CN105166551A - Fodder capable of improving stress capability of grass carp - Google Patents

Fodder capable of improving stress capability of grass carp Download PDF

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Publication number
CN105166551A
CN105166551A CN201510629675.7A CN201510629675A CN105166551A CN 105166551 A CN105166551 A CN 105166551A CN 201510629675 A CN201510629675 A CN 201510629675A CN 105166551 A CN105166551 A CN 105166551A
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China
Prior art keywords
parts
grass carp
feed
dregs
stress ability
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CN201510629675.7A
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Chinese (zh)
Inventor
卞世保
张璐
陈效儒
米海峰
李伟
李宝圣
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通威股份有限公司
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Priority to CN201510629675.7A priority Critical patent/CN105166551A/en
Publication of CN105166551A publication Critical patent/CN105166551A/en

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Abstract

The invention discloses a fodder capable of improving the stress capability of grass carp. The fodder is prepared from, by weight, 10-30 parts of soybean meal, 20-40 parts of rapeseed meal, 5-20 parts of DDGS, 5-20 parts of bacillus natto leavening, 3-15 parts of rice washing bran, 5-15 parts of flour, 2-3 parts of monocalcium phosphate, 1.5-3 parts of bentonite, 0.03-0.05 part of mildew preventive, 0.03-0.05 part of allicin, 0.2-0.3 part of complex vitamin, 0.03-0.05 part of inositol, 0.05-0.1 part of vitamin C and 1-2 parts of composite mineral. The fodder can effectively improve the stress capability of the grass carp while providing the grass carp with the needed nutrient substances, the death rate at high temperature and in the transportation process is reduced, the economic benefits of raising households are increased, and sustainable development of the aquaculture industry can be achieved.

