Seed liquor；
B), fermented and cultured
5mL Rhodopseudomonas palustris seed liquors are added in fermentation medium, in 30 DEG C, 180rpm/min shaking tables,
Illumination cultivation under 2400lux incandescent lamp, thalline quantity in zymophyte is detected, treats that thalline quantity reaches 1010Stop during CFU/mL
Fermentation, obtains photosynthetic bacteria agent.
The pH of described seed culture medium is 7.0, contains following material：Ammonium chloride 2g, sodium acetate 5g, magnesium chloride 0.1g,
Calcium chloride 0.1g, potassium dihydrogen phosphate 1g, dipotassium hydrogen phosphate 0.5g, yeast extract 0.1g, water 1000g.
The pH of described fermentation medium is 7.0, contains following material：Beef extract 5g, peptone 10g, dipotassium hydrogen phosphate
5g, sodium chloride 2g, water 1000g.
Embodiment 7
A kind of preparation method of photosynthetic bacteria agent is as follows：
A), prepared by seed liquor
On sterile operating desk, Rhodospirillum rubrum is inoculated in seed culture medium, in 30 DEG C, 180rpm/min shaking tables
In, illumination cultivation thalline is to cell concentration up to 10 under 2400lux incandescent lamp8CFU/mL, obtain Rhodospirillum rubrum seed liquor；
B), fermented and cultured
5mL Rhodospirillum rubrum seed liquors are added in fermentation medium, in 30 DEG C, 180rpm/min shaking tables,
Illumination cultivation under 2400lux incandescent lamp, thalline quantity in zymophyte is detected, treats that thalline quantity reaches 1010Stop during CFU/mL
Fermentation, obtains photosynthetic bacteria agent.
The pH of described seed culture medium is 7.0, contains following material：Ammonium chloride 2g, sodium acetate 5g, magnesium chloride 0.1g,
Calcium chloride 0.1g, potassium dihydrogen phosphate 1g, dipotassium hydrogen phosphate 0.5g, yeast extract 0.1g, water 1000g.
The pH of described fermentation medium is 7.0, contains following material：Beef extract 5g, peptone 10g, dipotassium hydrogen phosphate
5g, sodium chloride 2g, water 1000g.
Embodiment 8
A kind of preparation method of photosynthetic bacteria agent is as follows：
A), prepared by seed liquor
On sterile operating desk, Yunnan Rhodococcus sp is inoculated in seed culture medium, in 30 DEG C, 180rpm/min shaking tables
In, illumination cultivation thalline is to cell concentration up to 10 under 2400lux incandescent lamp8CFU/mL, obtain Yunnan Rhodococcus sp seed liquor；
B), fermented and cultured
5mL Yunnan Rhodococcus sp seed liquor is added in fermentation medium, in 30 DEG C, 180rpm/min shaking tables,
Illumination cultivation under 2400lux incandescent lamp, thalline quantity in zymophyte is detected, treats that thalline quantity reaches 1010Stop during CFU/mL
Fermentation, obtains photosynthetic bacteria agent.
The pH of described seed culture medium is 7.0, contains following material：Ammonium chloride 2g, sodium acetate 5g, magnesium chloride 0.1g,
Calcium chloride 0.1g, potassium dihydrogen phosphate 1g, dipotassium hydrogen phosphate 0.5g, yeast extract 0.1g, water 1000g.
The pH of described fermentation medium is 7.0, contains following material：Beef extract 5g, peptone 10g, dipotassium hydrogen phosphate
5g, sodium chloride 2g, water 1000g.
Embodiment 9
A kind of preparation method of denitrifying bacteria agent is as follows：
A), prepared by seed liquor
On sterile operating desk, yellow sea pseudomonad is inoculated in seed culture medium, in 30 DEG C, 180rpm/min
Thalline is cultivated in shaking table to cell concentration up to 108CFU/mL, obtain yellow sea pseudomonad seed liquor；
B), fermented and cultured
5mL yellow sea pseudomonad seed liquor is added in fermentation medium, trained in 30 DEG C, 180rpm/min shaking tables
Support, detect thalline quantity in zymophyte, treat that thalline quantity reaches 1010Stop fermentation during CFU/mL, obtain denitrifying bacteria agent.
