CN101658252A - Food organism additive for cultivating stichopus japonicus - Google Patents
Food organism additive for cultivating stichopus japonicus Download PDFInfo
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- CN101658252A CN101658252A CN200910187417A CN200910187417A CN101658252A CN 101658252 A CN101658252 A CN 101658252A CN 200910187417 A CN200910187417 A CN 200910187417A CN 200910187417 A CN200910187417 A CN 200910187417A CN 101658252 A CN101658252 A CN 101658252A
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- bacillus
- stichopus japonicus
- lactic acid
- acid bacteria
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A40/00—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
- Y02A40/80—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in fisheries management
- Y02A40/81—Aquaculture, e.g. of fish
- Y02A40/818—Alternative feeds for fish, e.g. in aquacultures
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Abstract
The invention discloses a food organism additive for cultivating stichopus japonicus, which has various nutrient components and bioactivators, and has non-toxic side effect, can promote appetite, enhance resisting ability of diseases and improve feed utilization; lactic acid bacteria and bacillus carry out slant tube cultivation, liquid seed cultivation and three-level amplification culture respectively to prepare pure bactericide, the lactic acid bacteria and bacillus are mixed by 1:5-50 of weight ratio, and then cooled Chinese medicinal herb water-decoctum is added; the Chinese medicinal herb water-decoctum is that: 0.2-1 percent of Chinese-date, 0.1-0.5 percent of hawthorn, 0.2-0.5 percent of medlar and 0.05-0.2 percent of astragalus mongholicus are taken by weight percentage of total weight of bactericide, and then the Chinese-date, the hawthorn, the medlar and the astragalus mongholicus are smashed, and water equal to 5 times of volume of raw material is added, and the mixture issoaked for 30min, is boiled by smash and is kept o be boiling with soft fire, until the decoctum is decocted to be one third of original volume, and filter liquid is obtained after dregs of the decoction are filtered.
Description
Technical field:
The present invention discloses a kind of breed stichopus japonicus and uses feed addictive, especially a kind of multiple nutritional components and bioactivator of containing, can promote appetite, strengthen resistivity, the food organism additive for cultivating stichopus japonicus that improves efficiency of feed utilization and have no side effect disease.
Background technology:
Sea cucumber (sea cucumbers) belongs to the Echinodermata Holothuroidea, and the sea cucumber that distributes in China marine site has kind more than 140, and wherein with stichopus japonicus (Apostichopus japonicus) performance optimal yellow, the Bohai Sea, economic worth is higher.Along with the fast development of domestic apostichopus japonicus culture industry, a series of disease problems have appearred in the rapid expansion of cultured area and the running lack of standardization under the intensive culture mode in succession in the apostichopus japonicus culture process, very urgent to the Prevention Research of stichopus japonicus disease.Research finds, it mainly is to be caused by bacterial pathogen that stichopus japonicus auricularia in seedling stage rots the self-dissolving illnesss such as (being commonly called as " change plate ") that limit, skin ulcer cause; Skin ulcer, Beancurd sheet, oedema that the mature stage sea cucumber occurs then cause (Wang et al.2003) by multiple cause of diseases such as bacterium, mould and parasites.The stichopus japonicus young " change plate " disease can cause the death rate of stichopus japonicus 80% and even 100%, and infection speed is fast, is widely current in Shandong, the nursery of each department, Liaoning, has caused serious economy loss.
At present, a series of alarming problems of meeting generation are operated: as the emerge in multitude of antibody-resistant bacterium, facilitate new pandemic like this for preventing and treating the stichopus japonicus morbidity and use antibiotic in a large number in part apostichopus japonicus culture family; Destroy the animal body normal microflora, cause the microecological balance imbalance, influence the animal immune function, body disease-resistant is weakened; Antibiotic residual in the stichopus japonicus product threatens human body health.Though Chinese herb feed additive can reach the purpose that strengthens resistance against diseases by the immunity that improves body self, its application in aquatic livestock has more report.Yet the special-purpose Chinese herb feed additive of stichopus japonicus does not seldom also have about being exclusively used in the feed bio-additive of apostichopus japonicus culture up to now.
