CN105692843A - Water purifying agent on basis of proteins of moringa oleifera seeds and method for preparing water purifying agent - Google Patents
Water purifying agent on basis of proteins of moringa oleifera seeds and method for preparing water purifying agent Download PDFInfo
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- CN105692843A CN105692843A CN201610246274.8A CN201610246274A CN105692843A CN 105692843 A CN105692843 A CN 105692843A CN 201610246274 A CN201610246274 A CN 201610246274A CN 105692843 A CN105692843 A CN 105692843A
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- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F1/00—Treatment of water, waste water, or sewage
- C02F1/52—Treatment of water, waste water, or sewage by flocculation or precipitation of suspended impurities
- C02F1/5236—Treatment of water, waste water, or sewage by flocculation or precipitation of suspended impurities using inorganic agents
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- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F1/00—Treatment of water, waste water, or sewage
- C02F1/52—Treatment of water, waste water, or sewage by flocculation or precipitation of suspended impurities
- C02F1/5263—Treatment of water, waste water, or sewage by flocculation or precipitation of suspended impurities using natural chemical compounds
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- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F1/00—Treatment of water, waste water, or sewage
- C02F1/52—Treatment of water, waste water, or sewage by flocculation or precipitation of suspended impurities
- C02F1/54—Treatment of water, waste water, or sewage by flocculation or precipitation of suspended impurities using organic material
- C02F1/56—Macromolecular compounds
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- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
- C07K1/14—Extraction; Separation; Purification
- C07K1/145—Extraction; Separation; Purification by extraction or solubilisation
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- Environmental & Geological Engineering (AREA)
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- Hydrology & Water Resources (AREA)
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- Proteomics, Peptides & Aminoacids (AREA)
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Abstract
The invention relates to a water purifying agent on the basis of proteins of moringa oleifera seeds and a method for preparing the water purifying agent. The water purifying agent comprises, by weight, 1 part of the proteins of the moringa oleifera seeds and 1 part of activated carbon. The water purifying agent and the method have the advantages that active protein components of the moringa oleifera seeds are extracted and treated and are mixed with activated carbon powder according to certain proportions to obtain the water purifying agent, the water purifying agent can be applied to treating wastewater and purifying water with different turbidity degrees, accordingly, obvious effects can be realized, the water purifying agent is free of secondary pollution on the water quality, and water landscape effects further can be improved; the water purifying agent is directly scattered in the water when used, the particular application amount of the water purifying agent is determined according to the turbidity degrees of the water, ultimate water purification effects can reach 95%, the turbidity of the treated water is lower than 3 NTU, and requirements of national drinking water standards can be met.
Description
Technical field
The present invention relates to environmental protection technical field of water purification, specifically, be a kind of Water body cleansing agent based on moringa seeds albumen and preparation method thereof。
Background technology
Constantly increasing with population along with social economy is fast-developing, shortage of water resources and water environment pollution increase the weight of day by day, for meeting industrial and agricultural production and people's daily life, improve health conditions, and using water wisely, water pollution process and reuse are particularly important。Flocculation technique is widely used to drinking water treatment, production wastewater treatment, the fields such as foods and cosmetics, it reaches the purpose of water purification by charged colloidal particles in water is formed bigger floccule body, has instant effect, the advantage such as easy to use, less costly by flocculation technique water purification。Flocculant conventional at present mainly has inorganic flocculating agent (polymeric aluminum, polymeric iron etc.), inorganic polymer flocculant (aluminium polychlorid, bodied ferric sulfate etc.) and organic high molecular polymer (polyacrylamide series, polyquaternary ammonium salt etc.), these flocculant have good cohesion decolorizing effect, but above-mentioned water purifying flocculating agent is while using, the metal ion of its residual and PAM monomer etc. can cause secondary pollution, ecological environment is produced certain impact, damages health。
