CN105692843A - Water purifying agent on basis of proteins of moringa oleifera seeds and method for preparing water purifying agent - Google Patents

Abstract

The invention relates to a water body purifying agent based on Moringa oleifera seed protein and a preparation method thereof. The water body purifying agent is made of the following raw materials in parts by weight: 1 part of Moringa oleifera seed protein and 1 part of activated carbon. The invention extracts and processes the active protein components of Moringa oleifera seeds, mixes them with a certain proportion of activated carbon powder, and applies them to waste water treatment and water purification with different turbidities. landscape effect. The water purifying agent of the present invention is directly and evenly spread in the water body when used, and the specific application amount depends on the turbidity of the water body. The final water purification effect can reach 95%, and the turbidity is less than 3NTU, which meets the national drinking water standard.

Description

A water purifying agent based on Moringa seed protein and its preparation method

technical field

The invention relates to the technical field of environmental protection water purification, in particular to a water purifying agent based on Moringa oleifera seed protein and a preparation method thereof.

Background technique

With the rapid development of social economy and the continuous growth of population, the shortage of water resources and the pollution of water environment are becoming more and more serious. In order to meet the needs of industrial and agricultural production and people's daily life, and improve the health level, water conservation, water pollution treatment and reuse are particularly important. Flocculation technology has been widely used in drinking water treatment, production wastewater treatment, food and cosmetics and other fields. It achieves the purpose of water purification by forming larger flocs from charged colloidal particles in water. Water purification through flocculation technology has quick results and is easy to use. Convenience, low cost and other advantages. Currently commonly used flocculants mainly include inorganic flocculants (polyaluminum, polyferric, etc.), inorganic polymer flocculants (polyaluminum chloride, polyferric sulfate, etc.) and organic polymers (polyacrylamide series, polyquaternium Salt, etc.), these flocculants have a good coagulation and decolorization effect, but when the above water purification flocculants are used, the residual metal ions and PAM monomers will cause secondary pollution, which will have a certain impact on the ecological environment and damage human health.

Chinese patent 201110130572.8 discloses a Moringa water purifier, which is composed of the following components in mass ratio: Moringa extract 90-100%, chemical water purifier 0-10%, which makes full use of the remaining The active ingredients of Moringa oleifera are extracted, and the complete comprehensive utilization of Moringa oleifera seeds is realized. This patent adopts the frying method to extract the components of Moringa oleifera, and the components of the extract are complex, which increases other impurities in the water. At the same time, chemical water purifier components are added to the water purifier, which has a serious problem of secondary pollution. The paper "Research on the Purification Effect of Natural Plant Moringa Seeds on Water Body" published in the Chinese journal "Journal of Hefei University of Technology" in February 2012, its natural water purifiers of Moringa seeds and common chemical water purifiers alum, polyaluminum chloride The results show that the Moringa seed has a more obvious water purification effect, and the water purification efficiency is equivalent to that of polyaluminium chloride; the Moringa seed extract has a good water purification effect, but the organic matter content in the water body increases. . The inventor found during the experiment that although Moringa seeds have water purification effect, after using Moringa seeds for water purification for more than one month, the content of COD _Mn and nitrate nitrogen in the water body increases, thereby promoting the growth of organic matter and affecting water quality. Therefore for the shortcoming of prior art, it is necessary to develop a kind of water purifying agent based on Moringa seed protein, not only can improve the water purifying effect of Moringa seed, can not produce secondary pollution to water quality simultaneously, and about this class purifying agent at present Not yet reported.

Contents of the invention

The object of the present invention is to provide a water body ecological purifier, which has the advantages of non-toxicity, degradability and environmental friendliness. Not only does it have no effect on the pH value and conductivity of the purified water, but it is also stable at higher temperatures. During the water purification process, it can also remove microorganisms and heavy metal ions such as Cd, Ni, and Cr in the water. The volume of flocculation waste generated after water purification of Moringa oleifera seeds is only 1/3 of that of chemical water purifiers, and the flocculation waste can be used as fertilizer without causing environmental pollution.

The purpose of the present invention is to provide a kind of water purifying agent based on moringa seed protein aiming at the deficiencies in the prior art.

For realizing above-mentioned object, the technical scheme that the present invention takes is:

A water body purifying agent based on moringa seed protein, said water body purifying agent comprising moringa seed protein.

The water body purifying agent also includes the following components: polyaluminum chloride, polyferric sulfate, polyacrylamide or activated carbon.