Description

A kind of feed improving grass carp stress ability
Technical field
The present invention relates to technical field of aquaculture, particularly relate to a kind of feed improving grass carp stress ability.
Background technology
Grass carp is a kind of typical herbivorous fishes, and food chain is short, is one of topmost cultured freshwater fish of China, the inland river that its distributing region is mainly Chinese, North gets Heilungkiang, Southeast Asia, reaches Hainan Island in the south, extends to Thailand, Vietnam.Grass carp oneself is introduced a fine variety to all over the world, as states such as Japan, Southeast Asia, Eastern Europe, the U.S., nutritious with it, meat flavour is delicious, growth is fast, feed source is wide, the food consumption of low cost, the advantage such as in demand are subject to extensive welcome.But along with water pollution be on the rise, the continuous increase of cultivation scale, density, environment worsens coercing of cultivation grass carp more and more serious, in recent years cultivate grass carp due to environment-stress cause stress be more and more obvious, especially the 5-9 month, along with the rising of temperature, sell in fish process and occur that hemorrhage, scale loosens, mucus dropping situations phenomenon is general; In addition, drawing in the net to fish for, transport and support temporarily in process, stress reaction is also comparatively serious, even causes the higher death rate.After stress referring to that body is subject to the factor overstimulation such as biology, physics and chemistry existed in extraneous and internal environment, a series of non-specific whole body adaptation reaction produced immediately.For raising fish, common stress factor except the factors such as low temperature, high temperature, low dissolved oxygen, water pollutions, pathogenic microorganism, parasite, in addition crowded, fish for, the factor such as transport.Stress factor can cause the orthosympathetic excitement of fish, and in blood, catecholamine-adrenaline and norepinephrine concentration raise, and then Blood cortisol, cortisone, cortisone isoconcentration increase.Fish behavior is abnormal, and grazing rate and bait utilization ratio decline, and growth is suppressed, and decline to the resistance of disease simultaneously, has had a strong impact on the economic benefit of grass carp cultivation.
Along with the development of fish meal industry, occur in prior art that part improves the feed of grass carp stress ability, if publication number is CN104738343A, name is called the patent of invention of " a kind of improve feed of grass carp anti-stress ability and immunocompetence and preparation method thereof ", feed disclosed in this patent, be made up of following raw material: sunflower benevolence cake 18-36 part, wheat tail powder 8-18 part, fish meal 1-5 part, cotton dregs 12-22 part, dregs of beans 14-22 part, garlic powder 0.1-0.6 part, salt 0.5-1.5 part, green tea powder 0.075-0.75 part, black tea powder 0.075-0.75 part.Preparation method comprises: pretreatment of raw material, semi-finished product, finished product.Significantly improve grass carp Immune Indexes in the case study on implementation of this patented feed active, but rear grass carp long-distance transportation terminates to cultivation situation stress not have concrete description.
And for example, publication number is CN104489396A, name is called the patent of invention of " a kind of resisting stress grass carp expanded pellet diet and preparation method thereof ", the feed of this patent, following raw material components is comprised: 1-4 part fish meal with parts by weight, 10-25 part dregs of beans, 20-35 part dish dregs of rice, 5-15 part cotton dregs, 5-13 part DDGS, 0-10 part rice bran, 15-25 part wheat, 0.2 part of Choline Chloride, 1.2-2 part calcium dihydrogen phosphate, ore deposit premix more than 0.5 part, 1-2 part soya-bean oil, 0.15 part of multidimensional premix, 0.01-0.03 part alanyl glutamine, 0.02 part of bile acid, 0.02-0.1 part is high temperature resistant phytase, 0.1-0.3 part tuber of dwarf lilyturf, 0.1-0.3 part eclipta, 0.1-0.3 part Radix Astragali.But use the application water surface of this patent in pond culture, still have slight red body and severe to fall stress phenomenon occurring of squama.This patent serve reduce stress proportion, but to fail to solve completely in grass carp harvesting transportation stress generation.
Summary of the invention
The present invention aims to provide a kind of feed improving grass carp stress ability, has the needs that can meet Growth of Grass Carps Ctenopharyngodon Idellus, can significantly improve again the advantage of grass carp anti-stress ability.
In order to realize foregoing invention object, technical scheme of the present invention is as follows:
Improve a feed for grass carp stress ability, it is characterized in that: comprise following raw material by weight: dregs of beans 10 ~ 30 parts, the dish dregs of rice 20 ~ 40 parts, DDGS5 ~ 20 part, fermenting bacillus natto thing 5 ~ 20 parts, washes 3 ~ 15 parts, rice bran, 5 ~ 15 parts, flour, calcium dihydrogen phosphate 2 ~ 3 parts, bentonite 1.5 ~ 3 parts, mould inhibitor 0.03 ~ 0.05 part, allicin 0.03 ~ 0.05 part, B B-complex 0.2 ~ 0.3 part, inositol 0.03 ~ 0.05 part, vitamin C 0.05 ~ 0.1 part, composite mineral matter 1 ~ 2 part.
Wherein, DDGS is the trade name of protein feed in vinasse, the distiller's dried grain namely containing soluble solids.
Further preferably, the feed of described raising grass carp stress ability, comprises following raw material by weight: dregs of beans 12 ~ 25 parts, the dish dregs of rice 25 ~ 35 parts, DDGS8 ~ 15 part, fermenting bacillus natto thing 10 ~ 15 parts, wash 5 ~ 12 parts, rice bran, 8 ~ 13 parts, flour, calcium dihydrogen phosphate 2 ~ 3 parts, bentonite 1.5 ~ 3 parts, mould inhibitor 0.03 ~ 0.05 part, allicin 0.03 ~ 0.05 part, B B-complex 0.2 ~ 0.3 part, inositol 0.03 ~ 0.05 part, vitamin C 0.05 ~ 0.1 part, composite mineral matter 1 ~ 2 part.
In described fermenting bacillus natto thing, the viable count of bafillus natto is 1.0 × 10 10cFU/g ~ 1.0 × 10 11cFU/g.
Described fermenting bacillus natto thing adopts Bacillus natto liquid, dregs of beans, wheat bran, distilled water in the ratio of 1:20:5:10, obtains after fermenting at normal temperatures 5 ~ 8 days.
