CN100353979C - Chinese herbal medicinal immune intensifier mode from left-eyed flounder and right-eyed flounder - Google Patents
Chinese herbal medicinal immune intensifier mode from left-eyed flounder and right-eyed flounder Download PDFInfo
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
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- Y02A40/00—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
- Y02A40/80—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in fisheries management
- Y02A40/81—Aquaculture, e.g. of fish
- Y02A40/818—Alternative feeds for fish, e.g. in aquacultures
Abstract
The present invention discloses a Chinese herbal medicinal immune intensifier prepared from left-eyed flounders and right-eyed flounders. The present invention is characterized in that the present invention is prepared from constituents of the following share by weight: 90 to 110 of ginseng, 115 to 135 of honeysuckle flower, 115 to 135 of spine date seed, 115 to 135 of garlic, 115 to 135 of haw and 90 to 110 of schisandra. The constituents interact, the present invention has nutrition property as well as medicine property, namely that the present invention not only contains nutritive substances, but also has antibacterial activity and ingredients with other biological activity. The present invention can improve the basic metabolism of fish, promote growth and development, enhance the immunological function, prevent diseases, etc. The present invention not only overcomes harm caused by chemical disinfectant and bacteriophage of the prior art to a human body, but also solves the problems of the existing marine product vaccine, such as strong specificity and high cost. The present invention has the advantages of wide source of raw materials, low price and convenient use, and the present invention has higher social benefits and economic benefits.
Description
Technical field:
The present invention relates to a kind of Fish and use immune formulation, especially a kind of left-eyed flounder Chinese herbal medicine immunostimulant.
Background technology:
Flounder, sole class are grown fast, and meat is good, and the economic worth height is one of main fish of northern China industrialized culture, and annual production is about 8000～10000 tons.In the past, the food of Flounder, sole class was natural small fish basically, exist waste natural resources, energy utilizes shortcomings such as unreasonable.People adopt the mode of self-control forage feed for this reason, promptly be mixed and made into feedstuff with fish flour, dried small shrimps, bean cake, wheat bran, flour, yeast powder, green dish, phospholipid, premix material etc., can promptly save natural resources and economical rationality by allotment starch, bean cake, fish flour with the albumen gradient in the control feedstuff.Yet existing self-control feedstuff is the same with natural small fish, all exists the pollution breeding water body, grows the problem of cause of disease, thereby cause fish diseases popular, and due to illness do harm to and lose nearly hundred million yuan every year.
The loss that brings in order to prevent and treat disease to give birth to frequently, people can only be extensive use of various sterilizations and germ killing drugs and Aquatic product vaccine, but but exist following shortcoming: along with people are more and more higher to the safety requirements of aquatic products, many to be used for the disinfectant chemical agent disabled; Antibiotic sterilization not only can make Resistant strain increase, and drug effect reduces, but also can enrichment in the fish body, and the health of human body is produced serious harm; The Aquatic product vaccine specific is strong, can only have protection to render a service to a certain germ disease, also the kind of existing vaccine very less, complicated operation, cost an arm and a leg and can't satisfy the actual demand of aquaculture.
Summary of the invention:
The present invention's technical problems such as existing in prior technology fish immunity power is low in order to solve, the disease loss is serious provide a kind of fish immunity power that strengthens, and prevent the left-eyed flounder Chinese herbal medicine immunostimulant of disease loss.
Technical solution of the present invention is: a kind of left-eyed flounder Chinese herbal medicine immunostimulant is characterized in that the raw material weight proportioning is as follows:
Radix Ginseng 90～110
Flos Lonicerae 115～135
Semen Ziziphi Spinosae 115～135
Bulbus Allii 115～135
Fructus Crataegi 115～135
Fructus Schisandrae Chinensis 90～110.
The weight proportion of raw material is preferably:
Radix Ginseng 100
Flos Lonicerae 125
Semen Ziziphi Spinosae 125
Bulbus Allii 125
Fructus Crataegi 125
Fructus Schisandrae Chinensis 100.