The pH of described seed culture medium is 7.0, contains following material：Ammonium chloride 2g, sodium acetate 5g, magnesium chloride 0.1g,
Calcium chloride 0.1g, potassium dihydrogen phosphate 1g, dipotassium hydrogen phosphate 0.5g, yeast extract 0.1g, water 1000g.
The pH of described fermentation medium is 7.0, contains following material：Beef extract 5g, peptone 10g, dipotassium hydrogen phosphate
5g, sodium chloride 2g, water 1000g.
Embodiment 10
A kind of preparation method of bacillus agent is as follows：
A), prepared by seed liquor
On sterile operating desk, bacillus subtilis is inoculated in seed culture medium, shaken in 30 DEG C, 180rpm/min
Thalline is cultivated in bed to cell concentration up to 108CFU/mL, obtain bacillus subtilis seed liquor；
B), fermented and cultured
5mL bacillus subtilis seed liquors are added in fermentation medium, trained in 30 DEG C, 180rpm/min shaking tables
Support, detect thalline quantity in zymophyte, treat that thalline quantity reaches 1010Stop fermentation during CFU/mL, obtain bacillus agent.
The pH of described seed culture medium is 7.0, contains following material：Ammonium chloride 2g, sodium acetate 5g, magnesium chloride 0.1g,
Calcium chloride 0.1g, potassium dihydrogen phosphate 1g, dipotassium hydrogen phosphate 0.5g, yeast extract 0.1g, water 1000g.
The pH of described fermentation medium is 7.0, contains following material：Beef extract 5g, peptone 10g, dipotassium hydrogen phosphate
5g, sodium chloride 2g, water 1000g.
Embodiment 11
A kind of preparation method of water body purification of aquaculture agent：
By 40 parts by weight photosynthetic bacteria agent, 20 parts by weight denitrifying bacteria agent, 20 parts by weight bacillus agent, 10 parts by weight
Moringa seeds extract solution and 5 parts by weight Chitosan/Cellulose complex microspheres produce after being stirred in sterile barrel.
Described photosynthetic bacteria agent is by the photosynthetic bacteria agent in mass ratio 1 that is prepared in embodiment 6, embodiment 7, embodiment 8:
1:1 well mixed forms；Described denitrifying bacteria agent is what is be prepared in embodiment 9；Described spore bacillus agent is implementation
It is prepared in example 10.
Embodiment 12
A kind of preparation method of water body purification of aquaculture agent：
By 40 parts by weight photosynthetic bacteria agent, 20 parts by weight denitrifying bacteria agent, 20 parts by weight bacillus agent in sterile barrel
In stir after produce.
Described photosynthetic bacteria agent is by the photosynthetic bacteria agent in mass ratio 1 that is prepared in embodiment 1, embodiment 2, embodiment 3:
1:1 well mixed forms；Described denitrifying bacteria agent is what is be prepared in embodiment 4；Described spore bacillus agent is implementation
It is prepared in example 5.
Embodiment 13
A kind of preparation method of water body purification of aquaculture agent：
By 40 parts by weight photosynthetic bacteria agent, 20 parts by weight denitrifying bacteria agent, 20 parts by weight bacillus agent, 10 parts by weight
Moringa seeds extract solution and 5 parts by weight Chitosan/Cellulose complex microspheres produce after being stirred in sterile barrel.
Described photosynthetic bacteria agent is by the photosynthetic bacteria agent in mass ratio 1 that is prepared in embodiment 1, embodiment 2, embodiment 3:
1:1 well mixed forms；Described denitrifying bacteria agent is what is be prepared in embodiment 4；Described spore bacillus agent is implementation
It is prepared in example 5.
Embodiment 14
Substantially the same manner as Example 13, it is differed only in：Described photosynthetic bacteria agent is by embodiment 1, embodiment 2
The photosynthetic bacteria agent in mass ratio 1 of preparation:1 well mixed forms.