Preferably, the purifying agent also includes the following components: activated carbon.

Preferably, the water body purifying agent is made of the following raw materials in parts by weight: 1 part of Moringa seed protein and 1 part of activated carbon.

The preparation method of described water body purifying agent is as follows:

1) extract Moringa seed protein from Moringa seed;

2) Utilize the cooling crystallization method to make the Moringa oleifera seed protein extracted in step 1) crystallize into powder;

3) step 2) Moringa seed protein powder and activated carbon powder after crystallization are passed through 400 mesh molecular sieves respectively;

4) Evenly mix the sieved Moringa seed protein powder and activated carbon powder in a mass ratio of 1:1;

5) Divide the powder mixture prepared in step 4) to obtain the water body purifying agent.

Wherein, the Moringa oleifera seed protein extraction method of described step 1) is as follows:

Shell the Moringa seeds, pulverize them with a grinder, dry the Moringa seed powder, extract the Moringa seed components with 95% ethanol, stir thoroughly for 30 minutes, take the precipitate and dry it at room temperature, and use 0.5mol/L NaCl solution with the dried Moringa seeds The precipitate was stirred for 30 minutes, filtered through Whatman filter paper No. 1, and then filtered with Whatman 0.45 μm glass fiber filter paper, and the filtered liquid was Moringa seed protein.

The activated carbon is activated.

After the water body purification agent of the present invention is put into the water body to be purified, the potassium permanganate index and the nitrate nitrogen content of the water body do not increase compared with those before the water body is put into use.

A water body purifying agent, comprising any one of the above water body purifying agents, and conventional excipients, carriers or diluents.

In the early stage of the experiment, the inventor mixed Moringa seed protein with a variety of compounds with water purification activity, and found that after mixing Moringa seed protein with activated carbon, it could not only improve the water purification effect, but also solve the problem of water quality after using Moringa seed for water purification. The problem of increased organic content. Compared with using Moringa seed extract alone to purify water, Moringa seed extract + activated carbon water purifier has a significant effect on reducing water turbidity, while potassium permanganate index and nitrate nitrogen content did not increase. Compared with Moringa seed extract, Moringa seed extract mixed with activated carbon is more suitable as a water purifier.

The present invention has the advantage that:

1. The present invention extracts and processes the active protein components of Moringa oleifera seeds, mixes them with a certain proportion of activated carbon powder, and applies them to wastewater treatment and water purification with different turbidities. The effect is obvious, and there is no secondary pollution to water quality, and it can also Improve the effect of water landscape.

2. The present invention mixes Moringa seed protein with activated carbon powder, which effectively solves the problem of organic matter in water produced by a single Moringa seed, improves water purification effect, and does not cause secondary pollution to water quality.

3. The water purifying agent of the present invention is directly and evenly spread in the water body when used. The specific application amount depends on the degree of turbidity of the water body. The final water purification effect can reach 95%, and the turbidity is less than 3NTU, which meets the national drinking water standard.

Description of drawings

Accompanying drawing 1 is the water purification effect diagram of Moringa oleifera seed, and the test tube on the left side in the figure is the turbid water added with the crude extract of Moringa oleifera seed protein, and the right side in the figure is turbid water under the natural state.

detailed description

The present invention will be further described below in combination with specific embodiments. It should be understood that these examples are only used to illustrate the present invention and are not intended to limit the scope of the present invention. In addition, it should be understood that after reading the contents of the present invention, those skilled in the art can make various changes or modifications to the present invention, and these equivalent forms also fall within the scope defined by the appended claims of the present application.

Embodiment 1 Moringa seed is to water purification effect

1 Materials and methods

1.1 Experimental materials and instruments

Moringa seeds are the seeds of Moringa oleifera, and the activated carbon powder is purchased from Lanrun Environmental Protection Technology Co., Ltd. The model of the turbidimeter is HANNAHI91703.

1.2 Experimental method

1.2.1 Water sample preparation

Take 100g (wet weight) of soil samples (wet weight) at the normal water level of the slope of the artificial landscape lake of Shanghai Ocean University (water surface 60000m ² , average water depth 1.6m), put it in an oven for drying at 105°C, cool to temperature, and use a pulverizer to dry it. Grind to a particle size of 0.1mm, weigh 5g of the soil sample, place it in an evaporating dish, soak it with distilled water, add 1000mL of lake water after 2 days, stir evenly with an electronic shaker, and after standing for 2 hours, transfer all the upper layer of muddy water into a 1000mL volumetric flask Keep the volume in the medium for standby, so as to obtain a muddy water sample with a certain turbidity.