Chinese patent 201110130572.8 discloses a kind of Moringa oleifera water purifying agent, it is made up of the component of following masses ratio: Moringa extract 90～100%, chemistry water purification agent 0～10%, it makes full use of the residue after Moringa oil expression, make Moringa extracts active ingredients therein out, it is achieved that the total integration of Moringa seed utilizes。This patent adopts frying method to extract Moringa composition, and extract component is complicated, makes to add other impurity in water, with the addition of chemistry water purification agent composition simultaneously, there are the serious problems of secondary pollution in water purification agent。The paper " research to water body purification effect of the natural plants moringa seeds " of publication Chinese periodical " HeFei University of Technology's journal " in February, 2012, the natural water purification agent of moringa seeds and purifying water effects such as commonly using chemistry water purifier Alumen, aluminium polychlorid are compared research by it, result shows that moringa seeds has obvious purifying water effect, and cleaning water is suitable with aluminium polychlorid；Moringa seeds extracting solution has good purifying water effect, but in water body, content of organics increases to some extent。Inventor finds in experimentation: though moringa seeds has purifying water effect, but after using more than one moon moringa seeds water purification time, COD in water bodyMn, nitrate nitrogen equal size increase, thus having promoted organic growing, affect water quality。Shortcoming hence for prior art, it is necessary to research and develop a kind of Water body cleansing agent based on moringa seeds albumen, can not only improve moringa seeds purifying water effect, water quality will not produce secondary pollution simultaneously, and yet there are no report about such cleanser。
Summary of the invention
The invention aims to provide a kind of water ecology cleanser, there is nontoxic, degradable, environment amenable advantage。It not only pH value and electric conductivity on institute's scrubbing water do not affect, also very stable at relatively high temperatures, Water treatment can also remove the heavy metal ion such as microorganism in water, and Cd, Ni, Cr。After moringa seeds water purification, produced flocculation Waste volume is only the 1/3 of chemistry water purification agent, and this flocculation refuse can be used as fertilizer with this flocculation refuse, does not cause environmental pollution。
It is an object of the invention to for deficiency of the prior art, it is provided that a kind of Water body cleansing agent based on moringa seeds albumen。
For achieving the above object, the present invention adopts the technical scheme that:
A kind of Water body cleansing agent based on moringa seeds albumen, described Water body cleansing agent comprises moringa seeds albumen。
Described Water body cleansing agent also comprises following composition: aluminium polychlorid, bodied ferric sulfate, polyacrylamide or activated carbon。
Preferably, described cleanser also comprises following composition: activated carbon。
Preferably, described Water body cleansing agent is made up of the raw material of following weight portion: 1 part of moringa seeds albumen, activated carbon 1 part。
The preparation method of described Water body cleansing agent is as follows:
1) from moringa seeds, moringa seeds albumen is extracted；
2) lowering temperature crystallization is utilized to make step 1) the moringa seeds protein crystal that extracts is powdered；
3) by step 2) moringa seeds protein powder after crystallization and active carbon powder cross 400 order molecular sieves respectively；
4) by the moringa seeds protein powder after sieving and active carbon powder 1:1 Homogeneous phase mixing in mass ratio；
5) by step 4) namely the powder mixture subpackage prepared obtain Water body cleansing agent。
Wherein, described step 1) moringa seeds protein extracting method as follows:
Moringa seeds shells, pulverizer is pulverized, dry moringa seeds powder 95% ethanol extraction moringa seeds composition, it is sufficiently stirred for 30min, taking precipitate is in drying at room temperature, with the NaCl solution of 0.5mol/L and dry moringa seeds precipitate stirring 30min, by Whatman filter paper 1 filtration, filtering by Whatman0.45 μm of glass fiber filter paper, the liquid after filtration is moringa seeds albumen again。