Summary of the invention:
The present invention is in order to solve the above-mentioned technical problem of existing in prior technology, a kind of multiple nutritional components and bioactivator of containing is provided, can promote appetite, strengthen resistivity, the food organism additive for cultivating stichopus japonicus that improves efficiency of feed utilization and have no side effect disease.
Technical solution of the present invention is: a kind of food organism additive for cultivating stichopus japonicus, it is characterized in that is with lactic acid bacteria and bacillus cultivates through test tube slant cultivation, liquid seeds respectively and pure microbial inoculum is prepared in three grades of expansion cultivations, agent of lactic acid bacteria is mixed according to weight ratio 1: 5～50 with the bacillus agent, add the Chinese herbal medicine water cooking liquid of cooling then; Described Chinese herbal medicine water cooking liquid is the date of getting microbial inoculum gross weight 0.2～1%, 0.1～0.5% hawthorn, 0.2～0.5% matrimony vine and 0.05～0.2% the Radix Astragali, then date, hawthorn, matrimony vine and the Radix Astragali are pulverized, add the water logging bubble 30min of 5 times of volumes of raw material, through big fire boil, little fire keeps boiling, be boiled into 1/3rd of original volume until decoction, the filtrate of the filtering dregs of a decoction.
Described lactic acid bacteria is at least a in Lactobacillus casei or the lactobacillus acidophilus; Described bacillus is at least a in bacillus subtilis or the bacillus cereus.
The present invention is the mix preparation of probio and Chinese herbal medicine, and Chinese herbal medicine provides needed selectivity substrate for probio, thereby improves the survival rate of probio, and then brings into play the joint effect of the two.Probio is by the activity of microbiotic composition of influence or bacterium colony, reach the purpose of regulating microbiota balance in the stichopus japonicus alimentary canal, simultaneously, can also promote stichopus japonicus body secretion antibacterial material, produce materials such as organic acid, lyase, hydrogen peroxide, suppress harmful bacterium and unhelpful bacterium, thereby strengthen body health, improve production capacity, also can promote the secretion of digestive ferment and synthesizing of vitamin, improve the activity of immunoglobulin (Ig) and macrophage, strengthen body's immunity and premunition.Chinese herbal medicine contains multiple nutritional components and bioactivator as a kind of natural resources in addition, can promote appetite, strengthen the resistivity to disease, improves efficiency of feed utilization etc. and has no side effect.
The specific embodiment:
According to art methods, cultivate by test tube slant cultivation, liquid seeds respectively and three grades of expansion cultivations with lactic acid bacteria (Lactobacillus casei or lactobacillus acidophilus), bacillus (bacillus subtilis or bacillus cereus), prepare the pure microbial inoculum of four kinds of bacterial strains.The suitable incubation time of several probiotics is 36～48h, and the appropriate media salinity is 10～20, and optimum growth temperature is 25～35 ℃.The optimal pH of lactic acid bacteria is 5.4, can grow in the big excursion of pH equally, and the bacillus optimal pH is 7.6～8, but can grow in pH is 6～9 scope.The bacterial classification of Lactobacillus casei, lactobacillus acidophilus, bacillus subtilis and bacillus cereus is all from national common micro-organisms culture presevation administrative center CGMCC, wherein Lactobacillus casei (Lactobacillus Casei) numbering 1.121, lactobacillus acidophilus (Lactobacillus Acidophilus) numbering 1.1854, bacillus subtilis (Bacillus subtilis) numbering 1.884, bacillus cereus (Bacilluslicheniformis) numbering 1.105.
With agent of lactic acid bacteria (one or both in Lactobacillus casei and the lactobacillus acidophilus) and bacillus agent (one or both in bacillus subtilis and the bacillus cereus), mix according to weight ratio 1: 5～50, make the beneficial bacterium preparation, and then add the Chinese herbal medicine water cooking liquid of cooling.The Chinese herbal medicine water cooking liquid is the date of getting probiotics gross weight 0.2～1%, 0.1～0.5% hawthorn, 0.2～0.5% matrimony vine and 0.05～0.2% the Radix Astragali, then date, hawthorn, matrimony vine and the Radix Astragali are pulverized and (or got the Chinese herbal medicine of pulverizing, weigh then), add the water logging bubble 30min of 5 times of volumes of herbal raw material, through big fire boil, little fire keeps boiling, be boiled into 1/3rd of former water volume until decoction, with the filtrate of the gauze filtering dregs of a decoction.