Radix Ginseng is a tonification class medicine, and main component is ginsenoside Rb1, Rb2 and Rg etc., is the basis of Radix Ginseng active substance, and Radix Ginseng has human body immunity improving power, strengthens the phagocytic function of reticuloendothelial system.
Contain rich nutrient contents and pharmacological component in the Bulbus Allii, pharmacology and clinical confirmation have effects such as remarkable antibiotic, antiviral, blood fat reducing, antitumor, human body immunity improving function, blood sugar lowering, detoxifcation, free radical resisting.
The chemical constituent of Flos Lonicerae mainly contains volatile oil, flavonoid, organic acid, triterpenes, inorganic elements etc.; Pharmacological research show Flos Lonicerae have resisting pathogenic microbes, antiinflammatory, analgesic, protect the liver, effect such as antifertility, hemostasis, immunomodulating, blood fat reducing, central excitation; Be used for multiple infection, various inflammation, hyperpyrexia, hyperlipemia, tumor radiotherapy, chemotherapy xerostomia etc. clinically.
Isolated lignanoid in the Schisandraceae plant, having the active great majority of the liver protecting and ALT lowering is Dibenzocyclooctene lignan.This type of material protects the liver, induces drug metabolizing enzyme to increase function of detoxification, and antioxidation promotes protein biosynthesis and effect such as glycogenesis.Fructus Schisandrae Chinensis can make the excited and process of inhibition reinforcement of nervous system as tonic, promotes the two balance, helps neurasthenic recovery.Fructus Schisandrae Chinensis has " adaptogen " effect similar to Radix Ginseng, strengthens human body to the defence capability that non-characteristic stimulates, and the effect of anti-mattress antiinflammatory is arranged.
Semen Ziziphi Spinosae has the effect of tranquilizing soporific, and Semen Ziziphi Spinosae saponin and flavone are effective ingredient wherein, and wherein { jujubosid} has comprised A, B, B1 and Saponin etc. to Semen Ziziphi Spinosae saponin.
Fructus Crataegi is a rosaceous plant, and Chinese medicine thinks that Fructus Crataegi has removing food stagnancyization to stagnate, spleen reinforcing is good for the stomach, the function of activating blood circulation to dissipate blood stasis.Modern studying data at home and abroad proves that organic acid that Fructus Crataegi contains and flavone compound have more many-sided effect, comprises the treatment arrhythmia, blood fat reducing, and blood pressure lowering, anti-platelet aggregations etc. are to the effect of painstaking effort system; Aid digestion in addition, diuresis, preventing phlegm from forming and stopping coughing and effect such as anticancer.
Each component interaction of the present invention has two kinds of attributes of nutrition and medicine concurrently, promptly contains nutrient substance, has the composition of antibacterial activity and other biologic activity again, can improve the metabolism of Fish matrix, promotes growth promoter, improve its immunologic function, disease preventing and treating etc.The present invention has promptly overcome the chemosterilant of prior art and the harm that antibiotics brings for people's human body, the existing high specificity of existing Aquatic product vaccine, problem that cost is high have been solved again, have raw material sources advantage extensive, cheap, easy to use, have high social and economic benefit.
The specific embodiment:
Embodiment 1:
Get Radix Ginseng 90～110 grams, Flos Lonicerae 115～135 grams, Semen Ziziphi Spinosae 115～135 grams, Bulbus Allii 115～135 grams, Fructus Crataegi 115～135 grams, Fructus Schisandrae Chinensis 90～110 grams, can fry in shallow oil into medicinal liquid according to a conventional method.Promptly soaked 1 hour, add 1 liter in water by per 1 00 gram raw materials after, big fire is boiled 30 minutes, littler fire and was boiled 30 minutes, pours out medicinal liquid; Adding water in medicine, big fire is boiled 30 minutes, littler fire and was boiled 30 minutes, pours out medicinal liquid; After twice medicinal liquid mixing and being concentrated into 1.0～1.5 liters, sneak in 220～270 kilograms of feedstuffs, add water at last and become pasty state, be extruded into strip with extruder, behind the natural air drying, make the granule of corresponding size by fish length, the pack stored under refrigeration is promptly made the feedstuff that is mixed with Chinese herbal medicine.Its feeding method is with existing forage feed method, and nutrition such as protein, lipid and saccharide are essential sufficient.
