WO2004084923A1

WO2004084923A1 - Antistress agent

Info

Publication number: WO2004084923A1
Application number: PCT/JP2004/003898
Authority: WO
Inventors: Shunsuke Koshio; Yoshihiro Yamamoto
Original assignee: Takeda Food Products, Ltd.
Priority date: 2003-03-26
Filing date: 2004-03-22
Publication date: 2004-10-07
Also published as: JPWO2004084923A1; JP4813902B2

Abstract

An antistress agent for fishes and shellfishes characterized by containing dead lactic acid bacterium cells optionally having been processed. This antistress agent is useful in preventing or treating stresses in fishes and shellfishes.

Description

Anti-stress agent

Technical field

The present invention relates to an anti-stress agent that imparts resistance to seafood against the adverse effects of stress. The present invention also relates to an aquaculture method using the same.

In the past, when aquaculture and aquatic animals were bred for various seafood, these aquatic animals were in physical condition due to overcrowding of the rearing environment, temperature changes or deterioration of water quality, or stress such as transportation. It has been known that such problems as disintegration, fading and discoloration, decrease in fighting force against diseases, poor feeding, poor physique, and deterioration in meat quality and fat riding (Japanese Patent Laid-Open No. 0-9-30) 8 4 4 0). The following are examples of adverse effects caused by such stress.

As for the juvenile fish, there are attacking individuals (attacking individuals) and attacking individuals (inferior individuals) in the breeding aquarium, and the inferior individuals are placed under intense stress, which hinders weight gain (Heisei 8) Abstracts of Annual Meeting of the Japanese Society of Fisheries Science, p 6 4, 1 9 9 6).

As for chu, non-territory individuals who cannot have territory report a low growth rate compared to territory territory due to its narrow movement range and low feeding frequency. Abstracts of Annual Meeting of the Fisheries Society of Japan, p 4 3, 1 9 9 6) It is considered to be a stressed inferior individual with a low growth rate, as is the case with non-female individuals.

In addition, in a fish that is nervous, such as flounder, the feeding becomes worse due to the stress after changing the pond for sorting or cleaning the pond. Just like flounder, Feeding worsens after pond change.

In addition, ornamental fish lose their original vivid colors due to skin fade and discoloration caused by stress.

In order to prevent these adverse effects, drugs such as antibiotics have been widely used. However, antibiotics and other drugs are effective to some extent, but there are safety problems such as residues in animals and environmental pollution, and adverse effects due to the emergence of resistant bacteria. Heading in the direction. In addition to antibiotic preparations, viable bacteria are also known. Examples of viable bacteria include photosynthetic bacteria (PSB), mixed microorganisms (trade name “Cycle”: manufactured by Sudo Co., Ltd., trade name “Super Bio”: manufactured by Tomofuji Co., Ltd.), and the like. However, many of these viable bacteria agents are room temperature storage liquid types, and the viable cell retention rate before use is generally poor. For this reason, there are many cases where the number of viable bacteria is insufficient when used, or the effect is not recognized if the usage environment becomes too harsh, making it difficult to use under appropriate conditions. The things that can improve the constitution are not yet in practical use. As long as it is a viable cell, care must be taken to maintain the viable cell count, making it difficult to handle and process management. Disclosure of the invention

An object of the present invention is to provide a preparation for effectively preventing or treating stress in seafood.

As a result of intensive studies to achieve the above-mentioned problems, the present inventors have unexpectedly and surprisingly found that a dead cell of lactic acid bacteria or a processed product thereof is not stressed for fish and shellfish. It has been found that it has an excellent effect on the prevention or treatment of the disease. In addition, when the dead cells are used as active ingredients instead of living lactic acid bacteria, the present inventors can easily handle the active ingredients and process management easily when producing anti-stress agents. The active ingredient in the formulated product is highly stable during storage and in water I also learned that.