Embodiment 15
Substantially the same manner as Example 13, it is differed only in：Described photosynthetic bacteria agent is by embodiment 1, embodiment 3
The photosynthetic bacteria agent in mass ratio 1 of preparation:1 well mixed forms.
Embodiment 16
Substantially the same manner as Example 13, it is differed only in：Described photosynthetic bacteria agent is by embodiment 2, embodiment 3
The photosynthetic bacteria agent in mass ratio 1 of preparation:1 well mixed forms.
Test case 1
Take the water purification agent prepared in 2Kg embodiments 11~16 to put into six fishponds respectively and do not launch water purification agent with one
Fishpond compare, the depth of water in seven fishponds is 1.5 meters, and area is one mu, cultivates grass carp before.It is right after 30 days
Fishpond carries out water-quality determination, the foundation of water quality measurement is GB11607-89 National Standard of the People's Republic of China's water quality standard for fishery
With NY5051-2001 pollution-free food aquiculture fresh water water quality, testing index and data are as shown in table 1, table 2.
The water purifier of table 1 is added in fishpond Nitrite after 30 days, ammonia nitrogen, the changes of contents of active microcosmic salt
The water purification agent of table 2 is added in fishpond after 30 days to water body pH, dissolved oxygen influence result
Test case 2
Take the water purification agent prepared in 2Kg embodiments 11~16 to put into six fishponds respectively and do not launch water purification agent with one
Fishpond compare, the depth of water in seven fishponds is 1.5 meters, and area is one mu, cultivates grass carp before.
3 simple photosynthetic bacteria of table is to product body weight survival outcome
| Put into number (tail) | Receive and count (tail) | Survival rate (%) | Average weight (g) | |
| Water purification agent is not launched | 180000 | 119193 | 66.22 | 15.8 |
| Embodiment 11 | 180000 | 125646 | 69.80 | 17.4 |
| Embodiment 12 | 180000 | 129643 | 72.02 | 18.2 |
| Embodiment 13 | 180000 | 155772 | 86.54 | 20.3 |
| Embodiment 14 | 180000 | 128268 | 71.26 | 17.6 |
| Embodiment 15 | 180000 | 128736 | 71.52 | 17.2 |
| Embodiment 16 | 180000 | 126648 | 70.36 | 18.1 |
Preferred embodiment of the invention described in detail above.It should be appreciated that one of ordinary skill in the art without
Creative work can is needed to make many modifications and variations according to the design of the present invention.Therefore, all technologies in the art
Personnel are available by logical analysis, reasoning, or a limited experiment on the basis of existing technology under this invention's idea
Technical scheme, all should be in the protection domain being defined in the patent claims.
Claims (10)
1. a kind of water body purification of aquaculture agent, it is characterised in that be prepared by the raw material of following weight parts：Photosynthetic bacteria agent
40~50 parts, 10~20 parts of denitrifying bacteria agent, 10~20 parts of bacillus agent, 10~20 parts of moringa seeds extract solution and shell gather
4~6 parts of sugar/cellulose composite microsphere.
2. water body purification of aquaculture agent as claimed in claim 1, it is characterised in that：The preparation side of described photosynthetic bacteria agent
Method is as follows：
A), prepared by seed liquor
On sterile operating desk, photosynthetic bacteria is inoculated in seed culture medium, in 28~33 DEG C, 180rpm/min shaking tables
Thalline is cultivated under illumination to cell concentration up to 108~109CFU/mL, obtain photosynthetic bacteria seed liquor；
B), fermented and cultured
5mL photosynthetic bacteria seed liquors are added in fermentation medium, trained in 28~33 DEG C, 180rpm/min shaking tables under illumination
Support, detect thalline quantity in zymophyte, treat that thalline quantity reaches 1010Stop fermentation during CFU/mL, obtain photosynthetic bacteria agent.