Embodiment 2:
Get Radix Ginseng 100 grams, Flos Lonicerae 125 grams, Semen Ziziphi Spinosae 125 grams, Bulbus Allii 1 25 grams, Fructus Crataegi 125 grams, Fructus Schisandrae Chinensis 100 grams, can fry in shallow oil into medicinal liquid according to a conventional method.Promptly soaked 1 hour, add 1 liter in water by per 100 gram raw materials after, big fire is boiled 30 minutes, littler fire and was boiled 30 minutes, pours out medicinal liquid; Adding water in medicine, big fire is boiled 30 minutes, littler fire and was boiled 30 minutes, pours out medicinal liquid; After twice medicinal liquid mixed and being concentrated into 1.25 liters, sneak in 250 kilograms of feedstuffs, add water at last and become pasty state, be extruded into strip with extruder, behind the natural air drying, make the granule of corresponding size by fish length, the pack stored under refrigeration is promptly made the feedstuff that is mixed with Chinese herbal medicine.Its feeding method is with existing forage feed method, and nutrition such as protein, lipid and saccharide are essential sufficient.
Experimental result:
Experiment is carried out in (100 square metres of every open area) in 8 mouthfuls of water mud sumps.4 mouthfuls of ponds embodiment 1,2 (feedstuff that contains Chinese herbal medicine) that throws something and feeds wherein, throw something and feed and do not add the feedstuff of Chinese herbal medicine in all the other 4 mouthfuls of ponds, in contrast; Put big specification fish (390～400g/ tail) in 2 mouthfuls of ponds in a suitable place to breed; Specification fish (280～320 g/ tail) in putting in a suitable place to breed in 2 mouthfuls of ponds; Small dimension fish (95～110g/ tail) (table 1) is put in all the other 4 mouthfuls of ponds in a suitable place to breed.Adopt the batch production daily management, every day, bait throwing in twice, adopts nature seawater flowing water to culture.Experiment periods by August 2 to October 24, last 84 days.
Adopt Paralichthys olivaceus 4～6 tails of different size in every month, measure protease, lipase and diastatic activity in stomach, the intestinal regulating liver-QI pancreas; The vigor of acid phosphatase, alkali phosphatase, lysozyme and SOD in serum is measured in the docking blood sampling.
The growth of Paralichthys olivaceus, survive and feed efficiency
Table 1 Chinese herbal feed is raised the situation of putting in a suitable place to breed of Paralichthys olivaceus experiment
| Pond number | Matched group | Experimental group | ||||
| Mantissa | Total length (cm) | Body weight g | Mantissa | Total length (cm) | Body weight g | |
| I-7 I-8 (grass) | 1089 | 33 | 400 | 1026 | 33.1 | 390 |
| II-9 II-11 (grass) | 1212 | 29.8 | 280 | 1280 | 31.4 | 320 |
| II-15-1 * II-15-2 II-15-3 *(grass) II-15-4 (grass) | 350 350 | 20.2 21.6 | 95 110 | 350 350 | 20.6 20.8 | 108 102 |
* for suffering from the sick fish of lymphocyst
The big specification Paralichthys olivaceus average daily gain rate (SGR) of adding Chinese herbal feed of ingesting is 1.28%, and the big specification Paralichthys olivaceus that does not add Chinese herbal feed of ingesting is 1.03%, and the former increases by 24.27% than the latter; The middle specification Paralichthys olivaceus rate of body weight gain that adds Chinese herbal feed of ingesting is 0.81%, and the matched group Paralichthys olivaceus is 0.79%, and the former increases by 10.5% than the latter; The rate of body weight gain of healthy small dimension Paralichthys olivaceus feeding experiment feedstuff is 1.13%, and matched group is 1.25%, and the former increases weight not as good as the latter.The above results shows that Chinese herbal medicine strengthens along with the increase of fish specification the growth promoting function of Paralichthys olivaceus.
The average survival of the Paralichthys olivaceus that contains Chinese herbal medicine of ingesting is 98.03%, and the survival rate of the control feed Paralichthys olivaceus of ingesting is 97.00%, and the former survival rate is higher than the latter.