1.2.2 Moringa water purification experiment

Moringa seeds compared to chemical water purifiers. Commonly used water purifying agents such as polyaluminum chloride and alum were selected to compare the water purifying effect with Moringa water purifying agent to determine their water purifying function. After the Moringa seeds are shelled, crush them with a grinder, weigh 10.00mg of Moringa seed powder, PAM, alum, and polymerized AlCl ₃ respectively, put them in a 200mL beaker, add 100mL of muddy water samples, and stir for 5min with a shaker at a speed of 500r /min, use a turbidimeter to measure the initial turbidity of the muddy water sample, measure once every 30 minutes after standing still, repeat 3 times for each sample, and take the average value. At the same time, the mixed water sample without water purifying agent was used as the control.

1.2.3 The water purification effect of the ingredients extracted from Moringa oleifera seeds. Moringa seeds are shelled, pulverized by a pulverizer, dried Moringa seed powder is extracted with 95% ethanol, and fully stirred for 30 minutes, and the precipitate is dried at room temperature, and then mixed with 0.5mol/L NaCl solution and dried Moringa The seed precipitate was stirred for 30 minutes, filtered through Whatman filter paper No. 1, and then filtered with Whatman 0.45 μm glass fiber filter paper, and the filtered liquid was the Moringa oleifera seed extract. Add 100mL of distilled water and Moringa oleifera seed extract to 2 beakers with 100mL of muddy water samples respectively, stir with a shaker for 5min at a speed of 500r/min, and measure the initial turbidity of the muddy water samples. Measure once every 60 minutes, repeat every other sample 3 times, take the average value, and finally measure the COM _Mn of the water body.

1.2.4 Moringa seed extract components and activated carbon water purification effect

The ingredients of the Moringa seed extract are the same as in section 1.2.3; take the Moringa seed extract and use the cooling crystallization method to crystallize the extract into a powder; pass the crystallized Moringa seed protein powder and activated carbon powder through a 400-mesh molecular sieve; The processed Moringa seed protein powder and activated carbon powder are evenly mixed according to the mass ratio of 1:1 to obtain the mixed water purifier. Weigh 10.00mg of mixed water purifier, put it in a 200mL beaker, add 100mL of muddy water sample, stir with a shaker for 5min at a speed of 500r/min, use a turbidimeter to measure the initial turbidity of the muddy water sample, after standing Measured once every 30min, each sample was repeated 3 times, and the average value was taken.

1.3 Detection method

1.3.1 COD determination

The permanganate index is often used as a comprehensive indicator of the pollution degree of surface water bodies by organic pollutants and reducing inorganic substances. The COM _Mn in water bodies is determined according to the national standard GB11892-89 permanganate index method.

1.3.2 Turbidity determination

Turbidity refers to the degree of obstruction of suspended matter in water when light passes through. The turbidity caused by human life and industrial domestic sewage increases, and the surface water quality deteriorates. Use a turbidity meter for detection.

1.3.3 Determination of nitrate nitrogen

Nitrate nitrogen refers to the nitrogen element contained in nitrate. The content of nitrate nitrogen in water was determined by spectrophotometry.

2 results

2.1 Analysis of nutritional components of Moringa seeds

The nutritional components of Moringa oleifera seeds introduced and planted in 3 counties of Yunnan Province were determined, and the content ratios are shown in Table 1.

Table 1 Analysis table of nutritional components of Moringa seeds

Element Content ratio total sugar 6.9%～9.5% protein 36.2%～38.7% Fat 33.8%～38.2% Potassium 515.3～617.6mg/100g calcium 95.0～135.3mg/100g iron 2.9～3.5mg/100g

It can be seen from Table 1 that Moringa seeds are rich in protein, and contain trace elements such as potassium, calcium, and iron that are beneficial to the human body. Rich protein content provides favorable conditions for protein extraction.

2.1 Comparison of the effect of Moringa seed extract and chemical water purifier on reducing water turbidity

The initial value of the turbidity of the control turbid water sample was 194NTU, and the crude extract of Moringa oleifera seed, polyaluminum chloride, and alum were added respectively, and another group of control experiments was set up. After stirring and standing for 30 minutes, the accuracy of the mixed water sample was measured. The test results are shown in Figure 1 and Table 2.