Described mould inhibitor be mould must gram Midew preventive for feed, can buy in market and obtain.
The phytophage fish microelement pre-mix material that described composite mineral matter provides for Guangdong Tongwei Feed Co., Ltd., can buy in market and obtain.
The phytophage fish vitamin premix that described B B-complex provides for Guangdong Tongwei Feed Co., Ltd., can buy in market and obtain.
The phytophage fish microelement pre-mix material that Guangdong Tongwei Feed Co., Ltd. provides and vitamin premix be through long-term experiment, practical proof develop balanced in nutrition, promote immunity premix product, there is wide in variety, that content is sufficient advantage.
Bentonite in feed formula of the present invention has effect of three aspects, first, for animal provides nutrition and 20 kinds of elements such as Cu, Zn, Co, Sn, Mo, Ag, VNi, Se, Cr, As, Ba, Pb, Hg, Cd, P, Mn, Ca, Na, Fe, Mg of healthy growth, play and supplement and balanced action, improve efficiency of feed utilization, reduce feed cost; The second, because bentonite has certain adhesive effect, therefore in feed, the efficiency of granule strength and granulator can be improved, there is certain agglomeration resistance effect; 3rd, bentonite has certain adsorptivity, adsorbable water, pernicious gas, harmful bacteria, plays the effect suppressing intestines problem, can improve the digestion of animal and the process of metabolism.
Inositol in feed formula of the present invention is the normal material added in aquatic animal feed, can lipotropic factor and Porcine HGF, prevents gaseous distention and fatty liver, causes digestion time to extend, cortex breakage, poky problem.
The effect that natto fermentation thing adds is the content increasing bafillus natto and natto metabolin in feed, bafillus natto and natto metabolin, has thrombus dissolving, antibacterial, the digestibility that improves protein and antioxidation etc.Bafillus natto metabolin is used to be play bacillus suppressing the breeding of harmful intestinal tract bacteria, promoting grass carp nonspecific immunity ability in feed of grass carp formula of the present invention, Nattokinase plays thrombus dissolving, hypolipemic function simultaneously, and softening blood vessel avoids function that stress be hemorrhage.
Beneficial effect of the present invention:
(1) dregs of beans, the dish dregs of rice, DDGS in feed of the present invention composition, wash rice bran, flour and provide growth necessary nutriment for grass carp; Calcium dihydrogen phosphate can improve immunity and the meat of grass carp; Bentonite, for while grass carp provides nutritional labeling, improves digestive function, also can play the effect of agglomeration resistance to feed; Inositol can prevent the overnutrition of grass carp, produces the problem of fatty liver; Fermenting bacillus natto thing can improve stress ability and the immunity of grass carp; Allicin all has antibacterial action to multiple Gram-positive and negative bacterium, suppression killing action is all had to bacillus (shigella dysenteriae, typhoid bacillus, Escherichia coli, Bordetella pertussis), fungi (Candida albicans, cryptococcus, aspergillus fumigatus), virus (cytomegalovirus), Amoeba, Trichomonas vaginalis, pinworm etc., especially strong to the effect of the enteric bacteria such as Escherichia coli, shigella dysenteriae, in today that environmental pollution is day by day serious, to the effect playing sterilization in the body of grass carp, the death rate of grass carp can be reduced; Other compositions: B B-complex, composite mineral matter provide vitamin and mineral matter for grass carp, ensure its healthy growth, and to add an ascorbic object be ester oxidase in most fish lacks needed for synthetic vitamin C L-GuA, need to add vitamin C in feed, to ensure the normal physiological function of fish, the ability of the immunity of fish, premunition and anti allergic reaction also can be improved in addition.
To sum up, the present invention is selected and rational proportion design by specific raw material, make feed of the present invention can meet grass carp healthy growth, anti-stress ability and the immunocompetence of grass carp can be improved again, in megathermal period and transportation, reduce the death rate of grass carp, improve pond economic benefit, make aquaculture industry be able to sustainable development.
(2) as the present invention's preferred scheme further: the composition of feed comprises: dregs of beans 12 ~ 25 parts, the dish dregs of rice 25 ~ 35 parts, DDGS8 ~ 15 part, fermenting bacillus natto thing 10 ~ 15 parts, washes 5 ~ 12 parts, rice bran, 8 ~ 13 parts, flour, calcium dihydrogen phosphate 2 ~ 3 parts, bentonite 1.5 ~ 3 parts, mould inhibitor 0.03 ~ 0.05 part, allicin 0.03 ~ 0.05 part, B B-complex 0.2 ~ 0.3 part, inositol 0.03 ~ 0.05 part, vitamin C 0.05 ~ 0.1 part, composite mineral matter 1 ~ 2 part.In the program, the proportioning of each composition is more accurate, and anti-stress effect is better.
(3), in the present invention, in fermenting bacillus natto thing, the viable count of bafillus natto is 1.0 × 10 10cFU/g ~ 1.0 × 10 11cFU/g, to ensure the basal level of bafillus natto, guaranteeing can its due biological effect, namely suppress the breeding of harmful intestinal tract bacteria, promote grass carp nonspecific immunity ability, play thrombus dissolving, reducing blood lipid, softening blood vessel simultaneously, prevent stress be hemorrhage object.
Detailed description of the invention
Embodiment 1
Improve a feed for grass carp stress ability, comprise following raw material by weight: dregs of beans 100kg, dish dregs of rice 400kg, DDGS50kg, fermenting bacillus natto thing 200kg, washes rice bran 150kg, flour 50kg, calcium dihydrogen phosphate 30kg, bentonite 30kg, mould inhibitor 0.3kg, allicin 0.5kg, B B-complex 3kg, inositol 0.3kg, vitamin C 1kg, composite mineral matter 20kg.
Embodiment 2
Improve a feed for grass carp stress ability, comprise following raw material by weight: dregs of beans 300kg, dish dregs of rice 200kg, DDGS200kg, fermenting bacillus natto thing 50kg, washes rice bran 30kg, flour 150kg, calcium dihydrogen phosphate 20kg, bentonite 15kg, mould inhibitor 0.5kg, allicin 0.3kg, B B-complex 2kg, inositol 0.5kg, vitamin C 0.5kg, composite mineral matter 10kg.