The feed coefficient of Paralichthys olivaceus descends with the increase of fish specification, raises with the decline of water temperature.The Paralichthys olivaceus feed coefficient that contains Chinese herbal feed of ingesting is 3.20, and the feed coefficient of experimental group has reduced by 8.75% (table 2) than matched group.
Table 2 with Chinese herbal feed feed Paralichthys olivaceus growth, survive and feed coefficient
| Pond number | On August 30th, 2003 | ||||||
| Total length (cm) | Increase (cm) | Body weight (g) | Weightening finish (g) | Day instantaneous rate of body weight gain SGR (%) | Survival rate (%) | Feed coefficient | |
| I-7 I-8 (grass) II-9 II-11 (grass) II-15-1 * II-15-2 II-15-3 *(grass) II-15-4 (grass) | 36.2 37.3 32.2 33.7 22.8 23.5 21.8 24.01 | 3.2 4.2 2.4 2.3 2.7 1.9 1.2 3.2 | 540 570 380 420 130 150 120 150 | 141 180 100 100 39 41 13 48 | 1.03 1.28 1.03 0.93 1.21 1.07 0.38 1.31 | 98.62 99.02 99.17 99.37 90.2 99.42 90.2 99.14 | 2.07 1.64 2.58 2.47 3.58 3.13 2.44 2.75 |
| On JIUYUE 27th, 2003 | |||||||
| II-9 II-11 (grass) II-15-1 * II-15-2 II-15-3 *(grass) II-15-4 (grass) | 33.9 35.2 26 26.7 25.3 26.4 | 1.7 1.57 3.18 3.2 3.5 2.35 | 440 510 200 220 180 210 | 59 93 69 64 57 57 | 0.54 0.68 1.46 1.25 1.36 1.14 | 98.66 98.82 90 96.78 92.9 99.12 | 4.11 2.49 2.7 2.46 3.27 2.79 |
| On October 28th, 2003 | |||||||
| II-15-1 * II-15-2 II-15-3 *(grass) II-15-4 (grass) | 28.5 28.2 27.1 28.3 | 2.53 1.49 1.79 1.94 | 270 270 230 260 | 68 49 56 58 | 1.07 0.74 1.04 0.93 | 98.9 99.09 99.67 99.41 | 3.37 3.95 3.96 3.23 |
Digestive enzyme in Paralichthys olivaceus stomach, the intestinal
Compare with matched group, after Paralichthys olivaceus was ingested and added the feedstuff of Chinese herbal medicine, stomach, erepsin activity had improved 4.14～23.76% (tables 3) and 4.89～29.53% (tables 4) respectively; Stomach, entero-amylase activity have improved 1.68～5.39% (tables 5) and 3.95～16.85% (tables 6) respectively.
Table 3 Chinese herbal medicine is to the influence (n=6) of pepsin activity
Matched group [10
-2Unit/ (minmg)] experimental group [10
-2Unit/ (minmg)]
Big fish 20.75 ± 0.89 23.67 ± 1.13 20.99 ± 1.58 26.43 ± 1.64
*
Middle fish 17.09 ± 1.63 20.37 ± 1.92 22.83 ± 1.56 18.32 ± 1.25 21.89 ± 1.3 25.91 ± 2.07
*
Be good for little by 7.93 ± 2.12 9.89 ± 1.68 13.55 ± 2.24 22.67 ± 1.31 7.82 ± 1.92 12.24 ± 1.62
*14.21 ± 3.31
24.74±1.82
*
Sick little by 7.72 ± 1.98 9.21 ± 2.23 12.55 ± 1.35 21.44 ± 1.16 7.87 ± 0.57 8.78 ± 2.76 13.07 ± 1.43 23.73 ± 1.34
*
Annotate: " strong little " represents healthy small fish in the table, and " sick little " represents ill small fish, and " * " represents significant difference (P＜0.05), and the back together.