Through check, it is 2*10 that the embodiment of the invention contains useful bacteria concentration
9More than the cfu/ml.
Experimental result:
The test of laboratory rearing stichopus japonicus:
Blank group: the feed that does not only add any additives by the prior art feeding.
Experimental group 1: feeding adds the feed of the embodiment of the invention, and the ratio of addition and breeding water is 100ml/m
3
Experimental group 2: feeding adds the feed of existing microbe additive, and the ratio of addition and breeding water is 100ml/m
3
Experiment shows that the embodiment of the invention can improve the stichopus japonicus digestive enzyme activity, especially protease and diastatic activity, and positive role is arranged to improving the stichopus japonicus immunologic function.The prolease activity of experimental group 1 is 4.5 times of blank group contrast, is 2.1 times of experimental group 2; The amylase activity of experimental group 1 is 2.9 times of blank group, is significantly higher than experimental group 2 (p＜0.05); The superoxide dismutase of experimental group 1 and lysozyme activity all are significantly higher than blank group (p＜0.05).Attack the poison experiment 60 days raising experiment end backs with Vibrio splindidus, get 30 stichopus japonicus for every group, the pathogenic bacteria Vibrio splindidus is expelled in the stichopus japonicus body, ID is the 0.2ml/ head, and cumulative mortality of each group in malicious back 7 days attacked in record.The result shows that it is 56.7% that experimental group 1 finishes the back survival rate experiment in 7 days, and the stichopus japonicus survival rate of experimental group 2 is 13%, and the blank group is then all dead.
Stichopus japonicus seedling growth test under plant's condition:
On August 10th, 2008 to September 10, one stichopus japonicus nursery carried out each 25m in Wafangdian
3Nursery pond is chosen immediately altogether and is thrown embodiment of the invention 100ml conduct with feed after 3 nursery ponds change water every day, and all the other nursery ponds are organized in contrast.
Experiment shows that the stichopus japonicus survival rate of seedling of experimental group improves 41.33% than control group; The average gross weight of experimental group stichopus japonicus improves 42.92% than control group.Experimental session, temperature of fry nursing pool are between 25.5-27.5 ℃, and seedling of Apostichopus japonicus is under the heat stress condition, its search for food and active state all undesirable, and experimental group is obvious to the survival rate effect that improves seedling of Apostichopus japonicus, and survival rate improves 22.13%, and the average gross weight of stichopus japonicus improves 25.06%.
Claims (2)
1. food organism additive for cultivating stichopus japonicus, it is characterized in that is with lactic acid bacteria and bacillus cultivates through test tube slant cultivation, liquid seeds respectively and pure microbial inoculum is prepared in three grades of expansion cultivations, agent of lactic acid bacteria is mixed according to weight ratio 1: 5～50 with the bacillus agent, add the Chinese herbal medicine water cooking liquid of cooling then; Described Chinese herbal medicine water cooking liquid is the date of getting microbial inoculum gross weight 0.2～1%, 0.1～0.5% hawthorn, 0.2～0.5% matrimony vine and 0.05～0.2% the Radix Astragali, then date, hawthorn, matrimony vine and the Radix Astragali are pulverized, add the water logging bubble 30min of 5 times of volumes of raw material, through big fire boil, little fire keeps boiling, be boiled into 1/3rd of original volume until decoction, the filtrate of the filtering dregs of a decoction.
2. food organism additive for cultivating stichopus japonicus according to claim 1 is characterized in that described lactic acid bacteria is at least a in Lactobacillus casei or the lactobacillus acidophilus; Described bacillus is at least a in bacillus subtilis or the bacillus cereus.