3. water body purification of aquaculture agent as claimed in claim 1, it is characterised in that：Described denitrifying bacteria agent, gemma
The preparation method of bacillus agent is as follows：
A), prepared by seed liquor
On sterile operating desk, denitrifying bacteria or bacillus are inoculated in seed culture medium, at 28~33 DEG C,
Thalline is cultivated in 180rpm/min shaking tables to cell concentration up to 108~109CFU/mL, obtain denitrifying bacteria seed liquor or bud
Spore bacillus seed liquor；
B), fermented and cultured
The above-mentioned seed liquors of 5mL are added in fermentation medium, cultivated in 28~33 DEG C, 180rpm/min shaking tables, detection hair
Thalline quantity in yeast-like fungi, treat that thalline quantity reaches 1010Stop fermentation during CFU/mL, obtain denitrifying bacteria agent or bacillus
Agent.
4. the water body purification of aquaculture agent as described in claim 2~3, it is characterised in that：The pH of described seed culture medium
For 7.0~7.2, the material containing following parts by weight：1~3 part of ammonium chloride, 4~6 parts of sodium acetate, 0.1 part of magnesium chloride, calcium chloride
0.1 part, 1 part of potassium dihydrogen phosphate, 0.5 part of dipotassium hydrogen phosphate, 0.1 part of yeast extract, 1000 parts of water.
5. the water body purification of aquaculture agent as described in claim 2~3, it is characterised in that：The pH of described fermentation medium
For 7.0~7.2, the material containing following parts by weight：8~10 parts of porous SiC ceramics particulate, 4~6 parts of beef extract, peptone 8~
12 parts, 5 parts of dipotassium hydrogen phosphate, 2 parts of sodium chloride, 1000 parts of water.
6. the water body purification of aquaculture agent as described in claim 2~3, it is characterised in that：Described photosynthetic bacteria is marsh
One kind in red pseudomonas CGMCC 1.2180, Rhodospirillum rubrum CGMCC 1.3369, Yunnan Rhodococcus sp CGMCC 4.3558.
7. the water body purification of aquaculture agent as described in claim 2~3, it is characterised in that：Described denitrifying bacteria is Huang
Color sea pseudomonad CGMCC 1.8020 or Pseudomonas stutzeri CGMCC 1.8597.
8. water body purification of aquaculture agent as claimed in claim 2, it is characterised in that：Described bacillus is withered grass gemma
Bacillus CGMCC 1.504, bacillus licheniformis CGMCC 1.105, Bacillus coagulans CGMCC 1.10302, short and small gemma bar
One kind in bacterium CGMCC 1.8167.
9. water body purification of aquaculture agent as claimed in claim 5, it is characterised in that：Described porous SiC ceramics particulate
Preparation method is as follows：
A. SiC powder, Al are taken2O3Powder and graphite are added in ethanol, are uniformly mixed, and base substrate is pressed into after drying；
B. base substrate in step a is put into Muffle furnace and calcined, be cooled to room temperature and obtain porous SiC ceramics piece；
C. the porous SiC ceramics piece that step b is obtained is crushed with pulverizer, obtains porous SiC ceramics particulate.
A kind of 10. preparation method of water body purification of aquaculture agent, it is characterised in that：By 40~50 parts by weight photosynthetic bacteria agent,
10~20 parts by weight denitrifying bacteria agent, 10~20 parts by weight bacillus agent, 10~20 parts by weight moringa seeds extract solutions and 4~
6 parts by weight Chitosan/Cellulose complex microspheres produce after being stirred in sterile barrel.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201710908790.7A CN107673481B (en) | 2017-09-29 | 2017-09-29 | Aquaculture water body purifying agent and preparation method thereof |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201710908790.7A CN107673481B (en) | 2017-09-29 | 2017-09-29 | Aquaculture water body purifying agent and preparation method thereof |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN107673481A true CN107673481A (en) | 2018-02-09 |
| CN107673481B CN107673481B (en) | 2021-05-07 |
Family
ID=61138638