That is, the present invention

(1) An anti-stress agent for seafood characterized by containing dead lactic acid bacteria or a processed product thereof,

(2) Lactobacillus Lactobacillus plantarum, Lactobacillus acidophilus, Lactobacillus brevis, Lactobacillus casei, Lactobacillus casei, Lactobacillus casei, Lactobacillus casei, Lactobacillus casei, Lactobacillus casei, Lactobacillus casei fermentunu, Lactobacillus paracasei, Lactobacillus buchneri, Lactobacillus delbrueckii, Lactobacillus rhamnosus (Lactobacillus rhamnosus octo) thermophi lus, Enterococcus faecalis> Enterococcus faecium, Lactococcus lactis, Lactococcus plantarum (Lact) ococcus pi ant arum) or Bifidobacterium thermophi lum, Bifidobacterium longum Bifidobacterium breve Bifidobacterium breve A stress agent, (3) the anti-stress agent for fish and shellfish according to (1) or (2) above, wherein the dead cells of lactic acid bacteria are dead cells obtained by heat treatment of lactic acid bacteria,

(4) The above-mentioned (1) to (1), wherein the dead cells of lactic acid bacteria are heat-treated dead cells of Lactobacillus plantarumL-137 strain (Accession number FERM BP-08607) (3) the anti-stress agent for seafood described in any of the above,

(5) The above (1) characterized in that the seafood is farmed fish or ornamental fish ~ Antistress agent for fish and shellfish according to any one of (4),

(6) The antistress agent for seafood according to any one of the above (1) to (5), wherein the seafood is a saltwater fish or a freshwater fish,

(7) The antistress agent for seafood according to any one of (1) to (6) above, wherein the seafood is a larva

(8) The ratio of dead lactic acid bacteria or treated product thereof is 0.1 to 50% by mass relative to the total amount, according to any one of (1) to (7) above Anti-stress agent for seafood,

(9) Seafood feed comprising the antistress agent for fish and shellfish according to any one of (1) to (8) above,

(10) The feed for fish and shellfish according to (9) above, wherein the concentration of dead lactic acid bacteria or the processed product thereof in the feed is more than 0 and not more than 2000 ppm

(11) A method for preventing or treating seafood stress, comprising feeding the seafood with the antistress agent for seafood according to any one of (1) to (8) above, (12) Death of lactic acid bacteria A method for preventing or treating stress in seafood, characterized by feeding the body or processed product thereof to seafood at a rate of 0.0005 to 75 mg per day per kg of seafood;

(13) A method for suppressing a rise in serum cortisol in seafood, comprising using the antistress agent for seafood according to any one of (1) to (8) above,

(14) A method for cultivating seafood characterized by using the antistress agent for seafood described in (1) above,

(15) The aquaculture method described in (14) above, wherein the taste quality of seafood is improved, and

(16) The aquaculture method described in (14) or (15) above, wherein the survival rate of cultured seafood is improved,

About. BEST MODE FOR CARRYING OUT THE INVENTION

In the present invention, as the lactic acid bacteria, any known lactic acid bacteria such as Lactobacillus genus, Streptococcus genus, Enterococcus genus, Lactococcus genus or Bifidobacterium can be used. In more detail, Lactobacillus pi ant arum, Lactobacillus acidophilus, Lactobacillus brevis, Lactobacillus brevis, Lactobacillus casei, Lactobacillus casei, Lactobacillus fermentum, Lactobacillus paracasei, Lactobacillus buchneri, Lactobacillus buchneri, Lactobacillus delbruecki i, Lactobacillus delbruecki i, Lactobacillus Streptococcus thermophilus, Enterococcus faecalis, Enterococcus faecium, Lactococcus lactis, Lactococcus lactis (Lactococcus plantarum), Bifidobacterium thermophilum, Bifidobacterium longum, Bifidobacterium breve and the like. Of these various lactobacilli, Lactobacillus plantarum is preferred.