Table 2 Comparison of the effects of Moringa oleifera seed extracts and chemical water purifiers on reducing water turbidity

In Figure 1, the test tube on the left is the turbid water added with the crude extract of Moringa oleifera seed protein, and the right side of the figure is the turbid water in the natural state. As can be seen from the results in Table 2, Moringa seeds have a very obvious water purification effect.

2.2 Toxicity test of the crude extract of Moringa oleifera seeds

Toxicity test of the crude extract of Moringa oleifera seeds: The components of the crude extract of Moringa oleifera seeds are complex, among which the contents of COD _Mn and nitrate nitrogen are relatively high. Excessive COD content in water will affect the biological stability of water quality and cause water quality corruption , a large amount of nitrate nitrogen will cause eutrophication of the water body, so the experiment measures the content changes of COD _Mn and nitrate nitrogen before and after the water purification of Moringa oleifera seeds (unit: mg/L), and the results are as follows shown in the table.

Table 3 The effect of Moringa oleifera seed extract components on water permanganate after use

Table 4 The effect of Moringa oleifera seed extract components on water body nitrate nitrogen after use

As can be seen from the results in Table 3 and Table 4, compared with the muddy water sample without water purifying agent, the crude extract of Moringa oleifera seeds increased the COD _Mn and nitrate nitrogen content in the muddy water sample; While reducing the turbidity of the water body, the liquid also increases the content of organic matter in the water body, which affects the water quality. Compared with the extract of Moringa oleifera seed, the extract of Moringa oleifera seed + activated carbon water purifier has a significant effect on reducing water turbidity, while the potassium permanganate index and nitrate nitrogen content did not increase. Compared with Moringa seed extract, Moringa seed extract mixed with activated carbon is more suitable as a water purifier.

The above is only a preferred embodiment of the present invention, it should be pointed out that for those of ordinary skill in the art, without departing from the method of the present invention, some improvements and supplements can also be made, and these improvements and supplements should also be considered Be the protection scope of the present invention.

Claims (9)

1. A water body purifying agent based on Moringa seed protein, characterized in that: the water body purifying agent comprises Moringa seed protein. 2. The water body purifying agent based on Moringa seed protein according to claim 1, characterized in that: the water body purifying agent further comprises the following components: polyaluminum chloride, polyferric sulfate, polyacrylamide or activated carbon. 3. The water body purifying agent based on Moringa seed protein according to claim 1, characterized in that: the water body purifying agent also comprises activated carbon. 4. The water body purifying agent based on Moringa seed protein according to claim 3, characterized in that: the water body purifying agent is made of the following raw materials in parts by weight: 1 part of Moringa seed protein and 1 part of activated carbon. 5. the water body purifier based on Moringa seed protein according to claim 4, is characterized in that: the preparation method of described water body purifier is as follows: 1) extract Moringa seed protein from Moringa seed; 2) Utilize the cooling crystallization method to make the Moringa oleifera seed protein extracted in step 1) crystallize into powder; 3) step 2) Moringa seed protein powder and activated carbon powder after crystallization are passed through 400 mesh molecular sieves respectively; 4) Evenly mix the sieved Moringa seed protein powder and activated carbon powder in a mass ratio of 1:1; 5) Divide the powder mixture prepared in step 4) to obtain the water body purifying agent. 6. according to the described water purifying agent of Moringa seed protein based on claim 5, it is characterized in that: the extraction method of Moringa seed protein of described step 1) is as follows: Shell the Moringa seeds, pulverize them with a grinder, dry the Moringa seed powder, extract the Moringa seed components with 95% ethanol, stir thoroughly for 30 minutes, take the precipitate and dry it at room temperature, and use 0.5mol/L NaCl solution with the dried Moringa seeds The precipitate was stirred for 30 minutes, filtered through Whatman filter paper No. 1, and then filtered with Whatman 0.45 μm glass fiber filter paper, and the filtered liquid was Moringa seed protein. 7. according to the described water body purification agent based on Moringa seed protein of claim 6, it is characterized in that: after the water body that needs to be purified is put into water body purification agent, the potassium permanganate index of water body and nitrate nitrogen content are compared with before putting in No elevation. 8. The water body purifying agent based on Moringa seed protein according to claim 4, characterized in that: the activated carbon has been activated. 9. A water body purifying agent, characterized in that it comprises the water body purifying agent according to any one of claims 1-8, and conventional excipients, carriers or diluents.