Table 4 Chinese herbal medicine is to the active influence of erepsin (n=6)
Matched group [10
-2Unit/ (minmg)] experimental group [10
-2Unit/ (minmg)]
Big fish 16.33 ± 2.57 20.97 ± 1.09 16.42 ± 1.98 23.34 ± 1.15
*
Middle fish 12.97 ± 1.3 15.96 ± 2.19 17.13 ± 2.1 13.3 ± 1.67 16.74 ± 2.44 19.98 ± 1.12
*
Be good for little by 5.01 ± 2.85 6.13 ± 1.49 8.57 ± 1.71 11.69 ± 1.76 5.12 ± 2.12 7.94 ± 1.32
*10.67 ± 2.61 14.69 ± 1.13
*
Sick little by 5.09 ± 2.42 6.15 ± 2.92 8.48 ± 2.48 11.25 ± 2.34 4.97 ± 2.42 5.87 ± 2.23 10.07 ± 2.34 13.39 ± 2.19
Table 5 Chinese herbal medicine is to the influence (n=6) of stomach amylase activity
| Matched group (enzyme activity/unit of activity) | Experimental group (enzyme activity/unit of activity) | |||||||
| The strong minor illness of fish is little in the big fish | 4.43±0.23 3.27±0.35 1.22±0.94 1.15±0.64 | 4.94±0.37 4.19±1.14 1.76±0.58 1.79±0.84 | 4.77±0.67 2.41±0.97 2.47±0.73 | 3.47±1.28 3.54±1.21 | 4.57±0.25 3.19±0.35 1.26±0.72 1.07±0.62 | 5.07±0.29 4.31±0.68 1.81±1.16 1.39±1.62 | 4.85±0.78 2.54±0.89 2.55±0.94 | 3.55±1.74 3.63±1.43 |
Table 6 Chinese herbal medicine is to the active influence of entero-amylase (n=6)
Matched group (enzyme activity/unit of activity) experimental group (enzyme activity/unit of activity)
Big fish 15.75 ± 2.65 17.95 ± 2.43 15.97 ± 2.87 19.12 ± 2.98
Middle fish 10.75 ± 2.13 12.79 ± 2.68 14.17 ± 2.31 9.97 ± 1.86 13.03 ± 2.05 16.22 ± 2.16
Be good for little by 3.67 ± 1.51 4.45 ± 1.61 7.85 ± 1.94 9.56 ± 1.62 3.49 ± 1.87 5.20 ± 1.94 8.37 ± 2.17
10.09±3.13
Sick little by 3.79 ± 1.66 4.33 ± 1.72 7.47 ± 1.96 9.63 ± 2.16 3.68 ± 1.24 4.29 ± 1.86 8.54 ± 2.06
10.01±2.54
The Paralichthys olivaceus non-specific immunity
Paralichthys olivaceus is ingested, and antalzyme activity has improved 12.42～17.56% (tables 7) behind the feedstuff that adds Chinese herbal medicine; SOD in serum risen 4.64～24.71%, 10.01% (table 8); The Total albumen content has improved 7.07～11.54% (tables 9);
Table 7 Chinese herbal medicine is to the active influence of serum lysozyme (n=4)
| Matched group | Experimental group | |||||
| The strong minor illness of fish is little in the big fish | 0.467±0.13 0.461±0.20 0.421±0.24 0.437±0.21 | 0.451±0.23 0.429±0.18 0.353±0.13 0.393±0.17 | 0.364±0.23 0.280±0.11 0.272±0.12 | 0.472±0.11 0.459±0.12 0.483±0.27 0.440±0.14 | 0.507±0.19 0.495±0.28 0.415±0.21 0.409±0.17 | 0.427±0.19 0.325±0.15 0.328±0.14 |
Annotate: " strong little " represents healthy small fish in the table, and " sick little " represents ill small fish, and the back together.