Lactobacillus plantarum L 1 137 can be mentioned as a representative of the bacteria belonging to Lactobacillus plantarum used in the present invention. This bacteria is an independent administrative agency, National Institute of Advanced Industrial Science and Technology. Deposited at the center under the deposit number FERM BP-08607 (transferred from FE RM P-15317 deposited on November 30, 1995). The Lactobacillus plantarum used in the present invention further includes Lactobacillus plantarum. (Lactobacillus pi ant arum) JCM 1 1 4 9 Reference strain and Lactobacillus plantarum L— 0 5 1 (Microbiken No. 1 1 9 1 2) are included. Lactobacillus planum lamb L— 1 3 7 is most preferred.

The bacterium used in the present invention can be obtained by culturing in a medium such as a natural medium, a synthetic medium, or a semi-synthetic medium. A medium containing a nitrogen source and a carbon source is used as the medium. Nitrogen sources include, for example, meat extract, peptone, gluten, casein, yeast extract, amino acids, etc., and carbon sources include, for example, glucose, xylose, flax 1 ^ 1s, wild boar, maltose, water Me, soup, starch, bacus, bran, molasses, glycerin, etc. are used. In addition, for example, ammonium sulfate, potassium phosphate, magnesium chloride, sodium chloride, iron, manganese, molybdenum, and the like can be added as inorganic substances, and various vitamins and the like can be added. The culture temperature is about 25 to 40 ° C, preferably about 27 to 35 ° C, the culture time is about 12 to 48 hours, and aeration and shaking may be performed. The pH of the medium is about 3-6, preferably about 4-6. After completion of the culture, the cells may be collected and then killed cells may be prepared, or the cells in the culture solution may be changed to dead cells without separating the cells from the culture solution. Bacteria may be collected. As a method for collecting the bacterial cells, for example, there is a method in which distilled water is added to the culture solution, the supernatant is removed by means such as centrifugation, the operation is repeated as necessary, and the bacterial cells are collected by centrifugation or filtration. . Dead cells can be obtained by inactivating the collected live cells or the culture medium containing the live cells, for example, by heating, ultraviolet irradiation, formalin treatment, or the like. When dead cells are obtained by heat treatment, the heating temperature is usually about 60 to 100 ° C., preferably about 70 to 9. The heating means may be a known means using a heater. The heating time is usually about 5 to 40 minutes, preferably about 10 to 30 minutes after reaching the desired temperature. The treated product of dead cells can be obtained, for example, by freeze-drying or spray-drying dead cells. In addition, add appropriate excipients such as dextrin and cellulose to dead cells, This can also be obtained by freeze drying or spray drying. The processed product of dead cells may also be an extract of dead cells.

The seafood in the present invention is not particularly limited, and may be any of freshwater fish, saltwater fish, and crustaceans, and may be any of cultured fish and ornamental fish. Examples of freshwater fish include eel, carp, rainbow trout, ayu, tilapia, crucian carp, goldfish (e.g., lunchyu, Japanese gold, comet, vermilion gold, Dutch shigashira, dice, etc.), guppy, apistogramma, and dish. Examples of saltwater fish include bluefin tuna, puri, Thailand, coho salmon, horse mackerel, flounder, flounder, blackfish, trough, and amberjack. In addition, salmon trout and the like can be mentioned as belonging to both freshwater fish and saltwater fish. Examples of crustaceans include prawns, black taiga, ushebi, kolayebi, and crab.

The seafood as described above may be any of larvae (including small fish, larvae, fry, and juveniles) and adult fish.