Embodiment 3
Improve a feed for grass carp stress ability, comprise following raw material by weight: dregs of beans 120kg, dish dregs of rice 350kg, DDGS80kg, fermenting bacillus natto thing 150kg, washes rice bran 50kg, flour 130kg, calcium dihydrogen phosphate 20kg, bentonite 3kg, mould inhibitor 0.4kg, allicin 5kg, B B-complex 2.2kg, inositol 0.35kg, vitamin C 0.5kg, composite mineral matter 12kg.
Embodiment 4
Improve a feed for grass carp stress ability, comprise following raw material by weight: dregs of beans 250kg, dish dregs of rice 250kg, DDGS150kg, fermenting bacillus natto thing 50kg, washes rice bran 120kg, flour 80kg, calcium dihydrogen phosphate 30kg, bentonite 15kg, mould inhibitor 0.45kg, allicin 4kg, B B-complex 2kg, inositol 0.4kg, vitamin C 0.8kg, composite mineral matter 15kg.
In the present embodiment, in fermenting bacillus natto thing, the viable count of bafillus natto is 1 × 10 10cFU/g.
Embodiment 5
Improve a feed for grass carp stress ability, comprise following raw material by weight: dregs of beans 250kg, dish dregs of rice 280kg, DDGS120kg, fermenting bacillus natto thing 120kg, washes rice bran 50kg, flour 100kg, calcium dihydrogen phosphate 30kg, bentonite 25.6kg, mould inhibitor 0.45kg, allicin 0.3kg, B B-complex 3kg, inositol 0.35kg, vitamin C 0.5kg, composite mineral matter 20kg.
In the present embodiment, fermenting bacillus natto thing adopts Bacillus natto liquid, dregs of beans, wheat bran, distilled water in the ratio of 1:20:5:10, and obtain after 8 days through normal temperature fermentation, during use, in fermenting bacillus natto thing, the viable count of bafillus natto is 1 × 10 11cFU/g.
Embodiment 6
Improve a feed for grass carp stress ability, comprise following raw material by weight: dregs of beans 110kg, dish dregs of rice 350kg, DDGS120kg, fermenting bacillus natto thing 120kg, washes rice bran 50kg, flour 100kg, calcium dihydrogen phosphate 20kg, bentonite 25.6kg, mould inhibitor 0.3kg, allicin 0.3kg, B B-complex 3kg, inositol 0.3kg, vitamin C 0.5kg, composite mineral matter 20kg.
In the present embodiment, fermenting bacillus natto thing adopts Bacillus natto liquid, dregs of beans, wheat bran, distilled water in the ratio of 1:20:5:10, and obtain after 5 days through normal temperature fermentation, during use, in fermenting bacillus natto thing, the viable count of bafillus natto is 6 × 10 10cFU/g; The mould inhibitor of the present embodiment be mould must gram Midew preventive for feed.
Embodiment 7
Improve a feed for grass carp stress ability, comprise following raw material by weight: dregs of beans 225kg, dish dregs of rice 320kg, DDGS100kg, fermenting bacillus natto thing 120kg, washes rice bran 80kg, flour 100kg, calcium dihydrogen phosphate 25kg, bentonite 20kg, mould inhibitor 0.3kg, allicin 0.4kg, B B-complex 2.5kg, inositol 0.3kg, vitamin C 0.8kg, composite mineral matter 15kg.
In the present embodiment, fermenting bacillus natto thing adopts Bacillus natto liquid, dregs of beans, wheat bran, distilled water in the ratio of 1:20:5:10, and obtain after 6 days through normal temperature fermentation, during use, in fermenting bacillus natto thing, the viable count of bafillus natto is 5 × 10 10cFU/g; The mould inhibitor of the present embodiment be mould must gram Midew preventive for feed; The phytophage fish microelement pre-mix material that composite mineral matter selects Guangdong Tongwei Feed Co., Ltd. to provide; The phytophage fish vitamin premix that B B-complex selects Guangdong Tongwei Feed Co., Ltd. to provide.
Embodiment 8
In order to beneficial effect of the present invention is better described, provide technical scheme culture experiment provided by the invention below.
This culture experiment from 15 days June in 2015, from end of day in August 8, choose to occur in grass carp transportation before June 15 hemorrhage, fall squama, 3 mouthfuls of ponds that mucus comes off.The feed that these 3 mouthfuls of pond grass carps eat is labeled as A, B, C respectively, is labeled as O according to the feed that this technological invention is prepared.Observe grass carp in three mouthfuls of fish ponds, feed the resisting stress after 18,20,25 days and long distance transportation death condition, and contrast superoxide dismutase activity in Grass Carp Serum, experimental result is in table 1,2,3.
Table 1: feed of the present invention and commercially available A feed affect contrast table to grass carp anti-stress ability and immune indexes
Table 2: feed of the present invention and commercially available B feed affect contrast table to grass carp anti-stress ability and immune indexes
Table 3: feed of the present invention and commercially available C feed affect contrast table to grass carp anti-stress ability and immune indexes
As can be seen from above-mentioned experiment, environment-friendly type grass carp immunization feedstuff cultivation grass carp of the present invention is after 18,20,25 days, grass carp hemorrhage, mucus come off and transport death rate situation and is better than use A, B, C Three feed far away, especially in Grass Carp Serum, superoxide dismutase activity promotes rapidly, superoxide dismutase is the scavenger of ultra-oxygen anion free radical, is one of key enzyme of body defenses oxidative stress.Grass carp resisting stress immunization feedstuff of the present invention solves the problem of selling grass carp stress ability difference in fish process, decreases the economic loss at experiment training family.Meanwhile, what use in this grass carp resisting stress immunization feedstuff is all the raw materials allowing in raw material catalogue to add, and avoids the impact that raiser abuses medicine or uses illegal drug to cause food security.The present invention is environmental protection, the novel aquatic feeds promoting grass carp anti-stress ability.
Illustrate: the index bleeding standard in above table is for visually observing, and fish body belly is rubescent, musculature is congested is standard; Mucus situation, passes judgment on finger touch, and during fish body harvesting, mucus is normal, and along with the prolongation of time after dried up, mucus reduces gradually, and with finger touch, fish body is completely coarse, and the time without smooth feeling is fish body rough time.