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| Application Number | Priority Date | Filing Date | Title |
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| CN2009101874172A CN101658252B (en) | 2009-09-17 | 2009-09-17 | Food organism additive for cultivating stichopus japonicus |
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| CN2009101874172A CN101658252B (en) | 2009-09-17 | 2009-09-17 | Food organism additive for cultivating stichopus japonicus |
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| CN101658252A true CN101658252A (en) | 2010-03-03 |
| CN101658252B CN101658252B (en) | 2013-04-17 |
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Cited By (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102559533A (en) * | 2010-12-23 | 2012-07-11 | 中国水产科学研究院黄海水产研究所 | Bacillus atrophaeus, and preparation and application of bacillus atrophaeus |
| CN102559534A (en) * | 2010-12-23 | 2012-07-11 | 中国水产科学研究院黄海水产研究所 | Bacillus cereus, and preparation and application of bacillus cereus |
| CN103222554A (en) * | 2012-12-24 | 2013-07-31 | 大连海洋大学 | Compound Chinese herbal medicine additive for sea cucumber and preparation method thereof |
| CN105746884A (en) * | 2016-02-02 | 2016-07-13 | 武汉万德瑞生物技术有限公司 | Probiotic composition for animal feed as well as preparation method and application thereof |
| CN110916014A (en) * | 2019-11-26 | 2020-03-27 | 上海泓宝绿色水产股份有限公司 | Oral sunstroke prevention bacterium |
| CN111149951A (en) * | 2020-02-11 | 2020-05-15 | 烟台市海洋经济研究院 | Preparation method of active fermented feed for stichopus japonicus |
| CN111647523A (en) * | 2020-05-07 | 2020-09-11 | 中国水产科学研究院黄海水产研究所 | Probiotic composition for stichopus japonicus culture and application thereof |
| CN111676148A (en) * | 2020-05-07 | 2020-09-18 | 中国水产科学研究院黄海水产研究所 | Lactobacillus paracasei CSDN-6 and application thereof |
Family Cites Families (3)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN100353979C (en) * | 2005-09-29 | 2007-12-12 | 大连太平洋海珍品有限公司 | Chinese herbal medicinal immune intensifier mode from left-eyed flounder and right-eyed flounder |
| CN100581378C (en) * | 2006-08-01 | 2010-01-20 | 济南亿民动物药业有限公司 | Method for preparing fermented Chinese herbal medicine preparations for feed |
| CN101292709A (en) * | 2007-12-20 | 2008-10-29 | 刘蒙榕 | Matrimony vine nutrition health care pet feedstuff |
-
2009
- 2009-09-17 CN CN2009101874172A patent/CN101658252B/en not_active Expired - Fee Related
Cited By (11)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102559533A (en) * | 2010-12-23 | 2012-07-11 | 中国水产科学研究院黄海水产研究所 | Bacillus atrophaeus, and preparation and application of bacillus atrophaeus |
| CN102559534A (en) * | 2010-12-23 | 2012-07-11 | 中国水产科学研究院黄海水产研究所 | Bacillus cereus, and preparation and application of bacillus cereus |
| CN102559534B (en) * | 2010-12-23 | 2013-09-25 | 中国水产科学研究院黄海水产研究所 | Bacillus cereus, and preparation and application of bacillus cereus |
| CN102559533B (en) * | 2010-12-23 | 2014-03-26 | 中国水产科学研究院黄海水产研究所 | Bacillus atrophaeus, and preparation and application of bacillus atrophaeus |
| CN103222554A (en) * | 2012-12-24 | 2013-07-31 | 大连海洋大学 | Compound Chinese herbal medicine additive for sea cucumber and preparation method thereof |
| CN105746884A (en) * | 2016-02-02 | 2016-07-13 | 武汉万德瑞生物技术有限公司 | Probiotic composition for animal feed as well as preparation method and application thereof |
| CN110916014A (en) * | 2019-11-26 | 2020-03-27 | 上海泓宝绿色水产股份有限公司 | Oral sunstroke prevention bacterium |
| CN111149951A (en) * | 2020-02-11 | 2020-05-15 | 烟台市海洋经济研究院 | Preparation method of active fermented feed for stichopus japonicus |
| CN111647523A (en) * | 2020-05-07 | 2020-09-11 | 中国水产科学研究院黄海水产研究所 | Probiotic composition for stichopus japonicus culture and application thereof |
| CN111676148A (en) * | 2020-05-07 | 2020-09-18 | 中国水产科学研究院黄海水产研究所 | Lactobacillus paracasei CSDN-6 and application thereof |
| CN111676148B (en) * | 2020-05-07 | 2021-12-03 | 中国水产科学研究院黄海水产研究所 | Lactobacillus paracasei CSDN-6 and application thereof |
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| CN101658252B (en) | 2013-04-17 |
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