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201710908790.7A Active CN107673481B (en) | 2017-09-29 | 2017-09-29 | Aquaculture water body purifying agent and preparation method thereof |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN107673481B (en) |
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109956561A (en) * | 2019-04-04 | 2019-07-02 | 武汉轻工大学 | A kind of water quality cleansing agent and preparation method thereof |
| CN110468081A (en) * | 2019-09-17 | 2019-11-19 | 湖南艾布鲁环保科技股份有限公司 | Composite bacteria agent and its preparation method and application |
| CN110642380A (en) * | 2019-10-29 | 2020-01-03 | 福建师范大学 | Method for treating rare earth wastewater by microorganisms in large-scale outdoor pond |
| CN112920981A (en) * | 2021-04-23 | 2021-06-08 | 上海乾界生物科技有限公司 | Photosynthetic bacteria preparation |
Citations (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101269884A (en) * | 2008-05-08 | 2008-09-24 | 储卫华 | Microorganism renovation agent of water environment and preparation method thereof |
| CN101962229A (en) * | 2010-06-23 | 2011-02-02 | 天津生机集团股份有限公司 | Water purifying agent for pond culture |
| CN102212476A (en) * | 2011-03-07 | 2011-10-12 | 杭州向天歌生物科技有限公司 | Method for cleanly and efficiently producing microorganism bactericide |
| CN103695336A (en) * | 2013-12-05 | 2014-04-02 | 上海绿博生物科技发展有限公司 | Microbial preparation for improving bottom ecological environment of prawn pond and preparation method thereof |
| CN103922489A (en) * | 2014-05-09 | 2014-07-16 | 四川清和科技有限公司 | Microorganism composite flocculant coagulation bacterium application method |
| CN104530449A (en) * | 2014-12-18 | 2015-04-22 | 华南理工大学 | Cellulose/chitosan composite pellet as well as preparation method and application thereof |
| CN104649709A (en) * | 2013-11-17 | 2015-05-27 | 宋永法 | Method for manufacturing porous silicon carbide ceramic |
| CN105692843A (en) * | 2016-04-20 | 2016-06-22 | 上海海洋大学 | Water purifying agent on basis of proteins of moringa oleifera seeds and method for preparing water purifying agent |
-
2017
- 2017-09-29 CN CN201710908790.7A patent/CN107673481B/en active Active
Patent Citations (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101269884A (en) * | 2008-05-08 | 2008-09-24 | 储卫华 | Microorganism renovation agent of water environment and preparation method thereof |
| CN101962229A (en) * | 2010-06-23 | 2011-02-02 | 天津生机集团股份有限公司 | Water purifying agent for pond culture |
| CN102212476A (en) * | 2011-03-07 | 2011-10-12 | 杭州向天歌生物科技有限公司 | Method for cleanly and efficiently producing microorganism bactericide |
| CN104649709A (en) * | 2013-11-17 | 2015-05-27 | 宋永法 | Method for manufacturing porous silicon carbide ceramic |
| CN103695336A (en) * | 2013-12-05 | 2014-04-02 | 上海绿博生物科技发展有限公司 | Microbial preparation for improving bottom ecological environment of prawn pond and preparation method thereof |
| CN103922489A (en) * | 2014-05-09 | 2014-07-16 | 四川清和科技有限公司 | Microorganism composite flocculant coagulation bacterium application method |
| CN104530449A (en) * | 2014-12-18 | 2015-04-22 | 华南理工大学 | Cellulose/chitosan composite pellet as well as preparation method and application thereof |
| CN105692843A (en) * | 2016-04-20 | 2016-06-22 | 上海海洋大学 | Water purifying agent on basis of proteins of moringa oleifera seeds and method for preparing water purifying agent |
Non-Patent Citations (3)
| Title |
|---|
| 汪啸穆: "《陶瓷工艺学》", 31 August 2003, 中国轻工业出版社 * |
| 盛军: "《现代辣木生物学》", 31 January 2015, 云南科技出版社 * |
| 蒋挺大: "《甲壳素》", 31 May 1996, 中国环境科学出版社 * |
Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109956561A (en) * | 2019-04-04 | 2019-07-02 | 武汉轻工大学 | A kind of water quality cleansing agent and preparation method thereof |
| CN110468081A (en) * | 2019-09-17 | 2019-11-19 | 湖南艾布鲁环保科技股份有限公司 | Composite bacteria agent and its preparation method and application |
| CN110642380A (en) * | 2019-10-29 | 2020-01-03 | 福建师范大学 | Method for treating rare earth wastewater by microorganisms in large-scale outdoor pond |
| CN110642380B (en) * | 2019-10-29 | 2023-10-10 | 福州文泽生物科技有限公司 | Method for treating rare earth wastewater by using scale outdoor pool microorganisms |