The anti-stress agent of the present invention may be a killed lactic acid bacterium or a processed product thereof, or a preparation obtained by mixing dead cells or a processed product thereof with an excipient or diluent. The blending ratio of dead cells or processed product thereof to the whole preparation is preferably about 0.1 to 95% by mass, more preferably about 0.1 to 50% by mass. Examples of the dosage form include pellets, tablets, liquids, powders, granules, and pastes. Such preparations can be brought into contact with seafood or given orally. Solid excipients or diluents include, for example, fish meal, bone meal, skim milk, cottonseed meal, wheat flour, wheat germ, rice bran, brewer's yeast, cellulose, vitamins (eg / 3-strength rotin, vitamin D ₃ , Menadione-sodium hydrogen sulfite, —tocopherol, thiamine—nitrate, riboflavin, pyridoxine—hydrochloride, cyanocobalamin, piotin, inositol, nicotinic acid, calcium pantothenate, folic acid, choline choline, paraaminobenzoic acid or vitamin C Etc.), mineral mixes (eg magnesium sulfate, disodium phosphate, 2-natto phosphate) And so-called feed materials such as potassium potassium, iron citrate, calcium lactate, aluminum hydroxide, sulfite sulfate, copper sulfate, manganese sulfate, calcium iodate or cobalt sulfate). Examples of the liquid excipient or diluent include water or a mixture of water and alcohol. The antistress agent of the present invention can be easily produced by mixing dead bacteria of Lactobacillus plantum or a processed product thereof with the above-mentioned excipient or diluent. When mixing, it may be heated as desired to obtain a flaky preparation. The preparation of the present invention thus produced may be used as a feed for seafood. When used as feed, the concentration of dead cells in the feed or processed product thereof is preferably more than about 0 ppm and not more than 200 ppm, more preferably from about 0.1 to 200 ppm. is there.

The feeding amount of the antistress agent for seafood of the present invention is 0.05 to 75 mg per day per kg body weight of the seafood in terms of the above-mentioned dead cells of lactic acid bacteria or processed products thereof. It is preferable that it is 0.05 to 7.5 mg.

One possible mechanism for the anti-stress effect of the preparation of the present invention on fish and shellfish is to suppress the rise in serum cortisol. In the event of a small stress, the neuro-endocrine system first responds to stress and secretes neurotransmitters and hormones. Physiological effects of secreted neurotransmitters and hormones can cause physiological changes in the body, which can lead to growth inhibition, reproductive function and decreased resistance to disease. The effects of these stresses are the result of the integration of various substances such as neurotransmitters and hormones, but it is said that cortisol plays the leading role (cultured 2001. No. 1 1, 56- 59). Therefore, it is possible to resist the adverse effects of stress as described above by suppressing the rise in cortisol.

The antistress agent for seafood of the present invention has an action of suppressing the rise of cortisol in seafood, and when administered to seafood, the stress of seafood can be effectively prevented or treated. As a result, the weight of seafood increases, the growth of seafood increases, The survival rate is improved and the taste quality of seafood (eg, texture, flavor, etc.) is improved. Example 1

[Manufacture of pellets containing dead cells of Lactobacillus plantarum L-137]

(Bacteria culture)

1% glucose, 1% yeast extract, 0.5% polypeptone, 0.2% meat extract, 0.2% sodium acetate, manganese sulfate tetrahydrate 0.001%, iron sulfate heptahydrate 0.001%, salt Lactobacillus plantarum L-137 (Accession No. FERM BP-08607) strain was inoculated into 6 L of a medium containing 0.001% and 0.05% sucrose fatty acid ester, and cultured at 32 for 24 hours. After culturing, the culture was centrifuged at 5000 rpm for 35 minutes to collect the cells. The obtained bacterial cells were well dispersed in physiological saline and centrifuged at 5000 rpm for 35 minutes, and then the supernatant was removed to collect the bacterial cells. After repeating this operation three times, the cells were dispersed in ion-exchanged water, heated at 70 ° C. for 10 minutes, and then freeze-dried to obtain about 7 g of heated dead cells. (Manufacture of pellets from dead cells)