Claims (7)

1. improve a feed for grass carp stress ability, it is characterized in that: comprise following raw material by weight: dregs of beans 10 ~ 30 parts, the dish dregs of rice 20 ~ 40 parts, DDGS5 ~ 20 part, fermenting bacillus natto thing 5 ~ 20 parts, washes 3 ~ 15 parts, rice bran, 5 ~ 15 parts, flour, calcium dihydrogen phosphate 2 ~ 3 parts, bentonite 1.5 ~ 3 parts, mould inhibitor 0.03 ~ 0.05 part, allicin 0.03 ~ 0.05 part, B B-complex 0.2 ~ 0.3 part, inositol 0.03 ~ 0.05 part, vitamin C 0.05 ~ 0.1 part, composite mineral matter 1 ~ 2 part.
2. the feed improving grass carp stress ability as claimed in claim 1, it is characterized in that: comprise following raw material by weight: dregs of beans 12 ~ 25 parts, the dish dregs of rice 25 ~ 35 parts, DDGS8 ~ 15 part, fermenting bacillus natto thing 10 ~ 15 parts, wash 5 ~ 12 parts, rice bran, 8 ~ 13 parts, flour, calcium dihydrogen phosphate 2 ~ 3 parts, bentonite 1.5 ~ 3 parts, mould inhibitor 0.03 ~ 0.05 part, allicin 0.03 ~ 0.05 part, B B-complex 0.2 ~ 0.3 part, inositol 0.03 ~ 0.05 part, vitamin C 0.05 ~ 0.1 part, composite mineral matter 1 ~ 2 part.
3. the feed improving grass carp stress ability as claimed in claim 1 or 2, is characterized in that: in described fermenting bacillus natto thing, the viable count of bafillus natto is 1.0 × 10 10cFU/g ~ 1.0 × 10 11cFU/g.
4. the feed improving grass carp stress ability as claimed in claim 1 or 2, is characterized in that: described fermenting bacillus natto thing adopts Bacillus natto liquid, dregs of beans, wheat bran, distilled water in the ratio of 1:20:5:10, obtains after fermenting at normal temperatures 5 ~ 8 days.
5. the as claimed in claim 1 or 2 feed improving grass carp stress ability, is characterized in that: described mould inhibitor be mould must gram Midew preventive for feed.
6. the feed improving grass carp stress ability as claimed in claim 1 or 2, is characterized in that: the phytophage fish microelement pre-mix material that described composite mineral matter provides for Guangdong Tongwei Feed Co., Ltd..
7. the feed improving grass carp stress ability as claimed in claim 1 or 2, is characterized in that: the phytophage fish vitamin premix that described B B-complex provides for Guangdong Tongwei Feed Co., Ltd..
CN201510629675.7A 2015-09-29 2015-09-29 Fodder capable of improving stress capability of grass carp CN105166551A (en)