Described activated carbon processes through activity。
After throwing in Water body cleansing agent of the present invention in needing the water body purified, the permanganate indices of water body does not raise compared with before input with nitrate nitrogen content。
A kind of Water body cleansing agent, bag is arbitrary described Water body cleansing agent and conventional excipients, carrier or diluent as above。
Moringa seeds albumen is mixed by inventor's phase on pretreatment respectively with the multiple compound with water purification activity, after moringa seeds albumen is mixed by discovery with activated carbon, purifying water effect can not only be improved, solve the problem that after using moringa seeds water purification, water body organism content increases simultaneously。Compared with being used alone moringa seeds extract component water purification, it is obvious that moringa seeds extract component+activated carbon water purification agent reduces water turbidity effect, and permanganate indices and nitrate nitrogen content do not increase simultaneously。Compared to moringa seeds extract component, moringa seeds extract component mixed active charcoal is preferably as water purification agent。
The invention has the advantages that:
1, the present invention passes through the extraction to moringa seeds activated protein composition and process, and mixes with certain proportion active carbon powder, applies in wastewater treatment and different turbidity water body purification, and effect is obvious, and to water quality non-secondary pollution, also can improve water landscape effect。
2, moringa seeds albumen is mixed by the present invention with active carbon powder, effectively solves single moringa seeds and produces organic problem in water body, improve purifying water effect, water quality will not be produced secondary pollution simultaneously。
3, when the water purification agent of the present invention uses, Direct Uniform is spread out in water body, and concrete amount of application is depending on water body muddiness degree, and final purifying water effect can reach 95%, and < 3NTU meets national drinking water standard to turbidity。
Accompanying drawing explanation
Accompanying drawing 1 is that in moringa seeds purifying water effect figure, figure, left side test tube is the muddy water adding moringa seeds albumen crude extract, and in figure, right side is muddy water under naturalness。
Detailed description of the invention
Below in conjunction with detailed description of the invention, the present invention is expanded on further。Should be understood that these embodiments are merely to illustrate the present invention rather than restriction the scope of the present invention。In addition, it is to be understood that after having read the content that the present invention records, the present invention can be made various changes or modifications by those skilled in the art, and these equivalent form of values fall within the application appended claims limited range equally。
Embodiment 1 moringa seeds is to water body purification effect
1 material and method
1.1 experiment materials and instrument
Moringa seeds is the seed of Moringa plant Moringa, and active carbon powder is purchased from Lan Run Environmental Protection Technology Co., Ltd。Transmissometer model is HANNAHI91703。
1.2 experimental techniques
1.2.1 water sample preparation
Take Shanghai Ocean University's artificial landscape lake (water surface 60000m2Mean depth 1.6m) the soil sample 100g (weight in wet base) at side slope ordinary water level place, put under 105 DEG C of conditions in baking oven and dry, be cooled to temperature, grind to form 0.1mm particle diameter with pulverizer, weigh 5g soil sample to be placed in evaporating dish and add distilled water infiltration, add lake water 1000mL after 2d, stir with Electric cradle, after standing 2h, upper strata muddy water liquid is all proceeded to constant volume in 1000mL volumetric flask standby, thus obtaining the muddy water sample of certain turbidity。
1.2.2 Moringa water purification experiment
Moringa seeds compares with chemistry water purification agent。Select the conventional water purification agent such as aluminium polychlorid, Alumen to carry out purifying water effect with Moringa oleifera water purifying agent to compare, it is determined that its water purification function。Moringa seeds is pulverized with pulverizer after shelling, and weighs 10.00mg moringa seeds powder, PAM, Alumen, polymerization AlCl respectively3, it being placed in 200mL beaker, add the muddy water sample of 100mL, stir 5min, rotating speed 500r/min with shaking table, measure the initial turbidity of muddy water sample with transmissometer, measure 1 time every 30min after standing, each sample repeats for 3 times, averages。Simultaneously with do not add water purification agent mixed water sample for comparison。