| CN112920981A (en) * | 2021-04-23 | 2021-06-08 | 上海乾界生物科技有限公司 | Photosynthetic bacteria preparation |
Also Published As
| Publication number | Publication date |
|---|---|
| CN107673481B (en) | 2021-05-07 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| Luo et al. | Growth, digestive activity, welfare, and partial cost-effectiveness of genetically improved farmed tilapia (Oreochromis niloticus) cultured in a recirculating aquaculture system and an indoor biofloc system | |
| CN104803485B (en) | A kind of microbial compound enzyme water purification agent granule | |
| CN101157892B (en) | Production method of solid preparation for degradation of ammonia nitrogen and nitrite | |
| CN107673481A (en) | A kind of water body purification of aquaculture agent and preparation method thereof | |
| CN103992187A (en) | Biological bacterial fertilizer for preventing and treating moss in water body and preparation method thereof | |
| CN106380004B (en) | Aquaculture waters restoration of the ecosystem agent and preparation method thereof | |
| CN104386895A (en) | Water-quality improver for aquaculture and preparation method of water-quality improver | |
| CN106867933A (en) | The probiotics of purification of water quality and preparation and application in being cultivated to Environment of Litopenaeus vannamei Low | |
| CN101333499A (en) | Complex active bacterial biological water purifying a gent and method for preparing same | |
| CN109618997A (en) | The ecological purification method of oyster culture | |
| CN110002611A (en) | A kind of breeding water body regulator and preparation method thereof | |
| CN115895960A (en) | Strain for comprehensive planting and breeding of rice and fish and application thereof | |
| CN105886422A (en) | Bacillus subtilis BY7 and application thereof in degrading residual feeds and ammonia nitrogen | |
| CN101078004A (en) | Microorganism preparation for modifying water body by using bacterial and manufacturing method thereof | |
| CN105441348A (en) | New bacillus subtilis strain, microecological preparation and application | |
| CN107760612B (en) | Aspergillus niger yy07 strain and application thereof in solid fermentation production of acidic protease for feed | |
| CN102017905A (en) | Method for breeding pinaeus monodon fries | |
| CN108423838A (en) | One kind is for water-based microorganism formulation of ecological safety and preparation method thereof | |
| CN108913631A (en) | The Meng Shi pseudomonas strains CY06 and its probiotics of one plant of efficient nitrogen reduction and application | |
| Shan et al. | Ammonia and nitrite nitrogen removal in shrimp culture by Vibrio alginolyticus VZ5 immobilized in SA beads | |
| CN104293798B (en) | A kind of microorganism formulation and preparation method for being used to reduce that bottom mudcake is produced | |
| CN114317332B (en) | Aerobic photosynthetic bacteria and application thereof | |
| CN106244486B (en) | The bacillus of one high-efficiency degradation nitrogen pollutant and its composite bacteria preparation | |
| CN109179666A (en) | A kind of preparation method of freshwater crayfish cultivation nutrition water adjusting agent | |
| CN108484239A (en) | A kind of method of resistance gene contamination in quick reduction fowl and animal excrement |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| CB03 | Change of inventor or designer information |
Inventor after: Liu Aimin Inventor after: Zhou Aimin Inventor after: He Youyi Inventor after: Qiao Yindi Inventor before: Qiao Yindi |
|
| CB03 | Change of inventor or designer information | ||
| TA01 | Transfer of patent application right |
Effective date of registration: 20210408 Address after: 225300 No.1, Hongqi Avenue, Taizhou City, Jiangsu Province Applicant after: Jiangsu haorun Biological Industry Group Co.,Ltd. Address before: 201202 No. 30, feijiazhai, Zhongwei village, airport town, Pudong New Area, Shanghai Applicant before: Qiao Yindi |
|
| TA01 | Transfer of patent application right | ||
| GR01 | Patent grant | ||
| GR01 | Patent grant |