CN110916014A - Oral sunstroke prevention bacterium - Google Patents
Oral sunstroke prevention bacterium Download PDFInfo
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- CN110916014A CN110916014A CN201911174570.1A CN201911174570A CN110916014A CN 110916014 A CN110916014 A CN 110916014A CN 201911174570 A CN201911174570 A CN 201911174570A CN 110916014 A CN110916014 A CN 110916014A
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- component
- oral
- sunstroke prevention
- prevention bacterium
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K50/00—Feeding-stuffs specially adapted for particular animals
- A23K50/80—Feeding-stuffs specially adapted for particular animals for aquatic animals, e.g. fish, crustaceans or molluscs
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K10/00—Animal feeding-stuffs
- A23K10/10—Animal feeding-stuffs obtained by microbiological or biochemical processes
- A23K10/16—Addition of microorganisms or extracts thereof, e.g. single-cell proteins, to feeding-stuff compositions
- A23K10/18—Addition of microorganisms or extracts thereof, e.g. single-cell proteins, to feeding-stuff compositions of live microorganisms
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K10/00—Animal feeding-stuffs
- A23K10/30—Animal feeding-stuffs from material of plant origin, e.g. roots, seeds or hay; from material of fungal origin, e.g. mushrooms
Abstract
The invention provides an oral heatstroke prevention bacterium which is characterized by being prepared by uniformly mixing, crushing and filtering the following component I, component II and component III by using a screen: wherein the component I is a composite strain synthetic material; the second component is a mixture of leguminous plants and zingiberaceae plants; the third component is a mixture of more than five herbaceous plants; the mass ratio of the first component to the second component to the third component is 7:2: 1. After the compound feed is fed, the appetite of aquatic animals in a high-water-temperature environment can be obviously improved, the physique can be enhanced, and the heatstroke resistance can be improved.
Description
Technical Field
The invention relates to the field of aquatic animal feed, in particular to an oral heatstroke prevention bacterium.
Background
The heat energy brought by the global temperature rise has led to the frequent occurrence of disasters such as large-scale or even ultra-large-scale typhoons, hurricanes, rainstorms, intense heat and the like. Annual climatic disasters of aquaculture are increasing. In recent years, the breeding disasters of sea cucumbers, river crabs and the like caused by severe summer heat bring huge economic losses to breeders.
Aiming at the problem that how to improve the summer heat resistance and stress resistance of aquatic animals in a severe summer heat environment, namely how to promote the appetite of the aquatic animals in a high-heat environment, strengthen the physique and improve the summer heat resistance is the problem to be solved at present.
Disclosure of Invention
The invention aims to overcome the defects and provide the heatstroke-resisting bacterium which can obviously improve the appetite of aquatic animals in a high-water-temperature environment, strengthen the physique and improve the heatstroke resistance when being fed with the compound feed.
The invention provides an oral sunstroke prevention bacterium which is characterized by being prepared by uniformly mixing, crushing and filtering the following component I, component II and component III by a screen mesh:
wherein, the component one is a composite strain synthetic material;
the second component is mixture of leguminous plant and zingiberaceae plant;
the third component is a mixture of more than five herbaceous plants;
the mass ratio of the first component to the second component to the third component is 5-7:1-5: 1.
Furthermore, the invention provides an oral sunstroke prevention bacterium, which is characterized in that: the component one is formed by mixing compound strains, aerobic bacteria and zeolite powder;
the mass part ratio of the composite strain, the aerobic bacteria and the zeolite powder is 20-35: 1: 50-80 parts;
wherein the composite strain is a fermentation product of EM strain.
The EM strain in the invention can be a mixture of more than one of rhodopseudomonas palustris, lactobacillus acidophilus, brewer's yeast, aspergillus actinomycete and bacillus subtilis.
Furthermore, the invention provides an oral sunstroke prevention bacterium, which is characterized in that: the preparation method of the composite strain is as follows: taking EM strain and saline water according to the ratio of 8-20: 1, pouring into a fermentation tank for fermentation, keeping the temperature at 25-30 ℃, and carrying out anaerobic fermentation culture for 4-10 days to obtain the composite strain.
Furthermore, the invention provides an oral sunstroke prevention bacterium, which is characterized in that: the saline water is sodium chloride and/or potassium chloride solution with mass concentration of 1-5%.