1.2.3 moringa seeds extract component purifying water effect。Moringa seed shells, pulverizer is pulverized, dry Moringa seed powder 95% ethanol extraction moringa seeds composition, it is sufficiently stirred for 30min, taking precipitate is in drying at room temperature, afterwards with the NaCl solution of 0.5mol/L and dry moringa seeds precipitation stirring 30min, by Whatman filter paper 1 filtration, filtering by Whatman0.45 μm of glass fiber filter paper, the liquid after filtration is moringa seeds extract again。In 2 beakers being placed with 100mL muddy water sample respectively, it is separately added into 100mL distilled water and moringa seeds extracting solution, stirs 5min with shaking table, rotating speed 500r/min, measures the initial turbidity of muddy water sample, measures 1 time every 60min after standing, repeat for 3 times every sample, average, finally measure water body COMMn。
1.2.4 moringa seeds extract component and activated carbon purifying water effect
Moringa seeds extract component saves with 1.2.3；Taking moringa seeds extracting solution uses lowering temperature crystallization to be crystallized into by extracting solution Powdered；Moringa seeds protein powder after crystallization and active carbon powder are crossed 400 order molecular sieves respectively；The moringa seeds protein powder being sieving through is mixed homogeneously according to mass ratio 1:1 ratio with active carbon powder, obtains mixing water purification agent。Weighing 10.00mg mixing water purification agent, be placed in 200mL beaker, add the muddy water sample of 100mL, stir 5min, rotating speed 500r/min with shaking table, measure the initial turbidity of muddy water sample with transmissometer, measure 1 time every 30min after standing, each sample repeats for 3 times, averages。
1.3 detection methods
1.3.1COD measure
Permanganate index is often taken as the surface water body aggregative indicator by organic pollution and reducing inorganic Substances Pollution degree, measures COM in water body according to GB GB11892-89 Permanganate Index MethodMn。
1.3.2 turbidimetric analysis turbidimetry
Turbidity refers to the obstruction degree occurred when float is to light therethrough in water。The turbidity that human lives and industry sanitary sewage cause increases, and surface water quality becomes bosom, utilizes transmissometer to detect。
1.3.3 nitrate nitrogen measures
Nitrate nitrogen refers to nitrogen element contained in nitrate。Nitrate nitrogen in water body content spectrophotometry。
2 results
2.1 moringa seeds Analysis of Nutritive Compositions
County of 3, Yunnan Province being introduced a fine variety the moringa seeds nutritional labeling planted be measured, its content ratio is as shown in table 1。
Table 1 moringa seeds Analysis of Nutritive Composition table
| Composition | Content ratio |
| Total sugar | 6.9%～9.5% |
| Protein | 36.2%～38.7% |
| Fat | 33.8%～38.2% |
| Potassium | 515.3～617.6mg/100g |
| Calcium | 95.0～135.3mg/100g |
| Ferrum | 2.9～3.5mg/100g |
As known from Table 1, containing rich in protein in moringa seeds, and containing the trace elements K that human body is useful, calcium, ferrum etc.。Rich in protein content provides advantage to protein extraction。
2.1 moringa seeds extract component reduce water turbidity effectiveness comparison with chemistry water purification agent
Comparison turbid water sample turbidity initial value is 194NTU, is separately added into moringa seeds crude extract, aluminium polychlorid, Alumen, separately sets one group of controlled trial, by stirring, starts to measure mixed water sample accuracy after standing 30min, and its testing result is shown in Fig. 1 and table 2。
Table 2 moringa seeds extract component reduces water turbidity effectiveness comparison with chemistry water purification agent
In Fig. 1, left side test tube is the muddy water adding moringa seeds albumen crude extract, and in figure, right side is muddy water under naturalness。As can be known from the results of Table 2, moringa seeds has obviously purifying water effect。
The toxicity test of 2.2 moringa seeds crude extracts
The toxicity test research of moringa seeds crude extract: moringa seeds crude extract complicated component, wherein CODMnHigh with nitrate nitrogen two indices comparision contents, in water, COD too high levels can affect the biological stability of water quality, causes water quality corrupt, and a large amount of nitrate nitrogens can cause body eutrophication, and therefore measuring threw in water purification agent after 1 month, CODMnWith nitrate nitrogen changes of contents (unit: mg/L) before and after moringa seeds water purification, result is as shown in the table。