Brown Fish Meal 65. 6 parts by weight, —Starch 3.4 parts by weight, dextrin 3.4 parts by weight, soy lecithin 4.2 parts by weight, sodium menadione hydrogen bisulfate 0.0124 parts by weight, thiamine mononitrate 0.01797 Parts by mass, riboflavin 0.05991 parts by mass, pyridoxine monohydrochloride 0.014 8 parts by mass, cyanobalamine 0.00002 parts by mass, cellulose 0.5986 9 parts by mass, piotin 0.00191 parts by mass, inositol 1. 19769 parts by mass Nicotinic acid 0.23954 parts by mass, calcium pantothenate 0.00838 6 parts by mass, folic acid 0045 parts by mass, choline chloride 2.44855 parts by mass, paraaminobenzoic acid 0.111929 parts by mass, vitamin CO. 04681 parts by mass, Mineral mix 4.3 parts by weight, inosinic acid 0.1 parts by weight, betaine 0.6 parts by weight The feed material consisting of 0.4 parts by weight, alanine and 5.0 parts by weight activated dartene was mixed well and mixed for 10 minutes at the kitchen end. To this was added vitamin-containing 鳕 liver oil (鳕 fF oil 7 parts by weight, ^ Ichirotin 0.0996 parts by weight, vitamin D ₃ 0.00301 parts by weight, α-tocopherol 0.111993 parts by weight, ultrasonic washing machine The mixture was added using Lactobacillus branulam L_137 heated dead cells are added to this to a predetermined concentration, then α-cell mouthwater is added, and the total amount is adjusted to 100 parts by mass to feed dead cells. It was. The addition concentration of the heated dead cells was set to 5 levels of 0, 0.2, 2, 20, and 200 ppm in the dead cell-containing feed, respectively.

Water was added to the dead cell-containing feed obtained above so as to be 40% by mass with respect to the feed and mixed for 10 minutes. The obtained mixture was ground twice with a chopper to prepare a pelleted feed having a diameter of 1.2 mm. This was dried in an oven to make the water content less than 10% of the total as the test feed. The composition of the mineral mix is shown in Table 1 below.

Table 1

Red sea bream larvae (weight: 0.9 ± 0. lg) were placed in a circulating 30L circular polycarbonate tank, 15 each, and 3 tanks were set for each test feed, and the test feed was taken twice a day in the morning and evening. 5-7% of the body weight was given, the water change rate was 2.4 LZ, and the water temperature was maintained at 20. 0 ° C. After 30 days of breeding, blood was collected from the tail capillaries. After preparing serum from this blood, the amount of cortisol in the serum was measured by ELISA. The results are shown in Table 2. Table 2

As can be seen from Table 2, the Lactobacillus plantarum L-1 137 strain (Lactobacillus plantarum L-137) heat-killed cells containing 0.2 ppm or more of the feed group were fed in the test group. Cortisol in red sea bream serum was significantly lower than in the control group that received a diet that did not contain dead cells. Example 2

1. Manufacture of dead cells

In a medium containing maltose 4%, polyheptone 2%, yeast extract 2%, sodium acetate 0.5%, polyoxyethylene (20) sorbitan monooleate 0.05%, manganese sulfate 002%, Lactobacillus planon Talam L-137 (Lactobacillus plantarum L-137) was cultured at 32 ° C for 24 hours, and the culture was heat-treated at 80 for 20 minutes. Thereafter, the culture solution was washed and concentrated with a filter concentration device while adding water to obtain a heated dead cell solution. Dextrin was added to the obtained heated dead cell solution and spray-dried to obtain a treated product of dead cells containing 18% of heated dead cells.