Furthermore, the invention provides an oral sunstroke prevention bacterium, which is characterized in that: the mass ratio of the leguminous plants to the zingiberaceae plants is 1: 2-5.
Furthermore, the invention provides an oral sunstroke prevention bacterium, which is characterized in that: the above Leguminosae plant is selected from radix Sophorae Flavescentis.
Furthermore, the invention provides an oral sunstroke prevention bacterium, which is characterized in that: the herbal mixture is selected from two or more of herbal plants containing menthol, decanoyl acetaldehyde, flavonoid, alkaloid, or biological enzyme.
Furthermore, the invention provides an oral sunstroke prevention bacterium, which is characterized in that: the herbal mixture is selected from two or more of herba Menthae, herba Houttuyniae, cortex Lycii, herba plantaginis, folium Mori, lalang grass rhizome, folium Bambusae, herba Bidentis Bipinnatae, and herba Solidaginis.
Furthermore, the invention provides an oral sunstroke prevention bacterium, which is characterized in that: the herbaceous plants are used in equal amount in the above herbaceous plant mixture.
Furthermore, the invention provides an oral sunstroke prevention bacterium, which is characterized in that: is used for compounding with seawater/fresh water animal feed;
500 g of heatstroke-resisting bacteria is used per 50-100 kg of animal feed.
The invention has the following functions and effects:
the invention is suitable for all seawater and fresh water fish, shrimp, crab, shellfish, sea cucumber breeding ponds and culture ponds (including mixed culture ponds), and is particularly suitable for culturing aged water bodies which have more sludge, generate 'white turbid water', 'rust water', 'black smelly water', 'old green water' and cause poor appetite, malnutrition, abnormal body color and slow growth of aquatic animals for many years.
The heatstroke prevention bacterium is developed for solving the great harm of the heatstroke temperature in summer to the aquaculture industry. The product is prepared by screening genuine materials capable of improving the heatstroke and stress resistance of aquatic animals in natural herbaceous plants, culturing in liquid or solid state fermentation, and adding special herbaceous plant adjuvants.
The invention promotes the aquatic animals to absorb and utilize functional substances of relieving summer heat, reducing heat, resisting stress and the like in the herbaceous plants by adjusting the structure of dominant microecological florae in the intestinal tracts of the aquatic animals, thereby enhancing the high tolerance of the aquatic animals to the temperature rise of the pond in summer heat seasons.
The compound feed disclosed by the invention can obviously improve the appetite of aquatic animals in a high-water-temperature environment, strengthen the physique and improve the heatstroke resistance when being fed.
Detailed Description
Examples 1,
The preparation method of the oral sunstroke prevention bacterium 1# product provided in this embodiment 1 is as follows:
1. preparing a composite strain: mixing EM strain (8-12 parts of Rhodopseudomonas palustris, 4-6 parts of Lactobacillus acidophilus, 1-3 parts of brewer's yeast, 1-2 parts of Actinomyces Monascus and 1-3 parts of Bacillus subtilis), wherein the content of the strain is 30 × 108cfu/ml or more; lactobacillus acidophilus in lactobacillus with strain content of 5 × 108cfu/ml or more; the Saccharomyces cerevisiae has strain content of 20 × 108cfu/ml or more; the content of actinomycete in filamentous fungi is 5 × 106cfu/ml or more) with 1% light saline according to 10: 1, fermenting in a fermentation tank, keeping the temperature at 25-30 ℃, and performing anaerobic fermentation culture for 10 days to prepare the composite strain for later use.
2. Taking 30% of powder bacillus natto, 1% of compound bacteria (prepared in the first step) and 69% of zeolite powder, mixing, drying and preparing into synthetic material for later use.
3. Taking the sophora flavescens powder and the ginger powder, compounding and mixing according to the proportion of 1:3, and fully and uniformly stirring for later use.
4. Mixing herba Menthae, herba Houttuyniae, cortex Lycii, herba plantaginis, folium Mori, lalang grass rhizome, folium Bambusae, herba Bidentis Bipinnatae, and herba Solidaginis at a ratio of 1:1:1:1:1:1:1:1, and stirring.
5. Compounding the components obtained in the steps ②, ③ and ④ according to the proportion of 7:2:1, and fully and uniformly stirring.