Table 3 moringa seeds extract component use after impact on water body permanganate
Table 4 moringa seeds extract component use after impact on water body nitrate nitrogen
From the result of table 3 and table 4 it can be seen that compared with the muddy water sample not adding water purification agent, moringa seeds crude extract adds COD in muddy water sampleMnAnd nitrate nitrogen content；Moringa seeds crude extract, while reducing water turbidity, too increases water body organism content, water quality is affected to some extent。Compared with moringa seeds extract component, it is obvious that moringa seeds extract component+activated carbon water purification agent reduces water turbidity effect, and permanganate indices and nitrate nitrogen content do not increase simultaneously。Compared to moringa seeds extract component, moringa seeds extract component mixed active charcoal is preferably as water purification agent。
The above is only the preferred embodiment of the present invention; it should be pointed out that, for those skilled in the art, under the premise without departing from the inventive method; can also making some improvement and supplement, these improve and supplement and also should be regarded as protection scope of the present invention。
Claims (9)
1. the Water body cleansing agent based on moringa seeds albumen, it is characterised in that: described Water body cleansing agent comprises moringa seeds albumen。
2. according to claim 1 based on the Water body cleansing agent of moringa seeds albumen, it is characterised in that: described Water body cleansing agent also comprises following composition: aluminium polychlorid, bodied ferric sulfate, polyacrylamide or activated carbon。
3. according to claim 1 based on the Water body cleansing agent of moringa seeds albumen, it is characterised in that: described Water body cleansing agent also comprises activated carbon。
4. according to claim 3 based on the Water body cleansing agent of moringa seeds albumen, it is characterised in that: described Water body cleansing agent is made up of the raw material of following weight portion: 1 part of moringa seeds albumen, activated carbon 1 part。
5. according to claim 4 based on the Water body cleansing agent of moringa seeds albumen, it is characterised in that: the preparation method of described Water body cleansing agent is as follows:
1) from moringa seeds, moringa seeds albumen is extracted；
2) lowering temperature crystallization is utilized to make step 1) the moringa seeds protein crystal that extracts is powdered；
3) by step 2) moringa seeds protein powder after crystallization and active carbon powder cross 400 order molecular sieves respectively；
4) by the moringa seeds protein powder after sieving and active carbon powder 1:1 Homogeneous phase mixing in mass ratio；
5) by step 4) namely the powder mixture subpackage prepared obtain Water body cleansing agent。
6. according to claim 5 based on the Water body cleansing agent of moringa seeds albumen, it is characterised in that: described step 1) moringa seeds protein extracting method as follows:
Moringa seeds shells, pulverizer is pulverized, dry moringa seeds powder 95% ethanol extraction moringa seeds composition, it is sufficiently stirred for 30min, taking precipitate is in drying at room temperature, with the NaCl solution of 0.5mol/L and dry moringa seeds precipitate stirring 30min, by Whatman filter paper 1 filtration, filtering by Whatman0.45 μm of glass fiber filter paper, the liquid after filtration is moringa seeds albumen again。
7. according to claim 6 based on the Water body cleansing agent of moringa seeds albumen, it is characterised in that: after the water body needing to purify throws in Water body cleansing agent, the permanganate indices of water body does not raise compared with before input with nitrate nitrogen content。
8. according to claim 4 based on the Water body cleansing agent of moringa seeds albumen, it is characterised in that: described activated carbon processes through activity。
9. a Water body cleansing agent, it is characterised in that: comprise the arbitrary described Water body cleansing agent of claim 1-8 and conventional excipients, carrier or diluent。
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| CN106348360A (en) * | 2016-10-26 | 2017-01-25 | 商丘师范学院 | Wastewater cleaning agent based on photocatalysis |
| CN107673481A (en) * | 2017-09-29 | 2018-02-09 | 乔银娣 | A kind of water body purification of aquaculture agent and preparation method thereof |
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| CN107673481A (en) * | 2017-09-29 | 2018-02-09 | 乔银娣 | A kind of water body purification of aquaculture agent and preparation method thereof |
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Application publication date: 20160622 |