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Cited By (5)

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CN106719163A (en) * 2016-11-18 2017-05-31 蚌埠市金牛湾农业科技发展有限公司 A kind of prevention and controls of grass carp stress disease
CN106721516A (en) * 2016-11-24 2017-05-31 佛山市南海东方澳龙制药有限公司 A kind of aquatic products anti-stress feed and feed addictive
CN106923085A (en) * 2017-03-30 2017-07-07 江门海大饲料有限公司 It is a kind of to prevent functional feed of the red body of grass carp physiological and preparation method thereof
CN107114617A (en) * 2017-06-25 2017-09-01 广州市诚水产科技有限公司 A kind of grass carp compound premixed feed and its application
CN107347743A (en) * 2017-07-31 2017-11-17 湖南文理学院 Culter fish live transport keep-alive preservation methods

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Cited By (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN106719163A (en) * 2016-11-18 2017-05-31 蚌埠市金牛湾农业科技发展有限公司 A kind of prevention and controls of grass carp stress disease
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CN106923085A (en) * 2017-03-30 2017-07-07 江门海大饲料有限公司 It is a kind of to prevent functional feed of the red body of grass carp physiological and preparation method thereof
CN107114617A (en) * 2017-06-25 2017-09-01 广州市诚水产科技有限公司 A kind of grass carp compound premixed feed and its application
CN107347743A (en) * 2017-07-31 2017-11-17 湖南文理学院 Culter fish live transport keep-alive preservation methods
CN107347743B (en) * 2017-07-31 2020-03-27 湖南文理学院 Alive-keeping and fresh-keeping method for transporting alive whitefish living bodies

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Application publication date: 20151223