6. The mixture obtained in step ⑤ is well stirred, crushed, filtered by a screen and packaged.
Examples 2,
The preparation method of the oral sunstroke prevention bacterium 2# product provided in this embodiment 2 is as follows:
1. preparing a composite strain: taking EM strain and 5% light saline according to the ratio of 8: 1, fermenting in a fermentation tank, keeping the temperature at 25-30 ℃, and performing anaerobic fermentation culture for 8 days to prepare the composite strain for later use.
2. Taking 28% of powder bacillus natto, 1% of compound bacteria (prepared in the first step) and 71% of zeolite powder, mixing, drying and preparing into a synthetic material for later use.
3. Mixing radix Sophorae Flavescentis powder and rhizoma Zingiberis recens powder at a ratio of 1:2, and stirring.
4. Mixing herba Menthae, herba Houttuyniae, cortex Lycii, herba plantaginis, folium Mori, lalang grass rhizome, folium Bambusae, herba Bidentis Bipinnatae, and herba Solidaginis at a ratio of 1:1:1:1:1:1:1:1, and stirring.
5. Compounding the components obtained in the steps ②, ③ and ④ according to the proportion of 5:2:1, and fully and uniformly stirring.
6. The mixture obtained in step ⑤ is well stirred, crushed, filtered by a screen and packaged.
Examples 3,
The preparation method of the oral sunstroke prevention bacterium 3# product provided in this embodiment 3 is as follows:
1. preparing a composite strain: taking EM strain and 1% light saline according to the ratio of 20: 1, fermenting in a fermentation tank, keeping the temperature at 25-30 ℃, and carrying out anaerobic fermentation culture for 4 days to prepare the composite strain for later use.
2. Taking 20% of powder bacillus natto, 1% of compound bacteria (prepared in the first step) and 79% of zeolite powder, mixing, drying and preparing into a synthetic material for later use.
3. Taking the sophora flavescens powder and the ginger powder, compounding and mixing according to the proportion of 1:5, and fully and uniformly stirring for later use.
4. Mixing herba Menthae, herba Houttuyniae, cortex Lycii, herba plantaginis, folium Mori, lalang grass rhizome, folium Bambusae, herba Bidentis Bipinnatae, and herba Solidaginis at a ratio of 1:1:1:1:1:1:1:1, and stirring.
5. Compounding the components obtained in the steps ②, ③ and ④ according to the proportion of 7:5:1, and fully and uniformly stirring.
6. The mixture obtained in step ⑤ is well stirred, crushed, filtered by a screen and packaged.
Examples 4,
The preparation method of the oral sunstroke prevention bacterium 4# product provided in this embodiment 4 is as follows:
1. preparing a composite strain: taking EM strain and 2% light saline according to the ratio of 8: 1, fermenting in a fermentation tank, keeping the temperature at 25-30 ℃, and carrying out anaerobic fermentation culture for 5 days to prepare the composite strain for later use.
2. Taking 35% of powder bacillus natto, 1% of compound bacteria (prepared in the first step) and 64% of zeolite powder, mixing, drying and preparing into a synthetic material for later use.
3. Taking the sophora flavescens powder and the ginger powder, compounding and mixing according to the proportion of 1:3, and fully and uniformly stirring for later use.
4. Mixing herba Menthae, herba Houttuyniae, cortex Lycii, herba plantaginis, folium Mori, lalang grass rhizome, folium Bambusae, herba Bidentis Bipinnatae, and herba Solidaginis at a ratio of 1:1:1:1:1:1:1:1, and stirring.
5. Compounding the components obtained in the steps ②, ③ and ④ according to the proportion of 7:3:1, and fully and uniformly stirring.
6. The mixture obtained in step ⑤ is well stirred, crushed, filtered by a screen and packaged.
The products of the above embodiments: all are yellow brown powder, and have pungent taste.
The application range of the products of the above embodiments is as follows: is suitable for all seawater and fresh water fish, shrimp, crab, shellfish, sea cucumber and the like, and is especially suitable for culturing aged water bodies with more sludge, white turbid water, rust water, black foul water and old green water which cause poor appetite, malnutrition, abnormal body color and slow growth of aquatic animals for years, and the probiotic content is 150 multiplied by 108cfu/g。
The usage of the products of the above embodiments: the feed is fed by the compound feed, and 500 g of the feed is used for every 50-100 kg of the feed.