2. Manufacture of pellets

Per 100 g, brown fish meal 65.6 g, α-starch 5. 8 g, dextrin 3.4 g, cod liver oil 5.2 g, soy lecithin 2. O g, highly unsaturated fatty acid 1.0 g, vitamin mix 3.0 g, mineral mix 3.0 g, betaine 0 5 g, alanine 0.5 g, carboxymercellulose sodium salt 5.0 g, treated with dead cells produced in 1 above, 0.Olg (bacterial cell content of about 0.0019 g) An appropriate amount of tap water was added to the mixture containing 4.99 g of α-cellulose and mixed well, and a feed with a diameter of 2.5 mm was prepared at Perez Yuichi. The obtained feed was dried in an oven set at 50 ° C. until the water content became 10%, and this was used as a test feed. In addition, a control feed was prepared in the same manner as the method for producing the test feed, except that the dead cell-treated product was replaced with a micellar mouth.

The composition of the vitamin mix is shown in Table 3, and the composition of the mineral mix is shown in Table 4.

Table 3

Vitamins contained per 3 g of vitamin mix (mg)

β-strength rotin 9.63 Hitano D ₃ 0.97 Menanian monosulfite aqueous solution Narium 4.58 Ichino Noel Les 38.5 Chino Nitrate 5.78 Li ^! ヽ Nofuno 19.24 Shihi ⁰ ϊri 1 Thousand ^-:, ,, Έϋ τΈϋ ^ ΑΑ

Nnomonohydrochloric acid 4.58 N

1 Relose 192.45 Hinano 0.58 N / A 384.92 ί Nano acid 76.97 Calcium pantothenate 26.95 Folic acid 1.44 Choline chloride 786.93 Paraaminobenzoic acid 38.33 Ascorbic acid mono 2-phosphate sodium calcium 28.57

Table 4

Mineral mix per 3 g mineral (mg)

Five flounder larvae (weight: 23.5 g) were housed in 45L aquariums and bred for 1 week. At the time of breeding, the control group (number of tanks = 2) was given a control feed, and the test group (number of tanks = 2) was converted to the amount of Lactobacillus prunum lamb L-1 137 heated dead cells. The test diet was given at 0.36 mg / kg day. After breeding, the flounder larvae were exposed to the air for 20 minutes in the air at room temperature 20 ° C and humidity 70%. After being stressed, they were transferred to the recovery tank (1L) and stabilized (the fry swims normally). Time until recovery to the state) was measured.

As a result, the recovery time in the control group averaged 26.2 minutes, while the recovery time in the test group (Lactobacillus pranyurum L-1 137 strain heated dead cell intake group) The mean was 18.9 minutes. From this, Lactobacillus plantarum

It can be seen that feeding the L-137 strain heat-killed cells significantly shortened the recovery time and increased the resistance of fry to stress.

Example 3

Per 100 g, brown fish meal 70 g, hii starch 3.8 g, dextrin 3.5 g, cod liver oil 5.7 g, vitamin mix 3.0 g, mineral mix 3.0 g, bayuin 0.5 g, alanine 0.5 g, carboxymethylcellulose sodium salt 5.0 g, a mixture containing 0.01 g of the dead cell processed product prepared in Example 2 (0.0019 g as the cell content) and 4.99 g of cellulose Appropriate amount of tap water was added to and mixed well, and a feed with a diameter of 3.5 mm was prepared at Perez Yuichi. The obtained feed was dried in an oven set at 50 ° C. until the water content became 10%, and this was used as a test feed. In addition, a control feed was prepared in the same manner as the method for producing the test feed except that the dead cell-treated product was replaced with cellulose.

The mineral mix and vitamin mix were the same as in Example 2.

Seven vigorous puffy fry (weight: 300-600g) were housed in a 1-ton aquarium and bred for one week. At the time of breeding, the control group (number of tanks = 2) was converted to the control feed, and the test group (number of tanks = 2) was converted to the Lactobacillus planum lamb L-137 heat dead cell mass. The test feed was given on 0.03 emgZkgZ days. After breeding, three animals were randomly taken from each tank and made into sashimi. The taste (texture, appearance and flavor) of this sashimi was evaluated by 15 panelists. The results are shown in Table 5. Table 5

* No one respondent

Regarding the appearance, there is no difference between the two groups, but the test group is overwhelmingly preferred for the overall evaluation of the texture, flavor, and texture, appearance, and flavor. It has been shown. In particular, regarding the texture, the test group was more elastic and delicious, and the test group had a sense of crispiness and was often rated delicious. Regarding the flavor, the test group was more evaluated as having no odor.