Table 8 Chinese herbal medicine is to the influence (n=4) of activity of SOD in serum
| Matched group | Experimental group | |||||
| The strong minor illness of fish is little in the big fish | 143.95±15.24 69.66±8.97 24.69±6.04 28.12±7.86 | 163.58±18.95 118.85±10.36 47.22±9.37 53.06±11.26 | 122.98±13.21 58.44±8.19 59.64±13.98 | 140.73±17.64 67.87±16.23 27.5±7.47 25.87±9.24 | 171.17±19.82 123.69±19.98 58.98±12.38 46.07±12.43 | 134.16±18.65 64.29±12.35 63.60±15.9 |
Table 9 Chinese herbal medicine is to the active influence of total serum protein (n=4)
| Matched group (mg/ml) | Experimental group (mg/ml) | |||||
| The strong minor illness of fish is little in the big fish | 17.35±2.6 15.77±1.9 12.91±1.2 13.19±1.5 | 19.93±3.6 16.87±2.3 13.74±1.5 14.08±2.4 | 17.16±3.1 14.06±2.8 14.91±2.1 | 17.08±2.8 15.88±2.3 14.04±1.6 12.89±2.5 | 21.34±3.4 17.76±2.8 14.93±2.1 14.99±1.9 | 19.14±3.4 15.16±2.6 15.83±2.7 |
Claims (2)
1. left-eyed flounder Chinese herbal medicine immunostimulant is characterized in that raw material and weight proportion are as follows:
Radix Ginseng 90～110
Flos Lonicerae 115～135
Semen Ziziphi Spinosae 115～135
Bulbus Allii 115～135
Fructus Crataegi 115～135
Fructus Schisandrae Chinensis 90～110.
2. left-eyed flounder Chinese herbal medicine immunostimulant according to claim 1 is characterized in that the weight proportion of raw material is as follows:
Radix Ginseng 100
Flos Lonicerae 125
Semen Ziziphi Spinosae 125
Bulbus Allii 125
Fructus Crataegi 125
Fructus Schisandrae Chinensis 100.
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| CN101480432B (en) * | 2008-11-19 | 2011-06-08 | 广东海洋大学 | Compound Chinese herbal medicine immunopotentiator for Rachycentron canadum |
| CN101658252B (en) * | 2009-09-17 | 2013-04-17 | 大连水产学院 | Food organism additive for cultivating stichopus japonicus |
| CN101912062B (en) * | 2010-07-26 | 2012-12-05 | 大连海宝渔业有限公司 | Feed for promoting maturation of parent sea cucumber of stichopus japonicus |
| CN102524596B (en) * | 2012-02-15 | 2013-05-29 | 南京农业大学 | Immune-enhanced feed for Megalobrama amblycephala and production method for immune-enhanced feed |
| CN102715365B (en) * | 2012-05-21 | 2013-12-25 | 高雷 | Fish feed additive and preparation method |
| CN105594992A (en) * | 2014-11-25 | 2016-05-25 | 广西大学 | Health care type feed additive for fishes and shrimps with anti-microbial and disease-preventing effects |
| CN105029105A (en) * | 2015-08-28 | 2015-11-11 | 青岛海之源智能技术有限公司 | Low-cost and easy-to-eat mullet compound feed and manufacturing method thereof |
| CN107647087A (en) * | 2017-11-21 | 2018-02-02 | 五河县金塘水产养殖有限公司 | A kind of bait for strengthening carp kidney cell resistance against diseases |
| CN115334898A (en) * | 2020-05-21 | 2022-11-11 | 汉坰妊 | Environment-friendly growth auxiliary and preparation method thereof |
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| 天然中草药药理及其在水产业的应用 谢仲权.北京水产，第3期 1998 * |
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| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant | ||
| C56 | Change in the name or address of the patentee |
Owner name: DALIAN HAIBAO FISHERY COMPANY LTD. Free format text: FORMER NAME: DALIAN TAIPINGYANG AQUATIC PRODUCTS CO., LTD. |
|
| CP01 | Change in the name or title of a patent holder |
Address after: 116045 Bai Lanzi, Tieshan Town, Lushunkou District, Liaoning, Dalian Patentee after: Dalian Haibao Fishery Company Ltd. Address before: 116045 Bai Lanzi, Tieshan Town, Lushunkou District, Liaoning, Dalian Patentee before: Dalian Pacific Seafood Co., Ltd. |
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| C17 | Cessation of patent right | ||
| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20071212 Termination date: 20120929 |