The notes on the products of the above examples are as follows:
1. chemical medicines and antibiotic medicines with sterilizing effect are forbidden to be used within three days before and five days after the administration of the heatstroke prevention medicines;
2. the product is not affected by weather and water temperature, and can ensure good water quality, resist summer heat, remove toxic substances, and promote growth;
3. the product is safe to use, and can be used twice when the water quality is seriously aged.
Experimental example of sunstroke prevention:
water body treatment by experiment: taking a hairy crab culture area of a high pure area in Nanjing city of Jiangsu province and two adjacent larval crab culture ponds of about 1 mu, adopting a fine culture feeding mode, wherein the water depth is about 1.2 m, the culture ponds are made of fine sand mud, the pond bottoms are clean, the pond slopes are flat, and the gradient ratio is 1:3, arranging water inlet and outlet gates at two ends of the cultivation pool, and installing a blocking and protecting net so as to prevent escape and exit from the pool. The blocking and protecting net is made of nylon bolting silk, the periphery of the cultivating pool is provided with escape-proof walls, the height of the walls is 30-40 cm, the walls are made of plastic film materials, the detained crabs can be effectively prevented from escaping, the water color is light green, the crabs do not die, the eating is normal, the detained crabs are divided into an experimental group and a control group, and the observation period is 7 months and 15 days to 8 months and 15 days.
The stocking condition and the pond condition are that 500 g of seedlings are put in each mu of the two ponds, the individual sizes are uniform, namely about 10 thousands of seedlings are planted in the two ponds, and the waterweeds are mainly planted in the ponds.
Problem analysis: the influence on the survival rate and the weight gain rate of the crabs after matched use of sunstroke prevention bacteria when the crabs are fed with the feed in the whole pond in the high-temperature period.
Treatment protocol
Experimental groups: the compound feed can be used for 50 kg of feed, and 500 g of sunstroke-resisting bacteria is 1 #.
Control group: without using sunstroke-resisting bacteria
The other feeding conditions and feeding amount are the same
Follow-up results after treatment:
average weight of 100 juvenile crabs obtained by sampling each time
Case analysis:
through observation of the whole experimental process, the following conclusions can be drawn:
firstly, the growth speed of the crabs is obviously accelerated
During the high temperature period, the growth speed of the experimental group crabs is obviously accelerated compared with that of the control group crabs, and the final average weight of the experimental group crabs is increased by 20.8% compared with that of the control group crabs.
(II) the survival rate of the crabs is obviously improved
During the high temperature period, the survival rate of the crabs subjected to sunstroke prevention in the experimental group can be improved by 4.81 percent compared with that of the control group.
In conclusion, the sunstroke prevention bacteria can promote the absorption and utilization of functional substances of the aquatic animals, such as relieving summer heat, reducing fever, resisting stress and the like, in the herbaceous plants by regulating the structure of the advantageous microecological flora in the intestinal tracts of the aquatic animals, thereby increasing the number of the sunstroke prevention bacteria
The same situation occurs with marine animals.
In addition, the same results as described above were obtained with the products of examples 2 to 4.
Claims (10)
1. The oral sunstroke prevention bacterium is characterized by being prepared by uniformly mixing, crushing and filtering the following component I, component II and component III by a screen mesh:
wherein the component I is a composite strain synthetic material;
the second component is a mixture of leguminous plants and zingiberaceae plants;
the third component is a mixture of more than five herbaceous plants;
the mass ratio of the first component to the second component to the third component is 5-7:1-5: 1.
2. An oral sunstroke prevention bacterium as claimed in claim 1, wherein:
the component one is formed by mixing a composite strain, aerobic bacteria and zeolite powder;
the mass part ratio of the composite strain, the aerobic bacteria and the zeolite powder is 20-35: 1: 50-80 parts;
wherein the composite strain is a fermentation product of EM strain.
3. An oral sunstroke prevention bacterium as claimed in claim 2, wherein:
the preparation method of the composite strain is as follows: taking EM strain and saline water according to the ratio of 8-20: 1, pouring into a fermentation tank for fermentation, keeping the temperature at 25-30 ℃, and carrying out anaerobic fermentation culture for 4-10 days to obtain the composite strain.