If migratory fish such as force napachi are bred in close proximity in the aquarium, the stress load increases and the taste quality is expected to decline. However, when Lactobacillus branrum L_l 3 7 heat-killed cells were ingested, it was shown that the taste quality can be greatly improved even if the intake time is one week. From this, it can be seen that the quality of seafood can be improved in short-term seafood breeding (for example, breeding in a live fish restaurant or before shipping farmed seafood, etc.). Industrial applicability

The anti-stress agent of the present invention can effectively prevent or treat seafood stress.

Claims

Scope of request 1. Anti-stress agent for seafood characterized by containing dead lactic acid bacteria or processed product thereof.

2. Lactic acid bacteria include Lactobacillus plantarum, Lactobacillus acidophilus, Lactobacillus brevis, Lactobacillus brevis, Lactobacillus casei

(Lactobacillus c sein, Lactobacillus fermentum), Lactobacillus paracasei, Lactobacillus buchneri, Lactobacillus del brutsuki, Leckobacillus del

(Lactobacillus rhamnosus), Streptococcus thermophilus, Enterococcus Hue

(Enterococcus faecal is), Enterococcus faecium, Lactococcus lactis, Lactococcus pi ant arum, Bifidobacterium thermophi Bifidobacterium longum or Bifidobacterium longum

2. The antistress agent for fish and shellfish according to claim 1, which is (Bifidobacterium breve).

3. The antistress agent for fish and shellfish according to claim 1 or 2, wherein the dead cells of lactic acid bacteria are dead cells obtained by heat treatment of lactic acid bacteria.

4. Lactic acid bacteria killed by Lactobacillus plantarum L— 1 3 7 4. The anti-seafood product according to any one of claims 1 to 3, which is a heat-treated dead cell of (Lactobacillus plantarum L- 1 37, accession number FERM BP-08607). Stress agent.

5. The antistress agent for seafood according to any one of claims 1 to 4, wherein the seafood is a cultured fish or an ornamental fish.

6. The antistress agent for seafood according to any one of claims 1 to 5, wherein the seafood is saltwater fish or freshwater fish.

7. The antistress agent for seafood according to any one of claims 1 to 6, wherein the seafood is a larvae and juvenile fish.

8. The proportion of dead lactic acid bacteria or treated product thereof is 0.01 to 95% by mass relative to the total amount, according to any one of claims 1 to 7 The antistress agent for seafood as described.

9. A fish and seafood feed comprising the fish and shellfish antistress agent according to any one of claims 1 to 8.

10. The feed for fish and shellfish according to claim 9, wherein the concentration of dead lactic acid bacteria or a processed product thereof in the feed is more than 0 and 2000 ppm or less.

1 1. A method for preventing or treating seafood stress, comprising feeding the seafood with the antistress agent for seafood according to any one of claims 1 to 8,

1 2. Prevention of stress in fish and shellfish characterized by feeding 0.05 to 7 to 5 mg of lactic acid bacteria dead or processed product to fish and fish per day per kg of fish or seafood Method of treatment.

1 3. A method for inhibiting the elevation of serum cortisol in fish and shellfish, comprising using the antistress agent for fish and shellfish according to any one of claims 1 to 8.

1 4. A method for cultivating seafood, characterized by using the antistress agent for seafood described in claim 1.

1 5. The culture method according to claim 14, wherein the taste quality of seafood is improved.

1 6. The culture method according to claim 14 or claim 15, wherein the survival rate of the cultured seafood is improved.