4. An oral sunstroke prevention bacterium as claimed in claim 3, wherein:
the saline water is a sodium chloride and/or potassium chloride solution with the mass concentration of 1-5%.
5. An oral sunstroke prevention bacterium as claimed in claim 1, wherein:
the mass ratio of the leguminous plants to the zingiberaceae plants is 1: 2-5.
6. An oral sunstroke prevention bacterium as claimed in claim 5, wherein:
the plant of Leguminosae is selected from radix Sophorae Flavescentis.
7. An oral sunstroke prevention bacterium as claimed in claim 1, wherein:
the herbal mixture is selected from more than five kinds of herbal plants containing menthol, decanoyl acetaldehyde, flavonoid, alkaloid or biological enzyme.
8. An oral sunstroke prevention bacterium as claimed in claim 1, wherein:
the herbal mixture is selected from the group consisting of peppermint, houttuynia, cortex Lycii, plantain, mulberry leaf, cogongrass rhizome, lophatherum gracile, Bidens bipinnata, and herba Solidaginis.
9. An oral sunstroke prevention bacterium as claimed in claim 1, wherein:
the herbaceous plants in the herbaceous plant mixture are used in equal amount.
10. An oral sunstroke prevention bacterium as claimed in any one of claims 1 to 9, wherein:
is used for compounding with seawater/fresh water animal feed;
500 g of heatstroke-resisting bacteria is used per 50-100 kg of animal feed.
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| CN201911174570.1A CN110916014A (en) | 2019-11-26 | 2019-11-26 | Oral sunstroke prevention bacterium |
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| CN201911174570.1A CN110916014A (en) | 2019-11-26 | 2019-11-26 | Oral sunstroke prevention bacterium |
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| CN110916014A true CN110916014A (en) | 2020-03-27 |
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| CN201911174570.1A Pending CN110916014A (en) | 2019-11-26 | 2019-11-26 | Oral sunstroke prevention bacterium |
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Citations (6)
| Publication number | Priority date | Publication date | Assignee | Title |
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| WO2004084923A1 (en) * | 2003-03-26 | 2004-10-07 | Takeda Food Products, Ltd. | Antistress agent |
| CN101658252A (en) * | 2009-09-17 | 2010-03-03 | 大连水产学院 | Food organism additive for cultivating stichopus japonicus |
| CN103960475A (en) * | 2014-05-16 | 2014-08-06 | 四川农业大学 | Livestock heat stress-resisting Chinese herbal medicine additive |
| CN105166551A (en) * | 2015-09-29 | 2015-12-23 | 通威股份有限公司 | Fodder capable of improving stress capability of grass carp |
| CN108126183A (en) * | 2018-01-05 | 2018-06-08 | 中国水产科学研究院长江水产研究所 | Chinese herbal and crude drugs preparations of aquatic livestock anti-heat stress and preparation method thereof |
| CN109198265A (en) * | 2018-09-26 | 2019-01-15 | 佛山市顺德区旺海饲料实业有限公司 | A kind of megathermal period food calling promotees the largemouth bass expanded pellet diet and preparation method thereof of digestion |
-
2019
- 2019-11-26 CN CN201911174570.1A patent/CN110916014A/en active Pending
Patent Citations (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2004084923A1 (en) * | 2003-03-26 | 2004-10-07 | Takeda Food Products, Ltd. | Antistress agent |
| CN101658252A (en) * | 2009-09-17 | 2010-03-03 | 大连水产学院 | Food organism additive for cultivating stichopus japonicus |
| CN103960475A (en) * | 2014-05-16 | 2014-08-06 | 四川农业大学 | Livestock heat stress-resisting Chinese herbal medicine additive |
| CN105166551A (en) * | 2015-09-29 | 2015-12-23 | 通威股份有限公司 | Fodder capable of improving stress capability of grass carp |
| CN108126183A (en) * | 2018-01-05 | 2018-06-08 | 中国水产科学研究院长江水产研究所 | Chinese herbal and crude drugs preparations of aquatic livestock anti-heat stress and preparation method thereof |
| CN109198265A (en) * | 2018-09-26 | 2019-01-15 | 佛山市顺德区旺海饲料实业有限公司 | A kind of megathermal period food calling promotees the largemouth bass expanded pellet diet and preparation method thereof of digestion |
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